Bacillus deserti strain XT23 microbial inoculum, and preparation method and application thereof

Biological agents were prepared by using fermentation broth of Bacillus desertis strain XT23 through acidification and heat treatment. This solved the problem of reduced activity during the preservation of functional microorganisms, achieving high-efficiency antibacterial effect and stability, and is suitable for the prevention and treatment of aquatic animal diseases.

CN122128180APending Publication Date: 2026-06-02XINGTAI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
XINGTAI UNIV
Filing Date
2026-04-13
Publication Date
2026-06-02

AI Technical Summary

Technical Problem

In existing technologies, the antibacterial activity of functional microorganisms is easily reduced during long-term storage, and chemical disinfectants and antibiotics have problems of residues and drug resistance when controlling Vibrio parahaemolyticus.

Method used

Fermentation broth or supernatant of Bacillus desertis strain XT23, which has undergone acidification and heat treatment, combined with suitable fermentation culture medium and excipients, is prepared into a spray-dried powdered biological agent to enhance the stability and antibacterial activity of live bacteria.

Benefits of technology

It significantly improves the antibacterial activity and storage stability of biological agents, maintaining an antibacterial rate of over 90% and a decrease in viable bacteria count of less than 10%, making it easy to store and transport at room temperature.

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Abstract

The application provides a bacillus desiccabilis strain XT23 microbial agent, a preparation method and application thereof, and belongs to the technical field of biological agents. A biological agent comprises a bacillus desiccabilis strain XT23, a fermentation treatment liquid of the bacillus desiccabilis strain XT23, or supernatant separated from the fermentation treatment liquid of the bacillus desiccabilis strain XT23. The fermentation treatment liquid is obtained by acid treatment and heat treatment of the fermentation liquid of the bacillus desiccabilis strain XT23, which effectively promotes spore formation, inactivates miscellaneous bacteria, significantly improves the stability and storage period of effective live bacteria, maximally retains antibacterial active substances in the fermentation liquid, and makes the inhibition effect of the final biological agent on Vibrio parahaemolyticus equivalent to that of fresh fermentation liquid. Meanwhile, the addition of the auxiliary material has a protective and slow-release effect on the active ingredients, and further enhances the convenience and effect persistence of application.
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