Biosynthesis method for de novo synthesis of glabridin and strain

Abstract

The invention relates to the field of biological engineering and technology, in particular to an enzyme for synthesizing glabridin, a biological synthesis method of glabridin and a bacterial strain. The invention discloses a series of glabridin synthesizing enzymes (phenylalanine ammonialyase, cinnamic acid-4-hydrogenase, tyrosine ammonialyase, p-coumaric acid coenzyme A ligase, chalcone synthase, chalcone reductase, chalcone isomerase, isoflavone synthase, 2-hydroxyisoflavone dehydratase, 4 '-oxymethyltransferase, 4'-hydroxymethyltransferase and 4 '-hydroxymethyltransferase) derived from glycyrrhiza glabra for the first time. ) can be used in the preparation of a compound (e.g., isoflavone 2 '-hydrogenase, isoflavone reductase, Vissisoketoreductase, isoprenyltransferase, pterocarpin reductase, oxidative cyclase, demethylase, cytochrome P450 reductase, and the like). The de novo synthesis of the glabridin in a microbial cell factory is realized for the first time, and the application prospect of the glabridin biological manufacturing industry is greatly promoted.

Description

Technical Field

[0001] The present invention relates to the field of bioengineering and technology, and in particular to an enzyme for synthesizing glabridin, a biosynthetic method and a strain for synthesizing glabridin from scratch. Background Art

[0002] Glabridin is a flavonoid compound extracted from licorice. It possesses a variety of physiological activities, including anti-inflammatory, antioxidant, antibacterial, and tyrosinase inhibition. Consequently, glabridin has broad applications in skin whitening and skincare, and is often incorporated into various cosmetics. Glabridin also exhibits some anticancer activity and has potential applications in anti-allergy, antiviral, and cardiovascular protection. Due to its natural origin and multiple pharmacological properties, glabridin has become a research hotspot in drug development, medical aesthetics, and functional foods.

[0003] Currently, the extraction of glabridin primarily relies on isolation and purification from the roots of Glycyrrhiza glabra L. However, the glabridin content is low, accounting for approximately 0.09% of the root mass. The extraction process requires multiple steps, including drying, grinding, and pulverization, and the use of ultrasound and microwave-assisted extraction methods. This results in a complex process, low extraction efficiency, high costs, and severe environmental pollution. Furthermore, licorice has a well-developed root system that acts as a windbreak and sand fixation agent. It is primarily distributed in Central Asia and northwestern China, and in western desert and semi-desert regions such as Gansu, Xinjiang, and Inner Mongolia in my country, where it has important ecological applications. However, the root-digging extraction method severely disrupts the distribution of licorice vegetation and the local ecological environment. Currently, licorice used primarily comes from imported sources and cultivated plants. However, cultivated licorice has disadvantages such as a long growth cycle, occupies a large area of arable land, and is susceptible to climatic conditions, severely limiting the production of glabridin. Current methods for obtaining glabridin are unsustainable and conflict with the goal of building a green and environmentally friendly ecosystem. New methods are needed to address these challenges by transforming the raw material source.

[0004] Therefore, an efficient and environmentally friendly method for synthesizing glycyrrhizin is urgently needed in the relevant technology. Summary of the Invention

[0005] The present invention solves at least one of the problems of the related art from the following aspects.

[0006] The first aspect of the present application provides a method for the biosynthesis of glycyrrhizin, comprising: providing a biological host; causing the biological host to express one or more enzymes selected from the group consisting of: phenylalanine ammonia lyase, cinnamate-4-hydrogenase, tyrosine ammonia lyase, p-coumarate Coenzyme A ligase, chalcone synthase, chalcone reductase, chalcone isomerase, isoflavone synthase, 2-hydroxyisoflavone dehydratase, 4'-oxymethyltransferase, isoflavone 2'-hydrogenase, isoflavone reductase, visnezyl ketone reductase, isoprenyl transferase, pterostilbene reductase, oxidative cyclase, demethylase, and cytochrome P450 reductase to obtain an engineered biological host; and culturing the engineered biological host to obtain glycyrrhizin.

[0007] In some embodiments, the phenylalanine ammonia lyase is derived from one or more of alfalfa and Glycyrrhiza glabra, wherein the phenylalanine ammonia lyase derived from alfalfa is MtPAL, whose accession number in the NCBI database is XP_003590471.1, and the phenylalanine ammonia lyase derived from Glycyrrhiza glabra is GgPAL, whose amino acid sequence is shown in SEQ ID NO: 1.

[0008] In some embodiments, the cinnamate 4-hydrogenase is derived from one or more of Arabidopsis thaliana and Glycyrrhiza glabra, wherein the cinnamate 4-hydrogenase derived from Arabidopsis thaliana is AtC4H, whose accession number in the NCBI database is NP_180607.1, and the cinnamate 4-hydrogenase derived from Glycyrrhiza glabra is GgC4H, whose amino acid sequence is shown in SEQ ID NO: 2.

[0009] In some embodiments, the tyrosine ammonia lyase is SeTAL from Saccharothrix hispanica, whose accession number in the NCBI database is ABC88669.1.

[0010] In some embodiments, the p-coumarate-CoA ligase is derived from one or more of parsley and Glycyrrhiza glabra, wherein the p-coumarate-CoA ligase derived from parsley is Pc4CL, whose accession number in the NCBI database is CAA31697.1, and the p-coumarate-CoA ligase derived from Glycyrrhiza glabra is Gg4CL, whose amino acid sequence is shown in SEQ ID NO: 3.

[0011] In some embodiments, the chalcone synthase is derived from one or more of petunia and Glycyrrhiza glabra, wherein the chalcone synthase derived from petunia is PhCHS, whose accession number in the NCBI database is CAA32731.1, and the chalcone synthase derived from Glycyrrhiza glabra is GgCHS, whose amino acid sequence is shown in SEQ ID NO:4.

[0012] In some embodiments, the chalcone reductase is GgCHR from Glycyrrhiza glabra, whose amino acid sequence is shown in SEQ ID NO:5.

[0013] In some embodiments, the chalcone isomerase is derived from one or more of alfalfa, Glycyrrhiza glabra, and Arabidopsis thaliana, wherein the chalcone isomerase derived from alfalfa is MsCHI, whose accession number in the NCBI database is AAB41524.1, the chalcone isomerase derived from Glycyrrhiza glabra is GgCHI, whose amino acid sequence is shown in SEQ ID NO: 6, and the chalcone isomerase derived from Arabidopsis thaliana is AtCHIL, whose accession number in the NCBI database is NP_850770.1.

[0014] In some embodiments, the isoflavone synthase is GgIFS from Glycyrrhiza glabra, whose amino acid sequence is shown in SEQ ID NO:7.

[0015] In some embodiments, the 2-hydroxyisoflavone dehydratase is GgHID from Glycyrrhiza glabra, whose amino acid sequence is shown in SEQ ID NO:8.

[0016] In some embodiments, the 4'-O-methyltransferase is derived from one or more of Pueraria lobata and Glycyrrhiza glabra, wherein the 4'-O-methyltransferase derived from Pueraria lobata is P1OMT9, whose accession number in the NCBI database is AKW47171.1, and the 4'-O-methyltransferase derived from Glycyrrhiza glabra is GgOMT, whose amino acid sequence is shown in SEQ ID NO: 9.

[0017] In some embodiments, the isoflavone 2'-hydrogenase is derived from one or more of soybean and Glycyrrhiza glabra, wherein the isoflavone 2'-hydrogenase derived from soybean is GeCYP81E1, whose UniProtKB / Swiss-Prot number is P93147.2, and the isoflavone 2'-hydrogenase derived from Glycyrrhiza glabra is GgI2'H, whose amino acid sequence is shown in SEQ ID NO:10.

[0018] In some embodiments, the isoflavone reductase is derived from one or more of alfalfa and Glycyrrhiza glabra, wherein the isoflavone reductase derived from alfalfa is MsIFR, whose GenBank number is CAA41106.1, and the isoflavone reductase derived from Glycyrrhiza glabra is GgIFR, whose amino acid sequence is shown in SEQ ID NO:11.

[0019] In some embodiments, the visconazole reductase is derived from one or more of alfalfa and Glycyrrhiza glabra, wherein the visconazole reductase derived from alfalfa is MsVR, whose UniProtKB / Swiss-Prot number is Q40316.1, and the visconazole reductase derived from Glycyrrhiza glabra is GgVR, whose amino acid sequence is shown in SEQ ID NO:12.

[0020] In some embodiments, the isoprenyltransferase is derived from one or more of Psoralea corylifolia and Glycyrrhiza glabra, wherein the isoprenyltransferase derived from Psoralea corylifolia is tPcM4DT, whose amino acid sequence is shown in SEQ ID NO: 13, and the isoprenyltransferase derived from Glycyrrhiza glabra is one or more of GgPT1, GgPT2, GgPT3, GgPT4, GgPT5, GgPT6, and GgPT7, whose amino acid sequences are shown in SEQ ID NO: 14, 15, 16, 17, 18, 19, and 20, respectively.

[0021] In some embodiments, the pterostilbene reductase is one or more of GgPTR1, GgPTR2, GgPTR3, GgPTR4, GgPTR5, GgPTR6, GgPTR7, and GgPTR8 from Glycyrrhiza glabra, and their amino acid sequences are shown in SEQ ID NOs: 21, 22, 23, 24, 25, 26, 27, and 28, respectively.

[0022] In some embodiments, the oxidative cyclase is one or more of GgOC1, GgOC2, GgOC3, GgOC4, GgOC5, GgOC6, GgOC7, and GgOC8 from Glycyrrhiza glabra, and their amino acid sequences are shown in SEQ ID NOs: 29, 30, 31, 32, 33, 34, 35, and 36, respectively.

[0023] In some embodiments, the demethylase is derived from one or more of Fusarium solani, Glycyrrhiza glabra, Glycyrrhiza uralensis, and Glycyrrhiza inflata, wherein the demethylase derived from Fusarium solani is NhDMT, and its amino acid sequence is shown in SEQ ID NO: 37; the demethylase derived from Glycyrrhiza glabra is one or more of GgDMT1, GgDMT2, GgDMT3, GgDMT4, GgDMT5, GgDMT6, GgDMT7, and GgDMT8, and their amino acid sequences are shown in SEQ ID NO: 38, 39, 40, 41, 42, 43, 44, and 45, respectively; the demethylase derived from Glycyrrhiza uralensis is one or more of GuDMT1, GuDMT2, and GuDMT3, and their amino acid sequences are shown in SEQ ID NO: As shown in NO:46, 47, and 48, the demethylase derived from Glycyrrhiza inflata is one or more of GiDMT1, GiDMT2, and GiDMT3, and their amino acid sequences are shown in SEQ ID NO:49, 50, and 51, respectively.

[0024] In some embodiments, the cytochrome P450 reductase is GuCPR2 from Glycyrrhiza uralensis, whose amino acid sequence is shown in SEQ ID NO:52.

[0025] In some embodiments, at least one of the phenylalanine ammonia lyase, cinnamate 4-hydrogenase, tyrosine ammonia lyase, p-coumarate CoA ligase, chalcone synthase, chalcone reductase, chalcone isomerase, isoflavone synthase, 2-hydroxyisoflavone dehydratase, 4'-oxymethyltransferase, isoflavone 2'-hydrogenase, isoflavone reductase, visconol reductase, prenyltransferase, pterostilbene reductase, oxidative cyclase, demethylase, and cytochrome P450 reductase is derived from Glycyrrhiza glabra.

[0026] In some embodiments, the biological host is selected from the group consisting of Gram-positive bacteria, Gram-negative bacteria, actinomycetes, yeast, filamentous fungi, and tobacco.

[0027] In some embodiments, the Gram-positive bacteria include Bacillus subtilis and Corynebacterium glutamicum, the Gram-negative bacteria include Escherichia coli, the actinomycetes include Streptomyces coelicolor, the yeast include Saccharomyces cerevisiae, Yarrowia lipolytica, and Pichia pastoris, the filamentous fungi include Aspergillus niger, and the tobacco includes Nicotiana benthamiana.

[0028] In some embodiments, the host organism is yeast, and the engineered host organism heterologously expresses:

[0029] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase tPcM4DT from Psoralea corylifolia, pterostilbene reductase GgPTR1 from Glycyrrhiza glabra, oxidative cyclase GgOC1 from Glycyrrhiza glabra, demethylase NhDMT from Fusarium solani (corresponding to GLA1 in the examples of the present application); or

[0030] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT1 from Glycyrrhiza glabra, pterostilbene reductase GgPTR2 from Glycyrrhiza glabra, oxidative cyclase GgOC2 from Glycyrrhiza glabra, demethylase GgDMT1 from Glycyrrhiza glabra (corresponding to GLA2 in the examples of the present application); or

[0031] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT2 from Glycyrrhiza glabra, pterostilbene reductase GgPTR3 from Glycyrrhiza glabra, oxidative cyclase GgOC3 from Glycyrrhiza glabra, demethylase GgDMT2 from Glycyrrhiza glabra (corresponding to GLA3 in the examples of the present application); or

[0032] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, isoprenyltransferase GgPT3 from Glycyrrhiza glabra, pterostilbene reductase GgPTR4 from Glycyrrhiza glabra, oxidative cyclase GgOC4 from Glycyrrhiza glabra, demethylase GgDMT3 from Glycyrrhiza glabra (corresponding to GLA4 in the examples of the present application); or

[0033] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT4 from Glycyrrhiza glabra, pterostilbene reductase GgPTR5 from Glycyrrhiza glabra, oxidative cyclase GgOC5 from Glycyrrhiza glabra, demethylase GgDMT4 from Glycyrrhiza glabra (corresponding to GLA5 in the examples of the present application); or

[0034] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT5 from Glycyrrhiza glabra, pterostilbene reductase GgPTR6 from Glycyrrhiza glabra, oxidative cyclase GgOC6 from Glycyrrhiza glabra, demethylase GgDMT5 from Glycyrrhiza glabra (corresponding to GLA6 in the examples of the present application); or

[0035] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT6 from Glycyrrhiza glabra, pterostilbene reductase GgPTR7 from Glycyrrhiza glabra, oxidative cyclase GgOC7 from Glycyrrhiza glabra, demethylase GgDMT6 from Glycyrrhiza glabra (corresponding to GLA7 in the examples of the present application); or

[0036] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT7 from Glycyrrhiza glabra, pterostilbene reductase GgPTR8 from Glycyrrhiza glabra, oxidative cyclase GgOC8 from Glycyrrhiza glabra, demethylase GgDMT7 from Glycyrrhiza glabra (corresponding to GLA8 in the examples of the present application); or

[0037] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT1 from Glycyrrhiza glabra, pterostilbene reductase GgPTR2 from Glycyrrhiza glabra, oxidative cyclase GgOC2 from Glycyrrhiza glabra, demethylase GgDMT8 from Glycyrrhiza glabra (corresponding to GLA9 in the examples of the present application); or

[0038] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT2 from Glycyrrhiza glabra, pterostilbene reductase GgPTR3 from Glycyrrhiza glabra, oxidative cyclase GgOC3 from Glycyrrhiza glabra, demethylase GuDMT1 from Glycyrrhiza glabra (corresponding to GLA10 in the examples of the present application); or

[0039] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, isoprenyltransferase GgPT3 from Glycyrrhiza glabra, pterostilbene reductase GgPTR4 from Glycyrrhiza glabra, oxidative cyclase GgOC4 from Glycyrrhiza glabra, demethylase GuDMT2 from Glycyrrhiza glabra (corresponding to GLA11 in the examples of the present application); or

[0040] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT4 from Glycyrrhiza glabra, pterostilbene reductase GgPTR5 from Glycyrrhiza glabra, oxidative cyclase GgOC5 from Glycyrrhiza glabra, demethylase GuDMT3 from Glycyrrhiza glabra (corresponding to GLA12 in the examples of the present application); or

[0041] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT5 from Glycyrrhiza glabra, pterostilbene reductase GgPTR6 from Glycyrrhiza glabra, oxidative cyclase GgOC6 from Glycyrrhiza glabra, demethylase GiDMT1 from Glycyrrhiza glabra (corresponding to GLA13 in the examples of the present application); or

[0042] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT6 from Glycyrrhiza glabra, pterostilbene reductase GgPTR7 from Glycyrrhiza glabra, oxidative cyclase GgOC7 from Glycyrrhiza glabra, demethylase GiDMT2 from Glycyrrhiza glabra (corresponding to GLA14 in the examples of the present application); or

[0043] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SetAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, isoprenyltransferase GgPT7 from Glycyrrhiza glabra, pterostilbene reductase GgPTR8 from Glycyrrhiza glabra, oxidative cyclase GgOC8 from Glycyrrhiza glabra, demethylase GiDMT3 from Glycyrrhiza glabra (corresponding to GLA15 in the examples of the present application);

[0044] Wherein, the substrate for biosynthesis of glabridin is selected from the group consisting of glucose, galactose and xylose.

[0045] In some embodiments, the biological host comprises: GLA1, GLA2, GLA3, GLA4, GLA9, GLA10, GLA11.

[0046] In some embodiments, the method for biosynthesizing glabridin further comprises: causing the biological host to express: a mutated 4'-O-methyltransferase from kudzu vine comprising one or more of the following mutations: F24Y, F307L, F307I, F307V, M311L, Y160P, and Y160L, wherein the amino acid sequence of the 4'-O-methyltransferase from kudzu vine has an accession number of AKW47171.1 in the NCBI database.

[0047] In some embodiments, the method for biosynthesis of glabridin further comprises: causing the biological host to express: a mutated ARO4 from Saccharomyces cerevisiae comprising the following mutation: K229L; and / or a mutated ARO7 from Saccharomyces cerevisiae comprising the following mutation: G141S, wherein the accession number of ARO4 from Saccharomyces cerevisiae in the NCBI database is NP_009808.1, and the accession number of ARO7 from Saccharomyces cerevisiae in the NCBI database is NP_015385.1.

[0048] In some embodiments, the method for biosynthesis of glabridin further comprises: causing the biological host to heterologously express or overexpress: a synthase of dimethylallyl pyrophosphate, wherein the synthesis gene of dimethylallyl pyrophosphate is selected from the group consisting of: acetyl-CoA acetyltransferase, 3-hydroxy-3-methylglutaryl-CoA synthase, hydroxymethylglutaryl-CoA reductase, mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylase, and dimethylallyl diphosphate isomerase.

[0049] In some embodiments, the method for biosynthesizing glabridin further comprises: causing the biological host to heterologously express or overexpress an enzyme selected from the group consisting of: 6-phosphate glucose dehydrogenase, malate dehydrogenase, and NADH-dependent hydroxymethylglutaryl-CoA reductase, wherein the accession number of the NCBI database for the 6-phosphate glucose dehydrogenase is NP_014158.1, the accession number of the NCBI database for the malate dehydrogenase is NP_012896.1, and the accession number of the NADH-dependent hydroxymethylglutaryl-CoA reductase is WP_011241944.1.

[0050] In some embodiments, the method for biosynthesis of glabridin further comprises: heterologously expressing or overexpressing acetyl-CoA carboxylase in the biological host.

[0051] In some embodiments, the method for biosynthesis of glabridin further comprises: causing the biological host to express a demethylase using a galactose-inducible promoter or a xylose-inducible promoter.

[0052] In a second aspect, the present application provides an engineered biological host, wherein the engineered biological host expresses one or more enzymes selected from the group consisting of: phenylalanine ammonia lyase, cinnamate-4-hydrogenase, tyrosine ammonia lyase, p-coumarate Coenzyme A ligase, chalcone synthase, chalcone reductase, chalcone isomerase, isoflavone synthase, 2-hydroxyisoflavone dehydratase, 4'-oxymethyltransferase, isoflavone 2'-hydrogenase, isoflavone reductase, visnezyl reductase, isoprenyl transferase, pterostilbene reductase, oxidative cyclase, demethylase, cytochrome P450 reductase,

[0053] At least one of the phenylalanine ammonia lyase, cinnamate 4-hydrogenase, tyrosine ammonia lyase, p-coumarate Coenzyme A ligase, chalcone synthase, chalcone reductase, chalcone isomerase, isoflavone synthase, 2-hydroxyisoflavone dehydratase, 4'-oxymethyltransferase, isoflavone 2'-hydrogenase, isoflavone reductase, visconol reductase, prenyltransferase, pterostilbene reductase, oxidative cyclase, demethylase and cytochrome P450 reductase is derived from Glycyrrhiza glabra.

[0054] In some embodiments, the phenylalanine ammonia lyase is derived from one or more of alfalfa and Glycyrrhiza glabra, wherein the phenylalanine ammonia lyase derived from alfalfa is MtPAL, whose accession number in the NCBI database is XP_003590471.1, and the phenylalanine ammonia lyase derived from Glycyrrhiza glabra is GgPAL, whose amino acid sequence is shown in SEQ ID NO: 1.

[0055] In some embodiments, the cinnamate 4-hydrogenase is derived from one or more of Arabidopsis thaliana and Glycyrrhiza glabra, wherein the cinnamate 4-hydrogenase derived from Arabidopsis thaliana is AtC4H, whose accession number in the NCBI database is NP_180607.1, and the cinnamate 4-hydrogenase derived from Glycyrrhiza glabra is GgC4H, whose amino acid sequence is shown in SEQ ID NO: 2.

[0056] In some embodiments, the tyrosine ammonia lyase is SeTAL from Saccharothrix hispanica, whose accession number in the NCBI database is ABC88669.1.

[0057] In some embodiments, the p-coumarate-CoA ligase is derived from one or more of parsley and Glycyrrhiza glabra, wherein the p-coumarate-CoA ligase derived from parsley is Pc4CL, whose accession number in the NCBI database is CAA31697.1, and the p-coumarate-CoA ligase derived from Glycyrrhiza glabra is Gg4CL, whose amino acid sequence is shown in SEQ ID NO: 3.

[0058] In some embodiments, the chalcone synthase is derived from one or more of petunia and Glycyrrhiza glabra, wherein the chalcone synthase derived from petunia is PhCHS, whose accession number in the NCBI database is CAA32731.1, and the chalcone synthase derived from Glycyrrhiza glabra is GgCHS, whose amino acid sequence is shown in SEQ ID NO:4.

[0059] In some embodiments, the chalcone reductase is GgCHR from Glycyrrhiza glabra, whose amino acid sequence is shown in SEQ ID NO:5.

[0060] In some embodiments, the chalcone isomerase is derived from one or more of alfalfa, Glycyrrhiza glabra, and Arabidopsis thaliana, wherein the chalcone isomerase derived from alfalfa is MsCHI, whose accession number in the NCBI database is AAB41524.1, the chalcone isomerase derived from Glycyrrhiza glabra is GgCHI, whose amino acid sequence is shown in SEQ ID NO: 6, and the chalcone isomerase derived from Arabidopsis thaliana is AtCHIL, whose accession number in the NCBI database is NP_850770.1.

[0061] In some embodiments, the isoflavone synthase is GgIFS from Glycyrrhiza glabra, whose amino acid sequence is shown in SEQ ID NO:7.

[0062] In some embodiments, the 2-hydroxyisoflavone dehydratase is GgHID from Glycyrrhiza glabra, whose amino acid sequence is shown in SEQ ID NO:8.

[0063] In some embodiments, the 4'-O-methyltransferase is derived from one or more of Pueraria lobata and Glycyrrhiza glabra, wherein the 4'-O-methyltransferase derived from Pueraria lobata is P1OMT9, whose accession number in the NCBI database is AKW47171.1, and the 4'-O-methyltransferase derived from Glycyrrhiza glabra is GgOMT, whose amino acid sequence is shown in SEQ ID NO: 9.

[0064] In some embodiments, the isoflavone 2'-hydrogenase is derived from one or more of soybean and Glycyrrhiza glabra, wherein the isoflavone 2'-hydrogenase derived from soybean is GeCYP81E1, whose UniProtKB / Swiss-Prot number is P93147.2, and the isoflavone 2'-hydrogenase derived from Glycyrrhiza glabra is GgI2'H, whose amino acid sequence is shown in SEQ ID NO:10.

[0065] In some embodiments, the isoflavone reductase is derived from one or more of alfalfa and Glycyrrhiza glabra, wherein the isoflavone reductase derived from alfalfa is MsIFR, whose accession number in the NCBI database is CAA41106.1, and the isoflavone reductase derived from Glycyrrhiza glabra is GgIFR, whose amino acid sequence is shown in SEQ ID NO:11.

[0066] In some embodiments, the visconazole reductase is derived from one or more of alfalfa and Glycyrrhiza glabra, wherein the visconazole reductase derived from alfalfa is MsVR, whose UniProtKB / Swiss-Prot number is Q40316.1, and the visconazole reductase derived from Glycyrrhiza glabra is GgVR, whose amino acid sequence is shown in SEQ ID NO:12.

[0067] In some embodiments, the isoprenyltransferase is derived from one or more of Psoralea corylifolia and Glycyrrhiza glabra, wherein the isoprenyltransferase derived from Psoralea corylifolia is tPcM4DT, whose amino acid sequence is shown in SEQ ID NO: 13, and the isoprenyltransferase derived from Glycyrrhiza glabra is one or more of GgPT1, GgPT2, GgPT3, GgPT4, GgPT5, GgPT6, and GgPT7, whose amino acid sequences are shown in SEQ ID NO: 14, 15, 16, 17, 18, 19, and 20, respectively.

[0068] In some embodiments, the pterostilbene reductase is one or more of GgPTR1, GgPTR2, GgPTR3, GgPTR4, GgPTR5, GgPTR6, GgPTR7, and GgPTR8 from Glycyrrhiza glabra, and their amino acid sequences are shown in SEQ ID NOs: 21, 22, 23, 24, 25, 26, 27, and 28, respectively.

[0069] In some embodiments, the oxidative cyclase is one or more of GgOC1, GgOC2, GgOC3, GgOC4, GgOC5, GgOC6, GgOC7, and GgOC8 from Glycyrrhiza glabra, and their amino acid sequences are shown in SEQ ID NOs: 29, 30, 31, 32, 33, 34, 35, and 36, respectively.

[0070] In some embodiments, the demethylase is derived from one or more of Fusarium solani, Glycyrrhiza glabra, Glycyrrhiza uralensis, and Glycyrrhiza inflata, wherein the demethylase derived from Fusarium solani is NhDMT, and its amino acid sequence is shown in SEQ ID NO: 37; the demethylase derived from Glycyrrhiza glabra is one or more of GgDMT1, GgDMT2, GgDMT3, GgDMT4, GgDMT5, GgDMT6, GgDMT7, and GgDMT8, and their amino acid sequences are shown in SEQ ID NOs: 38, 39, 40, 41, 42, 43, 44, and 45, respectively; the demethylase derived from Glycyrrhiza uralensis is one or more of GuDMT1, GuDMT2, and GuDMT3, and their amino acid sequences are shown in SEQ ID NOs: As shown in NO:46, 47, and 48, the demethylase derived from Glycyrrhiza inflata is one or more of GiDMT1, GiDMT2, and GiDMT3, and their amino acid sequences are shown in SEQ ID NO:49, 50, and 51, respectively.

[0071] In some embodiments, the cytochrome P450 reductase is GuCPR2 from Glycyrrhiza uralensis, whose amino acid sequence is shown in SEQ ID NO:52.

[0072] In some embodiments, the engineered biological host heterologously expresses: phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SetAL from Saccharothrix hispanica, p-coumarate Coenzyme A ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase from Glycyrrhiza glabra GgIFS, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase tPcM4DT from Psoralea corylifolia, pterostilbene reductase GgPTR1 from Glycyrrhiza glabra, oxidative cyclase GgOC from Glycyrrhiza glabra, demethylase NhDMT from Fusarium solani (corresponding to GLA1 in the examples of the present application); or

[0073] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SetAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, isoprenyltransferase GgPT from Glycyrrhiza glabra, pterostilbene reductase GgPTR2 from Glycyrrhiza glabra, oxidative cyclase GgOC from Glycyrrhiza glabra, demethylase GgDMT1 from Glycyrrhiza glabra (corresponding to GLA2 in the examples of the present application); or

[0074] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT2 from Glycyrrhiza glabra, pterostilbene reductase GgPTR3 from Glycyrrhiza glabra, oxidative cyclase GgOC3 from Glycyrrhiza glabra, demethylase GgDMT2 from Glycyrrhiza glabra (corresponding to GLA3 in the examples of the present application); or

[0075] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, isoprenyltransferase GgPT3 from Glycyrrhiza glabra, pterostilbene reductase GgPTR4 from Glycyrrhiza glabra, oxidative cyclase GgOC4 from Glycyrrhiza glabra, demethylase GgDMT3 from Glycyrrhiza glabra (corresponding to GLA4 in the examples of the present application); or

[0076] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT4 from Glycyrrhiza glabra, pterostilbene reductase GgPTR5 from Glycyrrhiza glabra, oxidative cyclase GgOC5 from Glycyrrhiza glabra, demethylase GgDMT4 from Glycyrrhiza glabra (corresponding to GLA5 in the examples of the present application); or

[0077] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT5 from Glycyrrhiza glabra, pterostilbene reductase GgPTR6 from Glycyrrhiza glabra, oxidative cyclase GgOC6 from Glycyrrhiza glabra, demethylase GgDMT5 from Glycyrrhiza glabra (corresponding to GLA6 in the examples of the present application); or

[0078] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT6 from Glycyrrhiza glabra, pterostilbene reductase GgPTR7 from Glycyrrhiza glabra, oxidative cyclase GgOC7 from Glycyrrhiza glabra, demethylase GgDMT6 from Glycyrrhiza glabra (corresponding to GLA7 in the examples of the present application); or

[0079] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT7 from Glycyrrhiza glabra, pterostilbene reductase GgPTR8 from Glycyrrhiza glabra, oxidative cyclase GgOC8 from Glycyrrhiza glabra, demethylase GgDMT7 from Glycyrrhiza glabra (corresponding to GLA8 in the examples of the present application); or

[0080] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT1 from Glycyrrhiza glabra, pterostilbene reductase GgPTR2 from Glycyrrhiza glabra, oxidative cyclase GgOC2 from Glycyrrhiza glabra, demethylase GgDMT8 from Glycyrrhiza glabra (corresponding to GLA9 in the examples of the present application); or

[0081] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT2 from Glycyrrhiza glabra, pterostilbene reductase GgPTR3 from Glycyrrhiza glabra, oxidative cyclase GgOC3 from Glycyrrhiza glabra, demethylase GuDMT1 from Glycyrrhiza glabra (corresponding to GLA10 in the examples of the present application); or

[0082] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, isoprenyltransferase GgPT3 from Glycyrrhiza glabra, pterostilbene reductase GgPTR4 from Glycyrrhiza glabra, oxidative cyclase GgOC4 from Glycyrrhiza glabra, demethylase GuDMT2 from Glycyrrhiza glabra (corresponding to GLA11 in the examples of the present application); or

[0083] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT4 from Glycyrrhiza glabra, pterostilbene reductase GgPTR5 from Glycyrrhiza glabra, oxidative cyclase GgOC5 from Glycyrrhiza glabra, demethylase GuDMT3 from Glycyrrhiza glabra (corresponding to GLA12 in the examples of the present application); or

[0084] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT5 from Glycyrrhiza glabra, pterostilbene reductase GgPTR6 from Glycyrrhiza glabra, oxidative cyclase GgOC6 from Glycyrrhiza glabra, demethylase GiDMT1 from Glycyrrhiza glabra (corresponding to GLA13 in the examples of the present application); or

[0085] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT6 from Glycyrrhiza glabra, pterostilbene reductase GgPTR7 from Glycyrrhiza glabra, oxidative cyclase GgOC7 from Glycyrrhiza glabra, demethylase GiDMT2 from Glycyrrhiza glabra (corresponding to GLA14 in the examples of the present application); or

[0086] Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SetAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, isoprenyltransferase GgPT7 from Glycyrrhiza glabra, pterostilbene reductase GgPTR8 from Glycyrrhiza glabra, oxidative cyclase GgOC8 from Glycyrrhiza glabra, demethylase GiDMT3 from Glycyrrhiza glabra (corresponding to GLA15 in the examples of the present application).

[0087] In some embodiments, the biological host comprises: GLA1, GLA2, GLA3, GLA4, GLA9, GLA10, GLA11.

[0088] In some embodiments, the engineered host organism expresses a mutated 4'-O-methyltransferase from kudzu comprising one or more of the following mutations: F24Y, F307L, F307I, F307V, M311L, Y160P, and Y160L, wherein the amino acid sequence of the 4'-O-methyltransferase from kudzu has the accession number AKW47171.1 in the NCBI database.

[0089] In some embodiments, the modified biological host expresses: a mutated ARO4 from Saccharomyces cerevisiae comprising the following mutation: K229L; and / or a mutated ARO7 from Saccharomyces cerevisiae comprising the following mutation: G141S, wherein the accession number of ARO4 from Saccharomyces cerevisiae in the NCBI database is NP_009808.1, and the accession number of ARO7 from Saccharomyces cerevisiae in the NCBI database is NP_015385.1.

[0090] In some embodiments, the engineered biological host heterologously expresses or overexpresses: a dimethylallyl pyrophosphate synthase, wherein the dimethylallyl pyrophosphate synthesis gene is selected from the group consisting of: acetyl-CoA acetyltransferase, 3-hydroxy-3-methylglutaryl-CoA synthase, hydroxymethylglutaryl-CoA reductase, mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylase, and dimethylallyl diphosphate isomerase.

[0091] In some embodiments, the modified biological host heterologously expresses or overexpresses an enzyme selected from the group consisting of: 6-phosphate glucose dehydrogenase, malate dehydrogenase, and NADH-dependent hydroxymethylglutaryl-CoA reductase, wherein the accession number of the NCBI database for the 6-phosphate glucose dehydrogenase is NP_014158.1, the accession number of the NCBI database for the malate dehydrogenase is NP_012896.1, and the accession number of the NADH-dependent hydroxymethylglutaryl-CoA reductase is WP_011241944.1.

[0092] In some embodiments, the engineered host organism heterologously expresses or overexpresses acetyl-CoA carboxylase.

[0093] In some embodiments, the engineered host organism expresses the demethylase using a galactose-inducible promoter or a xylose-inducible promoter.

[0094] In some embodiments, the biological host is selected from the group consisting of Gram-positive bacteria, Gram-negative bacteria, actinomycetes, yeast, filamentous fungi, and tobacco.

[0095] In some embodiments, the Gram-positive bacteria include Bacillus subtilis and Corynebacterium glutamicum, the Gram-negative bacteria include Escherichia coli, the actinomycetes include Streptomyces coelicolor, the yeast include Saccharomyces cerevisiae, Yarrowia lipolytica, and Pichia pastoris, the filamentous fungi include Aspergillus niger, and the tobacco includes Nicotiana benthamiana.

[0096] In some embodiments, the engineered biological host is Saccharomyces cerevisiae Glabd-831 with a deposit number of CGMCC No. 33965.

[0097] The present application provides an engineered brewer's yeast, namely GLA2-5 of the present application example, which is named Saccharomyces cerevisiae Glabd-831 and deposited; the deposit registration number is CGMCC No. 33965, the depository institution is: China General Microbiological Culture Collection Center; the depository address is: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the postal code is 100101; the deposit date is March 24, 2025.

[0098] Compared with the related art, the embodiments of the present invention achieve at least the following beneficial effects:

[0099] The present invention utilizes metabolic engineering and synthetic biology to introduce phenylalanine ammonia lyase, cinnamate 4-hydrogenase, tyrosine ammonia lyase, p-coumarate CoA ligase, chalcone synthase, chalcone reductase, chalcone isomerase, isoflavone synthase, 2-hydroxyisoflavone dehydratase, 4'-oxymethyltransferase, oxygen methyltransferase, isoflavone 2'-hydrogenase, isoflavone reductase, visnezyl reductase, prenyltransferase, pterostilbene reductase, oxidative cyclase, demethylase, and cytochrome P450 reductase into Saccharomyces cerevisiae, achieving efficient de novo synthesis of glabridin. The engineered yeast constructed by the present invention makes the production process of glabridin simple, rapid, and environmentally friendly, and is a potential method for achieving large-scale industrial production of glabridin. BRIEF DESCRIPTION OF THE DRAWINGS

[0100] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0101] Figure 1 Schematic flow chart of the de novo synthesis of plant-derived glabridin in Saccharomyces cerevisiae using glucose as substrate. DETAILED DESCRIPTION

[0102] Below by the specific embodiment of synthetic glabridin, the present invention is further described, the purpose of specific embodiment is just for illustration, is not used to limit the scope of the present invention.Under the condition that does not deviate from the scope of claims, those skilled in the art can make modifications to various aspects of the present invention, but these modifications also belong to protection scope of the present invention.For example, the cell expression system and the vector plasmid used in the present embodiment are replaced with other expression systems and carriers commonly used in this area, and other persons skilled in the art can understand and realize.

[0103] The present invention mines a series of enzymes related to the biosynthesis of glabridin from Glycyrrhiza glabra, and sequentially introduces phenylalanine ammonia lyase, cinnamate-4-hydrogenase, tyrosine ammonia lyase, p-coumarate Coenzyme A ligase, chalcone synthase, chalcone reductase, chalcone isomerase, isoflavone synthase, 2-hydroxyisoflavone dehydratase, 4'-oxymethyltransferase, oxygen methyltransferase, isoflavone 2'-hydrogenase, isoflavone reductase, visnezone reductase, prenyltransferase, pterostilbene reductase, oxidative cyclase, demethylase, cytochrome P450 reductase and the like into Saccharomyces cerevisiae, thereby realizing the de novo synthesis of plant-derived glabridin (attached) in Saccharomyces cerevisiae using glucose as a substrate. Figure 1 In particular, the present invention combines enhanced precursor supply with enzyme engineering and other design approaches. Using glucose as the raw material, through batch fed-batch fermentation, engineered yeast can efficiently synthesize more than 95 mg / L of glabridin, achieving the first de novo synthesis of glabridin in a microbial cell factory.

[0104] The present invention provides a method for constructing an engineered yeast for producing glabridin, comprising the following steps:

[0105] 1. Construction of expression cassette:

[0106] The left homology arm, yeast promoter, gene, and yeast terminator were connected using overlap extension PCR to obtain the relevant expression cassette;

[0107] 2. Construction of screening marker expression cassette:

[0108] The yeast terminator, leucine expression cassette gene P Leu2 -leu2-T Leu2 , the right homology arms were connected using overlap extension PCR to obtain a selection marker expression cassette;

[0109] 3. Insert the expression cassette of the above gene and the selection marker expression cassette into the yeast genome or plasmid to obtain an engineered yeast that produces glabridin.

[0110] the term

[0111] As used herein, the "engineered bacteria" or "engineered plants" refer to fungal cell lines or plants in which exogenous genes are efficiently expressed using genetic engineering methods.

[0112] As used herein, the "starting strain" and "chassis strain" refer to the original strain used for transformation.

[0113] As used herein, the "gene circuit" refers to a gene expression system that contains all the necessary elements required to express a target polypeptide, generally including the following elements: a promoter, an encoded reporter gene, and a terminator.

[0114] The terms "comprising" or "including" as used in the present invention are open-ended. When used to describe a protein or nucleic acid sequence, the protein or nucleic acid may be composed of the sequence, or may have additional amino acids or nucleotides at one or both ends of the protein or nucleic acid, but still have the same or similar activity as the original sequence.

[0115] The terms “include” or “comprising” described in the present invention are open-ended descriptions, which contain the specified components or steps described and other specified components or steps that will not be substantially affected.

[0116] The term "and / or" as used herein includes all combinations of the items connected by the term, and each combination should be deemed to have been listed separately herein. For example, "A and / or B" includes "A," "A and B," and "B." For another example, "A, B, and / or C" includes "A," "B," "C," "A and B," "A and C," "B and C," and "A and B and C."

[0117] The present invention will be further explained below by way of specific examples. It should be understood that these examples are merely provided to illustrate and help understand the present invention, and are not intended to limit the present invention to these examples.

[0118] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0119] Example 1 Obtaining Related Genes in the Glabridin Synthesis Pathway

[0120] According to the amino acid sequence of claim 1, after codon optimization, gene synthesis was performed: (1) phenylalanine ammonia lyase genes MtPAL (SEQ ID NO: 53) and GgPAL (SEQ ID NO: 54); (2) cinnamate-4-hydrogenase genes AtC4H (SEQ ID NO: 55) and GgC4H (SEQ ID NO: 56); (3) tyrosine ammonia lyase gene SeTAL (SEQ ID NO: 57); (4) 4-coumaroyl-CoA ligase genes Pc4CL (SEQ ID NO: 58) and Gg4CL (SEQ ID NO: 59); (5) chalcone synthase genes PhCHS (SEQ ID NO: 60) and GgCHS (SEQ ID NO: 61); (6) chalcone reductase gene GgCHR (SEQ ID NO: 62); (7) chalcone isomerase genes MsCHI (SEQ ID NO: 63) and GgCHI (SEQ ID NO: 64). NO:64) and AtCHIL (SEQ ID NO:65); (8) isoflavone synthase gene GgIFS (SEQ ID NO:66); (9) 2-hydroxyisoflavone dehydratase gene GgHID (SEQ ID NO:67); (10) 4'-O-methyltransferase genes P1OMT9 (SEQ ID NO:68) and GgOMT (SEQ ID NO:69); (11) isoflavone 2'-hydrogenase genes GeCYP81E1 (SEQ ID NO:70) and GgI2'H (SEQ ID NO:71); (12) isoflavone reductase genes MsIFR (SEQ ID NO:72) and GgIFR (SEQ ID NO:73); (13) visnezyl reductase genes MsVR (SEQ ID NO:74) and GgVR (SEQ ID NO:75); (14) prenyltransferase gene tPcM4DT (SEQ ID NO:76). NO:76), GgPT1 (SEQ ID NO:77), GgPT2 (SEQ ID NO:78), GgPT3 (SEQ ID NO:79), GgPT4 (SEQ ID NO:80), GgPT5 (SEQ ID NO:81), GgPT6 (SEQ ID NO:82), GgPT7 (SEQ ID NO:83);(15) Pterostilbene reductase genes GgPTR1 (SEQ ID NO: 84), GgPTR2 (SEQ ID NO: 85), GgPTR3 (SEQ ID NO: 86), GgPTR4 (SEQ ID NO: 87), GgPTR5 (SEQ ID NO: 88), GgPTR6 (SEQ ID NO: 89), GgPTR7 (SEQ ID NO: 90), GgPTR8 (SEQ ID NO: 91); (16) Oxidative cyclase genes GgOC1 (SEQ ID NO: 92), GgOC2 (SEQ ID NO: 93), GgOC3 (SEQ ID NO: 94), GgOC4 (SEQ ID NO: 95), GgOC5 (SEQ ID NO: 96), GgOC6 (SEQ ID NO: 97), GgOC7 (SEQ ID NO: 98), GgOC8 (SEQ ID NO: 99); (17) Demethylase gene NhDMT (SEQ ID NO: 91). NO:100), GgDMT1 (SEQ ID NO:101), GgDMT2 (SEQ ID NO:102), GgDMT3 (SEQ ID NO:103), GgDMT4 (SEQ ID NO:104), GgDMT5 (SEQ ID NO:105), GgDMT6 (SEQ ID NO:106), GgDMT7 (SEQ ID NO:107), GgDMT8 (SEQ ID NO:108), GuDMT1 (SEQ ID NO:109), GuDMT2 (SEQ ID NO:110), GuDMT3 (SEQ ID NO:111), GiDMT1 (SEQ ID NO:112), GiDMT2 (SEQ ID NO:113), GiDMT3 (SEQ ID NO: 114); (18) Cytochrome P450 reductase gene GuCPR2 (SEQ ID NO:115); (19) selection marker gene expression cassettes LEU2 (derived from plasmid pRS415, a conventional plasmid, sequence source: www.snapgene.com / plasmids / yeast_plasmids / pRS415), URA3 (derived from plasmid pRS416, a conventional plasmid, sequence source: www.snapgene.com / plasmids / yeast_plasmids / pRS416), and HIS3 (derived from plasmid pRS413, a conventional plasmid, sequence source: www.snapgene.com / plasmids / yeast_plasmids / pRS413);(20) Homology arms for integration into the Saccharomyces cerevisiae genome (integration site: YPRCtau3, primers for amplifying the upstream homology arm YPRC3L sequence: 5'-CAGTGAATTCGAAAAGGAGGTGCACGCATTAT-3' (SEQ ID NO: 116) and 5'-AGGAGAAAAAACTTCCAAGGAGGTGAAGAACGTC-3' (SEQ ID NO: 117), primers for the downstream homology arm YPRC3R: 5'-GATATTGGAGGGATGGGACGTCAGCACTG-3' (SEQ ID NO: 118) and 5'-CTCTAGAGGATCCCCGGGTACGGTATTACTCGAGCCCG-3' (SEQ ID NO: 119)).

[0121] The above DNA was amplified by PCR using DNA polymerase Phanta Max Super-Fidelity DNA Polymerase (purchased from Novagen) and cloned into the pUC19 vector (conventional plasmid, sequence source: https: / / www.snapgene.com / plasmids / basic_cloning_vectors / pUC19). Sequencing confirmed that it had no mutation.

[0122] Example 2 Construction and Verification of the Liquoricein Synthesis Module in the Glabridin Synthesis Pathway

[0123] In order to realize the de novo synthesis of glycyrrhizin in Saccharomyces cerevisiae, the synthesis pathway was divided into four modules and constructed separately, namely: (1) glycyrrhizin synthesis module; (2) daidzein synthesis module; (3) meditartin synthesis module; and (4) glycyrrhizin synthesis module.

[0124] The gene fragments in the example were connected into the following two gene lines by OE-PCR and Gibson assembly: ① YPRC3L-P PGI1 -MtPAL-T GPD1 -P ENO1 -AtC4H-T MDH2 -P TEF1 -SeTAL-T CYC1 -P TPI1 -Pc4CL-T HHF2 -P GPM1 -PhCHS-T ALD6 -P GPP1 -GgCHR-T LSC1 -P PDC1 -MsCHI-T DLD1 -P MDH1 -GuCPR2-TSGA1 -LEU2-YPRC3R;②YPRC3L-P PGI1 -GgPAL-T GPD1 -P ENO1 -GgC4H-T MDH2 -P TEF1 -SeTAL-T CYC1 -P TPI1 -Gg4CL-T HHF2 -P GPM1 -GgCHS-T ALD6 -P GPP1 -GgCHR-T LSC1 -P PDC1 -GgCHI-T DLD1 -P MDH1 -GuCPR2-T SGA1 -LEU2-YPRC3R. The promoter and terminator used are commonly used elements.

[0125] The two gene lines described above were transformed into Saccharomyces cerevisiae BY4741 (a common strain in the S288 line, Cohen R, Engelberg D. Commonly used Saccharomyces cerevisiae strains (e.g., BY4741, W303) are growth sensitive on synthetic complete medium due to poor leucine uptake. FEMS Microbiol Lett. 2007, 273(2):239-43) using lithium acetate transformation. The transformed bacteria were plated on SD-LEU solid medium and cultured at 30°C for 3 days. Positive clones were identified by colony PCR. Strains containing the corresponding two gene lines were named LIQ1 and LIQ2, respectively.

[0126] Two strains were fermented in YPD medium at 30°C and 200 rpm for 5 days. After fermentation, the fermentation broth was bead-beaten and extracted with an equal volume of ethyl acetate. The resulting organic phase was evaporated to dryness and reconstituted with methanol. Liquoricerin content was determined by ultra-performance liquid chromatography (UPLC) or liquid chromatography-mass spectrometry (LC-MS). The fermentation products of the engineered strains were quantitatively determined using an Agilent 1260 UPLC system. A Poroshell 120EC-C18 column with a 2.7 μm particle size and a 3.0 × 100 mm diameter was used. The detection wavelength was 254 nm, the column oven was 35°C, and the mobile phase A was 0.1% formic acid in water; the mobile phase B was acetonitrile. The gradient elution profile was: 0 min, 20% B; 0–4.5 min, 20%–35% B; 4.5–6 min, 35%–50% B; 6–12 min, 50%–65% B; 12–18 min, 65%–85% B; 18–22.5 min, 85%–95% B; 22.5–24 min, 95%–20% B; and 24–30 min, 20% B. The flow rate was 0.2 mL / min, and the injection volume was 1 μL. External standard methods were used for qualitative and quantitative determination. The results showed that strains LIQ1 and LIQ2 produced 51 mg / L and 198 mg / L of liquiritigenin, respectively. Therefore, strain LIQ2 was selected as the basis for subsequent strain construction.

[0127] Example 3 Construction and verification of the formononetin synthesis module in the glabridin synthesis pathway

[0128] The gene fragments in the embodiment were connected into the following gene circuits by OE-PCR and Gibson assembly: ①T SGA1 -P CIT1 -GgIFS-T COX8 -P HHF1 -GgHID-T RPS31 -P TDH3 -PlOMT9-T LYS5 -URA3-YPRC3R,②T SGA1 -P CIT1 -GgIFS-T COX8 -P HHF1 -GgHID-T RPS31 -P TDH3 -GgOMT-T LYS5 -URA3-YPRC3R. The promoter and terminator used are commonly used elements.

[0129] The above-mentioned gene circuit was transformed into strain LIQ2 using lithium acetate transformation. The transformed bacteria were plated on SD-URA solid medium and cultured at 30°C for 3 days. Positive clones were identified by colony PCR. These clones were named FOR1 and FOR2. The strains were fermented in YPD medium on a shaker at 30°C and 200 rpm for 5 days. After fermentation, the fermentation broth was bead-beated and extracted with an equal volume of ethyl acetate. The resulting organic phase was evaporated to dryness and reconstituted with methanol. Daidzein content was determined by ultra-performance liquid chromatography (UPLC) or liquid chromatography-mass spectrometry (LC-MS). Fermentation products of the engineered strains were quantitatively determined using an Agilent 1260 UPLC system. A Poroshell 120EC-C18 column with a particle size of 2.7 μm and a diameter of 3.0 × 100 mm was used. The detection wavelength was 254 nm, and the column oven was set at 35°C. Mobile phase A consisted of 0.1% formic acid in water and mobile phase B consisted of acetonitrile. The gradient elution profile was: 0 min, 20% B; 0–4.5 min, 20%–35% B; 4.5–6 min, 35%–50% B; 6–12 min, 50%–65% B; 12–18 min, 65%–85% B; 18–22.5 min, 85%–95% B; 22.5–24 min, 95%–20% B; and 24–30 min, 20% B. The flow rate was 0.2 mL / min, and the injection volume was 1 μL. External standard analysis was used for qualitative and quantitative determination. The results showed that strains FOR1 and FOR2 produced 15 mg / L and 16 mg / L of formononetin, respectively.

[0130] Example 4 Construction and verification of the synthesis module of medipterostilbene in the synthesis pathway of glabridin

[0131] The gene fragments in the embodiment were connected into the following two gene circuits by OE-PCR and Gibson assembly: ①T LYS5 -P COX4 -GeCYP81E1-T DPM1 -P PCK1 -MsIFR-T ADE8 -P CIT2 -MsVR-T PLC1 -LEU2-YPRC3R;②T LYS5 -P COX4 -GgI2'HT DPM1 -P PCK1 -GgIFR-T ADE8 -P CIT2 -GgVR-T PLC1 -LEU2-YPRC3R. The promoter and terminator used are commonly used elements.

[0132] The two gene circuits were transformed into strain FOR2 using lithium acetate. The transformed bacteria were plated on SD-LEU solid medium and cultured at 30°C for 3 days. Positive clones were identified by colony PCR. The strains containing the corresponding two gene circuits were named MED1 and MED2, respectively.

[0133] The two strains were fermented in YPD medium at 30°C and 200 rpm for 5 days. After fermentation, the fermentation broth was bead-beaten and extracted with an equal volume of ethyl acetate. The resulting organic phase was evaporated to dryness and reconstituted with methanol. Meditartin content was determined by ultra-performance liquid chromatography (UPLC) or liquid chromatography-mass spectrometry (LC-MS). The fermentation products of the engineered strains were quantitatively determined using an Agilent 1260 UPLC instrument. A Poroshell 120EC-C18 column with a particle size of 2.7 μm and a 3.0 × 100 mm diameter was used. The detection wavelength was 254 nm, the column oven was set at 35°C, and the mobile phase A was 0.1% formic acid in water; the mobile phase B was acetonitrile. The gradient elution profile was: 0 min, 20% B; 0–4.5 min, 20%–35% B; 4.5–6 min, 35%–50% B; 6–12 min, 50%–65% B; 12–18 min, 65%–85% B; 18–22.5 min, 85%–95% B; 22.5–24 min, 95%–20% B; and 24–30 min, 20% B. The flow rate was 0.2 mL / min, and the injection volume was 1 μL. External standard methods were used for qualitative and quantitative determination. The results showed that strains MED1 and MED2 could produce 5 mg / L and 16 mg / L of meditartin, respectively. Therefore, strain MED2 was selected as the basis for subsequent strain construction.

[0134] Example 5 Construction and Verification of the Glabridin Synthesis Module in the Glabridin Synthesis Pathway

[0135] The gene fragments in the example were connected into the following 15 gene circuits by OE-PCR and Gibson assembly: (1) T PLC1 -P TEF2 -tPcM4DT-T RPN9 -P PGK1 -GgPTR1-T TYC1 -P TDH1 -GgOC1-T TFA2 -P HSP26 -NhDMT-T CPP3 -URA3-YPRC3R;(2)T PLC1 -P TEF2 -GgPT1-T RPN9 -P PGK1 -GgPTR2-TTYC1 -P TDH1 -GgOC2-T TFA2 -P HSP26 -GgDMT1-T CPP3 -URA3-YPRC3R;(3)T PLC1 -P TEF2 -GgPT2-T RPN9 -P PGK1 -GgPTR3-T TYC1 -P TDH1 -GgOC3-T TFA2 -P HSP26 -GgDMT2-T CPP3 -URA3-YPRC3R;(4)T PLC1 -P TEF2 -GgPT3-T RPN9 -P PGK1 -GgPTR4-T TYC1 -P TDH1 -GgOC4-T TFA2 -P HSP26 -GgDMT3-T CPP3 -URA3-YPRC3R;(5)T PLC1 -P TEF2 -GgPT4-T RPN9 -P PGK1 -GgPTR5-T TYC1 -P TDH1 -GgOC5-T TFA2 -P HSP26 -GgDMT4-T CPP3 -URA3-YPRC3R;(6)T PLC1 -P TEF2 -GgPT5-T RPN9 -P PGK1 -GgPTR6-T TYC1 -P TDH1 -GgOC6-T TFA2 -P HSP26 -GgDMT5-T CPP3 -URA3-YPRC3R;(7)T PLC1 -P TEF2 -GgPT6-T RPN9 -P PGK1 -GgPTR7-T TYC1 -P TDH1 -GgOC7-T TFA2 -P HSP26 -GgDMT6-T CPP3 -URA3-YPRC3R;(8)T PLC1 -PTEF2 -GgPT7-T RPN9 -P PGK1 -GgPTR8-T TYC1 -P TDH1 -GgOC8-T TFA2 -P HSP26 -GgDMT7-T CPP3 -URA3-YPRC3R;(9)T PLC1 -P TEF2 -GgPT1-T RPN9 -P PGK1 -GgPTR2-T TYC1 -P TDH1 -GgOC2-T TFA2 -P HSP26 -GgDMT8-T CPP3 -URA3-YPRC3R;(10)T PLC1 -P TEF2 -GgPT2-T RPN9 -P PGK1 -GgPTR3-T TYC1 -P TDH1 -GgOC3-T TFA2 -P HSP26 -GuDMT1-T CPP3 -URA3-YPRC3R;(11)T PLC1 -P TEF2 -GgPT3-T RPN9 -P PGK1 -GgPTR4-T TYC1 -P TDH1 -GgOC4-T TFA2 -P HSP26 -GuDMT2-T CPP3 -URA3-YPRC3R;(12)T PLC1 -P TEF2 -GgPT4-T RPN9 -P PGK1 -GgPTR5-T TYC1 -P TDH1 -GgOC5-T TFA2 -P HSP26 -GuDMT3-T CPP3 -URA3-YPRC3R;(13)T PLC1 -P TEF2 -GgPT5-T RPN9 -P PGK1 -GgPTR6-T TYC1 -P TDH1 -GgOC6-T TFA2 -PHSP26 -GiDMT1-T CPP3 -URA3-YPRC3R;(14)T PLC1 -P TEF2 -GgPT6-T RPN9 -P PGK1 -GgPTR7-T TYC1 -P TDH1 -GgOC7-T TFA2 -P HSP26 -GiDMT2-T CPP3 -URA3-YPRC3R;(15)T PLC1 -P TEF2 -GgPT7-T RPN9 -P PGK1 -GgPTR8-T TYC1 -P TDH1 -GgOC8-T TFA2 -P HSP26 -GiDMT3-T CPP3 -URA3-YPRC3R. The promoter and terminator used are commonly used elements.

[0136] The two gene circuits were transformed into strain MED2 using lithium acetate. The transformed bacteria were plated on SD-URA solid medium and cultured at 30°C for 3 days. Positive clones were identified by colony PCR. The strains containing the corresponding 15 gene circuits were named GLA1, GLA2, GLA3, GLA4, GLA5, GLA6, GLA7, GLA8, GLA9, GLA10, GLA11, GLA12, GLA13, GLA14, and GLA15.

[0137] Two strains were fermented in YPD medium at 30°C and 200 rpm for 5 days. After fermentation, the fermentation broth was bead-beaten and extracted with an equal volume of ethyl acetate. The resulting organic phase was evaporated to dryness and reconstituted with methanol. Glabridin content was determined by ultra-performance liquid chromatography (UPLC) or liquid chromatography-mass spectrometry (LC-MS). Fermentation products of the engineered strains were quantitatively determined using an Agilent 1260 UPLC system. A Poroshell 120EC-C18 column with a 2.7 μm particle size and a 3.0 × 100 mm diameter was used. The detection wavelength was 254 nm, and the column oven was set at 35°C. Mobile phase A consisted of 0.1% formic acid in water and mobile phase B consisted of acetonitrile. The gradient elution profile was as follows: 0 min, 20% B; 0–4.5 min, 20%–35% B; 4.5–6 min, 35%–50% B; 6–12 min, 50%–65% B; 12–18 min, 65%–85% B; 18–22.5 min, 85%–95% B; 22.5–24 min, 95%–20% B; and 24–30 min, 20% B. The flow rate was 0.2 mL / min, and the injection volume was 1 μL. External standards were used for qualitative and quantitative determination. The test results showed that strains GLA1, GLA2, GLA3, GLA4, GLA5, GLA6, GLA7, GLA8, GLA9, GLA10, GLA11, GLA12, GLA13, GLA14, and GLA15 could produce 2.4 mg / L, 4 mg / L, 3.9 mg / L, 3.9 mg / L, 1.5 mg / L, 1.4 mg / L, 0.3 mg / L, 0.1 mg / L, 3.8 mg / L, 3.6 mg / L, 3.5 mg / L, 1.4 mg / L, 1.3 mg / L, 0.4 mg / L, and 0.2 mg / L of glabridin, respectively.

[0138] Example 6 Construction and Verification of P10MT9 Mutants in Glabridin Synthetic Strain

[0139] To improve the catalytic activity and specificity of 4'-O-methyltransferase, the enzyme structure of P10MT9 was rationally designed. Through structural simulation, molecular docking and kinetic simulation, four key amino acid residues affecting enzyme activity, F24, F307, M311 and Y160, were obtained. Primers were designed to perform saturation mutagenesis on the four sites respectively, and the residues were constructed into expression cassettes to obtain a series of gene circuits P10MT9. TDH3 -PlOMT9mutant-T LYS5 -HIS3-P COX4 The above gene circuit was transformed into strain GLA2 by lithium acetate transformation. The transformed bacteria were spread on SD-HIS solid medium and cultured at 30°C for 3 days. Positive clones were obtained by colony PCR verification.

[0140] The strain was fermented in YPD medium at 30°C and 200 rpm for 5 days. After fermentation, the fermentation broth was bead-beaten and extracted with an equal volume of ethyl acetate. The resulting organic phase was evaporated to dryness and reconstituted with methanol. Glabridin content was determined by ultra-performance liquid chromatography (UPLC) or liquid chromatography-mass spectrometry (LC-MS). Fermentation products of the engineered strain were quantitatively determined using an Agilent 1260 UPLC system. A Poroshell 120EC-C18 column with a particle size of 2.7 μm and a 3.0 × 100 mm diameter was used. The detection wavelength was 254 nm, the column oven was at 35°C, mobile phase A was 0.1% formic acid in water, mobile phase B was acetonitrile, and the gradient elution process was: 0 min, 20% B; 0–4.5 min, 20%–35% B; 4.5–6 min, 35%–50% B; 6–12 min, 50%–65% B; 12–18 min, 65%–85% B; 18–22.5 min, 85%–95% B; 22.5–24 min, 95%–20% B; and 24–30 min, 20% B. The flow rate was 0.2 mL / min, and the injection volume was 1 μL. Qualitative and quantitative determinations were performed using an external standard method. The results indicated that mutant P1OMT9 F24Y PlOMT9 F307L PlOMT9 F307I PlOMT9 F307V PlOMT9 M311L PlOMT9 Y160P PlOMT9 Y160L The concentrations of glabridin produced were 4.3 mg / L, 4.8 mg / L, 5.1 mg / L, 4.8 mg / L, 4.3 mg / L, 4.9 mg / L, and 4.2 mg / L, respectively. The results showed that the modification of the above mutants could improve the synthesis efficiency of glabridin compared with the control strain GLA2.

[0141] Example 7 Enhancement and Verification of Amino Acid Supply in Glabridin Synthetic Strain

[0142] Genes ARO4 and ARO7 in Saccharomyces cerevisiae BY4741 were amplified by PCR, and mutants of the two genes were obtained using the OE-PCR method. K229L and ARO7 G141S . The following gene circuits were further constructed: T CPP3 -P ATP1 -ARO4 K229L -T PST1 -P ATP5 -ARO7G141S -T PRB1 -LEU2-YPRC3R. The promoter and terminator used were commonly used elements. The above gene circuit was transformed into the strain GLA2 using lithium acetate transformation. The transformed bacteria were plated on SD-LEU solid medium and cultured at 30°C for 3 days. Positive clones were identified by colony PCR. The strain was named GLA2-1.

[0143] The strain was fermented in YPD medium at 30°C and 200 rpm for 5 days. After fermentation, the fermentation broth was bead-beaten and extracted with an equal volume of ethyl acetate. The resulting organic phase was evaporated to dryness and reconstituted with methanol. Glabridin content was determined by ultra-performance liquid chromatography (UPLC) or liquid chromatography-mass spectrometry (LC-MS). Fermentation products of the engineered strain were quantitatively determined using an Agilent 1260 UPLC system. A Poroshell 120EC-C18 column with a particle size of 2.7 μm and a 3.0 × 100 mm diameter was used. The detection wavelength was 254 nm, the column oven was 35°C, and the mobile phase A was 0.1% formic acid in water. The mobile phase B was acetonitrile. The gradient elution profile was: 0 min, 20% B; 0–4.5 min, 20%–35% B; 4.5–6 min, 35%–50% B; 6–12 min, 50%–65% B; 12–18 min, 65%–85% B; 18–22.5 min, 85%–95% B; 22.5–24 min, 95%–20% B; and 24–30 min, 20% B. The flow rate was 0.2 mL / min, and the injection volume was 1 μL. External standard methods were used for qualitative and quantitative determination. The results showed that strain GLA2-1 produced 5.3 mg / L of glabridin.

[0144] Example 8 Enhancement and Verification of Isoprene Donors in Glabridin Synthetic Strain

[0145] Genes ERG10, ERG13, tHMG1, ERG12, ERG8, MVD1, IDI1, and ERG20 from Saccharomyces cerevisiae BY4741 were amplified by PCR. OE-PCR and Gibson assembly were then used to construct the following gene circuits: PRB1 -P RPS11A -ERG10-T TIM22 -P RPS20 -ERG13-T SGE1 -P RPS17A -tHMG1-T SOL1 -P RPS18A -ERG12-T SPC2 -P TSA1-ERG8-T PEP1 -P RPS10B -MVD1-T PFD1 -P RPL41B -IDI1-T RTG3 -URA3-YPRC3R and MVA pathways dynamically regulate gene circuits: ERG20-P HXT1 -ERG20.

[0146] The promoters and terminators used were commonly used. The above gene circuit was transformed into strain GLA2-1 using lithium acetate transformation. The transformed bacteria were plated on SD-URA solid medium and cultured at 30°C for 3 days. Positive clones were identified by colony PCR. The strain was named GLA2-2.

[0147] The strain was fermented in YPD medium at 30°C and 200 rpm for 5 days. After fermentation, the fermentation broth was bead-beaten and extracted with an equal volume of ethyl acetate. The resulting organic phase was evaporated to dryness and reconstituted with methanol. Glabridin content was determined by ultra-performance liquid chromatography (UPLC) or liquid chromatography-mass spectrometry (LC-MS). Fermentation products of the engineered strain were quantitatively determined using an Agilent 1260 UPLC system. A Poroshell 120EC-C18 column with a particle size of 2.7 μm and a 3.0 × 100 mm diameter was used. The detection wavelength was 254 nm, and the column oven was set at 35°C. Mobile phase A consisted of 0.1% formic acid in water and mobile phase B consisted of acetonitrile. The gradient elution profile was as follows: 0 min, 20% B; 0–4.5 min, 20%–35% B; 4.5–6 min, 35%–50% B; 6–12 min, 50%–65% B; 12–18 min, 65%–85% B; 18–22.5 min, 85%–95% B; 22.5–24 min, 95%–20% B; and 24–30 min, 20% B. The flow rate was 0.2 mL / min, and the injection volume was 1 μL. External standard methods were used for qualitative and quantitative determination. The results showed that strain GLA2-2 produced 6.0 mg / L of glabridin.

[0148] Example 9 Enhancement and Verification of NADPH and Acetyl-CoA Supply in Glabridin Synthetic Strain

[0149] Genes ZWF1, MAE1, and ACC1 from Saccharomyces cerevisiae BY4741 and NHMGR from Silicibacter pomeroyi were amplified by PCR. OE-PCR and Gibson assembly were used to construct the following gene circuits: RTG3 -P ATP7 -ZWF1-TGAD1 -P SED1 -MAE1-T URC2 -P HSP12 -ACC1-T EGD2 -P GCY1 -NHMGR-T SUI2 -LEU2-YPRC3R. The promoter and terminator used were commonly used elements. The above gene circuit was transformed into strain GLA2-2 using lithium acetate transformation. The transformed bacteria were plated on SD-LEU2 solid medium and cultured at 30°C for 3 days. Positive clones were identified by colony PCR. The strain was named GLA2-3.

[0150] The strain was fermented in YPD medium at 30°C and 200 rpm for 5 days. After fermentation, the fermentation broth was bead-beaten and extracted with an equal volume of ethyl acetate. The resulting organic phase was evaporated to dryness and reconstituted with methanol. Glabridin content was determined by ultra-performance liquid chromatography (UPLC) or liquid chromatography-mass spectrometry (LC-MS). Fermentation products of the engineered strain were quantitatively determined using an Agilent 1260 UPLC system. A Poroshell 120EC-C18 column with a particle size of 2.7 μm and a 3.0 × 100 mm diameter was used. The detection wavelength was 254 nm, the column oven was at 35°C, and the mobile phase A was 0.1% formic acid in water. The mobile phase B was acetonitrile. The gradient elution profile was: 0 min, 20% B; 0–4.5 min, 20%–35% B; 4.5–6 min, 35%–50% B; 6–12 min, 50%–65% B; 12–18 min, 65%–85% B; 18–22.5 min, 85%–95% B; 22.5–24 min, 95%–20% B; and 24–30 min, 20% B. The flow rate was 0.2 mL / min, and the injection volume was 1 μL. External standard methods were used for qualitative and quantitative determination. The results showed that strain GLA2-3 produced 6.9 mg / L of glabridin.

[0151] Example 10 Induced Regulation of Enzymes in Glabridin Synthetic Strain to Improve Product Synthesis Efficiency

[0152] Due to the ubiquitous nature of DMT catalytic substrates, an inducible expression of GgDMT was designed. The following gene circuits were constructed using OE-PCR and Gibson assembly: ①T SUI2 -P GAL1 -GgDMT-T CPP3 -URA3-YPRC3R;②T SUI2 -P xyl -GgDMT-T CPP3 -URA3-YPRC3R. Among them P GAL1is the endogenous galactose-inducible promoter of Saccharomyces cerevisiae and is a conventional element; P xyl It is a synthetic xylose-inducible promoter (sequence see Chen Y, Zhang S, Young EM, Jones TS, Densmore D, Voigt CA. Genetic circuit design automation for yeast. Nat Microbiol. 2020, 5(11): 1349-1360).

[0153] The above gene circuit was transformed into strain GLA2-3 by lithium acetate transformation. The transformed bacteria were spread on SD-URA solid medium and cultured at 30°C for 3 days. Positive clones were obtained by colony PCR verification. The strains were named GLA2-4 (P GAL1 ) and GLA2-5(P xyl ).

[0154] The strain was fermented in YPD medium with a shaker at 30°C and 200 rpm. The medium was replaced at 96 h. The specific method was as follows: the culture was centrifuged at 5000 rpm for 3 min in a sterile 50 mL centrifuge tube, the supernatant was discarded, and YPG (10 g / L yeast powder, 20 g / L peptone, 20 g / L galactose) or YPX (10 g / L yeast powder, 20 g / L peptone, 20 g / L xylose) medium was added to the bacterial pellet, and the mixture was added to the original shake flask and fermentation was continued until the end of 5 days. The corresponding samples were named: GLA2-4 and GLA2-5.

[0155] After fermentation, the fermentation broth was bead-beaten and extracted with an equal volume of ethyl acetate. The resulting organic phase was evaporated to dryness and reconstituted with methanol. Glabridin content was determined by ultra-performance liquid chromatography (UPLC) or liquid chromatography-mass spectrometry (LC-MS). The fermentation products of the engineered strain were quantitatively determined using an Agilent 1260 UPLC system. A Poroshell 120EC-C18 column with a particle size of 2.7 μm and a 3.0 × 100 mm diameter was used. The detection wavelength was 254 nm, and the column oven was set at 35°C. Mobile phase A consisted of 0.1% formic acid in water and mobile phase B consisted of acetonitrile. The gradient elution profile was as follows: 0 min, 20% B; 0–4.5 min, 20%–35% B; 4.5–6 min, 35%–50% B; 6–12 min, 50%–65% B; 12–18 min, 65%–85% B; 18–22.5 min, 85%–95% B; 22.5–24 min, 95%–20% B; and 24–30 min, 20% B. The flow rate was 0.2 mL / min, and the injection volume was 1 μL. External standard analysis was used for qualitative and quantitative determination. The results showed that samples GLA2-4 and GLA2-5 produced 7.2 mg / L and 7.8 mg / L of glabridin, respectively.

[0156] The glabridin-producing strain GLA2-5 was subjected to fed-batch fermentation. A single colony from a plate was inoculated into a test tube containing 5 mL of YPD liquid medium and cultured overnight at 30°C, 200 rpm. A 10% transfer ratio was then made to a 250 mL Erlenmeyer flask containing 50 mL of YPD liquid medium and cultured at 30°C, 200 rpm for 24 hours to obtain a seed solution. This seed solution was inoculated into a 5 L fermentor containing 2.5 L of fermentation medium. The fermentation medium consisted of 40 g / L glucose, 10 g / L yeast extract, 10 g / L corn steep liquor, 5 g / L (NH4)2SO4, 8 g / L KH2PO4, 3 g / L MgSO4·7H2O, 12 mL / L vitamin solution, and 10 mL / L trace metal salt solution. The fermentation temperature was 30°C, the pH was controlled to be not less than 5.0 by adding ammonia water, a 50% glucose solution with a total volume of 1000 mL was added within 24 to 120 hours, and 200 mL of a 40% xylose solution was added after 120 hours. The feeding rate was controlled so that the ethanol concentration in the culture medium was not higher than 20 g / L. The stirring speed was controlled between 300 and 500 rpm and the ventilation volume was controlled between 0.5 and 1 vvm so that the relative dissolved oxygen after 24 hours was maintained at approximately 30%.

[0157] After 168 hours of fed-batch fermentation, the fermentation broth was bead-beaten and extracted with an equal volume of ethyl acetate. The resulting organic phase was evaporated to dryness and reconstituted with methanol. Glabridin content was determined by ultra-performance liquid chromatography (UPLC) or liquid chromatography-mass spectrometry (LC-MS). The fermentation products of the engineered strain were quantitatively determined using an Agilent 1260 UPLC system. A Poroshell 120EC-C18 column with a 2.7 μm particle size and a 3.0 × 100 mm diameter was used. The detection wavelength was 254 nm, the column oven was 35°C, and the mobile phase A was 0.1% formic acid in water. The mobile phase B was acetonitrile. The gradient elution profile was: 0 min, 20% B; 0–4.5 min, 20%–35% B; 4.5–6 min, 35%–50% B; 6–12 min, 50%–65% B; 12–18 min, 65%–85% B; 18–22.5 min, 85%–95% B; 22.5–24 min, 95%–20% B; and 24–30 min, 20% B. The flow rate was 0.2 mL / min, and the injection volume was 1 μL. External standard methods were used for qualitative and quantitative determination. The results showed that the concentration of glabridin in the fermentation broth reached 31.6 mg / L.

[0158] Example 11 Construction and Verification of a Yarrowia lipolytica Strain Synthesizing Glabridin

[0159] In order to illustrate that the synthesis method of glabridin is also applicable to other yeasts, this example selected Yarrowia lipolytica as the chassis cell to construct the glabridin synthesis route. Using OE-PCR and Gibson assembly methods, the following gene circuit was constructed on the pYL15 plasmid (GenBank accession: KU378202): PGI -GgPAL-T GPD -P ENO -GgC4H-T MDH -P TEF -SeTAL-T CYC -P TPI -Gg4CL-T HHF -P GPM -GgCHS-T ALD -P GPP -GgCHR-T LSC -P PDC -GgCHI-T DLD -P MDH -GuCPR2-T SGA -P CIT -GgIFS-T COX8 -P HHF -GgHID-T RPS31 -P TDH-GgOMT-T LYS -P COX4 -GgI2'HT DPM -P PCK -GgIFR-T ADE -P CIT -GgVR-T PLC -P TEF -GgPT1-T RPN -P PGK -GgPTR1-T TYC -P TDH -GgOC1-T TFA -P HSP26 -GgDMT1-T CPP The promoter and terminator used are elements commonly used in Yarrowia lipolytica.

[0160] The plasmid was transformed into Yarrowia lipolytica ATCC 201249 by lithium acetate transformation. The transformed bacteria were spread on SD-URA solid medium and cultured at 30°C for 3 days. Positive clones were obtained by colony PCR verification.

[0161] The strain was fermented in YPD medium at 30°C and 200 rpm for 5 days. After fermentation, the fermentation broth was bead-beaten and extracted with an equal volume of ethyl acetate. The resulting organic phase was evaporated to dryness and reconstituted with methanol. Glabridin content was determined by ultra-performance liquid chromatography (UPLC) or liquid chromatography-mass spectrometry (LC-MS). Fermentation products of the engineered strain were quantitatively determined using an Agilent 1260 UPLC system. A Poroshell 120EC-C18 column with a particle size of 2.7 μm and a 3.0 × 100 mm diameter was used. The detection wavelength was 254 nm, the column oven was at 35°C, and the mobile phase A was 0.1% formic acid in water. The mobile phase B was acetonitrile. The gradient elution profile was: 0 min, 20% B; 0–4.5 min, 20%–35% B; 4.5–6 min, 35%–50% B; 6–12 min, 50%–65% B; 12–18 min, 65%–85% B; 18–22.5 min, 85%–95% B; 22.5–24 min, 95%–20% B; and 24–30 min, 20% B. The flow rate was 0.2 mL / min, and the injection volume was 1 μL. External standard methods were used for qualitative and quantitative determination. The results showed that this Yarrowia lipolytica strain could produce 2 mg / L of glabridin.

[0162] sequence:

[0163] SEQ ID NO: 1

[0164] MEFSNDNSNSNDSSILNLCNGGGGTTHDHHHHDDPLNWGMAADAMRGSHLDEVKRMV

[0165] EEYRKPVVPLGGKGLTISQVAAVATHSTGVAVELAKETRAAVKASSDWVVDSVNKGTDSY

[0166] GVTTGFGATSHRRTKLGGALQNELIRFLNAGIFSNGTESCHTLPHTATRAAMLVRINTLLQ

[0167] GYSGIRFEIMEAIANFLNHNITPCLPLRGTITASGDLVPLSYVAGLLIGRPNSKIGPNGQVLS

[0168] AKEAFQLAGIEGGFFELQPKEGLALVNGTAVGSGLASLVLFEANLFAILSEVMSAIFAEVMQ

[0169] GKPEFTDHLTHKLKHHPGGQIEAAAIMEHILDGSSYVKSAQKLHEIDPLQKPKQDRYALRTS

[0170] PQWLGPQIEVIRHATKMIEREINSVNDNPLIDVSRNKALHGGNFQGTPIGVSMDNTRLAIAS

[0171] MGKLMFAQFSELVNDFYNNGLPSNLTASRNPSLDYGFKGAEIAMASYCSELQYLANPVTN

[0172] HVQSAEQHNQDVNSLGLISSRKTAEAVDILKLMSSTFLVALCQAIDLRHMEENLKSTVKNI

[0173] VSQVAKRVLTVGANGELHPSRFCEKDLLKVVDREYVFAYIDDPCSATYPLMQKLRHVLVD

[0174] HALQNGDKEANSSTSIFQKIGAFEEELKAILPKEVESARVENGNPPAVPNRIKECRSYPLY

[0175] KFVRENLGTSLLTGEKIKSPGEECDKVFSALCDGRFIDPLLDCLKEWNGAPLPIC*

[0176] SEQ ID NO:2

[0177] MDLLLLEKTLLGLFIAAITAIAISKLRGRRFKLPPGPIPVPIFGNWLQVGDDLNHRNLTDLA

[0178] KRLGDIFLLRMGQRNLVVVSSPELAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFTVY

[0179] GEHWRKMRRIMTVPFFTNKVVQQYRFGWESEAASVVDDVRRNPDAAAGGIVLRRRLQL

[0180] MMYNNMYRIMFDRRFESEEDPLFMKLKALNGERSRLAQSFEYNYGDFIPILRPFLKGYLTI

[0181] CKEVKERRLKLFKDYFVDERMKLESTKSTSNEGLKCAIDHILDAQKKGEINEDNVLYIVEN

[0182] INVAAIETTLWSIEWGIAELVNHPEIQKKVRDEIDRVLGPGHQVTEPDMQKLPYLQAVIKET

[0183] LRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKRPEEFRPERFLEE

[0184] ESHVEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSKLDTAEKGG

[0185] QFSLHILKHSTIVAKPRSF*

[0186] SEQ ID NO:3

[0187] MAKKEDIIFRSKLPDIYIPKHLPLHAYCFEKLSEFGSRPCLINAPTGDVYTYYDVDLAARKV

[0188] ASGLNRLGLGRGEVIMILLPNSPEFVFAFLGASYFGAVATAANPFFTATEIAKQATASNAKLV

[0189] ITQASYRDKVKGLDAATKVVLVDSPSPPAEGEYFSFSELMEADENESPEVKVEADEVVALP

[0190] YSSGTTGLPKGVMLTHRGLVTSIAQQVDGENPNLYFHQEDVILCVLPLFHIYSLNSVLLCG

[0191] LRAKAAILLMPKFEINALLGLVDKHRVTVAAVVPPIVLAIARSPDLHRYDLSSIRILKSGGAP

[0192] LGKELEDTVRAKFPNAILGQGYGMTEAGPVLTMSLAFAKEPMDVKAGACGTVVRNAEM

[0193] KIVDPKTGYSLPRNQSGEICIRGDQIMKGYLNDAEATERTIDKEGWLHTGDIGYIDDDDEL

[0194] FIVDRLKELIKYKGFQVAPAEIEALLLSHPKISDAAVVPMKDEAAGEVPVAFVVTSNGHTDI

[0195] TEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAAGVPN*

[0196] >SEQ ID NO:4

[0197] MVSVAEIRKAQRAEGPANILAIGTANPPNCVDQSTYPDFYFKITNSEHKTELKEKFQRMCD

[0198] KSMIKKRYMYLTEEILKENPNICAYMAPSLDARQDMVVVEVPRLGKEAAVKAIKEWGQP

[0199] KSKITHLIFCTTSGVDMPGADYQLTKLLGLRPYVKRYMMYQQGCFAGGTVLRLAKDLAE

[0200] NNKGARVLVVCSEITAVTFRGPTDTHLDSLVGQALFGDGAAAVIVGSDPVPEIEKPIFELVW

[0201] TAQTIAPDSEGAIDGHLREVGLTFHLLKDVPGIVSKNIDKALTEAFQPLGISDYNSIFWIAHP

[0202] GGPAILDQVEQKLALKPEKMKATRDVLSDYGNMSSACVLFILDEMRKKSAQNGLKTTGE

[0203] GLEWGVLFGFGPGLTIETVVLHSVAI*

[0204] >SEQ ID NO:5

[0205] MAAAPTVPVIVLPSSSGQRKMPVMGLGTAPEATSKVTTKDAVLEAIKQGYRHFDAAAAY

[0206] GVEKSVGEAIAEALKLGLLASRDEVFITSKLWVTDNHPETIVPALKKSLRTLQLEYLDLILI

[0207] HWPIATKPGEVKYPIDVSDIVEFDMKGVWGSLEECQRLGLTKAIGVSNFSIKKLEKLLSFAT

[0208] IPPAVNQVEVNLGWQQEKLRAFCKEKGIVITAFSPLRKGASRGSNLVMDNDVLKEIADAH

[0209] GKTIAQICLRWLYEQGLTFVVKSYDKERMNQNLQIFDWSLTEDDYKKISEIYQERLIKGPT

[0210] KPLLDDLWDEE*

[0211] >SEQ ID NO:6

[0212] MAGAAPVITAIQVENVEFPPLYTSPATGKSYFLGGAGERGLVIEGNFIKFTGIGVYLEDKAV

[0213] ASLAPKWKGKSFEELRDTLEFFRDIISGPFEKLIRGSKIRPLSGPEYSKKVIENSVAHMKSCG

[0214] TYGDAEAAAIEQFAQAFKNVNFPPGASVFYRQSPDGILGLSFSQDGSIPEKEAAVIENKAVS

[0215] GVVLETMIGEHAVSPDLKRCLAERLPVLLNAGTTNFKIGNGN*

[0216] >SEQ ID NO:7

[0217] MLVELAITLLVIALFIHLRPTPSAKSKSLRHLPNPPSPKPRLPFVGHLHLLDKPLLHNSLIDLS

[0218] KRYGPLYSLYFGSMPTVVVSTPELFKLFLQTHEASSFNTRFQTSAIRRLTYDNSVAMVPFGP

[0219] YWKFIRKLIMNDLNLATTVNKLRPLRSQEIRKVLRVMALSAESQVPNLNVTEELLKWTNSTI

[0220] SRMMLGEAEEIRDIARDVLKIFGEYSLTDFIWPLKKLKVGQYEKRIDDIFNRFDPVIERVIK

[0221] KRQEIRKKKKERNGEVEEGEQSVVFLDTLLDFAEDETMEIKITKEQIKGLVVDFFSAGTDS

[0222] TAVATEWALSELINNPRVLQKAREEVDAVVGKDRLVDEADVQNLPYIRSIVKETFRMHPPL

[0223] PLVKRKCVQECEIDGYAIPEGALILFNVWAVGRDPKYWDRPTEFRPERFLENVGEGDQAV

[0224] DLRGQHFQLLPFGSGRRMCPGVNLATAGMATLLASVIQCFDLSVVGPQGKILKGNDAKVS

[0225] MEESAGLTVPRAHNLVCVPVARSSAVPKLFSS*

[0226] >SEQ ID NO:8

[0227] MASTTPTASTDTKTIVAEIPTYIRVFSDGTVERPRQAPTVPPMLDDPKTGVSSKDITISHHPK

[0228] ISARLFLPKLSDRAEKVPILVFFHGGGFFFESAFSQLYHDHFNSFVSQTNVLAVSVEYRLAP

[0229] EHPLPACYHDCWDALQWVSSHSTNNSPNINNIAEPWLIEHGDFNRVFIGGDSAGGNIVHNI

[0230] AMRAGDEALPCDVNILGAIFAHPYFYGSSPIGSEPVEKREEELCYLVWKLVYPSAPGGIDN

[0231] PMVNPLGPGAPSLAGLGCSKIIVCVAGKDKIRDRGVWYYEAVKKSGWKGEIELFEEKEED

[0232] HVYHIFHPESENGKKLIKRLALFLHE*

[0233] >SEQ ID NO:9

[0234] MDFSTNGSEESELYHAQIHLYKHVYNFVSSMALKSAMELGIADVIHNHGKPITLPQLASAL

[0235] KLHPSKVGVLYRFLRLLTHNGFFAKTTVPSQNGKEGEEEETAYALTPPSKLLVKGKPTCLAS

[0236] IVRGALHPSSLDMWRSSEKWFNEDKELTLFESATGESFWDFLNKDSESGTLSMFQEAMAA

[0237] DSQMFKLALKECRHVFEGLESLVDVGGGTGGVTKLIHEEFPHLKCTVFDQPQVVGNLSGN

[0238] ENLKFVGGDMFKSIPPADAVLLKWVLHDWNDELSLKILKNSKEAISGKGKEGKVIIIDISID

[0239] EASGDRELTELQLDYDLVMLTMFNGKEREKKEWEKLISDAGFSSYKITPICGFKSLIEVFP*

[0240] >SEQ ID NO:10

[0241] MEILSLLSYSVFYVALFFICNLLFQARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKY

[0242] GHVISLWFGSRLVVVVSSLSVFQECFTKNDIVLADRPRFLSGKYIFYNYTTVGSSSYGEHW

[0243] RNLRRITSLDVLSTHRINSFSGVRRDETQRLIQKLAEESSDTFAEIELTSKFYDMTFNNIMR

[0244] MISGKRYYGDDCDMMDMEEAKQFRAMVSELQLSGANNKTDFMPVLRLVDFENLEKRL

[0245] KKISSKTDTFLRGLIQEHRNKKQHTNTMVDHLLSLQESQPEYYTDQIIKGLAGLMLLAGT

[0246] DSSAVTLEWALSCLLNHPEALKKARDELETHVGQDRLLEESDLTKLPYLKNIIYETLRLYTP

[0247] APLLLPHSSSDECIIGGFKVPRETIILINAWSIHRDPQIWSEATSFKPERFEKEGELDKLIAFGL

[0248] GRRACPGEALALRGISLTLGLLIQCFEWKLVGDKEIDMREESGFTLSRLIPLKAMCKARPV

[0249] ANKLVNTQAVSLGD*

[0250] >SEQ ID NO:11

[0251] MNMIEVGGYPGNPINTKQSNAQQHKPTASLTTYQNKKKKNTHPSFFNSPYFYFNFNIPMA

[0252] AENKILILGPTGAIGRHIVWASVKAGNPTFALVRKTNGPVNKPKLITAANPESKEQLLESYQ

[0253] NAGVTLLEGDINDHESLVKAIKQVDVVICATGRLLIDDQVKIIAAIKEAGNVKRFFPSEFGL

[0254] DVDRHDSVEPVREVFEDKARIRRVIEAEGVPYTYLCCHAFTGYFLRNLAQLDATVPPRDK

[0255] VVILGDGNVKGAYVTEADVGTYTIRAANDPRTLNKAVHIRLPANYLTANEVVSLWEKKIG

[0256] KTLEKTYVPEEKVLKDIQESSFPHNYLLALYHSQQIKGDAVYEIDPAKDVEAYDLYSDVKY

[0257] TTADEYLNQFV*

[0258] >SEQ ID NO:12

[0259] MAEGKGRVCVTGGTGFLGSWLIKRLLEDGYGVNTTIRSDPERKRDISFLTNLPGASERLKI

[0260] FNADLSDPESFGPAIEGCVGVFHTASPIDFAVSEPEEIVTKRTVDGTLGILKACKNSKTVKRV

[0261] VYTSSGAAVSWGGTEKDVLDESDWSDVDMLRSVKPYSWSYAVSKTMTEKAVLEFGEQH

[0262] GLDVVTLILPFIVGRFICPKIPDSVEKALVLVLGKREQIGVTRFHMVHVEDVARAYIFLLEHP

[0263] NPKGRYNCSPFIVPIEEVSELLSAKYPEYQIPTVEELKEIKGAKLPDLKSKKLVDAGFEFKY

[0264] TIEDMFDDAIQCCKEKGYL*

[0265] >SEQ ID NO:13

[0266] MASQHKKKTQIEYNVLRFQRPSLGHGYNFSGGGGSTYQECNRKYAVKAVYDQPKDFELE

[0267] ASNPKNILDSAKKFLAAFYYFSYPYTMIGITLCAFCSSALAVEKLSDISLPFFIGVLQAVIPQL

[0268] FIEIYLSGVNQLYDLEIDKINKPHLPMASGQFSFKTGVIMSAAFLALSFGFTWLTGSWPLIW

[0269] NLVVIASSWTAYSIDVPFMRWKRYPLVAAMCMIATWGLALPISFFHHMQTFVLQRPIGFPR

[0270] SLGFLVAFMTFYSMGIALSKDIPDVEGDKEHGINSFSVLLGQKRIFWICVSLFEMAFGVGLL

[0271] GGVTSPHFWTKIITGLGNVILGSILWYQAKSFDLTDKASTGSFYMYIWKLLYAALLLMAFV

[0272] R*

[0273] >SEQ ID NO:14

[0274] MDSMVVGSFPNASSITTGGNLWLGKNCAKNYYATSFYTPKASQHKRKIQKEYNFLRSRQP

[0275] SLKHLYKGIEGGSTYQECNRKYVVQTAPKPSFESNPRSIDQKYNLESVKTFLDAFYMFCTP

[0276] YAFMCTVLYIASMSLLAVEKLSDISPLFFIGILQAYVPYLFIHIYVTGLNQVCDVEIDKINKP

[0277] YLPLASGKISFTTGVIIVVSCLILVLYIFIPFYNDNFQDDFTNSVEGGLLFYYFVQGLWLRLIV

[0278] GSWPSTWALISLVMTWGAYSINVPLLRWKKNPVLAAMCNFLTFGVTFPLGYFFHMQTFVF

[0279] KRPSFFPRPLIFATTIMSFFSLVIALFKDLPDIEGDQAFGVKTFATRFGQKWVFWICISLLEMA

[0280] YGVAILMGATSSFLWSKIVTGLGHAILASFLLYRAKSIDLRSKASIASFYKLIWKLMCVECF

[0281] LMPLVR*

[0282] >SEQ ID NO:15

[0283] MSSLFGSNVFESISTYRSYAPKASQHKRKIQKEYNFLRSRQPSLKHIYKGIEGGSTQQECNR

[0284] KYVVKTVPKPSFESDPRSIDQKDILECIKNFLDALYMFTTPYSFFSSALYIISVSFIVVEKLSD

[0285] ISPLFFTGVLQAVVPNLFVHIYMTGLNQLCDVEIDKINKPYLPLVSGKISFATGVIVVASCLF

[0286] LSLWLGWIVGSWPSTCVVISIAMIWTAYSINVPLLRWKKYPVLAAMCIFLSLAVVNPIGYFL

[0287] HMQTFVFKRPASFSRPLIFVIAFKSFFSLAVALFKDVPDIEGDQTFGVQSFVARFGKKRVFWI

[0288] CISLIEMAYGFALLMGATSAFLWSKIVTVLGHAVLASFVLYRAKSIDLRSKTSITSFYMLIW

[0289] KLMYMEYFLMPLVR*

[0290] >SEQ ID NO:16

[0291] MGSVAVVSFPNASSITIGGNLWRGKNCVNNYYAASSYTPKASQHNRKIEKKNNFLSSQQLS

[0292] LKHLYKGIEGGSTYQECNGKNVVKIAPKPSFESDSRSIERKNIMESIKNFLDVFYMFCTPRS

[0293] FIAPAFGAISSCLLVVEKLSDISPLFFIGVLQAVIPYLFISVYVHGINQLGDVEIDKINKPYLPL

[0294] ASGKLSFTTGVIIVASCLILGLGLGWIVGSWPLIWSLILSSVICAAYSVNVPLLRWKRNPVL

[0295] AAMCTVVNLGFVLPIANFLHMQTFVFKRPAFFSRPLIFATTFMGLYSLVIALFKDIPDIEGDQ

[0296] AFGVQTFAACFGQKRIFWICISLLEMAYGVAMLMGATSSCLWSKIVTGLGHAVLALVVLY

[0297] RARSVDLTSKTSLSSFYMLIWKLVHVEYLLMPLVR*

[0298] >SEQ ID NO:17

[0299] MDSVIVGSFPNASSITTGGNLWQGENCAKNYYATSSYTPKASQHKRKIQKEYNFLRSRQPS

[0300] LKHLYKGIEGGSTYQECNGKYTMKTAPKPSFESDPRSIDQNNILESTKTFLDAFYMFCTPY

[0301] ALICSVLFIISMSLLAVEKLSDISPLFFTGVLQALVPHMFVVIYITGINQVCDVEIDKINKPYL

[0302] PLASGKISFTTSVIVVASSLITGLSLSWIVGSWPSTWALISLTVIWGAYSLNVPLLRWKKNPV

[0303] LAAMCQFLTTAVVNPIGYFLHMQTFVLKRPIFFSRQLIFAIAIMSCFSLAVSLFKDLPDIEGD

[0304] QAFGIQTFAARFGQKRVFWICISLLEMAYGVALLMGATSSCLWSKIVMGLGHAVLASVVL

[0305] YCAKSIDLRSKASITSFYMLIWKLMYVESFLMPLAR*

[0306] >SEQ ID NO:18

[0307] MISVHVGSFPNTYTFITGRNLWQGQNCTKNFYASSYTPKASQQKRKIKQEYNFLRSRQPSL

[0308] KHLYKGIGGGSSYQECNRKYVVKTAHKPSFESDPRSIDRKNILESIKNFLHAFYMFCTPYAS

[0309] IVEKLSDISPLFFTGVMQAMVPHIFINIFVAGINQLTDAEIDKINKPHLPLASGKMSYTTGVII

[0310] VASSLILGLWFGWIIGSWPCILTLISFAVIWSAYSVNVPLLRWKRNPVLAAMCCVVTLSVAIP

[0311] IGYFFHMQTFVFKRQAFFSRPLIFAVASLNLFSLVLALFKDLPDVEGDREFGIQTFAMRLGQ

[0312] KRVFWICIFLLEMAYGIALLMGATSPYLWSKIVTVLGHAILALVVLYHAKKSIDLRSKATAI

[0313] SFYKFIWKLLFMELFLMPFVR*

[0314] >SEQ ID NO:19

[0315] MAIPLLKIEKISLAERTVPLLYDLLEFLCWQKISSTEASFIKDMKTEMRNKLIRRLKKLLSRS

[0316] VCRTKPSSPNHQTHVDVREEYANAFRTESYIEFWTRVLAYSNKGEYTSCLSREFTTSARLPS

[0317] YRLFAEHLLDPDQPTITRVLAQIQLRPEIHSLLSDYFAHTANASMLCSHILKDINRVRVKYTS

[0318] LKTILQCVPTKQISSSMVMTHLTEFSNSLNPFATSSPAQCRVRASELQKRLESSRDKARAKL

[0319] HLIARLKRCSACLVVIVTTSLVVFVVTHGFALLVAVSGLASMNLVSKRKLAKVTAQLDAAA

[0320] KGTYILNKDLETTSRLVARLNDELEHMRTTIKFWLEKKDDGIFQADGEVVRLLKKNQCGF

[0321] SDQLDELEEHLYLCFMTINRARDLVLNQITNSA*

[0322] >SEQ ID NO:20

[0323] MRNHWALVGGNLWQGQNCTKNCYASSYTPKASQQKRKIKKEYNFLRSQQPSLKHLYKGI

[0324] EGGSSYQECNRKYVVKTTHEPSFESDPRSIDRKNILESIKNFLHAFYLFCTPYSVIGTALGIIS

[0325] VSLLAVEKLSDISPLFFTGVIQAVVPHMFINIFITGINQLSDAEIDKINKPHLPLASGKMSYTT

[0326] GVIIVASSLIMGLWLGWIIGSWPCILTLISFAVIWGAYSVNVPLLRWKRNPVLAAMCNVVSL

[0327] SVAFPIGYFFHMQTFVFKRSAFFSRPLVFAIAFLNLFSQVIALSKDLPDIEGDREFGIQTFAAR

[0328] LGQKRVFWICIFLLEMTYGVALLMGATSPYLWSKIVTVLGHAILALVVLYRANSSIDLRSK

[0329] ASATSFYKFIWKLLYMELFLMPFVR*

[0330] >SEQ ID NO:21

[0331] MDSTAASKILVIGGTGYMGRFLVEASAKAGHPTFALVRHSTVTNPDKSSIIHSFNTLGVNLL

[0332] LGDINDHQSLVKAIKQADVVISTVNHQYISDQYKIISAIKEAGNIKRFFPSEFGNDVDRTHG

[0333] VDGAKALFDIKAKFRRTIEAEGIPHTYVVANFLTQHFLPTRSRLLAIAAPLDKVVILGDGNT

[0334] KATFNTEEGVATFTIRAVDDPRTLNKYLIRPPANTLSYNDLVSLWEKKTGNTLERVYVPEE

[0335] QVLRLIQESSYPLNMALSICHAAYVKGDHTNYEIEPSFGVEALELYPDVKFTTVDDYLEQN

[0336] RDCTPFYLNQLIPITNGTKF*

[0337] >SEQ ID NO:22

[0338] MSLPHSFFLFSLTLISNHVITRTHGVFSEQPNIMLPTDHHHHHQGTEKLTHIHFYYHDIRNN

[0339] KDPTIVQIIDTPKNVPNGFGSTFVMDDAMTEGPELSSKHIGRAQGLFGLASLQDLGMFMLT

[0340] NFVFTEGNYAGSTLSMLGRNPISEQNREMPIVGGTGVFRFARGYAIANSVNSISTPEHFVVE

[0341] YNITVSHP*

[0342] >SEQ ID NO:23

[0343] MAETTTSKSKILFIGGTGYIGKFIVEASVNAGHPTFVLIRDSTLSNPAKSPIIDKFKSLAVNLV

[0344] FGDLYDHQSLVKAIKQVDVVISTVGHLQLGDQDKIISAIKESGNVKRFFPSEFGNDVDRTH

[0345] AVEPAKSAFATKAKIRRTIEAEGIPYTYVSNFFAGYFLPTLSQPGATAAPRDKVIILGDGNP

[0346] KAVANKEEDIATYTIKAVDDPRTLNKYLYIRPPANTLSFNELVSLWEKKIGKTLGRVYVPEEQ

[0347] LLKQIQESSPPINVILSIGHSVYVKGDHTNFEIEPAFGVEASALYPDVKYTTVDEYLNQFV*

[0348] >SEQ ID NO:24

[0349] MAAAETKILFIGGTGYIGKFIVEASIKAGHPTYLLVRETSLSDPAKSPIIHKFKASGVKILLG

[0350] DLYDHQSLVEAIKQVDVVISTVGHWQLPDQDKIVSAIKEAGNVKRFFPSEFGNDVDRTHA

[0351] VEPIKTTYQVKAKLRRAIEAEGIPHTIVSANFGAGYFLPSLSQPGATSPPRDKLVILGDGNPK

[0352] CVFNSEVDVGTYTIKAVDDPRTLNKIVYIRPQANTLSFNELVSLWEKKIGKTLERVHVPED

[0353] QVLQQIQKTSDVVLSICHSVYVKGDQTNFEIEPSFGVEASALYPDVKYTTVDEFLNQFV*

[0354] >SEQ ID NO:25

[0355] MDSTAASKILVIGGTGYMGRFLVEASAKAGHPTFALVRHSTVTNPDKSSIIHSFNTLGVNLL

[0356] LGDINDHQSLVKAIKQADVVISTVNHQYISDQYKIISAIKEAGNIKRFFPSEFGNDVVRTHG

[0357] VDGAKALFDIKAKFRRTIEAEGIPHTYVVANFLTQHFLPTRSRLLAIAAPLDKVVILGDGNT

[0358] KATFNTEEGVATFTIRAVDDPRTLNKYLIRPPANTLSYNDLVSLWEKKTGNTLERVYVPEE

[0359] QVLRLIQESSYPLNMALSICHAAYVKGDHTNYEIEPSFGVEALELYPDVKFTTVDDYLEQN

[0360] RDCTPFYLNQLIPITNGTKF*

[0361] >SEQ ID NO:26

[0362] MGKSKVLVVGGTGGYIGRRIVKASLAEGHETYVLQRAELGFQIEKLQMLLSFKRQGAHLV

[0363] QASFSDHNSLVDAVKKVDVVISAISGVHIRTHCISLQLKLIDAIKQAGNIKRFLPSEFGLDPA

[0364] RMGHALEPGRVTFDDKMAIRKAIEEANIPFTYISANLFAGYFAGSLSQMGSFVPPRDKVHL

[0365] FGDGKLKAVFLEDDDVATYTIKSIDDPRTNLKTLYLRPPENILSQAELIGIWEKLIGKELEKT

[0366] YITPEGFLTKLKGLDYKLQVGIGHFYHIFYEGCLTNFEIGEDGEEASKLYPEVNYTRMDEY

[0367] LKIYV*

[0368] >SEQ ID NO:27

[0369] MAEKSKILIIGGTGYIGKHIVEASAKAGHPTFALVRESTLNSNPAKANLLDKFCTLGVNLVPG

[0370] DLYDHENLVKVIKQVDVVISTVGHLQLADQVKIIAAIKEAGNVKRFFPSEFGNDVDRVHA

[0371] VEPAKSAFAIKVQIRRTVEAGIPYTYVSSNCFAGYFLPTLAQPGATAPPPPRDKVIILGDGN

[0372] PKAVFNKEDDIATYTIRAVDDPRTLNKILYLRPPHNIYSFNELVALWEKKIGKTLEKTYVPEE

[0373] KLLKDIQEAPLPINVVLAINHSVFVKGDHTNFEIESSFGAEASELYPDVKYTTVDEFLDQFV

[0374] *

[0375] >SEQ ID NO:28

[0376] PAKSTASATFFTSLILLFLLLSVVTATYYQSMSPTMLGFQEEKFTHLHFYFHDVVTGPKPS

[0377] MVIVAEPNGKAKNSLPFGTVVAMDDPLTVGPESDSKLVGKAQGIYTSISQEEMGLMMVM

[0378] TMAFSDGEFNGSTLSILARNMIMSEPVREMAIVGGTGAFRFARGYAQAKFYSVDFTKGDA

[0379] IVEYDIFVFHY*

[0380] >SEQ ID NO:29

[0381] MGLLSLNRSIQLSSLLFPIAVLLSLSWYSASAANSTNNTFLHCLVNHSDPSHPITSAIFTPNN

[0382] SSFPSVLQAYIRNLRFNTTTTRKPFLIITALHVSHIQAAIICGQNHNLQMKIRSGGHDYEGVS

[0383] YVAEVPFFILDMFNLRSIEIDMDTETAWVQSGATLGELYYRIAEKSKHHGFPAGVCPTVGV

[0384] GGHISGGGYGNMMRKYGLSVDNVIDAQIVDAKGRLLDRKSMGEDLFWAIRGGGGGSFG

[0385] VVLSYKIKLVKVPEKVIVFQVQRTLEQNATDIVYNWQHVAPNIDNDLFIRVILDVINNTSTQ

[0386] NGTKTIRANFIALFLGDSKRLVSLLNESFPQLGLKESDCIETSWLQSVLFWDNKDIATPVEIL

[0387] LNRQPQSLNYLKRKSDYVKEPISKEGLEGIWKKMIELVDAKLYFNPYGGRMAEIPSSATSF

[0388] PHRAGNLWKVQYQANWNEAGEEVADHYIGLTRELRKYMTPFVSKNPRGAFLNYKDLDL

[0389] GINHNGKNSYFEGRVYGVEYFKDNFDRLVKIKTKVDPGNFFRNEQSIPTLPYRKS*

[0390] >SEQ ID NO:30

[0391] MMPLSSYFAVVVIAFLFAVASSTADNHESFFQCLQSYSHNSTSISKVVYTQTNSSYSSILRFS

[0392] THNRRFTSNATPKPLVIVTPLQVSEIQATIICSQRHGMHIRTRSGGHDYEGLSYVSHVDPFV

[0393] VIDLINLREIQVDVENSTAWVEAGATIGELYSISQKSRTLGFPSGFCPTVGVGGQISGGGY

[0394] GSLLRKYGLAVDHVIDARIIDVKGRLLDREAMGEDLFWAIRGGGGASFGVITAWKIKLVPV

[0395] PPTVTVFTVSKTLEQNATKLIHKWQHVANKLDEDLFIRISLARVNSSQTRKLTIQATFESLFL

[0396] GGVDRLLPLMQEVFPELGLIREGCTEMSWVESVLYLYEFTSGQPPLEVLLNRTTDFMFMK

[0397] GKSDYVRDPIPDSGLEGLWPMFYEDEAQDAVMTLQPYGGKMDEIPESEIFPPHRAGNIYA

[0398] AMHWVIWREEGDEVAQRHINWIRRLYSYMEPFVSKSPRAAYVNERDLDIGVNNINGYTSY

[0399] EQASIWGLKYFKNNFNRLAKVKTMVDPLNFFRNEQSIPSLM*

[0400] >SEQ ID NO:31

[0401] MHNTIINKMTPLSSYFTVVAIAFLFSIASTADNHESFFQCLQNYSHNSTSISKVVYTQTNSS

[0402] YSSILRFSTHNRRFTSNATPKPPLVIVTPLQVSEIQATIICSQRHGMHIRTRSGGHDYEGLSYVS

[0403] HVDPFVVIDLINLREIQVDVENSTAWVEAGATIGELYYSISQKSRTLGFPAGVCPTVGVGGQ

[0404] ISGGGYGPLLRKYGLAADNVIDARIIDVKGRLLDSEAMGEDLFWAIRGGGGASFGVITAW

[0405] KIKLVPVPPTVTVFRVSKTLEQNATKLIHKWQHLLANKLDGDLIITIKLARVNSSQTGKLTV

[0406] QATFECVFLGGVDRLLPLMQEVFPELGLIREDCTEMSWVESVLYNYGFTSGQPLEVLLNRT

[0407] SQADVLFFKGKSDYVRDPIPDSGLEGLWPMFYEDEAQDALLTLIPYGGKMDEIPESKIPFP

[0408] HRAGNIYSIQHWLYWQEEGDEVAQRHINWIRRLYSYMEPFVSKSPRAAYVNERDLDIGVN

[0409] NNGYTSYEQASIWGLKYFKNNFNRLATVKTRVDPLNFFRNEQSIPSLM*

[0410] >SEQ ID NO:32

[0411] MAAKPFLPLLFLLLLSIVSASLAAPNTTESLYTTFLQCLTQHTDPSISNLVFAQTNSSFSTVLQ

[0412] NYVRNARFNTSSTPKPLIIVTPEQEPHVQGTVLCAKSINVQLKIRSGGHDYEEGISYISNEPFII

[0413] LDLFNFRNITVDIKNEVAMVQSGATLGEVYYRIWEKSKVHGFPAGVCPTVGVGGHFSGGG

[0414] YGNMLRKFGLSVDNVIDAQIVDVKGRILNRESMGEDLFWAIRGGGGASFGVILSYTVKLV

[0415] RVPETVTVFRVEKTLEQNATDLVVQWQQVAPTTDDRLFMRLLLQPVSSKVVKGTKTVRA

[0416] SVVALFLGVADEVVSLLGKEFPLLGLKKENCTEVSWIDSVLWWDDDESLKNGAKPETLLN

[0417] RNLNSASFLKRKSDYVQNAISRDGLEWIWKRMIELGKTGFVFNPYGGKMGEIASDATPFP

[0418] HRAGNLFKIQYSVNWDDPSQAMALNFTNQAKRLYSYMTPFVSKNPRSAFLNYRDLDIGV

[0419] NSFGKDSYEEGKVYGSKYFNDNFERLVKVKTAVDPENFFRNEQSIPVLPAGKA*

[0420] >SEQ ID NO:33

[0421] MAFTYSTKKLALLSIITIFISSFPEPSTSVDLESGFLQCFSSGLGNSNSTAELVLTKNSSSYASL

[0422] LQSSIRNLRFMGTSVPKPTLIVTPRDLFHIQTSIKCSVKQGLQIRVRSGGHDYEGLSYVSND

[0423] DVPFLIIDLTNLRSITIDIKDETAWVQSGATLGELYYAIAKKSNVHGFPAGSCPTVGVGGHFS

[0424] GGGFGTIFRKYGLAADNVIDAQMVDVNGKILKNRTLMGEDLFWAIRGGGGSSFGVVTAW

[0425] KVKLVHVPPKVTVFNIPKTLDQNASTLFHKWQIVADKLPGELFLHSVMGVSNANSISSSSG

[0426] GGRTVLVSFTGLYLGTVENLLPLMQNNFAELGLQHNNCTEMSWIQSVLFFAGYDPINDSLE

[0427] VLLQRNQTFGSFKAKSDYVMEPIPTSGLEGLWNMLLEENSSPTLILTPYGGRMSEISESETP

[0428] FPHRNGSIYGIQYLVYWDSSEETPKHIAWMRRVYSYMVLYVSKFPRAAYLNYRDLDIGVN

[0429] RGNTSYDYEEAKSWGLKYFKCNFERLAKVKAEVDPSNFFRHEQSIPLLF*

[0430] >SEQ ID NO:34

[0431] MVSPSSHLAILILLISVSFTASSASTIEESFVQCLSFYSDKAAPFYATIYTPNNASFTKILNSSA

[0432] QNLRYLVPSAPKPEFIFTPLTDSHVQVAVICSKKLGIHLRVRSGGHDYEGLSYVSEIETPFIIID

[0433] LAKLRDINVDIEDNTAWIQAGATIGEVYYRIYEKSSVHGFPAGLCTSLGVGGHITGGAYGS

[0434] MMRKYGLGADNVLDAQIVDANGKILDRKAMGEDLFWAIRGGGGGSFGILLWWKIKLVP

[0435] VPETVTVFTVTKSLEQDATRLVHRWQEVAPYIDEELFIRVIIQPATVGNTTKRTITTSYNALF

[0436] LGGADRLLQVMKESFPELGLTKKDCLETSWIKSVLYIAGFPNDTPPEVLLNGKSTFKNYFK

[0437] AKSDFVREPIPETGLQGLWQRLLEEDSPLMIWNPYGGMMSNFSESDIPFPHRNGTLYKIQY

[0438] LTLWQDGDKNASKHIDWTRKLYNYMTPYVSKFPREAYVNYRDLDLGMNKKNSTSYIQAT

[0439] AWGNMYFKDNFNRLVKIKTKVDPENVFRHEQSIPPLPVSTMQLKDKKCKNWE*

[0440] >SEQ ID NO:35

[0441] MQALRSFLASFLVLLSISLTTSTPIEETFNHCLTLHSQPPNQFPSTIYTSRNDSYTSILNSTAQN

[0442] LRYLLPSVPKPDFIFTPFHDSQVQAAVICAKKLGIHMRVRSGGHDYEGLSYVSLIEKPFMIL

[0443] DLVKLRAVNVDLPHNTAWIQAGATIGEVYYRISEKSAVHGFPAGLCTTLGIGGHITGGAYG

[0444] SMMRKYGLGADNVLDARIVDANGRILDRAAMGEDLFWAIRGGGGGSFGVILWWKIKLVP

[0445] VPKTVTVFTVTKSLEQGASKLLQRWQKVAPNIDEDLFIRVIIQPGNGSVPGQRTVTTSYNA

[0446] LFLGGAGRLLQVMKHSFPELGLTRKDCLETSWIKSVLYIAGYPNGTPPEVLLQGKPTSKAY

[0447] FKAKSDFVRQVIPEASLNAIWKVFLQDDGPLMIWNPYGGMMGRIAESATPFPHRKGTLYK

[0448] IQYVTGWIDGEKSMAKHMNWMRKFYYCMAPYASKYPRETYVNYRDLDIGMNQKNCTS

[0449] FSQAHSWGYRYFKGNFNRLVKVKTKVDPSNFFRHEQSIPPLSTGKKG*

[0450] >SEQ ID NO:36

[0451] MVSPISLPWPILVLLLLSSSFSLPLPVSASIQESFIQCLNLNSDRTFPFHTTIYTPNDASFITILD

[0452] SSAQNLRCLLPSAPKPEFIFTPSRDSHVQAAVICSKKLGIHLRVRSGGHDYEGVSYVSEIETP

[0453] FMVVDLVKLRGINVDIKTNTAWVQAGATIGEVYYRIYEKSSVLGFPAGLCTSLGVGGHITG

[0454] GAYGTMMRKYGLGADNVLDAQIVDANGKILNRAAMGEDLFWAIRGGGGGSLGILLWW

[0455] KIKLVPVPPTVTVFTVTKSLEQGATKLLHRWQEVAPYIDENLFIRVIIQPASGGGNKTQRTIT

[0456] TSYNAVFLGEARRLLQVMKTSFPELGLTMKDCQETSWIKSVLYIAGFPSGTPPEVLLKGKP

[0457] TFKNSFKAKSDFVREPIPETGIEGLWQRLLIEDSPLMIWNPYGGRMSQFSESDTPFPHRNGT

[0458] LYKIQYLSLWQDGDKNAAKHIDWIRKLYNYMTPYVSNFPRQAYVNYRDLDLGMNSKNST

[0459] SYIQASAWGYRYFKDNFNRLVKIKTRVDPENVFRHEQSIPPLPI*

[0460] >SEQ ID NO:37

[0461] MLVDTGLGLISELQAKLGWAVLLQIVPITIVAYNLLWFIYASFFSSLRKIPGPFLARISRVWE

[0462] MKKTATGNIHEIMMDLHRRHGAIVRIGPRRYDFDTMEALKIIYRIGNALPKADYYKPFGLP

[0463] SFPNLFDEQNPARHSAIKKQVASLYTMTALLSYEEGVDGQTAILKEQLQRFCDQKQVIDLP

[0464] RFLQYYAFDVIGVITVGKSMGMMESNSDTNGACSALDGMWHYASMMAYIPNMHAWWL

[0465] RLSSLLPIEVPIKGLTEYVERRIIQYRLKAAEFGDDAALKGENNFLAKLLLMEKKGTVTPVE

[0466] TQQAVGLNIGAGSDTTANALSTILYYLYTNPRTLHTLREELERYVKDGPISFQQSQSMPYLQ

[0467] AVIKEALRLHPGVGTQLTRVVPKGGLVIEGQFFPEGTEVGVNGWALYHNKAIFGNDASIFR

[0468] PERWLEANENINIGGSFAFGAGSRSCIGKNISILEMSKAIPQIVRNFDIEINHGDMTWKNEC

[0469] WWFVKPEYKAMIKPRRCCLSRDESLV*

[0470] >SEQ ID NO:38

[0471] MAGNVEKLLPKSIEEMSMDGYEPPSEYVVKGNNSFGSKDSSTLIPIPIIDVSLLSSEGELEKL

[0472] RSALSSAGCFQAIGHGMSTSYLDKVREVAKQFFALPVEEKQRYARAVNESEGYGNDRIVSE

[0473] KQVLDWSYRLTLRVFPKEQRRLSLWPENPTDFSETLEEFSAKVKSMMDSLLRSMARSLNL

[0474] EEGSFLDQFGKQSSLQARFNFYPRCSRPDFVLGVKPHTDRSGITVLLQDKEVEGLQVLVD

[0475] DKWVNVPTIPDALVVNLGDQMQIMSNGIFKSPMHRVVTNTEKLRMSVAMFNEPEPENEIG

[0476] PVKDLINETRPRVYRNVKNYGEINYRCYQEGKIALETVQIADNCDQK*

[0477] >SEQ ID NO:39

[0478] MDVSSILVPSVQELAKEALTRVPERYVLNPDQDPLPLSNNSAPLPDQVPVIDLSKLLSQDDL

[0479] KGPAELEKLNSACKDWGFFQLVNHGISTSLVENMKTGVKTLFELSMDEKKQLWQREGDL

[0480] EGFGQAFIVSEEQKLEWADAFFMITLPPHLRKPHLFNQIPQSFRENLEIYSAELENLAIKIIEL

[0481] MANALAIDPKEMTEVFKVGIQTIRVNYYPPCPQPERVIGLKSHSDSGGLTILLQVNDIEGLQ

[0482] IRKDGLWIPVQPLPNAFIINIGDMLEIMTNGIYRSIEHRATVNSEKERISLATFYGPNMQAIL

[0483] APAPSLVTPERPEQFRRISVADYFKGYFDQELSGKSYLDEVKIPKGE*

[0484] >SEQ ID NO:40

[0485] MEHFYMSLLLLFVTLVSLSLFFLIFYHNKHNMNNNNNLPPGKMGYPVIGESLEFLSMGWK

[0486] GHPEKFIFDRMVRYSSELIKTSILGVPTVIFCGPACNKFLFSNENKLVTAWWPDSVNKIFPTT

[0487] SNSKEESKKMRKLLPQFLKPEALQRYVGIMDTLAQRHFASLWEEKTHVTVYPLAKRYTF

[0488] MLACRLFMSVEDENHVAKFREPFHLLASGIISVPIDLPWTPFNRGIKASNFIRKELLKIIRQR

[0489] KVDLAQGVASPTQDILSHMLLTCDDENGEFMTELNIADKILGLLIGGHDTASAACTFIVKY

[0490] LAELPHIYDRVYQEQMEIANSKSPGELLNWDDINKMRYSWNVASEVMRVAPPLQGGFRE

[0491] AINDFVFNGFSPKGWKLYWSANSTHKNPEYFPAPEKFDPTRFEGNGPAAYTFVPFGGGPR

[0492] MCPGKEYARLEILVFMHNLVKRFKWEMLIPEEKIVVDPLPMPANDLPIRLYPHNT*

[0493] >SEQ ID NO:41

[0494] MDATTLTVPSVQEIAKGSLTTVPERYVRPPHERPTTLFATTPLPDQVPVIDLSKLLSQDDLK

[0495] GPAELEKLHSACKDWGFFQLINHGVSISLLENVKKGAQEFFNLPIEEKKKKFGQKEGDVE

[0496] GYGQAFVVSEEQKLEWADLFYLLTLPPHMRKPHLFPNLPLPFRDDLEAYTAELKNLAIQVL

[0497] DLMANALKMDAKEMRELFGEGVQSMRMNYYPPCPQPELVMGNLPHSDGGGLTILLLQAN

[0498] EVEGLQIKKDGLWIPVKPLPNAFIINLGDMLEMITNGIYRSIEHRATVNLEKERLSIATFYNP

[0499] GVEITVRPAPSLVTPKTPAVFKTIGVLEFYRGYFARELQGKSYLDTMRIQSEDEKSS*

[0500] >SEQ ID NO:42

[0501] MANGEISNYTELNVRQGEPTRVKPAEETEGLYFLSNLDQNIAVPVRTVYCFKSASRGNED

[0502] AARVIKDSLSKILVPYYPMAGKLMISSEGKLIVDNNTGEGAVFVEAEADCDIQDIGDLTKP

[0503] DPDTLGKLVYNTPGARSILEMPLMTVQVTKFKCGGFTLGLNMIHCMKDGLCAMEFVNA

[0504] WSETARGLDTKTPPFLDRTILKARDPPKIEFQHHEFDEIEDISNTENLYEQEKMFYRSFCFDP

[0505] EKLEQLKKKATEDGVLEKCSTFEALSAFIWRARTEALKMHPDQKTKLLFAVDGRSRFIPPV

[0506] PEGYFGNAIVLTNSLCNASELLGNPLSFTVGLVRKAIDMVNDSYMRSAIDYFELTRARPSLT

[0507] ATLLITTWTRLSFHTTDFGWGEPVCSGPVTLPEKQVILFLSQGQERKSINVLLGLPASAMES

[0508] FEALVMQV*

[0509] >SEQ ID NO:43

[0510] MDSQTKKLGTSLLVPSVHELAKQSLAEVPERYVRPNQDPTLVPNTISLPQVPAIDLNKLLSE

[0511] DGTELEMLDHACKEWGFFQLINHGVNPSLVENVKTGVQEFFSLPMEEKKKFWQKPGELE

[0512] GYGQNFVVSEEQKLEWADLFYIITLPSYTRNHHLFSSIPKSFRNDLESYCLEMEKLCITIIEL

[0513] MAKALKIKPNELLELFEDVSQSMRMNCYPPCPQPEHVMGLNPHSDAGALTILLQANEIEG

[0514] LQIRKDGMWIPIKPLSNAFVINVGDILEILTNGIYRSIEHRATINLGQERISIATFHRPQMHRV

[0515] VGPMSTLITSERPSLYKRIGVADYYKGYFSRELQGKSYIDVVRIQKG*

[0516] >SEQ ID NO:44

[0517] MEELNNPSGRYLLVPSVQELAKEKITNVPPKYIQPEHENLGIISEDECVLEIPVIDMQRLISL

[0518] EFGSSSESELAKLHLACKEWGFFHSALALQSFIYLFLINHGISSSFLENVKLEIQDFFNLPML

[0519] EKKKFWQSPQHMEGFGQAFVLSEDQKLDWADMFYMLTLPTHLRMPHLFPQLPLPFRETL

[0520] ELYSQEIKILAMSIITHIEKALKMQEMEIRELFEDGIQMMRMNYYPPCPQPEKVIGLTNHSD

[0521] RVGLTILLQVNEIEGLQIKKNGMWVRVKPLPNAFIVNIGDILEIMTNGIYPSIEHRATVSSEK

[0522] ERLSIATFHSPRHDGVVGPAASLITEQTQAQFKRIGVDEYFKGVFARKLDGKSYIYVMRIK

[0523] HHD*

[0524] >SEQ ID NO:45

[0525] MDATTLTVPSVQEIAKGSLTTVPERYVRPPHERPTTLFATTPLPDQVPVIDLSKLLSQDDLE

[0526] GPAELEKLHSACKDWGFFQLINHGVSISLLENVKKGAQEFFNLPIEEKKKKFGQKEDVEG

[0527] YGQAFVVSEEQKLEWADLFFLLTLPPHMRKPHLFPNLPLPFRDDLEAYTAELKNLAIQVLD

[0528] LMANALKVDAKEMRELFGEGVQSMRMNYYPPCPQPELVMGLNPHSDGGGLTILLQANE

[0529] VEGLQIKKDGLWIPVKPLPNAFIVNLGDMLEMITNGIYRSIEHRATVNLEKERLSIATFYNP

[0530] GVEITVRPAPSLVTPKTPAVFKTIGVLEFYRGYLARELRGKSYLDTMRIQSQDEKSS*

[0531] >SEQ ID NO:46

[0532] MDATTLTVPSVQEIAKGSLTTVPERYVRPPHERPTTLFATTPLPDQVPVIDLGKLLSQDDLK

[0533] GPAELEKLHSACKDWGFFQLINHGVSISLLENVKKGAQEFFNLPIEEKKKKFGQKEGDVE

[0534] GYGQAFVVSEEQKLEWADLFYLLTLPPHMRKPHLFPNLPLPFRDDLEAYTAELKNLAIQVL

[0535] DLMANALKMDAKEMRELFGEGVQSMRMNYYPPCPQPELVMGNLPHSDGGGLTILLLQAN

[0536] EVEGLQIKKYGLWIPVKPLPNAFIINLGDMLEMITNGIYRSIEHRATVNLEKERLSIATFYNP

[0537] GVEITVRPAPSLVTPKTPAVFKTIGVLEFYRGYFARELRGKSYLDTMRIQSEDEKSS*

[0538] >SEQ ID NO:47

[0539] MDATSLTVPSVQEIAKGSLTTVPERYVRPPHQRPTTLFATTPLPDQVPVIDLSKLLSQDDLK

[0540] GPAELEKLHSACKDWGFFQLINHGVSISLLENVKKGAQEFFNLPIEEKKKKFGQKEGDVE

[0541] GYGQAFVVSEEQKLEWADLFFLLTLPPHMRKPHLFPNLPLPFRDDLEAYTAELKNLAIQVL

[0542] DLMANALKVDAKEMRELFGEGVQSMRMNYYPPCPQPELVMGLNPHSDGGGLTILLQAN

[0543] EVEGLQIKKDGLWIPVKPLPNAFIINLGDMLEMITNGIYRSIEHRATVNLEKERLSIATFYNP

[0544] GVEITVRPAPSLVTPKTPAVFKTIGVLEFYRGYLARELQGKSYLDTMRIQSEDEKSS*

[0545] >SEQ ID NO:48

[0546] MDVSSILVPSVQELAKEALTRVPERYVLNPDQDPLPLSNNSAPLPDQVPVIDLSKLLSQDDL

[0547] KGPAELEKLHSACKDWGFFQLVNHGISTSLVENMKTGVKTLFELSMDEKKQQWQREGDL

[0548] EGFGQAFIVSEEQKLEWADAFFMITLPPHLRKPHLFNQIPQSFRENLEIYSAELENLAIKIIEL

[0549] MANALAIDPKEMTEVFKVGTQTIRVNYYPPCPQPERVIGLKSHSDSGGLTILLQVNDIEGL

[0550] QIRKDGLWIPVQPLPNAFIINIGDMLEIMTNGIYRSIEHRATVNSEKERISLATFYGPNMQAI

[0551] LAPAPSLVTPERPEQFRRISVADYFKGYFDQELSGKSYLDEVKIPKGE*

[0552] >SEQ ID NO:49

[0553] MDATTLTVPSVQEIAKGSLTTVPERYVRPPHERPTTLFATTLPDQVPVIDLSKLLSQDDLEG

[0554] PAELEKLHSACKDWGFFQLINHGVSISLLENVKKGAQEFFNLPIEEKKKKFGQKEGDVEG

[0555] YGQAFVVSEEQKLEWADLFYLLTLPPHMRKPHLFPNLPLPFRDDLEAYTAELKNLAIQVLD

[0556] LMANALKMDAKEMRELFGEGVQSMRMNYYPPCPQPELVMGNLPHSDGGGLTILLLQANE

[0557] VEGLQIKKDGLWIPVKPLPNAFIINLGDMLEMITNGIYRSIEHRATVNLEKERLSIATFYNPG

[0558] VEITVRPAPSLVTPKTPAVFKTIGVLEFYRGYFARELQGKSYLDTMRIQSEDEKSS*

[0559] >SEQ ID NO:50

[0560] MDATTLTVPSVQEIAKGSLTTVPERYVRPPHERPTTLFATTPLPDQVPVIDLSKLLSQDDLK

[0561] GPAELEKLHSACKDWGFFQLINHGVSISLLENVKKGAQEFFNLPIEEKKKKFGQKEGDVE

[0562] GYGQAFVVSEEQKLEWADLFFLLTLPAHMRKPHLFPNLPLPFRDDLEAYTAELKNLAIQVL

[0563] DLMANALKMDAKEMRELFGEGVQSMRMNYYPPCPQPELVMGNLPHSDGGGLTILLLQAN

[0564] EVEGLQIKKDGLWIPVKPLPNAFIINLGDMLEMITNGIYRSIEHRATVNLEKERLSIATFYNP

[0565] GVEITVRPAPSLVTPKTPAVFKTIGVLEFYRGYLARELRGKSYLDTMRIQSEDEKSS*

[0566] >SEQ ID NO:51

[0567] MDVSSILVPSVQELAKEALTRVPERYVLNPDQDPLPLSNNSAPLPDQVPVIDLSKLLSQDDL

[0568] KGPDELEKLHSACKDWGFFQLVNHGISTSLVENMKTGVKTLFELSMDEKKQLWQREGDL

[0569] EGFGQAFIVSEEQKLEWADAFFMITLPPHLRIPHLFNQIPQSFRENLEIYSAELENLAIKIIEL

[0570] MANALAIDKPEMTEVFKVGIQTIRVNYYPPCPQPERVIGLKSHSDSGGLTILLQVNDIEGLQ

[0571] IRKDGLWIPVQPLPNAFIINIGDMLEIMTNGIYRSIEHRATVNSEKERISLATFYAPSLVTPERP

[0572] EQFRRISVADYFKGYDFQELSGKSYLDEVKIPKGE*

[0573] >SEQ ID NO:52

[0574] MTSNSDLVRTIESVLGVSLGDSVSDSLVLIATTSVAVIIGLLVFLWKKSSDRSREVRPVIVPKS

[0575] LVKDEDDDVDVASGKTKVTVFFGTQTGTAEGFAKALADEIKARYEKAYVKVVDLDDYA

[0576] MDDDQYEEKLKKETLAFFMLATYGDGEPTDNAARFYKWFTEGKEERGTWLQQLTHGVF

[0577] GLGNKQYEHFNKIGKVVDEDLSEQGAKRLVPLGLGDDDQSIEDDFSAWKESLWPELDQL

[0578] LRDEDDVNTVSTPYTAAIPEYRVVIHDSTVTPSYDNQFSAANGGAVFDIHHPCRVNVAVKR

[0579] ELHKPQSDRSCIHLEFDISGTGITYETGDHVGVYAENCDETVEEAGKLLGQNLDLLFSLHT

[0580] DNEDGTSLGGSLLPPFPGPCTLRTALARYADLLNPPRKAALVVLAAHASEPSEAERLKFLSS

[0581] PQGKDEYSKWVVGSQRSLLEVMAEFPSAKPPLGVFFAAIAPRLQPRYYSISSSPRFASQRV

[0582] HVTCALVYGPTPTGRIHKGVCSTWMKNAIPLEESRDCGWAPIFIRPSNFKLPADHSIPIIMV

[0583] GPGTGLAPFRGFLQERFALKEDGVQLGPSLLFFGCRNRQMDFIYEDELKNFVEQGSLSELI

[0584] VAFSREGPEKEYVQHKMMDKAAYLWSLISQGGYLYVCGDAKGMARDVHRILHTIVQQQ

[0585] ENVESSKAEAIVKKLQMDGRYLRDVW*

[0586] >SEQ ID NO:53

[0587] ATGGAAACAATATCAGCAGCTATCACAAAAAACAGTGCCAATGAATCATTCTGTTTGAT

[0588] TCATGCTAAGAATAATAACATGAAAGTGAATGAAGCTGATCCTTTGAATTGGGGTGTGG

[0589] CAGCTGAAGCAATGAAAGGCAGTCACTTGGATGAAGTGAAACGTATGGTGGAGGAGT

[0590] ACCGGAAGCCGGTGGTCCGTCTTGGTGGCGAAACACTGACCATTTCTCAGGTGGCTGC

[0591] CATTGCTGCACATGACCATGGTGTGCAGGTGGAGCTGTCAGAATCTGCTAGGGCTGGT

[0592] GTTGAGGCCAGCAGTGATTGGGTGATGGAGAGTATGAACAAAGGAACAGACAGTTAC

[0593] GGTGTCACCACCGGATTCGGCGCCACCTCACACCGCCGTACCAAACAAGGTGGTGCTT

[0594] TGCAGAAAGAACTCATCAGATTTTTGAATGCTGGAATATTTGGAAACGGAACTGAGTC

[0595] AAGCCACACACTTCCACACACAGCAACAAGAGCTGCCATGCTAGTGAGAATCAACAC

[0596] ACTCCTCCAAGGTTATTCAGGAATCAGATTTGAAATCTTGGAAGCCATCACCAATCTCC

[0597] TTAACAACAACGTCACTCCATGTTTACCACTTCGCGGTACAATCACAGCTTCAGGAGAT

[0598] TTGGTCCCTCTATCTTACATTGCTGGTTTACTAACTGGAAGACCAAATTCCAAAGCTCAT

[0599] GGACCATCTGGAGAAATACTTAATGCAAAAGAAGCTTTTGCATTGGCTGGAATCAATGC

[0600] TGAATTCTTCGAATTACAACCAAAAGAAGGCCTTGCACTTGTTAACGGAACAGCTGTT

[0601] GGTTCCGGTTTAGCTTCAATTGTTCTCTTCGAGGCTAACATTTTGGCTGTGTTGTCTGAA

[0602] GTTCTATCAGCTATTTTTGCTGAAGTTATGCAAGGGAAACCTGAGTTTACCGATCATTTG

[0603] ACACACAAGTTGAAACACCACCCTGGTCAAATTGAGGCTGCTGCTATTATGGAACACA

[0604] TTTTGGATGGAAGTGCTTATGTCAAAGCAGATAAAAAGTTGCATGAGATGGATCCATTG

[0605] CAGAAACCAAAACAAGATAGATATGCGCTTAGAACTTCACCACAATGGCTTGGTCCTTT

[0606] GGTTGAAGTGATTAGATTCTCTACCAAGTCAATTGAGAGAGAGATCAACTCTGTCAACG

[0607] ACAACCCCTTGATTGATGTTTCAAGAAACAAAGCTTTGCATGGTGGAAACTTTCAAGG

[0608] AACGCCTATTGGAGTATCCATGGATAATACACGTTTGGCTCTCGCATCAATTGGCAAACT

[0609] TATGTTTGCTCAATTCTCTGAGCTTGTCAATGATTTTTACAACAATGGATTGCCTTCAAA

[0610] TCTTTCTGCGAGTAGAAATCCTAGTTTGGATTATGGTTTCAAGGGAGCTGAAATTGCCA

[0611] TGGCTTCCTATTGTTCTGAGTTGCAATATCTTGCAAATCCGGTTACAACCCACGTCCAAA

[0612] GTGCCGAGCAGCACAACCAAGATGTGAACTCTTTGGGTTTGATTTCTTCGAGAAAAAC

[0613] ATATGAAGCCATTGAGATCCTTCAACTCATGTCTTCCACATTCTTGATTGCTCTTTGCCA

[0614] AGCAATTGATTTAAGACATTTGGAGGAGAACTTGAAAAACTCAGTCAAGAACACCGTA

[0615] AGTCAAGTCGCCAAGACCCTTACCATAGGCGTGAATGGAGAACTTCATCCTTCAA

[0616] GATTTTGTGAAAGACTTATTGAAAGTGGTTGATAGAGAGCATGTATTTGCTTATATTG

[0617] ATGATCCTTGTAGTGCTACATACCCATTGAGTCAAAAACTCAGGCAAGTGTTGGTAGAT

[0618] CATGCATTAGTTAATGGAGAGAGTGAGAAGAATTTGAACACTTCAATCTTTCAAAAGAT

[0619] TGCAACTTTTGAGGAAGAGTTGAAGAGCCTCTTGCCAAAAAGAGGTTGAAAGTGCAAG

[0620] GACCGCATATGAGAGTGGAAACCCAACAATTCCAAACAAGATCAATGGATGCAGATCT

[0621] TATCCACTTTACAAGTTTTGTTAGAGAGGAGTTGGGAACTGGTTTACTAACTGGAGAAA

[0622] ATGTCATTTCACCCGGTGAAGTGTGTGACAAATTATTCACAGCTATGGTCAGGGAAAA

[0623] ATCATTGATCCTCTTCTTGAATGCTTGGGAGAGTGGAACGGTGCTCCTCTTCCAATTTGT

[0624] TAA

[0625] >SEQ ID NO:54

[0626] ATGGAGTTTCTCTAACGACAATTCCAATTCTAACGACTCTTCTATTCTAAACTTGTGTAAT

[0627] GGCGGTGGTGGTACAACTCATGATCATCATCACCATGATGATCCATTGAATTGGGGTATG

[0628] GCTGCTGATGCTATGAGAGGTTCTCATTTAGATGAAGTCAAGAGAATGGTTGAAGAGTA

[0629] CAGAAAACCAGTCGTTCCATTGGGTGGTAAGGGTTTGACAATCTCTCAAGTTGCTGCT

[0630] GTTGCAACTCATTCCACTGGTGTTGCAGTTGAATTGGCAAAGGAAACTAGAGCAGCTG

[0631] TTAAAGCCTCTTCTGATTGGGTTGTTGACTCTGTCAATAAAGGTACAGATAGCTACGGT

[0632] GTTACCACTGGTTTCGGTGCTACATCTCATAGAAGAACCAAATTGGGTGGTGCTTTGCA

[0633] GAACGAACTTATCAGATTCTTGAACGCTGGTATCTTCTCCAATGGTACTGAATCATGCCA

[0634] TACTTTGCCACATACTGCTACAAGAGCTGCAATGTTGGTCAGAATCAATACTTTATTGCA

[0635] GGGTTATAGTGGTATCAGATTTGAGATCATGGAAGCAATCGCAAACTTCTTGAACCATA

[0636] ATATTACTCCATGTTTACCTTTAAGAGGTACTATCACTGCATCAGGCGATTTGGTTCCATT

[0637] GTCATACGTCGCTGGTTTATTAATCGGTAGACCAAACTCCAAATCTATTGGACCTAATGG

[0638] TCAAGTGTTGTCTGCTAAAGAAGCATTCCAGTTAGCAGGTATTGAAGGTGGCTTCTTCG

[0639] AACTTCAACCTAAAGAAGGTCTAGCCTTAGTTAACGGTACTGCTGTTGGTTCTGGTTTA

[0640] GCTTCTTTTGGTTCTATTTGAGGCAAACTTGTTCGCTATTCTGTCTGAAGTCATGTCTGCT

[0641] ATCTTTGCTGAAGTTATGCAAGGTAAACCTGAATTTACTGACCATTTGACTCACAAATT

[0642] GAAGCATCATCCAGGTCAAATCGAAGCTGCTGCTATTATGGAACATATCTTGGATGGCT

[0643] CATCATACGTTAAGTCTGCTCAGAAGTTGCACGAAATTGATCCACTACAGAAACCAAA

[0644] GCAAGACAGATATGCCTTGAGAACATCACCACAATGGTTGGGTCCACAAATAGAGGTT

[0645] ATCAGACATGCTACTAAGATGATCGAAAGAGAAATTAATTCCGTCAATGACAATCCATT

[0646] GATCGATGTTTCTCGTAATAAAGCTCTACATGGTGGTAACTTTCAAGGTACTCCAATTGG

[0647] TGTTAGTATGGATAACACCAGATTGGCTATTGCCTCTATGGGTAAATTGATGTTTGCTCA

[0648] ATTCTCTGAATTGGTTAATGATTTCTACAATAATGGTTTGCCATCTAACTTGACTGCTTCA

[0649] AGAAATCCATCTTTGGACTATGGCTTTAAAGGTGCAGAAATTGCAATGGCATCTTACTG

[0650] TTCTGAATTGCAATACTTGGCAAATCCAGTTACAAATCATGTTCAATCAGCTGAACAGC

[0651] ATAATCAAGACGTTAACTCATTGGGTTTGATTTCATCAAGAAAGACTGCTGAAGCTGTT

[0652] GATATTCTGAAATTGATGTCATCCACCTTCTTGGTCGCTTTGTGTCAAGCTATCGATTTA

[0653] AGACACATGGAAGAGAATTTGAAGTCTACTGTTAAGAACATCGTTTCACAAGTTGCTA

[0654] AGAGAGTTCTAACTGTTGGTGCTAATGGTGAATTGCATCCATCTCGTTTCTGTGAGAAAA

[0655] GATTTGTTGAAGGTTGTAGACAGGAATACGTGTTTGCTTATATCGACGATCCATGTTCA

[0656] GCTACCTATCCATTGATGCAGAAGTTGAGACATGTTCTTGTCGATCATGCTTTACAAAAT

[0657] GGTGATAAAGAAGCCAATAGTTCTACAAGCATCTTTCAAAGATTGGTGCATTTGAAGA

[0658] AGAACTGAAGGCAATCTTGCCTAAAGAAGTTGAATCTGCTAGAGTTGAAGTCGAGAAT

[0659] GGTAATCCAGCTGTTCCAAATAGAATCAAAGAGTGTAGATCTTATCCATTGTACAAATTC

[0660] GTGAGAGAGAATCTGGGTACATCTCTTCTGACTGGTGAGAAGATCAAGTCTCCAGGTG

[0661] AAGAATGCGATAAAGTGTTTAGTGCCTTATGTGATGGCAGATTTATCGATCATTACTAG

[0662] ATTGTCTTAAAGAGTGGAATGGTGCACCACTACCAATTTGTTAA

[0663] SEQ ID NO:55

[0664] ATGGACCTCCTCTTGCTGGAGAAGTCTTTAATCGCCGTCTTCGTGGGCGGTGATTCTCGC

[0665] CACGGTGATTTCAAAGCTCCGCGGCAAGAAATTGAAGCTACCTCCAGGTCCTATACCA

[0666] ATTCCGATCTTCGGAAACTGGCTTCAAGTCGGAGATGATCTCAACCACCCGTAATCTCGT

[0667] CGATTACGCTAAGAAATTCGGCGATCTCTTCCTCCTCCGTATGGGTCAGCGAAACCTAG

[0668] TCGTCGTCTCCTCACCGGATCTAACAAAGGAAGTGCTCCTCACTCAAGGCGTTGAGTT

[0669] TGGATCCAGAACGAGAAACGTCGTGTTCGACATTTTCACCCGGGAAAGGTCAAGATATG

[0670] GTGTTCACTGTTTACGGCGAGCATTGGAGGAAGATGAGAAGAATCATGACGGTTCCTT

[0671] TCTTCACCAACAAAGTTGTTCAACAGAATCGTGAAGGTTGGGAGTTTGAAGCAGCTAG

[0672] TGTTGTTGAAGATGTTAAGAAGAATCCAGATTCTGCTACGAAAGGAATCGTGTTGAGG

[0673] AAACGTTTGCAATTGATGATGTATAACAATATGTTCCGTATCATGTTCGATAGAAGATTT

[0674] GAGAGTGAGGATGATCCTCTTTTCCTTAGGCTTAAGGCTTTGAATGGTGAGAGAAGTCG

[0675] ATTAGCTCAGAGCTTTGAGTATAACTATGGAGATTTCATTCCTATCCTTAGACCATTCCTC

[0676] AGAGGCTATTTGAAGATTTGTCAAGATGTGAAAGATCGAAGAATCGCTCTTTTCAAGA

[0677] AGTACTTTGTTGATGAGAGGAAGCAAATTGCGAGTTCTAAGCCTACAGGTAGTGAAGG

[0678] ATTGAAATGTGCCATTGATCACATCCTTGAAGCTGAGCAGAAGGGAGAAATCAACGAG

[0679] GACAATGTTCTTTACATCGTCGAGAACATCAATGTCGCCGCGATTGAGACAACATTGTG

[0680] GTCTATCGAGTGGGGAATTGCAGAGCTAGTGAACCATCCTGAAATCCAGAGTAAGCTA

[0681] AGGAACGAACTCGACACAGTTCTTGGACCGGGTGTGCAAGTCACCGAGCCTGATCTTC

[0682] ACAAACTTCCATACCTTCAAGCTGTGGTTAAGGAGACTCTTCGTCTGAGAATGGCGATT

[0683] CCTCTCCTCGTGCCTCACATGAACCTCCATGATGCGAAGCTCGCTGGCTACGATATCCC

[0684] AGCAGAAAGCAAAATCCTTGTTAATGCTTGGTGGCTAGCAAACAACCCCAACAGCTGG

[0685] AAGAAGCCTGAAGAGTTTAGACCAGAGAGGTTCTTTGAAGAAGAATCGCACGTGGAA

[0686] GCTAACGGTAATGACTTCAGGTATGTGCCATTTGGTGTTGGACGTCGAAGCTGTCCCGG

[0687] GATTATATTGGCATTGCCTATTTTGGGGATCACCATTGGTAGGATGGTCCAGAACTTCGA

[0688] GCTTCTCTCCTCCTCCAGGACAGTCTAAAGTGGATACTAGTGAGAAAGGTGGACAATTC

[0689] AGCTTGCACATCCTTAACCACTCCATAATCGTTATGAAACCAAGGAACTGTTAA

[0690] SEQ ID NO:56

[0691] ATGGACTTGTTGCTGTTGGAAAAGACTTTGTTGGGTTTGTTTATCGCTGCTATCACTGCT

[0692] ATTGCTATCTCTAAGTTGAGAGGTAGAAGATTCAAATTGCCTCCAGGTCCAATCCCAGT

[0693] TCCAATATTCGGTAACTGGTTGCAAGTTGGTGACGATTTGAACCATAGAAATCTGACTG

[0694] ATTTGGCTAAGAGATTGGGTGACATCTTTCTGCTAAGAATGGGTCAGAGAAACTTGGTC

[0695] GTTGTTTCTTTCTCCAGAATTGGCTAAAGAAGTCTTGCATACTCAAGGTGTTGAATTTGG

[0696] TTCAAGAACTAGAAATGTCGTCTTTGACATCTTCACTGGTAAAGGTCAAGACATGGTAT

[0697] TCACTGTCTATGGTGAACATTGGAGAAAGATGAGAAGAATCATGACTGTTCCATTCTTT

[0698] ACCAACAAAGTCGTTCAACAGTACAGATTCGGTTGGGAATCTGAAGCTGCTTCTGTTG

[0699] TTGATGATGTCAGAAGAAATCCAGATGCAGCTGCTGGTGGTATTGTCTTGAGAAGAAG

[0700] ATTGCAACTGATGATGTACAATAACATGTACAGAATCATGTTTGACAGAAGATTCGAAA

[0701] GTGAAGAAGATCCATTGTTCATGAAGTTGAAGGCTTTGAATGGTGAAAGATCCAGATT

[0702] GGCTCAATCTTTCGAATACAACTACGGTGACTTCATTCCAATCTTGCGTCCATTTCTGAA

[0703] GGGTTACTTGACTATCTGCAAAGAAGTCAAAGAGAGAAGATTGAAGTTGTTCAAAGAC

[0704] TACTTCGTTGATGAAAGAATGAAGCTGGAATCTACCAAGTCTACTTCCAATGAAGGTTT

[0705] GAAGTGTGCTATTGACCATATCTTGGATGCTCAGAAGAAGGGTGAAATTAATGAAGATA

[0706] ACGTCTTGTACATCGTCGAGAATATCAACGTTGCTGCTATTGAGACTACTTTGTGGTCTA

[0707] TCGAATGGGGTATTGCTGAATTGGTCAATCATCCAGAAATTCAGAAGAAAGTTAGAGAT

[0708] GAAATCGATAGAGTTCTAGGTCCAGGTCATCAAGTCACTGAACCAGATATGCAGAAAC

[0709] TGCCATACTTGCAAGCTGTCATCAAAGAGACTTTGAGATTGAGAATGGCTATTCCATG

[0710] CTAGTACCTCACATGAACTTGCATGATGCCAAATTGGGTGGTTACGATATTCCAGCTGA

[0711] ATCTAAGATCTTGGTCAATGCTTGGTGGTTGGCAAATAATCCAGCTAATTGGAAACGTC

[0712] CAGAAGAGTTCAGACCAGAAAGATTCTTGGAAGAAGAATCTCATGTTGAAGCTAATGG

[0713] TAACGACTTTAGATACTTGCCATTTGGTGTCGGTAGAAGATCTTGTCCAGGTATCATCTT

[0714] GGCTTTGCCAATCTTGGGTATCACTTTGGGTAGATTGGTTCAGAACTTCGAATTGTTGCC

[0715] ACCACCAGGTCAATCCAAGTTGGATACTGCTGAGAAAGGTGGTCAATTCTCTTTGCAC

[0716] ATTCTGAAGCACTCTACCATTGTTGCTAAACCAAGATCATTCTAA

[0717] >SEQ ID NO:57

[0718] ATGACTCAGGTCGTTGAACGACAGGCAGACCGACTGTCCTCTCGGGAGTACCTTGCTC

[0719] GAGTTGTGCGATCTGCCGGCTGGGACGCCGGACTGACATCCTGCACTGACGAGGAGAT

[0720] CGTTAGAATGGGTGCCTCAGCTCGAACCATTGAGGAGTATCTGAAATCCGACAAACCA

[0721] ATATACGGTCTTACCCAGGGATTTGGACCCCTGGTCCTTTTCGATGCTGACTCCGAGTTA

[0722] GAGCAGGGAGGCTCCCTGATCTCGCACCTTGGCACCGGACAGGGTGCCCCCCTGGCTC

[0723] CTGAGGTTTCTCGGCTCATCCTGTGGCTGAGAATCCAAAACATGCGAAAGGGATACTC

[0724] CGCTGTGTCCCCAGTCTTCTGGCAGAAGCTCGCTGACTTATGGAACAAGGGATTCACG

[0725] CCTGCTATCCCTCGACATGGTACTGTCTCTGCTTCTGGCGACCTCCAACCCCTCGCCCAT

[0726] GCTGCCTTAGCTTTCACCGGTGTGGGTGAGGCTTGGACTCGAGACGCTGACGGTAGAT

[0727] GGAGCACGGTCCCTGCAGTGGATGCTCTGGCAGCTCTGGGCGCCGAGCCTTTCGACTG

[0728] GCCTGTGCGAGAGGCCCTCGCTTTCGTGAACGGTACCGGAGCCTCGTTAGCCGTGGCC

[0729] GTCCTGAACCATCGTTCGGCCCTCCGACTCGTTCGAGCCTGCGCTGTCCTTTCAGCCAG

[0730] ATTAGCCACGCTCCTCGGCGCTAATCCCGAACACTACGACGTCGGCCACGGTGTCGCC

[0731] CGGGGACAGGTGGGACAGCTGACGGCAGCAGAGTGGATCCGACAAGGCCTGCCACGA

[0732] GGCATGGTCCGTGACGGATCCCGACCACTGCAGGAGCCTTACTCTCTGCGATGCGCCC

[0733] CACAGGTTTTAGGAGCCGTCCTCGACCAGCTTGATGGCGCTGGTGACGTGCTGGCCCG

[0734] AGAGGTGGACGGCTGTCAGGATAACCCCATCACCTACGAGGGTGAGCTTTTACATGGC

[0735] GGTAACTTCCACGCTATGCCCGTCGGCTTTGCATCGGACCAGATCGGACTTGCTATGCA

[0736] TATGGCCGCTTATCTGGCCGAGCGACAGCTTGGTCTTCTTGTGTCCCCTGTCACAAACG

[0737] GAGACCTCCCCCCTATGCTTACCCCCCGAGCTGGACGGGGTGCCGGACTGGCTGGTGT

[0738] CCAGATCTCCGCTACCTCATTCGTCTCGCGTATCCGACAACTCGTCTTTCCAGCCTCCCT

[0739] CACCACTCTTCCAACTAATGGCTGGAACCAGGACCATGTTCCTATGGCACTGAACGGC

[0740] GCTAACTCTGTCTTTGAGGCTCTGGAGCTGGGTTGGCTGACCGTTGGCTCCCTCGCTGT

[0741] GGGCGTGGCTCAGCTCGCAGCCATGACAGGTCACGCTGCCGAAGGTGTGTGGGCTGA

[0742] GTTAGCTGGTATCTGTCCCCCACTGGACGCCGACCGACCTTTAGGCGCTGAGGTCCGA

[0743] GCAGCCAGAGACCTCCTGTCCGCTCACGCAGACCAGCTTCTGGTCGACGAGGCCGAC

[0744] GGTAAGGATTTCGGATAG

[0745] >SEQ ID NO:58

[0746] ATGGGTGACTGTGTTGCTCCAAAGGAAGACTTGATCTTCAGATCTAAGTTGCCAGACAT

[0747] CTACATCCCAAAGCACTTGCCATTGCACACTTACTGTTTCGAAAACATCTCTAAGGTTG

[0748] GTGACAAGTCTTGTTTGATCAACGGTGCTACTGGTGAAACTTTCACTTACTCTCAAGTT

[0749] GAATTGTTGTCTAGAAAGGTTGCTTCTGGTTTGAACAAGTTGGGTATCCAACAAGGTG

[0750] ACACTATCATGTGTTGTTGTGCCAAACTCTCCAGAATACTTCTTCGCTTTCTTGGGTGCTT

[0751] CTTACAGAGGTGCTATCTCTACTATGGCTAACCCATTCTTCACTTCTGCTGAAGTTATCA

[0752] AGCAATTGAAGGCTTCTTTGGCTAAGTTGATCATCACTCAAGCTTGTTACGTTGACAAG

[0753] GTTAAGGACTACGCTGCTGAAAAGAACATCCAAATCATCTGTATCGACGACGCTCCAC

[0754] AAGACTGTTTGCACTTCTCTAAGTTGATGGAAGCTGACGAATCTGAAATGCCAGAAGT

[0755] TGTTATCGACTCTGACGACGTGTTGCTTTGCCATACTCTTCTGGTACTACTGGTTTGCC

[0756] AAAGGGTGTTATGTTGACTCACAAGGGTTTGGTTACTTCTGTTGCTCAACAAGTTGACG

[0757] GTGACAACCCAAACTTGTACATGCACTCTGAAGACGTTATGATCTGTATCTTGCCATTGT

[0758] TCCACATCTACTCTTTGAACGCTGTTTTGTGTTGTGGTTTGAGAGCTGGTGTTACTATCT

[0759] TGATCATGCAAAAGTTCGACATCGTTCCATTCTTGGAATTGATCCAAAAGTACAAGGTT

[0760] ACTATCGGTCCATTCGTTCCACCAATCGTTTTGGCTATCGCTAAGTCTCCAGTTGTTGAC

[0761] AAGTACGACTTGTCTTCTGTTAGAACTGTTATGTCTGGTGCTGCTCCATTGGGTAAGGA

[0762] ATTGGAAGACGCTGTTAGAGCTAAGTTCCCAAACGCTAAGTTGGGTCAAGGTTACGGT

[0763] ATGACTGAAGCTGGTCCAGTTTTGGCTATGTGTTTGGCTTTCGCTAAGGAACCATACGA

[0764] AATCAAGTCTGGTGCTTGTGGTACTGTTGTTAGAAACGCTGAAATGAAGATCGTTGACC

[0765] CAGAAACTAACGCTTCTTTGCCAAGAAACCAAAGAGGTGAAATCTGTATCAGAGGTGA

[0766] CCAAATCATGAAGGGTTACTTGAACGACCCAGAATCTACTAGAACTACTATCGACGAAG

[0767] AAGGTTGGTTGCACACTGGTGACATCGGTTTCATCGACGACGACGACGAATTGTTCATC

[0768] GTTGACAGATTGAAGGAAATCATCAAGTACAAGGGTTTCCAAGTTGCTCCAGCTGAAT

[0769] TGGAAGCTTTGTTGTTGACTCACCCAACTATCTCTGACGCTGCTGTTGTTCCAATGATC

[0770] GACGAAAAGGCTGGTGAAGTTCCAGTTGCTTTCGTTGTTAGAACTAACGGTTTCACTA

[0771] CTACTGAAGAAGAAATCAAGCAATTCGTTTCTAAGCAAGTTGTTTTCTACAAGAGAATC

[0772] TTCAGAGTTTTCTTCGTTGACGCTATCCCAAAGTCTCCATCTGGTAAGATCTTGAGAAA

[0773] GGACTTGAGAGCTAAGATCGCTTCTGGTGACTTGCCAAAGTAA

[0774] >SEQ ID NO:59

[0775] ATGGCTAAGAAAGAAGATATAATCTTTAGATCAAAGTTGCCAGACATTTACATACCTAA

[0776] ACATTTACCATTACATGCTTACTGTTTCGAGAAGTTGTCTGAATTTGGTTCCAGACCATG

[0777] TTTGATCAACGCTCCAACAGGTGATGTGTACACTTATTACGACGTTGATTTGGCTGCCA

[0778] GAAAGGTCGCTTCTGGTCTTAACAGATTGGGTTTGGGTAGAGGTGAAGTCATTATGATT

[0779] CTGTTGCCTAATTCCCCTGAGTTCGTATTTGCTTTCTTGGGTGCTTCATACTTTGGTGCT

[0780] GTTGCTACAGCTGCTAATCCATTCTTTACTGCTACAGAAATCGCTAAGCAAGCAACAGC

[0781] TTCTAATGCTAAGTTGGTCATTACTCAAGCTAGTTACAGAGATAAGGTTAAGGGATTAG

[0782] ATGCTGCTACTAAGGTTGTGTTGGTTGATTCACCTTCTCCACCAGCTGAAGGTGAGTAC

[0783] TTCTCATTCTCCGAGTTAATGGAAGCTGATGAGAATGAATCACCAGAAGTTAAGGTTGA

[0784] AGCCGATGAAGTTGTTGCTTTGCCATACTCTTCCGGTACTACAGGTTTACCTAAAGGTG

[0785] TCATGTTGACACATAGAGGTTTAGTTACAAGTATTGCTCAACAAGTTGATGGTGAGAAT

[0786] CCAAATTTGTACTTTCATCAAGAAGATGTCATCTTGTGTGTATTGCCTTTATTCCACATCT

[0787] ATTCTTTGAACTCTGTCTTGTTATGTGGTTTAAGAGCTAAGGCTGCTATATTGCTTATGCC

[0788] TAAGTTCGAGATCAACGCATTGCTTGGTTTAGTTGATAAACATAGAGTTACAGTTGCTG

[0789] CTGTTGTTCCACCAATCGTTCTAGCTATTGCTAGAAGTCCAGATCTTCATAGATACGACT

[0790] TGTCTAGCATCAGAATCTTGAAGTCTGGTGGTGCACCTCTTGGTAAAGAGTTGGAAGAT

[0791] ACTGTCAGAGCTAAGTTTCCAAATGCTATCTTGGGTCAAGGTTATGGTATGACAGAAGC

[0792] TGGTCCAGTTCTAACAATGAGTCTAGCCTTTGCCAAAGAACCAATGGATGTCAAAGCA

[0793] GGTGCATGTGGTACTGTCGTTAGAAACGCAGAAATGAAGATCGTTGATCCAAAGACTG

[0794] GTTACTCATTACCTAGAAACCAAAGTGGTGAAATTTGTATCAGAGGCGATCAAATCATG

[0795] AAGGTTATCTGAATGATGCTGAAGCTACAGAAAGAACCATCGATAAAGAGGGTTGGT

[0796] TGCATACTGGTGACATTGGTTACATTGATGACGATGATGAATTGTTTATTGTTGATAGATT

[0797] GAAAGAATTGATCAAGTATAAGGGCTTTCAAGTCGCTCCAGCAGAAATTGAAGCATTG

[0798] TTGTTGTCTCATCCAAAGATCTCTGATGCAGCAGTTGTTCCAATGAAAGATGAAGCTGC

[0799] TGGTGAAGTTCCAGTAGCTTTCGTTGTCACTTCCAATGGTCATACTGACATTACTGAAG

[0800] ATGAAATTAAGCAATTCATCTCAAAGCAAGTTGTGTTCTACAAGAGAATTAACAGAGTG

[0801] TTCTTCATCGACGCTATCCCTAAGTCACCATCTGGTAAGATCTTGAGAAAGGATTTGAG

[0802] AGCTAAATTGGCCGCAGGTGTTCCAAACTAA

[0803] >SEQ ID NO:60

[0804] ATGGTTACTGTTGAAGAATACAGAAAGGCTCAAAGAGCTGAAGGTCCAGCTACTGTTA

[0805] TGGCTATCGGTACTGCTACTCCAACTAACTGTGTTGACCAATCTACTTACCCAGACTACT

[0806] ACTTCAGAATCACTAACTCTGAACACAAGACTGACTTGAAGGAAAAGTTCAAGAGAAT

[0807] GTGTGAAAAGTCTATGATCAAGAAGAGATACATGCACTTGACTGAAGAAATCTTGAAG

[0808] GAAAACCCATCTATGTGTGAATACATGGCTCCATCTTTGGACGCTAGACAAGACATCGT

[0809] TGTTGTTGAAGTTCCAAAGTTGGGTAAGGAAGCTGCTCAAAAGGCTATCAAGGAATGG

[0810] GGTCAACCAAAGTCTAAGATCACTCACTTGGTTTTCTGTACTACTTCTGGTGTTGACAT

[0811] GCCAGGTTGTGACTACCAATTGACTAAGTTGTTGGGTTTGAGACCATCTGTTAAGAGAT

[0812] TGATGATGTACCAACAAGGTTGTTTCGCTGGTGGTACTGTTTTGAGATTGGCTAAGGAC

[0813] TTGGCTGAAAACAACAAGGGTGCTAGAGTTTTGGTTGTTTGTTCTGAAATCACTGCTGT

[0814] TACTTTCAGAGGTCCAAACGACACTCACTTGGACTCTTTGGTTGGTCAAGCTTTGTTCG

[0815] GTGACGGTGCTGGTGCTATCATCATCGGTTCTGACCCAATCCCAGGTGTTGAAAGACCA

[0816] TTGTTCGAATTGGTTTCTGCTGCTCAAACTTTGTTGCCAGACTCTCACGGTGCTATCGA

[0817] CGGTCACTTGAGAGAAGTTGGTTTGACTTTCCACTTGTTGAAGGACGTTCCAGGGTTTG

[0818] ATCTCTAAGAACATCGAAAAGTCTTTGGAAGAAGCTTTCAGACCATTGTCTATCTCTGA

[0819] CTGGAACTCTTTGTTCTGGATCGCTCACCCAGGTGGTCCAGCTATCTTGGACCAAGTTG

[0820] AAATCAAGTTGGGTTTGAAGCCAGAAAAGTTGAAGGCTACTAGAAACGTTTTGTCTAA

[0821] CTACGGTAACATGTCTTCTGCTTGTGTTTTGTTCATCTTGGACGAAATGAGAAAGGCTT

[0822] CTGCTAAGGAAGGTTTGGGTACTACTGGTGAAGGTTTGGAATGGGGTGTTTTGTTCGGT

[0823] TTCGGTCCAGGTTTGACTGTTGAAACTGTTGTTTTGCACTCTGTTGCTACTTAA

[0824] >SEQ ID NO:61

[0825] ATGGTCAGTGTTGCTGAAAATTAGAAAGGCTCAAAGAGCTGAAGGTCCAGCTAATATTTT

[0826] AGCTATTGGTACTGCTAACCCCCCAAATTGTGTTGATCAATCTACATATCCCGACTTTTA

[0827] CTTCAAGATCACTAACTCTGAGCACAAAACTGAATTGAAGGAAAAAGTTCCAGAGGATG

[0828] TGTGATAAATCTATGATCAAGAAGAGGTACATGTACTTGACAGAAGAAATCTTGAAGGA

[0829] GAACCCAAATATCTGTGCTTATATGGCTCCATCTTTGGATGCTAGACAAGATATGGTTGT

[0830] TGTTGAAGTTCCAAGATTGGGTAAAGAAGCTGCTGTTAAAGCTATTAAGGAGTGGGGT

[0831] CAACCAAAATCTAAAATTACACACCTGATCTTCTGCACTACTTCTGGTGTTGATATGCCA

[0832] GGTGCTGATTATCAATTAACAAAGTTGCTGGGTCTGAGGCCTTATGTTAAAAGATATATG

[0833] ATGTACCAGCAGGGTTGTTTTGCTGGTGGTACAGTTTTGAGATTGGCTAAAGATTTGGC

[0834] TGAAAACAACAAGGGTGCTAGAGTTTTGGTTGTTTGTTCTGAAATCACCGCTGTTACAT

[0835] TTCGTGGTCCAACAGATACTCATTTGGATTCTTTGGTTGGTCAAGCTTTGTTTGGTGAC

[0836] GGTGCTGCTGCTGTTATTGTTGGTTCTGATCCAGTTCCAGAAATTGAAAAACCAATCTT

[0837] CGAGTTGGTCTGGACTGCTCAAACTATTGCTCCAGATTCTGAAGGTGCTATTGATGGTC

[0838] ATTTGAGAGAAGTTGGTTTGACATTTCACTTGTTGAAGGATGTTCCAGGTATTGTTTCTA

[0839] AGAACATCGATAAGGCTCTGACAGAAGCTTTTCAACCATTAGGTATTTCCGATTACAAC

[0840] TCTATCTTCTGGATCGCTCATCCAGGTGGTCCAGCTATTTTAGATCAAGTTGAACAAAA

[0841] GCTGGCCTTAAAACCAGAAAAAATGAAGGCTACAAGGGATGTTTTGTCTGATTATGGTA

[0842] ACATGTCCTCTGCTTGTGTTTTATTCATTCTGGATGAGATGAGGAAGAAGTCTGCTCAA

[0843] AATGGTTTGAAAACCACTGGTGAAGGTTTGGAATGGGGTGTTTTGTTTGGTTTTGGTCC

[0844] AGGTTTGACTATTGAAACTGTTGTTTTGCACTCCGTTGCTATTTAA

[0845] >SEQ ID NO:62

[0846] ATGGCTGCTGTCCAACTGTACCAGTTATTGTTCTGCCAAGTTCTTCTGGTCAAAGAAA

[0847] GATGCCAGTTATGGGTTTGGGTACTGCTCCAGAAGCTACTTCCAAAGTTACAACTAAAG

[0848] ATGCTGTATTAGAAGCTATCAAGCAAGGTTACAGACACTTTGATGCTGCTGCTGCTTATG

[0849] GTGTTGAGAAGTCTGTTGGTGAAGCTATTGCAGAAGCATTGAAGTTAGGTCTATTAGCT

[0850] TCCAGAGAGAAGTGTTCATTACCTCCAAATTGTGGGTGACTGATAACCATCCAGAAAC

[0851] AATCGTTCCAGCTTTGAAGAAGTCTCTGAGAACTTTGCAACTTGAATACTTGGATCTGA

[0852] TCTTGATCCACTGGCCAATCGCTACCAAACCAGGTGAAGTTAAGTATCCAATTGATGTT

[0853] TCTGACATCGTTGAGTTCGATATGAAAGGTGTTTGGGGTTCTTTGGAAGAATGTCAGAG

[0854] ATTGGGTTTAACTAAGGCTATTGGTGTTTCAAACTTCTCCATCAAGAAGTTGGAGAAGT

[0855] TGTTGTCATTCGCTACTATTCCACCTGCTGTTAATCAAGTTGAAGTGAACTTGGGTTGGC

[0856] AACAAGAGAAATTGAAGGGCTTTCTGCAAAGAGAAGGGTATTGTTATTACCGCATTCTCT

[0857] CCATTGAGAAAGGGTGCTTCCAGAGGTTCTAACTTGGTCATGGATAACGATGTTCTAAA

[0858] GGAAATTGCTGATGCTCATGGTAAGACTATTGCACAGATTTGCTTGAGATGGTTGTATG

[0859] AACAAGGTTTGACTTTCGTTGTCAAGTCTTACGACAAAGAGAGAATGAACCAGAATCT

[0860] TCAAATCTTGATTGGTCTTTGACAGAAGATGATTACAAGAAGATTTCCGAAATCTACC

[0861] AAGAGAGATTGATTAAAGGTCCAACCAAACCATTGCTGGATGATTTATGGGATGAAGA

[0862] ATAA

[0863] >SEQ ID NO:63

[0864] ATGGCTGCTTCTATCACTGCTATCACTGTTGAAAACTTGGAATACCCAGCTGTTGTTACT

[0865] TCTCCAGTTACTGGTAAGTCTTACTTCTTGGGTGGTGCTGGTGAAAGAGGTTTGACTAT

[0866] CGAAGGTAACTTCATCAAGTTCACTGCTATCGGTGTTTACTTGGAAGACATCGCTGTTG

[0867] CTTCTTTGGCTGCTAAGTGGAAGGGTAAGTCTTCTGAAGAATTGTTGGAAACTTTGGAC

[0868] TTCTACAGAGACATCATCTCTGGTCCATTCGAAGGTTGATCAGAGGTTCTAAGATCAG

[0869] AGAATTGTCTGGTCCAGAATACTCTAGAAAGGTTATGGAAAACTGTGTTGCTCACTTGA

[0870] AGTCGTGTTGGTACTTACGGTGACGCTGAAGCTGAAGCTATGCAAAAGTTCGCTGAAGC

[0871] TTTCAAGCCAGTTAACTTCCCACCAGGTGCTTCTGTTTTCTACAGACAATCTCCAGACG

[0872] GTATCTTGGGTTTGTCTTTCTCTCCAGACACTTCTATCCCAGAAAAGGAAGCTGCTTTG

[0873] ATCGAAAACAAGGCTGTTTCTTCTGCTGTTTTGGAAACTATGATCGGTGAACACGCTGT

[0874] TTCTCCCAGACTTGAAGAGATGTTTGGCTGCTAGATTGCCAGCTTTGTTGAACGAAGGTG

[0875] CTTTCAAGATCGGTAACTAA

[0876] >SEQ ID NO:64

[0877] ATGGCTGGTGCTGCTCCAGTTATTACTGCTATTCAAGTTGAAAACGTCGAGTTTCCACC

[0878] ATTGTATACTTCTCCAGCTACTGGTAAATCTTACTTTTTGGGTGGTGCTTGGTGAAAGAGG

[0879] TTTGGTTATTGAAGGTAATTTCATCAAGTTCACCGGTATTGGGTGTTTATTTGGAAGATAA

[0880] GGCTGTCGCTTCTTTAGCTCCAAAATGGAAAGGTAAATCCTTCGAAGAACTGAGAGAT

[0881] ACTTTGGAATTCTTCAGAGATATCATCTCCGGTCCATTTGAAAAATTGATCAGAGGTTCT

[0882] AAGATCAGGCCATTATCTGGTCCAGAATATTCTAAAAGGTCATCGAAAACTCCGTCGC

[0883] TCATATGAAATCTTGTGGTACTTATGGTGACGCTGAAGCTGCTGCTATTGAACAATTTGC

[0884] TCAAGCTTTTAAGAACGTCAACTTCCCACCAGGTGCTTCTGTTTTCTATAGACAATCTC

[0885] CAGATGGTATCTTGGGTTTATCTTTTTCTCAGGATGGTTCTATCCCAGAAAAAGAAGCTG

[0886] CTGTTATTGAAAACAAGGCTGTTTCTGGTGTTGTTTTAGAAACTATGATCGGTGAACAC

[0887] GCTGTTTCTCCAGATTTGAAAAGATGTTTGGCTGAAAGACTGCCAGTTTTATTGAATGC

[0888] TGGTACAACTAACTTCAAGATCGGTAATGGTAACTAA

[0889] >SEQ ID NO:65

[0890] ATGTCAAATATGGATCCTAATTCAGTTTTACCTAAGAGAAGTTTCTTACAACATGAGTTG

[0891] TTTTCACAATTACATATACCTGGAAGCTTAGCTTTCGAAGCATTTAGTTGTATCTCTAAGT

[0892] TTACAGGAGCTTTGCTTTGTTGGTTTTCACATGGAAACTTGCAAAGAAGTATCTAAA

[0893] CATCAATGGGGATTAACTTGTAAGAGCCGTGATTCTTTAAAACATGTGTTTGAACATAG

[0894] GAACGTCTCTGTGTTCCCATTTCACTATGTATCAAAAGATATTAGTCCTGGCTTCTTTTGG

[0895] AAACATATCTAAATCCACTATTCAGCATTTTGTTAATGAAGCTGAGAGGCTACATTCGTG

[0896] TTCTTTGCTCTCTTTGGCGGCTGCGATGATACCGTCTTTGAATGTCATGTCTGCAAATGG

[0897] ACTTGCTTCTGCCACTAGGGAGTAATGATGTTAAACTTAGGGAGAACATTGAACATAGGA

[0898] CTTGTCCGGAGAACACTGAACATAGGACTTGTCAGGTGGGATGTGAAGAATATAGTGG

[0899] TTTGAGTTTTCAAAAATTAGACTGGACAAGACAATCAGTGGAGCCTAGGACGGGGATC

[0900] GAGTTCCCGATGTTGTTGAAGGAGAATGCTTCGCGATCGAATTCTGAGGTGCTTGTTGC

[0901] AACGGGATCTCGGACAATGAAAATTATCAGAATTAAATCTCTAAAAGTATATGCATTTGG

[0902] TTTTTATGTTCACCCTTCCTCGGTCTGTCAGAAGCTTGGCCGAAAGTATGCCTCGGTTCC

[0903] AGCAAGCAAACTTGACAAATGCGATGACTTATACAAAGACCTTCTTAGGGAGGATATTG

[0904] TTATGAGTGTGAGGCTTGTGGTTAACTACAATGGCTTGAAGATCAATACTGTGAGAGAT

[0905] GTTTTCGAGAAATCTCTGCGTGCTCGCTTGGTTAAGGCAAATCCAAAAACTGATTTCAA

[0906] TTGTTTGAATGATTTTGGTTCATTCTTTAGACAAGACATTCCAATACCCGCGGGAACAAT

[0907] AATAGACTTTCGGCGAACAGAAGATGGACAGCTAATCACAGAGATTGGTGGTAACCTG

[0908] ATTGGAGCTGTCCGAAGCAAGGATCTTTGCAGAGCATTTTTTGGTATGTACATTGGAGA

[0909] TGTTCCGGTGTCAGAGCAGACAAAGGAGGAGATTGGTAGAAAAGTGGTGGGAATCAT

[0910] AAAGAGATGCTGA

[0911] >SEQ ID NO:66

[0912] ATGTTGGTGGAACTTGCAATTACTCTGTTGGTGATAGCCCTGTTCATACACCTGCGTCCC

[0913] ACACCAAGTGCAAAATCAAAGTCCCTTCGCCACCTTCCAAACCCTCCAAGTCCAAAAC

[0914] CCCGTCTCCCATTCGTGGGTCACCTTCACCTTTTAGACAAACCCCTTCTCCACAACTCC

[0915] CTCATCGATCTAAGCAAACGCTATGGTCCCCTTTACTCCCTCTACTTCGGTTCCATGCCT

[0916] ACCGTTGTAGTCTCCACCCTGAACTTTTCAAACTCTTCCTCCAAACCCACGAGGCCTC

[0917] TTCCTTCAACACCAGGTTCCAAACCTCTGCCATTAGGCGCCTAACCTACGACAACTCTG

[0918] TTGCCATGGTTCCCTTTGGTCCTTTACTGGAAGTTCATTAGGAAGCTTATCATGAACGATC

[0919] TCCTCAATGCCACAACTGTGAACAAGTTGAGGCCTTTAAGGAGCCAGGAAATCCGAAA

[0920] GGTCCTTAGGGTGATGGCACTGAGTGCAGAGTCTTCAAGTTCCTCTTAATGTTACCGAGG

[0921] AGCTTCTCAAGTGGACCAACAGCACCATCTCGAGGATGATGCTTGGGAAGCAGAGG

[0922] AAATCAGGGACATAGCACGTGACGTGCTTAAGATCTTTGGGGAGTATAGTCTCACCGAC

[0923] TTCATCTGGCCCTTGAAGAAACTCAAGGTTGGGCAATACGAGAGAGAGGATTGACGATA

[0924] TATTCAACAGGTTTGACCCCGTCATTGAGAGGGTCATCAAGAAAACAGGAGATTAG

[0925] GAAGAAGAAGAGGAGGAGGAATGGTGAGGTCGAGGAGGGGGAACAGTGTGGTTT

[0926] TTCTCGACACTTTGCTCGATTTTGCTGAGGATGAGACCATGGAGATCAAAATCACCAAG

[0927] GAACAAATCAAGGGTCTTGTTGTGGATTTCTTCTCAGCAGGGACGGATTCCACGGCCG

[0928] TGGCAACAGAATGGGCTCTGTCAGAGCTCATCAACAACCCCAGGGTGCTTCAAAAGGC

[0929] ACGAGAGGAGGTCGATGCGGTTGTGGGAAAAGACAGACTCGTTGACGAGGCAGATGT

[0930] CCAGAACCTTCCTTACATTAGATCCATCGTGAAGGAGACGTTCCGCATGCACCCACCAC

[0931] TACCCTTGGTCAAAAGAAAGTGCGTGCAGGAGTGTGAGATCGACGGTTATGCGATCCC

[0932] AGAGGGAGCATTGATCCTTTTCAATGTTTGGGCCGTCGGAAGAGACCCAAAATACTGG

[0933] GACAGGCCCACTGAGTTCCGTCCCGAAAGGTTCTTAGAAAATGTGGGTGAAGGGGATC

[0934] AAGCCGTTGACCTTAGGGGTCAACATTTCCAACTTCTTCCATTTGGGTCTGGAAGGAG

[0935] GATGTGCCCTGGTGTGAATTTGGCCACTGCGGGAATGGCCACACTGCTTGCGTCAGTTA

[0936] TCCAGTGCTTTGATCTCAGCGTGGTGGGCCCACAGGGAAAGATATTGAAGGGCAATGA

[0937] TGCCAAGGTTAGCATGGAAGAGAGTGCTGGACTCACGGTTCCAAGGGCACATAACCTC

[0938] GTGTGTGTCCCGGTTGCAAGATCAAGTGCCGTACCTAAACTCTTTTCCTCGTGA

[0939] >SEQ ID NO:67

[0940] ATGGCTTCCACAACACCCACCGCCTCCACCGACACTAAAACGATTGTTGCAGAGATTC

[0941] CTACCTATATCCGAGTCTTCAGTGACGGCACCGTTGAACGGCCACGACAAGCACCCAC

[0942] AGTGCCACCCATGCTCGATGACCCAAAAACAGGAGTCTCATCCAAAGACATCACCATC

[0943] TCACACCACCCCAAAATCTCCGCTCGCCTTTTCCTTCCAAAACTCAGCGACCGAGCCG

[0944] AAAAAGTTCCCATCTTGGTCTTCTTTCACGGAGGTGGATTCTTCTTCGAATCAGCTTTCT

[0945] CACAACTCTACCATGACCACTTCAACAGCTTCGTTTCACAAACAAATGTTCTAGCGGTT

[0946] TCAGTGGAGTACAGGCTGGCACCAGAACACCCTCTCCCTGCATGTTACCATGATTGTTG

[0947] GGATGCACTCCAATGGGTATCTTCCCATTCTACAAATAATAGCCCCAACATTAACAATAT

[0948] TGCAGAGCCATGGTTGATTGAGCATGGTGATTTCAACAGGGTTTTCATTGGGGGTGACA

[0949] GTGCTGGTGGTAACATCGTGCACAACATCGCCATGCGTGCTGGGGATGAGGCTTTACCT

[0950] TGTGATGTTAATATATTGGGGGCTATTTTTGCACACCCTTATTTCTACGGCTCGTCCCCAA

[0951] TTGGGTCAGAACCTGTTGAAAAGCGTGAAGAGGAGTTGTGCTATTTGGTTTGGAAATT

[0952] GGTGTATCCTTCTGCGCCTGGTGGGATTGATAACCCCATGGTCAACCCTTTGGGTCCAG

[0953] GGGCACCTAGTCTTGCTGGGCTTGGGTGTTCTAAGATCATTGTGTGTGTTGCTGGGAAG

[0954] GACAAAATTAGGGATAGAGGGGTTTGGTACTATGAGGCTGTGAAGAAGAGTGGGTGGA

[0955] AAGGGGAAATTGAGCTCTTTGAAGAGAAAGAGGAGGACCATGTTTATCATATCTTTCAT

[0956] CCGGAATCTGAGAATGGCAAGAAGTTGATCAAACGCTTGGCTTTGTTTCTCCACGAGT

[0957] GA

[0958] SEQ ID NO:68

[0959] ATGGTTCTAATAATGGTAGAAAAGCTTCTGAAATTTTTCAAGGTCAAGCTTTGTTGTA

[0960] CAGACATATGTTTGCTTTCGTTGATTCTATGTGTTTGAAGTCTATTGTTGAATTGGGTATT

[0961] CCAAATATCATTCATAAACATGGTCAACCAATTACTTTGTCTGAATTGATGTCTATTTTGC

[0962] AAGTTCCACCTGCTAAAGTTGGTCATGTTCAATCTTTGATGAGATATTTGGCTCATCATG

[0963] GTTTTTTTGAAAGATTGAGAATTCATGAAAGAGATGCTTATGCTTTGACTGCTGCTTCTG

[0964] AATTGTTGGTTAAAGGTACTGAACCATATTTGGCTCCAATGGTTGAATGTATGTTAGATC

[0965] CAACTTTGTCTGCTTCTTTCATCAAATGAAAAAATGGGTTTACGAAGAAGATTTGTCT

[0966] GTTTTTGATATTCTTTGGGTTCTAATTTGTGGGATTTTTTGACTAAAAATCCTGCTTATA

[0967] ATGAAATTTTTAATGAAGCTATGGCTTCTGATTCTCAAATGTCTAATTTGGCTTTGAGAG

[0968] ATTGTAAATTGGTTTTTGAAGGTTTGGAATCTATTGTTGATGTTGGTGGAGGTACTGGTA

[0969] CTACTGCAAAATTATTGTGAAGCTTTTCCAAATTTAAAATGTATTGTTTTTGACAGAC

[0970] CTCAAGTTGTTGAAAATTGTTGGAAAATAATAACTTAACTTATGTTGGTGGAGACATG

[0971] TTTAAATCTATTCCAAAAGCTAATGCTGTTTTGTTGAAATGGATTTTGCATGATTGGACA

[0972] GACAAAGACTGCAGAAAAATCTTGGAAAATTGTAAAGCTATTTCTAATAATTCTAA

[0973] AAGAGGTAAAATTATCATTATTGATATTGTTATCAATGAAAAACAAGATGAACATAAAGT

[0974] TACTGAATTGAAATTGTTATTGGATGTTGCTATGGCTTGTGTTTTGAATGGTAAAAGAAAG

[0975] AAATGAAGAGGAATGGAAAAAATTGTTTATGGAAGTTGGTTTGCAAGATTATAAAATTT

[0976] CTCCCATTGACTGGTTATTTGTCTTTGATTGAAATTTATCCATAA

[0977] SEQ ID NO:69

[0978] ATGGACTTCTCTACTAACGGTTCTGAAGAATCTGAATTGTACCATGCTCAAATCCATTTG

[0979] TACAAGCATGTTTACAACTTCGTCAGTTCTATGGCTTTAAAGTCTGCTATGGAATTGGGT

[0980] ATTGCTGATGTTATTCACAACCATGGTAAACCAATCACTTTGCCACAATTAGCTTCTGCT

[0981] TTGAAATTGCATCCATCTAAAGTCGGTGTTTTGTATAGATTCCTGAGATTGTTGACCCAT

[0982] AACGGTTTCTTTGCTAAAACAACTGTCCCATCTCAAAACGGTAAAGAAGGTGAAGAAG

[0983] AAGAAACTGCTTACGCTTTAACTCCACCATCTAAATTGTTGGTTAAGGGTAAACCAACC

[0984] TGTTTAGCTTCTATTGTTAGGGGTGCTTTGCATCCATCATCTTTGGATATGTGGAGATCTT

[0985] CTGAAAAGTGGTTTAACGAAGACAAGGAATTGACTTTGTTCGAATCTGCTACAGGTGA

[0986] ATCTTTTTGGGATTTTCTAAACAAGGACTCCGAATCTGGTACATTATCTATGTTTCAGGA

[0987] GGCTATGGCTGCTGATTCTCAAATGTTTAAACTGGCTTTGAAGGAGTGTAGACATGTTT

[0988] TTGAAGGTTTGGAATCCTTGGTTGATGTTGGTGGTGGTACAGGTGGTGTTACAAAATTG

[0989] ATTCATGAGGAATTCCCCCATTTGAAATGTACTGTTTTCGATCAACCCCAAGTTGTTGGT

[0990] AATTTGTCTGGTAATGAGAACTTGAAGTTCGTTGGTGGTGACATGTTTAAATCTATCCCA

[0991] CCAGCTGATGCTGTTTTATTGAAATGGGTTTTGCACGATTGGAACGATGAATTATCTTTG

[0992] AAGATCCTGAAGAACTCCAAGGAAGCTATTTCTGGTAAAGGTAAAGAGGGTAAAGTCA

[0993] TTATCATCGATATCTCTATCGACGAAGCTTCTGGTGACAGAGAATTAACTGAATTACAGT

[0994] TGGACTACGACTTGGTTATGTTGACAATGTTTAACGGTAAAGAGAGGGAAAAGAAGGA

[0995] ATGGGAAAAATTGATCTCCGATGCTGGTTTTTCTTCTTATAAGATCACCCCAATCTGCGG

[0996] TTTTAAATCTTTGATTGAGGTCTTCCCATAA

[0997] SEQ ID NO:70

[0998] ATGGAAATTTTGTCTTTGTTATCTTATTCTGTTTTTTATTTGGCTTTATTTTTTATCTTTAAT

[0999] ATTGTTATTAGAGCTAGAAAATTTAAAAATTTGCCACCTGGTCCACCATCTTTGCCAATT

[1000] ATTGGTAATTTGCATCATTTGAAAAGACCATTGCATAGAACTTTTAAAGGTTTGTCTGAA

[1001] AAATATGGTCATGTTTTTTCTTTGTGGTTTGGTTCTAGATTGGTTGTCGTTGTTTCATCTG

[1002] CATCAGAATTTCAACAATGTTTTACTAAAAATGACGTTGTCTTGGCAAATAGGCCAAGA

[1003] TTTTTGTCTGGTAAATATATTTTTTATAACTACACTACTTTGGGTTCTACTTCTTATGGTGA

[1004] ACATTGGAGAAATTTGAGAAGGATTACTGCATTGGATGTTTTGTCTAATCATAGGATTAA

[1005] TTCTTTTTCTGGTATTAGAAGAGATGAAACTCAAAGATTGATTACTAGATTGGCTGATGA

[1006] TTCTTCTACTAATTTTGCTGAAATGGAATTGTCATCTAGATTGTATGATATGACTTTTAAC

[1007] AATATCATGAGGATGATTTCTGGTAAAAGATATTATGGTGAAGATTGTGATACTTCTGATT

[1008] TGCAAGAAGCTTCTCAATTTAGAGATATGGTTTCTGAATTGTTGCAATTGTCTGGTGCTA

[1009] ATAATAAGACTGATTTTATGCCATTATTGAGATTTTTGGATTTTGAAAACTTGGAAAAAA

[1010] GATTAAAAGATATTTCTGGTAAAACTGACGCTTTTTTGAGAGGTTTAATTGAAGAACAT

[1011] AGAACTAAGAAGGAAAGAGCTAATACTATGATTGATCATTTATTGAATTTGCAAGATTCT

[1012] CAACCTGAATACTATACTGATCAAATTATTAAAGGTTTGGCTTTGGCTATGTTGTTGGCT

[1013] GGTACTGATTCTTCTGCTGTTACTTTGGAATGGTCTATGTCTAATTTGTTGAATCATCCTG

[1014] AAGTTTTGAAAAAAGTTAAAGATGAATTGGATACTCATGTTGGTCAAGATAGATTGGTT

[1015] GATGAATCTGATTTGCCAAAGTTAACTTATTTAAAAAACGTCATTAATGAAACTTTGAG

[1016] ATTGTATACTCCTGCTCCATTGTTATTGCCACATTCTACTTCTGATGAATGTAATATTGGT

[1017] GGTTATAAAGTTCCACAAGATACTATTGTTTTGATTAATGCTTGGGCTATTCATAGAGATC

[1018] CTGAATTGTGGACTGAAGCTACTACTTTTAAACCTGAAAGATTTGAAAAAAAGGTGA

[1019] ATTGGAAAAATTGATTGCTTTTGGTATGGGTAGAAGAGCTTGTCCTGGTGAAGGTTTGG

[1020] CTATTAGAGCTATTTCTATGACTTTGGCTTTGTTGATTCAATGTTTTGATTGGAAATTGAT

[1021] TAATGGTGATAAAATTGATTTGGCTGAAAGAGATGGTTTTACTTTGACTAAATTGGTTCC

[1022] ATTGAAAGCTATGTGTAAATCTAGACCTGTTATTAATAAAGTTTTTAAACAATAA

[1023] >SEQ ID NO:71

[1024] ATGGAAATTTTATCTTTGTTGTCTTATTCTGTTTTTTATGTTGCTTTGTTCTTTATTTGTAA

[1025] TTTATTGTTTCAAGCTAGAAAATTTAAAAATTTGCCACCTGGTCCACCATCTTTGCCAAT

[1026] TATTGGTAATTTGCATCATTTGAAAAGACCATTGCATAGAACTTTTAAAGGTTTGTCTGA

[1027] AAAATATGGTCATGTTATTTCTTTGTGGTTTGGTTCTAGATTGGTCGTCGTTGTTTCTTCT

[1028] TTGTCTGTTTTTCACAGAGTGTTTCACAAAGAATGATATTGTTTTAGCTGATAGGCCAAG

[1029] ATTTTTGTCTGGTAAATACATTTTTTACAATATACTACTGTTGGTTCTTCATCTTATGGTG

[1030] AACATTGGAGAAATTTGAGAAGAATTACTTCTTTGGATGTTTTGTCTACTCATAGAATCA

[1031] ATTCTTTTTCTGGTGTTAGAAGAGATGAAACTCAAAGATTGATTCAAAAATTGGCTGAA

[1032] GAATCTTCTACTGATTTTGCTGAAATCGAGTTGACTTCTAAGTTCTATGATATGACTTTTA

[1033] ATAATATCATGAGAATGATTTCTGGTAAAAGATATTATGGTGATGATTGTGATATGATGGA

[1034] TATGGAAGAAGCTAAACAATTTAGAGCTATGGTTTCTGAATTGTTGCAATTGTCTGGTG

[1035] CTAATAATAAGACTGATTTTATGCCTGTTTTAAGGTTGGTCGATTTTGAAAATTTGGAAA

[1036] AAAGATTGAAAAAAATTTCATCTAAAACTGATACTTTCTTAAGAGGTTTGATTCAAGAA

[1037] CATAGAAATAAGAAACAACATACTAATACTATGGTTGATCATTTGTTGTCTTTGCAAGAA

[1038] TCTCAACCTGAATATTATACTGATCAAATTATTAAAGGTTTGGCTTTGGGTATGTTGTTGG

[1039] CTGGTACTGATTCTTCTGCTGTTACTTTGGAATGGGCTTTGTCTTGTTTGTTGAATCATC

[1040] CTGAAGCTTTGAAAAAAGCTAGAGATGAATTGGAAACTCATGTTGGTCAAGACAGATT

[1041] GTTGGAAGAATCAGATTTGACTAAATTACCATATTTGAAAAATATTATCTATGAAACTTT

[1042] GAGATTGTATACTCCTGCTCCATTGTTATTGCCACATTCTTCATCTGATGAATGTATTATT

[1043] GGTGGTTTTAAAGTTCCAAGAGAAACTATTATTTTGATTAATGCTTGGTCTATTCATAGA

[1044] GATCCACAAATTTGGTCTGAAGCTACTTCTTTTAAACCTGAAAGATTTGAAGAAGAAG

[1045] GTGAATTGGATAAATTGATTGCTTTTGGTTTGGTAGAAGAGCTTGTCCTGGTGAAGCT

[1046] TTGGCTTTGAGAGGTATTTCTTTGACTTTGGGTTTGTTGATTCAATGTTTTGAATGGAAA

[1047] TTGGTTGGTGATAAAGAAATTGATATGAGAGAAGAATCTGGTTTTACTTTGTCTAGATTG

[1048] ATTCCATTGAAAGCTATGTGTAAAGCTAGACCTGTTGCTAATAAATTGGTTAATACTCAA

[1049] GCTGTTTTCTTTGGGTGATTAA

[1050] SEQ ID NO:72

[1051] ATGGCTACTGAAAATAAAATTTTGATTTTGGGTCCAACTGGTGCTATTGGTAGACATATT

[1052] GTTTGGGCTTCTATTAAAGCTGGTAATCCAACTTATGCTTTGGTTAGAAAAACTCCTGGT

[1053] AATGTTAATAAACCAAAATTGATTACTGCTGCTAATCCTGAAACTAAAGAAGAATTGAT

[1054] TGATAATTATCAATCTTTGGGTGTTATTTTGTTGGAAGGTGATATTAATGATCATGAAACT

[1055] TTGGTCAAAGCAATTAAGCAAGTTGATATTGTTATTTGTGCTGCTGGAAGATTATTGATT

[1056] GAAGATCAAGTTAAAATTATTAAAGCAATCAAAGAGGCTGGTAATGTCAAAAAATTCTT

[1057] TCCATCTGAATTTGGTTTGGATGTTGATAGACATGATGCTGTTGAACCTGTTAGACAAGT

[1058] TTTTGAAGAAAGCTTCTATTAGAAGAGTTATTGAAGCTGAAGGTGTTCCATATACTT

[1059] ATTTGTGTTGTCATGCTTTTACTGGTTATTTTTTGAGAAATTTGGCTCAATTGGATGCTAC

[1060] TGATCCACCAAGAGATAAAGTTGTTATTTTGGGTGATGGAAACGTTAAAGGTGCATATG

[1061] TTACTGAAGCTGATGTTGGTACTTTTACTATTAGAGCTGCTAATGATCCAAACACTTTGA

[1062] ATAAAGCTGTTCACATTAGATTGCCAAAAAAATTATTTGACTCAGAATGAAGTTATTGCTT

[1063] TGTGGGAAAAAAAAATTGGTAAAACTTTGGAGAAAACTTACGTCTCTGAAGAACAAG

[1064] TTTTGAAGGATATTCAAGAATCTTCTTTTCCTCATAATTATTTATTAGCTTTGTATCATTCT

[1065] CAACAAATTAAAGGTGATGCTGTTTATGAAATTGATCCTGCTAAAGATATTGAAGCTTCT

[1066] GAAGCTTATCCTGATGTTACTTATACTACTGCTGATGAATATTTGAATCAATTTGTTTAA

[1067] >SEQ ID NO:73

[1068] ATGAACATGATCGAAGTTGGTGGTTATCCAGGTAATCCAATTAATACTAAACAGTCTAAC

[1069] GCTCAACAACATAAACCAACAGCTTCTTTGACTACATATCAAAATAAGAAGAAGAAGA

[1070] ACACCCATCCATCTTTCTTTAATTCTCCATATTTCTACTTCAACTTCAACATTCCAATGGC

[1071] TGCTGAAAATAAGATTTTAATTCTGGGTCCAACTGGTGCTATTGGTAGACATATTGTTTG

[1072] GGCTTCTGTTAAAGCTGGTAATCCAACATTTGCTTTGGTTAGAAAAACAAATGGTCCAG

[1073] TTAATAAGCCAAAATTAATCACAGCTGCTAATCCAGAATCTAAAGAACAATTGTTGGAA

[1074] TCTTACCAAAACGCTGGTGTTACATTATTGGAAGGTGACATTAATGATCATGAATCTTTG

[1075] GTTAAGGCTATTAAGCAAGTTGATGTTGTTATTTGTGCTACAGGTAGATTATTAATCGAT

[1076] GATCAAGTTAAGATCATCGCTGCTATTAAGGAAGCTGGTAATGTTAAAAGATTCTTTCCA

[1077] TCTGAATTTGGTTTGGATGTTGATAGACATGATTCTGTTGAACCAGTTAGAGAAGTTTTT

[1078] GAAGATAAAGCTAGAATCAGAAGAGTTATTGAAGCTGAAGGTGTTCCATATACTTATTT

[1079] GTGTTGTCATGCTTTTACCGGTTATTTTCTAAGAAATTTGGCTCAATTGGACGCTACTGT

[1080] TCCACCAAGAGATAAAGTTGTTATTTTGGGTGACGGTAATGTTAAGGGTGCTTATGTTAC

[1081] AGAAGCTGATGTTGGTACTTATACTATTAGAGCTGCTAATGATCCAAGAACATTGAATAA

[1082] GGCTGTTCATATTAGATTGCCAGCTAATTATTTGACTGCTAATGAAGTTGTTTCCTTATGG

[1083] GAAAAGAAAATTGGTAAAACACTGGAAAAGACATACGTTCCAGAAGAAGAAGTTTTA

[1084] AAGGATATCCAAGAGTCTTCTTTTCCACATAATTATTTGTTGGCTTTGTACCATTCTCAAC

[1085] AAATTAAGGGTGACGCTGTTTATGAAATTGATCCAGCTAAAGATGTTGAAGCATATGATT

[1086] TGTATTCCGATGTTAAATACACCACTGCTGATGAATATTTGAATCAATTTGTCTAA

[1087] SEQ ID NO:74

[1088] ATGGCTGAAGGTAAGGGTAGAGTTTGTGTTACTGGTGGTACTGGTTTCTTGGGTTCTTG

[1089] GATCATCAAGTCTTTGTTGGAAAACGGTTACTCTGTTAACACTACTATCAGAGCTGACC

[1090] CAGAAAGAAAGAGAGACGTTTCTTTCTTGACTAACTTGCCAGGTGCTTCTGAAAAGTT

[1091] GCACTTCTTCAACGCTGACTTGTCTAACCCAGACTCTTTCGCTGCTGCTATCGAAGGTT

[1092] GTGTTGGTATCTTCCACACTGCTTCTCCAATCGACTTCGCTGTTTCTGAACCAGAAGAA

[1093] ATCGTTACTAAGAGAACTGTTGACGGTGCTTTTGGGTATCTTGAAGGCTTGTGTTAACTC

[1094] TAAGACTGTTAAGAGATTCATCTACACTTCTTCTGGTTCTGCTGTTTCTTTCAACGGTAA

[1095] GGACAAGGACGTTTTGGACGAATCTGACTGGTCTGACGTTGACTTGTTGAGATCTGTT

[1096] AAGCCATTCGGTTGGAACTACGCTGTTTCTAAGACTTTGGCTGAAAAGGCTGTTTTGGA

[1097] ATTCGGTGAACAAAACGGTATCGACGTTGTTACTTTGATCTTGCCATTCATCGTTGGTAG

[1098] ATTCGTTTGTCCAAAGTTGCCAGACTCTATCGAAAAGGCTTTGGTTTTGGTTTTGGGTA

[1099] AGAAGGAACAAATCGGTGTTACTAGATTCCACATGGTTCACGTTGACGACGTTGCTAG

[1100] AGCTCACATCTACTTGTTGGAAAACTCTGTTCCAGGTGGTAGATACAACTGTTCTCCAT

[1101] TCATCGTTCCAATCGAAGAAATGTCTCAATTGTTGTCTGCTAAGTACCCAGAATACCAA

[1102] ATCTTGACTGTTGACGAATTGAAGGAAATCAAGGGTGCTAGATTGCCAGACTTGAACA

[1103] CTAAGAAGTTGGTTGACGCTGGTTTCGACTTCAAGTACACTATCGAAGACATGTTCGAC

[1104] GACGCTATCCAATGTTGTAAGGAAAAAGGGTTACTTGTAA

[1105] >SEQ ID NO:75

[1106] ATGGCTGAAGGTAAAGGTAGAGTTTGTGTTACAGGTGGTACTGGTTTTCTAGGTTCTTG

[1107] GTTAATTAAGAGGCTGTTGGAAGATGGTTACGGTGTTAATACAACAATCAGATCTGACC

[1108] CAGAAAGAAAGAGATATCTCTTTCCTGACCAACTTGCCAGGTGCTTCTGAAAGATT

[1109] GAAAATTTTCAACGCCGACTTGTCTGATCCAGAATCTTTTGGTCCAGCTATTGAAGGTT

[1110] GTGTTGGTGTTTTTCATACCGCTTCTCCAATTGATTTCGCTGTTTCTGAACCAGAAGAA

[1111] ATTGTTACTAAGAGGACTGTTGACGGTACATTGGGTATTTTGAAAGCTTGTAAGAACTC

[1112] CAAGACCGTTAAGAGTTGTTTACACATCCTCTGGTGCTGCTGTTTCTTGGGGTGGTA

[1113] CTGAAAAAGATGTTTTGGATGAATCCGACTGGTCTGATGTTGATATGTTAAGATCTGTCA

[1114] AGCCATACTCTTGGTCTTATGCTGTTTCTAAAACCATGACAGAGAAGGCTGTTTTAGAA

[1115] TTTGGTGAACAACACGGTTTGGATGTTGTTACATTGATTTTGCCATTCATCGTCGGTAGA

[1116] TTCATTTGTCCAAATCCCAGATTCCGTTGAAAAAGCTTTGGTTTTAGTCTTGGGTAA

[1117] AAGGGAACAAATCGGTGTTACAAGATTCCATATGGTTCATGTTGAGGATGTTGCTAGAG

[1118] CTTATATTTTCTTGCTGGAACATCCAAACCCAAGGTAGATATAACTGTTCTCCATTCA

[1119] TCGTTCCAATCGAAGAAGTTTCTGAATTGTTGTCTGCTAAGTACCCAGAATATCAAATC

[1120] CCAACTGTTGAAGAACTGAAGGAAATTAAGGGTGCTAAATTGCCAGATTTGAAGTCTA

[1121] AAAAGCTGGTTGATGCTGGTTTTGAATTCAAATACACCATCGAAGACATGTTCGATGAT

[1122] GCTATTCAATGTTGTAAGGAGAAGGGTTATCTGTAA

[1123] SEQ ID NO:76

[1124] ATGGCATCTCAACATAAAAAGAAGACTCAGATTGAATATAATGTTTTGAGATTTCAAAG

[1125] ACCATCTTTGGGTCATGGTTATAATTTTTCTGGTGGAGGTGGATCTACTTATCAAGAATG

[1126] TAATAGAAAATATGCTGTCAAGGCTGTTTATGATCAGCCAAAAGATTTTGAATTGGAAG

[1127] CTTCTAACCCAAAAAATATTTTGGATTCAGCTAAAAAATTTTTGGCTGCATTTTATTATTT

[1128] CTCATATCCATACACTATGATTGGTATTACTTTGTGTGCTTTTTGTTCTTCTGCTTTGGCT

[1129] GTTGAAAAATTGTCTGATATTTCTTTGCCATTTTTTATTGGTGTTTTGCAAGCTGTTATTC

[1130] CACAATTGTTTATTGAAATTTATTTGTCTGGTGTTAATCAATTGTATGATTTAGAAATCGA

[1131] CAAAATTAATAAACCACATTTGCCAATGGCTTCTGGTCAATTTTCTTTTAAAACTGGTGT

[1132] TATTATGTCTGCTGCTTTTTTGGCTTTGTCTTTTGGTTTTACTTGGTTGACTGGTTCTTGG

[1133] CCATTGATTTGGAATTTGGTTGTTATTGCTTCTTCTTGGACTGCTTATTCTATTGATGTTC

[1134] CATTTATGAGATGGAAAAGATATCCATTGGTTGCTGCTATGTGTATGATTGCTACTTGGG

[1135] GTTTGGCTTTGCCAATTTCTTTTTTTCATCATATGCAAACTTTTGTTTTGCAAAGACCAA

[1136] TTGGTTTTCCAAGATCTTTGGGTTTTTTGGTTGCTTTTATGACTTTTTATTCTATGGGTATT

[1137] GCTTTGTCTAAAGATATTCCTGATGTCGAAGGAGACAAGGAACATGGTATTAATTCATTT

[1138] TCTGTTTTGTTGGGTCAAAAAAGAATTTTTTGGATTTGTGTTTCTTTGTTTTGAAATGGCT

[1139] TTTGGTGTTGGTTTGTTGGGTGGTGTTACTTCTCCACATTTTTGGACTAAAATTATTACT

[1140] GGTTTGGGTAATGTTATTTTGGGTTCTATTTTGTGGTATCAAGCTAAGTCTTTTGATTTGA

[1141] CTGATAAAGCTTCAACTGGTTCATTTTATATGTATATTTGGAAATTGTTGTATGCTGCTTT

[1142] GTTATTGATGGCTTTGTTAGATAA

[1143] >SEQ ID NO:77

[1144] ATGGATTCAATGGTAGTTGGCTCCTTTCCAAATGCATCCTCAATAACGACTGGGGGTAA

[1145] CCTATGGCTAGGCAAAAACTGTGCCAAAAACTACTACGCAACCTCTTTCTACACTCCCA

[1146] AAGCGTCTCAACATAAGAGAAAAATTCAGAAAGAATACAATTTTCTAAGGTCTAGGCA

[1147] GCCCAGCCTAAAGCATTTATACAAAGGTATTGAAGGTGGATCAAACCTACCAAGAATGCA

[1148] ACAGAAAGTATGTGGTTCAGACCGCCCCAAAGCCCTCTTTCGAAAGTAATCCGCGTTC

[1149] CATAGATCAGAAGTATAACCTAGAGTCAGTTAAAACATTTCTAGATGCATTCTATATGTT

[1150] CTGTACCCCATACGCGTTCATGTGCACAGTGCTGTATATAGCAAGCATGAGCTTATTGGC

[1151] GGTCGAGAAGCTGTCTGACATTAGTCCGTTGTTCTTTATTGGTATTCTACAAGCTTATGT

[1152] TCCATATTTGTTCATTCATATCTACGTTACCGGTTTAAATCAGGTCTGTGATGTCGAAATA

[1153] GATAAAATTAATAAGCCATATTTACCATTGGCTAGCGGTAAGATATCCTTTACCACAGGT

[1154] GTGATTATTGTGGTTTCATGTCTAATCTTGGTACTGTATATTTTCATCCCGTTTTACAATGA

[1155] CAACTTTCAGGATGATTTCACGAATAGCGTTGAAGGTGGTCTTTTGTTTTATTACTTTGT

[1156] CCAAGGTTTATGGCTAAGGCTTATCGTCGGCTCTTGGCCCTCAACTTGGGCATTGATTTC

[1157] TTTAGTCATGACCTGGGGTGCCTATTCAATCAACGTTCCACTTCTTAGATGGAAAAAGA

[1158] ATCCAGTTCTGGCTGCCATGTGTAATTTTCTGACTTTTGGCGTCACATTTCCTCTAGGTT

[1159] ACTTTTTTCATATGCAAACTTTCGTCTTTAAAAGGCCATCTTTTTTTCCGAGACCTTTAAT

[1160] CTTTGCGACTACTATCATGTCTTTCTTCAGCTTGGTAATTGCATTGTTTAAAGACCTGCC

[1161] GGACATTGAAGGCGACCAAGCATTTGGTGTGAAAACCTTTGCAACCAGATTCGGACAA

[1162] AAATGGGTGTTCTGGATTTGTATTTCCCTATTGGAGATGGCCTACGGTGTTGCAATTTTG

[1163] ATGGGTGCTACGAGCTCCTTTTTATGGAGTAAAATTGTGACCGGATTAGGGCATGCTATT

[1164] TTAGCTAGGTTTCTTGCTATATAGGGCCAAGTCCATCGATCTACGTTCTAAGGCCTCAATT

[1165] GCTAGTTTTTACAAACTAATCTGGAAGTTGATGTGTGTTGAATGTTTCTTGATGCCTTTG

[1166] GTGAGGTAA

[1167] >SEQ ID NO:78

[1168] ATGTCATCCCTATTTGGTAGTAACGTGTTTGAATCAATTTCTACATATAGAAGTTATGCTC

[1169] CTAAGGCCAGTCAGCACAAAAGGAAGATCCAGAAAGAATATAATTTTCTGAGGTCTAG

[1170] ACAGCCTAGCCTGAAGCATATATATAAAGGAATTGAGGGTGGATTCTACCCAGCAAGAAT

[1171] GCAATAGAAAATATGTAGTTAAGACGGTGCCCAAACCTAGTTTTGAAAGTGATCCTAGA

[1172] TCAATAGACCAGAAAGACATATTAGAGTGTATAAAAAATTTTCTAGATGCCTTGTACATG

[1173] TTCACAACTCCTTACTCTTTCTTCTCTTCTGCATTGTATATCATTTCTGTATCTTTCATTGT

[1174] GGTTGAAAAATTATCTGACATCTCACCTCTGTTTTTCACCGGCGTTTTACAAGCCGTTGT

[1175] TCCAAATCTTTTCGTGCATATATACATGACAGGTTTGAACCAATTGTGCGATGTGGAAAT

[1176] TGATAAAATTAATAAGCCGTACTTACCTTTGGTTTCCGGTAAAATTTCCTTCGCAACAGG

[1177] TGTTATCGTAGTAGCTAGCTGTCTGTTCTTAAGTCTGTGGCTGGGTTGGATTGTAGGTTC

[1178] CTGGCCATCCACTTGCGTGGTGATATCAATTGCGATGATCTGGACAGCTTACTCTATTAA

[1179] CGTACCTCTTTTGAGGTGGAAAAAGTACCCTGTCTTAGCGGCCATGTGTATTTTTTTAAG

[1180] TCTTGCAGTGGTGAATCCCATTGGCTACTTCTTACACATGCAGACGTTTGTCTTCAAAA

[1181] GACCTGCGTCATTCAGTCGTCCCTTGATATTTGTCATTGCTTTTAAATCCTTTTTCTCATT

[1182] AGCAGTGGCTTTATTTAAAGATGTGCCGGATATAGAGGGAGACCAAACTTTCGGCGTGC

[1183] AAAGCTTCGTCGCTAGGTTTGGTAAGAAAAGGGTCTTCTGGATTTGCATTTCTCTAATC

[1184] GAAATGGCGTACGGTTTTGCGTTGTTAATGGGGGCGACGTCTGCTTTCCTTTGGTCCAA

[1185] AATCGTTACCGTGTTAGGACACGCAGTTTTGGCATCTTTTGTGCTATATAGAGCGAAGTC

[1186] CATTGACCTTAGGTCTAAGACCTCTATCACTAGTTTCTACATGCTTATATGGAAACTGAT

[1187] GTACATGGAATACTTTTTAATGCCTTTGGTTAGGTAA

[1188] >SEQ ID NO:79

[1189] ATGGGTTCTGTTGCCGTTGTAAGCTTCCCAAATGCATCATCTATAACTATCGGTGGTAAC

[1190] TTGTGGAGGGGCAAAAACTGTGTGAACAACTATTACGCTGCAAGCTCATATACGCCGA

[1191] AAGCCTCTCAACATAATAGAAAAATCGAAGAAGAAAAATAATTTCCTTTCCAGTCAGCA

[1192] GTTAAGCTTGAAGCATCTTTATAAGGGAATAGAGGGAGGTAGTACATACCAAGAATGTA

[1193] ATGGGAAGAATGTCGTTAAGATAGCCCCTAAACCTTCATTTGAAAGCGACAGCAGGAG

[1194] CATTGAGAGGAAGAATATAATGGAAAGTATTAAGAATTTCCTGACGTTTTCTATATGTT

[1195] CTGCACTCCTAGGTCATTTATTGCGCCAGCTTTCGGTGCCATCTCAAGTTGTTTATTGGT

[1196] GGTAGAAAAACTATCCGATATCTCACCTTTATTTTTCATAGGTGTCTTGCAGGCCGTTAT

[1197] CCCTTATTTGTTTATTAGTGTTTATGTCCACGGTATTAATCAGTTGGGGGATGTAGAAATC

[1198] GACAAGATCAACAAGCCATACCTACCACTTGCATCAGGTAAACTGTCATTTACCACGGG

[1199] TGTTATCATAGTGGCGTCTTGTTTAATTCTTGGCTTGGGTTTGGGGTGGATTGTTGGTTC

[1200] ATGGCCACTGATCTGGTCTCTTATTCTATCATCAGTTATTTGTGCAGCATACTCAGTTAAC

[1201] GTTCCGCTACTTAGATGGAAGAGGAATCCAGTTTTGGCTGCAATGTGTACTGTTGTTAA

[1202] TCTAGGCTTCGTTTTGCCAATAGCAAATTTCCTTCATATGCAAACTTTTGTGTTCAAGAG

[1203] ACCTGCTTTTTTCAGTAGACCTCTTATCTTCGCTACTACCTTCATGGGATTATATAGCTTA

[1204] GTGATCGCTTTGTTCAAGGATATCCCAGACATTGAAGGTGACCAGGCATTTGGTGTACA

[1205] AACCTTCGCTGCCTGTTTTGGACAAAAGAGAATATTCTGGATCTGTATTTCACTGCTTG

[1206] AAATGGCGTACGGTGTCGCAATGTTAATGGGTGCTACCTCATCCTGTCTGTGGAGTAAA

[1207] ATAGTTACTGGATTGGGACATGCTGTATTAGCGCTTGTCGTTTTGTATAGAGCACGTAGC

[1208] GTTGATCTGACCTCTAAAACTTCATTGTCATCATTCTATATGTTGATCTGGAAGTTAGTGC

[1209] ATGTTGAATACTTGTTAATGCCTTTAGTGAGGTAA

[1210] SEQ ID NO:80

[1211] ATGGACAGTGTCATAGTTGGTTCCTTTCCAAATGCAAGCTCAATTACCACAGGAGGTAA

[1212] TTTATGGCAAGGTGAAAATTGCGCAAAGAACTACTACGCAACCTCATCCTACACTCCAA

[1213] AGCTAGTCAACATAAAAGGAAAATACAAGAATATAATTTCCTTCGTTCTAGGCAG

[1214] CCCTCCCTTGAAACATTTATATAAGGGTATTGAAGGTGGTAGCACATACCAAGAATGCAA

[1215] CGGTAAGTATACTATGAAGACCGCACCAAAACCAAGCTTTGAGTCTGACCCCAGATCC

[1216] ATAGACCAAAACAATATTTTGGAGAGCACTAAGACCTTCTTGGATGCTTTTTACATGTTT

[1217] TGTACTCCATCGCGTTAATTTGTAGCGTATTATTCATAATTTCCATGTCATTGTTGGCCG

[1218] TTGAGAAGTTGAGCGACATCAGCCCCTTATTTTTTACTGGGGTTCTACAAGCACTGGTT

[1219] CCTCATATGTTTGTCGTTATATATATCACTGGAATCAATCAAGTTTGTGATGTTGAGATCG

[1220] ATAAAATTAACAAGCCCTACTTGCCCCTAGCGTCTGGAAAGATATCTTTCACAACATCT

[1221] GTCATAGTTGTAGCTAGTTCATTGATCACGGGATTAAGTTTGTCATGGATTGTTGGAAGT

[1222] TGGCCTTCCACTTGGGCTCTAATTTCTTTGACAGTCATATGGGGCGCCTACAGCTTGAAT

[1223] GTCCCACTATTGAGATGGAAGAAAAACCCTGTCCTTGCCGCCATGTGTCAGTTTTTAAC

[1224] AACCGCAGTCGTTAATCCAATAGGCTACTTCCTGCACATGCAAACTTTTGTGTTAAAAC

[1225] GTCCGATTTTTTTTTCTCGTCAGTTGATCTTTGCTATTGCCATAATGTCTTGTTTTTCACT

[1226] AGCAGTCAGCCTATTCAAGGACTTGCCAGATATTGAAGGTGATCAGGCATTTGGAATAC

[1227] AGACATTTGCTGCAAGATTTGGACAAAAAAGAGTGTTCTGGATATGCATCTCTTTGTTA

[1228] GAAATGGCTTACGGAGTAGCGCTTTTGATGGGTGCCACAAGTTCTTGTTTGTGGTCTAA

[1229] AATAGTGATGGGTCTAGGTCACGCTGTTTTGGCATCTGTTGTACTATACTGCGCCAAGTC

[1230] TATTGACCTTAGATCCAAAGCGTCAATTACATCCTTTTACATGTTAATCTGGAAACTTATG

[1231] TACGTCGAAAGCTTTCTAATGCCCTTAGCAAGGTAA

[1232] >SEQ ID NO:81

[1233] ATGATTTCCGTGCACGTAGGTAGTTTCCCAAATACATACACATTTATAACGGGTAGGAAT

[1234] CTTTGGCAAGGCCAAAATTGCACCAAGAATTTCTACGCTTCCTCTTACACTCCCAAGGC

[1235] CTCCCAGCAAAAAAGGAAAATCAAACAAGAATATAACTTCTTGAGATCTAGACAACCT

[1236] TCTCTTAAACATTTGTACAAAGGGATAGGTGGCGGTAGTTCATACCAGGAATGCAATAG

[1237] AAAATACGTTGTTAAGACGGCGCATAAACCATCCTTTGAGAGTGATCCCAGATCCATCG

[1238] ACAGGAAAAACATCCTTGAGTCTATAAAAAATTTTTTACATGCTTTTTATATGTTCTGTA

[1239] CTCCATACGCTTCAATTGTCGAGAAATTGTCTGACATTAGCCCCTTATTCTTTACTGGCG

[1240] TTATGCAAGCAATGGTTCCTCATATCTTCATCAATATTTTTGTTGCGGGCATAAATCAATT

[1241] AACAGACGCTGAAATTGACAAAATTAATAAGCCACATCTGCCCCTTGCATCTGGAAAG

[1242] ATGTCCTATACCACTGGAGTGATTATCGTAGCTTCCTCCCTAATTCTGGGATTATGGTTCG

[1243] GTTGGATTATTGGATCTTGGCCATGCATTCTTACTTTAATCTCTTTCGCCGTTATTTGGTC

[1244] TGCCTATAGCGTGAACGTTCCATTACTTAGGTGGAAGAGAAACCCAGTTTTAGCAGCGA

[1245] TGTGTTGCGTGGTCACTCTGTCAGTTGCAATTCCAATTGGTTATTTTTTCCATATGCAAA

[1246] CATTCGTATTTAAGCGTCAAGCATTTTTTTCTAGGCCATTGATCTTCGCGGTGGCGTCTC

[1247] TGAATCTTTTCTCTCTGGTGCTGGCACTGTTCAAAGATCTTCCCGATGTGGAAGGTGAC

[1248] AGGGAATTCGGTATTCAAACCTTCGCTATGAGATTGGGTCAGAAGAGAGTTTTCTGGAT

[1249] CTGCATTTTTTTGTTAGAAATGGCTTACGGTATTGCACTTTTAATGGGTGCGACATCTCC

[1250] CTATCTGTGGTCCAAGATCGTCACAGTTTTAGGGCATGCAATCTTGGCTCTGGTCGTCTT

[1251] ATACCACGCGAAAAAATCTATCGACTTGAGATCTAAAGCTACGGCCATCAGTTTCTATA

[1252] AGTTCATCTGGAAGTTGCTTTTCATGGAATTGTTTTTAATGCCTTTCGTTCGTTAA

[1253] >SEQ ID NO:82

[1254] ATGGCTATCCCATTATTGAAAATCGAAAAAATTTCCCTAGCTGAAAGAACTGTACCTTTG

[1255] TTGTACGACTTATTGGAATTCTTGTGTTGGCAAAAGATTTCCTCTACTGAGGCAAGTTTT

[1256] ATCAAAGATATGAAGACTGAAATGAGGAATAAGCTTATCAGAAGATTGAAAAAGTTACT

[1257] GAGTAGGTCCGTTTGCAGGACAAAACCTAGTAGTCCGAATCACCAAACACATGTGGAT

[1258] GTAAGGGAAGAATATGCTAATGCTTTTCGTACAGAGTCTTATATTGAATTTTGGACTAGG

[1259] GTGTTGGCGTATTCTAATAAGGGAGAATACACGTCTTGTCTGTCAAGAGAATTTACAAC

[1260] CAGTGCCAGGCTACCATCTTACCGTTTGTTTGCCGAACATCTATTGGATCCAGATCAACC

[1261] AACAATCACTCGTGTCTTGGCACAAATTCAACTGAGGCCTGAAATTCATTCTCTTTTGA

[1262] GCGATTATTTTGCACATACCGCGAATGCGTCTATGCTATGCAGCCACATCTTAAAAGATA

[1263] TTAATAGGGTAAGGGTCAAATACACTAGTCTGAAAACAATATTGCAATGCGTCCCAACC

[1264] AAGCAAATTTCTTCATCCATGGTTATGACACACTTAACAGAGTTCTCAAACAGCTTGAA

[1265] TCCCTTCGCCACATCCAGCCCCGCCCAGTGTAGAGTTAGAGCATCTGAATTGCAAAAA

[1266] AGATTAGAGAGTTCTCGTGACAAAGCTAGAGCTAAGTTGCATTTAATCGCAAGGTTAAA

[1267] GAGATGCTCAGCTTGTTTAGTTGTAATTGTTACAACGTCATTGGTAGTTTTCGTTGTAAC

[1268] TCATGGGTTTGCTTTGTTGGTCGCGGTTAGTGGTTTAGCCTCCATGAACTTGGTTTCTAA

[1269] AAGAAAATTGGCTAAAGTTACCGCACAATTGGACGCCGCAGCCAAGGGGACATATATA

[1270] CTTAATAAGGATCTGGAAACCACATCCAGGCTGGTAGCTCGTCTTAACGACGAATTAGA

[1271] ACATATGCGTACGACTATAAAGTTCTGGCTGGAAAAGAAAGACGATGGAATCTTCCAA

[1272] GCTGACGGTGAAGTTGTGAGACTCTTAAAAAAAATCAGTGTGGTTTTTCCGATCAATT

[1273] GGACGAGCTGGAAGAGCATCTGTATCTTTGTTTTATGACGATTAAGGGCAAGGGATC

[1274] TAGTTCTAAATCAGATTACCAATTCTGCTTAA

[1275] >SEQ ID NO:83

[1276] ATGCGTAACCATTGGGCTTTAGTGGGGGGTAACTTATGGCAAGGGCAAAATTGTACCAA

[1277] AAACTGCTACGCTTCAAGCTACACTCCCAAGGCCTCTCAGCAAAAACGTAAAATCAAA

[1278] AAAGAATATAACTTTCTGAGGTCACAACAACCATCCTTAAAACATCTGTATAAGGGCAT

[1279] TGAAGGTGGTTCTTCCTATCAAGAATGTAATAGGAAGTACGTCGTTAAGACAACCCATG

[1280] AGCCTAGTTTCGAGTCTGATCCCAGGAGTATCGATAGGAAGAATATTTTAGAATCAATC

[1281] AAGAATTTCCTTCACGCATTTTACTTGTTTTGCACACCCTACTCAGTGATTGGGACCGCC

[1282] CTAGGTATCATTTCTGTGTCATTACTTGCAGTTGAGAAATTAAGCGATATATCACCTCTTT

[1283] TCTTCACTGGTGTGATACAAGCAGTGGTACCCACATATGTTCATTAACATTTTTATCACTG

[1284] GGATCAATCAGCTTTCAGACGCTGAAATTGATAAGATCAATAAGCCGCACTTACCATTA

[1285] GCGAGTGGTAAAATGAGCTACACTACTGGCGTGATCATTGTAGCTAGCTCATTGATAAT

[1286] GGGCCTGTGGCTAGGATGGATAATAGGCTCATGGCCTTGTATCTTGACCTTAATTTCATT

[1287] CGCCGTTATATGGGGTGCATACTCAGTTAATGTCCCTCTGCCTTCGTTGGAAAAGAAATCC

[1288] AGTCTTAGCGGCTATGTGTAACGTGGTGTCATTGTCAGTAGCGTTCCCAATTGGTTATTT

[1289] TTTTCATATGCAGACTTTTGTTTTCAAGAGGTCAGCATTCTTTTCTAGGCCCCTAGTTTT

[1290] CGCTATTGCATTTCTTAATTTGTTCAGTCAGGTTATTGCTCTAAGTAAAAGACTTGCCAGA

[1291] CATAGAAGGGGACAGAGAATTTGGAATTCAAACTTTCGCCGCTAGGTTGGGTCAGAG

[1292] AGGGTATTCTGGATCTGTATATTTCTTTTGGAAATGACCTACGGCGTAGCATTGCTTATG

[1293] GGTGCTACCAGTCCTTATCTTTGGAGCAAAATAGTTACTGTATTAGGTCATGCCATCCTT

[1294] GCGTTGGTAGTTCTTTATCGTGCTAATAAGTCTATAGACTTGAGGTCTAAAGCTAGTGCC

[1295] ACCAGCTTTATAAGTTCATTTGGAAATTATTATACATGGAACTATTCCTTATGCCATTCG

[1296] TAAGATAA

[1297] >SEQ ID NO:84

[1298] ATGGATTCAACTGCTGCTTCCAAAATCTTAGTTATTGGAGGTACTGGTTACATGGGGCG

[1299] TTTTCTAGTGGAAGCCTCTGCTAAAGCTGGGCATCCGACTTTCGCCTTGGTAAGACCA

[1300] GCACTGTCACTAATCCAGATAAATCATCTATCATTCACTCATTCAATACATTGGGTGTGA

[1301] ACTTGCTATTGGGCGATATCAACGATCATCAGTCTCTGGTGAAGGCTATAAAAACAAGCT

[1302] GATGTTGTAATATCAACAAGTAAACCACCAATATATCTCTGATCAATACAAAATAATAAGT

[1303] GCGATAAAGGAAGCAGGCAACATTAAGCGTTTCTTCCCCTCCAGAATTTGGTAATGATGT

[1304] TGATCGTACCCACGGAGTTGATGGAGCGAAAGCGTTATTTGATATAAAGGCTAAATTTA

[1305] GGAGAACGATAGAAGCGGAAGGAATCCCACATACATATGTCGTTGCGAATTTCCTAACC

[1306] CAACATTTTCTGCCAACTAGGTCTAGATTATTAGCTATCGCCGCACCTTTGGACAAGGTA

[1307] GTGATTTTAGGTGATGGCAATACGAAGGCTACCTTCAACACTGAGGAAGGTGTGGCTA

[1308] CTTTCACAATAAGAGCTGTCGACGATCCTAGAACCTTGAACAAAATTCTGTACATCAGG

[1309] CCACCTGCTAATACATTGAGTTATAATGATCTAGTATCATTATGGGAAAAAAAAACTGGT

[1310] AATACTTTAGAAAGAGTTTACGTACCGGAAGAACAAGTTCTAAGACTGATTCAAGAAA

[1311] GCTCTTACCCATTGAATATGGCCCTGAGTATCTGTCATGCTGCATACGTTAAGGGAGATC

[1312] ACACAAACTATGAGATAGAACCGTCATTTGGGGTTGAAGCATTGGAACTATACCCTGAT

[1313] GTTAAATTCACAACTGTGGATGACTATTTGGAGCAAAATAGGGATTGCACTCCGTTCTA

[1314] CCTTAATCAATTAATCCCAATCACGAATGGTACTAAATTTTAA

[1315] >SEQ ID NO:85

[1316] ATGTCATTGCCACATAGTTTTTTTTTTTTTTTCCCTAACATTTCAAATCATGTTAT

[1317] TACTAGAACCCATGGTGTATTCTCTGAACAGCCCAACATTATGTTACCCACAGATCATCA

[1318] TCACCATCACCAAGGAACAGAAAACTGACCCATCCATTTTTTACCATGACATCA

[1319] GAAATACAAGGATCCTACGATAGTTCAAATTATCGATACCCCTAAAAATGTACCGAATG

[1320] GTTTCGGTAGTACATTCGTTATGGATGATGCTATGACAGAAGGTCCAGAACTTTCTTCTA

[1321] AGCACATAGGTAGAGCACAGGGTTTGTTTGGGTTGGCATCCCTTACAAGACTTGGGCAT

[1322] GTTTATGCTGACAAACTTTGTTTTTACAGAAGGAAATTACGCTGGATCCACTCTTTCTAT

[1323] GTTGGGGAGAAATCCTATTTCTGAACAAAATAGAGAAATGCCTATTGTCGGGGGTACTG

[1324] GAGTATTTAGATTTGCCAGGGGATATGCGATAGCCAACTCTGTTAACAGTATCAGCCACCC

[1325] CAGAACACTTTGTCGTTGAATACAATATTACCGTGAGCCATCCATAA

[1326] >SEQ ID NO:86

[1327] ATGGCTGAGACAACTACATCAAAATCTAAGATCCTTTTTATAGGTGGGACAGGCTATATT

[1328] GGTAAGTTTATAGTAGAGGCATCAGTCAATGCCGGTCACCCAACCTTTGTGCTGATCCG

[1329] TGATTCAACTTTATCTAATCCCGCTAAATCTCCTATTATAGATAAATTTAAGTCATTGGCT

[1330] GTCAACTTGGTTTTCGGTGATTTATATGATCATCAGAGCCTAGTCAAAGCAATCAAACA

[1331] AGTGGACGTAGTAATATCCACCGTGGGTCATTTGCAGCTTGGAGATCAGGATAAGATAA

[1332] TAAGTGCAATTAAGGAATCAGGCAACGTGAAAAGATTTTTTCCTAGTGAATTTGGAAAC

[1333] GATGTGGATCGTACACATGCTGTGGAACCAGCAAAATCTGCGTTCGCCACTAAGGCCA

[1334] AAATCAGGAGGACAATAGAAGCTGAAGGAATACCATACACCTACGTTTCTAGTAACTTC

[1335] TTCGCTGGTTATTTTTTACCAACTTTATCTCAACCGGGCGCAACAGCTGCTCCTAGAGAT

[1336] AAAGTAATTATACTAGGGGATGGTAATCCAAAAGCTGTGGCAAACAAAGAGGAAGACA

[1337] TCGCAACATATACGATCAAGGCTGTGGACGATCCAAGAACATTGAATAAGATTTTATAC

[1338] ATACGTCCACCCGCCAACACATTGTCATTTAACGAGTTAGTGTCTTTGTGGGAAAAAAA

[1339] AATAGGAAAAACCTTAGGTAGAGTGTATGTCCCTGAGGAACAGTTGCTAAAGCAAATT

[1340] CAAGAGTCCTCTCCTCCAATTAATGTGATTCTGTCCATAGGCCACTCTGTGTATGTCAAA

[1341] GGGGATCACACAAACTTTGAAATAGAACCAGCTTTTGGAGTTGAGGCTTCAGCTCTATA

[1342] TCCTGACGTTAAATATACTACCGTTGATGAGTACCTTAATCAATTCGTTTAA

[1343] >SEQ ID NO:87

[1344] ATGGCAGCTGCAGAGACAAAGATATTATTTATTGGGGGTACCGGCTATATTGGTAAATTT

[1345] ATAGTTGAAGCAAGTATAAAGGCTGGCCATCCGACCTATTTATTGGTCAGAGAAACCAG

[1346] TTATCAGATCCAGCCAAGTCACCAATTATCCATAAGTTTAAGGCAAGTGGTGTAAAAA

[1347] TCTTATTAGGTGACCTATATGATCACCAGAGCCTGGTTGAGGCTATCAAGCAAGTGGAC

[1348] GTGGTGATCAGCACAGTCGGCCATTGGCAATTACCAGATCAAGATAAATTGTCTCAGC

[1349] AATTAAGGAAGCGGGTAACGTTAAAAGATTCTTCCCTAGTGAATTCGGCAATGATGTTG

[1350] ATAGAACGCATGCTGTTGAGCCTATCAAAACCACGTACCAAGTGAAAGCCAAATTACG

[1351] TAGAGCGATAGAGGCCGAAGGCATACCACATACTATCGTTAGCGCCAATTTTTTTGCGG

[1352] GCTATTTCTTGCCTTCTCTAAGTCAACCAGGTGCCACAAGTCCTCCTAGAGATAAGTTG

[1353] GTGATACTTGGTGATGGCAACCCAAAGTGTGTGTTCAATAGTGAAGTGGATGTCGGTAC

[1354] TTATACGATAAAAGCTGTCGATGATCCAAGGACTCTGAATAAGATCGTCTATATTAGACC

[1355] GCAAGCGAATACCCTATCATTCAACGAACTTGTGTCCCTTTGGGAAAAAAAGATAGGC

[1356] AAAACTTTAGAGAGAGTGCATGTCCCTGAAGATCAAGTATTGCAGCAGATCCAAAAGA

[1357] CCTCCGATGTTGTTTTGTCCATTTGCCACTCTGTCTATGTTAAAGGGGACCAAACTAACT

[1358] TCGAAATTGAGCCCTCATTTGGTGTAGAAGCTTCTGCATTATACCCTGACGTCAAATATA

[1359] CTACCGTGGATGAATTTTTGAATCAGTTTGTTTAA

[1360] >SEQ ID NO:88

[1361] ATGGATTCTACTGCAGCATCTAAAATATTGGTTATTGGTGGCACAGGATATATGGGCAGA

[1362] TTCTTGGTGGAAGCAAGTGCGAAGGCTGGTCATCCAACTTTCGCTTTGGTTAGGCACA

[1363] GCACCGTCACCAATCCCGATAAGTCTAGCATTATTCATTCTTTTAACACCCTTGGAGTAA

[1364] ACTTGTTATTAGGTGATATTAATGATCATCAGAGTCTAGTCAAGGCTATTAAGCAAGCTG

[1365] ACGTTGTAATCAGTACGGTGAATCACCAATATATTTCCGACCAGTATAAAATTATTAGTG

[1366] CGATCAAAGAGGCAGGCAATATAAAGAGGTTCTTCCCGTCTGAATTTGGAAATGACGT

[1367] CGTTAGAACCCACGGTGTTGACGGGGCTAAAGCCCTATTCGACATCAAGGCCAAGTTC

[1368] CGTAGAACGATAGAAGCTGAGGGCATTCCTCATACCTACGTAGTTGCAAACTTCCTAAC

[1369] CCAGCATTTTTTACCCACCAGATCTAGATTATTGGCTATCGCAGCTCCATTAGATAAAGT

[1370] AGTTATATTGGGAGACGGTAATACTAAAGCTACGTTCAACACAGAAGAGGGTGTAGCTA

[1371] CATTTACAATAAGAGCAGTTGACGATCCAAGAACTCTTAACAAAATCTTATACATAAGA

[1372] CCACCTGCTAATACTTTATCTTATAATGATCTAGTGTCCCTATGGGAGAAAAAAACCGGC

[1373] AATACGCTGGAACGTGTATATGTGCCAGAGGAACAGGTTTTAAGATTGATCCAAGAGTC

[1374] ATCATACCCGTTGAATATGGCTCTAAGTATTTGCCATGCTGCCTATGTTAAGGGCGATCAT

[1375] ACAAATTATGAAATAGAACCAAGCTTTGGTGTTGAGGCTCTGGAATTATACCCTGATGT

[1376] AAAATTCACCACTGTAGATGACTACTTGGAACAAAATAGAGATTGCACCCCATTCTACC

[1377] TAAATCAACTGATTCCCATTACTAATGGTACCAAATTCTAA

[1378] >SEQ ID NO:89

[1379] ATGGGAAAATCCAAAGTTCTTGTTGTAGGTGGTACAGGTTATATAGGTAGGAGGATTGT

[1380] CAAAGCCTCTCTAGCGGAAGGGCATGAGACCTATGTACTTCAAAGAGCTGAATTGGGC

[1381] TTTCAAATTGAAAAGCTGCAAATGTTATTGAGCTTTAAAAGACAAGGTGCGCATCTAGT

[1382] TCAAGCGAGTTTTTCCGATCACAACTCTCTGGTTGACGCAGTTAAAAAAGTTGACGTT

[1383] GTCATTAGTGCTATCTCTGGAGTACATATTAGAACACACTGCATATCACTTCAACTGAAA

[1384] CTAATCGATGCAATCAAGCAAGCTGGAAACATCAAAAGATTCTTACCCAGCGAATTTGG

[1385] ACTTGATCCAGCCAGAATGGGACACGCGTTAGAACCAGGCAGAGTCACATTCGATGAT

[1386] AAAATGGCTATAAGGAAAGCCATTGAGGAAGCGAATATCCCTTTCACTTACATATCTGC

[1387] CAATTTATTTGCTGGTTACTTCGCTGGGAGCCTATCCCAAATGGGCTCTTTTGTGCCACC

[1388] AAGAGACAAAGTACATTTGTTTGGTGATGGCAAGCTAAAGGCAGTTTTTTGAATGAA

[1389] GACGATGTTGCCACGTATACTATAAAGTCTATCGATCAAGAACCTTAAATAAGACA

[1390] CTATATCTGAGGCCCCCAGAAAATATTCTAAGTCAAGCTGAGCTTATAGGGATATGGGA

[1391] GAAGTTAATCGGAAAGGAATTGGAAAAGACCTACATTACACCGGAGGGTTTCTTGACA

[1392] AAGCTAAAAGGGCTAGATTACAAATTGCAAGTTGGTATCGGTCACTTTTACCATATCTTT

[1393] TACGAAGGATGTTTGACTAATTTTGAAATCGGCGAAGATGGTGAGGAAGCTTCTAAGCT

[1394] ATACCCTGAAGTTAACTACACAAGAATGGATGAGTACCTAAAGATTTACGTTTAA

[1395] >SEQ ID NO:90

[1396] ATGGCTGAAAAGAGTAAAATCCTTATAATAGGTGGAACAGGGTATATCGGAAAACATAT

[1397] CGTTGAAGCATCTGCAAAAGCAGGGCACCCAACCTTTGCATTAGTAAGAGAGTCCACC

[1398] TTATCCAATCCGGCGAAGGCTAATTTGTTGGATAAGTTTAAGACTTTAGGAGTTAACTTA

[1399] GTCCCTGGTGATTTATACGATCATGAAAATTTAGTGAAAGTCATAAAACAAGTGGACGT

[1400] CGTAATCTCTACGGTTGGCCACTTACAACTTGCCGATCAAGTGAAGATTATTGCTGCTAT

[1401] AAAGGAAGCCGGCAATGTTAAGAGATTTTTCCCATCTGAATTCGGTAACGATGTAGACA

[1402] GAGTCCATGCCGTCGAACCTGCCAAATCAGCTTTTGCAATTAAGGTGCAAATCAGACG

[1403] TACCGTTGAAGCAGAAGGCATCCCTTATACATACGTTTCCTCTAATTGTTTTGCTGGTTA

[1404] TTTTTTACCAACTCTGGCACAACCAGGGGCAACTGCGCCACCCCCACCGAGAGATAAA

[1405] GTTATTATATTAGGAGACGGCAACCCAAAGGCCGTGTTCAATAAAGAGGACGACATCGC

[1406] TACTTACACAATAAGAGCTGTAGATGACCCTAGGACTCTTAACAAGATATTATACCTGAG

[1407] ACCACCCCATAACATCTACTCTTTTAATGAGCTGGTAGCTTTGTGGGAAAAGAAAATCG

[1408] GCAAGACCTTAGAGAAGACTTATGTGCCGGAAGAAAAGTTGCTAAAAGATATTCAGGA

[1409] AGCCCCTTTACCAATTAATGTTGTCTTAGCTATCAACCATTCAGTCTTCGTGAAAGGCGA

[1410] CCATACCAATTTCGAAATCGAATCTTCTTTCGGAGCAGAAGCTTCTGAACTTTACCCTG

[1411] ATGTGAAGTACACTACAGTTGACGAATTTTTGGACCAATTTGTTTAA

[1412] >SEQ ID NO:91

[1413] ATGGCTAAATCCACTGCTTCTGCTACCTTCTTTACCTCCCTAATACTGCTTTTCCTTTTGT

[1414] TATCAGTAGTCACTGCTACATATTACCAATCCATGTCTCCCACAATGTTGGGTTTCCAAG

[1415] AGGAAAAATTTACTCATTTGCACTTCTACTTTCATGATGTTGTAACTGGGCCCAAACCC

[1416] AGTATGGTAATCGTAGCAGAACCAAATGGCAAAGCCAAGAACTCTTTACCCGTTTGGAA

[1417] CTGTTGTGGCTATGGATGATCCATTGACTGTGGGTCCTGAATCAGACTCAAAATTAGTT

[1418] GGTAAAGCCCAAGGTATTTACACTTCAATTTCCCAAGAGGAAATGGGATTGATGATGGT

[1419] TATGACAATGGCGTTCTCTGATGGTGAATTTAACGGTTCCACTTTATCCATTTTGGCCAG

[1420] AAACATGATTATGAGTGAACCAGTAAGAGAAATGGCTATTGTAGGAGGGACTGGTGCC

[1421] TTCAGATTTGCAAGAGGATATGCTCAAGCAAAGTTTTATAGCGTAGACTTCACCAAAGG

[1422] TGATGCAATCGTAGAGTACGATATCTTCGTGTTTCATTACTAA

[1423] SEQ ID NO:92

[1424] ATGGGACTTCTTTCCTTAAATCGTAGTATTCAATTGTCATCCCTTTTGTTCCCGATTGCGG

[1425] TTCTTTTGTCACTTTCATGGTACTCCGCGAGCGCTGCAAATTCAACAAATAATACTTTCT

[1426] TGCATTGTCTAGTTAATCACTCTGATCCAAGTCACCCTATTACAAGCGCCATATTTACGC

[1427] CCAATAACAGTAGTTTCCCAAGCGTTTTGCAGGCCTACATCAGAAATTTAAGGTTCAAC

[1428] ACTACTACTACGAGGAAACCATTTCTGATTATAACTGCTTTGCACGTATCACACATTCAA

[1429] GCTGCTATTATTTGCGGCCAAAATCATAATCTTCAAATGAAGATTAGAAGCGGCGGTCA

[1430] CGATTATGAAGGTGTAAGTTATGTTGCAGAAGTTCCTTTCTTTATTTTAGACATGTTTAAT

[1431] TTGCGTAGTATTGAGATTGACATGGATACAGAAACAGCATGGGTTCAAAGCGGTGCAA

[1432] CGTTGGGCGAATTGTATTATAGAATTGCCGAAAAATCAAAACATCATGGGTTTCCGGCT

[1433] GGTGTTTGTCCTACTGTCGGAGTTGGAGGTCATATATCAGGAGGTGGCTACGGCAACAT

[1434] GATGAGAAAGTACGGTTTAAGCGTGGACAACGTTATTGACGCCCAGATAGTTGACGCT

[1435] AAAGGGAGACTGTTAGACAGAAAGTCTATGGGAGAAGATCTATTCTGGGCTATCAGAG

[1436] GGGGTGGTGGAGGTTCATTCGGTGTTGTATTGAGCTACAAAATCAAGCTAGTTAAGGTG

[1437] CCAGAGAAGGTTATAGTCTTCCAAGTTCAAAGAACGTTGGAACAAAATGCAACAGATA

[1438] TTGTATACAATTGGCAACATGTTGCACCGAACATTGATAACGATTTGTTCATTAGAGTCA

[1439] TCTTAGATGTGATAAATAATACATCAACTCAAAACGGTACTAAGACCATACGTGCAAATT

[1440] TCATTGCATTGTTTTTGGGCGACAGTAAGAGGCTTGTTAGTTTATTGAACGAATCCTTTC

[1441] CTCAATTGGGCCTGAAAGAATCAGACTGTATCGAGACGAGCTGGTTGCAAAGTGTGTT

[1442] ATTCTGGGACAACAAGGACATAGCCACACCAGTGGAGATTTTGCTAAACAGACAACCC

[1443] CAGTCTTTGAACTATCTTAAAAGAAAGTCAGATTATGTAAAGGAACCTATATCTAAAGA

[1444] GGGGCTAGAAGGTATTTGGAAAAAGATGATCGAATTGGTCGATGCAAAGTTGTACTTC

[1445] AATCCCTATGGAGGGAGAATGGCCGAAATTCCTTCAAGCGCAACTTCCTTTCCACATAG

[1446] GGCTGGCAATTTATGGAAGGTTCAATATCAAGCGAACTGGAATGAGGCTGGCGAGGAG

[1447] GTTGCTGATCATTATATCGGTTTGACCAGAGAACTTAGGAAGTACATGACGCCATTCGTC

[1448] AGTAAACCCCAGAGGTGCTTTCCTTAATTATAAAGACTTGGACTTAGGAATTAATCA

[1449] TAATGGTAAAAATTCTTACTTCGAGGGTAGGGTATACGGTGTCGAATATTTCAAAGATAA

[1450] CTTTGACAGACTAGTTAAGATAAAAACCAAAGTGGACCCTGGGAATTTCTTTAGAAATG

[1451] AACAATCAATACCAACTCTTCCTTATAGAAAGTCTTAA

[1452] SEQ ID NO:93

[1453] ATGATGCCACTTTCCTCATATTTTGCAGTTGTAGTCATAGCCTTCCTATTTGCTGTCGCAT

[1454] CTAGCACCGCTGATAACCACGAAAGTTTCTTCCAATGTCTTCAAAGCTATAGCCATAATT

[1455] CCACCAGTATTTCTAAAGTCGTTTATACTCAAACCAACTCATCATATTCCTCTATACTTAG

[1456] ATTCAGTACACATAACAGAAGATTTACAAGTAATGCAACACCAAAACCATTGGTGATAG

[1457] TAACGCCCTTACAGGTTTCAGAGATACAAGCCACTATCATTTGTTCTCAGAGGCATGGT

[1458] ATGCATATAAAGAACAAGGTCAGGAGGTCACGATTACGAAGGACTTAGCTACGTTTCTCA

[1459] TGTTGACCCATTCGTCGTGATTGATTTAATCAATTTACGTGAGATTCAAGTCGATGTGGA

[1460] AAATAGCACTGCTTGGGTTGAAGCTGGCGCAACTATTGGTGAGCTGTATTACAGTATAA

[1461] GCCAGAAATCTAGGACGTTAGGGTTTCCATCAGGCTTTTGTCCGACAGTAGGCGTTGGT

[1462] GGTCAAATCAGTGGAGGTGGTTACGGCAGCCTGCTGAGAAAATACGGTTTGGCTGTTG

[1463] ACCATGTCATAGACGCCAGAATTATAGATGTTAAAGGGAGATTGTTAGATAGAGAAGCT

[1464] ATGGGTGAAGATTTATTCTGGGCAATTAGAGGTGGTGGTGGTGCTAGTTTCGGCGTGAT

[1465] CACTGCTTGGAAGATAAAGCTTGTACCAGTTCCTCCCACTGTTACTGTATTTACAGTCA

[1466] GCAAAACATTGGAACAAAACGCTACAAAATTGATTCATAAATGGCAGCACGTTGCAAA

[1467] CAAATTGGACGAAGATTTGTTCATAAGAATCTCATTAGCTAGGGTTAACTCTAGCCAAA

[1468] CGAGAAAGCTTACAATTCAGGCGACCTTCGAGTCTCTTTTTCTTGGGGGTGTCGATAGA

[1469] TTGTTACCGCTAATGCAGGAAGTCTTCCCGGAATTGGGTCTAATTAGAGAGGGCTGCAC

[1470] TGAAATGAGTTGGGTCGAATCAGTTCTATACTTATACGAATTTACCAGTGGTCAACCAC

[1471] CTTTAGAAGTTTTATTGAATCGTACTACCGATTTCATGTTTATGAAGGGTAAATCCGATTA

[1472] CGTTAGGGATCCAATACCTGACAGTGGCTTAGAAGGTCTATGGCCGATGTTTTATGAAG

[1473] ATGAAGCACAAGACGCCGTTATGACTTTGCAACCATACGGGGGCAAGATGGATGAGAT

[1474] TCCAGAATCTGAGATTCCATTTCCACACAGAGCAGGTAATATCTATGCCGCAATGCACT

[1475] GGGTAATTTGGAGAGAAGAGGGCGATGAGGTTGCGCAAAGACATATTAATTGGATTCG

[1476] TAGACTTTATTCTTATATGGAACCATTTGTTAGTAAATCTCCAAGAGCAGCATACGTGAA

[1477] TGAACGTGATCTAGACATTGGCGTCAATAATATTAATGGTTACACCAGTTACGAGCAAG

[1478] CGTCAATATGGGGTTTGAAGTACTTCAAGAACAATTTTAATAGATTAGCAAAAGTTAAG

[1479] ACCATGGTTGACCCCTTGAATTTTTTCAGGAATGAACAGTCTATTCCATCACTTATGTAA

[1480] SEQ ID NO:94

[1481] ATGCATAATACTATTATCAACAAAATGACACCTTTATCAAGCTACTTTACCGTGGTCGCT

[1482] ATTGCTTTTTTATTTAGCATTGCTAGTTCTACCGCTGACAATCACGAATCTTTTTTCCAGT

[1483] GCTTGCAGAACTATTCCCATAACTCCACCTCTATTTCTAAAGTGGTCTATACCCAAACAA

[1484] ATAGCTCATATTCTAGCATTTTAAGATTCAGCACGCATAACAGGAGATTTACAAGCAATG

[1485] CTACCCCCAAGCCTCTAGTCATCGTGACACCCTTACAGGTATCAGAAATACAAGCGACA

[1486] ATCATATGTAGCCAAAGACATGGTATGCATATTCGTACCCGTAGTGGGGGGCATGACTAC

[1487] GAGGGCTTGTCCTACGTTTCTCATGTAGACCCATTTGTCGTTATAGATCTAATCAATCTG

[1488] CGTGAAATTCAGGTTGACGTGGAAAATAGTACTGCTTGGGTGGAAGCTGGCGCTACAA

[1489] TTGGCGAGTTATATTACTCTATCAGTCAAAAATCAAGGACACTTGGTTTTCCTGCCGGTG

[1490] TTTGTCCAACTGTGGGAGTAGGTGGACAAATAAGCGGCGGTGGTTATGGACCTTTGCTT

[1491] CGTAAATACGGTCTAGCGGCTGATAACGTAATAGACGCTAGAATAATCGACGTTAAAGG

[1492] AAGATTACTAGATTCAGAAGCTATGGGTGAAGATTTGTTTTGGGCCATAAGAGGTGGTG

[1493] GAGGTGCCTCTTTCGGTGTAATCACTGCCTGGAAAATCAAGCTGGTTCCAGTTCCACCA

[1494] ACAGTTACCGTATTTAGAGTGTCAAAGACTCTTGAACAGAACGCAACTAAATTAATACA

[1495] TAAATGGCAACATCTTTTGGCAAACAAGTTAGATGGTGATCTGATAATAACAATCAAGT

[1496] TGGCAAGGGTTAATAGTAGTCAAACCGGAAAATTAACGGTACAAGCGACGTTTGAGTG

[1497] TGTGTTCCTTGGTGGTGTGGATAGACTGCTGCCTTTAATGCAAGAGGTTTTTCCCGAAC

[1498] TTGGGTTGATACGTGAAGATTGTACAGAAATGAGTTGGGTTGAGAGTGTGTTATATAAT

[1499] TACGGGTTCACGAGCGGTCAACCACTTGAAGTCTTATTGAATAGGACTTCACAGGCAG

[1500] ATGTTCTTTTCTTCAAGGGTAAATCCGATTATGTACGTGATCCAATACCCGATTCTGGCC

[1501] TGGAAGGTTTATGGCCAATGTTTTATGAGGATGAGGCACAAGATGCCTTATTAACCTTA

[1502] ATACCCTATGGTGGCAAAATGGATGAGATCCCTGAATCAAAGATCCCATTTCCACACAG

[1503] AGCTGGAAATATCTACTCCATACAACATTGGTTGTATTGGCAGGAAGAGGGTGATGAAG

[1504] TTGCACAGAGACATATTAACTGGATTAGAAGGCTTTACAGTTATATGGAACCTTTTGTGA

[1505] GTAAATCTCCTAGGGCTGCTTACGTTAATGAAAGGGATCTGGATATTGGGGTTAATAACA

[1506] ATGGATACACGTCATACGAACAAGCTAGTATTTGGGGTTTAAAGTATTTCAAGAATAATT

[1507] TCAATAGACTAGCTACTGTTAAAACCAGAGTAGACCCTTTAAATTTCTTCAGAAACGAG

[1508] CAATCTATCCCTAGTTTGATGTAA

[1509] >SEQ ID NO:95

[1510] ATGGCCGCCAAACCTTTTCTACCCTTATTTTTTTACTTAGTATCGTAAGCGCATCTC

[1511] TGGCTGCACCAAAATACTACCGAGAGTCTGTACACAACCTTCTTACAGTGCTTGACTCAA

[1512] CATACAGATCCGAGTATCTCCAATCTAGTTTTCGCAAACTAATTCATCTTTTAGTACC

[1513] GTACTTCAGAATTACGTAAGAAATGCAAGGTTTAATACGTCCTCCACACCAAAGCCTCT

[1514] GATAATTGTAACCCCAGAGCAAGAACCTCATGTCCAAGGCACCGTATTATGCGCTAAGT

[1515] CCATTAACGTTCAACTAAAGATTAGGAGTGGAGGTCACGATTATGAAGGTATATCTTAC

[1516] ATTTCTAACGAACCCTTCATAATATTAGACCTTTTTAATTGAAAATTACTGTTGACA

[1517] TTAAGAACGAGGTCGCTATGGTACAAAGTGGAGCGACTCTAGGTGAAGTCTACTATAG

[1518] AATTTGGGAGAAATCAAAAGTACATGGCTTCCCTGCGGGAGTTTGTCCAACAGTAGGA

[1519] GTCGGAGGACACTTTAGTGGTGGTGGTTACGGCAACATGTTGCGTAAATTCGGCCTTTC

[1520] TGTTGACAATGTAATTGACGCTCAGATAGTAGACGTTAAGGGCAGAATCCTGAATAGAG

[1521] AGTCCATGGGCGAAGACCTTTTTTGGGCCATTAGAGGCGGTGGAGGTGCAAGCTTTGG

[1522] AGTGATATTGTCTTATACGGTTAAGTTAGTAAGGGTGCCAGAAACTGTCACCGTGTTCC

[1523] GTGTCGAAAAAACATTAGAACAGAATGCCACAGACCTGGTAGTTCAGTGGCAACAAGT

[1524] TGCACCCACAACTGATGACAGATTATTCATGAGATTATTGTTACAACCAGTTTCCTCTAA

[1525] GGTGGTTAAGGGTACTAAAACTGTTAGAGCATCCGTGGTGGCCCTATTTTTAGGTGTTG

[1526] CCGATGAAGTAGTGTCTCTGTTGGGTAAGGAATTTCCGTTACTTGGTCTGAAAAAGGA

[1527] AAATTGCACCGAAGTTTCATGGATCGATTCAGTTCTTTGGTGGGACGACGATGAATCCT

[1528] TGAAGAACGGAGCTAAGCCTGAAACATTGCTGAATAGAAATTTAAATTCCGCATCCTTT

[1529] TTGAAGAGAAAATCTGATTATGTGCAAAATGCTATATCAAGAGATGGTTTAGAATGGATT

[1530] TGGAAGAGAATGATTGAATTGGGTAAAACCGGTTTTGTGTTTAATCCTTATGGTGGCAA

[1531] AATGGGGGAAATTGCTTCCGATGCTACCCCTTTTCCTCATAGAGCTGGTAACCTATTCAA

[1532] GATCCAATACAGTGTCAACTGGGATGATCCATCCCAAGCAATGGCGTTGAACTTTACAA

[1533] ATCAAGCCAAGCGTTTGTATTCTTATATGACCCCATTTGTGTCCAAGAACCCTAGGAGC

[1534] GCCTTCTTAAATTACCGTGACCTAGACATCGGCGTAAATTCTTTTGGCAAAGATAGTTAC

[1535] GAAGAGGGGAAAGTATATGGCTCTAAATATTTCAATGACAACTTCGAAAGGTTGGTGA

[1536] AAGTGAAAACTGCAGTAGATCCGGAAAATTTCTTCCGTAATGAACAATCTATACCTGTG

[1537] TTACCGGCAGGTAAAGCTTAA

[1538] >SEQ ID NO:96

[1539] ATGGCTTTTACTTACTCTACGAAAAAATTAGCTTTATTATCAATCATTACCATTTTCATCA

[1540] GTAGTTTCCCTGAACCTAGCACATCCGTCGACTTGGAAAGCGGCTTTTTACAATGCTTT

[1541] TCCTCTGGTTTGGGAAACTCTAACTCTACAGCGGAACTGGTATTAACTAAGAATTCCTC

[1542] AAGCTATGCATCATTGTTGCAGTCTAGTATAAGAAATTTAAGATTCATGGGAACATCAGT

[1543] ACCAAAACCTACTTTGATTGTCACTCCAAGGGATTTGTTTCATATCCAGACAAGCATAA

[1544] AATGCTCTGTCAAACAAGGGCTTCAGATAAGAGTGAGATCCGGTGGACATGATTATGA

[1545] AGGATTGTCATATGTTTCTAATGATGACGTCCCATTCCTAATTATAGATCTTACGAATTTA

[1546] AGGTCTATTACTATCGACATCAAGGATGAAACAGCATGGGTACAATCAGGAGCTACATT

[1547] GGGTGAATTGTATTATGCGATAGCTAAAAAGAGTAACGTGCACGGTTTCCCTGCAGGCT

[1548] CATGTCCAACCGTTGGCGTCGGAGGTCACTTTTCTGGTGGTGGGTTCGGTACTATTTTC

[1549] CGTAAATATGGTCTAGCAGCTGATAACGTTATTGATGCTCAGATGGTGGATGTCAACGGT

[1550] AAAATTTTGAAGAACCGTACACTTATGGGTGAAGATTTGTTTTGGGCAATAAGAGGGG

[1551] GAGGTGGGTCATCATTCGGTGTTGTCACTGCTTGGAAAGTGAAATTAGTTCATGTACCG

[1552] CCCAAAGTGACCGTATTCAATATTCCGAAAACATTAGACCAAAATGCATCCACGCTGTT

[1553] TCATAAGTGGCAAATCGTTGCTGATAAATTACCAGGCGAGCTTTTCTTACATAGCGTAAT

[1554] GGGTGTGTCAAATGCTAACAGCATTTCTAGCAGTAGTGGTGGGGGTAGGACCGTTTTG

[1555] GTATCTTTCACTGGTTTGTATTTAGGCACTGTTGAAAATTTACTTCCGTTAATGCAAAAT

[1556] AATTTTGCGGAATTGGGGTTGCAACACAATAACTGCACCGAAATGTCATGGATACAATC

[1557] TGTTCTTTTCTTCGCTGGTTATGATCCAATCAACGATAGTCTTGAAGTTCTTTTGCAAAG

[1558] AAACCAGACATTTGGATCCTTTAAAGCAAAATCAGATTACGTTATGGAGCCTATACCAA

[1559] CATCTGGGTTAGAAGGCCTTTGGAATATGTTACTAGAGGAAAATTCATCCCCAACCTTA

[1560] ATTCTAACCCCTTACGGTGGCAGAATGTCTGAAATCTCAGAAAGCGAGACTCCATTCCC

[1561] ACACAGGAATGGCTCTATTTACGGAATCCAGTACCTGGTCTACTGGGATTCATCAGAAG

[1562] AGACTCCAAAGCATATTGCTTGGATGAGAAGAGTCTATTCATATATGGTCTTGTACGTTT

[1563] CCAAGTTTCCTAGGGCTGCCTACTTAAATTATCGTGACTTGGACATAGGTGTTAACAGA

[1564] GGTAATACATCATACGATTATGAAGAGGCTAAGTCCTGGGGCTTAAAATACTTTAAATGC

[1565] AACTTTGAGCGTTTAGCTAAAGTTAAGGCGGAAGTTGATCCATCAAACTTCTTTAGACA

[1566] TGAGCAATCAATCCCTCTTTTGTTCTAA

[1567] >SEQ ID NO:97

[1568] ATGGTTTCTCCCTCCTCTCATCTAGCAATCTTGATATTATTAATTTCCGTCTCATTTACCGC

[1569] ATCTTCTGCAAGTACTATCGAAGAAAGCTTCGTCCAATGCTTAAGTTTTTATTCTGATAA

[1570] AGCTGCTCCGTTTTACGCTACTATATACACCCCAAACAACGCTTCCTTCACCAAGATATT

[1571] GAACAGCTCTGCCCAGAACCTTAGATATTTGGTTCCTAGTGCCCCTAAACCAGAATTCA

[1572] TTTTTACACCATTGACTGATTCTCATGTTCAAGTCGCTGTGATCTGTTCCAAAAAATTAG

[1573] GTATACACCTTAGGGTCAGGTCTGGCGGGCATGACTATGAAGGTTTGAGCTATGTATCC

[1574] GAAATAGAGACGCCTTTTATAATAATTGACTTAGCTAAGTTGAGGGATATCAATGTTGAT

[1575] ATTGAGGATAACACAGCGTGGATCCAGGCAGGAGCCACCATAGGTGAAGTCTATTACA

[1576] GAATTTACGAAAAATCTTCCGTCCACGGTTTCCCTGCTGGATTGTGTACAAGTCTGGGT

[1577] GTTGGTGGGCACATTACAGGTGGTGCATACGGGTCTATGATGAGGAAGTATGGTTTAGG

[1578] TGCCGACAATGTCTTGGATGCACAAATCGTAGATGCTAATGGCAAAATCTTGGATAGAA

[1579] AGGCAATGGGAGAAGATTTATTTTGGGCTATTAGAGGTGGCGGGGGTGGGAGCTTTGG

[1580] TATTCTATTATGGTGGAAGATTAAGTTGGTACCAGTACCAGAAACAGTTACTGTTTTTAC

[1581] GGTCACCAAGTCACTTGAACAAGACGCTACAAGACTTGTACATCGTTGGCAGGAAGTA

[1582] GCTCCATACATAGACGAAGAACTGTTTATTAGGGTGATTATCCAACCCGCCACTGTTGG

[1583] TAACACAACCAAACGTACCATTACCACCAGTTACAATGCTTTGTTCCTAGGAGGAGCTG

[1584] ATAGGCTGCTACAAGTTATGAAAGAATCCTTTCCTGAGTTGGGTCTTACGAAAAAAGAT

[1585] TGCCTAGAAACCTCTTGGATTAAGTCTGTCTTGTATATTGCTGGTTTTCCGAATGACACT

[1586] CCGCCTGAGGTGTTGTTAAACGGCAAATCCACATTCAAAAATTACTTCAAAGCAAAATC

[1587] CGACTTCGTTAGAGAACCTATTCCCGAGACAGGTCTGCAAGGGCTATGGCAAAGATTA

[1588] CTAGAAGAGGATTCTCCCCTAATGATTTGGAATCCATATGGTGGCATGATGTCCAATTTT

[1589] AGCGAATCTGACATACCATTTCCTCACAGAAATGGTACCTTGTACAAGATACAATACCTA

[1590] ACTTTATGGCAAGACGGTGATAAGAATGCCTCAAAACATATAGATTGGACCAGGAAACT

[1591] ATATAACTACATGACACCATACGTCAGCAAATTCCCAAGAGAAGCTTATGTTAACTACA

[1592] GAGACCTAGACCTTGGTATGAATAAGAAAAATAGCACTTCTTATATCCAGGCAACAGCC

[1593] TGGGGCAATATGTACTTCAAGGACAATTTTAACAGGTTAGTTAAAATTAAGACCAAGGT

[1594] CGACCCAGAAAATGTTTTTAGGCATGAACAAAGCATTCCGCCCTTGCCAGTTTCAACC

[1595] ATGCAGTTAAAAGATAAGAAATGCAAGAACTGGGAGTAA

[1596] >SEQ ID NO:98

[1597] ATGCAGGCTTTACGTTCTTTTCTAGCATCTTTTTTAGTATTACTGAGTATTAGCTTGACAA

[1598] CTTCAACACCAATAGAAGAAACATTTAATCACTGCTTAACATTACATTCTCAACCACCA

[1599] AATCAGTTCCCTTCCACAATATATACCTCTAGAAACGACAGTTACACTTCAATCCTTAAC

[1600] TCAACTGCCCAAAATTTAAGATACTTGCTGCCCTCTGTACCCAAACCTGACTTTATATTT

[1601] ACGCCCTTCCATGACTCTCAAGTTCAAGCTGCAGTAATTTGCGCAAAAAAACTGGGTAT

[1602] TCATATGAGGGTTAGATCTGGGGGTCATGATTATGAAGGTTTGTCCTATGTGTCACTTAT

[1603] AGAAAAGCCATTCATGATCTTGGACTTGGTCAAATTGAGAGCTGTTAACGTGGATTTAC

[1604] CCCATAATACAGCTTGGATCCAAGCAGGAGCTACCATTGGTGAGGTCTATTACCGTATTT

[1605] CTGAAAAGTCAGCTGTACATGGTTTCCCCGCTGGTCTTTGCACAACACTGGGAATAGGT

[1606] GGACACATTACAGGAGGTGCCTACGGTAGCATGATGAGGAAATACGGATTGGGAGCGG

[1607] ATAACGTCCTAGATGCAAGAATCGTCGACGCAAATGGAAGAATACTGGATCGTGCTGC

[1608] AATGGGAGAAGATCTGTTTTGGGCAATTCGTGGTGGAGGAGGAGGTAGTTTCGGTGTG

[1609] ATTTTGTGGTGGAAAATTAAGTTGGTACCAGTACCAAAGACGGTAACTGTTTTTACCGT

[1610] CACCAAGTCTTTAGAACAAGGTGCTTCTAAGTTGTTACAACGTTGGCAAAAGGTCGCC

[1611] CCTAATATAGATGAAGATCTGTTTATAAGAGTTATAATTCAACCAGGTAACGGTAGCGTT

[1612] CCTGGACAAAGAACTGTGACCACAAGTTATAATGCATTATTCTTAGGTGGTGCTGGAAG

[1613] ACTATTACAAGTTATGAAGCATAGTTTTCCCGAGTTGGGTTTAACAAGAAAGGATTGTT

[1614] TGGAGACCTCTTGGATTAAGTCCGTTCTATATATAGCCGGGTATCCGAATGGTACACCAC

[1615] CTGAAGTGTTGTTACAGGGTAAGCCTACTTCAAAAGCATACTTTAAGGCAAAGTCAGAT

[1616] TTCGTAAGACAAGTCATCCCGGAAGCCTCTCTGAACGCTATTTGGAAGGTTTTCTTACA

[1617] AGATGATGGTCCACTGATGATTTGGAATCCCTATGGTGGCATGATGGGTCGTATTGCCGA

[1618] ATCTGCTACACCATTCCCTCACAGAAAAGGTACACTATATAAAATTCAGTATGTCACTGG

[1619] TTGGATTGATGGTGAAAAGTCCATGGCTAAACATATGAATTGGATGAGGAAATTCTATTA

[1620] TTGTATGGCCCCATATGCGTCTAAGTATCCCAGAGAGACATACGTTAACTATCGTGATCT

[1621] GGACATTGGAATGAACCAAAAAAATTGTACGTCCTTCAGCCAAGCACACAGTTGGGGA

[1622] TACAGATATTTCAAAGGTAACTTTAATAGACTAGTTAAGGTAAAGACCAAAGTTGATCC

[1623] GAGCAACTTTTTCAGACACGAACAAAGCATACCTCCACTAAGTACTGGTAAGAAAGGG

[1624] TAA

[1625] >SEQ ID NO:99

[1626] ATGGTATCCCCAATTTCTTTGCCCTGGCCTATATTAGTTTTGCTATTACTTTCCTCTTCATT

[1627] CTCATACCTTTGCCAGTTTCAGCTTCATTCAAGAATCATTCATTCAGTGTCTAAACTT

[1628] AAACAGCGATAGGACGTTTCCATTTCATACTACAATTTACACCCTAACGATGCATCTTT

[1629] TATTACAATTTTGGATTCTAGTGCACAGAATCTTAGATGTCTATTACCATCTGCCCCCAAG

[1630] CCAGAGTTTATTTTCACCCCTAGTAGAGATAGTCACGTTCAAGCGGCTGTCATATGTTCT

[1631] AAAAAACTGGGTATACATTTAAGAGTCAGAAGTGGTGGACATGATTATGAGGGTGTATC

[1632] TTATGTGTCAGAAATAGAGACGCCGTTTATGGTGGTGGACTTGGTAAAGCTTCGTGGCA

[1633] TTAACGTTGACATCAAGACTAACACGGCTTGGGTTCAAGCAGGTGCCACCATCGGTGA

[1634] GGTTTACTATAGAATCTATGAAAAAAGCTCTGTTTTAGGTTTTCCAGCAGGCTTGTGTAC

[1635] ATCCTTAGGTGTTGGTGGTCATATCACAGGTGGTGCATACGGAACTATGATGAGGAAAT

[1636] ACGGTCTTGGTGCTGATAACGTCCTTGATGCGCAGATTGTCGATGCAAATGGGAAAATT

[1637] TTAAATAGGGCTGCTATGGGTGAAGATCTATTTTGGGCAATCAGAGGAGGGGGTGGTGG

[1638] AAGTTTGGGGATTTTATTGTGGTGGAAAATCAAACTAGTTCCCGTCCCACCGACAGTTA

[1639] CTGTATTTACAGTGACCAAGAGTTTAGACAAGGCGCCACTAAATTGCTTCACCGTTGG

[1640] CAAGAGGTTGCCCCTTATATAGATGAAAATTTGTTCATTAGAGTCATAATTCAACCTGCA

[1641] TCCGGAGGTGGTAATAAGACCCAGAGAACAATCACCACTAGCTACAACGCTGTATTTCT

[1642] TGGCGAAGCTAGACGTTTGTTGCAGGTCATGAAAACCAGTTTTCCAGAACTGGGACTA

[1643] ACAATGAAGGATTGTCAAGAAACTAGTTGGATTAAGAGTGTACTTTATATTGCTGGCTT

[1644] TCCATCCGGCACACCCCCAGAAGTTTTGCTTAAAGGTAAACCAACGTTTAAAAAATTCTT

[1645] TCAAAGCGAAGAGTGACTTCGTTAGGGAACCCATCCCCGAAACTGGAATCGAAGGTTT

[1646] GTGGCAGAGACTGCTAATAGAGGATAGCCCATTAATGATCTGGAATCCTTATGGTGGTA

[1647] GAATGTCCCAGTTTAGTGAATCAGATACACCCTTCCCCCATAGAAACGGTACATTGTAC

[1648] AAAATTCAATATTTGTCATTGTGGCAAGATGGGGATAAAAAATGCCGCCAAGCATATCGA

[1649] CTGGATACGTAAACTGTATAACTACATGACGCCATATGTTTCTAATTTTCCGAGACAAGC

[1650] GTACGTAAACTACAGGGATTTAGACTTGGGTATGAACTCCAAAAACTCTACTTCATACA

[1651] TTCAGGCATCTGCTTGGGGTTATAGATACTTTAAGGACAACTTCAACAGGCTTGTTAAG

[1652] ATAAAAACTAGAGTCGATCCAGAGAATGTTTTCAGACACGAACAGTCTATTCCACCTTT

[1653] ACCAATATAA

[1654] >SEQ ID NO:100

[1655] ATGTTAGTTGACACAGGGTTAGGTCTAATTTCAGAATTACAAGCAAAGTTGGGTTGGGC

[1656] TGTTTTGTTACAAATTGTTCCGATAACGATTGTAGCTTATAACTTGTTGTGGTTCATATAC

[1657] GCCAGCTTCTTCTCTTCTTTGAGAAAGATTCCCGGTCCTTTCTTGGCTCGTATTTCTAGA

[1658] GTATGGGAAATGAAAAAGACGGCAACGGGCAATATTCACGAGATCATGATGGACTTAC

[1659] ATCGTAGACATGGAGCTATCGTTAGAATTGGCCCACGTAGATATGACTTTGACACTATGG

[1660] AAGCTTTAAAAATAATATATCGTATCGGTAATGCTTTGCCCAAAGCAGACTATTACAAAC

[1661] CCTTCGGCTTACCATCTTTTCCAAATTTGTTTGACGAGCAAAACCCTGCTAGACACTCA

[1662] GCTATCAAAAAACAAGTTGCCTCTCTATACACGATGACTGCCTTGTTGTCATACGAAGA

[1663] GGGAGTCGACGGTCAGACCGCAATTTTGAAGGAACAATTACAGAGATTCTGTGATCAG

[1664] AAGCAAGTAATTGACTTGCCTAGGTTTCTTCAGTACTATGCCTTCGATGTTATCGGTGTC

[1665] ATTACAGTGGGCAAGTCTATGGGTATGATGGAGAGTAATAGTGATACTAATGGTGCATGT

[1666] TCTGCTTTAGATGGTATGTGGCATTATGCCTCAATGATGGCTTATATACCAAATATGCATG

[1667] CTTGGTGGTTGAGATTAAGCTCACTTTTACCGATTGAAGTACCTATAAAGGGATTGACG

[1668] GAATACGTTGAGAGGAGAATCATCCAGTACCGTTTGAAGGCGGCAGAGTTCGGGGATG

[1669] ATGCTGCATTGAAGGGAGAAAATAACTTTCTAGCCAAACTACTATTAATGGAAAAAAAA

[1670] GGTACTGTCACACCAGTTGAAACACAGCAGGCTGTTGGATTGAATATCGGTGCCGGCT

[1671] CTGATACAACAGCTAACGCTCTATCCACCATTCTGTATTATTTATATACAAACCCCAGGA

[1672] CCTTACACACTCTGAGAGAGGAATTGGAGCGTTATGTTAAAGATGGTCCGATTTCTTTT

[1673] CAACAATCTCAATCAATGCCATACCTGCAAGCTGTGATCAAGGAAGCATTACGTTTACA

[1674] TCCAGGAGTCGGTACTCAACTTACAAGAGTTGTTCCCAAGGGAGGTTTAGTTATTGAA

[1675] GGCCAATTCTTCCCCGAAGGTACAGAGGTTGGTGTGAATGGATGGGCATTATACCATAA

[1676] CAAGGCAATTTTTGGAAATGATGCATCTATATTTAGGCCAGAAAGATGGTTAGAAGCTA

[1677] ACGAAAATATTAACATTGGTGGTTCCTTCGCTTTTGGAGCGGGTAGTAGATCTTGTATTG

[1678] GTAAAATATATCCATTTTGGAAATGTCAAAGGCTATACCGCAAATTGTTAGAAATTTCG

[1679] ATATAGAAATTAACCACGGAGATATGACTTGGAAAAATGAGTGTTGGTGGTTCGTAAAA

[1680] CCTGAGTACAAAGCCATGATAACCGAGAAGATGTTGCTTATCTAGAGATGAATCCTT

[1681] GGTTTAA

[1682] >SEQ ID NO:101

[1683] ATGGCAGGCAATGTAGAAAAATTATTACCAAAAAGTATCGAAGAAATGTCCATGGATGG

[1684] TTACGAACCTCCTTCAGAATATGTCGTAAAGGGTAATAATAGTTTCGGTTCAAAGGACA

[1685] GCAGTACACTTATTCCAATTCCTATTATTGACGTCAGTTTGCTTTCTTCAGAAGGTGAAC

[1686] TAGAAAAACTACGTTCAGCTCTAAGTTCTGCAGGTTGCTTCCAAGCAATTGGACATGG

[1687] GATGTCTACGAGTTACTTAGACAAAGTCAGAGAGGTGGCTAAACAATTTTTTGCTTTAC

[1688] CTGTTGAAGAAAAGCAGCGTTACGCCAGAGCAGTAAATGAAAGTGAAGGATACGGCA

[1689] ACGATAGGATTGTGTCTGAAAAACAGGTCCTAGACTGGAGTTACCGTCTGACCTTGAG

[1690] AGTTTTCCCTAAAGAGCAAAGACGTTTAAGCTTATGGCCCGAAAACCCAACTGACTTT

[1691] TCAGAGACCTTAGAGGAATTTTCAGCTAAAGTTAAATCTATGATGGACAGCCTTTTGAG

[1692] AAGTATGGCACGTAGCTTGAACTTAGAGGAAGGATCATTTCTAGATCAATTTGGTAAGC

[1693] AGAGTTCCCTGCAAGCTAGATTTAACTTTTACCCGAGGTGTAGCAGACCTGATTTTGTG

[1694] CTAGGAGTGAAACCACATACAGACAGGTCAGGTATTACCGTCTTGTTACAGGATAAAG

[1695] AGGTCGAGGGATTGCAAGTATTAGTAGATGACAAATGGGTCAATGTGCCTACAATTCCA

[1696] GATGCTCTAGTCGTGAATTTAGGTGATCAGATGCAGATCATGAGTAACGGCATATTCAA

[1697] ATCTCCAATGCACAGGGTCGTTACAAACACGGAAAAGTTGAGAATGAGTGTTGCCATG

[1698] TTCAACGAACCTGAACCTGAAAATGAAATTGGTCCAGTGAAGGATTTAATTAATGAGA

[1699] CAAGGCCACGTGTCTATAGGAACGTAAAAATTACGGCGAAATCAATTATAGATGCTAC

[1700] CAAGAGGGAAAAATCGCCTTAGAAACAGTCCAAATCGCAGATAATTGTGACCAAAAGT

[1701] AA

[1702] >SEQ ID NO:102

[1703] ATGGACGTCTCTTCAATCTTAGTTCCATCAGTGCAAGAATTAGCTAAAGAGGCTCTTAC

[1704] AAGAGTTCCTGAAAGATACGTGCTGAATCCTGATCAGGATCCATTACCTCTATCTAATAA

[1705] TTCAGCCCCATTGCCCGATCAAGTTCCTGTTATCGATTTAAGTAAATTACTATCTCAGGA

[1706] TGATTTAAAGGGGCCTGCAGAATTAGAAACTTAACAGTGCTTGTAAGGATTGGGGT

[1707] TTCTTCCAGTTGGTTAACCACGGTATAAGTACCTCTTGGTCGAAAATATGAAGACGGG

[1708] AGTCAAGACATTGTTGAACTATCAATGGACGAAAAAAAGCAATTATGGCAGAGAGAA

[1709] GGCGACTTGGAAGGCTTTGGGCAAGCATTCATTGTGTCAGAAGAGCAAAAACTGGAAT

[1710] GGGCTGATGCATTTTTTATGATTACACTACCCCCACACTTGAGGAAACCACATCTATTCA

[1711] ATCAGATCCCCCCAATCATTCCGTGAAAATCTTAGAGATTTATTCAGCGGGAATTAAAAC

[1712] CTGGCCATTAAGATTATAGAGCTAATGGCCAACGCTCTGGCTATAGATCCTAAAGAAATG

[1713] ACCGAAGTCTTCAAAGTCGGTATCCAAACAATACGTGTCAATTACTACCCACCTTGCCC

[1714] CCAACCTGAACGTGTTATTGGTCTTAAGTCTCATTCTGATTCAGGAGGATTGACGATATT

[1715] ATTGCAGGTTAACGACATAGAAGGTCTGCAAATTAGGAAAGATGGACTTTGGATTCCTG

[1716] TTCAACCTCTGCCGAATGCGTTCATTATAAATATTGGTGATATGTTAGAGATCATGACGA

[1717] ATGGCATATACCGTTCTATCGAACATAGGGCTACTGTAAACTCTGAAAAAGAGAGAATC

[1718] TCCTTGGCTACTTTCTATGGTCCGAACATGCAAGCCATTCTAGCGCCTGCTCCTTCTTTA

[1719] GTGACTCCAGAGAGACCAGAACAATTCAGAAGGATTTCAGTAGCTGACTACTTCAAAG

[1720] GTTATTTCGACCAAGAATTGTCTGGTAAATCCTACTTAGACGAAGTCAAGATTCCAAAG

[1721] GGCGAATAA

[1722] >SEQ ID NO:103

[1723] ATGGAACACTTCTATATGTCATTGCTTCTACTATTTGTTACCCTTGTATCTCTTTCACTGTT

[1724] TTTCTTGATTTTTTATCACAATAAGCACAATGAATAATAATAACAATTTGCCGCCTGGG

[1725] AAGATGGGCTATCCAGTAATAGGTGAATCTCTTGAATTCCTATCAATGGGCTGGAAAGG

[1726] CCACCCTGAAAAATTATTTTTGACCGTATGGTAAGATACTCATCTGAATTGATTAAGAC

[1727] TTCCATCTTAGGTGTTCCAACGGTCATATTTTGCGGACCAGCGTGTAACAAATTCCTATT

[1728] TTCTAATGAAAAACAAACTAGTTACCGCCTGGTGGCCAGACAGTGTGAACAAGATATTTC

[1729] CCACAACTTCTAATATAGTAAAGAGGAGTCCAAAAAAATGAGAAAATTATTGCCACAATTT

[1730] TTGAAACCAGAAGCTCTACAAAGATACGTCGGTATTATGGATACATTAGCTCAAAGGCA

[1731] TTTCGCAAGTTTGTGGGAAAAAACCCACGTGACCGTATACCCATTAGCTAAAAGA

[1732] TATACCTTCATGCTGGCTTGCAGATTATTTATGTCTGTGGAAGACGAAAATCACGTTGCT

[1733] AAGTTTAGAGAGCCGTTTCACTTACTTGCCAGCGGCATCATATCGTTCCAATTGACTTG

[1734] CCGTGGACCCCCTTTAACAGAGGTATAAAAGCTTCAAATTCATCAGAAAGGAATTGTT

[1735] AAAGATAATAAGGCAAAGGAAAGTTGATCTGGCACAAGGGGTTGCATCTCCTACCCAA

[1736] GATATTCTGTCACACATGCTGTTGACTTGTGATGATGAAAACGGCGAATTTATGACCGA

[1737] ACTTAATATCGCAGACAAGATTCTAGGTTTGCTAATTGGAGGTCATGACACCGCTTCAG

[1738] CTGCGTGTACATTTATAGTAAAGTATTTAGCTGAACTACCTCATATTTATGATAGAGTATA

[1739] TCAGGAACAGATGGAGATCGCAAATTCCAAGTCTCCTGGCGAATTACTAAATTGGGATG

[1740] ATATCAACAAGATGCGTTATAGTTGGAACGTTGCTTCTGAAGTCATGAGAGTGGCTCCC

[1741] CCTCTTCAAGGGGGGTTCAGAGAGGCAATTAATGACTTTGTTTTCAACGGCTTCAGCAT

[1742] CCCAAAAGGCTGGAAACTTTATTGGTCAGCTAACTCCACGCATAAAAACCCTGAGTATT

[1743] TTCCAGCTCCCGAAAAATTCGACCCAACTAGATTCGAAGGTAATGGACCCGCTGCATAT

[1744] ACTTTTGTTCCTTTTGGGGGTGGACCTAGAATGTGTCCAGGCAAAGAATACGCTCGTCT

[1745] AGAGATTTTAGTCTTTATGCATAACCTGGTCAAAAGATTTAAGTGGGAAATGTTGATTCC

[1746] TGAAGAAAAAATTGTTGTAGACCCTCTGCCAATGCCAGCCAATGATTTACCGATAAGAC

[1747] TTTACCCGCATAATACCTAA

[1748] >SEQ ID NO:104

[1749] ATGGACGCAACAACATTAACCGTGCCTTCAGTGCAAGAGATAGCTAAGGGCTCATTAA

[1750] CAACAGTACCGGAAAGATACGTTAGACCACCTCATGAAAGGCCCACCCACATTATTCGCT

[1751] ACCACTCCTTTGCCAGATCAGGTTCCGGTCATAGATCTAAGTAAGTTATTGTCTCAGGA

[1752] CGACTTAAAGGCCAGCAGAATTAGAGAAACTACATTCTGCGTGTAAAGATTGGGGA

[1753] TTCTTTCAACTGATTAATCATGGCGTAAGTATAAGTCTGCTAGAGAATGTTAAAAAGGGT

[1754] GCCCAAGAGTTCTTTAACTTGCCAATTGAAGAAAAAAAGAAAAAGTTCGGTCAGAAA

[1755] GAGGGAGACGTAGAAGGTTATGGACAAGCATTTGTCGTTTCTGAGGAGCAAAAACTTG

[1756] AATGGGCTGACCTGTTCTATTTGTTGACACTTCCTCCACATATGCGTAAACCCCATTTAT

[1757] TCCCCAACTTGCCTCTGCCTTTTAGGGACGACCTTGAGGCCTATACAGCAGAACTTAAG

[1758] AATCTAGCCATTCAGGTACTGGACCTGATGGCAAACGCTCTGAAAATGGATGCAAAAG

[1759] AGATGAGGGAATTATTCGGCGAGGGTGTTCAAAGTATGCGTATGAATTATTATCCTCCAT

[1760] GCCCTCAGCCGGAATTAGTTATGGGTCTGAATCCACATTCTGATGGTGGCGGTTTAACA

[1761] ATTCTACTTCAAGCGAATGAGGTTGAGGGACTTCAGATTAAGAAAGATGGCTTGTGGAT

[1762] CCCAGTAAAACCCCTTCCAAACGCTTTCATCATCAATCTTGGTGATATGCTTGAAATGAT

[1763] TACGAATGGGATTTATAGGTCAATCGAGCATCGTGCCACAGTTAACTTAGAAAGAAA

[1764] GATTAAGCATTGCAACTTTCTATAACCCAGGTGTAGAAATTACCGTCAGACCTGCACCG

[1765] TCTTTGGTGACACCCAAAACTCCTGCTGTCTTTAAAACTATTGGTGTTCTTGAATTTTAC

[1766] AGAGGATATTTCGCCAGAGAGCTTCAAGGTAAGTCATACTTGGACACCATGCGTATACA

[1767] ATCTGAAGATGAGAAGAGCTCTTAA

[1768] >SEQ ID NO:105

[1769] ATGGCAAACGGTGAAATCTCAAACTATACTGAATTAAACGTAAGACAAGGTGAACCTA

[1770] CTAGAGTTAAACCAGCTGAAGAAACCGAAAAAGGACTGTACTTCTTGAGCAATCTTGA

[1771] CCAAAATATTGCCGTCCCTGTACGTACAGTCTATTGTTTTAAATCTGCAAGCAGGGGTAA

[1772] TGAAGATGCAGCCCGTGTTATTAAGGATAGCTTGAGTAAGATTCTTGTTCCATATTACCC

[1773] CATGGCTGGAAAACTGATGATCAGTTCCGAGGGTAAGTTAATTGTTGATAACAATACGG

[1774] GAGAAGGTGCTGTTTTCGTGGAAGCGGAAGCTGATTGCGACATACAAGATATTGGTGA

[1775] CTTAACAAAGCCAGATCCCGATACATTAGGCAAATTAGTGTATAACACCCCAGGCGCCC

[1776] GTTCAATCTTAGAGATGCCCTTAATGACTGTCCAAGTTACTAAATTCAAATGTGGTGGAT

[1777] TCACATTGGGCCTAAATATGATTCATTGTATGAAGGACGGCCTTTGCGCCATGGAATTTG

[1778] TTAATGCTTGGTCAGAAACGGCCCGTGGTTTGGACACTAAAACCCCTCCCTTTCTAGAC

[1779] AGAACGATTCTTAAGGCACGTGATCCACCCAAGATAGAATTTCAACATCACGAATTTGA

[1780] TGAAATCGAGGATATCTCTAACACCGAAAATTTGTACGAGCAGGAAAAAATGTTTTACA

[1781] GATCTTTCTGTTTTGACCCCGAAAAACTGGAACAGCTAAAAAAAAAAGCTACGGAAGA

[1782] TGGTGTTTTGGAAAAATGCAGTACTTTTGAGGCCTTGTCAGCTTTCATTTGGAGAGCAA

[1783] GAACCGAAGCCTTGAAAATGCACCCAGATCAAAAGACCAAGTTATTATTTGCAGTCGA

[1784] CGGTAGATCTAGGTTTATACCGCCTGTGCCAGAAGGTTATTTTGGTAACGCAATTGTCTT

[1785] GACCAACTCTTTATGTAACGCATCCGAATTATTGGGCAACCCCCTGTCTTTTACTGTTGG

[1786] GTTAGTTAGAAAGGCTATTGACATGGTGAATGATTCTTATATGATCCGCTATAGATTA

[1787] CTTTGAACTTACTAGAGCCCGTCCCAGTCTTACAGCTACTTTGTTGATAACCACATGGA

[1788] CGAGATTGAGCTTTCATACCACGGATTTTGGTTGGGGTGAACCTGTTTGCAGTGGTCCA

[1789] GTAACCCTGCCAGAAAAGCAGGTAATTCTGTTTCTTTCTCAAGGACAAGAAAGGAAGT

[1790] CCATTAATGTCTTGTTGGGTCTACCAGCAAGTGCCATGGAATCATTCGAAGCACTAGTG

[1791] ATGCAAGTGTAA

[1792] >SEQ ID NO:106

[1793] ATGGATTCCCAGACAAAAAAAACTTGGCACCTCTCTGTTAGTACCTAGCGTGCACGAGC

[1794] TAGCAAAACAATTAGCCGAAGTACCAGAAAGATACGTTAGACCGAATCAAGACCC

[1795] AACACTGGTCCCAAAATACAATCAGTTCTACCTCAGGTCCCAGCTATTGACCTAAATAAGC

[1796] TGCTTTCTGAAGATGGTACCGAATTGGAGATGTTGGATCATGCTTGTAAAGAGTGGGGA

[1797] TTCTTTCAACTTATAAATCATGGGGTAAATCCAAGTCTGGTGGAGAATGTGAAAACGGG

[1798] TGTGCAAGAGTTTTTCTCCTTGCCTATGGAGGAAAAAAAAAAATTTTGGCAAAAACCT

[1799] GGTGAGCTGGAAGGATATGGTCAAAACTTTGTGGTTTCTGAGGAACAGAAATTGGAAT

[1800] GGGCCGACTTGTTTTACATCATTACCTTGCCATCCTACACACGTAATCATCATTTATTCTC

[1801] CTCCATCCCTAAGTCTTTTAGAAATGATTTAGAGAGTTATTGTTTGGAAATGGAGAATT

[1802] GTGCATAACTATCATTGAGCTTATGGCCAAAGCTTTGAAAATCAAACCAAATGAATTGT

[1803] TGGAATTGTTTGAAGATGTTAGTCAAAGTATGAGAATGAATTGTTACCCACCGTGCCCG

[1804] CAACCAGAACATGTGATGGGTTTGAACCCACATAGTGACGCAGGAGCTTTAACGATTC

[1805] TGTTGCAAGCCAACGAAATAGAAGGTCTGCAAATTAGAAAAAGATGGGATGTGGATTCC

[1806] AATCAAGCCATTGTCCAACGCGTTTGTTATCAACGTTGGTGATATTTTGGAGATTTTAAC

[1807] GAATGGAATATACAGATCTATAGAACACCGTGCAACCATTAACTTGGGACAGGAAAGA

[1808] ATATCTATTGCTACTTTTCATAGGCCCCAAATGCATAGAGTGGTTGGGCCAATGTCAACT

[1809] TTAATTACATCAGAGCGTCCGTCACTGTATAAACGTATTGGTGTTGCCGATTACTATAAG

[1810] GGATACTTTAGTAGAGAGCTACAGGGTAAGAGCTATATAGACGTCGTCCGTATCCAAAA

[1811] GGGTTAA

[1812] >SEQ ID NO:107

[1813] ATGGAAGAACTTAATAACCCCTCTGGTAGATATTGTTAGTCCCCATCCGTGCAAGAGTTA

[1814] GCAAAAGAAAGATTACCAACGTACCTCCAAAGTACATTCAACCTGAACACGAAAACT

[1815] TAGGAATTATTTCCGAGGATGAATGCGTCCTGGAAATACCAGTAATAGACATGCAAAGG

[1816] TTGATTTCCTTGGAATTCGGTTCCTCATCAGAATCTGAATTAGCCAAATTGCACCTAGCC

[1817] TGTAAGGAATGGGGTTTCTTCCACAGTGCATTGGCTCTGCAATCTTTTATTTATCTATTTT

[1818] TGATTAACCACGGGATTAGTTCCTCATTCCTAGAGAATGTTAAGCTTGAGATTCAGGAC

[1819] TTTTTCAACCTACCAATGTTAGAAAAAAAAATTTTGGCAGTCACCCCAACACATGG

[1820] AGGGATTCGGTCAAGCCTTTGTCCTAAGTGAAGATCAGAAGTTGGACTGGGCAGATAT

[1821] GTTTTACATGCTTACGCTACCTACACATCTTAGGATGCCTCATCTATTTCCTCAGCTACCT

[1822] CTACCATTTAGAGAGACCTTGGAACTGTACTCTCAGGAAATCAAAATCTTGGCTATGTC

[1823] AATTATAACTCACATTGAAAAGGCCCTGAAGATGCAGGAAATGGAAATAAGAGAACTT

[1824] TTCGAAGACGGTATTCAGATGATGAGAATGAACTATTATCCACCATGCCCACAGCCAGA

[1825] GAAAGTCATTGGTCTAACGAATCACTCCGATAGAGTGGGCCTTACTATTCTGTTGCAAG

[1826] TTAACGAGATCGAAGGGCTGCAAATTAAAAAAAACGGTATGTGGGTTAGAGTAAAACC

[1827] GTTGCCAAATGCATTCATCGTAAACATCGGCGACATTTTAGAGATAATGACCAATGGTAT

[1828] TTACCCCTCCATAGAACACAGAGCAACAGTCAGCAGTGAAAAAGAGAGACTATCTATT

[1829] GCAACCTTTCATTCTCCAAGACACGACGGTGTGGTAGGTCCAGCGGCAAGTCTAATCA

[1830] CAGAACAGACTCAGGCACAATTCAAAAGGATAGGAGTTGATGAGTACTTTAAGGGTGT

[1831] TTTTGCAAGAAAATTGGACGGCAAATCCTACATCTATGTTATGAGGATTAAGCATCACG

[1832] ATTAA

[1833] >SEQ ID NO:108

[1834] ATGGATGCTACTACCTTGACTGTGCCTTCAGTTCAAGAAATTGCTAAGGGTTCTTTGAC

[1835] CACAGTTCCAGAAAGATATGTCAGACCCACCACATGAAAGACCAACCACTTTGTTCGCA

[1836] ACTACACCATTGCCTGATCAAGTTCCAGTCATCGATTTGTCAAAGTTGCTGTCACAAGA

[1837] TGATTTGGAAGGTCCAGCAGAATTGGAGAAGTTGCATAGTGCTTGTAAAGATTGGGGT

[1838] TTCTTCCAACTGATTAACCACGGTGTTTCTATCTCTCGTTGGAGAATGTCAAAAAGGG

[1839] TGCACAAGAGTTCTTTAATTTGCCTATTGAAGAGAAGAAGAAATTCGGTCAGAAA

[1840] GAAGATGTTGAAGGTTATGGTCAAGCATTCGTTGTCTCTGAAGAACAGAAGTTGGAAT

[1841] GGGCAGATTTGTTCTTTCTGCTGACATTGCCACCTCACATGCGTAAACCACATCTGTTTC

[1842] CAAATTTGCCTTTGCCATTCAGAGATGATCTAGAGGCTTATACAGCTGAATTGAAGAATT

[1843] TGGCTATCCAAGTCTTGGACTTGATGGCTAATGCTTTAAAGGTCGATGCTAAAGAAATG

[1844] AGAGAATTGTTTGGTGAAGGTGTTCAATCTATGAGAAATGAATTATTATTCCACCATGTCCA

[1845] CAACCAGAATTGGTTATGGGTCTTAATCCACATTCTGATGGTGGTGGTTTGACCATCTTG

[1846] TTGCAAGCTAATGAAGTCGAAGGTTTACAGATTAAAAGAATGGTTTGTGGATTCCAGT

[1847] TAAGCCATTGCCAAATGCATTCATTGTCAATCTAGGTGATATGTTGGAAATGATCACTAA

[1848] TGGTATCTACAGATCTATCGAACATAGAGCTACTGTTAACTTAGAGAAAGAGAGATTATC

[1849] AATCGCTACATTCTACAATCCAGGTGTTGAAATCACCGTTAGACCAGCACCATCTCTTG

[1850] TTACACCTAAGACTCCAGCTGTATTCAAGACAATTGGTGTCTTGGAGTTCTATATAGAGGT

[1851] TATTTGGCAAGAGAATTGAGAGGTAAGTCTTATTTGGATACAATGCGTATTCAGTCTCAA

[1852] GATGAGAAGTCTAGTTAA

[1853] >SEQ ID NO:109

[1854] ATGGATGCTACTACCTTGACTGTTCCATCTGTTCAAGAAATCGCTAAAGGTAGCTTGAC

[1855] TACAGTTCCAGAAAGATACGTTAGGCCACCACATGAAAGACCAACTACACTGTTTGCT

[1856] ACTACTCCATTGCCAGATCAAGTCCAGTTATTGATCTAGGTAAGTTGCTGTCTCAAGAT

[1857] GATCTTAAGGGTCCAGCTGAATTGGAGAAATTGCATTCTGCTTGTAAAGATTGGGGTTT

[1858] CTTTCAGTTGATCAATCATGGTGTTTCTATCTCTCTGTTGGAGAACGTTAAGAAAGGCG

[1859] CTCAAGAATTTTTCAACTTGCCAATCGAAGAGAAGAAGAAGAAATTCGGTCAGAAAG

[1860] AAGGTGATGTTGAAGGTTATGGTCAAGCATTCGTTGTTTCTGAAGAACAGAAATTGGA

[1861] ATGGGCTGATTTGTTCTATTTGTTGACTTTGCCACCACACATGAGAAAGCCACATTTGTT

[1862] TCCAAATCTTCCACTACCATTCAGAGATGATTTGGAGGCTTACACTGCTGAATTGAAGA

[1863] ACTTGGCTATCCAAGTGTTGGATTTGATGGCAAATGCTTTGAAGATGGATGCCAAAGAA

[1864] ATGAGAGAATTGTTTGGTGAAGGTGTCCAATCTATGCGTATGAATTACTATCCACCATGC

[1865] CCTCAACCAGAGTTGGTTATGGGTTTGAATCCACATTCTGATGGTGGTGGTCTGACTAT

[1866] CTTGTTGCAAGCTAACGAAGTTGAAGGTCTGCAAATCAAGAAGTATGGTCTGTGGATT

[1867] CCAGTCAAACCATTGCCTAATGCTTTCATCATCAATTTGGGTGATATGTTGGAGATGATT

[1868] ACCAACGGTATCTACAGGTCTATTGAGCATAGAGCTACCGTTAATCTAGAGAAAGAAAG

[1869] ATTGTCTATTGCCACTTTCTACAATCCAGGTGTTGAGATTACCGTTAGACCAGCTCCATC

[1870] TTTGGTTACACCAAAGACTCCAGCTGTCTTTAAGACCATTGGTGTTCTTGAGTTCTATAG

[1871] AGGTTACTTCGCTAGAGAATTGAGAGGTAAGTCTTATCTGGACACTATGAGAATCCAGT

[1872] CTGAAGATGAGAAGTCATCTTAA

[1873] >SEQ ID NO:110

[1874] ATGGATGCTACCACTTTGACAGTTCCATCAGTTCAAGAAAATCGCTAAAGGTTCTCTTAC

[1875] AACCGTACCTGAAAGATATGTCAGACCACCACATGAAAGACCAACTACTTTGTTTGCTA

[1876] CTACTCCATTGCCAGATCAAGTTCCAGTTATCGATCTGTCCAAATTGTTGTCACAAGATG

[1877] ATCTAAAGGGACCAGCTGAATTGGAGAAGTTGCATTCTGCTTGTAAAGATTGGGGTTTC

[1878] TTCCAGTTGATCAATCATGGTGTTTCTATCTCTTTGTTGGAGAACGTTAAGAAGGGTGC

[1879] ACAAGAGTTTCTTCAACTTACCAATTGAAGAGAAGAAGAAGAAGTTTGGTCAGAAAGA

[1880] AGGCGATGTTGAAGGTTATGGTCAAGCATTTGTTGTCTCTGAAGAACAGAAGCTAGAA

[1881] TGGGCTGACTTATTCTTCTTGTTGACATTGCCAGCTCACATGAGAAAGCCACATTTGTTT

[1882] CCAAACTTGCCATTGCCATTTAGAGATGATTTAGAAGCTTACACAGCTGAACTAAAGAA

[1883] CTTGGCTATCCAAGTTCTAGATTTGATGGCTAATGCTTTGAAGATGGACGCTAAAGAAA

[1884] TGAGAGAATTGTTCGGTGAAGGCGTTCAATCAATGCGTATGAATTATTATCCACCTTGTC

[1885] CACAACCAGAACTTGTTATGGGTCTTAATCCACATTCTGATGGTGGTGGTTTGACCATCT

[1886] TGTTGCAAGCTAATGAAGTTGAAGGTTTGCAAATCAAGAAAGATGGTTTGTGGATTCC

[1887] AGTTAAACCACTACCAAATGCCTTTATCATCAATCTTGGTGATATGTTGGAGATGATCAC

[1888] CAACGGTATTTACAGATCAATCGAACATAGAGCTACAGTTAACCTAGAGAAAGAGAGAT

[1889] TATCTATCGCAACATTCTATAATCCAGGTGTTGAAATCACTGTTAGACCAGCACCATCTT

[1890] TGGTTACTCCAAAGACTCCAGCTGTATTCAAGACCATTGGTGTTCTGGAGTTCTACAGA

[1891] GGTTATTTGGCTAGAGAATTGAGAGGTAAATCTTACCTAGATACTATGAGAATCCAATCA

[1892] GAAGATGAGAAGAGTTCATAA

[1893] >SEQ ID NO:111

[1894] ATGGATGTCTCTTCTATCTTGGTTCCATCTGTCCAAGAATTGGCTAAAGAAGCTTTGACT

[1895] AGAGGTTCCAGAAAGATACGTCTTGAATCCAGATTCAAGATCCATTGCCATTGTCCAACAA

[1896] CTTCTGCTCCATTGCCTGATCAAGTTTCCAGTCATCGATTTGTCCAAGTTGTTGTCTCAAGA

[1897] TGATTTGAAAGGTCCAGATGAACTAGAGAAGTTGCATTCTGCTTGCAAAGATTGGGGT

[1898] TTCTTCCAACTAGTTAATCACGGTATCTCCACTTCTTTGGTTGAGAACATGAAAACCGG

[1899] TGTTAAGACTTTGTTCGAACTGTCTATGGATGAGAAGAAGCAGTTGTGGCAAAGAGAA

[1900] GGTGATTTGGAAGGCTTTGGTCAAGCTTTCATTGTCTCTGAAGAACAGAAGTTGGAAT

[1901] GGGCTGATGCTTTCTTCATGATCACTTTGCCACCACATTTGCGTATTCCACATTTGTTCA

[1902] ACCAAATTCCACAATCTTTCAGAGAGAATTTGGAAATCTACTCTGCTGAATTGGAGAAC

[1903] TTGGCTATCAAGATCATCGAATTGATGGCTAATGCTTTGGCTATTGATCCGAAAGAAATG

[1904] ACTGAAGTCTTTAAAGTCGGTATTCAGACTATCAGAGTCAACTACTATCCACCATGTCC

[1905] ACAACCAGAAAGAGTCATTGGTCTGAAATCTCATTCTGATTCTGGTGGTTTGACCATTC

[1906] TATTGCAAGTTAACGACATTGAAGGTTTGCAAATCAGAAAGGATGGTTTGTGGATTCCA

[1907] GTTCAACCATTGCCAAATGCCTTCATCATCAATATCGGTGATATGCTGGAAATTATGACT

[1908] AACGGTATCTACAGATCTATCGAACATAGAGCTACTGTCAATTCCGAGAAAGAACGTAT

[1909] TTCTTTGGCTACCTTCTATGCTCCATCTTTGGTCACTCCAGAAAGACCAGAACAATTTAG

[1910] AAGAATCTCTGTTGCTGATTACTTCAAGGGTTACTTCGATCCAAGAATTGTCTGGTAAGT

[1911] CTTACCTAGATGAAGTCAAGATTCCGAAAGGTGAATAA

[1912] >SEQ ID NO:112

[1913] ATGGATGCTCACTACATTGACTGTTCCATCTGTTCAAGAAATCGCTAAAGGCTCTTTGAC

[1914] AACAGTTCCAGAAAGATATGTTAGACCTCCACATGAAAGACCAACAACATTGTTCGCT

[1915] ACAACTTTGCCAGATCAAGTTCCAGTCATCGATTTGTCCAAGTTGCTGTCTCAAGATGA

[1916] CTTGGAAGGTCCAGCTGAATTAGAGAAATTACACTCTGCTTGTAAAGATTGGGGTTTCT

[1917] TCCAGTTGATCAATCATGGTGTCTCTATTTCACTATTAGAGAACGTTAAGGTGCTC

[1918] AAGAGTCTTTAACTTACCAATTGAAGAGAAGAAGAAGAATATTTGGTCAGAAAGAAG

[1919] GCGATGTTGAAGGTTATGGTCAAGCATTTGTCGTTTCTGAAGAACAGAAACTAGAATG

[1920] GGCAGACTTGTTCTACTTGTTAACCTTACCTCCACACATGAGAAAGCCACATTTGTTTC

[1921] CAAATCTGCCATTACCATTCAGAGATGATTTGGAGGCTTATACTGCTGAACTTAAGAACT

[1922] TGGCTATTCAAGTTCTTGATCTTATGGCTAATGCTTTGAAGATGGATGCCAAAGAAATGA

[1923] GAGAGTTGTTTGGTGAAGGTGTCCAATCCATGAGAATGAACTACTATCCACCATGTCCA

[1924] CAACCAGAATTGGTTATGGGTTTGAATCCACATTCTGATGGTGGTGGTTTGACTATATTG

[1925] TTACAAGCTAATGAAGTAGAAGGTTTGCAAATTAAGAAAGACGGTTTTGTGGATACCAG

[1926] TTAAACCACTTCCAAACGCATTTATCATTAACCTAGGTGATATGCTTGAAATGATCACTA

[1927] ATGGCATCTATAGAAGTATCGAACATAGAGCTACAGTTAACCTAGAGAAAGAAAGATTG

[1928] TCAATTGCTACCTTCTATAATCCAGGTGTTGAAATCACCGTTAGACCAGCACCATCTTTG

[1929] GTCACCAAAGACTCCAGCTGTATTCAAGACTATCGGTGTGTTAGAGTTCTATAGAGG

[1930] CTATTTCGCTAGAGAATTACAAGGTAAGAGTTACTTGGATACAATGAGAATACAATCAG

[1931] AAGATGAGAAATCTTCTTAA

[1932] >SEQ ID NO:113

[1933] ATGGATGCAACCTCTTTGACAGTACCTTCAAGTCCAAGAAATCGCTAAGGGTTCTTTGAC

[1934] TACTGTACCAGAAAGATACGTCAGACCTCCACATCAAAGACCAACAACTTTATTCGCTA

[1935] CTACTCCATTACCAGATCAAGTTCCAGTTATCGATCTGTCCAAATTGTTGTCTCAAGATG

[1936] ATCTTAAAGGTCCAGCTGAATTGGAGAAGTTACATTCCGCTTGTAAAGATTGGGGTTTC

[1937] TTTCAATTGATCAATCATGGTGTTTCAATCTCATTGCTGGAGAACGTCAAGAAAGGTGC

[1938] ACAAGAGTTCTTTAACTTGCCAATTGAAGAGAAGAAGAAGAAATTCGGCCAGAAAGA

[1939] AGGTGATGTTGAAGGTTATGGTCAAGCATTCGTTGTTTCAGAAGAACAGAAACTTGAA

[1940] TGGGCTGATTTGTTCTTCTTATTGACATTACCACCACACATGAGAAAGCCTCATTTGTTC

[1941] CCAAATTTGCCATTGCCATTTAGAGATGATTTGGAAGCTTACTGCTGAACTGAAGAA

[1942] CTTAGCCATACAAGTGTTGGATTTGATGGCTAATGCTTTAAAGGTTGATGCTAAAGAAAT

[1943] GAGAGAATTGTTTGGCGAAGGCGTTCAATGAGAATGAACTACTATCCACCTTGTC

[1944] CACAACCAGAATTGGTCATGGGTCTGAATCCACACATTGATGGTGGTGGTTTGACAATT

[1945] CTATTGCAAGCTAATGAAGTGGAAGGTTTGCAAATCAAGAAAGACGGTTTGTGGATTC

[1946] CAGTTAAACCTTTGCCAAACGCATTCATCATCAACTTGGGTGATATGTTGGAAATGATC

[1947] ACCAATGGTATTTACAGATCTATTGAACATAGAGCTACTGTTAATCTTGAGAAAGAAAG

[1948] ACTGTCCATCGCTACTTTCTATAATCCAGGTGTCGAAATCACTGTTAGACCTGCTCCATC

[1949] ATTGGTTACTCCAAGACTCCAGCTGTATTCAAGACCATCGGTGTCTTGGAGTTCTACA

[1950] GAGGTTACTTAGCTAGAGAATTGCAAGGTAAGAGCTATTTGGATACCATGAGAATCCAA

[1951] TCTGAAGATGAGAAGTCATCTTAA

[1952] >SEQ ID NO:114

[1953] ATGGACGTTTCTTCTATCTTGGTTCCATCTGTTCAAGAATTGGCTAAAGAAGCTTTGACT

[1954] AGAGGTTCCAGAAAGATACGTCTTGAATCCAGATTCAAGATCCATTGCCATTGTCTAACAA

[1955] CTCTGCACCATTGCCAGACCAAGTTCCAGTTATTGACTTGTCTAAGTTGTTGTCTCAAG

[1956] ATGATCTAAAGGGACCTGCTGAATTGGAGAAGTTGCATTCTGCTTGCAAAGACTGGGG

[1957] TTTCTTTCAACTAGTTAATCACGGTATCTCCACTTCTTTGGTTGAGAACATGAAAACTGG

[1958] TGTTAAGACTTTGTTTGAATTGTCTATGGATGAGAAGAAGCAACAATGGCAAAGAGAA

[1959] GGTGATTTGGAAGGTTTCGGTCAAGCTTTCATCGTTTCTGAAGAACAGAAGTTGGAAT

[1960] GGGCTGATGCTTTCTTCATGATCACTTTGCCACCACATTTGAGAAAGCCACATTTGTTC

[1961] AACCAGATTCCACAATCTTTCAGAGAGAACTTGGAAATCTACTCTGCTGAACTAGAGA

[1962] ATTCTAGCTATCAAGATCATCGAGTTGATGGCTAATGCTTTGGCTATTGATCCGAAAGAAA

[1963] TGACTGAAGTCTTTAAGGTTGGTACTCAAACCATTAGAGTTAACTACTATCCACCATGTC

[1964] CACAACCTGAAAGAGTCATCGGTCTGAAATCTCATTCCGATTCTGGTGGTTTGACCATC

[1965] TTGCTGCAAGTTAACGATATCGAAGGTTTGCAGATTAGAAAGGATGGTTTGTGGATTCC

[1966] AGTTCAACCATGCCTAACGCATTTATCATTAACATCGGTGATATGTTGGAAATCATGAC

[1967] CAATGGCATTTACAGATCTATTGAGCATAGAGCTACCGTTAACTCTGAGAAAGAAAGAA

[1968] TCTCTTTGGCTACTTTCTATGGTCCAAACATGCAAGCTATTCTAGCACCAGCACCATCTT

[1969] TGGTCACACCAGAAAGACCAGAACAATTCAGAAGAATCTCTGTTGCTGATTATTTCAA

[1970] GGGCTATTTCGACCAAGAATTGTCCGGTAAGTCTTACTTGGATGAAGTCAAGATTCCGA

[1971] AAGGTGAATAA

[1972] >SEQ ID NO:115

[1973] ATGACCAGCAATAGCGACTTAGTTAGGACTATAGAATCAGTATTAGGCGTCTCACTGGG

[1974] TGATTCCGTAAGTGACTCCCTAGTTCTAATCGCAACTACGTCCGTCGCTGTTATTATAGG

[1975] ACTTTTAGTTTTCTTATGGAAAAAATCTTCTGATCGTAGTAGAGAAGTTCGTCCGGTAAT

[1976] AGTTCCTAAATCTCTGGTCAAGGACGAAGACGATGATGTAGACGTTGCCTCCGGTAAA

[1977] ACTAAAGTTACGTTTTTTTTGGAACTCAGACCGGTACGGCGGAAGGTTTCGCAAAGG

[1978] CCTTGGCTGATGAAATCAAAGCACGTTATGAAAAAGCTTACGTTAAGGTAGTAGACCTA

[1979] GACGACTATGCGATGGACGATGATCAATACGAAGAAAAGCTTAAAAAGGAAACTCTTG

[1980] CTTTCTTCATGCTAGCCACTTATGGTGACGGTGAACCGACGGATAATGCTGCTAGATTCT

[1981] ATAAGTGGTTTACGGAAGGAAAAGAGGAACGTGGTACATGGTTGCAACAGTTGACCCA

[1982] CGGTGTTTTCGGTCTTGGCAACAAGCAATACGAACATTTTAACAAGATTGGTAAGGTTG

[1983] TAGACGAAGACCTTTCAGAACAAGGTGCGAAAAGACTTGTTCCTTTAGGATTGGGAGA

[1984] CGACGATCAATCAATCGAGGATGATTTCTCAGCCTGGAAAGAGAGTTTGTGGCCTGAG

[1985] TTAGACCAACTTCTAAGAGATGAAGATGATGTGAATACCGTCTCCACCCCATACACTGC

[1986] AGCTATTCCGGAATATAGGGTGGTCATTCATGACTCCACAGTTACACCTTCTTATGATAA

[1987] CCAATTTTCTGCTGCGAACGGTGGGGCAGTTTTCGACATTCATCATCCTTGCAGAGTAA

[1988] ACGTAGCAGTCAAAAGAGAACTTCATAAGCCCCAATCAGACAGAAGCTGTATACACCT

[1989] TGAATTCGATATTTCAGGGACAGGCATCACTTACGAGACTGGTGACCATGTTGGTGTTT

[1990] ATGCTGAAAATTGTGATGAAACGGTAGAGGAAGCCGGAAAATTGCTTGGTCAGAACTT

[1991] GGATTTGTTATTCTCTCTGCATACAGATAACGAGGATGGCACATCTCTAGGTGGTAGTTT

[1992] GTTACCACCCTTTCCAGGCCCATGTACCTTAAGAACAGCATTAGCCAGATACGCCGATC

[1993] TTCTAAATCCTCCAAGAAAGGCTGCCTTAGTGGTATTGGCTGCTCACGCGTCAGAACCT

[1994] TCCGAGGCTGAAAGATTGAAATTCTTATCCTCCCCCCAAGGTAAAGATGAATACAGCAA

[1995] GTGGGTCGTTGGTTCTCAAAGGTCCTTATTAGAAGTAATGGCAGAATTTCCTAGTGCTA

[1996] AACCACCTTTAGGTGTCTTTTTCGCTGCTATTGCGCCAAGATTGCAACCTAGATACTATT

[1997] CAATTTCCTCTTCACCGAGGTTTGCTAGTCAAAGGGTTCACGTTACATGTGCCTTGGTAT

[1998] ATGGTCCTACACCAACCGGCAGAATCCACAAGGGTGTCTGTTCAACTTGGATGAAAAA

[1999] CGCCATACCACTAGAAGAATCCAGAGATTGTGGCTGGGCCCCCATCTTCATTAGACCAT

[2000] CTAATTTTAAACTGCCTGCTGACCATTCTATTCCTATAATTATGGTTGGCCCTGGCACTGG

[2001] GTTGGCTCCTTTCAGGGGATTTTTACAAGAAAGATTTGCTTTGAAGGAAGACGGAGTG

[2002] CAATTGGGTCCATCTCTGTTGTTTTTCGGTTGCCGTAATAGGCAGATGGACTTCATTTAT

[2003] GAAGATGAATTGAAAAACTTCGTTGAGCAAGGTTCCTTAAGTGAGTTAATAGTTGCCTT

[2004] TTCTAGGGAAGGACCAGAAAAAGAGTACGTTCAACATAAAATGATGGATAAGGCTGCC

[2005] TACTTATGGTCATTGATTTCCCAAGGTGGATATTTACGTCTGCGGTGACGCTAAGGGT

[2006] ATGGCGAGAGATGTACATAGAATCTCTTCACACGATTGTGCAGCAGCAAGAGAACGTAG

[2007] AATCATCTAAAGCCGAAGCAATTGTAAAAAAGTTGCAAATGGACGGGAGATATTTAAG

[2008] AGATGTTTGGTAA

[2009] >SEQ ID NO:116

[2010] CAGTGAATTCGAAAAGGAGGTGCACGCATTTAT

[2011] >SEQ ID NO:117

[2012] AGGAGAAAAACTTCCAAGGAGGTGAAGAACGTC

[2013] >SEQ ID NO:118

[2014] GATATTGGAGGGATGGGACGTCAGCACTG

[2015] >SEQ ID NO:119

[2016] ctctagaggatccccgggtaCGGTATTACTCGAGCCCG

[2017] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the above embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

[2018] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "examples", "specific examples", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in a suitable manner in any one or more embodiments or examples. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of different embodiments or examples, unless they are mutually inconsistent. Although the embodiments of the present invention have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations of the present invention. Those skilled in the art may change, modify, replace and modify the above embodiments within the scope of the present invention.

Claims

1. A method for biosynthesis of glabridin, characterized in that: include: Provide a biological host; causing the biological host to express one or more enzymes selected from the group consisting of phenylalanine ammonia lyase, cinnamate-4-hydrogenase, tyrosine ammonia lyase, p-coumarate Coenzyme A ligase, chalcone synthase, chalcone reductase, chalcone isomerase, isoflavone synthase, 2-hydroxyisoflavone dehydratase, 4'-oxymethyltransferase, isoflavone 2'-hydrogenase, isoflavone reductase, visnezyl reductase, prenyltransferase, pterostilbene reductase, oxidative cyclase, demethylase, and cytochrome P450 reductase to obtain an engineered biological host; and The engineered biological host is cultured to obtain glabridin.

2. The biosynthesis method of glabridin according to claim 1, characterized in that: The phenylalanine ammonia lyase is derived from one or more of alfalfa and Glycyrrhiza glabra, wherein the phenylalanine ammonia lyase derived from alfalfa is MtPAL, whose accession number in the NCBI database is XP_003590471.1, and the phenylalanine ammonia lyase derived from Glycyrrhiza glabra is GgPAL, whose amino acid sequence is shown in SEQ ID NO: 1; Optionally, the cinnamate 4-hydrogenase is derived from one or more of Arabidopsis thaliana and Glycyrrhiza glabra, wherein the cinnamate 4-hydrogenase derived from Arabidopsis thaliana is AtC4H, whose accession number in the NCBI database is NP_180607.1, and the cinnamate 4-hydrogenase derived from Glycyrrhiza glabra is GgC4H, whose amino acid sequence is shown in SEQ ID NO: 2; Optionally, the tyrosine ammonia lyase is SeTAL derived from Saccharothrix hispanica, whose accession number in the NCBI database is ABC88669.1; Optionally, the p-coumarate-CoA ligase is derived from one or more of parsley and Glycyrrhiza glabra, wherein the p-coumarate-CoA ligase derived from parsley is Pc4CL, whose accession number in the NCBI database is CAA31697.1, and the p-coumarate-CoA ligase derived from Glycyrrhiza glabra is Gg4CL, whose amino acid sequence is shown in SEQ ID NO: 3; Optionally, the chalcone synthase is derived from one or more of petunia and Glycyrrhiza glabra, wherein the chalcone synthase derived from petunia is PhCHS, whose accession number in the NCBI database is CAA32731.1, and the chalcone synthase derived from Glycyrrhiza glabra is GgCHS, whose amino acid sequence is shown in SEQ ID NO: 4; Optionally, the chalcone reductase is GgCHR from Glycyrrhiza glabra, whose amino acid sequence is shown in SEQ ID NO: 5; Optionally, the chalcone isomerase is derived from one or more of alfalfa, Glycyrrhiza glabra, and Arabidopsis thaliana, wherein the chalcone isomerase derived from alfalfa is MsCHI, whose accession number in the NCBI database is AAB41524.1, the chalcone isomerase derived from Glycyrrhiza glabra is GgCHI, whose amino acid sequence is shown in SEQ ID NO: 6, and the chalcone isomerase derived from Arabidopsis thaliana is AtCHIL, whose accession number in the NCBI database is NP_850770.1; Optionally, the isoflavone synthase is GgIFS from Glycyrrhiza glabra, and its amino acid sequence is shown in SEQ ID NO: 7; Optionally, the 2-hydroxyisoflavone dehydratase is GgHID from Glycyrrhiza glabra, whose amino acid sequence is shown in SEQ ID NO: 8; Optionally, the 4'-O-methyltransferase is derived from one or more of Pueraria lobata and Glycyrrhiza glabra, wherein the 4'-O-methyltransferase derived from Pueraria lobata is P1OMT9, whose accession number in the NCBI database is AKW47171.1, and the 4'-O-methyltransferase derived from Glycyrrhiza glabra is GgOMT, whose amino acid sequence is shown in SEQ ID NO: 9; Optionally, the isoflavone 2'-hydrogenase is derived from one or more of soybean and Glycyrrhiza glabra, wherein the soybean-derived isoflavone 2'-hydrogenase is GeCYP81E1, whose UniProtKB / Swiss-Prot number is P93147.2, and the Glycyrrhiza glabra-derived isoflavone 2'-hydrogenase is GgI2'H, whose amino acid sequence is shown in SEQ ID NO: 10; Optionally, the isoflavone reductase is derived from one or more of alfalfa and Glycyrrhiza glabra, wherein the isoflavone reductase derived from alfalfa is MsIFR, whose GenBank number is CAA41106.1, and the isoflavone reductase derived from Glycyrrhiza glabra is GgIFR, whose amino acid sequence is shown in SEQ ID NO: 11; Optionally, the visconazole reductase is derived from one or more of alfalfa and Glycyrrhiza glabra, wherein the visconazole reductase derived from alfalfa is MsVR, whose UniProtKB / Swiss-Prot number is Q40316.1, and the visconazole reductase derived from Glycyrrhiza glabra is GgVR, whose amino acid sequence is shown in SEQ ID NO: 12; Optionally, the prenyltransferase is derived from one or more of Psoralea corylifolia and Glycyrrhiza glabra, wherein the prenyltransferase derived from Psoralea corylifolia is tPcM4DT, whose amino acid sequence is shown in SEQ ID NO: 13, and the prenyltransferase derived from Glycyrrhiza glabra is one or more of GgPT1, GgPT2, GgPT3, GgPT4, GgPT5, GgPT6, and GgPT7, whose amino acid sequences are shown in SEQ ID NOs: 14, 15, 16, 17, 18, 19, and 20, respectively; Optionally, the pterostilbene reductase is one or more of GgPTR1, GgPTR2, GgPTR3, GgPTR4, GgPTR5, GgPTR6, GgPTR7, and GgPTR8 derived from Glycyrrhiza glabra, and the amino acid sequences thereof are shown in SEQ ID NOs: 21, 22, 23, 24, 25, 26, 27, and 28, respectively; Optionally, the oxidative cyclase is one or more of GgOC1, GgOC2, GgOC3, GgOC4, GgOC5, GgOC6, GgOC7, and GgOC8 from Glycyrrhiza glabra, and the amino acid sequences thereof are shown in SEQ ID NOs: 29, 30, 31, 32, 33, 34, 35, and 36, respectively; Optionally, the demethylase is derived from one or more of Fusarium solani, Glycyrrhiza glabra, Glycyrrhiza uralensis, and Glycyrrhiza inflata, wherein the demethylase derived from Fusarium solani is NhDMT, and its amino acid sequence is shown in SEQ ID NO: 37; the demethylase derived from Glycyrrhiza glabra is one or more of GgDMT1, GgDMT2, GgDMT3, GgDMT4, GgDMT5, GgDMT6, GgDMT7, and GgDMT8, and their amino acid sequences are shown in SEQ ID NOs: 38, 39, 40, 41, 42, 43, 44, and 45, respectively; the demethylase derived from Glycyrrhiza uralensis is one or more of GuDMT1, GuDMT2, and GuDMT3, and their amino acid sequences are shown in SEQ ID NOs: As shown in SEQ ID NOs: 46, 47, and 48, the demethylase derived from Glycyrrhiza inflata is one or more of GiDMT1, GiDMT2, and GiDMT3, and the amino acid sequences thereof are shown in SEQ ID NOs: 49, 50, and 51, respectively; Optionally, the cytochrome P450 reductase is GuCPR2 derived from Glycyrrhiza uralensis, and its amino acid sequence is shown in SEQ ID NO:

52.

3. The biosynthesis method of glabridin according to claim 2, characterized in that: At least one of the phenylalanine ammonia lyase, cinnamate-4-hydrogenase, tyrosine ammonia lyase, p-coumarate Coenzyme A ligase, chalcone synthase, chalcone reductase, chalcone isomerase, isoflavone synthase, 2-hydroxyisoflavone dehydratase, 4'-oxymethyltransferase, isoflavone 2'-hydrogenase, isoflavone reductase, visconol reductase, prenyltransferase, pterostilbene reductase, oxidative cyclase, demethylase and cytochrome P450 reductase is derived from Glycyrrhiza glabra.

4. The biosynthesis method of glabridin according to claim 1, characterized in that: The biological host is selected from the group consisting of: Gram-positive bacteria, Gram-negative bacteria, actinomycetes, yeast, filamentous fungi and tobacco; Optionally, the gram-positive bacteria include Bacillus subtilis and Corynebacterium glutamicum, the gram-negative bacteria include Escherichia coli, the actinomycetes include Streptomyces coelicolor, the yeast include Saccharomyces cerevisiae, Yarrowia lipolytica and Pichia pastoris, the filamentous fungi include Aspergillus niger, and the tobacco includes Nicotiana benthamiana.

5. The biosynthesis method of glabridin according to claim 1, characterized in that: The biological host is yeast, The engineered biological host heterologously expresses: Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase tPcM4DT from Psoralea corylifolia, pterostilbene reductase GgPTR1 from Glycyrrhiza glabra, oxidocyclase GgOC1 from Glycyrrhiza glabra, demethylase NhDMT from Fusarium solani; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT1 from Glycyrrhiza glabra, pterostilbene reductase GgPTR2 from Glycyrrhiza glabra, oxidative cyclase GgOC2 from Glycyrrhiza glabra, demethylase GgDMT1 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT2 from Glycyrrhiza glabra, pterostilbene reductase GgPTR3 from Glycyrrhiza glabra, oxidative cyclase GgOC3 from Glycyrrhiza glabra, demethylase GgDMT2 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT3 from Glycyrrhiza glabra, pterostilbene reductase GgPTR4 from Glycyrrhiza glabra, oxidative cyclase GgOC4 from Glycyrrhiza glabra, demethylase GgDMT3 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT4 from Glycyrrhiza glabra, pterostilbene reductase GgPTR5 from Glycyrrhiza glabra, oxidocyclase GgOC5 from Glycyrrhiza glabra, demethylase GgDMT4 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT5 from Glycyrrhiza glabra, pterostilbene reductase GgPTR6 from Glycyrrhiza glabra, oxidative cyclase GgOC6 from Glycyrrhiza glabra, demethylase GgDMT5 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT6 from Glycyrrhiza glabra, pterostilbene reductase GgPTR7 from Glycyrrhiza glabra, oxidocyclase GgOC7 from Glycyrrhiza glabra, demethylase GgDMT6 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT7 from Glycyrrhiza glabra, pterostilbene reductase GgPTR8 from Glycyrrhiza glabra, oxidocyclase GgOC8 from Glycyrrhiza glabra, demethylase GgDMT7 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT1 from Glycyrrhiza glabra, pterostilbene reductase GgPTR2 from Glycyrrhiza glabra, oxidative cyclase GgOC2 from Glycyrrhiza glabra, demethylase GgDMT8 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT2 from Glycyrrhiza glabra, pterostilbene reductase GgPTR3 from Glycyrrhiza glabra, oxidative cyclase GgOC3 from Glycyrrhiza glabra, demethylase GuDMT1 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT3 from Glycyrrhiza glabra, pterostilbene reductase GgPTR4 from Glycyrrhiza glabra, oxidocyclase GgOC4 from Glycyrrhiza glabra, demethylase GuDMT2 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT4 from Glycyrrhiza glabra, pterostilbene reductase GgPTR5 from Glycyrrhiza glabra, oxidocyclase GgOC5 from Glycyrrhiza glabra, demethylase GuDMT3 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT5 from Glycyrrhiza glabra, pterostilbene reductase GgPTR6 from Glycyrrhiza glabra, oxidative cyclase GgOC6 from Glycyrrhiza glabra, demethylase GiDMT1 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT6 from Glycyrrhiza glabra, pterostilbene reductase GgPTR7 from Glycyrrhiza glabra, oxidocyclase GgOC7 from Glycyrrhiza glabra, demethylase GiDMT2 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIF from Glycyrrhiza glabra S, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT7 from Glycyrrhiza glabra, pterostilbene reductase GgPTR8 from Glycyrrhiza glabra, oxidative cyclase GgOC8 from Glycyrrhiza glabra, demethylase GiDMT3 from Glycyrrhiza glabra; Wherein, the substrate for biosynthesis of glabridin is selected from the group consisting of glucose, galactose and xylose.

6. The biosynthesis method of glabridin according to claim 5, characterized in that: The engineered biological host heterologously expresses: Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase tPcM4DT from Psoralea corylifolia, pterostilbene reductase GgPTR1 from Glycyrrhiza glabra, oxidocyclase GgOC1 from Glycyrrhiza glabra, demethylase NhDMT from Fusarium solani; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT1 from Glycyrrhiza glabra, pterostilbene reductase GgPTR2 from Glycyrrhiza glabra, oxidative cyclase GgOC2 from Glycyrrhiza glabra, demethylase GgDMT1 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT2 from Glycyrrhiza glabra, pterostilbene reductase GgPTR3 from Glycyrrhiza glabra, oxidative cyclase GgOC3 from Glycyrrhiza glabra, demethylase GgDMT2 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT3 from Glycyrrhiza glabra, pterostilbene reductase GgPTR4 from Glycyrrhiza glabra, oxidative cyclase GgOC4 from Glycyrrhiza glabra, demethylase GgDMT3 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT1 from Glycyrrhiza glabra, pterostilbene reductase GgPTR2 from Glycyrrhiza glabra, oxidative cyclase GgOC2 from Glycyrrhiza glabra, demethylase GgDMT8 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT2 from Glycyrrhiza glabra, pterostilbene reductase GgPTR3 from Glycyrrhiza glabra, oxidative cyclase GgOC3 from Glycyrrhiza glabra, demethylase GuDMT1 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIF from Glycyrrhiza glabra S, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, isoprenyltransferase GgPT3 from Glycyrrhiza glabra, pterostilbene reductase GgPTR4 from Glycyrrhiza glabra, oxidative cyclase GgOC4 from Glycyrrhiza glabra, demethylase GuDMT2 from Glycyrrhiza glabra.

7. The biosynthesis method of glabridin according to any one of claims 1 to 6, characterized in that: Also includes: The biological host is caused to express: a mutated 4'-O-methyltransferase derived from kudzu vine comprising one or more of the following mutations: F24Y, F307L, F307I, F307V, M311L, Y160P, and Y160L, wherein the amino acid sequence of the 4'-O-methyltransferase derived from kudzu vine has an accession number of AKW47171.1 in the NCBI database; Optionally, the method for biosynthesizing glabridin further comprises: causing the biological host to express: a mutated ARO4 from Saccharomyces cerevisiae comprising the following mutation: K229L; and / or a mutated ARO7 from Saccharomyces cerevisiae comprising the following mutation: G141S, wherein the accession number of ARO4 from Saccharomyces cerevisiae in the NCBI database is NP_009808.1, and the accession number of ARO7 from Saccharomyces cerevisiae in the NCBI database is NP_015385.1; Optionally, the method for biosynthesis of glabridin further comprises: causing the biological host to heterologously express or overexpress: a synthase of dimethylallyl pyrophosphate, wherein the synthesis gene of dimethylallyl pyrophosphate is selected from the group consisting of: acetyl-CoA acetyltransferase, 3-hydroxy-3-methylglutaryl-CoA synthase, hydroxymethylglutaryl-CoA reductase, mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylase, and dimethylallyl diphosphate isomerase; Optionally, the biosynthesis method of glabridin further comprises: causing the biological host to heterologously express or overexpress an enzyme selected from the group consisting of: 6-phosphate glucose dehydrogenase, malate dehydrogenase, and NADH-dependent hydroxymethylglutaryl-CoA reductase, wherein the accession number of the NCBI database for the 6-phosphate glucose dehydrogenase is NP_014158.1, the accession number of the NCBI database for the malate dehydrogenase is NP_012896.1, and the accession number of the NADH-dependent hydroxymethylglutaryl-CoA reductase is WP_011241944.1; Optionally, the biosynthesis method of glabridin further comprises: causing the biological host to heterologously express or overexpress acetyl-CoA carboxylase; Optionally, the biosynthesis method of glabridin further comprises: causing the biological host to express a demethylase using a galactose-inducible promoter or a xylose-inducible promoter.

8. An engineered biological host, characterized in that The engineered host organism expresses one or more enzymes selected from the group consisting of: phenylalanine ammonia lyase, cinnamate-4-hydrogenase, tyrosine ammonia lyase, p-coumarate CoA ligase, chalcone synthase, chalcone reductase, chalcone isomerase, isoflavone synthase, 2-hydroxyisoflavone dehydratase, 4'-oxymethyltransferase, isoflavone 2'-hydrogenase, isoflavone reductase, visnezyl reductase, prenyltransferase, pterostilbene reductase, oxidative cyclase, demethylase, cytochrome P450 reductase, At least one of the phenylalanine ammonia lyase, cinnamate 4-hydrogenase, tyrosine ammonia lyase, p-coumarate Coenzyme A ligase, chalcone synthase, chalcone reductase, chalcone isomerase, isoflavone synthase, 2-hydroxyisoflavone dehydratase, 4'-oxymethyltransferase, isoflavone 2'-hydrogenase, isoflavone reductase, visconol reductase, prenyltransferase, pterostilbene reductase, oxidative cyclase, demethylase and cytochrome P450 reductase is derived from Glycyrrhiza glabra.

9. The modified biological host according to claim 8, characterized in that The phenylalanine ammonia lyase is derived from one or more of alfalfa and Glycyrrhiza glabra, wherein the phenylalanine ammonia lyase derived from alfalfa is MtPAL, whose accession number in the NCBI database is XP_003590471.1, and the phenylalanine ammonia lyase derived from Glycyrrhiza glabra is GgPAL, whose amino acid sequence is shown in SEQ ID NO: 1; Optionally, the cinnamate 4-hydrogenase is derived from one or more of Arabidopsis thaliana and Glycyrrhiza glabra, wherein the cinnamate 4-hydrogenase derived from Arabidopsis thaliana is AtC4H, whose accession number in the NCBI database is NP_180607.1, and the cinnamate 4-hydrogenase derived from Glycyrrhiza glabra is GgC4H, whose amino acid sequence is shown in SEQ ID NO: 2; Optionally, the tyrosine ammonia lyase is SeTAL derived from Saccharothrix hispanica, whose accession number in the NCBI database is ABC88669.1; Optionally, the p-coumarate-CoA ligase is derived from one or more of parsley and Glycyrrhiza glabra, wherein the p-coumarate-CoA ligase derived from parsley is Pc4CL, whose accession number in the NCBI database is CAA31697.1, and the p-coumarate-CoA ligase derived from Glycyrrhiza glabra is Gg4CL, whose amino acid sequence is shown in SEQ ID NO: 3; Optionally, the chalcone synthase is derived from one or more of petunia and Glycyrrhiza glabra, wherein the chalcone synthase derived from petunia is PhCHS, whose accession number in the NCBI database is CAA32731.1, and the chalcone synthase derived from Glycyrrhiza glabra is GgCHS, whose amino acid sequence is shown in SEQ ID NO: 4; Optionally, the chalcone reductase is GgCHR from Glycyrrhiza glabra, whose amino acid sequence is shown in SEQ ID NO: 5; Optionally, the chalcone isomerase is derived from one or more of alfalfa, Glycyrrhiza glabra, and Arabidopsis thaliana, wherein the chalcone isomerase derived from alfalfa is MsCHI, whose accession number in the NCBI database is AAB41524.1, the chalcone isomerase derived from Glycyrrhiza glabra is GgCHI, whose amino acid sequence is shown in SEQ ID NO: 6, and the chalcone isomerase derived from Arabidopsis thaliana is AtCHIL, whose accession number in the NCBI database is NP_850770.1; Optionally, the isoflavone synthase is GgIFS from Glycyrrhiza glabra, and its amino acid sequence is shown in SEQ ID NO: 7; Optionally, the 2-hydroxyisoflavone dehydratase is GgHID from Glycyrrhiza glabra, whose amino acid sequence is shown in SEQ ID NO: 8; Optionally, the 4'-O-methyltransferase is derived from one or more of Pueraria lobata and Glycyrrhiza glabra, wherein the 4'-O-methyltransferase derived from Pueraria lobata is P1OMT9, whose accession number in the NCBI database is AKW47171.1, and the 4'-O-methyltransferase derived from Glycyrrhiza glabra is GgOMT, whose amino acid sequence is shown in SEQ ID NO: 9; Optionally, the isoflavone 2'-hydrogenase is derived from one or more of soybean and Glycyrrhiza glabra, wherein the soybean-derived isoflavone 2'-hydrogenase is GeCYP81E1, whose UniProtKB / Swiss-Prot number is P93147.2, and the Glycyrrhiza glabra-derived isoflavone 2'-hydrogenase is GgI2'H, whose amino acid sequence is shown in SEQ ID NO: 10; Optionally, the isoflavone reductase is derived from one or more of alfalfa and Glycyrrhiza glabra, wherein the isoflavone reductase derived from alfalfa is MsIFR, whose accession number in the NCBI database is CAA41106.1, and the isoflavone reductase derived from Glycyrrhiza glabra is GgIFR, whose amino acid sequence is shown in SEQ ID NO: 11; Optionally, the visconazole reductase is derived from one or more of alfalfa and Glycyrrhiza glabra, wherein the visconazole reductase derived from alfalfa is MsVR, whose UniProtKB / Swiss-Prot number is Q40316.1, and the visconazole reductase derived from Glycyrrhiza glabra is GgVR, whose amino acid sequence is shown in SEQ ID NO: 12; Optionally, the prenyltransferase is derived from one or more of Psoralea corylifolia and Glycyrrhiza glabra, wherein the prenyltransferase derived from Psoralea corylifolia is tPcM4DT, whose amino acid sequence is shown in SEQ ID NO: 13, and the prenyltransferase derived from Glycyrrhiza glabra is one or more of GgPT1, GgPT2, GgPT3, GgPT4, GgPT5, GgPT6, and GgPT7, whose amino acid sequences are shown in SEQ ID NOs: 14, 15, 16, 17, 18, 19, and 20, respectively; Optionally, the pterostilbene reductase is one or more of GgPTR1, GgPTR2, GgPTR3, GgPTR4, GgPTR5, GgPTR6, GgPTR7, and GgPTR8 derived from Glycyrrhiza glabra, and the amino acid sequences thereof are shown in SEQ ID NOs: 21, 22, 23, 24, 25, 26, 27, and 28, respectively; Optionally, the oxidative cyclase is one or more of GgOC1, GgOC2, GgOC3, GgOC4, GgOC5, GgOC6, GgOC7, and GgOC8 from Glycyrrhiza glabra, and the amino acid sequences thereof are shown in SEQ ID NOs: 29, 30, 31, 32, 33, 34, 35, and 36, respectively; Optionally, the demethylase is derived from one or more of Fusarium solani, Glycyrrhiza glabra, Glycyrrhiza uralensis, and Glycyrrhiza inflata, wherein the demethylase derived from Fusarium solani is NhDMT, and its amino acid sequence is shown in SEQ ID NO: 37; the demethylase derived from Glycyrrhiza glabra is one or more of GgDMT1, GgDMT2, GgDMT3, GgDMT4, GgDMT5, GgDMT6, GgDMT7, and GgDMT8, and their amino acid sequences are shown in SEQ ID NOs: 38, 39, 40, 41, 42, 43, 44, and 45, respectively; the demethylase derived from Glycyrrhiza uralensis is one or more of GuDMT1, GuDMT2, and GuDMT3, and their amino acid sequences are shown in SEQ ID NOs: As shown in SEQ ID NOs: 46, 47, and 48, the demethylase derived from Glycyrrhiza inflata is one or more of GiDMT1, GiDMT2, and GiDMT3, and the amino acid sequences thereof are shown in SEQ ID NOs: 49, 50, and 51, respectively; Optionally, the cytochrome P450 reductase is GuCPR2 derived from Glycyrrhiza uralensis, and its amino acid sequence is shown in SEQ ID NO:

52.

10. The engineered biological host according to claim 8 or 9, characterized in that The engineered biological host heterologously expresses: Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase tPcM4DT from Psoralea corylifolia, pterostilbene reductase GgPTR1 from Glycyrrhiza glabra, oxidocyclase GgOC from Glycyrrhiza glabra, demethylase NhDMT from Fusarium solani; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIF from Glycyrrhiza glabra S, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT from Glycyrrhiza glabra, pterostilbene reductase GgPTR2 from Glycyrrhiza glabra, oxidative cyclase GgOC from Glycyrrhiza glabra, demethylase GgDMT1 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT2 from Glycyrrhiza glabra, pterostilbene reductase GgPTR3 from Glycyrrhiza glabra, oxidative cyclase GgOC3 from Glycyrrhiza glabra, demethylase GgDMT2 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT3 from Glycyrrhiza glabra, pterostilbene reductase GgPTR4 from Glycyrrhiza glabra, oxidative cyclase GgOC4 from Glycyrrhiza glabra, demethylase GgDMT3 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT4 from Glycyrrhiza glabra, pterostilbene reductase GgPTR5 from Glycyrrhiza glabra, oxidocyclase GgOC5 from Glycyrrhiza glabra, demethylase GgDMT4 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT5 from Glycyrrhiza glabra, pterostilbene reductase GgPTR6 from Glycyrrhiza glabra, oxidative cyclase GgOC6 from Glycyrrhiza glabra, demethylase GgDMT5 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT6 from Glycyrrhiza glabra, pterostilbene reductase GgPTR7 from Glycyrrhiza glabra, oxidocyclase GgOC7 from Glycyrrhiza glabra, demethylase GgDMT6 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT7 from Glycyrrhiza glabra, pterostilbene reductase GgPTR8 from Glycyrrhiza glabra, oxidocyclase GgOC8 from Glycyrrhiza glabra, demethylase GgDMT7 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT1 from Glycyrrhiza glabra, pterostilbene reductase GgPTR2 from Glycyrrhiza glabra, oxidative cyclase GgOC2 from Glycyrrhiza glabra, demethylase GgDMT8 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT2 from Glycyrrhiza glabra, pterostilbene reductase GgPTR3 from Glycyrrhiza glabra, oxidative cyclase GgOC3 from Glycyrrhiza glabra, demethylase GuDMT1 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT3 from Glycyrrhiza glabra, pterostilbene reductase GgPTR4 from Glycyrrhiza glabra, oxidocyclase GgOC4 from Glycyrrhiza glabra, demethylase GuDMT2 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT4 from Glycyrrhiza glabra, pterostilbene reductase GgPTR5 from Glycyrrhiza glabra, oxidocyclase GgOC5 from Glycyrrhiza glabra, demethylase GuDMT3 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT5 from Glycyrrhiza glabra, pterostilbene reductase GgPTR6 from Glycyrrhiza glabra, oxidative cyclase GgOC6 from Glycyrrhiza glabra, demethylase GiDMT1 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra , 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT6 from Glycyrrhiza glabra, pterostilbene reductase GgPTR7 from Glycyrrhiza glabra, oxidocyclase GgOC7 from Glycyrrhiza glabra, demethylase GiDMT2 from Glycyrrhiza glabra; or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIF from Glycyrrhiza glabra S, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT7 from Glycyrrhiza glabra, pterostilbene reductase GgPTR8 from Glycyrrhiza glabra, oxidative cyclase GgOC8 from Glycyrrhiza glabra, demethylase GiDMT3 from Glycyrrhiza glabra; Optionally, the modified biological host expresses a mutated 4'-O-methyltransferase from kudzu comprising one or more of the following mutations: F24Y, F307L, F307I, F307V, M311L, Y160P, and Y160L, wherein the amino acid sequence of the 4'-O-methyltransferase from kudzu has an accession number of AKW47171.1 in the NCBI database; Optionally, the modified biological host expresses: a mutated ARO4 from Saccharomyces cerevisiae comprising the following mutation: K229L; and / or a mutated ARO7 from Saccharomyces cerevisiae comprising the following mutation: G141S, wherein the accession number of ARO4 from Saccharomyces cerevisiae in the NCBI database is NP_009808.1, and the accession number of ARO7 from Saccharomyces cerevisiae in the NCBI database is NP_015385.1; Optionally, the modified biological host heterologously expresses or overexpresses: a synthase of dimethylallyl pyrophosphate, wherein the synthesis gene of dimethylallyl pyrophosphate is selected from the group consisting of: acetyl-CoA acetyltransferase, 3-hydroxy-3-methylglutaryl-CoA synthase, hydroxymethylglutaryl-CoA reductase, mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylase and dimethylallyl diphosphate isomerase; Optionally, the modified biological host heterologously expresses or overexpresses an enzyme selected from the group consisting of: 6-phosphate glucose dehydrogenase, malate dehydrogenase and NADH-dependent hydroxymethylglutaryl-CoA reductase, wherein the accession number of the 6-phosphate glucose dehydrogenase in the NCBI database is NP_014158.1, the accession number of the malate dehydrogenase in the NCBI database is NP_012896.1, and the accession number of the NADH-dependent hydroxymethylglutaryl-CoA reductase in the NCBI database is WP_011241944.1; Optionally, the modified biological host heterologously expresses or overexpresses acetyl-CoA carboxylase; Optionally, the engineered biological host expresses the demethylase with a galactose-inducible promoter or a xylose-inducible promoter.

11. The engineered biological host according to claim 10, characterized in that The engineered biological host heterologously expresses: Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase tPcM4DT from Psoralea corylifolia, pterostilbene reductase GgPTR1 from Glycyrrhiza glabra, oxidative cyclase GgOC1 from Glycyrrhiza glabra, demethylase NhDMT from Fusarium solani (corresponding to GLA1 in the examples of the present application); or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT1 from Glycyrrhiza glabra, pterostilbene reductase GgPTR2 from Glycyrrhiza glabra, oxidative cyclase GgOC2 from Glycyrrhiza glabra, demethylase GgDMT1 from Glycyrrhiza glabra (corresponding to GLA2 in the examples of the present application); or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT2 from Glycyrrhiza glabra, pterostilbene reductase GgPTR3 from Glycyrrhiza glabra, oxidative cyclase GgOC3 from Glycyrrhiza glabra, demethylase GgDMT2 from Glycyrrhiza glabra (corresponding to GLA3 in the examples of the present application); or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, isoprenyltransferase GgPT3 from Glycyrrhiza glabra, pterostilbene reductase GgPTR4 from Glycyrrhiza glabra, oxidative cyclase GgOC4 from Glycyrrhiza glabra, demethylase GgDMT3 from Glycyrrhiza glabra (corresponding to GLA4 in the examples of the present application); or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT1 from Glycyrrhiza glabra, pterostilbene reductase GgPTR2 from Glycyrrhiza glabra, oxidative cyclase GgOC2 from Glycyrrhiza glabra, demethylase GgDMT8 from Glycyrrhiza glabra (corresponding to GLA9 in the examples of the present application); or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SeTAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, prenyltransferase GgPT2 from Glycyrrhiza glabra, pterostilbene reductase GgPTR3 from Glycyrrhiza glabra, oxidative cyclase GgOC3 from Glycyrrhiza glabra, demethylase GuDMT1 from Glycyrrhiza glabra (corresponding to GLA10 in the examples of the present application); or Phenylalanine ammonia lyase GgPAL from Glycyrrhiza glabra, cinnamate-4-hydrogenase GgC4H from Glycyrrhiza glabra, tyrosine ammonia lyase SetAL from Saccharothrix hispanica, p-coumarate-CoA ligase Gg4CL from Glycyrrhiza glabra, chalcone synthase GgCHS from Glycyrrhiza glabra, chalcone reductase GgCHR from Glycyrrhiza glabra, chalcone isomerase GgCHI from Glycyrrhiza glabra, cytochrome P450 reductase GuCPR2 from Glycyrrhiza uralensis, isoflavone synthase GgIFS from Glycyrrhiza glabra, 2-hydroxyisoflavone dehydratase GgHID from Glycyrrhiza glabra, 4'-oxymethyltransferase GgOMT from Glycyrrhiza glabra, isoflavone 2'-hydrogenase GgI2'H from Glycyrrhiza glabra, isoflavone reductase GgIFR from Glycyrrhiza glabra, visconol reductase GgVR from Glycyrrhiza glabra, isoprenyltransferase GgPT3 from Glycyrrhiza glabra, pterostilbene reductase GgPTR4 from Glycyrrhiza glabra, oxidative cyclase GgOC4 from Glycyrrhiza glabra, demethylase GuDMT2 from Glycyrrhiza glabra (corresponding to GLA11 in the examples of the present application).

12. The engineered biological host according to claim 8, characterized in that The biological host is selected from the group consisting of: Gram-positive bacteria, Gram-negative bacteria, actinomycetes, yeast, filamentous fungi and tobacco; Optionally, the gram-positive bacteria include Bacillus subtilis and Corynebacterium glutamicum, the gram-negative bacteria include Escherichia coli, the actinomycetes include Streptomyces coelicolor, the yeast include Saccharomyces cerevisiae, Yarrowia lipolytica and Pichia pastoris, the filamentous fungi include Aspergillus niger, and the tobacco includes Nicotiana benthamiana.

13. The engineered biological host according to claim 8, characterized in that The engineered biological host is Saccharomyces cerevisiae Glabd-831 with a deposit number of CGMCC No. 33965.

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