A plant-derived composite antibacterial agent and its application

Abstract

The invention discloses a plant-based composite antibacterial agent and an application thereof. The plant-based composite antibacterial agent comprises the following raw materials in percentage by mass: 20-60% of an antibacterial agent, wherein the antibacterial agent comprises a mixture of at least two of a forsythia fruit extract, an artemisia capillaris extract and a raspberry extract. The invention can significantly improve the antibacterial and anti-inflammatory effects by giving full play to the synergistic effect between the extracts of the antibacterial agent, and can achieve the purpose of rapid and long-term antibacterial effect. The plant-based multi-effect composition can be used in cosmetics and has the advantages of low dosage, safety, effectiveness, natural low stimulation and the like.

Description

Technical Field

[0001] The invention belongs to the field of antibacterial agents, and in particular relates to a plant-derived composite antibacterial agent and application thereof. Background Art

[0002] In recent years, with the improvement of living standards, people pay more and more attention to the maintenance of their skin, and the use of cosmetics and skin care products has become more frequent. Preservatives are an indispensable ingredient in cosmetics. Adding preservatives can inhibit the growth of microorganisms in cosmetics. Selecting efficient and safe preservatives is one of the keys to the development of cosmetic formulas. However, existing preservatives are difficult to maintain good anti-inflammatory effects while maintaining good preservative effects. Therefore, the importance of developing a natural extract preservative with good anti-inflammatory effects is becoming increasingly prominent.

[0003] Forsythia fruit is one of the commonly used Chinese medicines and has been included in pharmacopoeias of all dynasties. It has antibacterial, cardiotonic, diuretic and antiemetic pharmacological effects. Forsythia is often used to treat acute wind-heat colds, carbuncles, lymph node tuberculosis, urinary tract infections and other symptoms. It is one of the main raw materials for Chinese medicine preparations.

[0004] Artemisia capillaris, also known as Yinchen, Yinchen, and Artemisia capillaris, is a herbaceous plant of the genus Artemisia in the Asteraceae family. Its volatile oil is mainly used to treat rheumatism, cold and heat, evil qi heat accumulation, jaundice, etc. Its water extract has an inhibitory effect on a variety of bacilli and cocci, and its volatile oil has an antifungal effect; it can be used as a substitute for Artemisia annua and used as medicine; its young branches and leaves can be used as vegetables or brewed into Artemisia capillaris wine; and its hay can be used as livestock feed.

[0005] Raspberry is the dried immature fruit of the Rosaceae plant Rubus idaeus. It has been confirmed that raspberry has many medicinal values ​​such as antioxidant, anti-tumor, antibacterial and anti-inflammatory, analgesic and antitussive, lipid-lowering and blood sugar-lowering. Its main functional ingredients include phenolic substances, flavonoids, triterpenes, minerals, vitamins, carotenoids and organic acids. Summary of the invention

[0006] In view of the deficiencies in the above-mentioned prior art, the present invention can significantly improve the antibacterial and anti-inflammatory effects and achieve the purpose of rapid and long-term antibacterial by giving full play to the synergistic effect between the extracts of the antibacterial agent. The plant extract multi-effect composition can be used in cosmetics and has the advantages of low dosage, safety and effectiveness, and natural low irritation.

[0007] The object of the present invention is to provide a plant-derived composite antibacterial agent, comprising the following raw materials in percentage by weight: 20-60% of an antibacterial agent, wherein the antibacterial agent comprises a mixture of at least two of a Forsythia suspensa fruit extract, an Artemisia capillaris extract and a raspberry extract.

[0008] In some embodiments of the present invention, the plant-derived composite antibacterial agent comprises the following raw materials in percentage by weight: 30-50% of the antibacterial agent, wherein the antibacterial agent comprises a mixture of at least two of Forsythia suspensa fruit extract, Artemisia capillaris extract, and Raspberry extract.

[0009] In some embodiments of the present invention, the plant-derived composite antibacterial agent comprises the following raw materials in percentage by weight: 40% of the antibacterial agent, wherein the antibacterial agent comprises a mixture of at least two of Forsythia suspensa fruit extract, Artemisia capillaris extract, and Raspberry extract.

[0010] In some embodiments of the present invention, the plant-derived composite antibacterial agent further comprises 40-80% of a solvent.

[0011] In some embodiments of the present invention, the plant-derived composite antibacterial agent further comprises 50-70% of a solvent.

[0012] In some embodiments of the present invention, the plant-derived composite antibacterial agent further comprises 60% of a solvent.

[0013] In some embodiments of the present invention, the solvent includes at least one of 1,3-butanediol, 1,3-propylene glycol, 1,2-propylene glycol, 1,2-butanediol, 1,4-butanediol, 1,2-pentanediol, 1,5-pentanediol, isopentyl glycol, isohexanediol, 1,2-hexanediol, dipropylene glycol, water, glycerol, and methylpropanediol.

[0014] In some embodiments of the present invention, the mass ratio of the Forsythia suspensa fruit extract to the Artemisia capillaris extract is 5-15:4-6.

[0015] In some embodiments of the present invention, the mass ratio of the Forsythia suspensa fruit extract to the Artemisia capillaris extract is 3:1.

[0016] In some embodiments of the present invention, the mass ratio of the Forsythia suspensa fruit extract to the Rubus idaeus extract is 1-3:1-3.

[0017] In some embodiments of the present invention, the mass ratio of the Forsythia suspensa fruit extract to the Rubus idaeus extract is 3:5.

[0018] In some embodiments of the present invention, the mass ratio of the Artemisia capillaris extract to the Rubus idaeus extract is 4-6:5-15.

[0019] In some embodiments of the present invention, the mass ratio of the Artemisia capillaris extract to the Rubus idaeus extract is 1:3.

[0020] In some embodiments of the present invention, the mass ratio of the Forsythia suspensa fruit extract, the Artemisia capillaris extract, and the Rubus idaeus extract is 5-15:4-6:5-15.

[0021] In some embodiments of the present invention, the mass ratio of the Forsythia suspensa fruit extract, the Artemisia capillaris extract, and the Rubus idaeus extract is 3:2:3.

[0022] In some embodiments of the present invention, the extraction process of the Forsythia suspensa fruit extract comprises the following steps:

[0023] The forsythia fruit is crushed, the forsythia fruit powder is extracted with a first solvent, ultrasonically extracted, and the extract is purified to obtain the forsythia fruit extract.

[0024] In some embodiments of the present invention, the first solvent comprises an ethanol aqueous solution with a mass concentration of 40-80%.

[0025] In some embodiments of the present invention, the mass ratio of the Forsythia suspensa fruit powder to the first solvent is 1:6-10.

[0026] In some embodiments of the present invention, after the Forsythia suspensa fruit is crushed, it is further sieved through a 60-100 mesh sieve.

[0027] In some embodiments of the present invention, the extraction temperature is 40-70° C. and the extraction time is 1-2 hours.

[0028] In some embodiments of the present invention, the ultrasonic extraction time is 1-3 hours.

[0029] In some embodiments of the present invention, the extraction process of the Artemisia capillaris extract comprises the following steps:

[0030] The Japanese cypress leaves are crushed, the Artemisia capillaris powder is extracted by ultrasonic extraction with a second solvent, and the extract is purified to obtain the Artemisia capillaris extract.

[0031] In some embodiments of the present invention, the second solvent comprises an ethanol aqueous solution with a mass concentration of 60-80%.

[0032] In some embodiments of the present invention, the mass ratio of the Artemisia capillaris extract to the second solvent is 1:6-10.

[0033] In some embodiments of the present invention, after the Artemisia capillaris is crushed, it is also sieved through a 60-100 mesh sieve.

[0034] In some embodiments of the present invention, the temperature of the ultrasonic extraction is 50-70° C. and the time is 2-6 hours.

[0035] In some embodiments of the present invention, the extraction process of the raspberry extract comprises the following steps:

[0036] The raspberries are crushed, the raspberry powder is subjected to ultrasonic extraction with a third solvent, and the extract is decolorized, concentrated and purified to obtain a raspberry extract.

[0037] In some embodiments of the present invention, the third solvent comprises an ethanol aqueous solution with a mass concentration of 50-80%.

[0038] In some embodiments of the present invention, the mass ratio of the raspberry powder to the third solvent is 1:6-10.

[0039] In some embodiments of the present invention, after the raspberries are crushed, they are also sieved through a 60-100 mesh sieve.

[0040] In some embodiments of the present invention, the extraction temperature is 50-70° C. and the extraction time is 2-6 hours.

[0041] Another object of the present invention is to provide the use of the plant-based composite antibacterial agent in the preparation of cosmetics, wherein the cosmetics contain the plant-based composite antibacterial agent, and the cosmetics include at least one of facial mask liquid, emulsion, cream, toner, essence, stock solution, facial cleanser, lotion, perfume, makeup remover, foundation liquid, foundation cream, concealer cream, rouge, lipstick, eye shadow, and blush.

[0042] In some embodiments of the present invention, the amount of the plant-derived composite antibacterial agent is 0.5-5% of the cosmetics in terms of mass percentage.

[0043] Compared with the prior art, the present invention has the following beneficial effects:

[0044] (1) The components of the plant-derived composite antibacterial agent provided by the present invention are all common plant extracts, which can replace traditional preservatives and have the advantages of being safe, mild, and non-irritating, and will not cause skin allergic reactions.

[0045] (2) The present invention can significantly improve the antibacterial and anti-inflammatory effects by giving full play to the synergistic effect between the extracts of the antibacterial agent, and can achieve the purpose of rapid and long-term antibacterial. The plant extract multi-effect composition can be used in cosmetics and has the advantages of low dosage, safety and effectiveness, and natural low irritation. DETAILED DESCRIPTION

[0046] In order to enable those skilled in the art to better understand the technical solutions of the present invention, the technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

[0047] Example 1

[0048] This embodiment provides an extraction process of Forsythia suspensa fruit extract, and the specific extraction process is as follows:

[0049] Take 55kg of forsythia fruit, crush it with a traditional Chinese medicine grinder, pass it through an 80-mesh sieve to obtain forsythia fruit powder, take 50kg of the sieved forsythia fruit powder, add 6-10 times the amount of 40-80% ethanol aqueous solution, mix evenly and then soak, generally soak for 1-2 hours, keep the temperature at about 55°C, after soaking, transfer the extraction mixture into an ultrasonic extraction device, use a high-power dual-frequency ultrasonic device to perform ultrasonic extraction for 2 hours, after the extraction is completed, filter the extract to remove impurities, and remove water and ethanol at an environment not higher than 60°C, and further ultrafilter the remaining forsythia fruit extract to remove impurities and purify, so as to obtain a forsythia fruit extract.

[0050] Example 2

[0051] This embodiment provides an extraction process of Artemisia capillaris extract, and the specific extraction process is as follows:

[0052] Take 55kg of Artemisia capillaris, grind it with a traditional Chinese medicine grinder, and then pass it through an 80-mesh sieve for later use. Take 50kg of the sieved Artemisia capillaris fine powder and add it to an extraction container, add 6-10 times the amount of 60-80% ethanol aqueous solution, and ultrasonically extract it 2-3 times at a temperature of 50-70°C and normal pressure, each time for 1-2h, combine the extracts, filter the extracts to remove impurities, and remove water and ethanol at an environment not higher than 60°C. The remaining Artemisia capillaris extract is further ultrafiltered to remove impurities and refined to obtain the Artemisia capillaris extract.

[0053] Example 3

[0054] This embodiment provides an extraction process of raspberry extract, and the specific extraction process is as follows:

[0055] 55 kg of raspberries are taken, crushed by a traditional Chinese medicine crusher, and then sieved through an 80-mesh sieve for later use; 50 kg of the sieved raspberry fine powder is taken and added to an extraction container; 6-10 times of a 50-80% ethanol aqueous solution is added; ultrasonic extraction is performed 2-3 times at a temperature of 50-70° C. and normal pressure, each time for 1-2 hours; the extracts are combined, 1-5% of activated carbon is added, the extracts are heated to reflux for decolorization for 0.5-2 hours, filtered while hot, and concentrated under reduced pressure at 65° C. to obtain a raspberry extract concentrate; the concentrate is adsorbed by a macroporous resin, eluted with 60-70% ethanol, and the eluate is spray-dried to obtain a raspberry extract.

[0056] Preparation of plant-derived composite antibacterial agent:

[0057] Table 1. Raw material composition of plant-derived composite antibacterial agent.

[0058] formula Raw materials and mass percentage Example 4 30% Forsythia suspensa fruit extract + 10% Artemisia capillaris extract + 60% 1,3-butylene glycol Example 5 15% Forsythia suspensa fruit extract + 25% Raspberry extract + 60% 1,3-Butanediol Example 6 10% Artemisia capillaris extract + 30% Raspberry extract 60% 1,3-Butanediol Example 7 15% Forsythia suspensa fruit extract + 10% Artemisia capillaris extract + 15% Raspberry extract + 60% 1,3-Butanediol Comparative Example 1 40% Forsythia suspensa fruit extract + 60% 1,3-butylene glycol Comparative Example 2 40% Artemisia capillaris extract + 60% 1,3-butylene glycol Comparative Example 3 40% Raspberry Extract + 60% 1,3-Butanediol

[0059] According to the raw materials and mass percentages in Table 1, the above extracts and 1,3-butanediol were added to a 250 mL flask equipped with a thermometer and a stirrer, the temperature was raised to 40-50°C, and stirred for 30-50 min until completely mixed. After cooling to room temperature, insoluble impurities were removed by filtration to obtain a plant-derived composite antibacterial agent.

[0060] Antiseptic efficacy test:

[0061] Test strains: The bacteria used in this experiment were a mixed strain of Pseudomonas aeruginosa ATCC9027, Escherichia coli ATCC8739 and Staphylococcus aureus ATCC6538; the fungi were Candida albicans ATCC10231 and Aspergillus niger ATCC16404, all of which were from Guangdong Provincial Microbiological Culture Collection Center.

[0062] The initial inoculum volume of the mixed bacteria used in the test was 10 5 ~10 6 CFU / g, the initial inoculum of Candida albicans and Aspergillus niger was 10 4 ~10 5 CFU / g, the bacterial content was determined on the 7th, 14th and 28th days, and the logarithmic value of the reduction in the number of various microorganisms at the corresponding time nodes was calculated. The preservatives in Tables 2 to 4 were the plant-based composite antibacterial agents of Examples 4 to 7 and Comparative Examples 1 to 3 in Table 1 respectively; the test systems were mask liquid, moisturizing lotion and repair cream. The formula and production process are shown in Tables 2 to 4.

[0063] Table 2. Facial mask liquid ingredients and production process.

[0064]

[0065] Table 3. Ingredients and production process of moisturizing lotion.

[0066]

[0067]

[0068] Table 4. Repair cream ingredients and production process.

[0069]

[0070]

[0071] The test results were judged as follows: the number of bacteria decreased by 3 orders of magnitude on the 7th day, and decreased by 3 orders of magnitude on the 14th and 28th days without increasing; the number of Candida albicans decreased by 1 order of magnitude on the 7th day, and decreased by one order of magnitude on the 14th and 28th days without increasing; the number of Aspergillus niger did not increase on the 14th day, and decreased by one order of magnitude on the 28th day, which was considered passed. The results are shown in Tables 5 to 7.

[0072] Table 5. Test data of antiseptic efficacy of facial mask liquid.

[0073]

[0074]

[0075] Table 6. Test data of the preservative efficacy of moisturizing lotions.

[0076]

[0077]

[0078] Table 7. Test data of the antiseptic efficacy of the repair cream.

[0079]

[0080] As shown in Tables 5-7, the plant-derived composite antibacterial agent prepared in Examples 4 to 7 passed the antiseptic efficacy test in the three test systems of mask liquid, moisturizing lotion, and repair cream, and has a strong antiseptic effect. The antibacterial spectrum and antibacterial ability of the three test systems of mask liquid, moisturizing lotion, and repair cream prepared by the single-component antibacterial agent in Comparative Examples 1 to 3 have defects in antiseptic efficacy and failed the antiseptic efficacy test. It can be seen that the plant-derived composite antibacterial agent has a synergistic antiseptic effect.

[0081] Test of anti-inflammatory ability:

[0082] The LPS-induced inflammatory model of mouse mononuclear macrophage leukemia cells is a classic cell model for studying inflammatory factors. The content of specific pro-inflammatory factors was determined by enzyme-linked immunosorbent assay (ELISA), and the difference in the secretion of pro-inflammatory factors by mouse mononuclear macrophage leukemia cells after administration of the negative control and the test substance was compared to evaluate the effect of the test substance in inhibiting the secretion of pro-inflammatory factors. Bacterial lipopolysaccharide (LPS) binds to the antigen recognition receptor on the surface of macrophages, inducing macrophages to secrete the pro-inflammatory factor IL-1β.

[0083] For specific methods, please refer to T / SHRH 033-2020 Cosmetic Soothing Efficacy Test-In Vitro IL-1β Inflammatory Factor Content Determination Lipopolysaccharide-induced Macrophage Inflammatory Cell Model Test Method.

[0084] Table 8. Test substance working solutions.

[0085]

[0086] After the test substance working solution is prepared, place it at 37℃±1℃, CO 2The cells were cultured for 24 hours at a concentration of 5% ± 1%, and then a sufficient amount of cell supernatant was collected and the inflammatory factor content was determined according to the instructions of the ELISA kit (ElisaKit CUSABIO). The positive control group inflammatory factor ≥ 25% proved that the test was effective. Finally, the inhibition rate was calculated according to formula (1):

[0087] Inhibition rate (%) = (1-T / C) × 100% Formula (1)

[0088] Where:

[0089] T—average value of the inflammatory factor content of the test substance.

[0090] C—Average value of inflammatory factor content in negative control.

[0091] Table 9. Anti-inflammatory ability test results.

[0092]

[0093]

[0094] From the results in Table 9, it can be seen that the anti-inflammatory effect of the combination of any two or more of Forsythia suspensa fruit extract, Artemisia capillaris extract, and Rubus idaeus extract is better than the effect of using any of the above components alone, among which the combination formed by the three extracts has the best anti-inflammatory effect, indicating that there is a synergistic anti-inflammatory effect between the above two or three components.

[0095] Human skin patch test:

[0096] The irritation and safety of the emulsions of Examples 12 to 15 containing the plant extract multi-effect compositions prepared in Examples 4 to 7 were tested. The formula and production process of the emulsions are shown in Table 3.

[0097] Test method: Refer to Chapter 7, Section 2, 6.1 Skin occluded patch test of the 2015 edition of the "Technical Specifications for Safety of Cosmetics". Weigh 0.02 mL of different emulsion products into the patch tester, and set up a blank control group at the same time. The control is the diluent of the cosmetic. 200 healthy volunteers were selected and divided into 4 groups, with 50 in each group, aged 18 to 24 years old, and no history of allergies. After cleaning the inner side of the subject's forearm, take 0.02 mL of sample and add it to the filter paper attached to the patch tester. Apply the patch tester to the inner side of the subject's forearm with non-irritating tape. After pasting, press it lightly with your fingers to make it evenly attached to the skin for 24 hours. Instruct the subject to keep the patch area dry within 24 hours, avoid intense exercise, scratching the patch test area, and long-term sunlight exposure. After 24 hours, remove the tester and mark it. Observe the skin reaction according to the standards in Table 9 at 30 minutes, 24 hours and 48 hours after removing the test patch tester, and record the observation results.

[0098] Table 10. Skin reaction grading standards for occlusive patch test

[0099]

[0100]

[0101] Table 11. Skin patch test results

[0102]

[0103] As can be seen from Table 11, the emulsions of Examples 12 to 15 containing the plant-derived composite antibacterial agents of Examples 4 to 7 had no serious adverse reactions, and the subjects basically had negative reactions. The results show that the emulsions of Examples 12 to 15 containing the plant-derived composite antibacterial agents prepared in Examples 4 to 7 have low irritation and high safety.

[0104] The mass percentage of the antibacterial agent in the plant-based composite antibacterial agent of the present invention is in the range of 20-60%, and the mass percentage of the solvent is in the range of 40-80% (such as: the mass percentage of the antibacterial agent is 20%, 25%, 30%, 35%, 45%, 50%, 55%, 60%, and the mass percentage of the solvent is 40%, 45%, 50%, 55%, 65%, 70%, 75%, 80%, etc.), and / or other solvents are used to extract Forsythia suspensa, Artemisia capillaris, and raspberry (such as: using ethanol, methanol, petroleum ether, ethyl acetate... and other solvents to extract Forsythia suspensa, Artemisia capillaris, and raspberry), and / or the plant-based composite antibacterial agent uses other solvents (such as 1,3-butanediol, 1,3-propylene glycol, 1,2-propylene glycol , 1,2-butylene glycol, 1,2-pentanediol, 1,5-pentanediol, isopentyl glycol, isohexanediol, 1,2-hexanediol, dipropylene glycol, water, glycerin, methylpropanediol, etc.), and / or the mass ratio of Forsythia suspensa fruit extract to Artemisia capillaris extract is 5-15:4-6, and / or the mass ratio of Forsythia suspensa fruit extract to Raspberry extract is 1-3:1-3, and / or the mass ratio of Artemisia capillaris extract to Raspberry extract is 4-6:5-15, and / or the mass ratio of Forsythia suspensa fruit extract, Artemisia capillaris extract and Raspberry extract is 5-15:4-6:5-15. The obtained plant-derived composite antibacterial agents all have synergistic preservative and synergistic anti-inflammatory effects among the antibacterial agents, and the plant-derived composite antibacterial agents are used to prepare cosmetics with low irritation and high safety.

[0105] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, ordinary technicians in the relevant field should understand that after reading the specification of this application, the technicians can still modify or replace the specific implementation mode of the present invention with equivalents, but these modifications or changes do not deviate from the scope of protection of the pending claims of the present application.

Claims

1. A plant-derived composite antibacterial agent, characterized in that: The raw materials include the following mass percentages: 20-60% of an antibacterial agent, wherein the antibacterial agent includes a mixture of at least two of a Forsythia suspensa fruit extract, an Artemisia capillaris extract, and a raspberry extract; The mass ratio of the Forsythia suspensa fruit extract to the Artemisia capillaris extract is 5-15:4-6; The mass ratio of the forsythia fruit extract to the raspberry extract is 1-3:1-3; The mass ratio of the Artemisia capillaris extract to the raspberry extract is 4-6:5-15; The extraction process of the forsythia fruit extract comprises the following steps: The forsythia fruit is crushed, the forsythia fruit powder is extracted with a first solvent, ultrasonically extracted, and the extract is purified to obtain a forsythia fruit extract; The extraction process of the Artemisia capillaris extract comprises the following steps: crushing Artemisia capillaris, extracting the Artemisia capillaris powder with a second solvent by ultrasonic wave, purifying the extract, and obtaining an Artemisia capillaris extract; The extraction process of the raspberry extract comprises the following steps: The raspberries are crushed, the raspberry powder is subjected to ultrasonic extraction with a third solvent, and the extract is decolorized, concentrated, and purified to obtain a raspberry extract; The first solvent comprises an ethanol aqueous solution with a mass concentration of 40-80%; The second solvent comprises an ethanol aqueous solution with a mass concentration of 60-80%; The third solvent comprises an ethanol aqueous solution with a mass concentration of 50-80%.

2. The plant-derived composite antibacterial agent according to claim 1, characterized in that: Also includes 40-80% solvent.

3. The plant-derived composite antibacterial agent according to claim 2, characterized in that: The solvent includes at least one of 1,3-butanediol, 1,3-propylene glycol, 1,2-propylene glycol, 1,2-butanediol, 1,4-butanediol, 1,2-pentanediol, 1,5-pentanediol, isoprene glycol, isohexanediol, 1,2-hexanediol, dipropylene glycol, water, glycerol, and methylpropylene glycol.

4. The plant-derived composite antibacterial agent according to claim 1, characterized in that: The mass ratio of the Forsythia suspensa fruit extract, the Artemisia capillaris extract and the Rubus idaeus extract is 5-15:4-6:5-15.

5. The plant-derived composite antibacterial agent according to claim 1, characterized in that: The mass ratio of the forsythia fruit powder to the first solvent is 1:6-10; The mass ratio of the Artemisia capillaris powder to the second solvent is 1:6-10; The mass ratio of the raspberry powder to the third solvent is 1:6-10.

6. The use of the plant-derived composite antibacterial agent according to any one of claims 1 to 5 in the preparation of cosmetics, characterized in that: The cosmetics contain the plant-derived composite antibacterial agent, and the cosmetics include at least one of lotion, cream, toner, stock solution, cleanser, lotion, perfume, rouge, lipstick, eye shadow, and blush.

7. The use of the plant-derived composite antibacterial agent according to any one of claims 1 to 5 in the preparation of cosmetics, characterized in that: The cosmetics contain the plant-derived composite antibacterial agent, and the cosmetics include at least one of a facial mask liquid, an essence liquid, a makeup remover, a foundation liquid, a foundation cream, and a concealer cream.

8. The use according to claim 6, characterized in that Calculated by mass percentage, the amount of the plant-derived composite antibacterial agent used is 0.5-5% of the cosmetics.

9. The use according to claim 7, characterized in that Calculated by mass percentage, the amount of the plant-derived composite antibacterial agent used is 0.5-5% of the cosmetics.

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