A microfluidic chip and its packaging structure

Abstract

The present invention provides a microfluidic chip and its packaging structure. The microfluidic chip has multiple functional units, and the functional units have a cell sorting structure and a cell culture chamber. The cell sorting structure sorts single cells from a cell suspension, and the single cells enter the cell culture chamber and are cultured into a cell group. The cell group is screened in the cell culture chamber and then exported. The microfluidic chip provided by the present invention integrates single cell sorting, culture, cell group screening and exporting into one, and the operation is simple and efficient, which reduces the amount of reagents and the potential risk of cross-contamination; and the cell sorting structure can improve the capture rate of single cells and avoid the problem of multi-cell acquisition; in addition, combined with thermal bubble printing technology, it helps to achieve higher throughput, making the whole process more controllable, convenient and efficient. The packaging structure provided by the present invention is used to package the above-mentioned microfluidic chip, which can realize free switching of samples, convenient perfusion culture and reagent introduction analysis, and is simple and efficient to operate.

Description

Technical Field

[0001] The present invention relates to the fields of microfluidics, cell sorting, cell line development and monoclonal antibody screening, and in particular to a microfluidics chip and its packaging structure. Background Art

[0002] Cells are the basic units of life activities. Research based on the single-cell level can reveal the development laws of life activities at a deeper level. Single-cell sorting is the basis and key to single-cell research. At present, single-cell sorting mainly includes microneedle aspiration method, limiting dilution method, microwell array and microfluidics-based sorting method. However, the current sorting methods face problems such as difficult operation, low efficiency and multi-cell acquisition, which are not conducive to subsequent culture and analysis. In addition, in the application of monoclonal antibody screening and cell line culture, the sorted single cells are mostly placed in well plates for culture. After the cell population is analyzed for potency and phenotype, the cell population with good performance is screened for large-scale culture. The whole process is manpower-intensive, cumbersome to operate, time-consuming and inefficient.

[0003] Therefore, in single-cell research, especially for monoclonal antibody screening and cell line development, there is an urgent need for a simple and efficient method that integrates single-cell sorting, culture, screening, and export. At the same time, developing a packaging structure that is simple to operate, allows for flexible switching of sample introduction, and facilitates perfusion culture and reagent analysis has also become a pressing technical challenge in this field. Summary of the Invention

[0004] In view of the shortcomings of the prior art described above, the present invention provides a microfluidic chip and its packaging structure, wherein the microfluidic chip has a plurality of functional units, wherein the functional units have a cell sorting structure and a cell culture chamber, and the cell sorting structure is used to sort out a single cell from a cell suspension, and the single cell enters the cell culture chamber and is cultured into a cell group, and the cell group is screened in the cell culture chamber and then exported. The microfluidic chip provided by the present invention integrates single cell sorting, culture, cell group screening and exporting into one, thereby improving cell throughput, being simple and efficient to operate, reducing reagent usage and potential cross-contamination risks; and the cell sorting structure in the microfluidic chip can improve the capture rate of single cells, avoiding the problem of multi-cell acquisition; in addition, combined with thermal bubble printing technology, the whole process is more controllable, convenient and efficient. The packaging structure provided by the present invention is used to package the above-mentioned microfluidic chip, which can realize free switching of samples, convenient perfusion culture and reagent introduction analysis, and is simple and efficient to operate.

[0005] To achieve the above objectives and other related objectives, the present invention provides a microfluidic chip, comprising a first injection port, a second injection port, and a functional area, wherein the first injection port and the second injection port both extend along a first direction, and the functional area is arranged between the first injection port and the second injection port;

[0006] The functional area includes a plurality of functional units arranged along a first direction, wherein the functional units include a cell flow channel, a cell sorting structure, a cell culture chamber, a first nozzle, and a second nozzle, wherein the cell flow channel, the cell sorting structure, and the cell culture chamber are arranged in sequence along a second direction perpendicular to the first direction, and the cell flow channel is connected to the first injection port, and the cell culture chamber is connected to the second injection port;

[0007] The cell flow channel comprises a first cell flow channel and a second cell flow channel arranged in parallel in a first direction, wherein one end of the first cell flow channel is connected to the first injection port, and the other end is connected to the inlet of the cell sorting structure; one end of the second cell flow channel is connected to the outlet of the cell sorting structure, and the other end is connected to the first nozzle;

[0008] The cell culture chamber is connected to the cell sorting structure and the second nozzle. The cell sorting structure sorts single cells from the cell suspension. The single cells enter the cell culture chamber and are cultured into cell groups. The cell groups are then guided out by the second nozzle.

[0009] Optionally, a micro-column array is provided between the cell culture chamber and the second sample inlet.

[0010] Optionally, the cell sorting structure is provided with a single cell capture structure, a bypass channel and a single cell export channel, the single cell capture structure is used to capture a single cell in the cell suspension, the single cell enters the cell culture chamber via the single cell export channel, and the remaining cell suspension enters the second cell flow channel via the bypass channel.

[0011] Optionally, the bypass channel includes an inflow section, a bypass section and an outflow section that are sequentially connected, and the flow direction of the inflow section is opposite to that of the outflow section.

[0012] Optionally, the single cell capture structure is a single cell capture channel, the inlet of the single cell capture channel is connected to the tube wall of the inflow section, and the outlet of the single cell capture channel is connected to the tube wall of the outflow section.

[0013] Optionally, the inlet size of the single cell capture channel is larger than the outlet size of the single cell capture channel, and the outlet size of the single cell capture channel is set to be smaller than the passing size of the single cell.

[0014] Optionally, the single cell capture structure is a single cell capture chamber, and an opening of the single cell capture chamber is connected to the tube wall of the inflow section.

[0015] Optionally, the first nozzle and the second nozzle are selected from one of a thermal bubble nozzle, a piezoelectric nozzle, a PDMS (polydimethylsiloxane) microvalve, a solenoid valve and a peristaltic pump.

[0016] Optionally, the microfluidic chip includes a flow channel layer, and the first sample inlet, the second sample inlet, the cell flow channel, the cell sorting structure, and the cell culture chamber are all located in the flow channel layer, and the material of the flow channel layer is selected from one of silicon dioxide film, non-photosensitive dry film, and photosensitive dry film.

[0017] Optionally, the height of the cell culture chamber is set to be greater than or equal to the cell size.

[0018] Optionally, the microfluidic chip is provided with a plurality of the first injection ports, the second injection ports and the functional areas in the second direction, and adjacent first injection ports and second injection ports are separated from each other.

[0019] The present invention also provides a packaging structure, comprising:

[0020] A base having a first surface and a second surface opposite to each other, and a first liquid inlet and a second liquid inlet penetrating the first surface and the second surface;

[0021] A base, located on the first surface of the base, having a first sample injection slot and a second sample injection slot, wherein the first sample injection slot is correspondingly arranged with the first liquid inlet, and the second sample injection slot is correspondingly arranged with the second liquid inlet;

[0022] A chip located on the second surface of the base, wherein the chip is the microfluidic chip described in any one of the above items, and the first sample inlet in the microfluidic chip is correspondingly arranged with the first liquid inlet, and the second sample inlet in the microfluidic chip is correspondingly arranged with the second liquid inlet;

[0023] A circuit board is electrically connected to the chip.

[0024] The microfluidic chip and its packaging structure provided by the present invention have at least the following beneficial effects:

[0025] The microfluidic chip provided by the present invention integrates single-cell sorting, culture, cell population screening, and export, improving cell throughput, simplifying and efficiently operating, reducing reagent usage and potential cross-contamination risks. Furthermore, the cell sorting structure in the microfluidic chip can improve the capture rate of single cells, avoiding the problem of multi-cell acquisition. Furthermore, combined with thermal bubble printing technology, the entire process is more controllable, convenient, and efficient. The packaging structure provided by the present invention is used to package the above-mentioned microfluidic chip, enabling free switching of samples, convenient perfusion culture, and reagent introduction analysis, with simple and efficient operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 Shown is a schematic structural diagram of the microfluidic chip provided in Example 1.

[0027] Figure 2 Shown is a schematic structural diagram of the cell sorting structure with a single cell capture chamber in Example 1.

[0028] Figure 3 Shown is a schematic structural diagram of the cell sorting structure with a single cell capture channel in Example 1.

[0029] Figures 4a-4b Shown is a structural schematic diagram of the packaging structure provided in Example 2.

[0030] Figure 5 Shown is a structural schematic diagram of the base in the packaging structure provided in the second embodiment.

[0031] Component number description

[0032] 11 First injection port 24 First nozzle

[0033] 12 Second injection port 25 Second nozzle

[0034] 2 Functional Unit 3 Single Cell

[0035] 21 Cell flow channel 4 Microcolumn array

[0036] 211 first cell flow channel 100 base

[0037] 212 second cell flow channel 200 base

[0038] 22 Cell Sorting Structure 300 Chip

[0039] 221 Single Cell Capture Structure 400 Circuit Board

[0040] 222 bypass channel 110 first surface

[0041] 2221 Inflow section 120 Second surface

[0042] 2222 Detour section 101 First liquid inlet

[0043] 2223 Outflow section 102 Second liquid inlet

[0044] 223 Single cell export channel 201 First injection slot

[0045] 23 Cell culture chamber 202 Second injection slot DETAILED DESCRIPTION

[0046] The following describes the embodiments of the present invention through specific examples. Those skilled in the art will readily understand the other advantages and benefits of the present invention from the disclosure herein. The present invention may also be implemented or applied through various other specific embodiments, and the details in this specification may be modified or altered based on different viewpoints and applications without departing from the spirit of the present invention.

[0047] It should be noted that the illustrations provided in this embodiment only illustrate the basic concept of the present invention in a schematic manner. Although the illustrations only show components related to the present invention and are not drawn according to the number, shape and size of components in actual implementation, the form, quantity, positional relationship and proportion of each component in actual implementation can be changed at will under the premise of realizing the technical solution of this party, and the component layout form may also be more complicated.

[0048] Example 1

[0049] This embodiment provides a microfluidic chip, such as Figure 1 As shown, it includes a first injection port 11, a second injection port 12 and a functional area, the functional area is arranged between the first injection port 11 and the second injection port 12, and the functional area includes a plurality of Figure 1 The functional units 2 are arranged in the X-axis direction as shown.

[0050] like Figure 1 As shown, the first injection port 11 and the second injection port 12 are both along the first direction ( Figure 1 The first injection port 11 is used to introduce a cell suspension into the plurality of functional units 2 , and the second injection port 12 is used to introduce a cell culture fluid into the plurality of functional units 2 .

[0051] like Figure 1 As shown, the functional unit 2 includes a cell flow channel 21, a cell sorting structure 22, a cell culture chamber 23, a first nozzle 24 and a second nozzle 25, wherein the cell flow channel 21, the cell sorting structure 22, and the cell culture chamber 23 are arranged along a second direction perpendicular to the first direction ( Figure 1 The cell flow channel 21 is connected to the first sample inlet 11 , and the cell culture chamber 23 is connected to the second sample inlet 12 .

[0052] like Figure 1 As shown, the cell flow channel 21 includes a first direction ( Figure 1The first cell flow channel 211 and the second cell flow channel 212 are arranged side by side (in the X-axis direction shown). One end of the first cell flow channel 211 is connected to the first sample inlet 11, and the other end is connected to the inlet of the cell sorting structure 22; one end of the second cell flow channel 212 is connected to the outlet of the cell sorting structure 22, and the other end is connected to the first nozzle 24. During the cell sorting process, the cell suspension enters the cell sorting structure 22 through the first cell flow channel 211, and single cells are captured in the cell sorting structure 22. The excess cell suspension flows out of the cell sorting structure 22, flows through the second cell flow channel 212, and is finally discharged by the first nozzle 24.

[0053] like Figure 2 As shown, the cell sorting structure 22 is provided with a single cell capturing structure 221 , a bypass channel 222 and a single cell outlet channel 223 .

[0054] like Figure 2 As shown, the bypass channel 222 includes an inflow section 2221, a bypass section 2222 and an outflow section 2223 that are sequentially connected, and the flow directions of the inflow section 2221 and the outflow section 2223 are opposite. Figure 2 In the Y-axis direction shown in the figure, the inflow section 2221 and the single cell outlet channel 223 are located on the same straight line.

[0055] like Figure 2 As shown, the single-cell capture structure 221 is located between the first cell flow channel 211 and the second cell flow channel 212. The single-cell capture structure 221 is used to capture a single cell 3 in a cell suspension, which enters the cell culture chamber 23 via the single-cell outlet channel 223. The flow resistance of the single-cell capture structure 221 is set to be less than the flow resistance of the bypass section 2222, thereby ensuring that cells passing through the single-cell capture structure 221 preferentially flow toward the single-cell capture structure 221 and achieve single-cell capture. In this embodiment, the single-cell capture structure 221 is configured to accommodate only a single cell 3. After the cell is captured, the flow resistance of the single-cell capture structure 221 increases, and subsequent cells preferentially flow toward the bypass channel 222, ensuring the accuracy of single-cell interception in the single-cell capture structure 221.

[0056] Specifically, the flow resistance of the channel can be controlled by adjusting the channel size, channel length, channel pattern, etc. For example, with the same channel size and pattern, the longer the channel length, the greater the flow resistance. As an example, the bypass section 2222 bends back and forth at least once to increase the flow resistance. In this embodiment, the bypass section 2222 is U-shaped.

[0057] In this embodiment, if Figure 2 As shown, the single cell capture structure 221 is a single cell capture chamber, and the opening of the single cell capture chamber is connected to the tube wall of the inflow section 2221.

[0058] In other optional embodiments, such as Figure 3 As shown, the single-cell capture structure 221 is a single-cell capture channel. This single-cell capture channel is generally linear, with its inlet connected to the wall of the inflow section 2221, and its outlet connected to the wall of the outflow section 2223. The inlet of the single-cell capture channel is larger than its outlet, and the outlet is smaller than the passage size of a single cell. This prevents a single cell 3 from entering the channel and being discharged through its outlet.

[0059] like Figure 2 As shown, the inlet of the single-cell outlet channel 223 is connected to the inflow section 2221 of the bypass channel, and the outlet is connected to the cell culture chamber 23. The sorted single cells 3 are introduced into the cell culture chamber 23 through the single-cell outlet channel 223. In this embodiment, the passage size of the single-cell outlet channel 223 is set to allow only one single cell to pass through.

[0060] like Figure 1 As shown, the cell culture chamber 23 is connected to the cell sorting structure 22 through the single cell outlet channel 223, and is also connected to the second nozzle 25. The sorted single cells 3 enter the cell culture chamber 23 through the single cell outlet channel 223, and are cultured into cell groups therein, and the cell groups are then exported by the second nozzle 25. As an example, the height of the cell culture chamber 23 is set to accommodate only a single layer of cells to ensure the arrangement of single-layer cells during the cell culture process, which is helpful for subsequent cell counting and fluorescence analysis. As an example, a micro-column array 4 is provided between the cell culture chamber 23 and the second sample inlet 12 to prevent the loss of cells during the culture process. The micro-column array 4 includes a plurality of micro-columns arranged at intervals, and the material of the micro-columns can be silicon or silicon dioxide.

[0061] As an example, the first nozzle 24 and the second nozzle 25 can be selected from a group consisting of a thermal bubble nozzle, a piezoelectric nozzle, a PDMS (polydimethylsiloxane) microvalve, a solenoid valve, and a peristaltic pump, with a thermal bubble nozzle being preferred. A heating film is integrated at the bottom of the channel, and the instantaneous high temperature of the heating film is used to vaporize the liquid above the thermal bubble nozzle, thereby generating bubbles that drive the liquid flow and eject it from the thermal bubble nozzle. Subsequently, the subsequent liquid is replenished under the action of capillary force, thereby providing power for the continuous flow of the liquid. Each functional unit 2 is provided with a separate first nozzle 24 and second nozzle 25, so that the liquid flow of each functional unit 2 can be individually controlled, and the overall flow resistance will not increase significantly with the increase in the number of functional units, thereby making the number of functional units unlimited or less limited, achieving a higher throughput. In addition, the use of a thermal bubble nozzle as a single cell extraction component not only enables the rapid and high-throughput extraction of single cells, but also causes less damage to the cells, enabling the gentle extraction of single cells.

[0062] As an example, the microfluidic chip provided in this embodiment includes a flow channel layer, wherein a first sample inlet 11, a second sample inlet 12, a cell flow channel 21, a cell sorting structure 22, and a cell culture chamber 23 are all located in the flow channel layer. In this embodiment, the material of the flow channel layer can be selected from one of a silicon dioxide film, a non-photosensitive dry film, and a photosensitive dry film. Preferably, the flow channel layer is made of a silicon dioxide film or a non-photosensitive dry film material to avoid the influence of traditional dry film autofluorescence on cell sorting. Among them, the non-photosensitive dry film can be made of spin-on glass (SOG, a polysilane spin-coated material), non-photosensitive epoxy resin, non-photosensitive polyimide, and the like.

[0063] As an example, the microfluidic chip provided in this embodiment has a second direction ( Figure 1 A plurality of first injection ports 11, second injection ports 12 and functional areas can be provided in the Y-axis direction as shown, and adjacent first injection ports 11 and second injection ports 12 are separated from each other. Such a setting can achieve high throughput of the microfluidic chip.

[0064] The working principle of the microfluidic chip provided in this embodiment is as follows:

[0065] Combine Figure 1 and Figure 2 As shown, during the cell sorting process, a cell suspension is added to the first sample inlet 11. Driven by the first nozzle 24, the cell suspension flows through the first cell flow channel 211 and captures single cells in the cell sorting structure 22. Specifically, since the flow resistance of the single cell capture structure 221 is smaller than the flow resistance of the bypass section 2222, the cells flowing through the single cell capture structure 221 preferentially flow to the single cell capture structure 221, thereby capturing single cells 3. The excess cell suspension flows through the second cell flow channel 212 and is finally discharged by the first nozzle 24. It should be noted that Figure 1 The single cell capture structure 221 is described as a single cell capture chamber. When the single cell capture structure 221 is a single cell capture channel, refer to Figure 3 shown.

[0066] Next, cell culture is performed. Cell culture fluid is added to the second inlet 12. Nutrients in the cell culture fluid diffuse into the cell culture chamber 23. The second nozzle 25 is activated to release the captured single cells 3 and introduce them into the cell culture chamber 23. After culturing in the cell culture chamber 23 for a period of time, the single cells 3 become a cell population.

[0067] Finally, cell population screening is performed. Immunomagnetic beads and fluorescent antibodies are introduced into the cell culture chamber 23. Combined with fluorescence microscopy, the titer of cell secreted proteins is characterized. Cell populations with high titers are selected from the chip and exported to the well plate through the second nozzle 25.

[0068] Example 2

[0069] This embodiment provides a packaging structure, such as Figures 4a-4b As shown, it includes a base 100 , a base 200 , a chip 300 and a circuit board 400 .

[0070] As an example, the base 100 has a first surface 110 and a second surface 120 opposite to each other, and is provided with a first liquid inlet 101 and a second liquid inlet 102 penetrating the first surface 110 and the second surface 120. In this embodiment, the base 100 can be made of ceramic, stainless steel, plastic, or other materials.

[0071] As an example, the base 200 is located on the first surface 110 of the base, and the base 200 is bonded to the base 100 by thermosetting glue; Figure 5 As shown, the base 200 has a first injection slot 201 and a second injection slot 202, wherein the first injection slot 201 is provided corresponding to the first liquid inlet 101, and the second injection slot 202 is provided corresponding to the second liquid inlet 102. The first injection slot 201 is used to introduce a cell suspension into the chip 300. After flowing through the first injection slot 201 and the first liquid inlet 101, the cell suspension enters the chip 300. The second injection slot 202 is used to introduce a cell culture fluid into the chip 300. After flowing through the second injection slot 202 and the second liquid inlet 102, the cell culture fluid enters the chip 300.

[0072] As an example, chip 300 is located on the second surface 120 of the base, and chip 300 is the microfluidic chip provided in Example 1. Its specific structure can be referred to the description of Example 1 and will not be repeated here. As an example, the first sample inlet 11 in chip 300 is provided corresponding to the first liquid inlet 101, and the second sample inlet 12 in chip 300 is provided corresponding to the second liquid inlet 102.

[0073] In this embodiment, the second surface 120 of the base is further provided with a glue dispensing groove and a glue overflow prevention dam (not shown) for bonding the chip 300. Specifically, an appropriate amount of thermosetting glue is evenly dispensed into the glue dispensing groove, and the chip 300 and the second surface 120 of the base are aligned and bonded. After bonding, a heat curing process is performed to fully cure the glue and prevent the chip 300 from shifting during the subsequent packaging process.

[0074] As an example, circuit board 400 is electrically connected to chip 300 and serves to connect chip 300 to an external control system. In this embodiment, circuit board 400 is a flexible circuit board. Double-sided tape or glue can be used to bond the flexible circuit board to base 100, to which chip 300 is attached. Wire bonding or thermal compression bonding of pads are then used to electrically connect the chip 300 to the flexible circuit board. The flexible circuit board is then connected to an external control system to control chip operation. After wire bonding or thermal compression bonding of pads, the wires are protected with UV thermosetting glue, and UV pre-curing and thermal curing are performed.

[0075] As an example, in the packaging structure provided in this embodiment, all packaging materials involved are biocompatible and biosterilizable.

[0076] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the present invention. Anyone skilled in the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by one of ordinary skill in the art without departing from the spirit and technical principles disclosed herein are intended to be covered by the claims of the present invention.

Claims

1. A microfluidic chip, characterized in that: The device comprises a first injection port, a second injection port and a functional area, wherein the first injection port and the second injection port both extend along a first direction, and the functional area is arranged between the first injection port and the second injection port; The functional area includes a plurality of functional units arranged along a first direction, wherein the functional units include a cell flow channel, a cell sorting structure, a cell culture chamber, a first nozzle, and a second nozzle, wherein the cell flow channel, the cell sorting structure, and the cell culture chamber are sequentially arranged along a second direction perpendicular to the first direction, and the cell flow channel is connected to the first injection port, the cell culture chamber is connected to the second injection port, and a microcolumn array is provided between the cell culture chamber and the second injection port; The cell flow channel comprises a first cell flow channel and a second cell flow channel arranged in parallel in a first direction, wherein one end of the first cell flow channel is connected to the first injection port, and the other end is connected to the inlet of the cell sorting structure; one end of the second cell flow channel is connected to the outlet of the cell sorting structure, and the other end is connected to the first nozzle; The cell culture chamber is connected to the cell sorting structure and the second nozzle. The cell sorting structure sorts single cells from the cell suspension. The single cells enter the cell culture chamber and are cultured into cell clusters. The cell clusters are then discharged from the second nozzle. The cell sorting structure is provided with a single cell capture structure, a bypass channel and a single cell export channel. The single cell capture structure is used to capture a single cell in the cell suspension. The single cell enters the cell culture chamber through the single cell export channel, and the remaining cell suspension enters the second cell flow channel through the bypass channel.

2. The microfluidic chip according to claim 1, characterized in that The detour channel includes an inflow section, a detour section and an outflow section that are sequentially connected, and the flow directions of the inflow section and the outflow section are opposite.

3. The microfluidic chip according to claim 2, characterized in that: The single cell capture structure is a single cell capture channel, the inlet of the single cell capture channel is connected to the tube wall of the inflow section, and the outlet of the single cell capture channel is connected to the tube wall of the outflow section.

4. The microfluidic chip according to claim 3, characterized in that The inlet size of the single cell capture channel is larger than the outlet size of the single cell capture channel, and the outlet size of the single cell capture channel is set to be smaller than the passing size of the single cell.

5. The microfluidic chip according to claim 2, characterized in that: The single cell capturing structure is a single cell capturing chamber, and the opening of the single cell capturing chamber is connected to the tube wall of the inflow section.

6. The microfluidic chip according to claim 1, characterized in that The first nozzle and the second nozzle are selected from one of a thermal bubble nozzle, a piezoelectric nozzle, a PDMS (polydimethylsiloxane) microvalve, a solenoid valve and a peristaltic pump.

7. The microfluidic chip according to claim 1, characterized in that The microfluidic chip includes a flow channel layer, and the first sample inlet, the second sample inlet, the cell flow channel, the cell sorting structure, and the cell culture chamber are all located in the flow channel layer. The material of the flow channel layer is selected from one of silicon dioxide film, non-photosensitive dry film, and photosensitive dry film.

8. The microfluidic chip according to claim 1, characterized in that The height of the cell culture chamber is set to be greater than or equal to the cell size.

9. The microfluidic chip according to claim 1, characterized in that: The microfluidic chip is provided with a plurality of the first injection ports, the second injection ports and the functional areas in the second direction, and adjacent first injection ports and second injection ports are separated from each other.

10. A packaging structure, characterized in that: include: A base having a first surface and a second surface opposite to each other, and a first liquid inlet and a second liquid inlet penetrating the first surface and the second surface; A base, located on the first surface of the base, having a first sample injection slot and a second sample injection slot, wherein the first sample injection slot is correspondingly arranged with the first liquid inlet, and the second sample injection slot is correspondingly arranged with the second liquid inlet; A chip located on the second surface of the base, wherein the chip is the microfluidic chip according to any one of claims 1 to 9, wherein the first sample inlet in the microfluidic chip is correspondingly arranged with the first liquid inlet, and the second sample inlet in the microfluidic chip is correspondingly arranged with the second liquid inlet; A circuit board is electrically connected to the chip.

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