Antibacterial antiperspirant cream containing agarwood

Abstract

The invention discloses an antibacterial and antiperspirant cream containing agarwood, which achieves the antibacterial and antiperspirant effect by compounding agarwood extract, horsetail extract, clary sage extract, mulberry leaf extract and aluminum chlorohydrate.

Description

Technical Field

[0001] The invention belongs to the field of cosmetics, and in particular relates to an antibacterial and antiperspirant cream containing agarwood. Background Art

[0002] Although the antiperspirants currently on the market have a certain effect in controlling sweat secretion, there are still some shortcomings. Antiperspirants all contain metal salts or irritants, which may cause skin allergic reactions. At the same time, the working principle of antiperspirants is to inhibit the secretion of sweat glands by blocking and destroying pores, which may interfere with the normal function of sweat glands, causing blockage of sweat gland ducts and causing skin problems. In addition, the distribution and activity of sweat glands vary from person to person, so the effect of antiperspirants may vary from person to person. In addition, some antiperspirants may contain chemicals that are harmful to the human body, which may pose potential risks to health.

[0003] Therefore, there is an urgent need for an antibacterial and antiperspirant composition using plant extracts as main active ingredients to solve the above-mentioned shortcomings. Summary of the invention

[0004] The invention provides an antibacterial antiperspirant cream, which has the effects of antiperspirant, antibacterial and the like through natural ingredients extracted from plants.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] The invention provides an antibacterial and antiperspirant composition. The composition uses agarwood extract, horsetail extract, clary sage extract, mulberry leaf extract, aluminum chlorohydrate, peppermint oil and menthol to achieve the antibacterial and antiperspirant effects.

[0007] Preferably, the part from which the horsetail extract of the present invention is extracted is the aerial part.

[0008] Preferably, the clary sage extract of the present invention is extracted from flower buds and / or leaves.

[0009] Preferably, the mulberry leaf extract of the present invention is extracted from mulberry leaves.

[0010] Preferably, the mass ratio of the agarwood extract, horsetail extract, sage extract, and mulberry leaf extract of the present invention to aluminum chlorohydrate is 1:0.8-1.8:0.3-1:0.3-0.8:0.06-0.15.

[0011] Preferably, the present invention further comprises essence to adjust the fragrance of the antibacterial and antiperspirant composition of the present invention to make it more acceptable.

[0012] The invention also provides an antibacterial antiperspirant cream.

[0013] Preferably, the antibacterial antiperspirant composition contained in the antibacterial antiperspirant cream is ≤ 8%.

[0014] Preferably, the antibacterial antiperspirant cream further comprises a base, and the base comprises an oily base or an emulsion base or water.

[0015] Preferably, the semi-solid matrix comprises beeswax, honey wax and maltodextrin.

[0016] Preferably, the emulsion base comprises coconut oil, polysorbate 40 and sodium lauryl sulfate.

[0017] Preferably, the water-based matrix includes water, sodium lauryl sulfate and sodium dodecyl sulfate.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] The invention uses agarwood agarwood extract, horsetail extract, clary sage extract, mulberry leaf extract and aluminum chlorohydrate to compound, and the agarwood agarwood extract and the horsetail extract are compounded, and the terpenoid compounds and ketone compounds (mainly sesquiterpenoids and 2-(2-phenylethyl) chromone) in the agarwood agarwood are synergistically used with acid compounds such as succinic acid, fumaric acid, ferulic acid, caffeic acid, and vanillic acid in the horsetail to improve the antibacterial effect of the skin, control the oil secretion of the skin, and reduce the sweat secretion, and aluminum chlorohydrate is used as an adjuvant to further improve the antibacterial effect of the skin. One-step enhancement of antiperspirant effect; assisted by esters and alcohols contained in clary sage, further antibacterial and calming the skin; at the same time, ketones, polyphenols, polysaccharides and sterols contained in mulberry leaf extract keep the skin moisturized and healthy during the antiperspirant process; while antiperspirant and antibacterial, the fragrance produced by the combination of agarwood, clary sage and horsetail, such as clary sage alcohol, α-caryophyllene, β-eugenol, linalool, linalool acid and other volatile substances, helps to further soothe the mood and regulate emotions. DETAILED DESCRIPTION

[0020] In order to better understand the present invention, the present invention is further described below in conjunction with specific examples, wherein the terms used in the examples are for describing specific embodiments and do not constitute limitations on the scope of protection of the present invention.

[0021] The experimental methods in the following examples without specifying specific conditions are usually carried out under conventional conditions or under conditions recommended by the manufacturer. Unless otherwise specified, percentages and parts are calculated by weight.

[0022] Some raw materials and their sources are as follows:

[0023] Aquilaria sinensis extract: purchased from Xi'an Ruierli Bioengineering Co., Ltd.

[0024] Horsetail (Equisetum hyemale L.) extract: purchased from Shanyang Lianfeng Biotechnology Co., Ltd.

[0025] Salvia sclarea L. extract: purchased from Shanyang Lianfeng Biotechnology Co., Ltd.

[0026] Sage (Pseudognaphalium affine (D.Don) Anderb) extract: purchased from Shanyang Lianfeng Biotechnology Co., Ltd.

[0027] Mulberry leaf (Morus alba L.) extract: purchased from Shanyang Lianfeng Biotechnology Co., Ltd.

[0028] Ficus pandurata Hance leaf extract: purchased from Shanyang Lianfeng Biotechnology Co., Ltd.

[0029] Aluminum chlorohydrate: purchased from Wuxi Lanbo Chemicals Co., Ltd.

[0030] Menthol: purchased from Shenzhen Love Biotechnology Co., Ltd.

[0031] Peppermint oil: purchased from Shaanxi Tangyao Biotechnology Co., Ltd.

[0032] Staphylococcus epidermidis, Cutibacterium acnes, Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans (Robin) Berkhout were purchased from Beina Biotechnology Co., Ltd.

[0033] The invention provides an antibacterial and antiperspirant composition, and the preparation method thereof is as follows: weighing a certain amount of agarwood extract, horsetail extract, clary sage extract, mulberry leaf extract, aluminum chlorohydrate, menthol and peppermint oil.

[0034] Mix horsetail extract, sage extract and mulberry leaf extract evenly, add aluminum chlorohydrate and mix evenly; then drop agarwood extract, menthol and peppermint oil into the mixture and mix evenly.

[0035] The present invention also provides an antibacterial antiperspirant cream, and the specific preparation method thereof is as follows:

[0036] Add a certain mass percentage of the composition into the matrix, stir evenly, and sterilize at high temperature.

[0037] The present invention also provides a method for preparing a semi-solid matrix, an emulsion matrix, and an aqueous matrix, and the specific steps are as follows:

[0038] Semi-solid matrix preparation method: After beeswax, honey wax and maltodextrin are melted at a high temperature of ≥70°C, they are added in a mass fraction ratio of 4:2:6, stirred well and sterilized.

[0039] Preparation method of emulsion matrix: add polysorbate 40 and sodium lauryl sulfate into coconut oil, mix by ultrasonic vibration, and sterilize. The mass ratio of polysorbate 40 to sodium lauryl sulfate and coconut oil is 3:2:20.

[0040] Preparation method of water-based matrix: add sodium lauryl sulfate and sodium dodecyl sulfate into deionized water, mix by ultrasonic vibration, and sterilize. The mass ratio of sodium lauryl sulfate and sodium dodecyl sulfate to deionized water is 3:3:18.

[0041] The specific composition ratio by mass is shown in Table 1;

[0042] The specific mass percentage of the antibacterial antiperspirant cream is shown in Table 2.

[0043] Table 1

[0044]

[0045] Table 2

[0046]

[0047]

[0048] Safety Test

[0049] The 2015 "Technical Specification for Safety of Cosmetics" was used as a reference standard to evaluate the irritation of the cosmetics in Examples 4-14. The test method was a skin patch test, and 110 randomly distributed people aged 16-65 were tested.

[0050] Test method: Put the test substance into the patch tester, the dosage is 0.020-0.025g, cover the patch tester with the test substance on the back or forearm of the subject with non-irritating cloth-based tape, and gently press it with the palm to make it evenly adhere to the skin surface for 24 hours. After removing the test patch tester, observe the skin reaction after the indentation disappears 30 minutes later. If the result is negative, observe again 24 hours and 48 hours after the patch test.

[0051] Evaluation criteria:

[0052] Grade 0: negative reaction;

[0053] Grade 1: Suspicious reaction, only slight erythema;

[0054] Grade 2: weak positive reaction, erythema, infiltration, edema, and papules may be present;

[0055] Grade 3: Strong positive reaction, erythema, infiltration, edema, papules, and the reaction may extend beyond the test area;

[0056] Grade 4: Very strong positive reaction, obvious erythema, severe infiltration, edema, confluent herpes, and the reaction exceeds the test area.

[0057] Test results: Skin reactions were negative in all subjects.

[0058] The above test results show that Examples 4-14 provided by the present invention are mild and non-irritating to the skin and safe to use.

[0059] Cytotoxicity assay

[0060] Take the human immortalized epidermal cell line (HaCaT) with stable growth state and adjust the cell concentration to 1×10 5 / mL, inoculate 100μL per well into a 96-well cell culture plate (except the outermost well and blank control well). Add 200μL of PBS to the outermost well of the cell culture plate to reduce the evaporation of the culture medium. Place in a carbon dioxide incubator and culture at 37℃±1℃, CO2 concentration 5.0%±1% until the cell fusion reaches more than 90%. Add samples, the sample concentration is 1g / 100mL, set up blank control and sample group, 100μL per well. Start timing when adding samples (sample group) and PBS (blank control), keep the time interval between each two columns of sample additions consistent, and ensure that the intervention time of each test substance on the cells is 5min. After 5min, suck out the test substances in turn, add PBS to each well to wash the test substances, and wash twice. When using a vacuum suction pump or a discharge gun to suck out the liquid in the well, be careful to avoid sucking out the surviving cells and affecting the experimental results. Add 100μL of complete culture medium and 10μL of CCK-8 solution to each well, and place in an incubator to culture for about 2h. At a wavelength of 450 nm, the absorbance (OD) value was measured and the cell viability was calculated; the results are shown in Table 3.

[0061] Formula (1)

[0062] Table 3 Cytotoxicity test

[0063] Experimental Group HaCaT survival rate / % Example 4 98.27±3.12 Example 5 97.68±2.04 Example 6 96.37±2.24 Example 7 97.28±1.03 Example 8 97.39±4.12 Example 9 95.54±0.78 Example 10 96.78±1.22 Embodiment 11 97.24±3.27 Example 12 98.17±1.55 Example 13 96.71±1.27 Embodiment 14 97.24±1.13 blank 100

[0064] According to the experimental results, the cell survival rate is maintained above 95%. The antibacterial antiperspirant cream provided by the present invention has little effect on cell vitality.

[0065] Efficacy Experiment

[0066] 1. Antibacterial test

[0067] With reference to WS / T 650-2019, the inhibition ring test was used to wash the 24-hour fresh slant culture of Staphylococcus epidermidis, Propionibacterium acnes, Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans with PBS and dilute to 5.0×105 CFU / mL-5.0×10 6 CFU / mL is reserved.

[0068] Preparation of antibacterial sheets: Cut filter paper sheets with a diameter of 5 mm and a thickness not exceeding 4 mm, and immerse each group of 4 sheets in 10 mL of sample solution; the concentration of the sample solution is 1 g / 100 mL.

[0069] Preparation of negative control samples: Take samples of the same material, make discs (blocks) of the same size as the test group, and soak them in sterile water.

[0070] Use a sterile cotton swab to apply 5.0×10 5 CFU / mL-5.0×106CFU / mL bacterial suspension is evenly applied 3 times on the surface of a suitable culture medium plate. Each time it is applied, the plate should be rotated 60°, and finally the cotton swab should be applied around the edge of the plate. Cover the plate and dry it at room temperature for 5 minutes. For each test, place a contaminated plate, 4 test samples and 1 negative control sample on each plate, for a total of 5 samples. Use sterile tweezers to take the sample and paste it on the surface of the plate. The negative control sample is pasted in the center of the plate, and the test sample is pasted around. After pasting, use sterile tweezers to gently press the sample to make it close to the surface of the plate. The distance between the centers of each sample is more than 25mm, and the distance from the periphery of the plate is more than 15mm. Cover the plate, incubate at 36℃±1℃ for 16h~18h to observe the results, and repeat the test 3 times. Use a vernier caliper to measure the diameter of the antibacterial ring (including the patch) and record it. When measuring the antibacterial ring, a uniform and completely sterile antibacterial ring should be selected, and the diameter should be measured based on the outer edge of the antibacterial ring.

[0071] The negative control sample should not produce an antibacterial ring. If the diameter of the antibacterial ring of the test sample is greater than 7mm, it is judged to have an antibacterial effect; if the diameter of the antibacterial ring is ≤7mm, it is judged to have no antibacterial effect. If the antibacterial effect is found in the three repeated tests, it is judged to be qualified. See Table 4 for specific results.

[0072] Table 4 Antibacterial test

[0073]

[0074]

[0075] The results show that: the comparison between the embodiment and the comparative example shows that the embodiment is superior to the comparative example in terms of antibacterial performance. Therefore, the effect achieved by the present invention cannot be achieved by any default in the preparation according to the ratio specified in the present invention. And the default of the comparative example also proves that there is a certain synergistic effect between the ratios specified in the present invention.

[0076] 2. Antiperspirant efficacy evaluation experiment

[0077] 170 volunteers were selected (half male and half female, 5 male volunteers and five female volunteers were randomly selected to try the product). According to the random assignment, half of the subjects received antibacterial antiperspirant cream treatment on the left armpit (experimental group) and placebo treatment on the right side (control group), and half of the subjects received antibacterial antiperspirant cream treatment on the right armpit (experimental group) and placebo treatment on the left side (control group). The test products and placebos were distributed to the subjects, and the subjects were informed of the precautions for the test products or placebos: once in the morning and evening daily for 4 days. Before each application, the armpit needs to be properly cleaned and dried with a tissue. The product or placebo should be applied within 8×20mm2 of the center of the armpit.

[0078] The subjects wore uniform clothing and placed fiber pads under their armpits to absorb sweat. The subjects were placed in a hot room with a temperature of 38.0±1℃ and a relative humidity of 30-40% for 60 minutes, followed by a 20-minute adaptation phase. The fiber pads used in the adaptation phase were disposable and no further evaluation was performed.

[0079] After the adaptation phase, place an absorbent pad with an area of ​​6×12 cm2 in a dry bottle, weigh and record, and then accurately place the pad under the subject's two armpits. Arrange the subject to continue the test in the experimental environment for 20 minutes, and the sweat is collected by the pad. After the collection phase, place the pad in a dry bottle, weigh it again on a precision balance, and record.

[0080] How to calculate sweat output:

[0081] Sweat volume = pad weight after the collection phase - pad weight before the collection phase.

[0082] Calculation method of sweat reduction rate:

[0083] Sweating reduction rate = (axillary sweating of the control group - axillary sweating of the experimental group) / axillary sweating of the control group.

[0084] The results are shown in Table 5.

[0085] Table 5

[0086]

[0087]

[0088] The results show that: the comparison between the embodiment and the comparative example shows that the embodiment is better than the comparative example in terms of antiperspirant performance, so the effect achieved by the present invention cannot be achieved by preparing with the ratio defined in the present invention, and any default is also proved by the default of the comparative example that there is a certain synergistic effect between the ratios defined in the present invention.

[0089] The embodiments described above are part of the embodiments of the present application, rather than all of the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the present application for protection, but merely represents the selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the present application.

Claims

1. An antibacterial and antiperspirant composition, characterized in that: The composition comprises the following components in parts by weight: Agarwood extract: 10-15 parts; Equisetum arvense extract: 12-18 parts; Clary sage extract: 5-10 parts; Mulberry leaf extract: 5-8 parts; Aluminum chlorohydrate: 1-1.5 parts.

2. The composition according to claim 1, characterized in that The extraction site of the horsetail extract is the aerial part; the extraction site of the clary sage extract is the leaves and / or flower buds; and the extraction site of the mulberry leaf extract is the leaves.

3. The composition according to claim 1, characterized in that The composition further comprises 5-10 parts of cooling agent.

4. The composition according to claim 3, characterized in that The cooling agent is menthol and / or peppermint oil.

5. An antibacterial antiperspirant cream comprising the composition according to any one of claims 1 to 4.

6. The antibacterial antiperspirant cream according to claim 5, characterized in that: The composition accounts for ≤8% by mass in the paste.

7. The antibacterial antiperspirant cream according to claim 5, characterized in that: Also included are carrier matrices.

8. The antibacterial antiperspirant cream according to claim 7, characterized in that: The carrier matrix includes a semi-solid matrix, an emulsion matrix, or an aqueous matrix.

9. The antibacterial antiperspirant cream according to claim 8, characterized in that: When the carrier matrix is ​​an oil-type matrix, it includes the following raw material components: beeswax, maltodextrin, and beeswax; when the carrier matrix is ​​an emulsion-type matrix, it includes the following raw material components: glycerin, polysorbate 40, and sodium lauryl sulfate; when the carrier matrix is ​​a water-type matrix, it includes the following raw material components: water, sodium lauryl sulfate, and sodium dodecyl sulfate.

10. The antibacterial antiperspirant cream according to claim 5, characterized in that: The paste is a semi-solid paste or a liquid paste.

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