Infant plant lip balm with soothing effect and preparation method thereof

Abstract

The invention provides a soothing effect of infant plant lipstick and a preparation method thereof, wherein the components include, by mass percentage, 20-30% of olive oil, 20-30% of camellia oil, 10-20% of rapeseed oil, 10-20% of coconut oil, 10-15% of sunflower wax, 4-6% of beeswax, 3-5% of modified attapulgite, 2-6% of phytosterol oleate, 1-2% of plant squalane, 0.2%-1% of tocopherol, and 0.5-1% of white pondweed seed oil. The preparation method is as follows: sunflower wax and beeswax are taken, stirred at a speed of 30-50rpm, heated to 80-85°C to make them clear and transparent, modified attapulgite is added, ultrasonic stirring and high-speed grinding are performed, the remaining other components are added, 70-75°C is maintained, the stirring speed is 30-50rpm, after the material body is completely transparent, the material is filtered out and canned, and the finished product is obtained by cooling at -5-0°C for 3-5min. The ingredients of the present invention are all of natural origin, which are milder and safer than mineral oils and mineral waxes, are harmless to the body, have a soothing effect, prevent itching, burning, epidermal peeling, pain and the like on the lips, and are suitable for use by infants and young children.

Description

Technical Field

[0001] The invention relates to the field of daily cosmetics, and in particular to a soothing plant lipstick for infants and young children and a preparation method thereof. Background Art

[0002] The lips have no hair follicles, sebaceous glands, etc. and are the only non-stratum corneum mucous membrane in the body that is exposed to the outside for a long time. Their ability to resist changes in the external environment is poor. Therefore, our lips are easily prone to peeling, or even cracking and bleeding due to dryness, coldness and other climates.

[0003] Lip care is receiving more and more attention from people. Lip balm is a must-have cosmetic in people's daily life. After use, it can make lips soft and shiny. There are many kinds of lip balms on the market, but mineral wax and synthetic oils are generally used as the base materials of lip balms, such as vaseline, mineral oil, and ceresin. Artificial pigments and fragrances are also added. These ingredients cannot nourish the lip skin, and also affect the metabolism of the lip skin due to poor air permeability. Long-term use is not good for the body. Patent CN110755302A provides a moisturizing lip balm prepared from water, glycerin, polyethylene, synthetic wax, sunflower seed wax, hydrogenated polyisobutylene, pigment, pearl powder, silica, ethylhexylglycerin, caprylyl glycol, polydimethylsiloxane, and PEG-10 polydimethylsiloxane, which contains mineral wax and mineral oil.

[0004] For infants and young children, their skin is more sensitive and fragile, and their demand for lip care is more frequent. Therefore, there is an urgent need for a baby lip care product that does not contain ingredients such as mineral wax, mineral oil and synthetic oil. The main function of this product is to provide lasting moisturization to the lip skin, while soothing the lip skin and preventing adverse phenomena such as dryness, peeling and cracking of the lip skin. Summary of the invention

[0005] 1. Technical issues to be resolved

[0006] In view of the deficiencies in the prior art, the present invention aims to provide a soothing plant lip balm for infants and young children and a preparation method thereof, thereby solving the problem that the existing infant and young children lip balm contains raw materials such as mineral wax and mineral oil.

[0007] (II) Technical solution

[0008] To achieve the above objectives, the present invention proposes a solution:

[0009] The invention discloses a soothing plant lipstick for infants and young children, which comprises the following components by mass percentage: 20-30% olive oil, 20-30% camellia seed oil, 10-20% rapeseed oil, 10-20% coconut oil, 10-15% sunflower wax, 4-6% beeswax, 3-5% modified attapulgite, 2-6% phytosterol oleate, 1-2% plant squalane, 0.2-1% tocopherol and 0.5-1% meadowfoam seed oil.

[0010] A method for preparing a soothing botanical lip balm for infants and young children, comprising the following steps:

[0011] S1: Take the sunflower wax and beeswax in the composition, start stirring, heat to 80-85°C to make it clear and transparent.

[0012] Solid particles;

[0013] S2: Add modified attapulgite, stir ultrasonically, and after preliminary mixing, place the paste in a ball mill and grind at high speed for 10 minutes at a grinding speed of 2500 rpm;

[0014] S3: After the mixture is completely dissolved, olive oil, camellia oil, rapeseed oil, coconut oil, phytosteryl oleate, plant squalane, tocopherol, and meadowfoam seed oil are added, and the temperature is maintained at 70-75°C and stirred until the mixture is completely dissolved.

[0015] The material body is completely transparent;

[0016] S4: Filter the material, then can and cool it to obtain the finished product.

[0017] Furthermore, the stirring speed of step S1 is 30-50 rpm, the ultrasonic stirring power of step S2 is 60 kHz and the stirring speed is 400 rpm, and the stirring speed of step S3 is 30-50 rpm.

[0018] Furthermore, the cooling temperature of step S4 is -5 to 0° C., and the cooling time is 3 to 5 minutes.

[0019] Further, the preparation method of the modified attapulgite is as follows:

[0020] S11: Take attapulgite powder with a purity higher than 99.9%, and then take 1% to 5% of the mass of attapulgite oil.

[0021] The rod stone and grease are mixed and ground in a high-speed ball mill for 3 to 10 minutes;

[0022] S12: After the grinding is completed, the oil / attapulgite mixture is washed with ether for 1 to 2 times and with n-hexane for 3 to 4 times in sequence to obtain modified attapulgite;

[0023] S13: The product is filtered and washed with ultrapure water for 3 to 5 times, and spray-dried to obtain modified attapulgite with a particle size of not less than 3000 meshes.

[0024] Modified attapulgite: Using a special proportion of oil molecules to graft the surface of attapulgite is conducive to the fusion of attapulgite and natural oils, increasing its loading capacity for active ingredients, thereby improving the uniformity of active ingredients in the product and the sustained release effect on the skin. The good uniformity and sustained release effect of modified attapulgite can make the moisturizing and soothing ingredients in oils and other ingredients play a gentle and lasting role on the skin.

[0025] Rapeseed oil: rich in α-linolenic acid, it not only delays oil oxidation, but also has important functional activities such as antibacterial, anti-tumor, anti-inflammatory, and antiviral. Inflammation is a physiological response of the body to stimulation or injury, usually accompanied by redness, swelling, heat, pain, and dysfunction of local tissues. The anti-inflammatory effect can usually be understood as having a soothing effect. Rapeseed oil has an anti-inflammatory effect and a regulatory effect on inflammatory reactions, which can reduce the degree and duration of inflammation. The addition of rapeseed oil in the present invention not only has a good moisturizing effect on the skin, but also has a good soothing effect. Olive oil: rich in squalene and essential fatty acids with excellent affinity to the skin, it is quickly absorbed and effectively maintains skin elasticity and moisture; the rich monounsaturated fatty acids and vitamins E, K, A, D, etc. and phenolic antioxidants contained in olive oil can eliminate facial wrinkles, prevent skin aging, and have the effects of skin care, hair care, and prevention and treatment of chapped hands and feet.

[0026] Camellia seed oil: Nearly 70%-80% of its components are the same as human skin, and it has a very good affinity with the skin, so it is absorbed by the skin. When used in skin care products, it can make the skin softer and more elastic, and has a good moisturizing and skin care effect. Camellia seed oil can effectively absorb ultraviolet rays and is a natural sunscreen skin care product.

[0027] Sunflower Seed Oil: Contains a variety of vitamins and minerals, as well as oleic acid, such as Omega-9 fatty acids. Omega-9 fatty acids are an essential acid that prevents moisture loss in the lips and has a highly moisturizing effect.

[0028] Coconut oil: coagulant, natural coconut flavor. Coconut oil has good coagulability and has its own coconut fragrance. Coconut oil can be used to replace coagulants and flavors, which not only reduces costs but also makes the product more gentle.

[0029] Sunflower wax: Pure natural plant wax, obtained from dewaxing sunflower seed oil. It is composed of long-chain saturated esters, has a high melting point, and can improve the hardness and crystal structure of the formula. Sunflower wax has excellent oil binding ability, and has a light, non-sticky feel on the skin, and has good film-forming and emollient properties.

[0030] Beeswax: Contains water, lipids, free fatty alcohols, free fatty acids, carbohydrates, carotenoids, vitamin A and aromatic substances. It is a natural and harmless substance that can lock in skin moisture and moisturize the skin. It also has natural antibacterial properties and will not deteriorate at high temperatures.

[0031] Plant squalane: Antioxidant and thermal stability. Squalane is the lipid closest to human sebum, which can form a natural barrier on the skin surface and inhibit the peroxidation of skin lipids.

[0032] Tocopherol: A natural antioxidant with repairing and moisturizing effects.

[0033] (III) Beneficial effects

[0034] The present invention provides a soothing plant lipstick for infants and young children and a preparation method thereof. The components are all of pure natural plant origin, without mineral oils or synthetic oils, and are mild and non-irritating, without harm to the lips of the body, and have a soothing effect, preventing the occurrence of lip cracking, drying, peeling and the like. The invention is a relatively mild and safe lip care product for infants and young children. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 This is the ELISA standard curve for TNF-α content. DETAILED DESCRIPTION

[0036] The present invention is further described below in conjunction with the examples, but the present invention is not limited thereby. The experimental methods in the following examples are conventional methods unless otherwise specified.

[0037] Preparation of modified attapulgite:

[0038] Sources

[0039] Attapulgite powder: purity higher than 99.9%, fineness 3000 mesh.

[0040] Organic solvents: anhydrous ether, n-hexane, analytical grade, commercially available.

[0041] To prepare the modified attapulgite, follow the steps below:

[0042] S11: Take attapulgite powder with a purity higher than 99.9%, and then take 1-5% of the mass of attapulgite oil.

[0043] The stone and grease are mixed and ground in a high-speed ball mill for 3 to 10 minutes;

[0044] S12: After the grinding is completed, the oil / attapulgite mixture is washed with ether for 1 to 2 times and with n-hexane for 3 to 4 times in sequence to obtain modified attapulgite;

[0045] S13: The product is filtered and washed with ultrapure water for 3 to 5 times, and spray-dried to obtain modified attapulgite with a particle size of not less than 3000 meshes.

[0046] Table 1 Exemplary products of modified attapulgite

[0047]

[0048] Embodiment 1:

[0049] A soothing botanical lip balm for infants and young children, the masses of the components of which are shown in Table 2.

[0050] Embodiment 2:

[0051] A soothing botanical lip balm for infants and young children, the masses of the components of which are shown in Table 2.

[0052] Embodiment 3:

[0053] A soothing botanical lip balm for infants and young children, the masses of the components of which are shown in Table 2.

[0054] Embodiment 4:

[0055] A soothing botanical lip balm for infants and young children, the masses of the components of which are shown in Table 2.

[0056] Comparative Example 1:

[0057] A soothing botanical lip balm for infants and young children, the masses of the components of which are shown in Table 2.

[0058] Comparative Example 2:

[0059] A soothing botanical lip balm for infants and young children, the masses of the components of which are shown in Table 2.

[0060] Table 2 Mass of each component in the examples and comparative examples

[0061] Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 olive oil 24kg 24kg 24kg 24kg 24kg 24kg Coconut Oil 10kg 10kg 10kg 10kg 10kg 10kg Sunflower Seed Wax 15kg 15kg 15kg 15kg 15kg 15kg Camellia Seed Oil 20kg 20kg 20kg 20kg 20kg 20kg Canola Oil 10kg 10kg 10kg 10kg 10kg 10kg Phytosteryl Oleate 5kg 5kg 5kg 5kg 5kg 5kg Lignum Meadowfoam Seed Oil 1kg 1kg 1kg 1kg 1kg 1kg beeswax 5kg 5kg 5kg 5kg 5kg 5kg Plant Squalane 5kg 5kg 5kg 5kg 5kg 5kg Tocopherol 5kg 5kg 5kg 5kg 5kg 5kg Rapeseed oil modified attapulgite 4kg 0 0 0 0 0 Camellia oil modified attapulgite 0 4kg 0 0 0 0 Olive oil modified attapulgite 0 0 4kg 0 0 0 Coconut oil modified attapulgite 0 0 0 4kg 0 0 Attapulgite 0 0 0 0 4kg 0

[0062] The method for preparing a soothing botanical lip balm for infants and young children in the above-mentioned Examples 1 to 4 and Comparative Examples 1 to 2 specifically comprises the following steps:

[0063] S1: Take 15 kg of sunflower seed wax and 5 kg of beeswax, add them into a reaction container, then start heating and stirring, stirring at a speed of 30-50 rpm, and heat to 80-85°C to make the material completely dissolved and clear;

[0064] S2: Add modified attapulgite and stir with ultrasonic power of 60kHz and stirring speed of 400rpm. After preliminary mixing, place the paste in a ball mill and grind at high speed for 10min at a grinding speed of 2500rpm.

[0065] S3: After it is completely dissolved, add 24kg olive oil, 20kg camellia oil, 10kg canola oil, 10kg coconut oil, 5kg phytosteryl oleate, 5kg plant squalane, 5kg vitamin E, and 1kg white meadowfoam seed oil.

[0066] Then maintain the temperature at 70-75°C and stir at a speed of 30-50 rpm until the material becomes clear and transparent;

[0067] S4: After all physical and chemical indicators are qualified, filter and discharge the material and then can it, and cool it at -5-0℃ for 3-5min to obtain the product.

[0068] The experimental design is described as follows:

[0069] Comparison Groups 1 and 2, in order to verify the effect of modified attapulgite: except for the attapulgite component as a variable factor, the quality of the components in Comparison Groups 1-2 and Example 1 is consistent.

[0070] Relief detection method:

[0071] Detection basis: The skin will produce dryness, erythema and other phenomena under external stimulation. The TNF-α content is detected by LPS (1μg / mL) stimulation and measured by ELISA detection method.

[0072] Experimental instruments and materials: mouse macrophage RAW264.7, high-glucose DMEM culture medium (10% fetal bovine serum), TNF-α inflammatory factor ELISA kit, MW160CO2 incubator, SW-CJ-1FD clean bench, TS100 inverted microscope, SpectraMAX i3 microplate reader.

[0073] Sample treatment: Weigh 2.00 g of sample and add 2 mL of high-glucose DMEM culture medium, extract at 37°C overnight. The extract is regarded as 100% extract, and then diluted to 50% extract and 25% extract.

[0074] Positive control: dexamethasone (batch number 923H0511, manufacturer Solarbio), solvent dimethyl sulfoxide, concentration 100 g / ml.

[0075] Negative control stimulus: LPS lipopolysaccharide (lot number: D16C04, manufacturer absin, solvent phosphate buffered saline solution, concentration 1 μg / mL).

[0076] Positive and negative group configuration:

[0077] Positive control group: 10 mg of dexamethasone was dissolved in 1 mL of dimethyl sulfoxide solution to obtain the dexamethasone stock solution with a concentration of 10 mg / mL; the dexamethasone stock solution was diluted to 100 μg / mL at a ratio of 1:100 using high-glucose DMEM culture medium containing LPS as the positive control working solution.

[0078] Negative control group (stimulant): 10 mg LPS was dissolved in 2 mL DMSO solution to obtain LPS mother solution with a concentration of 5 mg / mL; the LPS mother solution was diluted to 1 μg / mL with high-glucose DMEM culture medium at a ratio of 1:5000.

[0079] Test method (cytotoxicity test):

[0080] Cell seeding: 1×10 4 The cells were seeded into a 96-well plate at a seeding density of 100 cells / well and incubated overnight in an incubator (37° C., 5% CO 2 ).

[0081] Experimental grouping: The experiment set up a zero adjustment group, a solvent control group, a positive control group, and a sample group. In the sample group, 8 concentration gradients were set for the samples, and 3 replicate wells were set under each concentration gradient.

[0082] Solution preparation: Prepare sample working solutions of different concentrations according to the test concentration settings (100% extract, 50% extract, 25% extract, 12.5% ​​extract, 6.25% extract, 3.13% extract, 1.56% extract, 0.78% extract).

[0083] Sampling: Sampling is performed when the cell plating rate in the 96-well plate reaches 40%-60%. The zeroing group is 200 μL high-glucose DMEM culture fluid (10% fetal bovine serum) per well; the solvent control group is inoculated with cells in 200 μL high-glucose DMEM culture fluid (10% fetal bovine serum) per well, the positive control group is inoculated with cells in 200 μL high-glucose DMEM culture fluid (10% fetal bovine serum) containing 10% DMSO per well, and the sample group is inoculated with cells in 200 μL high-glucose DMEM culture fluid (10% fetal bovine serum) containing samples of corresponding concentrations per well. After sampling, the 96-well plate is placed in an incubator (37°C, 5% CO2) for incubation.

[0084] Detection: After incubating the cells for 24 hours, discard the supernatant, add MTT working solution (0.5 mg / mL), incubate at 37°C in the dark for 4 hours, discard the supernatant, add 150 μL DMSO to each well, and read the OD value at 490 nm.

[0085] Calculation of relative cell viability:

[0086] Relative cell viability (%) = [(sample well OD - withering well OD) / (solvent control well OD - withering well OD)] * 100%

[0087] Soothing effect test:

[0088] TNF-α is a representative inflammatory factor. By detecting the inhibitory effect of a soothing plant lip balm on the production of TNF-α, the inhibitory effect of the lip balm on inflammation can be tested, that is, the soothing effect of the lip balm can be tested.

[0089] Table 3 Experimental groups

[0090]

[0091] Cell inoculation: Collect cells in logarithmic growth phase and set the cell density at 1×10 4 Each well was inoculated into a 96-well plate and cultured in a 37°C, 5% CO2 incubator for 24 h.

[0092] Sampling: Sampling is performed when the cell plating rate in the 96-well plate reaches 40%-60%. The blank control group is cell culture medium, 200 μL per well, the negative control group is cell culture medium containing LPS, 200 μL per well, the positive control group is culture medium containing positive control and LPS, 200 μL per well, and the sample group is culture medium containing a certain concentration of the test substance and LPS, 200 μL per well. After sampling, the 96-well plate is placed in an incubator (37°C, 5% CO2) for 24 hours.

[0093] ELISA detection: After the culture, the cell culture medium was collected in a 1.5 mL Eppendorf tube, centrifuged at 4°C and 1000×g, and the supernatant was used for TNF-α detection.

[0094] Experimental test results and analysis:

[0095] Table 4 Cytotoxicity results

[0096]

[0097]

[0098] The trend of cell survival rate decreasing with the increase of compound concentration is not obvious and remains at a high level. This shows that the toxicity of the product compound is very small and it is very safe.

[0099] TNF-α content detection:

[0100] Table 5 TNF-α content detection raw data

[0101]

[0102]

[0103] Standard curve: Draw a standard curve with the concentration of the standard as the ordinate and the OD value as the abscissa, such as Figure 1 As shown. According to the OD of the sample 450 The corresponding concentration was found and the TNF-α content of the sample was calculated. Finally, the average value of the three replicate wells in each group was taken as the final TNF-α content result.

[0104] Results and judgments:

[0105] Inhibition rate (%) = (1-T / C) × 100%

[0106] T—average value of TNF-α content of the tested substance;

[0107] C—Average value of TNF-α content in negative control.

[0108] Table 6 TNF-α detection results

[0109]

[0110] As shown in Table 6, when the concentration of the test sample was 25% to 100% of the extract, the TNF-α content of each group decreased compared with the negative control, which had the effect of inhibiting the TNF-α content, that is, it had a soothing effect. From the analysis of the experimental results, the surface loading capacity of the modified attapulgite was greatly improved compared with the unmodified attapulgite, and the improvement of the soothing effect of the product was more obvious. Among the modified attapulgite, the modification effect of rapeseed oil was the best.

[0111] In summary, the present invention provides a soothing effect of infant plant lipstick and its preparation method and application, the composition of which includes by mass percentage: olive oil 20-30%, camellia oil 20-30%, rapeseed oil 10-20%, coconut oil 10-20%, sunflower wax 10-15%, beeswax 4-6%, modified attapulgite 3-5%, phytosterol oleate 2-6%, plant squalane 1-2%, tocopherol 0.2-1%, white pondweed seed oil 0.5-1%. All the ingredients of the present invention are from natural plants, do not contain chemical synthetic ingredients such as mineral oils and mineral waxes, the composition is more gentle and safe, and has no harm to the lips of the body, and also takes into account the soothing effect, can prevent itching, burning, epidermal exfoliation, pain and other phenomena of the lips, and is suitable for infants with sensitive skin.

[0112] It should be understood that the application of the present invention is not limited to the above examples. For ordinary technicians in this field, improvements or changes can be made based on the above description. All these improvements and changes should fall within the scope of protection of the claims attached to the present invention.

Claims

1. A soothing botanical lip balm for infants and young children, characterized in that: The composition is as follows in terms of mass percentage: 20-30% olive oil, 20-30% camellia seed oil, 10-20% rapeseed oil, 10-20% coconut oil, 10-15% sunflower wax, 4-6% beeswax, 3-5% modified attapulgite, 2-6% phytosterol oleate, 1-2% plant squalane, 0.2-1% tocopherol, and 0.5-1% white meadowfoam seed oil; the preparation method of the plant lipstick for infants and young children with soothing effect comprises the following steps: S1: taking the composition Sunflower wax and beeswax, stir, heat to 80-85°C, make them clear and transparent without solid particles; S2: add modified attapulgite, stir ultrasonically, after preliminary mixing, place the paste in a ball mill and grind at high speed for 10 minutes, the grinding speed is 2500rpm; S3: after it is completely dissolved, add olive oil, camellia oil, canola oil, coconut oil, phytosterol oleate, plant squalane, tocopherol, white meadowfoam seed oil, keep the temperature at 70-75°C, and stir, until the material is completely transparent; S4: filter the material, then can and cool it to obtain the finished product; the preparation method of the modified attapulgite comprises the following steps: S11: take attapulgite powder with a purity higher than 99.9%, and then take 1% to 5% of the mass of attapulgite oil, mix the attapulgite and the oil in a high-speed ball mill and grind for 3 to 10 minutes; S12: after the grinding, wash the oil / attapulgite mixture with ether for 1 to 2 times and with n-hexane for 3 to 4 times in sequence to obtain modified attapulgite; S13: The product is filtered, washed with ultrapure water for 3 to 5 times, and spray-dried to obtain modified attapulgite with a particle size of not less than 3000 mesh; the oil in step S11 and step S12 is rapeseed oil, the stirring speed in step S1 is 30-50rpm, the ultrasonic stirring in step S2 has an ultrasonic power of 60kHz and a stirring speed of 400rpm, the stirring speed in step S3 is 30-50rpm, the cooling temperature in step S4 is -5 to 0°C, and the cooling time is 3 to 5min.

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