Composite herbal plant composition for removing dandruff, preventing depigmentation and relieving itching, fermentation process and application thereof

Abstract

The present invention relates to the technical field of toiletries, and specifically discloses a composite herbal plant composition for dandruff, anti-hair loss and anti-itching, a fermentation process and its application. The fermentation process includes: 1), damaging Sophora flavescens, Platycladus orientalis, Angelica sinensis, Gentiana macrophylla, Cnidium monnieri and Viola yedoensis to obtain composite plant particles; 2), putting the composite plant particles into water, heating to boiling, condensing and refluxing, extracting for 2-3 hours, and obtaining a pre-extract; 3), cooling the pre-extract to room temperature, inoculating a composite bacteria composed of Schizosaccharomyces pombe, Pichia pastoris and Succinic acid-producing filamentous bacillus, fermenting for 96-120 hours, and obtaining a fermentation extract; 4), adding ethanol to the fermentation extract, heating to 68-72°C, extracting for 90-120 minutes, cooling, filtering, obtaining a filtrate, removing impurities, and obtaining a composite herbal plant composition for dandruff, anti-hair loss and anti-itching. The present invention has the advantage of having a good anti-hair loss effect for patients with androgenic alopecia.

Description

Technical Field

[0001] The invention relates to the field of toiletries, and in particular to a composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching, a fermentation process and an application thereof. Background Art

[0002] Hair is the hair that grows on the head and has the function of protecting the head. With the development of society, people's pursuit of beauty has reached a higher level. In addition to its original physiological function, hair can also enhance people's spirit through various unique shapes. Therefore, people pay more and more attention to hair care.

[0003] Hair loss refers to the phenomenon of hair falling out. Normally, the hair that falls out is in the regression phase and the resting phase. Since the hair entering the regression phase and the hair entering the new growth phase are constantly in a dynamic balance, the normal amount of hair can be maintained. However, many people are currently facing the trouble of pathological hair loss. The essence of pathological hair loss is actually that the speed of hair loss is greater than the speed of hair growth, resulting in a decrease in the amount of hair, which in turn brings about problems such as sparse hair. In order to overcome the problem of hair loss, existing toiletries usually add ingredients that promote hair growth to slow down the problem of hair loss by promoting hair growth.

[0004] However, there are many causes of pathological hair loss, and androgenic alopecia is one of them. It is a type of persistent hair loss. Although existing anti-hair loss care products can supplement nutrition for hair and promote hair growth, they usually lack the effect of inhibiting DHT production, resulting in a relatively general anti-hair loss effect for patients with androgenic alopecia. Therefore, there is still room for improvement. Summary of the invention

[0005] In order to achieve a better anti-hair loss effect for patients with androgenic alopecia, the present application provides a composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching, as well as a fermentation process and application thereof.

[0006] In the first aspect, the present application provides a composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching, which adopts the following technical scheme:

[0007] A fermentation process of a composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching comprises the following steps:

[0008] Step 1), breaking Sophora flavescens, Platycladus orientalis leaves, Angelica sinensis, Gentiana macrophylla, Cnidium monnieri, and Viola yedoensis to obtain composite plant particles;

[0009] Step 2), putting the composite plant particles into water, heating to boiling, condensing and refluxing, extracting for 2-3 hours, and obtaining a pre-extract;

[0010] Step 3), cooling the pre-extract to room temperature, inoculating a composite bacteria composed of Schizosaccharomyces pombe, Pichia pastoris, and Bacillus succinogenes, and fermenting for 96-120 hours to obtain a fermentation extract;

[0011] Step 4), adding ethanol to the fermented extract, heating to 68-72° C., extracting for 90-120 min, cooling, filtering, obtaining a filtrate, removing impurities, and obtaining a composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching;

[0012] In the step 1), the mass ratio of Sophora flavescens, Platycladus orientalis leaves, Angelica sinensis, Gentiana macrophylla, Cnidium monnieri, and Viola yedoensis is 4.5-5.5:0.5-1.5:1.5-2.5:1.5-2.5:1.5-2.5:0.5-1.5;

[0013] In the step 3), the mass ratio of Schizosaccharomyces pombe, Pichia pastoris, and Bacillus succinigensis is 0.0002-0.0004: 0.0005-0.0007: 0.0004-0.0006.

[0014] By adopting the above technical scheme, by compounding and extracting Sophora flavescens, Platycladus orientalis, Angelica sinensis, Gentiana macrophylla, Cnidium monnieri, and Viola yedoensis, the active ingredients in Sophora flavescens, Platycladus orientalis, Angelica sinensis, Gentiana macrophylla, Cnidium monnieri, and Viola yedoensis can cooperate well with each other, and by fermenting with a composite bacteria composed of Schizosaccharomyces pombe, Pichia powdery yeast, and Bacillus succinogenes, the cell walls of the plants can be well destroyed, so that the effective ingredients in the composite plants can be better extracted, and the content of the active ingredients in the composition is higher. Moreover, the active ingredients are fermented by the composite bacteria to form a small molecule structure that is easier to absorb. The active ingredients after fermentation can significantly inhibit the formation of dihydrotestosterone (DHT), have a good anti-hair loss effect for patients with androgenic alopecia, and can effectively reduce irritating ingredients, making the composition more gentle, reducing the negative impact of irritating substances on the anti-dandruff, anti-hair loss and anti-itching effects of the composition, so that the composition can play a good anti-dandruff, anti-hair loss and anti-itching effect after being added to toiletries.

[0015] Preferably, the mass ratio of the composite plant particles to the composite bacteria is 10-16:0.0011-0.0017.

[0016] By adopting the above technical solution, the fermentation effect of the composite bacteria is better, while the waste of the composite bacteria is reduced, and the economic value is higher.

[0017] Preferably, the mass ratio of the composite plant particles to the water is 4-5:2-3, and the mass ratio of the water to the ethanol is 4-5:2-3.

[0018] By adopting the above technical scheme and specifically selecting the ratio of water to ethanol, the solubility of the solvent is improved, the extraction effect of the active ingredient is better, and the anti-dandruff, anti-loss and anti-itching effects of the prepared composition are better.

[0019] Preferably, in the step 4), after adding ethanol, carbon dioxide is continuously introduced until the heating process is completed, and 100-200 mL / h of carbon dioxide is introduced per 1 kg of water.

[0020] By adopting the above technical solution and introducing carbon dioxide, the pH value in the water is made more suitable for the growth of the composite bacteria, the activity of the composite bacteria is effectively improved, the fermentation effect is better, and thus the anti-dandruff, anti-loss and anti-itching effects of the composition are better.

[0021] Preferably, in step 4), when removing impurities, D113 resin and RS407 resin are added to the filtrate, stirred for 30-40 minutes, and filtered to complete the impurity removal.

[0022] By adopting the above technical solution, by adding D113 resin and RS407 resin into the filtrate, impurities are selectively removed, the purity of the effective active ingredients in the composition is higher, and the anti-dandruff, anti-loss and anti-itching effects of the composition are better.

[0023] Preferably, the mass ratio of the filtrate, D113 resin and RS407 resin is 10:1.3-1.7:1.3-1.7.

[0024] By adopting the above technical scheme and specifically selecting the mass ratio of the filtrate, D113 resin and RS407 resin, the impurity removal effect is better, the active ingredients in the composition are purer, and the anti-dandruff, anti-loss and anti-itching effects are better.

[0025] In a second aspect, the present application provides a method for preparing a composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching, using the following technical scheme:

[0026] A composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching is prepared by a fermentation process of the composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching.

[0027] By adopting the above technical scheme, the composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching has the effect of significantly inhibiting the production of DHT, has a good hair loss prevention effect for patients with androgenic alopecia, and has better efficacy in removing dandruff, preventing hair loss and relieving itching.

[0028] In a third aspect, the present application provides an application of a composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching, using the following technical solution:

[0029] An application of the above-mentioned composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching, wherein the composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching is added to toiletries to prepare toiletries with the effects of removing dandruff, preventing hair loss and relieving itching, and the mass percentage of the composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching in the toiletries is 10-15%.

[0030] By adopting the above technical scheme, the washing and care products added with the composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching have good effects of removing dandruff, preventing hair loss and relieving itching, especially for patients with androgenic alopecia, have good anti-hair loss effect.

[0031] In summary, this application has the following beneficial effects:

[0032] 1. The present application extracts by compounding Sophora flavescens, Platycladus orientalis, Angelica sinensis, Gentiana macrophylla, Cnidium monnieri, and Viola yedoensis, so that the active ingredients in Sophora flavescens, Platycladus orientalis, Angelica sinensis, Gentiana macrophylla, Cnidium monnieri, and Viola yedoensis can cooperate well with each other, and the composite bacteria formed by compounding Schizosaccharomyces pombe, Pichia powdery yeast, and succinic acid-producing filamentous bacillus are used for fermentation, which can well destroy the cell walls of the plants, so that the effective ingredients in the composite plants can be better extracted, and the content of active ingredients in the composition is higher. Moreover, the active ingredients are fermented by the composite bacteria to form a small molecule structure that is easier to absorb. The active ingredients after fermentation can significantly inhibit the formation of dihydrotestosterone (DHT), have a good anti-hair loss effect for patients with androgenic alopecia, and can effectively reduce irritating ingredients, making the composition more mild, reducing the negative impact of irritating substances on the anti-dandruff, anti-hair loss and anti-itching effects of the composition, so that the composition can play a good anti-dandruff, anti-hair loss and anti-itching effect after being added to toiletries.

[0033] 2. In the present application, it is preferred to specifically select the ratio of water to ethanol so that the solubility of the solvent is better, the extraction effect of the active ingredient is better, and the anti-dandruff, anti-loss and anti-itching effects of the prepared composition are better.

[0034] 3. In the present application, it is preferred to selectively remove impurities by adding D113 resin and RS407 resin to the filtrate, so that the purity of the effective active ingredients in the composition is higher, so that the anti-dandruff, anti-loss and anti-itching effects of the composition are better. DETAILED DESCRIPTION

[0035] The present application is further described in detail below with reference to the embodiments.

[0036] Example 1

[0037] A fermentation process of a composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching comprises the following steps:

[0038] Step 1), 4.5 kg of Sophora flavescens, 0.5 kg of Platycladus orientalis leaves, 1.5 kg of Angelica sinensis, 1.5 kg of Gentiana macrophylla, 1.5 kg of Cnidium monnieri, and 0.5 kg of Viola yedoensis are broken into particles, and passed through a 10-mesh sieve to obtain composite plant particles.

[0039] Step 2), 10 kg of composite plant particles and 40 kg of water are put into a reaction kettle, heated to boiling, condensed and refluxed, and extracted for 2 hours to obtain a pre-extract.

[0040] Step 3), the pre-extract is cooled to room temperature, inoculated with a composite bacteria composed of 0.0002 kg of Schizosaccharomyces pombe, 0.0005 kg of powdered Pichia pastoris, and 0.0004 kg of Bacillus succinogenes, and fermented at a constant temperature of 32° C. for 96 hours to obtain a fermentation extract.

[0041] Step 4), add 20kg of ethanol to the fermentation extract, introduce carbon dioxide, the amount of carbon dioxide introduced is 4000mL / h, heat to 68°C, extract for 90min, cool to room temperature, filter through filter cloth, discard the filter residue, obtain filtrate, and concentrate the filtrate by rotary evaporation, the rotary evaporation temperature is 60°C, the rotary evaporation pressure is 0.01Mpa, the mass of the concentrated filtrate is 10kg, and then 1.3kgD113 resin and 1.3kgRS407 resin are added to the filtrate, the speed is 20r / min, and the stirring is carried out for 30-40min. Filter through filter cloth, discard the filter residue, take the filtrate, and obtain a composite herbal plant composition for anti-dandruff, anti-loss and antipruritic.

[0042] Sophora flavescens, Platycladus orientalis leaves, Chinese angelica, Gentiana macrophylla, Cnidium monnieri and Viola yedoensis are all commercially available and are sun-dried Chinese medicinal materials.

[0043] Water is deionized water, homemade.

[0044] Ethanol was purchased from Jinan Yuyi Chemical Co., Ltd., anhydrous ethanol, CAS number: 64-17-5.

[0045] Schizosaccharomyces pombe was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-58027.

[0046] Powdered Pichia yeast was purchased from Beijing Bio-Bowei Biotechnology Co., Ltd., platform number: Bio-29310.

[0047] Succinic acid-producing Bacillus was purchased from Beijing Bio-Bowei Biotechnology Co., Ltd., platform number: Bio-091322.

[0048] D113 resin was purchased from Shanghai Jinzhu Resin Co., Ltd.

[0049] RS407 resin was purchased from Anhui Rongsheng Environmental Protection Water Treatment Co., Ltd.

[0050] Example 2

[0051] A fermentation process of a composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching comprises the following steps:

[0052] Step 1), 5kg of Sophora flavescens, 1kg of Platycladus orientalis leaves, 2kg of Angelica sinensis, 2kg of Gentiana macrophylla, 2kg of Cnidium monnieri, and 1kg of Viola yedoensis are broken into particles, and the particles are passed through a 14-mesh sieve to obtain composite plant particles.

[0053] Step 2), 13 kg of composite plant particles and 45 kg of water are put into a reaction kettle, heated to boiling, condensed and refluxed, and extracted for 2.5 hours to obtain a pre-extract.

[0054] Step 3), the pre-extract is cooled to room temperature, inoculated with a composite bacteria composed of 0.0003 kg of Schizosaccharomyces pombe, 0.0006 kg of powdered Pichia pastoris, and 0.0005 kg of Bacillus succinogenes, and fermented for 108 hours at a constant temperature of 32.5°C to obtain a fermentation extract.

[0055] Step 4), add 25kg of ethanol to the fermentation extract, introduce carbon dioxide, the amount of carbon dioxide introduced is 7000mL / h, heat to 70°C, extract for 105min, cool to room temperature, filter through filter cloth, discard the filter residue, obtain filtrate, and concentrate the filtrate by rotary evaporation, the rotary evaporation temperature is 60°C, the rotary evaporation pressure is 0.01Mpa, the mass of the concentrated filtrate is 13kg, and then 1.5kgD113 resin and 1.5kgRS407 resin are added to the filtrate, the speed is 20r / min, and the stirring is carried out for 30-40min. Filter through filter cloth, discard the filter residue, take the filtrate, and obtain a composite herbal plant composition for anti-dandruff, anti-loss and antipruritic.

[0056] Sophora flavescens, Platycladus orientalis leaves, Chinese angelica, Gentiana macrophylla, Cnidium monnieri and Viola yedoensis are all commercially available and are sun-dried Chinese medicinal materials.

[0057] Water is deionized water, homemade.

[0058] Ethanol was purchased from Jinan Yuyi Chemical Co., Ltd., anhydrous ethanol, CAS number: 64-17-5.

[0059] Schizosaccharomyces pombe was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-58027.

[0060] Powdered Pichia yeast was purchased from Beijing Bio-Bowei Biotechnology Co., Ltd., platform number: Bio-29310.

[0061] Succinic acid-producing Bacillus was purchased from Beijing Bio-Bowei Biotechnology Co., Ltd., platform number: Bio-091322.

[0062] D113 resin was purchased from Shanghai Jinzhu Resin Co., Ltd.

[0063] RS407 resin was purchased from Anhui Rongsheng Environmental Protection Water Treatment Co., Ltd.

[0064] Example 3

[0065] A fermentation process of a composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching comprises the following steps:

[0066] Step 1), 5.5 kg of Sophora flavescens, 1.5 kg of Platycladus orientalis leaves, 2.5 kg of Angelica sinensis, 2.5 kg of Gentiana macrophylla, 2.5 kg of Cnidium monnieri, and 1.5 kg of Viola yedoensis are broken into particles, and passed through a 20-mesh sieve to obtain composite plant particles.

[0067] Step 2), 16 kg of composite plant particles and 50 kg of water are put into a reaction kettle, heated to boiling, condensed and refluxed, and extracted for 3 hours to obtain a pre-extract.

[0068] Step 3), the pre-extract is cooled to room temperature, inoculated with a composite bacteria composed of 0.0004 kg of Schizosaccharomyces pombe, 0.0007 kg of powdered Pichia pastoris, and 0.0006 kg of Bacillus succinogenes, and fermented at a constant temperature of 33° C. for 120 hours to obtain a fermentation extract.

[0069] Step 4), add 30kg of ethanol to the fermentation extract, introduce carbon dioxide, the amount of carbon dioxide introduced is 10000mL / h, heat to 72°C, extract for 120min, cool to room temperature, filter through filter cloth, discard the filter residue, obtain filtrate, and concentrate the filtrate by rotary evaporation, the rotary evaporation temperature is 60°C, the rotary evaporation pressure is 0.01Mpa, the mass of the concentrated filtrate is 16kg, and then 1.7kgD113 resin and 1.7kgRS407 resin are added to the filtrate, the speed is 20r / min, stirred for 30-40min, filtered through filter cloth, discarded the filter residue, and the filtrate is taken to obtain a composite herbal plant composition for anti-dandruff, anti-loss and antipruritic.

[0070] Sophora flavescens, Platycladus orientalis leaves, Chinese angelica, Gentiana macrophylla, Cnidium monnieri and Viola yedoensis are all commercially available and are sun-dried Chinese medicinal materials.

[0071] Water is deionized water, homemade.

[0072] Ethanol was purchased from Jinan Yuyi Chemical Co., Ltd., anhydrous ethanol, CAS number: 64-17-5.

[0073] Schizosaccharomyces pombe was purchased from Beijing Biobio Biotech Co., Ltd., platform number: Bio-58027.

[0074] Powdered Pichia yeast was purchased from Beijing Bio-Bowei Biotechnology Co., Ltd., platform number: Bio-29310.

[0075] Succinic acid-producing Bacillus was purchased from Beijing Bio-Bowei Biotechnology Co., Ltd., platform number: Bio-091322.

[0076] D113 resin was purchased from Shanghai Jinzhu Resin Co., Ltd.

[0077] RS407 resin was purchased from Anhui Rongsheng Environmental Protection Water Treatment Co., Ltd.

[0078] Comparative Example 1

[0079] The fermentation process of a composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching is different from that of Example 2 only in that:

[0080] In step 3), an equal amount of Saccharomyces cerevisiae is used to replace Schizosaccharomyces pombe.

[0081] Saccharomyces cerevisiae was purchased from Beijing Biobio Biotechnology Co., Ltd., platform number: Bio-52554.

[0082] Comparative Example 2

[0083] The fermentation process of a composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching is different from that of Example 2 only in that:

[0084] In step 3), an equal amount of Pichia pastoris was used to replace the powdered Pichia pastoris.

[0085] Yeast was purchased from Beijing Bio-Bowei Biotechnology Co., Ltd., platform number: Bio-07482.

[0086] Comparative Example 3

[0087] The fermentation process of a composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching is different from that of Example 2 only in that:

[0088] In step 3), an equal amount of Butyrivibrio fibrinolyticus is used to replace Bacillus succinigenes.

[0089] Butyrivibrio fibrinolyticus was placed in Beijing Biobio Biotech Co., Ltd., platform number: Bio-117967.

[0090] Comparative Example 4

[0091] The fermentation process of a composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching is different from that of Example 2 only in that:

[0092] In step 3), an equal amount of Saccharomyces cerevisiae is used to replace Schizosaccharomyces pombe; an equal amount of Pichia pastoris is used to replace Pichia pastoris; and an equal amount of Butyrivibrio fibrinolyticus is used to replace Bacillus succinogenes.

[0093] Saccharomyces cerevisiae was purchased from Beijing Biobio Biotechnology Co., Ltd., platform number: Bio-52554.

[0094] Yeast was purchased from Beijing Bio-Bowei Biotechnology Co., Ltd., platform number: Bio-07482.

[0095] Butyrivibrio fibrinolyticus was placed in Beijing Biobio Biotech Co., Ltd., platform number: Bio-117967.

[0096] Comparative Example 5

[0097] The fermentation process of a composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching is different from that of Example 2 only in that:

[0098] In step 4), D318 resin was used to replace D113 resin in equal amounts.

[0099] D318 resin was purchased from Shanghai Kaiping Resin Co., Ltd.

[0100] Comparative Example 6

[0101] The fermentation process of a composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching is different from that of Example 2 only in that:

[0102] In step 4), D-101 resin is used to replace RS407 resin in equal amount.

[0103] D-101 resin was purchased from Hebei Lijiang Biotechnology Co., Ltd.

[0104] Comparative Example 7

[0105] The fermentation process of a composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching is different from that of Example 2 only in that:

[0106] In step 4), D318 resin is used to replace D113 resin in equal amounts; D-101 resin is used to replace RS407 resin in equal amounts.

[0107] D318 resin was purchased from Shanghai Kaiping Resin Co., Ltd.

[0108] D-101 resin was purchased from Hebei Lijiang Biotechnology Co., Ltd.

[0109] Experiment 1

[0110] Anti-dandruff and anti-itching experiment:

[0111] Prepare the shampoo to be tested. The preparation method of the shampoo to be tested is as follows:

[0112] Weigh 1.5 kg of cocamide MEA, 5 kg of cocamidopropyl betaine, 6 kg of glycerin, 12 kg of sodium laureth sulfate, 0.5 kg of guar hydroxypropyltrimethylammonium chloride, 1 kg of dehydrogenated glucoside, 0.5 kg of polydimethylsiloxane, 13 kg of the composite herbal plant composition for anti-dandruff, anti-hair loss and antipruritic effects, 0.2 kg of sclerotium gum, 0.1 kg of disodium EDTA and 60.2 kg of deionized water, put them into a stirring kettle, rotate at 60 r / min, stir for 15 min, mix evenly, and obtain the shampoo to be tested.

[0113] The anti-dandruff, anti-hair loss and anti-itching composite herbal plant composition is the anti-dandruff, anti-hair loss and anti-itching composite herbal plant composition of each embodiment and comparative example.

[0114] A number of volunteers were recruited and divided into groups to use the shampoo to be tested to which the composite herbal plant composition for preventing dandruff, hair loss and relieving itching of each embodiment or comparative example was added. Each group of 10 volunteers washed their hair with the shampoo to be tested once every 4 days. The amount of dandruff was tested and whether the scalp was itchy was recorded before each shampooing. The test was conducted 5 times in total.

[0115] The method for testing the amount of dandruff is as follows: during the test, separate the hair to both sides along the midline of the top of the head to form a hair dividing line, stick the dandruff test card on the hair dividing line for 5 seconds, remove the dandruff test card, record the amount of dandruff adhered to the test card, and take the average of the experimental data of each group.

[0116] The experimental data of Experiment 1 are detailed in Tables 1 and 2.

[0117] Table 1

[0118]

[0119]

[0120] Table 2

[0121]

[0122] According to the data comparison in Tables 1-2, each embodiment can achieve a good effect of inhibiting dandruff after washing the hair three times. The amount of dandruff is also less if the hair is not washed for four days. The anti-dandruff effect of each embodiment is significantly higher than that of each comparative example. It can be seen that after each embodiment uses a composite bacteria composed of Schizosaccharomyces pombe, Pichia powdery yeast, and Succinic acid-producing Bacillus to ferment the composite plant particles composed of Sophora flavescens, Platycladus orientalis, Angelica sinensis, Gentiana macrophylla, Cnidium monnieri, and Viola yedoensis, the obtained composite herbal plant composition for anti-dandruff, anti-hair loss and antipruritic effect is better.

[0123] Comparative Examples 1-4 destroyed the composite system of Schizosaccharomyces pombe, Pichia pastoris, and Bacillus succinogenes, thus changing the properties of the fermentation products, resulting in a significant decrease in the anti-dandruff and antipruritic effects of the prepared composition.

[0124] In Comparative Example 5-7, since the compounding system of D113 resin and RS407 resin was destroyed, the specific impurity removal effect was reduced, and the anti-dandruff and antipruritic effect of the obtained composite herbal plant composition was reduced.

[0125] Experiment 2

[0126] 5α-reductase inhibition assay:

[0127] 1. Experimental Principle

[0128] Androgenic alopecia (AGA) is caused by the conversion of testosterone (T) into more active dihydrotestosterone (DHT) under the action of 5α-reductase, which is sensitive to hair follicles, affects hair follicle development, and causes hair loss. By analyzing whether the test substance can inhibit 5α-reductase and thus inhibit the production of DHT, it can be used as one of the bases for judging whether it has the effect of preventing hair loss and promoting hair growth.

[0129] 2. Experimental Materials

[0130] 2.1 Type II 5α-reductase: extracted from rat prostate tissue and stored at -80°C.

[0131] 2.2 Positive control (PC): 1 μM finasteride solution.

[0132] 2.3 Sample preparation (TA): The anti-dandruff, anti-hair loss and anti-itching composite herbal plant compositions of each embodiment and comparative example were diluted to 5% (v / v) using PBS buffer solution.

[0133] 2.4 Blank control: phosphate buffered saline (PBS)

[0134] 3. Experimental steps

[0135] 3.1 Preparation of type II 5α-reductase: Take 10 male rats, fast overnight but not water, and then kill them by dislocating the cervical vertebrae. Take out the prostate and cut it into pieces on an ice table. Make a 1:5 homogenate in a glass homogenizer with pre-cooled buffer at 0℃, centrifuge at 3000g for 10min, take the supernatant and centrifuge at 10000g for 30min, take the supernatant and dispense it into EP tubes, 1mL per tube, which is the enzyme extract, and store it in a -80℃ refrigerator.

[0136] 3.2 Type II 5α-reductase activity assay: A 2 mL reaction system contained: 0.25 mL of phosphate buffer, 0.70 mL of enzyme extract, 0.1 mL of testosterone solution (0.5 mg / mL), 0.35 mL of NADPH solution (1 mg / mL), (10% ethanol in the complete reaction group, finasteride in the positive control group, and sample solution in the sample group) 0.6 mL. The control group replaced the enzyme extract with phosphate buffer and added 2.0 mL of dichloromethane to terminate the reaction; the complete reaction group, the positive group, and the sample group reacted at 37°C for 30 min, and after the reaction, 2.0 mL of dichloromethane was added to stop the reaction, and the reaction was shaken for 1 min and centrifuged at 5000 r / min for 10 min. The upper aqueous phase was removed, about 1.0 mL of the organic phase was removed and evaporated to dryness, the residue was dissolved in 1.0 mL of methanol, and the testosterone peak area (T) was determined by high performance liquid chromatography. Three parallel tubes were set for each reaction.

[0137] Blank control group: In the absence of type II 5α-reductase, testosterone cannot be converted into dihydrotestosterone, and the final measurement is the content of testosterone itself.

[0138] Complete reaction group: Testosterone (T) is converted into dihydrotestosterone under the action of 5α-reductase, and the final measurement is the content of remaining testosterone after the reaction.

[0139] Positive group / sample group: The positive substance / sample inhibits the conversion of testosterone (T) to dihydrotestosterone by inhibiting the activity of type II 5α-reductase, and finally determines the content of remaining testosterone after the reaction. The inhibition rate is calculated and statistically analyzed to determine whether the activity of type II 5α-reductase is effectively inhibited.

[0140] 3.3 Result analysis:

[0141]

[0142] The test data of Experiment 2 are detailed in Table 3.

[0143] Table 3

[0144] Inhibition rate of positive group (%) Sample group inhibition rate (%) Example 1 74.85 62.92 Example 2 74.91 63.36 Example 3 74.96 63.17 Comparative Example 1 74.89 41.65 Comparative Example 2 74.88 45.26 Comparative Example 3 74.92 43.68 Comparative Example 4 74.83 40.07 Comparative Example 5 74.95 58.79 Comparative Example 6 74.81 59.86 Comparative Example 7 74.93 57.54

[0145] According to the data comparison in Table 3, the inhibition rate of each embodiment is close to that of the positive group, and is significantly higher than that of each comparative example. It can be seen that in each embodiment, the composite plant composed of Sophora flavescens, Platycladus orientalis, Angelica sinensis, Gentiana macrophylla, Cnidium monnieri and Viola yedoensis is fermented by the composite bacteria composed of Schizosaccharomyces pombe, Pichia powdery yeast and Bacillus succinogenes, and the prepared composition has a good effect of inhibiting the production of DHT, and the composition has a good anti-hair loss effect for patients with androgenic alopecia.

[0146] Although each comparative example also uses a composite plant composed of Sophora flavescens, Platycladus orientalis leaves, Angelica sinensis, Gentiana macrophylla, Cnidium monnieri, and Viola yedoensis, the fermentation effect is significantly changed due to the destruction of the composite system of Schizosaccharomyces pombe, Pichia powdery yeast, and Bacillus succinogenes. The fermentation product cannot achieve the effect of significantly inhibiting the production of DHT. Therefore, the anti-hair loss effect for patients with androgenic alopecia is significantly reduced.

[0147] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A fermentation process of a composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching, characterized in that: The following steps are involved: Step 1), breaking Sophora flavescens, Platycladus orientalis leaves, Angelica sinensis, Gentiana macrophylla, Cnidium monnieri, and Viola yedoensis to obtain composite plant particles; Step 2), putting the composite plant particles into water, heating to boiling, condensing and refluxing, extracting for 2-3 hours, and obtaining a pre-extract; Step 3), cooling the pre-extract to room temperature, inoculating a composite bacteria composed of Schizosaccharomyces pombe, Pichia pastoris, and Bacillus succinogenes, and fermenting for 96-120 hours to obtain a fermentation extract; Step 4), adding ethanol to the fermented extract, heating to 68-72°C, extracting for 90-120 minutes, cooling, filtering, obtaining a filtrate, removing impurities, and obtaining a composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching; In the step 1), the mass ratio of Sophora flavescens, Platycladus orientalis leaves, Angelica sinensis, Gentiana macrophylla, Cnidium monnieri, and Viola yedoensis is 4.5-5.5:0.5-1.5:1.5-2.5:1.5-2.5:1.5-2.5:0.5-1.5; In the step 3), the mass ratio of Schizosaccharomyces pombe, Pichia pastoris, and Bacillus succinogenes is 0.0002-0.0004: 0.0005-0.0007: 0.0004-0.0006; The Schizosaccharomyces pombe was purchased from Beijing Bio-Bowei Biotechnology Co., Ltd., platform number: Bio-58027; The powdered Pichia yeast was purchased from Beijing Bio-Bowei Biotechnology Co., Ltd., platform number: Bio-29310; The succinic acid-producing filamentous bacteria were purchased from Beijing Biobo Biotechnology Co., Ltd., platform number: Bio-091322.

2. The fermentation process of the composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching according to claim 1, characterized in that: The mass ratio of the composite plant particles to the composite bacteria is 10-16:0.0011-0.0017.

3. The fermentation process of the composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching according to claim 1, characterized in that: The mass ratio of the composite plant particles to the water is 4-5:2-3, and the mass ratio of the water to the ethanol is 4-5:2-3.

4. The fermentation process of the composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching according to claim 1, characterized in that: In the step 4), after adding ethanol, carbon dioxide is continuously introduced until the heating process is completed, and 100-200 mL / h of carbon dioxide is introduced per 1 kg of water.

5. The fermentation process of the composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching according to claim 1, characterized in that: In the step 4), when removing impurities, D113 resin and RS407 resin are added to the filtrate, stirred for 30-40 minutes, and filtered to complete the impurity removal. The RS407 resin was purchased from Anhui Rongsheng Environmental Protection Water Treatment Co., Ltd.

6. The fermentation process of the composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching according to claim 5, characterized in that: The mass ratio of the filtrate, D113 resin and RS407 resin is 10: 1.3-1.7：1.3-1.7。 7. A composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching, characterized in that: The invention is prepared by the fermentation process of the anti-dandruff, anti-shedding and antipruritic composite herbal plant composition according to any one of claims 1 to 6.

8. An application of the composite herbal plant composition for removing dandruff, preventing hair loss and relieving itching according to claim 7, characterized in that: The anti-dandruff, anti-hair loss and anti-itching composite herbal plant composition is added to toiletries to prepare toiletries with anti-dandruff, anti-hair loss and anti-itching effects. The mass percentage of the anti-dandruff, anti-hair loss and anti-itching composite herbal plant composition in the toiletries is 10-15%.

Images