A microfluidic drug testing device integrated with hair pre-treatment

Abstract

The present application relates to the fields of microfluidic device technology, hair treatment, drug detection, and the like, and specifically relates to a microfluidic drug detection device integrated with hair pretreatment, comprising a seat plate, a hair sample processing cavity is arranged on the seat plate, a first sample inlet flow channel, a second sample inlet flow channel and a third sample inlet flow channel, one end of which is installed with a solution storage cover, the first sample inlet flow channel and the second sample inlet flow channel are sequentially connected with the hair sample processing cavity through a three-way two-position diverter and a four-way two-position diverter, the third sample inlet flow channel is connected with the hair sample processing cavity through a four-way two-position diverter, a hair crusher and a pressing device are arranged in the hair sample processing cavity, a waste liquid control mechanism is arranged on the seat plate and connected with the four-way two-position diverter, a mixing channel is further arranged on the seat plate, one end of the mixing channel is connected with the third sample inlet flow channel, and a detection opening is arranged at the other end of the mixing channel. The device realizes the integration of the pretreatment step of the hair sample and real-time detection, and can stably and quickly obtain results.

Description

TECHNICAL FIELD

[0001] The present application relates to the fields of microfluidic device technology, hair treatment, drug detection, and the like, and specifically relates to a microfluidic drug detection device integrated with hair pretreatment. BACKGROUND

[0002] Microfluidic device detection technology is a cross-disciplinary technology derived from microfluidics and microelectronics, aiming to perform efficient and accurate chemical or biological sample operation and detection on a device through miniaturized channels and cavities. The introduction of this technology has brought revolutionary changes to the detection field. As a detection platform, microfluidic devices have many advantages. First, their small size and high integration make the sample and reagent usage greatly reduced, which helps high-throughput experiments. Second, the preparation process of microfluidic devices is relatively simple compared to traditional experimental methods, and the requirements for laboratory conditions are lower, which reduces the complexity and cost of experiments. In addition, microfluidic devices are compatible with a variety of materials, such as PDMS, glass, and ceramic, increasing their flexibility in practical applications. In the field of detection, microfluidic device technology is widely used in the medical field, such as cell analysis, gene detection, and drug screening. Its efficient sample processing capability and small volume reaction chamber make the experimental process faster and more economical.

[0003] During hair growth, drugs and their metabolites in the blood are deposited in the hair, forming traceable marks. Because the deposition of metabolites in hair is relatively uniform, hair detection can provide more stable and reliable results and is not easily affected by short-term fluctuations. Compared to traditional urine or blood detection methods, hair detection has a longer time window, typically up to several months to a year, which can reveal an individual's drug exposure over a relatively long period of time. Therefore, the advantage of hair detection of drugs lies in its ability to provide a stable, long-term record of drug use history.

[0004] In addition, the collection of hair samples is relatively non-invasive, avoiding pain or discomfort, making the detection process more comfortable and acceptable. Hair detection methods can also detect multiple drugs and their metabolites simultaneously, providing more comprehensive information, including drug types, usage frequency, etc. This makes hair detection increasingly widely used in the fields of law enforcement, medicine, and occupational safety. Therefore, hair detection plays a unique role in long-term monitoring of individual drug use behavior, judicial determination, drug abuse prevention and control, and has become a powerful drug detection method.

[0005] Integrating hair pretreatment and drug detection into a microfluidic device greatly reduces the complexity of the experiment and shortens the detection time, allowing rapid and stable detection results to be obtained. SUMMARY

[0006] The application provides a microfluidic drug detection device integrated with hair pretreatment based on a competitive fluorescent immunoassay method, realizes integration of direct sample hair pretreatment steps such as washing, grinding and lysis in the device and real-time detection, and can stably and quickly obtain results.

[0007] The technical scheme of the application is as follows: a microfluidic drug detection device integrated with hair pretreatment, comprising a seat plate, a hair sample processing cavity is arranged on the seat plate, a first sample inlet flow channel, a second sample inlet flow channel and a third sample inlet flow channel are arranged on the seat plate, one end of each of the first sample inlet flow channel and the second sample inlet flow channel is provided with a three-way two-position diverter, the three-way two-position diverter and the four-way two-position diverter are connected with the hair sample processing cavity in sequence, the third sample inlet flow channel is connected with the hair sample processing cavity through a four-way two-position diverter, a hair crusher and a pressing device for sucking air are arranged in the hair sample processing cavity, a waste liquid control mechanism connected with the four-way two-position diverter and provided with an exhaust hole is arranged on the seat plate, and a mixing channel is further arranged on the seat plate, one end of the mixing channel is connected with the third sample inlet flow channel, and a detection opening is arranged at the other end of the mixing channel.

[0008] Further, the solution storage cover comprises a circular truncated cone hollow storage film, the storage film is made of an aluminum foil film, a plastic circular tube for inserting the first sample inlet flow channel is fixed to the bottom surface of the storage film, and a needle rod with a downward pointed tip is fixed to the inner cavity top surface of the storage film.

[0009] Further, the three-way two-position diverter comprises a three-way diverter body extending into the seat plate, three-way diverter flow channels with an angle relationship of 60°, 120° and 180° are formed in the three-way diverter body, and a three-way diverter cap with an arrow is fixed to the upper end of the three-way diverter body; the four-way two-position diverter comprises a four-way diverter body extending into the seat plate, four-way diverter flow channels with an angle relationship of 60°, 30°, 90° and 180° are formed in the four-way diverter body, and a four-way diverter cap with an arrow is fixed to the upper end of the four-way diverter body.

[0010] Further, the four-way two-position diverter and the three-way two-position diverter are connected through a first communication flow channel, the four-way two-position diverter and the hair sample processing cavity are connected through a second communication flow channel, and the four-way two-position diverter and the waste liquid control mechanism are connected through a third communication flow channel; the four-way diverter flow channels of the four-way two-position diverter and the three-way diverter flow channels of the three-way two-position diverter are at the same height and depth with the first sample inlet flow channel, the second sample inlet flow channel, the third sample inlet flow channel, the first communication flow channel, the second communication flow channel and the third communication flow channel.

[0011] Further, the hair crusher comprises a rotating blade arranged at the bottom of the hair sample processing cavity, and the rotating shaft of the rotating blade vertically penetrates the bottom plate and is driven by a motor.

[0012] Further, the presser comprises a first limiting plate installed on the seat plate through a suction cup and located on the upside of the hair treatment cavity, a press plug is vertically arranged on the first limiting plate, the upper end of the press plug is fixed with a press plate, a plurality of springs are arranged between the press plate and the first limiting plate in the circumferential direction, and the lower end of the press plug is fixed with a rubber head used for extending into the hair treatment cavity and in interference fit.

[0013] Further, the waste liquid control mechanism comprises a through hole cavity arranged in the seat plate, the lower end of the through hole cavity is connected with a waste liquid pool, a rubber spring plug is arranged in the waste liquid pool, a second limiting plate is installed on the seat plate through a suction cup, a press rod vertically arranged in the second limiting plate is used for pressing the rubber spring plug and extends into the through hole cavity, the upper end of the press rod is fixed with a press disc, and a plurality of springs are arranged between the press disc and the second limiting plate in the circumferential direction.

[0014] The rubber spring plug comprises a rubber block with a diameter larger than that of the through hole cavity, and the lower end surface of the rubber block is fixed with a plurality of springs in a compressed state; and the waste liquid pool is provided with an exhaust hole extending out of the seat plate upward.

[0015] Further, the seat plate is composed of a base layer and a cover layer, the mixing channel is arranged on the base layer, the mixing channel comprises a collection port connected with the third sample inlet channel at one end, the collection port is connected with a detection port through a first mixing channel, a deposition area and a second mixing channel, and the detection port is connected with a detection opening arranged on the cover layer.

[0016] Further, the first sample inlet channel, the second sample inlet channel, the third sample inlet channel and the hair treatment cavity are arranged on the cover layer, and a filter is arranged in the third sample inlet channel; the cover layer is made of a medical plastic material, the base layer is made of a PDMS material; and the three-way two-position commutator and the four-way two-position commutator are made of a medical plastic material.

[0017] Compared with the prior art, the present application has the following advantages:

[0018] Based on the competitive fluorescence immunoassay technology, the present application combines hair drug testing with microfluidic device technology, and the most prominent feature is that the unique design of the base layer and the cover layer of the device itself and the multiple external elements integrated on the device enables the microfluidic device of the present application to integrate the complex pretreatment steps of the sample hair, such as washing, grinding and filtering, into one device for operation and real-time detection. The successful implementation of the present application greatly reduces the operation difficulty of using hair drug testing and meets the requirement of rapid detection. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1Structure diagram of the integrated hair pretreatment microfluidic drug detection device of the present application;

[0020] Figure 2 Structure diagram of the base layer of the present application;

[0021] Figure 3 Structure diagram of the cover layer of the present application;

[0022] Figure 4 Structure diagram of the solution storage cover of the present application;

[0023] Figure 5 Structure diagram of the three-position two-way commutator of the present application;

[0024] Figure 6 Structure diagram of the four-position two-way commutator of the present application;

[0025] Figure 7 Structure diagram of the presser of the present application;

[0026] Figure 8 Structure diagram of the waste liquid controller of the present application;

[0027] Figure 9 Structure diagram of the rubber spring plug of the present application;

[0028] In the figure: A - seat plate B - mixing channel 1 - base layer 11 - collection port 12 - first mixing flow channel 13 - deposition area 14 - second mixing flow channel 15 - detection port 16 - counterbore 17 - detection port 18 - waste liquid pool 19 - shaft hole 181 - through hole 2 - cover layer 21 - third sample inlet flow channel 22 - second sample inlet flow channel 23 - first sample inlet flow channel 24 - through hole 25 - through hole 26 - hair sample processing cavity 27 - second communication flow channel 28 - through hole cavity 29 - exhaust hole 210 - collection port 211 - detection opening 212 - first communication flow channel 213 - third communication flow channel 261 - shaft hole 262 - flow inlet 3 - solution storage cover 31 - storage film 32 - plastic round tube 311 - needle rod 4 - three-way two-position commutator 41 - three-way commutating cap 411 - pointing arrow 42 - three-way commutating body 421 - commutating flow channel 5 - four-way two-position commutator 51 - four-way commutating cap 511 - pointing arrow 52 - four-way commutating body 521 - commutating flow channel 6 - presser 61 - presser plate 62 - spring 63 - first limit plate 64 - presser plug 65 - rubber head 66 - suction disc 631 - circular through hole 7 - hair crusher 8 - waste liquid control mechanism 81 - presser disc 82 - spring 83 - second limit plate 831 - circular through hole 84 - presser rod 85 - suction disc 9 - rubber spring plug 91 - rubber block 92 - spring 10 - filter. DETAILED DESCRIPTION

[0029] In order to make the above features and advantages of the present application more apparent, specific embodiments are described below in detail with reference to the accompanying drawings, but the present application is not limited thereto.

[0030] REFERENCE Figures 1 to 9

[0031] A microfluidic drug detection device integrated with hair pretreatment includes a seat plate A, the seat plate A is provided with a hair sample processing cavity 26, the seat plate A is provided with a first sample inlet flow channel 23, a second sample inlet flow channel 22 and a third sample inlet flow channel 21, one end of which is installed with a solution storage cover 3, the first sample inlet flow channel 23 and the second sample inlet flow channel 22 are connected with the hair sample processing cavity 26 in sequence through a three-way two-position diverter 4 and a four-way two-position diverter 5, the third sample inlet flow channel 21 is connected with the hair sample processing cavity 26 through the four-way two-position diverter 5, the hair sample processing cavity 26 is provided with a hair crusher 7 and a presser 6 for sucking air, the seat plate A is provided with a waste liquid control mechanism 8 connected with the four-way two-position diverter 5 and having an exhaust hole 29, and the seat plate A is further provided with a mixing channel B, one end of the mixing channel B is connected with the third sample inlet flow channel 21, and the other end of the mixing channel B is provided with a detection opening 211.

[0032] In the embodiment, the solution storage cover 3 includes a circular truncated cone-shaped hollow storage film 31, the storage film 31 is made of a layer of thick aluminum foil film, the bottom surface of the storage film 31 is fixed with a plastic circular tube 32 for inserting the first sample inlet flow channel 23, and the inner cavity top surface of the storage film 31 is fixed with a needle rod 311 with a sharp end downward, so that the needle rod 311 pierces the bottom of the storage film 31 by pressing the storage film 31, and the solution flows into the corresponding sample inlet flow channel. The plastic circular tube 32 is embedded in the port of each sample inlet flow channel in an interference fit.

[0033] In the embodiment, the three-way two-position diverter 4 includes a three-way diverter body 42 extending into the seat plate A, the three-way diverter body 42 is provided with three diverter flow channels 421 communicating with each other and having an angle relationship of 60°, 120° and 180° respectively, and the upper end of the three-way diverter body 42 is fixed with a three-way diverter cap 41 having a pointing arrow 411. The rotation angle of the three-way two-position diverter 4 is determined according to the angle of the arrow on the three-way diverter cap 41, and the flow channels on the seat plate A can be connected by rotating the indicating arrow on the three-way diverter cap 41 to a specified angle through the application of external force.

[0034] In the embodiment, the four-way two-position commutator 5 includes a four-way commutator 52 extending into the seat plate A, the four-way commutator 52 has four commutating flow channels 521 with an angle of 60°, 30°, 90° and 180° respectively, and the upper end of the four-way commutator 52 is fixed with a four-way commutating cap 51 with an arrow 511. The commutation angle of the four-way two-position commutator 5 is determined by the arrow angle of the four-way commutating cap 51, and the flow channels on the seat plate A can be connected by rotating the indicating arrow on the four-way commutating cap 51 to a specified angle by applying an external force.

[0035] In the embodiment, the four-way two-position commutator 5 is connected with the three-way two-position commutator 4 through the first communication flow channel 212, the four-way two-position commutator 5 is connected with the hair sample processing cavity 26 through the second communication flow channel 27, and the four-way two-position commutator 5 is connected with the waste liquid control mechanism 8 through the third communication flow channel 213. The four commutating flow channels 521 of the four-way two-position commutator 5 and the three commutating flow channels 421 of the three-way two-position commutator 4 are at the same height and depth with the first sample inlet flow channel 23, the second sample inlet flow channel 22, the third sample inlet flow channel 21, the first communication flow channel 212, the second communication flow channel 27 and the third communication flow channel 213. The second flow channel 22 is provided with two flow inlets 262 into the hair sample processing cavity 26.

[0036] In the embodiment, the hair crusher 7 includes a rotating blade arranged at the bottom of the hair sample processing cavity 26, the rotating shaft of the rotating blade vertically penetrates the seat plate A and is driven by a motor, and the seat plate A is provided with a shaft hole 261 for the rotating shaft to penetrate the seat plate.

[0037] In the embodiment, the presser 6 includes a first limiting plate 63 detachably mounted on the seat plate A through four suction cups 66 and located on the upper side of the hair processing cavity 26, the first limiting plate 63 is provided with a circular through hole 631 and vertically penetrates the press plug 64, the upper end of the press plug 64 is fixed with a press plate 61 for external pressing, four springs 62 are arranged between the press plate 61 and the first limiting plate 63 in the circumferential direction, and the lower end of the press plug 64 is fixed with a rubber head 65 for extending into the hair processing cavity 26 and interference fit, which helps to improve the sealing performance.

[0038] In the embodiment, the waste liquid control mechanism 8 comprises a through hole cavity 28 arranged in the seat plate A, the lower end of the through hole cavity 28 is connected with a waste liquid pool 18, the waste liquid pool 18 is arranged with a rubber spring plug 9, the seat plate A is installed with a second limiting plate 83 through four suction cups 85, the second limiting plate 83 is arranged with a circular through hole 831 and vertically penetrates a pressing rod 84 which extends into the through hole cavity 28 and is used for pressing the rubber spring plug 9, the upper end of the pressing rod 84 is fixed with a pressing disc 81 which is used for external force pressing, four springs 82 are arranged between the pressing disc 81 and the second limiting plate 83 in the circumferential direction.

[0039] In the embodiment, the rubber spring plug 9 comprises a rubber block 91 with a diameter larger than the through hole cavity, which blocks the entrance of the waste liquid pool 18; the lower end surface of the rubber block 91 is fixed with a plurality of springs 92 in a compressed state, which supports the rubber block 91 and can withstand the weight of the waste liquid, has good sealing performance and will not leak. The lower end of the pressing rod is not in complete contact with the rubber block 91 in the rubber spring plug 9; the waste liquid pool 18 is arranged with an exhaust hole 29 which penetrates the seat plate A upward.

[0040] In the embodiment, the seat plate A is composed of a base layer 1 and a cover plate layer 2, and the base layer and the cover plate layer are packaged by pressing process. The mixing channel is arranged on the base layer 1, the mixing channel B comprises a collection port 11 connected with the third sampling flow channel 21 at one end, the collection port 11 corresponds to the collection port 210 on the third sampling flow channel, the collection port 11 is connected with the detection port 15 through the first mixing flow channel 12, the deposition area 13 and the second mixing flow channel 14, and the detection port 15 is connected with the detection opening 29 arranged on the cover plate layer 2. The deposition area 13 is pre-filled with synthetic antigen of the required detection drug by drying method and is subjected to drying treatment.

[0041] In the embodiment, the base layer 1 is arranged with a counterbore 16 and a counterbore 17 for mounting the three-way two-position diverter 4 and the four-way two-position diverter 5, the cover plate layer 2 is arranged with a through hole 24 and a through hole 25 for the three-way two-position diverter 4 and the four-way two-position diverter 5 to pass through, and the through hole 24 and the through hole 25 correspond to the counterbore 16 and the counterbore 17. The waste liquid pool 18 is arranged on the base layer 1, and the through hole cavity 28 is arranged on the cover plate layer 2 and penetrates the cover plate layer 2.

[0042] In the embodiment, the first sampling flow channel 23, the second sampling flow channel 22, the third sampling flow channel 21 and the hair treatment cavity 26 are arranged on the cover plate layer 2, and the third sampling flow channel 21 is arranged with a filter 10.

[0043] In this embodiment, the cover layer 2 is limited to using medical plastics such as polyvinyl chloride (PVC), polypropylene (PP) or polyethylene (PE); the substrate layer 1 is limited to using PDMS material due to the drying method for drying treatment of synthetic antigens. The materials of the three-position two-way commutator 4 and the four-position two-way commutator 5 are limited to using common medical plastics, and can also use materials such as PMMA.

[0044] Method for use:

[0045] The operation process of pre-treating sample hair on the microfluidic device and the detection process of drug detection can be summarized as:

[0046] (1) Embed the required cleaning solution, hair lysis solution, and drug fluorescent antibody solution in the storage film 31 according to the required proportion, and insert the plastic tube 32 into the corresponding first sample inlet channel 23, second sample inlet channel 22, and third sample inlet channel 21 in an interference fit. Use a coupling to connect the hair crusher 7 in the through hole 19 of the substrate layer 1. Remove the suction disc 66 in the presser 6 adsorbed on the cover layer 2, place the hair sample to be detected into the hair cleaning cavity 26 of the cover layer 2, and reassemble the presser 6 into the hair treatment cavity 26.

[0047] (2) First, rotate the indicator arrow 511 on the four-way commutator cap 51 of the four-position two-way commutator 5 to the vertical direction (arrow upwards), and then rotate it counterclockwise by 30°. Press the presser 6 to expel the air in the hair treatment cavity 26 through the through hole cavity 28 of the cover layer 2, and press the presser 6. Rotate the indicator arrow 411 of the three-way commutator cap 41 of the three-position two-way commutator 7 to the vertical direction (arrow upwards), and at the same time, rotate the indicator arrow 511 on the four-way commutator cap 51 of the four-position two-way commutator 5 clockwise to 30°. Squeeze the first solution storage cover 3 pre-embedded with cleaning solution, and the fine needle 411 in the middle pierces the bottom aluminum foil film. The cleaning solution flows into the device through the first sample inlet channel 23, and at the same time, the presser 6 is loosened. The spring 62 returns to its original length, the presser plug 64 rises upward, generating suction, and the cleaning solution is sucked into the hair treatment cavity 26 according to the connected flow channel to wash the sample hair. When the cleaning solution is completely sucked into the hair treatment cavity 26, immediately rotate the indicator arrow 30° counterclockwise on the four-way commutator cap 51 of the four-position two-way commutator 5. When the spring 62 returns to its original length, press the presser 6 again to generate pressure, and the cleaning solution flows into the through hole cavity 28 of the cover layer 2 according to the connected flow channel. Loosen the presser 6, and the spring 62 returns to its original length. The presser plug 64 rises, generating suction, and the cleaning solution is sucked into the hair treatment cavity 26 again to wash the sample hair. Repeat the pressing to achieve multiple washing of the sample hair.

[0048] (3) After the sample hair is washed, the presser 6 is not released, so that the waste liquid is finally retained in the through-hole cavity 28 of the cover layer 2, and the pressing disc 81 in the waste liquid control mechanism 8 drives the pressing rod 84 to press the rubber block 91 of the rubber spring plug 9 in the waste liquid pool 18 in the base layer 1, drives the spring 92 to stretch and retract downward, and the waste liquid flows from the through-hole cavity 28 of the cover layer into the waste liquid pool 18 in the base layer 1. After the waste liquid completely flows into the waste liquid pool 18, the pressing disc 81 in the waste liquid control mechanism 8 is released, the spring 82 restores the original length, drives the pressing rod 84 to rise, and at the same time, the spring 92 in the rubber spring plug 9 also restores the original length without external force, and the rubber block 91 re-seals the entrance of the waste liquid pool 18. At this time, the spring 62 of the presser 6 is released to restore the original length, and the indicating arrow 30° on the reversing cap 41 of the three-position two-way reversing device 4 is rotated clockwise to connect the other flow channels on the cover layer 2.

[0049] (4) The suction disc 66 at the bottom of the first limiting plate 63 in the presser 6 is pulled out, the cleaned sample hair is clamped from the hair treatment cavity 26 by using tweezers, and the sample hair is subjected to point-by-point spray of ultra-low temperature cold air by using a frozen spray or medical liquid nitrogen. The sample hair is subjected to frozen ice treatment by using the residual moisture of the sample hair. After the frozen ice treatment, the sample hair is re-placed in the hair sample treatment cavity 26, and then the presser 6 is re-assembled in the hair sample treatment cavity 26 by using the suction disc 66 under the first limiting plate 63. The motor is started, and the blade grinds the sample hair after the frozen ice treatment. After the grinding is completed, the motor is turned off, the pressing disc 61 of the presser 6 is pressed and not released, the air in the hair treatment cavity 26 is discharged through the through-hole cavity 28 in the cover layer 2, and the indicating arrow 511 on the reversing cap 51 of the four-position two-way reversing device 5 is rotated clockwise to 30° to restore, and the other flow channels are connected. The second solution storage cover 3 pre-packed with hair lysis solution is squeezed, the inside fine needle 311 pierces the bottom aluminum foil film, the lysis solution flows into the device through the second sample inlet flow channel 22, and at the same time, the presser 6 is released, the spring 62 restores the original length, the pressing plug 64 rises upward, and the suction force is generated. The lysis solution is sucked into the sample hair in the hair treatment cavity 26 according to the flow channel connected by the two reversing devices.

[0050] (5) After the sample hair is lysed, the indicating arrow 511 on the reversing cap 51 of the four-position two-way reversing device 5 is rotated clockwise to 90°, the pressing disc 61 in the presser 6 is pressed and not released, and the lysed sample hair solution is driven to flow through the connected flow channel to the filter block 10 for filtration treatment and flow to the collection port 210 in the cover layer 2. When the sample hair solution is about to reach the collection port 210, the third solution storage cover 3 containing fluorescent antibody is immediately squeezed, the inside fine needle 311 pierces the bottom aluminum foil film, the fluorescent antibody solution flows into the device through the third sample inlet flow channel 21, and is collected with the sample hair solution at the collection port 210 to enter the first mixed flow channel 12 in the base layer 1 for mixing.

[0051] (6) The hair sample solution and the fluorescent antigen solution are mixed through the first mixing channel 12, the mixed solution flows through the deposition area 13, the synthesized antigen is dissolved in the deposition area 13 and mixed with the antigen in the second mixing channel 14, and finally the fluorescence intensity is detected in the detection port 15 to quantitatively determine the concentration of the drug in the hair.

[0052] The above only describes the preferred embodiments of the present application. For those skilled in the art, different forms of integrated hair pretreatment microfluidic drug detection devices can be designed according to the present application without creative labor, and any equivalent changes, modifications, replacements and variations made within the scope of the present application should be covered by the present application.

Claims

1. An integrated hair pre-treatment microfluidic drug testing device comprising a seat plate, characterized in that, The seat plate is provided with a hair sample processing cavity, and the seat plate is provided with a first sample inlet flow channel, a second sample inlet flow channel and a third sample inlet flow channel, one end of each of which is provided with a solution storage cover, the first sample inlet flow channel and the second sample inlet flow channel are sequentially connected with the hair sample processing cavity through a three-way two-position diverter and a four-way two-position diverter, the third sample inlet flow channel is connected with the hair sample processing cavity through the four-way two-position diverter, the hair sample processing cavity is provided with a hair crusher and a pressing device for sucking air, the seat plate is provided with a waste liquid control mechanism connected with the four-way two-position diverter and provided with an exhaust hole, and the seat plate is further provided with a mixing channel, one end of the mixing channel is connected with the third sample inlet flow channel, and the other end of the mixing channel is provided with a detection opening.

2. The integrated hair pre-treatment microfluidic drug testing device of claim 1, wherein, The solution storage cover comprises a hollow storage film in the shape of a circular truncated cone, the storage film is made of an aluminum foil film, the bottom surface of the storage film is fixedly provided with a plastic circular tube for being inserted into the first sample inlet flow channel, and the inner cavity top surface of the storage film is fixedly provided with a needle rod with a downward pointed end.

3. The integrated hair pre-treatment microfluidic drug testing device of claim 1, wherein, The hair crusher comprises a rotating blade arranged at the bottom of the hair sample processing cavity, and the rotating shaft of the rotating blade vertically penetrates the bottom plate and is driven by a motor.

4. The integrated hair pre-treatment microfluidic drug testing device of claim 1, 2, or 3, wherein, The pressing device comprises a first limiting plate mounted on the seat plate through a suction cup and located on the upper side of the hair processing cavity, a pressing plug vertically penetrating the first limiting plate is arranged on the first limiting plate, a pressing plate is fixedly arranged on the upper end of the pressing plug, a plurality of springs are arranged between the pressing plate and the first limiting plate in the circumferential direction, and a rubber head for being inserted into the hair processing cavity and in interference fit is fixedly arranged on the lower end of the pressing plug.

5. The integrated hair pre-treatment microfluidic drug testing device of claim 1, wherein, The rubber spring plug comprises a rubber block with a diameter larger than the through-hole cavity, and a plurality of springs in a compressed state are fixed to the lower end surface of the rubber block; the waste liquid pool is provided with an exhaust hole penetrating out of the seat plate upward.

6. The integrated hair pre-treatment microfluidic drug testing device of claim 1, 2, 3, or 5, wherein, The seat plate is composed of a base layer and a cover layer, the mixing channel is arranged on the base layer, the mixing channel comprises a collection port connected with the third sample inlet channel at one end, the collection port is connected with a detection port through a first mixing channel, a deposition area and a second mixing channel, and the detection port is connected with a detection opening arranged on the cover layer.

7. The integrated hair-pre-treatment microfluidic drug testing device of claim 6, wherein, The first sample inlet channel, the second sample inlet channel, the third sample inlet channel and the hair treatment cavity are arranged on the cover layer, and a filter is arranged in the third sample inlet channel; the cover layer is made of a medical plastic material, the base layer is made of a PDMS material; the three-way two-position commutator and the four-way two-position commutator are made of a medical plastic material.

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