Sample detection system and method

Abstract

This application discloses a sample detection system and method, applied in the field of spectral analysis technology. The system includes a light projection module, a sample carrier, a signal acquisition module, and a sample detection module. The light projection module emits a laser signal of the target wavelength and focuses it onto the sample to be tested on the sample carrier. The signal acquisition module uses a front-facing light-receiving probe and at least one side-facing light-receiving probe to acquire Raman scattering and Rayleigh scattering signals generated by the sample to be tested. The sample detection module performs spectral composition analysis on the acquired scattered light signals from the sample to obtain the Raman scattering spectrum, and detects the sample based on the Raman scattering spectrum. This application overcomes the drawback of weak signal intensity in related technologies, effectively improving Raman signal intensity, especially for rough samples, enabling efficient and accurate detection.

Description

Technical Field

[0001] This application relates to the field of spectral analysis technology, and in particular to a sample detection system and method. Background Technology

[0002] With the rapid development of spectroscopic analysis technology, portable Raman spectrometers, which have advantages such as small size, high speed, and on-site operation, can achieve pure qualitative analysis, highly quantitative analysis, and determination of molecular structure of samples such as pharmaceuticals, food, and hazardous materials using Raman scattering spectroscopy. They are widely used in various technical fields such as chemistry, physics, biology, and medicine.

[0003] To improve sample detection accuracy, related technologies typically adjust focusing precision, such as by attaching a fixed-length sleeve to the probe to fix the measurement distance and ensure focusing accuracy. However, when using this type of probe, the sleeve must be in contact with the sample. This makes precise focusing impossible for rough samples with uneven surfaces, requiring pretreatment before detection. Even with pretreatment, the weak intensity of the collected scattered light signal prevents high-precision detection of rough samples. Therefore, these technologies are not only cumbersome to operate but also lack high detection accuracy.

[0004] Therefore, efficiently and accurately detecting rough samples is a technical problem that needs to be solved by professionals in this field.

[0005] It should be noted that the information disclosed in the background section above is only used to enhance the understanding of the background of this application, and therefore may include information that does not constitute prior art known to those skilled in the art. Summary of the Invention

[0006] This application provides a sample testing system and method that enables efficient and accurate testing of rough samples.

[0007] To solve the above-mentioned technical problems, this application provides the following technical solution:

[0008] This application provides a sample detection system, including a light projection module, a sample carrying device, a signal acquisition module, and a sample detection module;

[0009] The light projection module is used to emit a laser signal of the target wavelength and focus the laser signal onto the sample to be tested on the sample carrier device;

[0010] The signal acquisition module includes a front light-receiving probe and at least one side light-receiving probe for acquiring the scattered light signal generated by the sample under test. The scattered light signal includes Raman scattering and Rayleigh scattering signals. The front light-receiving probe is deployed in the forward optical path of the scattered light signal from the sample under test, and the axis of the front light-receiving probe is perpendicular to the surface of the sample support device. The side light-receiving probes are deployed in the respective lateral optical paths of the scattered light signal from the sample under test.

[0011] The sample detection module is used to perform spectral composition analysis on the scattered light signal of the sample to be tested, obtain the Raman scattering spectrum, and detect the sample to be tested based on the Raman scattering spectrum.

[0012] For example, the light projection module includes a laser, a beam splitter, and a focusing element;

[0013] The laser emits a laser signal at the target wavelength;

[0014] The beam splitter reflects the laser signal of the target wavelength to the focusing element to obtain the reflected light signal;

[0015] The focusing element focuses the reflected light signal onto the sample to be tested and collimates the forward scattered light signal of the sample to be tested. The collimated light signal enters the front light receiving probe through the beam splitting element.

[0016] The front-facing light-receiving probe is axially collimated with the focusing element, and the beam center of the scattered light refracted by the beam-splitting element is coaxial.

[0017] For example, the light projection module includes a monochromatic laser, a dichroic mirror, and a laser focusing lens;

[0018] The monochromatic laser emits a monochromatic laser signal of the target wavelength;

[0019] The dichroic mirror is matched with the target wavelength, reflecting light signals less than or equal to the target wavelength to the laser focusing lens, and transmitting light signals greater than the target wavelength.

[0020] The laser focusing lens focuses the reflected light signal into a focused spot and collimates the scattered light emitted from the front of the focused spot;

[0021] The front-facing light-receiving probe is axially collimated with the laser focusing lens, and the beam center of the scattered light refracted by the dichroic mirror is coaxial; the focused light spot is located on the surface of the sample to be tested.

[0022] For example, the front-facing light-receiving probe includes, in sequence, a first long-pass filter, a first focusing lens, and a first optical fiber according to the direction of propagation of the scattered light signal;

[0023] The scattered light emitted from the front of the focused spot of the focusing element is collimated, passes through the beam splitter, is projected onto the first long-pass filter, and is then focused by the first focusing lens onto the entrance of the first optical fiber.

[0024] For example, the signal acquisition module includes a first side light receiving probe, a second side light receiving probe, and a third side light receiving probe; the area covered by the scattered light emitted from the side of the focused light spot on the surface of the sample to be tested is divided into a first region, a second region, and a third region;

[0025] The first side-mounted light-collecting probe is deployed at a first target position within a first area covered by the scattered light emitted from the side of the focused light spot on the surface of the sample to be tested, in order to collect the scattered light within the first area;

[0026] The second side-mounted light-collecting probe is deployed at a second target position in a second region covered by the scattered light emitted from the side of the focused light spot on the surface of the sample to be tested, in order to collect the scattered light within the second region;

[0027] The third side-mounted light-collecting probe is deployed at the third target position in the third region covered by the scattered light emitted from the side of the focused light spot on the surface of the sample to be tested, in order to collect the scattered light within the third region.

[0028] For example, the mounting base of the side light-receiving probe is a multi-functional movable base;

[0029] The side light-receiving probe adjusts its spatial position during the horizontal and vertical movement of the multifunctional movable base, and adjusts its acquisition angle during the rotation of the multifunctional movable base.

[0030] For example, the sample detection module includes an optical signal transmission element, a spectrometer, and a computing device;

[0031] The signal acquisition module is connected to the spectrometer through the optical signal transmission element. The spectrometer performs spectral composition analysis on the scattered light signal of the sample to be tested and sends the spectral analysis data to the computing device.

[0032] The computing device generates Raman scattering spectra based on the spectral analysis data.

[0033] For example, the sample carrying device includes a retractable movable base;

[0034] During the horizontal and / or vertical movement of the retractable movable base, the sample carrier device adjusts its spatial position.

[0035] For example, the side-mounted light receiving probe includes, in sequence according to the direction of the propagation of the scattered light signal, a collimating lens, a second long-pass filter, a second focusing lens, and a second optical fiber;

[0036] The focal point of the collimating lens is located at the focused spot on the surface of the sample to be tested. The collimating lens collimates the scattered light emitted from the side of the focused spot and projects it onto the second long-pass filter, which is then focused by the second focusing lens onto the entrance of the second optical fiber.

[0037] The collimating lens has the same numerical aperture as the focusing element of the light projection module.

[0038] This application also provides a sample testing method, applied to the sample testing system described in any of the preceding claims, comprising:

[0039] When the sample to be tested is detected to be placed in the sample placement area of ​​the sample carrier, a laser signal of the target wavelength is emitted and the laser signal is focused onto the sample to be tested.

[0040] Raman scattering spectra are generated based on the spectral composition analysis data of the Raman scattering and Rayleigh scattering signals generated by the sample under test.

[0041] The advantage of the technical solution provided in this application is that multiple light-receiving probes are deployed on the front and side optical paths of the scattered light signal of the sample to be tested, making full use of the space where the scattered light can be measured. Especially for rough samples, the Raman scattered light from the side contains a lot of material information, so scattered light containing richer material information of the sample to be tested can be collected. The Raman scattering spectrum obtained by analyzing the spectral composition of these scattered lights can more accurately reflect the sample to be tested and improve the accuracy of the sample.

[0042] Furthermore, this application also provides a corresponding implementation method for the sample detection system, which further makes the system more feasible, and the method has corresponding advantages.

[0043] The technical features mentioned above, those to be mentioned below, and those shown individually in the accompanying drawings can be arbitrarily combined, provided that the combined technical features are not contradictory. All feasible combinations of features are the technical content explicitly described herein. Any one of the multiple sub-features contained in the same statement can be applied independently, without necessarily being applied together with other sub-features. It should be understood that the above general description and the following detailed description are merely exemplary and do not limit this application. Attached Figure Description

[0044] To more clearly illustrate the technical solutions of this application or related technologies, the drawings used in the description of the embodiments or related technologies will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0045] Figure 1 A schematic diagram of the structural framework of one embodiment of the sample testing system provided in this application;

[0046] Figure 2 A schematic diagram of one embodiment of the sample testing system provided in this application;

[0047] Figure 3 A schematic diagram of one embodiment of the front-facing light-receiving probe provided in this application;

[0048] Figure 4 A schematic diagram of one embodiment of the side-mounted light-receiving probe provided in this application;

[0049] Figure 5 A schematic flowchart of a sample testing method provided in this application;

[0050] Figure 6 A schematic diagram of the Raman scattering spectrum generated by the scattered light of diamond powder collected by the side light-receiving probe, provided for this application;

[0051] Figure 7 A schematic diagram of the Raman scattering spectrum generated by the scattered light of diamond powder collected by the front-facing light-collecting probe, provided for this application;

[0052] Figure 8 A schematic diagram of the Raman scattering spectrum generated from the scattered light of diamond powder collected by the front light-collecting probe and three side light-collecting probes, provided for this application;

[0053] Figure 9A schematic diagram of the Raman scattering spectrum generated by the scattered light from a silicon carbide crystal (smooth, sheet-like surface) collected by a side-mounted light-collecting probe, provided in this application.

[0054] Figure 10 A schematic diagram of the Raman scattering spectrum generated by the scattered light from a silicon carbide crystal (smooth, sheet-like surface) collected by a front-facing light-collecting probe, provided in this application.

[0055] Figure 11 A schematic diagram of the Raman scattering spectrum generated by the scattered light from a silicon carbide crystal (smooth, sheet-like surface) collected by a front-facing light-collecting probe and three side-facing light-collecting probes, provided for this application.

[0056] Among them, 101-light projection module, 102-signal acquisition module, 103-sample detection module, 104-sample carrying device, 1-laser, 2-dichroic mirror, 3-laser focusing lens, 4-sample stage, 5-front light receiving probe, 6-side light receiving probe, 7-optical signal transmission element, 8-spectrometer, 9-computing device, 5-1-first long-pass filter, 5-2-first focusing lens, 5-3-first optical fiber, 6-1-collimating lens, 6-2-second long-pass filter, 6-3-second focusing lens, 6-4-second optical fiber. Detailed Implementation

[0057] To enable those skilled in the art to better understand the present application, the present application will be further described in detail below with reference to the accompanying drawings and specific embodiments. The terms "first," "second," "third," etc., used in the specification and the aforementioned drawings are used to distinguish different objects, not to describe a specific order. Furthermore, the terms "comprising" and "having," and any variations thereof, are intended to cover non-exclusive inclusion. The term "exemplary" means "serving as an example, embodiment, or illustration." Any embodiment described herein as "exemplary" is not necessarily to be construed as superior to or better than other embodiments.

[0058] Raman spectroscopy is a type of scattering spectroscopy. Based on the Raman scattering effect, Raman spectroscopy analysis can determine the types of molecules that make up a substance being analyzed. A Raman spectrometer is the instrument used for Raman spectroscopy analysis. When detecting a sample, a Raman spectrometer first efficiently transmits and focuses a laser beam onto the sample, then collects the Raman scattered light signal and transmits it to the spectrometer for spectral analysis, obtaining the relevant detection results for the substance being analyzed.

[0059] However, generally speaking, Raman scattering light signals are very weak, especially for miniaturized and portable Raman detection devices. The Raman scattering light signals they can collect are weak and cannot meet the high-precision sample detection needs of users, especially for rough samples, resulting in a poor user experience.

[0060] Therefore, this application arranges multiple light-collecting probes on the front and side optical paths of the scattered light signal of the sample under test, making full use of the measurable space, so that more scattered light is collected, resulting in an optical signal containing more of the sample under test, thereby improving the intensity and detection capability of the scattered light signal. After introducing the technical solution of this application, various non-limiting embodiments of this application are described in detail below. To better illustrate this application, numerous specific details are given in the following detailed embodiments. Those skilled in the art should understand that this application can be implemented without these specific details. In other examples, methods, means, components, and circuits well known to those skilled in the art are not described in detail in order to highlight the main points of this application.

[0061] Please see first. Figure 1 , Figure 1 The present application provides a flowchart of a sample testing method, which may include the following:

[0062] The sample detection system may include a light projection module 101, a signal acquisition module 102, a sample detection module 103, and a sample carrying device 104.

[0063] The sample carrier 104 is used to carry and fix the sample to be tested, and the light projection module 101 is used to emit a laser signal of the target wavelength and focus the laser signal onto the sample to be tested on the sample carrier 104. The target wavelength can be selected according to the sample to be tested, for example, 532±△nm, 635±△nm, 785±△nm, 1064±△nm, where △ represents a small fluctuation value. The laser signal can be ultraviolet light, infrared light, or visible light, none of which will affect the realization of this application.

[0064] The signal acquisition module 102 of this embodiment includes a front light-receiving probe and at least one side light-receiving probe. The signal acquisition module 102 can be used to acquire the scattered light signal generated by the sample under test. The number, spatial position, and acquisition angle of the front light-receiving probe and the side light-receiving probe in this embodiment can be flexibly adjusted according to actual needs. The front light-receiving probe is deployed in the forward optical path of the scattered light signal of the sample under test, and its axis is perpendicular to the surface of the sample carrying device 104; each side light-receiving probe is deployed in each lateral optical path of the scattered light signal of the sample under test. Considering system miniaturization and signal collection effectiveness, for example, there can be only one front light-receiving probe, and the number of side light-receiving probes can be increased or decreased according to needs, but not less than one. The front light-receiving probe and at least one side light-receiving probe acquire the scattered light signal of the sample under test, which includes Raman scattered light signal and Rayleigh scattered light signal, and transmit the Raman scattered light signal and Rayleigh scattered light signal to the sample detection module 103.

[0065] In this embodiment, the sample detection module 103 is used to perform spectral composition analysis on the scattered light signal of the sample to be tested, obtain the Raman scattering spectrum, and detect the sample based on the Raman scattering spectrum. The detection of the sample to be tested includes, but is not limited to, purely qualitative analysis, highly quantitative analysis, and determination of molecular structure. Raman scattering spectroscopy relies on the inelastic scattering of the received scattered light signal by the molecules of the sample to be tested. The molecules in the sample to be tested undergo energy transfer with photons, and their vibrational dynamics change in different ways and to different degrees, and then scatter light of different frequencies. Among them, the vibrational dynamics can be the wobbling and twisting of atoms, the wobbling and vibration of chemical bonds. The frequency change depends on the characteristics of the sample to be tested. Different types of atomic groups vibrate in unique ways, thus producing scattered light with a specific difference from the incident light frequency. Based on Raman scattering spectroscopy, the types of molecules that make up the sample to be tested can be identified, realizing purely qualitative analysis, highly quantitative analysis, and determination of molecular structure of the sample to be tested.

[0066] In the technical solution provided in this application, multiple light-collecting probes are deployed on the front and side optical paths of the scattered light signal of the sample to be tested, making full use of the space where the scattered light can be measured. Especially for rough samples, the Raman scattered light from the side contains a lot of material information, so scattered light containing richer material information of the sample to be tested can be collected. The Raman scattering spectrum obtained by analyzing the spectral composition of these scattered lights can more accurately reflect the sample to be tested and improve the accuracy of the sample.

[0067] The above embodiments do not limit the structure of the light projection module 101. This embodiment also provides an exemplary structural composition of the light projection module 101, which may include the following:

[0068] The light projection module 101 may include a laser, a beam splitter, and a focusing element; the laser emits a laser signal of the target wavelength; the beam splitter reflects the laser signal of the target wavelength to the focusing element; the focusing element focuses the reflected light signal onto the sample to be tested and collimates the forward scattered light signal of the sample to be tested that returns from the optical path; the collimated light signal enters the front light receiving probe through the beam splitter.

[0069] In this embodiment, the laser emits a laser signal of any desired wavelength, which is the target wavelength. The beam splitter changes the incident direction of the laser signal emitted by the laser and filters the laser signal, reflecting only the laser signal of the target wavelength to the focusing element. Light signals of other wavelengths are not transmitted and do not enter the sample under test. The focusing element focuses the incident laser signal onto the sample under test. The sample under test is irradiated by the laser, generating scattered light signals, including Raman scattered light signals and Rayleigh scattered light signals. The Raman and Rayleigh scattered light signals are again incident on the focusing element in the forward optical path. The focusing element collimates the Raman and Rayleigh scattered light signals, which then enter the beam splitter. Finally, the beam splitter enters the front-facing light-receiving probe, whose axis is collimated with the focusing element, and the beam centers of the scattered light refracted by the beam splitter are coaxial.

[0070] For example, the laser in the above embodiments emits monochromatic laser light, meaning the laser can be a monochromatic laser with a wavelength selected from the ultraviolet, visible, or infrared spectrum. Preferred laser wavelengths are 532nm, 635nm, 785nm, and 1064nm. The beam splitting element can be a dichroic mirror, which separates light according to a specific wavelength, dividing the light source into transmitted or reflected light. It transmits almost completely light of the designed wavelength while reflecting almost completely light of other wavelengths, or vice versa. That is, the dichroic mirror is selected according to the target laser wavelength, ensuring it reflects most of the laser signal while allowing most electromagnetic waves longer than the laser wavelength to pass through. The focusing element can be a laser focusing lens, which converges the laser light reflected by the dichroic mirror into a focused spot located on the surface of the sample to be tested. The laser focusing lens collimates some of the scattered light emitted from the front of the focused spot. In other words, an exemplary structure of the light projection module 101 may include a monochromatic laser, a dichroic mirror, and a laser focusing lens; the monochromatic laser emits a monochromatic laser signal of the target wavelength; the dichroic mirror matches the target wavelength, reflecting light signals less than or equal to the target wavelength to the laser focusing lens, and transmitting light signals greater than the target wavelength; the laser focusing lens focuses the reflected light signal into a focused spot and collimates the scattered light emitted from the front on the focused spot; wherein, the axis of the front light receiving probe is collimated with the laser focusing lens, and the beam center of the scattered light refracted by the dichroic mirror is coaxial; the focused spot is located on the surface of the sample to be tested.

[0071] As can be seen from the above, this embodiment uses a laser focusing lens and a dichroic mirror to focus most of the laser signal emitted by the laser onto the sample to be tested, which is beneficial to improving the detection accuracy of the sample.

[0072] The above embodiments do not limit the structure of the signal acquisition module 102. This embodiment also provides an exemplary structural composition of the signal acquisition module 102, which may include the following:

[0073] In this embodiment, the front light receiving probe of the signal acquisition module 102 includes a first long-pass filter, a first focusing lens, and a first optical fiber in sequence according to the direction of propagation of the scattered light signal; wherein, the scattered light emitted from the front on the focused spot of the focusing element is collimated, passes through the beam splitter, is projected onto the first long-pass filter, and is then focused by the first focusing lens onto the entrance of the first optical fiber.

[0074] The first long-pass filter can be selected according to the target wavelength to reflect most of the laser signal of the target wavelength, while allowing most of the electromagnetic waves longer than the target wavelength to pass through. The first focusing lens focuses the scattered light collimated by the laser focusing lens, i.e., the focusing element, at the first optical fiber entrance. Of course, if the optical signal transmission element inside the front-facing light receiving probe is not an optical fiber, the first focusing lens will focus the received laser signal onto its internal optical signal transmission element.

[0075] To maximize the collection of scattered light signals, fully utilize the spatial distribution characteristics of scattered light, and meet the requirements for system miniaturization and portability, three side-mounted light-receiving probes can be configured. Therefore, the signal acquisition module 102 includes a first side-mounted light-receiving probe, a second side-mounted light-receiving probe, and a third side-mounted light-receiving probe. The area covered by the scattered light emitted from the side of the focused light spot on the surface of the sample under test is divided into a first region, a second region, and a third region. The first side-mounted light-receiving probe is deployed at a first target position within the first region covered by the scattered light emitted from the side of the focused light spot on the surface of the sample under test, to collect the scattered light within the first region. The second side-mounted light-receiving probe is deployed at a second target position within the second region covered by the scattered light emitted from the side of the focused light spot on the surface of the sample under test, to collect the scattered light within the second region. The third side-mounted light-receiving probe is deployed at a third target position within the third region covered by the scattered light emitted from the side of the focused light spot on the surface of the sample under test, to collect the scattered light within the third region. The first, second, and third target positions are determined based on the acquisition range of the light-receiving probes and the position and size of the corresponding regions. Of course, the number of side light-receiving probes can be increased or decreased as needed, without affecting the implementation of this application.

[0076] For example, each side-mounted light receiving probe may contain, in sequence according to the direction of scattered light signal propagation, a collimating lens, a second long-pass filter, a second focusing lens, and a second optical fiber. The focal point of the collimating lens is located at the focused spot on the surface of the sample to be tested. After the collimating lens collimates the scattered light emitted from the side of the focused spot, it is projected onto the second long-pass filter and then focused by the second focusing lens onto the entrance of the second optical fiber. The collimating lens has the same numerical aperture NA as the focusing element of the light projection module.

[0077] In this embodiment, the collimating lens inside the side-mounted light receiver is focused at the laser focusing spot, collimating the collected scattered light. The second long-pass filter can be selected according to the laser wavelength to reflect most of the laser light while allowing most electromagnetic waves longer than the laser wavelength to pass through. The second focusing lens focuses the scattered light, collimated by the collimating lens, onto the fiber optic inlet. Of course, if the optical signal transmission element inside the side-mounted light receiver is not an optical fiber, the second focusing lens will focus the received laser signal onto its internal optical signal transmission element.

[0078] For example, to further improve the light collection efficiency of the side-mounted light receiver, the mounting base of the side-mounted light receiver is a multi-functional movable base. This multi-functional movable base can be equipped with pulleys, guide rails, and casters, allowing the base to move horizontally. It can also be equipped with a height-adjustable telescopic rod, allowing the side-mounted light receiver to move vertically (Z-axis) by adjusting the rod's height. Furthermore, the multi-functional movable base can be equipped with a rotating component, which can rotate the side-mounted light receiver 360°. Alternatively, the connection between the side-mounted light receiver and the base can be made flexible, allowing the selection of the side-mounted light receiver's acquisition angle by twisting and rotating the flexible connection. By using a multi-functional movable base, the side-mounted light receiver adjusts its spatial position during horizontal and vertical movement of the multi-functional movable base, and adjusts its acquisition angle during the rotation of the multi-functional movable base.

[0079] As can be seen from the above, by limiting the structure of the front and side light-receiving probes in the signal acquisition module, this embodiment makes the whole system more flexible, more practical, and provides a better user experience. In addition, while ensuring the maximum collection of scattered light signals, it can also be developed towards miniaturization and portability.

[0080] The above embodiments do not limit the structure of the sample detection module 103. This embodiment also provides an exemplary structural composition of the sample detection module 103, which may include the following:

[0081] The sample detection module 103 may include an optical signal transmission element, a spectrometer, and a computing device; the signal acquisition module is connected to the spectrometer through the optical signal transmission element, the spectrometer performs spectral composition analysis on the scattered light signal of the sample to be tested, and sends the spectral analysis data to the computing device; the computing device generates a Raman scattering spectrum based on the spectral analysis data.

[0082] In this embodiment, the optical signal transmission element can be, for example, an optical fiber or a lens, and the computing device can be any device with computing capabilities, such as a personal computer, server, or smart device, including processors, microprocessors, and digital signal processors. This does not affect the implementation of this application. The signal acquisition module 102 transmits all collected scattered light to a spectrometer via an optical fiber or lens. The spectrometer analyzes and measures the spectral information of the scattered light, and the computing device then calculates the Raman scattering spectrum.

[0083] Furthermore, to improve the practicality of the sample testing system, the base of the sample carrier 104 is a telescopic movable base. The telescopic movable base may be equipped with pulleys, guide rails, or casters, so that the sample carrier 104 can move in the horizontal direction. It may also be equipped with a telescopic rod that can be adjusted in height, so that the sample carrier 104 can move in the vertical direction, i.e., the Z direction, by adjusting the height of the rod. This allows the sample carrier to adjust its spatial position during the horizontal and / or vertical movement of the telescopic movable base.

[0084] As can be seen from the above, the sample carrier device for placing the sample to be tested in this embodiment has the ability to move in the XY direction and adjust in the vertical Z direction, which makes it more practical.

[0085] To enable those skilled in the art to more clearly understand the implementation of this application, an exemplary implementation method is also provided. Please refer to [link / reference]. Figure 2 In this embodiment, the sample carrying device is a sample stage, the computing device is a computer, there are three side light-receiving probes and one front light-receiving probe, and the optical signal transmission element is an optical fiber. The sample detection system of this embodiment may include the following:

[0086] like Figure 2As shown, the sample detection system may include a laser 1, a dichroic mirror 2, a laser focusing lens 3, a sample stage 4, a front light-receiving probe 5, side light-receiving probes 6, an optical signal transmission element 7, a spectrometer 8, and a computing device 9. The laser emitted by the laser 1 has its incident direction changed by the dichroic mirror 2 and focused onto the sample to be tested on the sample stage 4 by the laser focusing lens 3. The sample to be tested is irradiated by the laser, resulting in Raman scattering and Rayleigh scattering. The scattered light is received by one front light-receiving probe 5 and three side light-receiving probes 6. All collected scattered light is transmitted to the spectrometer 8 by the optical signal transmission element 7. The spectrometer 8 analyzes the spectral composition information, and the computing device 9 processes the data to obtain the Raman scattering spectrum. The front light-receiving probe 5 is axially perpendicular to the surface of the sample stage 4 and is collimated with the laser focusing lens 3, with the beam center of the scattered light refracted by the dichroic mirror 2 being coaxial.

[0087] like Figure 3 As shown, the front-facing light-receiving probe 5 contains a first long-pass filter 5-1, a first focusing lens 5-2, and a first optical fiber 5-3 arranged sequentially. The laser focusing lens 3 collimates part of the scattered light emitted from the front on the focusing spot, transmits it through the dichroic mirror 2, passes through the first long-pass filter 5-1, and is then focused by the first focusing lens 5-2 onto the entrance of the first optical fiber 5-3.

[0088] like Figure 4 As shown, the side-mounted light receiver 6 contains, in sequence, a collimating lens 6-1, a second long-pass filter 6-2, a second focusing lens 6-3, and a second optical fiber 6-4. The collimating lens 6-1 is focused at the laser focusing spot, collimating the collected scattered light. The light then passes through the second long-pass filter 6-2 and is focused by the second focusing lens 6-3 onto the entrance of the second optical fiber 6-4. The collimating lens 6-1 of the side-mounted light receiver 6 has the same NA value as the laser focusing lens 3.

[0089] As can be seen from the above, the sample detection system of this embodiment can collect more Raman scattered light from the sample to be tested, thereby increasing the Raman signal intensity. The entire sample detection system is small in size and highly practical.

[0090] Furthermore, based on the above-described sample detection system, this application also provides a sample detection method applicable to the sample detection system described in any of the above embodiments, as detailed in [link to details]. Figure 5 . Figure 5 The present application provides a flowchart of a sample testing method, which may include the following:

[0091] S501: When the sample to be tested is detected to be placed in the sample placement area, a laser signal of the target wavelength is emitted and focused onto the sample to be tested.

[0092] In this embodiment, a robotic arm or a telescopic moving stage can be provided. When the user clicks the placement button, the telescopic moving stage can be deployed. The telescopic moving stage can be a sample stage or connected to a sample stage. When the image acquisition device or other target detection method detects that the telescopic moving stage is used to place the sample to be tested, the telescopic moving stage is controlled to move the sample to be tested to a pre-defined sample placement area. Similarly, when the user is detected placing the sample to be tested in the placement area, the robotic arm is controlled to place the sample to be tested in the pre-defined sample placement area. When the image acquisition device detects the presence of the sample to be tested in the sample placement area, the laser can be controlled to emit a laser signal. The emitted laser signal is focused onto the sample to be tested by internal components such as a dichroic mirror and a focusing lens.

[0093] S502: Generate a Raman scattering spectrum based on the spectral composition analysis data of the Raman scattering and Rayleigh scattering signals generated by the sample to be tested.

[0094] When the sample is irradiated by a laser, it will generate a scattered light signal, which is sent to the Raman analyzer by the front light receiving probe inside the system. The Raman analyzer performs spectral analysis on the scattered light signal and sends the spectral analysis data to the processor inside the system. The processor generates a Raman scattering spectrum based on the spectral composition analysis data of the Raman scattered light signal and the Rayleigh scattered light signal generated by the sample.

[0095] In this embodiment, the above-described sample detection method can be encapsulated as one or more program modules. These program modules are stored in a storage medium and executed by one or more processors to complete the sample detection method disclosed in the embodiment. The program module referred to in this application is a series of computer program instruction segments capable of performing a specific function.

[0096] As can be seen from the above, this embodiment can achieve efficient and accurate detection of samples.

[0097] It should be noted that there is no strict order of execution for the steps in this application. As long as they conform to a logical order, these steps can be executed simultaneously or in a certain preset order. Figure 5 This is just an illustrative example and does not mean that this is the only possible execution order.

[0098] To verify the validity of this application, the present invention is based on Figure 2 The illustrated sample detection system, when used to detect different samples, may include the following:

[0099] Raman spectra of diamond powder were tested using a sample detection system.

[0100] Laser 1 emits a monochromatic laser with a wavelength of 532 nm and a power of 13 mW. The incident direction is changed by a dichroic mirror 2, which has high reflectivity for light below 538 nm and high transmittance for light above 538 nm. The laser is then focused onto the surface of diamond powder by a laser focusing lens 3. The diamond powder is placed on a sample stage 4. The diamond sample is irradiated by the laser, resulting in Raman and Rayleigh scattering. The scattered light is received by a front-facing light-collecting probe 5 and a side-facing light-collecting probe 6. All collected scattered light is transmitted to a spectrometer 8 via an optical signal transmission element 7 for spectral composition analysis. The integration time of the spectrometer 8 is 3 seconds, and the measurement wavenumber range is set to 400 cm⁻¹. -1 -2000cm -1 Finally, the Raman scattering spectrum is obtained by processing the data using computing device 9. Figure 6 To shield the front light-receiving probe 5, the diamond powder Raman spectrum and signal intensity were measured using the side light-receiving probe 6. Figure 7 To shield the side light-receiving probe 6, the diamond powder Raman spectrum and signal intensity were measured using the front light-receiving probe 5. Figure 8 The Raman spectrum and signal intensity of diamond powder were measured together by the front-facing light-receiving probe 5 and the side-facing light-receiving probe 6. Based on Figures 6-8 It can be seen that with the addition of the side light-receiving probe 6, the diamond powder signal is enhanced by approximately 3.58 times compared to having only the front light-receiving probe 5.

[0101] The Raman spectrum of silicon carbide crystals (smooth, plate-like surface) was tested using a sample detection system.

[0102] Laser 1 emits a monochromatic laser with a wavelength of 532 nm and a power of 13 mW. The incident direction is changed by a dichroic mirror 2, which has high reflectivity for light below 538 nm and high transmittance for light above 538 nm. The laser is then focused onto the surface of a smooth, plate-like silicon carbide crystal by a laser focusing lens 3. The silicon carbide crystal is placed on a sample stage 4. The laser irradiation of the crystal produces Raman and Rayleigh scattering. The scattered light is received by a front-facing light-collecting probe 5 and a side-facing light-collecting probe 6. All collected scattered light is transmitted to a spectrometer 8 via an optical signal transmission element 7 for spectral composition analysis. The integration time of the spectrometer 8 is 3 seconds, and the measurement wavenumber range is set to 400 cm⁻¹. -1 -2000cm -1 Finally, the Raman scattering spectrum is obtained by processing the data using computing device 9. Figure 9 To shield the front light-receiving probe 5, the Raman spectrum and signal intensity of the silicon carbide crystal (smooth, sheet-like surface) were measured using the side light-receiving probe 6. Figure 10 To shield the side light-receiving probe 6, the Raman spectrum and signal intensity of the silicon carbide crystal (smooth surface sheet) were tested using the front light-receiving probe 5. Figure 11The Raman spectrum and signal intensity of a silicon carbide crystal (smooth, sheet-like surface) were measured together by the front-facing light-receiving probe 5 and the side-facing light-receiving probe 6. Based on Figures 9-11 It can be seen that with the addition of the side light-receiving probe 6, the signal of the silicon carbide crystal (smooth, sheet-like surface) is enhanced by approximately 1.52-1.67 times compared to having only the front light-receiving probe 5.

[0103] Finally, by comparing the test data of diamond powder samples with those of silicon carbide crystals (smooth, plate-like surfaces), it can be concluded that, especially for rough diamond powder, the overall Raman signal enhancement ratio is higher than that of silicon carbide crystals (smooth, plate-like surfaces), further verifying that the present invention can improve the Raman signal intensity and detection capability of rough samples.

[0104] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on its differences from other embodiments. Similar or identical parts between embodiments can be referred to interchangeably. Those skilled in the art will further recognize that the units and algorithm steps of the examples described in conjunction with the embodiments disclosed herein can be implemented in electronic hardware, computer software, or a combination of both. To clearly illustrate the interchangeability of hardware and software, the composition and steps of each example have been generally described in terms of functionality in the foregoing description. Whether these functions are implemented in hardware or software depends on the specific application and design constraints of the technical solution. Those skilled in the art can use different methods to implement the described functions for each specific application, but such implementation should not be considered beyond the scope of this application.

[0105] The sample testing system and method provided in this application have been described in detail above. Specific examples have been used to illustrate the principles and implementation methods of this application. The descriptions of the embodiments above are only for the purpose of helping to understand the method and its core ideas. It should be noted that, based on the embodiments in this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application. Several improvements and modifications can be made to this application without departing from the principles of this application, and these improvements and modifications also fall within the scope of protection of this application.

Claims

1. A sample detection system, characterized by, It includes a light projection module, a sample carrying device, a signal acquisition module, and a sample detection module; The light projection module is used to emit a laser signal of the target wavelength and focus the laser signal onto the sample to be tested on the sample carrier device; The signal acquisition module includes a front light-receiving probe and at least one side light-receiving probe for acquiring the scattered light signal generated by the sample under test. The scattered light signal includes Raman scattering and Rayleigh scattering signals. The front light-receiving probe is deployed in the forward optical path of the scattered light signal from the sample under test, and the axis of the front light-receiving probe is perpendicular to the surface of the sample support device. The side light-receiving probes are deployed in the respective lateral optical paths of the scattered light signal from the sample under test. The sample detection module is used to perform spectral composition analysis on the scattered light signal of the sample to be tested to obtain the Raman scattering spectrum, and to detect the sample to be tested based on the Raman scattering spectrum; The signal acquisition module includes a first side light-receiving probe, a second side light-receiving probe, and a third side light-receiving probe; the area covered by the scattered light emitted from the side of the focused spot on the surface of the sample to be tested is divided into a first region, a second region, and a third region. The first side-mounted light-collecting probe is deployed at a first target position within a first area covered by the scattered light emitted from the side of the focused light spot on the surface of the sample to be tested, in order to collect the scattered light within the first area; The second side-mounted light-collecting probe is deployed at a second target position in a second region covered by the scattered light emitted from the side of the focused light spot on the surface of the sample to be tested, in order to collect the scattered light within the second region; The third side-mounted light-collecting probe is deployed at the third target position in the third region covered by the scattered light emitted from the side of the focused light spot on the surface of the sample to be tested, in order to collect the scattered light in the third region. The mounting base for the side-mounted light-receiving probe is a multi-functional movable base; The side light-receiving probe adjusts its spatial position during the horizontal and vertical movement of the multifunctional movable base, and adjusts its acquisition angle during the rotation of the multifunctional movable base.

2. The sample detection system according to claim 1, characterized in that, The light projection module includes a laser, a beam splitter, and a focusing element; The laser emits a laser signal at the target wavelength; The beam splitter reflects the laser signal of the target wavelength to the focusing element to obtain the reflected light signal; The focusing element focuses the reflected light signal onto the sample to be tested and collimates the forward scattered light signal of the sample to be tested. The collimated light signal enters the front light receiving probe through the beam splitting element. The beam of the front light-receiving probe is collimated axially with the focusing element and coaxial with the center of the scattered light beam refracted by the beam-splitting element.

3. The sample detection system of claim 1, wherein, The light projection module includes a monochromatic laser, a dichroic mirror, and a laser focusing lens; The monochromatic laser emits a monochromatic laser signal of the target wavelength; The dichroic mirror is matched with the target wavelength, reflecting light signals less than or equal to the target wavelength to the laser focusing lens, and transmitting light signals greater than the target wavelength. The laser focusing lens focuses the reflected light signal into a focused spot and collimates the scattered light emitted from the front of the focused spot; The front-facing light-receiving probe is axially collimated with the laser focusing lens, and the beam center of the scattered light refracted by the dichroic mirror is coaxial; the focused light spot is located on the surface of the sample to be tested.

4. The sample detection system of claim 2, wherein, The front-facing light-receiving probe includes, in sequence according to the direction of scattered light signal propagation, a first long-pass filter, a first focusing lens, and a first optical fiber; The scattered light emitted from the front of the focused spot of the focusing element is collimated, passes through the beam splitter, is projected onto the first long-pass filter, and is then focused by the first focusing lens onto the entrance of the first optical fiber.

5. The sample detection system of claim 1, wherein, The sample detection module includes an optical signal transmission element, a spectrometer, and a computing device; The signal acquisition module is connected to the spectrometer through the optical signal transmission element. The spectrometer performs spectral composition analysis on the scattered light signal of the sample to be tested and sends the spectral analysis data to the computing device. The computing device generates Raman scattering spectra based on the spectral analysis data.

6. The sample detection system of claim 1, wherein, The sample carrying device includes a retractable and movable base; During the horizontal and / or vertical movement of the retractable movable base, the sample carrier device adjusts its spatial position.

7. The sample testing system according to any one of claims 1 to 6, characterized in that, The side-mounted light-receiving probe includes, in sequence according to the direction of scattered light signal propagation, a collimating lens, a second long-pass filter, a second focusing lens, and a second optical fiber; The focal point of the collimating lens is located at the focused spot on the surface of the sample to be tested. The collimating lens collimates the scattered light emitted from the side of the focused spot and projects it onto the second long-pass filter, which is then focused by the second focusing lens onto the entrance of the second optical fiber. The collimating lens has the same numerical aperture as the focusing element of the light projection module.

8. A sample detection method, characterized by, The sample detection system as described in any one of claims 1 to 7 comprises: When the sample to be tested is detected to be placed in the sample placement area of ​​the sample carrier, a laser signal of the target wavelength is emitted and the laser signal is focused onto the sample to be tested. Raman scattering spectrum is generated based on the spectral composition analysis data of the Raman scattering and Rayleigh scattering signals generated by the sample under test.

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