A microfluidic chip and its packaging structure

Abstract

The present invention provides a microfluidic chip and its packaging structure. The microfluidic chip has multiple functional units, and the functional units have cell sorting structures and cell culture chambers. The cell sorting structure sorts single cells from a cell suspension, and the single cells enter the cell culture chamber and are cultured into a cell group. The cell group is screened in the cell culture chamber and then exported. The microfluidic chip provided by the present invention integrates single cell sorting, culture, cell group screening and exporting into one, and the operation is simple and efficient; and the cell sorting structure in the microfluidic chip can improve the capture rate of single cells, avoiding the problem of multi-cell acquisition; in addition, combined with thermal bubble printing technology, the liquid flow of each functional unit can be controlled separately, achieving higher throughput, making the whole process more controllable, convenient and efficient. The packaging structure provided by the present invention is used to package the above-mentioned microfluidic chip, which can realize free switching of samples, convenient perfusion culture and reagent introduction analysis, and is simple and efficient to operate.

Description

Technical Field

[0001] The present invention relates to the fields of microfluidics, cell sorting, cell line development and monoclonal antibody screening, and in particular to a microfluidics chip and its packaging structure. Background Art

[0002] Cells are the basic units of life activities. Research based on the single-cell level can reveal the development laws of life activities at a deeper level. Single-cell sorting is the basis and key to single-cell research. At present, single-cell sorting mainly includes microneedle aspiration method, limiting dilution method, microwell array and microfluidics-based sorting method. However, the current sorting methods face problems such as difficult operation, low efficiency and multi-cell acquisition, which are not conducive to subsequent culture and analysis. In addition, in the application of monoclonal antibody screening and cell line culture, the sorted single cells are mostly placed in well plates for culture. After the cell population is analyzed for potency and phenotype, the cell population with good performance is screened for large-scale culture. The whole process is manpower-intensive, cumbersome to operate, time-consuming and inefficient.

[0003] Therefore, in single-cell research, especially for monoclonal antibody screening and cell line development, there is an urgent need for a simple and efficient method that integrates single-cell sorting, culture, screening, and export. At the same time, developing a packaging structure that is simple to operate, allows for flexible switching of sample introduction, and facilitates perfusion culture and reagent analysis has also become a pressing technical challenge in this field. Summary of the Invention

[0004] In view of the shortcomings of the prior art described above, the present invention provides a microfluidic chip and its packaging structure, wherein the microfluidic chip has a plurality of functional units, wherein the functional units have a cell sorting structure and a cell culture chamber, and the cell sorting structure is used to sort out a single cell from a cell suspension, and the single cell enters the cell culture chamber and is cultured into a cell group, and the cell group is screened in the cell culture chamber and then exported. The microfluidic chip provided by the present invention integrates single cell sorting, culture, cell group screening and exporting into one, thereby improving cell throughput, being simple and efficient to operate, reducing reagent usage and potential cross-contamination risks; and the cell sorting structure in the microfluidic chip can improve the capture rate of single cells, avoiding the problem of multi-cell acquisition; in addition, combined with thermal bubble printing technology, the whole process is more controllable, convenient and efficient. The packaging structure provided by the present invention is used to package the above-mentioned microfluidic chip, which can realize free switching of samples, convenient perfusion culture and reagent introduction analysis, and is simple and efficient to operate.

[0005] To achieve the above-mentioned and other related purposes, the present invention provides a microfluidic chip, comprising an introduction area, a functional area, and an outlet area sequentially arranged along a first direction;

[0006] The introduction area is provided with an introduction port and a micro-pillar array, and the micro-pillar array is distributed on both sides of the introduction port along a second direction perpendicular to the first direction;

[0007] The functional area is provided with a cell flow channel and a plurality of functional units arranged along a first direction, wherein the functional units include a cell sorting structure, a cell culture chamber, a first nozzle, and a second nozzle, wherein the cell sorting structure and the cell culture chamber are arranged side by side in the first direction and are connected through a connecting channel, the first nozzle is connected to the cell sorting structure, and the second nozzle is connected to the cell culture chamber;

[0008] The inlet of the cell sorting structure is connected to the cell flow channel, and the cell suspension flows through the cell flow channel into the cell sorting structure. The cell sorting structure sorts single cells from the cell suspension, and the excess cell suspension is discharged through the first nozzle; the single cells enter the cell culture chamber through the connecting channel and are cultured into cell clusters, and the cell clusters are discharged through the second nozzle;

[0009] The lead-out area is provided with a lead-out port and a micro-column array. The micro-column array is distributed on both sides of the lead-out port along the second direction. The lead-out port is used to discharge waste liquid.

[0010] Optionally, the cell sorting structure is provided with a single cell capture structure, a bypass channel and an outlet channel; the bypass channel includes an inflow section, a bypass section and an outflow section that are sequentially connected, and the flow direction of the inflow section is opposite to that of the outflow section; wherein,

[0011] In the first direction, the inflow section and the connecting channel are located on the same straight line; the outflow section is connected to the outlet channel, and the excess cell suspension flows through the bypass channel and the outlet channel in sequence, and is finally discharged by the first nozzle.

[0012] Optionally, the single cell capturing structure is located between the inflow section and the connecting channel, and the single cell capturing structure is used to capture a single cell in the cell suspension, and the single cell enters the cell culture chamber via the connecting channel.

[0013] Optionally, the single cell capture structure is a single cell capture channel, the inlet of the single cell capture channel is connected to the tube wall of the inflow section, and the outlet of the single cell capture channel is connected to the tube wall of the outflow section.

[0014] Optionally, the inlet size of the single cell capture channel is larger than the outlet size of the single cell capture channel, and the outlet size of the single cell capture channel is set to be smaller than the passing size of the single cell.

[0015] Optionally, the single cell capture structure is a single cell capture chamber, and an opening of the single cell capture chamber is connected to the tube wall of the inflow section.

[0016] Optionally, the first nozzle and the second nozzle are selected from one of a thermal bubble nozzle, a piezoelectric nozzle, a PDMS (polydimethylsiloxane) microvalve, a solenoid valve and a peristaltic pump.

[0017] Optionally, the microfluidic chip includes a flow channel layer, and the inlet area, the outlet area, the cell flow channel, the cell sorting structure, the cell culture chamber, and the connecting channel are all located in the flow channel layer, and the material of the flow channel layer is selected from one of silicon dioxide film, non-photosensitive dry film, and photosensitive dry film.

[0018] Optionally, a micro-column array is provided between the cell culture chamber and the cell flow channel.

[0019] Optionally, the height of the cell culture chamber is set to be greater than or equal to the cell size.

[0020] Optionally, the import area has a plurality of import ports distributed along the second direction; the export area has a plurality of export ports distributed along the second direction; and the functional area has a plurality of functional units arranged along the second direction.

[0021] The present invention also provides a packaging structure, comprising:

[0022] A base having a first surface and a second surface disposed opposite to each other, and a liquid inlet and a liquid outlet penetrating the first surface and the second surface;

[0023] a cover plate, located on the first surface of the base, having a sample inlet slot and a sample outlet slot on the cover plate, wherein the sample inlet slot is correspondingly arranged with the liquid inlet, and the sample outlet slot is correspondingly arranged with the liquid outlet;

[0024] a chip located on the second surface of the base, wherein the chip is any one of the microfluidic chips described above, and the inlet of the microfluidic chip is correspondingly arranged with the liquid inlet, and the outlet of the microfluidic chip is correspondingly arranged with the liquid outlet;

[0025] A circuit board is electrically connected to the chip.

[0026] The microfluidic chip and its packaging structure provided by the present invention have at least the following beneficial effects:

[0027] The microfluidic chip provided by the present invention integrates single-cell sorting, culture, cell population screening, and export, improving cell throughput, simplifying and efficiently operating, reducing reagent usage and potential cross-contamination risks. Furthermore, the cell sorting structure in the microfluidic chip can improve the capture rate of single cells, avoiding the problem of multi-cell acquisition. Furthermore, combined with thermal bubble printing technology, the entire process is more controllable, convenient, and efficient. The packaging structure provided by the present invention is used to package the above-mentioned microfluidic chip, enabling free switching of samples, convenient perfusion culture, and reagent introduction analysis, with simple and efficient operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 Shown is a schematic structural diagram of the microfluidic chip provided in Example 1.

[0029] Figure 2 Shown is a schematic structural diagram of a functional unit with a single cell capture chamber in Example 1.

[0030] Figure 3 Shown is a schematic structural diagram of a functional unit with a single cell capture channel in Example 1.

[0031] Figure 4 Shown is a schematic diagram of the working principle of the microfluidic chip provided in Example 1.

[0032] Figures 5a-5b Shown is a structural schematic diagram of the packaging structure provided in Example 2.

[0033] Component number description

[0034] 1 Cell flow channel 32 outlet

[0035] 2 Functional Unit 4 Micropillar Array

[0036] 21 Cell Sorting Structure 5 Single Cell

[0037] 22 Cell Culture Chamber 100 Base

[0038] 23 First nozzle 200 cover plate

[0039] 24 Second nozzle 300 chip

[0040] 25 Connection channel 400 circuit board

[0041] 211 Single cell capture structure 110 First surface

[0042] 212 bypass channel 120 second surface

[0043] 213 Export channel 101 Liquid inlet

[0044] 2121 Inflow section 102 liquid outlet

[0045] 2122 bypass section 201 injection slot

[0046] 2123 Outflow section 202 Sample trough

[0047] 31 Import port DETAILED DESCRIPTION

[0048] The following describes the embodiments of the present invention through specific examples. Those skilled in the art will readily understand the other advantages and benefits of the present invention from the disclosure herein. The present invention may also be implemented or applied through various other specific embodiments, and the details in this specification may be modified or altered based on different viewpoints and applications without departing from the spirit of the present invention.

[0049] It should be noted that the illustrations provided in this embodiment only illustrate the basic concept of the present invention in a schematic manner. Although the illustrations only show components related to the present invention and are not drawn according to the number, shape and size of components in actual implementation, the form, quantity, positional relationship and proportion of each component in actual implementation can be changed at will under the premise of realizing the technical solution of this party, and the component layout form may also be more complicated.

[0050] Example 1

[0051] This embodiment provides a microfluidic chip, such as Figure 1 As shown, including along the first direction ( Figure 1 The functional area is provided with a cell flow channel 1 and a plurality of cells along the first direction ( Figure 1 The functional units 2 are arranged in the X-axis direction as shown.

[0052] like Figure 1 As shown, the introduction area is provided with an introduction port 31 and a micro-pillar array 4, and the micro-pillar array 4 is arranged along a second direction perpendicular to the first direction ( Figure 1 The micropillar array 4 (shown in the Y-axis direction) is distributed on both sides of the inlet 31 to intercept impurities and multi-cell clusters, preventing them from clogging subsequent channels. A cell suspension or culture fluid enters the microfluidic chip through the inlet 31, flows sequentially through the micropillar array 4 and the cell flow channel 1, and then enters the multiple functional units 2. As an example, the micropillar array 4 includes a plurality of spaced-apart micropillars, which can be made of silicon or silicon dioxide.

[0053] like Figure 1 As shown, the lead-out area is provided with a lead-out port 32 and a micro-pillar array 4, and the micro-pillar array 4 is also arranged along the second direction ( Figure 1The waste liquid flowing out of the multiple functional units 2 passes through the cell flow channel 1 and the micro-pillar array 4 and is discharged from the outlet 32.

[0054] like Figure 2 As shown, the functional unit 2 includes a cell sorting structure 21, a cell culture chamber 22, a first nozzle 23 and a second nozzle 24, wherein the cell sorting structure 21 and the cell culture chamber 22 are arranged in a first direction ( Figure 2 The first nozzle 23 is connected to the cell sorting structure 21, and the second nozzle 24 is connected to the cell culture chamber 22.

[0055] like Figure 2 As shown, the inlet of the cell sorting structure 21 is connected to the cell flow channel 1, and the cell sorting structure 21 is provided with a single cell capturing structure 211, a bypass channel 212 and a lead-out channel 213.

[0056] like Figure 2 As shown, the bypass channel 212 includes an inflow section 2121, a bypass section 2122 and an outflow section 2123 that are connected in sequence, and the flow directions of the inflow section 2121 and the outflow section 2123 are opposite. Figure 2 In the X-axis direction, the inflow section 2121 and the connecting channel 25 are located on the same straight line; the outflow section 2123 is connected to the outlet channel 213.

[0057] like Figure 2 As shown, the single-cell capture structure 211 is located between the bypass channel 212 and the connecting channel 25. The single-cell capture structure 211 is used to capture a single cell 5 in a cell suspension, which then enters the cell culture chamber 22 via the connecting channel 25. The flow resistance of the single-cell capture structure 211 is configured to be less than the flow resistance of the bypass section 2122, thereby ensuring that cells passing through the structure preferentially flow toward the single-cell capture structure 211 and achieve single-cell capture. In this embodiment, the single-cell capture structure 211 is configured to accommodate only a single cell 5. After the cell is captured, the flow resistance of the single-cell capture structure 211 increases, and subsequent cells preferentially flow toward the bypass channel 212, ensuring the accuracy of single-cell interception in the single-cell capture structure 211.

[0058] Specifically, the flow resistance of the channel can be controlled by adjusting the channel size, channel length, channel pattern, etc. For example, with the same channel size and pattern, the longer the channel length, the greater the flow resistance. As an example, the bypass section 2122 bends back and forth at least once to increase the flow resistance. In this embodiment, the bypass section 2122 is U-shaped.

[0059] In this embodiment, if Figure 2As shown, the single cell capture structure 211 is a single cell capture chamber, and the opening of the single cell capture chamber is connected to the tube wall of the inflow section 2121.

[0060] In other optional embodiments, such as Figure 3 As shown, the single-cell capture structure 211 is a single-cell capture channel. This single-cell capture channel is generally linear, with its inlet connected to the wall of the inflow section 2121, and its outlet connected to the wall of the outflow section 2123. The inlet of the single-cell capture channel is larger than its outlet, and the outlet is smaller than the passage size of a single cell, preventing a single cell 5 from entering the channel and being discharged through its outlet.

[0061] like Figure 2 As shown, the inlet of the outlet channel 213 is connected to the outflow section 2123 of the bypass channel, and the outlet is connected to the first nozzle 23 . The remaining cell suspension after sorting flows through the outlet channel 213 and is discharged by the first nozzle 23 .

[0062] like Figure 2 As shown, the cell culture chamber 22 is connected to the cell sorting structure 21 via a connecting channel 25, and the cell culture chamber 22 is also connected to the second nozzle 24. The sorted single cells 5 enter the cell culture chamber 22 through the connecting channel 25, where they are cultured into a cell cluster, which is then discharged from the second nozzle 24. As an example, a micro-pillar array 4 is provided between the cell culture chamber 22 and the cell flow channel 1 to prevent cell loss during the culture process; the height of the cell culture chamber 22 is set to be greater than or equal to the cell size. Preferably, the height of the cell culture chamber 22 is set to accommodate only a single layer of cells to ensure the single layer of cells is arranged during the cell culture process, which facilitates subsequent cell counting and fluorescence analysis.

[0063] As an example, the first nozzle 23 and the second nozzle 24 can be selected from a group consisting of a thermal bubble nozzle, a piezoelectric nozzle, a PDMS (polydimethylsiloxane) microvalve, a solenoid valve, and a peristaltic pump, with a thermal bubble nozzle being preferred. A heating film is integrated at the bottom of the channel, and the instantaneous high temperature of the heating film is used to vaporize the liquid above the thermal bubble nozzle, thereby generating bubbles that drive the liquid flow and eject it from the thermal bubble nozzle. Subsequently, the subsequent liquid is replenished under the action of capillary force, thereby providing power for the continuous flow of the liquid. Each functional unit 2 is provided with a separate first nozzle 23 and second nozzle 24, so that the liquid flow of each functional unit 2 can be individually controlled, and the overall flow resistance will not increase significantly with the increase in the number of functional units, thereby making the number of functional units unlimited or less limited, achieving a higher throughput. In addition, the use of a thermal bubble nozzle as a single cell extraction component can not only achieve rapid and high-throughput extraction of single cells, but also cause less damage to the cells, and can achieve gentle extraction of single cells.

[0064] As an example, the microfluidic chip provided in this embodiment includes a flow channel layer, wherein the inlet region, outlet region, cell flow channel 1, cell sorting structure 21, cell culture chamber 22, and connecting channel 25 are all located in the flow channel layer. In this embodiment, the material of the flow channel layer can be selected from one of silica film, non-photosensitive dry film, and photosensitive dry film. Preferably, the flow channel layer is made of silica film or non-photosensitive dry film material to avoid the influence of traditional dry film autofluorescence on cell sorting. Among them, the non-photosensitive dry film can be made of spin-on glass (SOG, a polysilane spin-coated material), non-photosensitive epoxy resin, non-photosensitive polyimide, and other materials.

[0065] The working principle of the microfluidic chip provided in this embodiment is as follows:

[0066] Combine Figure 1 and Figure 4 As shown, during the cell sorting process, a cell suspension is added to the inlet 31, and a syringe pump is used to extract the cell suspension at the outlet 32, so that the cell suspension flows through the cell flow channel 1 and, driven by the first nozzle 23, single cells are captured in the cell sorting structure 21. Specifically, since the flow resistance of the single cell capture structure 211 is smaller than the flow resistance of the bypass section 2122, the cells flowing through the single cell capture structure 211 preferentially flow to the single cell capture structure 211, thereby achieving single cell 5 capture; the excess cell suspension passes through the outlet channel 213 and is finally discharged by the first nozzle 23. It should be noted that Figure 4 The single cell capture structure 211 is described as a single cell capture chamber. When the single cell capture structure 211 is a single cell capture channel, refer to Figure 3 shown.

[0067] Next, cell culture is performed. The second nozzle 24 is actuated to release the captured single cell 5 and introduce it into the cell culture chamber 22. Cell culture fluid is added to the inlet 31, and a syringe pump is used to draw the cell culture fluid through the outlet 32, allowing it to diffuse into the cell culture chamber 22. After a period of culture in the cell culture chamber 22, the single cell 5 becomes a cell cluster.

[0068] Finally, cell population screening is performed. Immunomagnetic beads and fluorescent antibodies are introduced into the cell culture chamber 22. Fluorescence microscopy is used to characterize the titer of cell secreted proteins. Cell populations with high titers are selected from the chip and directed into the well plate through the second nozzle 24.

[0069] Example 2

[0070] This embodiment provides a packaging structure, such as Figures 5a-5b As shown, it includes a base 100 , a cover 200 , a chip 300 and a circuit board 400 .

[0071] As an example, the base 100 has a first surface 110 and a second surface 120 opposite to each other, and is provided with a liquid inlet 101 and a liquid outlet 102 penetrating the first surface 110 and the second surface 120. In this embodiment, the base 100 can be made of ceramic, stainless steel, plastic, or other materials.

[0072] As an example, the cover plate 200 is positioned on the first surface 110 of the base, and the cover plate 200 is bonded to the base 100 by thermosetting glue. The cover plate 200 has an inlet groove 201 and an outlet groove 202, wherein the inlet groove 201 is provided corresponding to the liquid inlet 101, and the outlet groove 202 is provided corresponding to the liquid outlet 102. The inlet groove 201 is used to introduce a cell suspension or cell culture fluid into the chip 300. After flowing through the inlet groove 201 and the liquid inlet 101, the cell suspension or cell culture fluid enters the chip 300. The outlet groove 202 is used to remove the cell suspension or cell culture fluid from the chip 300. After flowing through the liquid outlet 102 and the outlet groove 202 in sequence, the cell suspension or cell culture fluid is removed from the package structure. In this embodiment, the sample inlet 201 and the sample outlet 202 use Luer interfaces that are easy to plug and unplug, and can be used directly as sample inlet and outlet slots, or can be connected to other sample pipelines to achieve flexible switching and introduction of multiple samples.

[0073] As an example, chip 300 is located on the second surface 120 of the base, and chip 300 is the microfluidic chip provided in Example 1. Its specific structure can be referred to the description of Example 1 and will not be repeated here. As an example, the inlet 31 in chip 300 is configured to correspond to the liquid inlet 101, and the outlet 32 ​​in chip 300 is configured to correspond to the liquid outlet 102.

[0074] In this embodiment, the second surface 120 of the base is further provided with a glue dispensing groove and a glue overflow prevention dam (not shown) for bonding the chip 300. Specifically, an appropriate amount of thermosetting glue is evenly dispensed into the glue dispensing groove, and the chip 300 and the second surface 120 of the base are aligned and bonded. After bonding, a heat curing process is performed to fully cure the glue and prevent the chip 300 from shifting during the subsequent packaging process.

[0075] As an example, circuit board 400 is electrically connected to chip 300 and serves to connect chip 300 to an external control system. In this embodiment, circuit board 400 is a flexible circuit board. Double-sided tape or glue can be used to bond the flexible circuit board to base 100, to which chip 300 is attached. Wire bonding or thermal compression bonding of pads are then used to electrically connect the chip 300 to the flexible circuit board. The flexible circuit board is then connected to an external control system to control chip operation. After wire bonding or thermal compression bonding of pads, the wires are protected with UV thermosetting glue, and UV pre-curing and thermal curing are performed.

[0076] As an example, in the packaging structure provided in this embodiment, all packaging materials involved are biocompatible and biosterilizable.

[0077] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the present invention. Anyone skilled in the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by one of ordinary skill in the art without departing from the spirit and technical principles disclosed herein are intended to be covered by the claims of the present invention.

Claims

1. A microfluidic chip, characterized in that: It includes a lead-in area, a functional area and a lead-out area sequentially arranged along a first direction; The introduction area is provided with an introduction port and a micro-pillar array, and the micro-pillar array is distributed on both sides of the introduction port along a second direction perpendicular to the first direction; The functional area is provided with a cell flow channel and a plurality of functional units arranged along a first direction, wherein the functional units include a cell sorting structure, a cell culture chamber, a first nozzle, and a second nozzle, wherein the cell sorting structure and the cell culture chamber are arranged side by side in the first direction and are connected via a connecting channel, the first nozzle is connected to the cell sorting structure, and the second nozzle is connected to the cell culture chamber; a cell suspension or culture fluid enters the microfluidic chip through an inlet, flows through a microcolumn array and the cell flow channel in sequence, and then enters the plurality of functional units; The inlet of the cell sorting structure is connected to the cell flow channel, and the cell suspension flows through the cell flow channel into the cell sorting structure. The cell sorting structure sorts single cells from the cell suspension, and the excess cell suspension is discharged through the first nozzle; the waste liquid flowing out of the multiple functional units passes through the cell flow channel and the micro-column array and is discharged from the outlet; the single cell enters the cell culture chamber through the connecting channel and is cultured into a cell group, and the cell group is discharged through the second nozzle; The cell sorting structure is provided with a single cell capture structure, a bypass channel and an outlet channel; the bypass channel includes an inflow section, a bypass section and an outflow section that are sequentially connected, and the flow direction of the inflow section is opposite to that of the outflow section; wherein, In the first direction, the inflow section and the connecting channel are located on the same straight line; the outflow section is connected to the outlet channel, and the excess cell suspension flows through the bypass channel and the outlet channel in sequence, and is finally discharged by the first nozzle; The single cell capture structure is located between the inflow section and the connecting channel, and is used to capture a single cell in the cell suspension, and the single cell enters the cell culture chamber through the connecting channel; A micro-column array is provided between the cell culture chamber and the cell flow channel; The lead-out area is provided with a lead-out port and a micro-pillar array, the micro-pillar array is distributed on both sides of the lead-out port along the second direction, and the lead-out port is used to discharge waste liquid; The import area has a plurality of import ports distributed along the second direction; the export area has a plurality of export ports distributed along the second direction; and the functional area has a plurality of functional units arranged along the second direction.

2. The microfluidic chip according to claim 1, characterized in that The single cell capture structure is a single cell capture channel, the inlet of the single cell capture channel is connected to the tube wall of the inflow section, and the outlet of the single cell capture channel is connected to the tube wall of the outflow section.

3. The microfluidic chip according to claim 2, characterized in that: The inlet size of the single cell capture channel is larger than the outlet size of the single cell capture channel, and the outlet size of the single cell capture channel is set to be smaller than the passing size of the single cell.

4. The microfluidic chip according to claim 1, characterized in that The single cell capturing structure is a single cell capturing chamber, and the opening of the single cell capturing chamber is connected to the tube wall of the inflow section.

5. The microfluidic chip according to claim 1, characterized in that The first nozzle and the second nozzle are selected from one of a thermal bubble nozzle, a piezoelectric nozzle, a PDMS (polydimethylsiloxane) microvalve, a solenoid valve and a peristaltic pump.

6. The microfluidic chip according to claim 1, characterized in that The microfluidic chip includes a flow channel layer, and the inlet area, the outlet area, the cell flow channel, the cell sorting structure, the cell culture chamber, and the connecting channel are all located in the flow channel layer. The material of the flow channel layer is selected from one of silicon dioxide film, non-photosensitive dry film, and photosensitive dry film.

7. The microfluidic chip according to claim 1, characterized in that The height of the cell culture chamber is set to be greater than or equal to the cell size.

8. A packaging structure, characterized in that: include: A base having a first surface and a second surface disposed opposite to each other, and a liquid inlet and a liquid outlet penetrating the first surface and the second surface; a cover plate, located on the first surface of the base, having a sample inlet slot and a sample outlet slot on the cover plate, wherein the sample inlet slot is correspondingly arranged with the liquid inlet, and the sample outlet slot is correspondingly arranged with the liquid outlet; a chip located on the second surface of the base, wherein the chip is the microfluidic chip according to any one of claims 1 to 7, wherein the inlet of the microfluidic chip is correspondingly arranged with the liquid inlet, and the outlet of the microfluidic chip is correspondingly arranged with the liquid outlet; A circuit board is electrically connected to the chip.

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