A device for enhancing the aroma and quality of tobacco by strengthening cellulose degradation and its usage method

Abstract

This application relates to the field of tobacco flavoring technology, providing a tobacco flavoring and quality improvement device and its method of use for enhancing cellulose degradation. The device includes: a housing, electrodes, a power supply system, a water replenishment and humidification system, and a heat preservation system. The housing is equipped with several degradation reaction zones. Tobacco and microbial agents are mixed and added to the degradation reaction zones. Each degradation reaction zone is equipped with an electrode, which is electrically connected to the power supply system. The water replenishment and humidification system sprays water into each degradation reaction zone, and the heat preservation system heats and maintains the temperature of each degradation reaction zone. The electrodes divide the housing into multiple appropriately sized degradation reaction zones. An external low-voltage electric field enhances the microbial degradation activity, breaking down the energy barrier for microbial degradation of cellulose and improving the degradation of hemicellulose, which is difficult to bioutilize. Simultaneously, it solves the problem of excessively high external power supply voltage due to the large volume of the degradation reaction zones, ensuring the feasibility and economy of the process.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of tobacco flavoring, and more particularly to a device for enhancing cellulose degradation of tobacco flavoring and a method thereof. BACKGROUND

[0002] A new electronic cigarette requires its main component to be able to decompose and release smoke at 250°C. However, the macromolecular substances such as cellulose and hemicellulose in tobacco have complex structures, high stability, and a weight loss temperature much higher than 250°C. Therefore, it is urgent to explore a degradation technology suitable for cellulose and hemicellulose in tobacco, so that they can be degraded into small molecular substances that are easy to thermally decompose. Modern biotechnology is a technology that uses functional microorganisms and special biological enzymes to provide a certain function. It has the advantages of mild operating conditions, low energy consumption, and no pollution, and is considered to be a macromolecular organic matter degradation method with broad development prospects. However, cellulose has a complex structure and is difficult to be rapidly degraded by biology, so it is necessary to couple biology with other physical means to improve the degradation efficiency of cellulose.

[0003] Electric field enhanced biodegradation technology is a technology that can efficiently enhance the metabolic activity of microorganisms and improve the catalytic activity of biological enzymes. It has the advantages of strong operability, high efficiency, no additional substances introduced, and no toxic by-products generated. Electric field coupled biotechnology has been widely used in the degradation of macromolecular organic matter in water, soil and other environments, and good results have been achieved. However, there is no report on the use of electric field coupled microbial technology to degrade cellulose in tobacco to achieve tobacco flavoring and quality improvement. SUMMARY

[0004] In view of the above shortcomings of the prior art, the purpose of the embodiments of the present application is to provide a device for enhancing cellulose degradation of tobacco flavoring and quality improvement. It combines microbial degradation technology with low-current electric field enhancement technology, uses electrodes to divide the box into several degradation reaction zones of appropriate size, enhances the microbial degradation activity by applying a low-voltage electric field, breaks the energy barrier of microbial degradation of cellulose, and improves the degradation of cellulose and hemicellulose that is difficult to be biologically utilized. At the same time, it solves the problem of excessive voltage of the external power supply caused by the large pool capacity of the degradation reaction zone, ensuring the feasibility and economy of the process.

[0005] To achieve the above purpose, the technical scheme adopted by the present application is to provide a device for enhancing cellulose degradation of tobacco flavoring and quality improvement, comprising: a box, electrodes, a power supply system, a water supplementing and moisturizing system, and a heat preservation system. The box is provided with a plurality of degradation reaction zones, the tobacco to be treated and microbial inoculum are mixed and put into the degradation reaction zones, each degradation reaction zone is provided with electrodes, the electrodes are electrically connected with the power supply system, the water supplementing and moisturizing system sprays water into each degradation reaction zone, and the heat preservation system heats and preserves each degradation reaction zone.

[0006] In one embodiment, a plurality of symmetric clamping grooves are arranged in the box, and the electrodes are sheet electrodes, and one sheet electrode is inserted into a symmetric set of clamping grooves.

[0007] In one embodiment, the electrodes are graphite electrodes, platinum electrodes, titanium electrodes, copper electrodes or stainless steel electrodes.

[0008] In one embodiment, the cathode and the anode of the electrodes are arranged at a distance of 1-20 cm.

[0009] In one embodiment, the electric field strength provided by the power supply system is 0.5-5 V / cm.

[0010] In one embodiment, the microbial agent is a cellulose and hemicellulose degradation bacteria mixed preparation, and at least one of Bacillus subtilis, Cellulomonas, Cellvibrio, Cytophaga, Clostridium, Bacteroides succinogenes and Cellvibrio fibrosolysing butyric acid bacteria.

[0011] In one embodiment, the addition amount of the microbial agent is 5%-30%, the humidity of the degradation reaction zone is 60%-70%, the temperature of the degradation reaction zone is 20-40℃, and the reaction time of the degradation reaction zone is 4-48 h.

[0012] In one embodiment, the water supply and moisturizing system comprises a water tank, a water pump, a control valve, a metering pump, a water distribution pipe and a humidity sensor, the water distribution pipe connects the water tank, the water pump, the control valve and the metering pump, a water distribution plate is arranged on the side wall of the box, a plurality of interconnected water inlet holes and water outlet holes are formed in the water distribution plate, and the water distribution pipe is connected with the water inlet holes.

[0013] In one embodiment, the heat preservation system comprises an electric heater and a temperature sensor.

[0014] Another object of the present application is to provide a use method of the tobacco flavoring and quality improving device for strengthening cellulose degradation, based on the above-mentioned tobacco flavoring and quality improving device for strengthening cellulose degradation, the use method comprising the following steps:

[0015] S1. According to the amount of tobacco to be treated, a proper amount of electrodes are inserted into the box, and the cathode and the anode of the electrodes are alternately placed to divide the box into a plurality of degradation reaction zones, and the cathode and the anode are connected to the power supply;

[0016] S2. A plurality of portions of tobacco materials with a certain mass and a proper amount of microbial agents are weighed, the microbial agent is 5%-20% of the mass of the tobacco, and the tobacco materials and the microbial agents are stirred and mixed uniformly;

[0017] S3, several parts of the mixture in step S2 are added to each of the degradation reaction zones, and the mixture fills each of the degradation reaction zones, and the top cover of the box is closed;

[0018] S4, the water supply and moisturizing system is started, the control valve and the humidity sensor are started, and the humidity in each degradation reaction zone is adjusted to 60%-70%;

[0019] S5, while the water supply and moisturizing system is started, the electric heater and the temperature sensor of the heat preservation system are started, the temperature in each degradation reaction zone is adjusted, and is stabilized at 37℃;

[0020] S6, after the humidity and temperature reach the predetermined conditions, the power switch is turned on, and the external electric field provided by the power supply is adjusted to a predetermined strength;

[0021] S7, according to the required reaction time, after the reaction is completed, the power supply is turned off, the box is opened, and the degraded tobacco mixture is taken out and washed with clean water.

[0022] The tobacco flavoring and quality improving device for strengthening cellulose degradation and the use method thereof provided by the application have the beneficial effects that:

[0023] 1. The microbial degradation technology is combined with the low-current electric field strengthening technology, the microbial degradation activity is strengthened by an external low-voltage electric field, the energy barrier of the microbial degradation of tobacco cellulose is broken, the tolerance of the microorganism to the harmful components of tobacco is enhanced, the degradation of the cellulose and hemicellulose which is difficult to be biologically utilized is improved, and the tobacco smoke release temperature is effectively reduced;

[0024] 2. The box is divided into multiple degradation reaction zones of appropriate size by electrodes, the problem of excessive external power supply voltage caused by the large pool capacity of the degradation reaction zone is solved, and the feasibility and economy of the process are ensured.

[0025] 3. The microbial degradation technology is combined with the low-current electric field strengthening technology, no chemical reagent needs to be introduced, the pollution to the tobacco is effectively prevented, the cleaning process of the degraded tobacco is greatly simplified, and the safety and efficiency of the tobacco quality improving process are ensured. BRIEF DESCRIPTION OF DRAWINGS

[0026] In order to more clearly illustrate the technical solutions in the embodiments of the application, the drawings needed to be used in the embodiments or the prior art description will be briefly introduced. Obviously, the drawings in the following description are only some embodiments of the application, and other drawings can be obtained by those skilled in the art without creative labor.

[0027] Figure 1A simplified structure diagram of a tobacco flavoring and quality improving device for strengthening cellulose degradation provided by an embodiment of the present application is shown in the figure.

[0028] Figure 2 A simplified structure diagram of a box in a tobacco flavoring and quality improving device for strengthening cellulose degradation provided by an embodiment of the present application is shown in the figure.

[0029] Figure 3 A structure diagram of a water distribution plate in a tobacco flavoring and quality improving device for strengthening cellulose degradation provided by an embodiment of the present application is shown in the figure.

[0030] Figure 4 A diagram of the degradation rate of tobacco cellulose by microorganisms under the condition of no external electric field and different electric field intensities in an embodiment of the present application is shown in the figure.

[0031] Figure 5 A diagram of the influence of tobacco cellulose degradation rate in a biological two-pair electric field intensified microbial degradation process in an embodiment of the present application is shown in the figure.

[0032] Figure 6 A diagram of the degradation efficiency of cellulose under the condition of a single electric field in an embodiment of the present application is shown in the figure.

[0033] Figure 7 A diagram of the influence of external electric field on the ATP metabolic activity of cellulose-degrading bacteria in an embodiment of the present application is shown in the figure.

[0034] Figure 8 A diagram of the influence of external electric field on the SOD enzyme activity of cellulose-degrading bacteria in an embodiment of the present application is shown in the figure.

[0035] Figure 9 A diagram of the influence of external electric field on the abundance of cellulose-degrading bacteria in an embodiment of the present application is shown in the figure.

[0036] In the figure, various reference signs are as follows:

[0037] 1, box; 11, clamping groove; 2, negative electrode; 3, positive electrode; 4, power supply system; 5, water replenishing and moisturizing system; 51, water distribution plate; 511, water inlet hole; 512, water outlet hole; 6, heat preservation system; 61, electric heater. DETAILED DESCRIPTION

[0038] In order to make the technical problems, technical solutions and beneficial effects of the present application clearer, the present application will be further described in detail below in combination with the figures and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and do not limit the present application.

[0039] It should be noted that when an element is referred to as being "fixed" or "set" on another element, it can be directly on the other element or indirectly on the other element. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or indirectly connected to the other element.

[0040] It should be understood that the terms "length", "width", "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and therefore cannot be understood as indicating or implying that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application.

[0041] In addition, the terms "first", "second" are only for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the technical features indicated. Therefore, the features defined with "first", "second" can explicitly or implicitly include one or more of the features. In the description of the present application, the meaning of "multiple" is two or more, unless otherwise specifically limited.

[0042] As Figures 1-3 As shown in the figure, a tobacco flavoring and quality improving device for strengthening cellulose degradation is provided. The device comprises a box 1, electrodes, a power supply system 4, a water supplementing and moisturizing system 5, and a heat preservation system 6. The box 1 is provided with a plurality of degradation reaction zones, and the tobacco to be treated and microbial inoculum are mixed and put into the degradation reaction zones. Each degradation reaction zone is provided with electrodes, which include cathodes 2 and anodes 3. The electrodes are electrically connected to the power supply system 4 to realize the electrification of the electrodes and the adjustment of the voltage. The power supply system 4 is a conventional power supply that can adjust the voltage. The water supplementing and moisturizing system 5 sprays water into each degradation reaction zone to control the humidity, and the heat preservation system 6 heats and preserves each degradation reaction zone to control the temperature.

[0043] In the present embodiment, a plurality of symmetrical clamping grooves 11 are provided in the box 1, and the electrodes are sheet-shaped electrodes. One sheet-shaped electrode is inserted into a symmetrical set of clamping grooves 11, which improves the installation efficiency of the electrodes.

[0044] In the present embodiment, the electrodes are graphite electrodes, platinum electrodes, titanium electrodes, copper electrodes, or stainless steel electrodes.

[0045] In the present embodiment, the cathodes 2 and the anodes 3 of the electrodes are staggered and the distance between them is 1-20 cm. Preferably, the distance between the cathodes 2 and the anodes 3 is 5-10 cm.

[0046] In the embodiment, the electric field strength provided by the power supply system 4 is 0.5-5V / cm, preferably, the electric field strength is 2V / cm-3.7V / cm.

[0047] In the embodiment, the microbial agent is a cellulose and hemicellulose degradation bacteria mixed preparation, and at least includes one of Bacillus subtilis, Cellulomonas, Cellvibrio, Cytophaga, Clostridium, Bacteroides succinogenes and Cellvibrio fibrosolysus.

[0048] In the embodiment, the addition amount of the microbial agent is 5%-30% of the mass of the tobacco, preferably 5%-20%; the humidity of the degradation reaction zone is 60%-70%; the temperature of the degradation reaction zone is 20°C-40°C, preferably 37°C; and the reaction time of the degradation reaction zone is 4h-48h, preferably 24h.

[0049] In the embodiment, the water supplementing and moisturizing system 5 includes a water tank, a water delivery pump, a control valve, a metering pump, a water distribution pipe and a humidity sensor. The water distribution pipe connects the water tank, the water delivery pump, the control valve and the metering pump. The side wall of the box body 1 is provided with a water distribution plate 51. A plurality of water inlet holes 511 and water spraying holes are formed in the water distribution plate 51. The water distribution pipe is connected with the water inlet holes 511, and water is sprayed out of the water spraying holes. The humidity sensor is used to sense the humidity of the degradation reaction zone in the box body 1. The metering pump is used to measure the water supplementing amount. The water delivery pump is a power source. The control valve is used to control the opening and closing of the water distribution pipe.

[0050] In the embodiment, the heat preservation system 6 includes electric heaters 61 and temperature sensors. The electric heaters 61 and the temperature sensors are arranged in multiple intervals. The temperature sensors are used to sense the temperature in the degradation reaction zone. The electric heaters 61 are used to heat.

[0051] In the embodiment, the bottom plate and the top cover of the box body 1 are detachable sealing structures. The purpose is to facilitate material adding and discharging. The installation mode of the bottom plate and the top cover can be a conventional mode such as plug-in or rotation. It is a conventional installation mode, and will not be described in detail here.

[0052] In the embodiment, a use method of the tobacco flavoring and quality improving device for strengthening cellulose degradation is also provided. The use method is realized based on the above-described tobacco flavoring and quality improving device for strengthening cellulose degradation. The use method includes the following steps:

[0053] S1, according to the amount of tobacco to be treated, a proper number of electrodes are inserted into the box body 1; and the cathode 2 and the anode 3 of the electrodes are alternately placed to divide the box body 1 into a plurality of degradation reaction zones. The cathode 2 and the anode 3 are connected to the power supply system 4;

[0054] S2, take several portions of a certain mass of tobacco material and an appropriate amount of microbial inoculum, the microbial inoculum is 5%-20% of the mass of tobacco, and mix them evenly;

[0055] S3, add the mixture in step S2 to each degradation reaction zone, and fill the degradation reaction zones with the mixture, close the top cover of the box 1, and make the box 1 in a sealed environment;

[0056] S4, start the water pump, control valve and humidity sensor of the water supplementing and moisturizing system 5, adjust the humidity in each degradation reaction zone to 60%-70%;

[0057] S5, start the electric heater 61 and temperature sensor of the heat preservation system 6 while starting the water supplementing and moisturizing system 5, adjust the temperature in each degradation reaction zone, and stabilize it at 37℃;

[0058] S6, after the humidity and temperature reach the predetermined conditions, turn on the switch of the power supply system 4, adjust the applied electric field provided by the power supply system 4 to a predetermined intensity, and start operation;

[0059] S7, according to the required reaction time, after the reaction is completed, turn off the power supply system 4, open the bottom plate of the box 1, take out the tobacco material mixture after reaction and degradation, and wash it with clean water for subsequent use.

[0060] Specifically, the embodiment also provides a set of tests of specific embodiment 1 and comparative examples 1-4, and the specific parameters are as follows: Specific embodiment 1:

[0062] A method for using a tobacco flavoring and quality improving device for strengthening cellulose degradation, the steps are as follows:

[0063] S1, the number of electrodes is 4, and the 4 stainless steel electrodes are inserted into the clamping groove 11 of the box 1; the four electrodes are two positive electrodes and two negative electrodes staggered, and the two side plates of the box are combined as a positive electrode and a negative electrode to form three degradation reaction zones in the box;

[0064] S2, take 100g of tobacco material and 10g of microbial inoculum, mix them evenly, and take 3 portions of the same;

[0065] S3, add the mixture in step S2 to each degradation reaction zone in step S1, and fill the degradation reaction zone with the mixture, and close the top cover;

[0066] S4, start the water pump, control valve and humidity sensor of the water supplementing and moisturizing system 5, adjust the humidity in each degradation reaction zone to 60%-70%;

[0067] S5, open the water supply and moisturizing system 5, open the electric heater 61 and temperature sensor of the heat preservation system 6, adjust the temperature in each degradation reaction zone, and stabilize at 37℃;

[0068] S6, after the humidity and temperature reach the predetermined conditions, open the switch of the power supply system 4, and adjust the applied electric field strength provided by the power supply system 4 to 2V / cm, and start running;

[0069] S7, after running for 24h, close the power supply system 4, open the bottom plate of the box 1, take out the degraded tobacco material mixture, and wash it with clean water for use.

[0070] Comparative Example 1:

[0071] A method for degrading tobacco cellulose by microorganisms, the steps are as follows:

[0072] S1, take 100g of tobacco material and 10g of microbial agent, and place them in a conical flask, and mix them evenly;

[0073] S2, spray tap water on the mixed material in the conical flask, and adjust the moisture content to 60%-70%;

[0074] S3, place the conical flask in a constant temperature incubator, and adjust the temperature to 37℃;

[0075] S4, after running for 24h, take out the degraded tobacco material mixture, and wash it with clean water for use.

[0076] Comparative Example 2:

[0077] A method for degrading tobacco cellulose by low-voltage electric field electrolysis, the steps are as follows:

[0078] S1, the number of electrodes is 4, and the 4 stainless steel electrodes are inserted into the clamping groove 11 of the box 1;

[0079] S2, take 100g of tobacco material, and take 3 identical parts;

[0080] S3, add several parts of the mixed material in step S2 to each degradation reaction zone, and fill the mixed material in each degradation reaction zone, close the top cover of the box 1, and make the box 1 in a sealed environment;

[0081] S4, open the water pump, control valve and humidity sensor of the water supply and moisturizing system 5, and adjust the humidity in each degradation reaction zone to 60%-70%;

[0082] S5, open the water supply and moisturizing system 5, open the electric heater 61 and temperature sensor of the heat preservation system 6, adjust the temperature in each degradation reaction zone, and stabilize at 37℃;

[0083] S6, after the humidity and temperature reach the predetermined conditions, open the switch of the power supply system 4, adjust the applied electric field strength provided by the power supply system 4 to 2V / cm, and start running work;

[0084] S7, after running for 24h, close the power supply system 4, open the bottom plate of the box body 1, take out the degraded tobacco material mixture, and simply wash it with clean water for use.

[0085] Comparative Example 3:

[0086] A method for low-voltage electric field assisted microbial degradation of tobacco cellulose, the steps are as follows:

[0087] S1, the number of electrodes is 4, and the 4 stainless steel electrodes are inserted into the clamping groove 11 of the box body 1;

[0088] S2, take 100g of tobacco material and 10g of microbial agent, mix them evenly, and take 3 identical portions;

[0089] S3, the mixture in step S2 is added to each degradation reaction zone in step S1 respectively, the mixture fills the degradation reaction zone, and the top cover is closed;

[0090] S4, start the water pump, control valve and humidity sensor of the water supplement and humidity maintaining system 5, adjust the humidity in each degradation reaction zone to 60%-70%;

[0091] S5, while starting the water supplement and humidity maintaining system 5, start the electric heater 61 and temperature sensor of the heat preservation system 6, adjust the temperature in each degradation reaction zone, and stabilize it at 37℃;

[0092] S6, after the humidity and temperature reach the predetermined conditions, open the switch of the power supply system 4, adjust the applied electric field strength provided by the power supply system 4 to 2V / cm, and start running work;

[0093] S7, after running for 24h, close the power supply system 4, open the bottom plate of the box body 1, take out the degraded tobacco material mixture, and simply wash it with clean water for use.

[0094] Comparative Example 4:

[0095] A method for low-voltage electric field assisted microbial degradation of tobacco cellulose, the steps are as follows:

[0096] S1, the number of electrodes is 4, and the 4 stainless steel electrodes are inserted into the clamping groove 11 of the box body 1;

[0097] S2, take 100g of tobacco material and 20g of microbial agent, mix them evenly, and take 3 identical portions;

[0098] S3, the mixture in step S2 is added to each degradation reaction zone in step S1, and the mixture fills the degradation reaction zone, and the top cover is closed;

[0099] S4, the water supply and moisturizing system 5 is started, the control valve and the humidity sensor are started, and the humidity in each degradation reaction zone is adjusted to 60%-70%;

[0100] S5, while the water supply and moisturizing system 5 is started, the electric heater 61 and the temperature sensor of the heat preservation system 6 are started, the temperature in each degradation reaction zone is adjusted, and is stabilized at 37℃;

[0101] S6, after the humidity and temperature reach the predetermined conditions, the switch of the power supply system 4 is opened, the external electric field strength provided by the power supply system 4 is adjusted to 2V / cm, and the operation is started;

[0102] S7, after 24h of operation, the power supply system 4 is closed, the bottom plate of the box 1 is opened, the degraded tobacco material mixture is taken out, and is simply washed with clean water, and is ready for use.

[0103] The differences between the comparative example 1 and the specific example 1 are as follows:

[0104] The differences between the comparative example 2 and the specific example 1 are as follows: the comparative example 2 does not add 10g of microbial agent.

[0105] The differences between the comparative example 3 and the specific example 1 are as follows: the external electric field strength in the comparative example 3 is 5V / cm.

[0106] The differences between the comparative example 4 and the specific example 1 are as follows: the comparative example 4 adds 20g of microbial agent.

[0107] The test structure is as follows:

[0108] The degradation effects of the specific example 1 and the comparative examples 1-4 on tobacco cellulose are shown in Table 1.

[0109] The degradation effects of the specific example 1 and the comparative examples 1-4 on tobacco cellulose are shown in Table 1. Figures 4-6 It is found by comparison that the specific example 1 can significantly improve the cellulose degradation rate, and save the microbial agent dosage.

[0110] Compared with the simple microbial degradation (comparative example 1) and the simple low-voltage electric field electrolysis (comparative example 2), the electric field enhanced microbial degradation technology (specific example 1 and comparative example 4) significantly improves the cellulose degradation rate, and the electric field enhanced microbial degradation technology (comparative example 3) has too high external voltage, which can cause the microbial electrolysis to die, and significantly inhibit the degradation of cellulose.

[0111] The method of using the tobacco aroma-enhancing and quality-improving device for enhanced cellulose degradation in this embodiment (specific embodiment 1) compared with the electric field-enhanced microbial degradation technology (comparative embodiment 4) is that, under the same external electric field strength, the amount of microbial agent added is only 10%, saving half of the amount of microbial agent used, and achieving the same degradation effect.

[0112] The method of using the tobacco flavor-enhancing and quality-improving device for enhanced cellulose degradation in this embodiment significantly improves the metabolic activity of cellulose-degrading bacteria by applying an external low-voltage electric field. Figure 7 ), oxidative stress response adaptation ( Figure 8 ) and promoted bacterial proliferation ( Figure 9 This method can break the energy barrier of microbial degradation of tobacco cellulose, enhance the tolerance of microorganisms to harmful components in tobacco, improve the degradation of cellulose and hemicellulose that are difficult to biodegrade, and effectively reduce the aroma release temperature of tobacco, thereby effectively enhancing the aroma and quality of tobacco.

[0113] The above description is merely a preferred embodiment of this application and is not intended to limit this application. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A method of using a tobacco flavoring and quality enhancing device that enhances the degradation of cellulose, characterized by, The tobacco flavoring and quality improving device for reinforcing cellulose degradation comprises a box body (1), electrodes, a power supply system (4), a water supplement and moisturizing system (5), and a heat preservation system (6), a plurality of degradation reaction zones are arranged in the box body (1), the tobacco to be treated and microbial inoculants are mixed and put into the degradation reaction zones, the electrodes are arranged in each degradation reaction zone and are electrically connected with the power supply system (4), the water supplement and moisturizing system (5) sprays water into each degradation reaction zone, and the heat preservation system (6) heats and preserves each degradation reaction zone; the electric field strength provided by the power supply system (4) is 2-3.7 V / cm; and the reaction time of the degradation reaction zone is 4 h-48 h. The use method comprises the following steps: S1, according to the quantity of the tobacco to be treated, a proper number of electrodes are inserted into the box body (1), the cathode (2) and the anode (3) of the electrodes are alternately arranged to divide the box body (1) into a plurality of degradation reaction zones, and the cathode (2) and the anode (3) are connected to the power supply; S2, a plurality of portions of tobacco materials with a certain mass and a proper amount of microbial inoculants are weighed, the microbial inoculants are 5%-30% of the mass of the tobacco, and the mixture is uniformly stirred and mixed; S3, the mixture in step S2 is put into each degradation reaction zone, the mixture fills each degradation reaction zone, and the top cover of the box body (1) is closed; S4, the water pump, the control valve, and the humidity sensor of the water supplement and moisturizing system (5) are turned on, and the humidity in each degradation reaction zone is adjusted to 60%-70%; S5, the water pump, the control valve, and the humidity sensor of the water supplement and moisturizing system (5) are turned on, the electric heater (61) and the temperature sensor of the heat preservation system (6) are turned on, the temperature in each degradation reaction zone is adjusted, and is stabilized at 20℃-40℃; S6, after the humidity and the temperature reach the predetermined conditions, the switch of the power supply is turned on, and the external electric field provided by the power supply is adjusted to the predetermined strength; S7, according to the required reaction time, after the reaction is completed, the power supply is turned off, the box body (1) is opened, and the degraded tobacco material mixture is taken out and washed with clean water.

2. A method of using a tobacco flavoring and quality enhancing device for enhanced cellulose degradation as defined in claim 1, wherein: A plurality of symmetrical clamping grooves (11) are arranged in the box body (1), the electrodes are sheet electrodes, and one sheet electrode is inserted into a symmetrical group of clamping grooves (11).

3. A method of using a tobacco flavoring and quality enhancing device for enhanced cellulose degradation as defined in claim 1, wherein: The electrodes are graphite electrodes, platinum electrodes, titanium electrodes, copper electrodes, or stainless steel electrodes.

4. The method of using a device for enhancing the flavor and quality of tobacco by cellulose degradation of claim 1, wherein: The cathode (2) and the anode (3) of the electrodes are arranged at intervals and the interval is 1-20 cm.

5. A method of using a device for enhancing the degradation of cellulose in tobacco for flavor and quality enhancement according to claim 1, wherein: The microbial inoculants are a cellulose and hemicellulose degradation bacteria mixed preparation, and at least comprise one of Bacillus subtilis, Cellulomonas, Vibrio cellulolyticus, Bacteriovorax, Clostridium, Bacteroides succinogenes, and Butyrivibrio fibrisolvens.

6. A method of using a device for enhancing the degradation of cellulose in tobacco for flavor and quality enhancement according to claim 1, wherein: The water supplementing and moisturizing system (5) comprises a water tank, a water conveying pump, a control valve, a metering pump, a water distribution pipe and a humidity sensor, the water distribution pipe connects the water tank, the water conveying pump, the control valve and the metering pump, a water distribution plate (51) is arranged on the side wall of the box body (1), a plurality of water inlet holes (511) and water spraying holes which are communicated with each other are formed in the water distribution plate (51), and the water distribution pipe is connected with the water inlet holes (511).

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