A method for interplanting colorful leaves under agarwood forest
By planting colorful leaves under the agarwood forest and using organic base fertilizer, water fertilizer and biopesticides, the problems of weed breeding, pests and soil erosion in the agarwood forest are solved, and efficient land utilization and healthy growth of agarwood trees are achieved.
Patent Information
- Application Number
- CN202410478574.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-19
- Publication Date
- 2025-02-18
- Estimated Expiration
- 2044-04-19
AI Technical Summary
After afforestation, the gaps in the forest are prone to breed weeds and pests, and are prone to soil erosion and affect the growth of agarwood trees.
Plant colorful leaves under the agarwood forest, and select suitable forest land and seedlings, apply organic base fertilizer, spray water and fertilizer, and spray bioinsecticides to prevent and control diseases and land management.
Effectively utilize land resources, improve land utilization, and increase returns; the growth of colorful leaves provides nutrients and promotes the growth of agarwood trees; reduces water evaporation and keeps the soil moist; reduces disease rate, avoids direct sunlight, and promotes the healthy growth of colorful leaves.
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Abstract
Description
Technical Field
[0001] The invention relates to the technical field of agriculture and animal husbandry cultivation, and in particular to a method for interplanting variegated leaves under an agarwood forest. Background Art
[0002] Agarwood, also known as white wood fragrance, honey fragrance, and sinking water fragrance, belongs to the Thymelaeaceae family. Domestic agarwood is white wood fragrance, also known as daughter fragrance, tooth fragrance, six hemp tree, and agarwood. It is a precious medicinal plant unique to my country and a national second-level protected plant. It is known as the treasure of Chinese traditional medicine. It has a wide range of uses and is the main medicine for many traditional Chinese medicine formulas. It is pungent, slightly warm, non-toxic, and has the effects of lowering qi and phlegm, moistening the spleen, nourishing the kidneys and clearing the liver. Because agarwood is a woody plant, it has a long growth cycle and slow forest formation. In addition, the investment in agarwood afforestation is large, and there is basically no income before the agarwood is formed. At the same time, after early afforestation, the area of open space between forests is large, which is easy to breed weeds and pests and diseases, and it is easy to cause soil erosion.
[0003] Pandan leaf, also known as pandan leaf, is a tropical plant with a unique aroma and colorful leaves, hence the name. This plant is popular in Southeast Asia and is often used in cooking and pastry making, adding a fresh fragrance and bright green to food. Pandan leaf is not only fragrant, but also rich in nutrients and has certain medicinal value. It can clear away heat and moisten the lungs, increase appetite, help digestion, nourish the stomach, enhance human resistance, and improve the body's metabolic capacity. In addition, pandan leaves are also rich in nutrients such as linoleic acid, squalene, vitamin K3, phytol, and artemisia argyi. With people's pursuit of healthy diet and natural ingredients, the application of pandan leaves in the food industry has become more and more extensive, becoming an emerging healthy ingredient and condiment. Whether in desserts, drinks or staple foods, pandan leaves can bring unique flavor and visual enjoyment to food. However, if pandan leaves are planted alone, direct strong sunlight will damage the leaves, causing sunburn or yellowing. Summary of the invention
[0004] In view of this, the present invention proposes a method for interplanting variegated leaves under an agarwood forest to solve the above-mentioned problem.
[0005] The technical solution of the present invention is achieved as follows: a method for interplanting variegated leaves under an agarwood forest, comprising the following steps:
[0006] S1. Forest selection: Select agarwood forests with an altitude of 200-400m, a forest density of 0.4-0.5, and a tree age of 4-8 years as intercropping forests;
[0007] S2. Selection of variegated leaf seedlings: Select high-quality and healthy tillers or variegated leaf seedlings cultivated by tissue culture;
[0008] S3. Forest land preparation: remove weeds, stones and branches under the agarwood forest, till the soil, dig planting trenches and apply organic base fertilizer;
[0009] S4. Planting of colorful leaf seedlings: Plant the colorful leaf seedlings into the planting trench, backfill the soil and compact it, keep the colorful leaf seedlings upright, and water them to establish roots;
[0010] S5. Forest management: Fertilization management, spraying water and fertilizer once every 80-90 days, the water and fertilizer application rate is 120-150Kg / mu;
[0011] S6. Pest and disease control: spray biological pesticides 30-40 days after planting, and then spray biological pesticides every 80-90 days, the spraying amount of the biological pesticide is 10-12 kg / mu;
[0012] S7. Harvesting: The first harvest is 90-100 days after planting, harvesting the fresh leaves of the Bancai leaves below the 6th leaf from the top of the Bancai seedlings, and harvesting once every 40-60 days;
[0013] Furthermore, in step S1, the row spacing of the agarwood forest is 2.0-2.5 m, and the plant spacing is 2.0-2.5 m.
[0014] Furthermore, in step S2, the tillering seedlings are 15-20 cm tall and the tissue culture seedlings are 20-25 cm tall.
[0015] Furthermore, the organic base fertilizer in step S3 includes the following components in parts by weight: 30-50 parts of chicken manure, 10-20 parts of bone meal, 10-20 parts of plant ash, 5-15 parts of perlite powder, and 0.2-0.6 parts of indolebutyric acid.
[0016] Furthermore, in step S4, the planting time of the colorful leaf seedlings is a certain day between early March and late April, the planting row spacing is 40-50 cm, and the plant spacing is 40-50 cm.
[0017] Further, the water fertilizer in step S5 includes the following components in parts by weight: 90-100 parts of water, 4-6 parts of ammonium bicarbonate, 3-5 parts of superphosphate, 3-5 parts of potassium chloride, 0.5-1.0 parts of tyrosine, 0.5-1.0 parts of methionine, and 0.04-0.08 parts of methyl jasmonate.
[0018] Furthermore, the forest management in step S5 also includes irrigation management, and the irrigation management includes watering the variegated leaves once a day within 3 days of planting, and then watering once every 12-15 days.
[0019] Furthermore, the pest control in step S6 is to control powdery mildew, anthracnose, aphids, and mites.
[0020] Furthermore, in step S6, the biological pesticide is composed of rotenone, osthole and azadirachtin in a mass ratio of (1-3):(1.0-1.5):(0.5-1.0).
[0021] Furthermore, the harvesting time in step S7 is 7:00-10:00 in the morning or 16:00-18:30 in the afternoon.
[0022] Compared with the prior art, the invention has the following beneficial effects: the invention interplants the colorful leaves under the agarwood forest to effectively utilize land resources, improve land utilization rate, and increase income. During the growth of the colorful leaves, the organic matter and trace elements produced can provide nutrients for the agarwood tree and promote its growth. At the same time, the coverage of the colorful leaves can also reduce the evaporation of water, keep the soil moist, and be beneficial to the growth of the agarwood tree. And after planting the colorful leaves, the breeding of weeds is reduced, and the spraying of biological pesticides can not only inhibit the diseases and insect pests of the colorful leaves, but also reduce the disease rate of the agarwood forest. Planting the colorful leaves under the agarwood forest also avoids direct sunlight on the colorful leaves, which is beneficial to its healthy growth. At the same time, the air humidity under the agarwood forest is high, and the humid air can reduce the evaporation of water from the leaves of the colorful leaves, so the leaves of the colorful leaves can maintain moisture and glossiness. In addition, the agarwood forest can also serve as a natural barrier to reduce the impact of natural disasters such as typhoons, heavy rains, and frost on the colorful leaves. The present invention applies an organic base fertilizer composed of chicken manure, bone meal, plant ash, perlite powder, and indolebutyric acid when planting the pandan leaf seedlings, which not only provides rich nutrition for the growth of pandan leaf seedlings and agarwood, but also promotes the rapid rooting of pandan leaf seedlings, improves its survival rate, and promotes the robust growth of pandan leaves. The water fertilizer applied by the present invention, wherein tyrosine and methionine are absorbed by pandan leaves as auxiliary factors in the synthesis process of 2-acetyl-1-pyrroline, a characteristic aroma of pandan leaves, and methyl jasmonate is absorbed by pandan leaves as a signal molecule to increase the expression of 2-acetyl-1-pyrroline synthesis-related genes, promote the synthesis of 2-acetyl-1-pyrroline in pandan leaves, and make the characteristic aroma of pandan leaves more intense. The biopesticide applied by the present invention not only reduces the problem of soil pollution and residue, but also can reduce the emergence of the problem of insect resistance. DETAILED DESCRIPTION
[0023] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.
[0024] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.
[0025] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources. Example 1
[0026] A method for interplanting variegated leaves under an agarwood forest comprises the following steps:
[0027] S1. Forest selection: Select a 4-year-old agarwood forest with an altitude of 200m, a forest density of 0.4, a row spacing of 2.0m, a plant spacing of 2.0m, and a tree age of 4 years as the intercropping forest;
[0028] S2. Selection of colorful leaf seedlings: Select high-quality and healthy tillering colorful leaf seedlings, with a seedling height of 15 cm;
[0029] S3. Forest land preparation: remove weeds, stones and branches under the agarwood forest, till the soil, dig planting trenches and apply organic base fertilizer, which includes the following components by weight: 30 parts of chicken manure, 10 parts of bone meal, 10 parts of plant ash, 5 parts of perlite powder and 0.2 parts of indolebutyric acid;
[0030] S4. Planting of colorful leaf seedlings: On March 5, plant the colorful leaf seedlings into the planting ditch, backfill the soil and compact it, keep the colorful leaf seedlings upright, water them to establish roots, and plant them with a row spacing of 40 cm and a plant spacing of 40 cm;
[0031] S5. Forest management: Fertilization management is carried out, water and fertilizer are sprayed once every 80 days, the water and fertilizer application rate is 120 kg / mu, and the water and fertilizer include the following weight-part array components: 90 parts of water, 4 parts of ammonium bicarbonate, 3 parts of superphosphate, 3 parts of potassium chloride, 0.5 parts of tyrosine, 0.5 parts of methionine, and 0.04 parts of methyl jasmonate; forest management also includes irrigation management, and the irrigation management is to water the colorful leaf seedlings once a day within 3 days after planting, and then water them once every 12 days.
[0032] S6. Pest and disease control: Pest and disease control is to prevent and control powdery mildew, anthracnose, aphids and mites. Spray biological pesticides 300 days after planting, and then spray once every 80 days. The spraying amount of the biological pesticide is 10 kg / mu. The biological pesticide is composed of rotenone, osthole and azadirachtin in a mass ratio of 1.0:1.0:0.5.
[0033] S7. Harvesting: The first harvest is on the 90th day after planting, and the fresh leaves of the Bancai leaves below the 6th leaf on the top of the Bancai seedlings are harvested. The next harvest is once every 40 days; the harvesting time is 7:00-10:00 in the morning. Example 2
[0034] A method for interplanting variegated leaves under an agarwood forest comprises the following steps:
[0035] S1. Forest selection: Select a 5-year-old agarwood forest with an altitude of 400m, a forest density of 0.5, a row spacing of 2.5m, a plant spacing of 2.5m, and a tree age of 5 years as the intercropping forest;
[0036] S2. Selection of colorful leaf seedlings: Select high-quality and healthy tillering colorful leaf seedlings, with a seedling height of 20cm;
[0037] S3. Forest land preparation: remove weeds, stones and branches under the agarwood forest, till the soil, dig planting trenches and apply organic base fertilizer, which includes the following components by weight: 50 parts of chicken manure, 20 parts of bone meal, 20 parts of plant ash, 15 parts of perlite powder and 0.6 parts of indolebutyric acid;
[0038] S4. Planting of colorful leaf seedlings: On March 15, plant the colorful leaf seedlings into the planting ditch, backfill the soil and compact it, keep the colorful leaf seedlings upright, water them to establish roots, and plant them with a row spacing of 50 cm and a plant spacing of 50 cm;
[0039] S5. Forest management: Fertilization management is carried out, water and fertilizer are sprayed once every 90 days, the water and fertilizer application rate is 150 kg / mu, and the water and fertilizer include the following weight-part array components: 100 parts of water, 6 parts of ammonium bicarbonate, 5 parts of superphosphate, 5 parts of potassium chloride, 1.0 part of tyrosine, 1.0 part of methionine, and 0.08 part of methyl jasmonate; forest management also includes irrigation management, and the irrigation management is to water the colorful leaf seedlings once a day within 3 days after planting, and then water them once every 15 days.
[0040] S6. Pest and disease control: Pest and disease control is to prevent and control powdery mildew, anthracnose, aphids and mites. Spray biological pesticides 40 days after planting, and then spray once every 90 days. The spraying amount of the biological pesticide is 12 kg / mu. The biological pesticide is composed of rotenone, osthole and azadirachtin in a mass ratio of 3.0:1.5:1.0.
[0041] S7. Harvesting: The first harvest is on the 100th day after planting, when fresh leaves below the 6th leaf from the top of the Bancai seedlings are harvested, and then harvested once every 60 days; the harvesting time is 7:00-10:00 in the morning. Example 3
[0042] A method for interplanting variegated leaves under an agarwood forest comprises the following steps:
[0043] S1. Forest selection: Select a 6-year-old agarwood forest with an altitude of 300m, a forest density of 0.5, a row spacing of 2.3m, a plant spacing of 2.3m, and a tree age of 6 years as the intercropping forest;
[0044] S2. Selection of colorful leaf seedlings: Select high-quality and healthy tillering colorful leaf seedlings, with a seedling height of 18cm;
[0045] S3. Forest land preparation: remove weeds, stones and branches under the agarwood forest, till the soil, dig planting trenches and apply organic base fertilizer, which includes the following components by weight: 40 parts of chicken manure, 15 parts of bone meal, 15 parts of plant ash, 10 parts of perlite powder and 0.4 parts of indolebutyric acid;
[0046] S4. Planting of colorful leaf seedlings: On March 25, plant the colorful leaf seedlings into the planting ditch, backfill the soil and compact it, keep the colorful leaf seedlings upright, water them to establish roots, and plant them at a row spacing of 45 cm and a plant spacing of 45 cm;
[0047] S5. Forest management: Fertilization management is carried out, water and fertilizer are sprayed once every 90 days, the water and fertilizer application rate is 135 kg / mu, and the water and fertilizer include the following components in groups by weight: 95 parts of water, 5 parts of ammonium bicarbonate, 4 parts of superphosphate, 4 parts of potassium chloride, 0.8 parts of tyrosine, 0.8 parts of methionine, and 0.06 parts of methyl jasmonate; forest management also includes irrigation management, and the irrigation management is to water the colorful leaf seedlings once a day within 3 days after planting, and then water them once every 14 days.
[0048] S6. Pest and disease control: Pest and disease control is to prevent and control powdery mildew, anthracnose, aphids and mites. Spray biological pesticides 40 days after planting, and then spray once every 90 days. The spraying amount of the biological pesticide is 11 kg / mu. The biological pesticide is composed of rotenone, osthole and azadirachtin in a mass ratio of 2.0:1.3:0.8.
[0049] S7. Harvesting: The first harvest is on the 100th day after planting, when fresh leaves below the 6th leaf from the top of the Bancai seedlings are harvested, and then harvested once every 60 days; the harvesting time is 7:00-10:00 in the morning. Example 4
[0050] A method for interplanting variegated leaves under an agarwood forest comprises the following steps:
[0051] S1. Forest selection: Select a 5-year-old agarwood forest with an altitude of 250m, a forest density of 0.4, a row spacing of 2.5m, a plant spacing of 2.0m, and a tree age of 5 years as the intercropping forest;
[0052] S2. Selection of variegated leaf seedlings: Select high-quality and healthy variegated leaf seedlings cultivated by tissue culture, with a seedling height of 20 cm;
[0053] S3. Forest land preparation: remove weeds, stones and branches under the agarwood forest, till the soil, dig planting trenches and apply organic base fertilizer, which includes the following components by weight: 35 parts of chicken manure, 12 parts of bone meal, 12 parts of plant ash, 8 parts of perlite powder and 0.3 parts of indolebutyric acid;
[0054] S4. Planting of colorful leaf seedlings: On April 10, plant the colorful leaf seedlings into the planting ditch, backfill the soil and compact it, keep the colorful leaf seedlings upright, water them to establish roots, and plant them with a row spacing of 50 cm and a plant spacing of 40 cm;
[0055] S5. Forest management: Fertilization management is carried out, and water and fertilizer are sprayed once every 80-90 days. The water and fertilizer application rate is 130 kg / mu. The water and fertilizer include the following components in different weights: 92 parts of water, 4 parts of ammonium bicarbonate, 3.5 parts of superphosphate, 3.5 parts of potassium chloride, 0.7 parts of tyrosine, 0.7 parts of methionine, and 0.05 parts of methyl jasmonate. Forest management also includes irrigation management, and the irrigation management is to water the colorful leaf seedlings once a day within 3 days after planting, and then water them once every 13 days.
[0056] S6. Pest and disease control: Pest and disease control is to prevent and control powdery mildew, anthracnose, aphids and mites. Spray biological pesticides 30-40 days after planting, and then spray biological pesticides once every 83 days. The spraying amount of the biological pesticide is 10.5 kg / mu. The biological pesticide is composed of rotenone, osthole and azadirachtin in a mass ratio of 1.5:1.1:0.6.
[0057] S7. Harvesting: The first harvest is on the 93rd day after planting, when fresh leaves below the 6th leaf from the top of the Bancai seedlings are harvested, and then harvested once every 45 days; the harvesting time is 16:00-18:30 in the afternoon. Example 5
[0058] A method for interplanting variegated leaves under an agarwood forest comprises the following steps:
[0059] S1. Forest selection: Select agarwood forests with an altitude of 350m, a forest density of 0.5, a row spacing of 2.0m, a plant spacing of 2.5m, and an age of 8 years as intercropping forests;
[0060] S2. Selection of variegated leaf seedlings: Select high-quality and healthy variegated leaf seedlings or those cultivated by tissue culture, with a seedling height of 25 cm;
[0061] S3. Forest land preparation: remove weeds, stones and branches under the agarwood forest, till the soil, dig planting trenches and apply organic base fertilizer, which includes the following components by weight: 45 parts of chicken manure, 18 parts of bone meal, 18 parts of plant ash, 13 parts of perlite powder and 0.5 parts of indolebutyric acid;
[0062] S4. Planting of colorful leaf seedlings: On April 25, plant the colorful leaf seedlings into the planting ditch, backfill the soil and compact it, keep the colorful leaf seedlings upright, water them to establish roots, and plant them at a row spacing of 40 cm and plant spacing of 50 cm;
[0063] S5. Forest management: Fertilization management is carried out, water and fertilizer are sprayed once every 88 days, the water and fertilizer application rate is 140 kg / mu, and the water and fertilizer include the following components in groups by weight: 98 parts of water, 5 parts of ammonium bicarbonate, 4.5 parts of superphosphate, 4.5 parts of potassium chloride, 0.9 parts of tyrosine, 0.9 parts of methionine, and 0.07 parts of methyl jasmonate; forest management also includes irrigation management, and the irrigation management is to water the colorful leaf seedlings once a day within 3 days after planting, and then water them once every 15 days.
[0064] S6. Pest and disease control: Pest and disease control is to prevent and control powdery mildew, anthracnose, aphids and mites. Spray biological pesticides 30-40 days after planting, and then spray biological pesticides once every 88 days. The spraying amount of the biological pesticide is 11.5 kg / mu. The biological pesticide is composed of rotenone, osthole and azadirachtin in a mass ratio of 2.5:1.3:0.9.
[0065] S7. Harvesting: The first harvest is on the 98th day after planting, and the fresh leaves below the 6th leaf on the top of the Bancai seedlings are harvested. The next harvest is once every 55 days; the harvesting time is 16:00-18:30 in the afternoon. Comparative Example 1
[0066] The difference between this comparative example and Example 3 is that in step S6, an equal amount of chlordimeform is sprayed instead of the biological insecticide.
[0067] Comparative Example 2
[0068] The difference between this comparative example and Example 3 is that the biological insecticide is composed of rotenone and osthole in a mass ratio of 1:1. Comparative Example 3
[0069] The difference between this comparative example and Example 3 is that the biological insecticide is composed of rotenone and azadirachtin in a mass ratio of 1.0:0.5. Comparative Example 4
[0070] The difference between this comparative example and Example 3 is that the biological insecticide is composed of osthole and azadirachtin in a mass ratio of 1.0:0.5.
[0071] The yields of the colorful leaves of Examples 1-5 and Comparative Examples 1-4 before the first five times were counted, and the specific yield values are shown in Table 1.
[0072] Table 1
[0073]
[0074] As can be seen from Table 1, the method for interplanting Pandanus variegata under the agarwood forest of the present invention can harvest a higher yield of Pandanus variegata, especially the yield in Example 3 is better.
[0075] By comparing Example 3 with Comparative Example 1, although more colorful leaves were harvested in Comparative Example 1 at the first harvest, as time went by, the resistance of pathogenic bacteria and pests gradually increased, the damage of chlordimeform to pathogenic bacteria and pests gradually weakened, and the production of colorful leaves gradually decreased. The biological pesticide used in the present invention, rotenone, affects the respiratory metabolism of aphids and mites, inhibits the electron transport chain in the cells of aphids and mites, and then reduces the level of adenosine triphosphate in the organism, so that aphids and mites cannot get energy supply, and their actions are delayed, paralyzed and die. Osthole can inhibit spore germination and formation, directly destroy the formed mycelium and sporangium, rupture the mycelium and lose the ability to infect, and effectively prevent and treat anthrax and powdery mildew. In addition, neem alcohol acts on the nervous system of aphids and mites, interferes with their normal nerve conduction, causes aphids and mites to show symptoms such as paralysis and slow movement, kills aphids and mites, reduces the density of aphids and mites, and thus reduces the harm to the colorful leaves. Rotenone, osthole, and neem alcohol in the biological pesticide of the present invention work together through different mechanisms, and have significant effects in preventing and controlling powdery mildew, anthracnose, aphids, and mites, thereby promoting a higher yield of colorful leaves.
[0076] By comparing Example 3 with Comparative Examples 2-4, the total output of Comparative Example 2 before 5 times decreased by 6.5% compared with that of Example 3, the total output of Comparative Example 3 before 5 times decreased by 8.9% compared with that of Example 3, and the total output of Comparative Example 4 before 5 times decreased by 6.8% compared with that of Example 3. It can be seen that each of rotenone, osthole and azadirachtin plays a very important role in the biological pesticide. The lack of one of them will weaken the control effect. Only the joint action of the three can play the best value in controlling powdery mildew, anthracnose, aphids and mites.
[0077] In order to verify the effect of the present invention on enhancing the characteristic aroma of Paniculata, comparative examples 5 and 6 were set up for comparison with embodiment 3. Comparative Example 5
[0078] This comparative example is different from implementation 3 in that the water-fertilizer component does not contain tyrosine, methionine, or methyl jasmonate. Comparative Example 6
[0079] The difference between this comparative example and Example 3 is that the water fertilizer comprises the following components in parts by weight: 120 parts of water, 10 parts of ammonium bicarbonate, 10 parts of superphosphate, 8 parts of potassium chloride, 0.3 parts of tyrosine, 0.3 parts of methionine, and 0.01 parts of methyl jasmonate.
[0080] The 2-acetyl-1-pyrroline content of the first harvested pandan leaves of Implementation 3 and Comparative Examples 5-6 was measured 5 times each, and the average value of the 2-acetyl-1-pyrroline content of the 5 times was calculated. The results are shown in Table 2.
[0081] Table 2
[0082]
[0083] As can be seen from Table 2, the 2-acetyl-1-pyrroline content in the colorful leaves harvested by the method of the present invention is the highest.
[0084] Compared with Comparative Example 5, the 2-acetyl-1-pyrroline content of Example 3 increased by 77.3%. This is because, in Example 3, water-fertilizer containing tyrosine, methionine and methyl jasmonate was applied, wherein tyrosine is a precursor substance for the synthesis of 2-acetyl-1-pyrroline. In the variegated leaves, tyrosine is converted into 2-acetyl-1-pyrroline through a series of enzymatic reactions. When the tyrosine content in the variegated leaves increases, these enzymatic reactions can be carried out more efficiently, thereby synthesizing more 2-acetyl-1-pyrroline. Methionine can affect the expression and activity of related enzymes in the variegated leaves, thereby further regulating the synthesis process of 2-acetyl-1-pyrroline. Methyl jasmonate can activate the defense response in the variegated leaves, including improving the activity of antioxidant enzymes, inducing the synthesis of secondary metabolites, etc. In this process, methyl jasmonate also promotes the synthesis of 2-acetyl-1-pyrroline. In addition, methyl jasmonate can further enhance the role of tyrosine and methionine in the synthesis of 2-acetyl-1-pyrroline by regulating their metabolic pathways. Therefore, through the combined action of tyrosine, methionine, and methyl jasmonate, the content of 2-acetyl-1-pyrroline in the leaves of Paniculata variegata was significantly increased.
[0085] Compared with Comparative Example 6, Example 3 shows that the 2-acetyl-1-pyrroline content of Comparative Example 6 is reduced by 43.6%. It can be seen that only under a specific ratio, the synergistic effect between the components in the water fertilizer can play the best effect of increasing the content of 2-acetyl-1-pyrroline, otherwise the effect of increasing the content of 2-acetyl-1-pyrroline will be greatly reduced.
[0086] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims
1. A method for interplanting colorful leaves under an agarwood forest, characterized in that: The following steps are involved: S1. Forest selection: Select agarwood forests with an altitude of 200-400m, a forest density of 0.4-0.5, and a tree age of 4-8 years as intercropping forests; S2. Selection of variegated leaf seedlings: Select high-quality and healthy tillers or variegated leaf seedlings cultivated by tissue culture; S3. Forest land preparation: remove weeds, stones, branches under the agarwood forest, plow the soil, dig planting trenches and apply organic base fertilizer; the organic base fertilizer includes the following weight groups of components: 30-50 parts of chicken manure, 10-20 parts of bone meal, 10-20 parts of wood ash, 5-15 parts of perlite powder, 0.2-0.6 parts of indolebutyric acid; S4. Planting of colorful leaf seedlings: Plant the colorful leaf seedlings into the planting trench, backfill the soil and compact it, keep the colorful leaf seedlings upright, and water them to establish roots; S5. Forest management: Fertilization management, spraying water and fertilizer every 80-90d, the water and fertilizer application rate is 120-150Kg / mu; the water and fertilizer include the following weight groups of components: 90-100 parts of water, 4-6 parts of ammonium bicarbonate, 3-5 parts of superphosphate, 3-5 parts of potassium chloride, 0.5-1.0 parts of tyrosine, 0.5-1.0 parts of methionine, 0.04-0.08 parts of methyl jasmonate; S6. Pest and disease control: spray biological pesticides 30-40 days after planting, and then spray biological pesticides every 80-90 days, the spraying amount of the biological pesticide is 10-12 kg / mu; S7. Harvesting: The first harvest is 90-100 days after planting, harvesting the fresh leaves of the Bancai leaves below the 6th leaf from the top of the Bancai seedlings, and harvesting once every 40-60 days; In step S6, the biological pesticide is composed of rotenone, osthole and azadirachtin in a mass ratio of (1-3):(1.0-1.5):(0.5-1.0).
2. The method for interplanting variegated leaves under an agarwood forest as claimed in claim 1, characterized in that: In step S1, the row spacing of the agarwood forest is 2.0-2.5 m, and the plant spacing is 2.0-2.5 m.
3. The method for interplanting variegated leaves under an agarwood forest as claimed in claim 1, characterized in that: In step S2, the tillering seedlings have a height of 15-20 cm, and the tissue culture seedlings have a height of 20-25 cm.
4. The method for interplanting variegated leaves under an agarwood forest as claimed in claim 1, characterized in that: In the step S4, the planting time of the colorful leaf seedlings is a certain day between early March and late April, the planting row spacing is 40-50 cm, and the plant spacing is 40-50 cm.
5. The method for interplanting variegated leaves under an agarwood forest as claimed in claim 1, characterized in that: The forest management in step S5 also includes irrigation management, and the irrigation management is to water the variegated leaves once a day within 3 days of planting, and then water them once every 12-15 days.
6. The method for interplanting variegated leaves under an agarwood forest as claimed in claim 1, characterized in that: The pest control in step S6 is to control powdery mildew, anthracnose, aphids and mites.
7. The method for interplanting variegated leaves under an agarwood forest as claimed in claim 1, characterized in that: The harvesting time in step S7 is 7:00-10:00 in the morning or 16:00-18:30 in the afternoon.