Major QTL locus QYp-7D-1 for yellow pigment content in wheat grains and its SNP molecular marker and its application

Abstract

The invention provides a major effect QTL site QYp‑7D‑1 of yellow pigment content in wheat grains and a SNP molecular marker and application thereof, and the invention belongs to the technical field of wheat breeding. The SNP molecular marker Marker473755 is located on chromosome 7D of wheat, and the nucleotide sequence of the molecular marker is shown in SEQ ID NO.3 or SEQ ID NO.4; the SNP molecular marker Marker473755 is located on chromosome 7D of wheat, and the nucleotide sequence of the molecular marker is shown in SEQ ID NO.3 or SEQ ID NO.4; the SNP site of the invention is of great significance for detecting varieties, strains and breeding materials with low yellow pigment in wheat grains, and molecular markers can be developed based on the SNP site to accelerate the efficiency of wheat quality breeding.

Description

Technical Field

[0001] The invention belongs to the technical field of wheat breeding, and particularly relates to a major effect QTL locus QYp-7D-1 of yellow pigment content in wheat grains and a SNP molecular marker and application thereof. Background Art

[0002] There are various pigments in wheat flour, which are related to the quality of wheat flour products, such as yellow pigments, brown pigments, etc. (Zheng Wenyin, 2013). Yellow pigments mainly include carotenoids, lutein, esters, etc., and there are very small amounts of anthocyanins and flavonoids (Belitz et al., 1987). Studies have found that the yellow pigment content in wheat grains is highly positively correlated with the yellowness of flour and dough, with a correlation coefficient higher than 0.8, and some can even reach 0.9 (Lan Jing et al., 2004). The yellow pigment content is extremely significantly negatively correlated with the whiteness of flour, and is significantly negatively correlated with various noodle processing quality traits. At the same time, it is also negatively correlated with the quality traits of Chinese noodles such as palatability and stickiness (Kruger, 1992; Yang Jin et al., 2004; Hu Ruibo et al., 2004). In the eating habits of most people today, the color of noodle products affects the sales and consumption of flour. Therefore, reducing the yellow pigment content also plays a very important role in improving wheat quality (He, 2004). Previous studies have shown that although the environment has a certain influence on the yellow pigment content (Miskelly, 1984), the most important influence is still the gene; other studies have found that in the hybrid combination of two hard wheats, the first generation showed a dominant low yellow pigment level, while the yellow pigment of the next two generations was more inclined to the average level of the parents. There are few wheat varieties with excellent alleles with low yellow pigment content in the Huanghuai wheat region. These varieties contain low yellow pigment content gene combinations, which play an important role in reducing the yellow pigment content in grains. Based on the location research of yellow pigment, predecessors have developed multiple markers for detecting high and low yellow pigment content. For example, YP7A can distinguish the 7A chromosome yellow pigment alleles Psy-A1a (high YP content, 194bp) and Psy-A1b (low YP content, 231bp), and the marker YP7A-2 can distinguish Psy-A1a / b (1686bp) and Psy-A1c (1001bp). There are also markers on chromosome 7B that can distinguish the alleles of yellow pigment content. YP7B-1 and YP7B-2 can distinguish the three alleles of Psy-B1a (medium-high YP content, 151bp), Psy-B1b (low YP content, 156bp) and Psy-B1c (high YP content, 428bp). At the same time, there are two markers that can distinguish the yellow pigment alleles on chromosome 7D, namely YP7D-1 and YP7D-2, which mainly distinguish Psy-D1a and Psy-D1g (Hu Fengling et al., 2012; Yang Jishu, 2014; Sun Jianxi, 2014; Wang Zhongxing, 2016; Chen Ling et al., 2017).

[0003] Parker et al. (1998) found a major QTL related to yellow pigment content on chromosome 7A, which was closely linked to AFLP marker Xwua26-7A.4. Mares and Campbell (2001) used DH population to study and found that there were also major QTLs on chromosomes 7A and 3B. Some predecessors also found major loci for yellow pigment on chromosomes 7A and 7B (Zhang Liping, 2005; Zhang Liping, 2006; Elouafi, 2001). Similarly, Pozniak et al. (2007) located 4 QTLs in durum wheat, located on 2A, 4B, 6B, and 7B. A study used RIL population (Xingcheng 8901×Zhoumai 16) to locate QTLs for yellow pigment content in two different environments, and located them on eight chromosomes, namely 2A, 2B, 2D, 5A, 5B, 6B, 7A, and 7B. In this study, a RIL population with different grain yellow pigment traits constructed from the maternal Nongda Nuowan No. 1 and the paternal Gaocheng 8901 wheat was used as the material. Combined with the high-density genetic map constructed using the SLAF-Seq technology, QTL positioning analysis of grain yellow pigment content was carried out under different environments, in order to identify the major QTL loci and their SNP markers, and provide genetic resources and molecular markers for controlling the yellow pigment content in wheat. Summary of the invention

[0004] In view of the deficiencies in the prior art, the present invention provides a major QTL locus QYp-7D-1 for yellow pigment content in wheat grains and its SNP molecular marker and application.

[0005] The present invention uses a RIL population constructed from the female parent Nongda glutinous wheat No. 1 and the male parent Gaocheng 8901 wheat as materials, and uses SLAF-Seq technology to construct a high-density genetic map containing 8,095 SNP markers. The total length of the map is 2,554.10 cM, and the linkage group length is 90.08-151.67 cM.

[0006] Combined with the results of quantitative trait gene positioning analysis, and through excellent allele identification and gene locus phenotypic analysis, a major and tightly linked QTL locus QYp-7D-1 controlling the yellow pigment content in grains was identified. It is located on chromosome 7D and has two tightly linked SNP markers, SNP Marker473757 and Marker473755. This provides theoretical guidance and basis for the discovery of wheat grain yellow pigment genes and molecular marker-assisted quality selection breeding.

[0007] The technical solution of the present invention is as follows:

[0008] A SNP molecular marker Marker473757 related to controlling the yellow pigment content of wheat grains is located on chromosome 7D of wheat. The nucleotide sequence of the molecular marker is shown in SEQ ID NO.1 or SEQ ID NO.2.

[0009] The nucleotide sequence of the molecular marker is shown in Table 8: Sequence information of grain yellow pigment SNP site.

[0010] The physical position of the SNP molecular marker Marker473757 is 90416345 of chromosome 7D, and the base changes from G to C.

[0011] Preferably according to the present invention, the nucleotide sequence of the SNP molecular marker Marker473757 is as shown in SE Q ID NO.2, that is, the wheat grains with base type C at the SNP site have low yellow pigment content.

[0012] The base variation type and phenotypic value of the SNP molecular marker are shown in Table 7: Phenotypic effect of base variation at grain yellow pigment SNP site, that is, the wheat grain yellow pigment content is low when the base type of the sequence at this position is C.

[0013] Preferably according to the present invention, the nucleotide sequence of the SNP molecular marker Marker473757 is as shown in SEQ ID NO.2, that is, the base type of the SNP site is C, and the genotype of the SNP molecular marker is CC; the nucleotide sequence of the SNP molecular marker Marker473757 is as shown in SEQ ID NO.1, that is, the base type of the SNP site is G, and the genotype of the SNP molecular marker is GG; the yellow pigment content in wheat grains of individuals with the CC genotype of the SNP molecular marker is lower than that of individuals with the GG genotype.

[0014] The base variation type and phenotypic value of the SNP molecular marker are shown in Table 7: Phenotypic effect of base variation at grain yellow pigment SNP site, that is, after the base variation occurs, the genotype of the sample where the SNP molecular marker is located is CC type, and the wheat grain yellow pigment content of individuals with CC genotype of the SNP molecular marker is lower than that of individuals with GG genotype.

[0015] A SNP molecular marker Marker473755 related to controlling the yellow pigment content of wheat grains is located on chromosome 7D of wheat. The nucleotide sequence of the molecular marker is shown in SEQ ID NO.3 or SEQ ID NO.4.

[0016] The nucleotide sequence of the molecular marker is shown in Table 8: Sequence information of grain yellow pigment SNP site.

[0017] The physical position of the SNP molecular marker Marker473755 is 90288951 of chromosome 7D, and the base changes from G to T.

[0018] Preferably according to the present invention, the nucleotide sequence of the SNP molecular marker Marker473755 is as shown in SE Q ID NO.4, that is, the wheat grains with the base type of the SNP site being T have low yellow pigment content.

[0019] The base variation type and phenotypic value of the SNP molecular marker are shown in Table 7: Phenotypic effect of base variation at grain yellow pigment SNP site, that is, the wheat grain yellow pigment content is low when the base type of the sequence at this position is T.

[0020] Preferably according to the present invention, the nucleotide sequence of the SNP molecular marker Marker473755 is as shown in SEQ ID NO.4, that is, the base type of the SNP site is T, and the genotype of the SNP molecular marker is TT; the nucleotide sequence of the SNP molecular marker Marker473755 is as shown in SEQ ID NO.3, that is, the base type of the SNP site is G, and the genotype of the SNP molecular marker is GG; the yellow pigment content in wheat grains of individuals with the SNP molecular marker TT genotype is lower than that of individuals with the GG genotype.

[0021] The base variation type and phenotypic value of the SNP molecular marker are shown in Table 7: Phenotypic effect of base variation at the grain yellow pigment SNP site, that is, after the base variation occurs, the genotype of the sample where the SNP molecular marker is located is TT type, and the wheat grain yellow pigment content of individuals with the TT genotype of the SNP molecular marker is lower than that of individuals with the GG genotype.

[0022] A use of SNP molecular markers in breeding wheat varieties or strains with low yellow pigment content in wheat grains, wherein the SNP molecular markers include one or more of the above-mentioned SNP molecular markers Marker 473757 and Marker 473755.

[0023] According to a preferred embodiment of the present invention, in the use, the nucleotide sequence of the SNP molecular marker Marker473757 is as shown in SEQ ID NO.2, that is, the wheat grains with the base type C of the SNP site have low yellow pigment content;

[0024] The nucleotide sequence of the SNP molecular marker Marker473757 is shown as SEQ ID NO.2, that is, the base type of the SNP site is C, and the genotype of the SNP molecular marker is CC; the nucleotide sequence of the SNP molecular marker Marker473757 is shown as SEQ ID NO.1, that is, the base type of the SNP site is G, and the genotype of the SNP molecular marker is GG; the yellow pigment content in wheat grains of individuals with the CC genotype of the SNP molecular marker is lower than that of individuals with the GG genotype.

[0025] According to a preferred embodiment of the present invention, in the use, the nucleotide sequence of the SNP molecular marker Marker473755 is as shown in SEQ ID NO.4, that is, the wheat grains with the base type of the SNP site being T have low yellow pigment content;

[0026] The nucleotide sequence of the SNP molecular marker Marker473755 is shown as SEQ ID NO.4, that is, the base type of the SNP site is T, and the genotype of the SNP molecular marker is TT; the nucleotide sequence of the SNP molecular marker Marker473755 is shown as SEQ ID NO.3, that is, the base type of the SNP site is G, and the genotype of the SNP molecular marker is GG; the yellow pigment content in wheat grains of individuals with the SNP molecular marker TT genotype is lower than that of individuals with the GG genotype.

[0027] Preferably, according to the present invention, in the use, wheat whose SNP site base type of SNP molecular marker Marker473757 is C and whose SNP site base type of SNP molecular marker Marker473755 is T is bred;

[0028] The wheat whose genotype of SNP molecular marker Marker473757 is CC type and whose genotype of SNP molecular marker Marker473755 is TT type was selected.

[0029] A method for screening wheat grains with low yellow pigment content, using one or more of the above-mentioned SNP molecular markers Marker473757 and Marker473755 for screening.

[0030] Preferably, in the method, the nucleotide sequence of the SNP molecular marker Marker473757 is as shown in SEQ ID NO.2, that is, the wheat grains with base type C at the SNP site have low yellow pigment content;

[0031] The nucleotide sequence of the SNP molecular marker Marker473757 is shown as SEQ ID NO.2, that is, the base type of the SNP site is C, and the genotype of the SNP molecular marker is CC; the nucleotide sequence of the SNP molecular marker Marker473757 is shown as SEQ ID NO.1, that is, the base type of the SNP site is G, and the genotype of the SNP molecular marker is GG; the yellow pigment content in wheat grains of individuals with the CC genotype of the SNP molecular marker is lower than that of individuals with the GG genotype.

[0032] Preferably, in the method, the nucleotide sequence of the SNP molecular marker Marker473755 is as shown in SEQ ID NO.4, that is, the wheat grains with the base type of the SNP site being T have low yellow pigment content;

[0033] The nucleotide sequence of the SNP molecular marker Marker473755 is shown as SEQ ID NO.4, that is, the base type of the SNP site is T, and the genotype of the SNP molecular marker is TT; the nucleotide sequence of the SNP molecular marker Marker473755 is shown as SEQ ID NO.3, that is, the base type of the SNP site is G, and the genotype of the SNP molecular marker is GG; the yellow pigment content in wheat grains of individuals with the SNP molecular marker TT genotype is lower than that of individuals with the GG genotype.

[0034] Preferably, according to the present invention, in the method, wheat whose base type of the SNP site of SNP molecular marker Marker473757 is C and whose base type of the SNP site of SNP molecular marker Marker473755 is T is screened;

[0035] The wheat whose genotype of SNP molecular marker Marker473757 is CC type and whose genotype of SNP molecular marker Marker473755 is TT type was screened.

[0036] The SNP molecular marker Marker473757 is located on the wheat chromosome 7D. The nucleotide sequences of 300 bp before and after the molecular marker are shown in Table 8: Sequence information of grain yellow pigment SNP site. The base of the SNP molecular marker Marker473757 changes from G to C.

[0037] The SNP molecular marker Marker473755 is located on wheat chromosome 7D. The nucleotide sequences of 300 bp before and after the molecular marker are shown in Table 8: Grain yellow pigment SNP site sequence information. The base of the SNP molecular marker Marker473755 changes from G to T.

[0038] Beneficial effects of the present invention

[0039] The SNP site provided by the present invention is of great significance for detecting and breeding wheat grain low yellow pigment varieties, strains, and breeding materials. Molecular markers can be developed based on the SNP site to accelerate the efficiency of wheat quality breeding. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] Figure 1 This is the frequency distribution diagram of the average yellow pigment content in the grains of the groups under three environments.

[0041] Figure 2 This is the genetic map result. DETAILED DESCRIPTION

[0042] The technical scheme of the present invention is further described below in conjunction with the embodiments and the accompanying drawings, but the protection scope of the present invention is not limited thereto. The reagents and drugs involved in the embodiments, unless otherwise specified, are all common commercially available products; the experimental operations involved in the embodiments, unless otherwise specified, are all routine operations in the art.

[0043] 1. Materials and Methods

[0044] 1.1 Plant materials

[0045] The experimental materials are a RIL population of 268 stable homozygous families obtained by hybridizing Gaocheng 8901 (male parent) and Nongda Nuomai No. 1 (female parent) through single-grain transmission. Nongda Nuomai No. 1 is a full-glutinous wheat variety bred by hybridizing "Jiangsu Baihuomai" and "Guandong 107"; Gaocheng 8901 is a high-quality strong-gluten wheat variety bred by hybridizing "77546-2" and "Linzhang".

[0046] The RIL population and parents were planted in the experimental field of Shandong Agricultural University in Tai'an under the 2017-2018 environment (E1) and the 2018-2019 environment (E2), with a randomized block design; and were planted in the Wenyang experimental field in Tai'an under the 2019-2020 environment (E3), with a randomized block design; both were repeated 3 times; and the field management was the same as the local field management.

[0047] According to local conventional methods, no serious pests and lodging occurred during the growth period. When mature, the fruit was harvested by plot, dried and stored indoors according to standards.

[0048] 1.2 Experimental methods

[0049] 1.2.1 Phenotypic identification of yellow pigment content in grains

[0050] The determination of yellow pigment is based on AACC14-50 with slight modifications. Weigh 1g of whole wheat flour into a 10mL centrifuge tube, add 5mL of water-saturated n-butanol, shake and extract on a reciprocating shaker for 1h (37℃, 200r / min), then centrifuge (4000r / min, 10min), take the supernatant, measure its absorbance at a wavelength of 440nm, and do three sets of parallel tests. The measured absorbance value is multiplied by a constant of 30.1, and the value obtained is the yellow pigment content (mg / kg). Each sample is repeated 3 times and the average value is taken.

[0051] 1.2.2 SLAF library construction and SNP marker detection

[0052] The wheat genome was selected as the reference genome. After enzyme prediction, the optimal endonuclease was finally determined to be HaeIII. The sequence with a length of 464-494bp was defined as the SLAF tag, and the SLAF tag data was predicted. The filtered sequencing data was analyzed for GC content and Q30, as well as the data volume. The results of the control data were monitored using Nipponbare rice as a control to prove the correctness of the experimental process and determine whether the enzyme cutting scheme was effective. According to the positioning results of the sequencing reads on the reference genome, GATK performed local realignment, GATK variation detection, samtools variation detection, and the intersection variation sites of the results of the two methods of GATK and samtools were taken to ensure the accuracy of the obtained SNPs, and the final SNP site set was obtained, as shown in Table 1. Among them, the aaxbb type is suitable for the RIL population in this experiment, and the remaining markers are suitable for hybrid populations.

[0053] Table 1 SNP genotype coding rules

[0054]

[0055]

[0056] 1.2.3 High-density genetic map construction and QTL positioning

[0057] In order to ensure the quality of the genetic map, polymorphic SNP tags were filtered, and polymorphic markers with severe skewed separation (chi-square test P < 0.01) were filtered according to the standard. The SNP tags that were screened were filtered by calculating the MLOD value between the two tags of the filtered SNP data, setting the number of tags for the minimum and maximum groups, and arranging the tags according to the MLOD value of the preset MLOD value interval from small to large. The tags with the highest MLOD value between the tags will be divided into a linkage group, and the tags with MLOD values ​​lower than 5 with other SNP tags will be filtered out, and finally positioned as mapping markers (Marker). Taking the linkage group as the unit, HighMap software was used to analyze the linear arrangement of the markers in the linkage group, and then the genetic distance between the two adjacent markers was calculated to obtain the final genetic map. At the same time, the single source evaluation was carried out: the single source of each sample in all linkage groups was counted to find possible double exchange sites. In a linkage group, double exchange is usually controlled below 3%, and linkage relationship evaluation is also required. The closer the distance between markers, the lower the recombination rate. Linkage analysis was performed on the data from the RIL population using QTL IciMapping software and R / QTL mapping software. QTL naming was based on the method of McIntosh et al. (2005).

[0058] 1.2.4 Candidate gene prediction

[0059] The quantitative trait QTL loci with a high stable contribution rate to the grain yellow pigment trait composition were selected as candidate regions for functional annotation. The wheat genome sequence on the public data platform of BioMed Company was used as the reference genome sequence. The located QTL locus region was aligned to the reference genome scaffold sequence using BLAST software, and the matching candidate interval was aligned with the published wheat template to obtain the corresponding coding gene information, such as genes. In addition, a BLAST (Basic Local Alignment Search Tool) search was performed on the International Wheat Genome Sequencing Consortium database (IWGSC; http: / / www.wheatgenome.org / ). When the SNP marker sequence was 100% identical to any wheat overlap group, the sequence of each marker was extended by 5000bp using the IWGSC BLAST result. Then, the extended sequence was used to search the database of the National Center for Biotechnology Information (NCBI) (http: / / www.ncbi.nlm.nih.gov) and Ensembl Plants (http: / /

[0060] plant.ensembl.org / Triticum_aestivum / Tools / Blast) was used to identify possible candidate genes and functions.

[0061] (Note: Wheat reference genome download address:

[0062] http: / / www.wheatgenome.org / News / Latest-news / IWGSC-Reference-Sequence-v1.0-browser-now-available-at-URGI; Download address of Nipponbare rice genome:

[0063] http: / / rapdb.dna.affrc.go.jp)

[0064] 2. Results

[0065] 2.1 Phenotypic analysis of yellow pigment content in wheat grains of RIL population

[0066] The two parents of the RIL population differed in yellow pigment content. The phenotypic variation of yellow pigment content in the grains of the RIL population was large, and there was obvious super-parent separation (Table 2). The maximum yellow pigment content of wheat grains in the three environments was 6.50, which appeared in the E1 environment, and the minimum was 1.80, which appeared in the E2 environment. The average value was 3.12-4.61, and the coefficient of variation was 15.32%-17.32%. The population separation was continuous and conformed to the normal distribution ( Figure 1 ), showing typical quantitative traits.

[0067] Table 2 Phenotypic analysis of wheat grain yellow pigment characters in RIL population

[0068]

[0069] Note: E1: 2017-2018 Taian, E2: 2018-2019 Taian, E3: 2019-2020 Taian

[0070] YP: Yellow pigment content in seeds

[0071] 2.2 Graph Information

[0072] After a systematic and rigorous screening, 13,658 SNP tags were finally obtained for mapping, and the linkage grouping is shown in Table 3. The MLOD values ​​of the 13,658 SNP tags were calculated between each tag, and the SNP tags with MLOD values ​​lower than 5 were filtered out. A total of 8,095 SNP tags were mapped, with a mapping rate of 59.27%.

[0073] Table 3Marker linkage grouping

[0074]

[0075]

[0076] Taking linkage groups as units, HighMap software was used to analyze the linear arrangement of markers within linkage groups and estimate the genetic distances between adjacent markers. Figure 2 As shown, the final genetic map with a total map distance of 2,554.10 cM was obtained (Table 4).

[0077] Table 4 Map information

[0078]

[0079] 2.3 QTL analysis of yellow pigment content in wheat grains

[0080] Through linkage analysis of wheat grain yellow pigment content (Table 5), four QTL loci related to grain yellow pigment traits were detected, namely QYp-4B, QYp-3D, QYp-7D-1, and QYp-7D-2. The LOD values ​​were 3.0, 2.7, 5.2, and 3.0, respectively, the additive effects were -0.14, 0.10, -0.16, and -0.12, respectively, and the phenotypic contribution rates were 11.0%, 10.2%, 17.0%, and 4.5%, respectively. QYp-7D-1 was detected in both E2 and E3 environments.

[0081] Table 5 QTL loci of YR traits in wheat grain of RIL population

[0082]

[0083] Note: E1: 2017-2018 Taian, E2: 2018-2019 Taian, E3: 2019-2020 Taian

[0084] As shown in Table 6, there are two SNP sites, Marker473757 and Marker473755, in the main QTL locus QYp-7D-1 on chromosome 7D that controls the stability of grain yellow pigment content, and their base variations are associated with the grain yellow pigment content.

[0085] Table 6 QTL and SNP markers for grain YP traits of wheat in RIL population

[0086]

[0087] As shown in Table 7, the Marker 473757 marker is located on the wheat chromosome 7D, and the base C is an excellent variant base; the Marker 473755 marker is located on the wheat chromosome 7D, and the base T is an excellent variant base. The SNP site sequence information of each marker is shown in Table 8.

[0088] Table 7 Phenotypic effect of SNP base variation of wheat grain YP traits

[0089]

[0090] Table 8 SNP locus sequence information of grain YP traits

[0091]

[0092]

[0093] As shown in Table 9, the most excellent haplotype of the two SNP markers that affect the YP activity of the grain is CT, and the non-excellent haplotype is GG. As the number of excellent alleles of the SNP marker site increases, the YP content of the grain gradually decreases. When the two SNP markers are both non-excellent base variation haplotype GG, the grain YP content value is higher>4.45; when one of the two SNP markers is an excellent base variation, the grain YP content decreases; when the variation of the two SNP markers is both excellent allele CT, the grain YP content is greatly reduced, and the lowest can reach 2.40. Therefore, when the haplotype combination is GG, the grain YP content value is high, and when the excellent haplotype combination is CT, the grain YP content value is low, and the number of excellent variation sites has a cumulative effect on the grain YP content value. Therefore, these two SNP sites play an important role in the YP content of wheat grains.

[0094] Table 9 Haplotype statistics of base variation of YP content sites in grains

[0095] Table 9Haplotypes of yellow pigment content with different basevariation in wheat

[0096] grain

[0097]

[0098] 2.4 Prediction of candidate genes for major loci of yellow pigment content in wheat grains

[0099] Candidate genes for the major stable QTL locus QYp-7D-1 were predicted, as shown in Table 10. Through gene comparison, 22 candidate genes were predicted for the major QTL locus QYp-7D-1 on chromosome 7D that controls the stability of grain yellow pigment, and their functions mainly involved GDP-mannose transporter, wheat F-Box protein, hexosyltransferase, ubiquitin carboxyl terminal hydrolase, 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily proteins.

[0100] Table 10 Qyp-7D-1 candidate gene prediction

[0101]

[0102]

[0103] The SNP site provided by the present invention is of great significance for detecting and breeding wheat grain low yellow pigment varieties, strains, and breeding materials. Molecular markers can be developed based on the SNP site to accelerate the efficiency of wheat quality breeding.

Claims

1. A SNP molecular marker Marker473757 related to controlling the yellow pigment content of wheat grains, which is located on chromosome 7D of wheat. The nucleotide sequence of the molecular marker is shown in SEQ ID NO.1 or SEQ ID NO.

2.

2. The molecular marker according to claim 1, characterized in that The nucleotide sequence of the SNP molecular marker Marker473757 is shown in SEQ ID NO.2, that is, the wheat grains with the base type C of the SNP site of Marker473757 have low yellow pigment content.

3. The molecular marker according to claim 1, characterized in that The nucleotide sequence of the SNP molecular marker Marker473757 is shown as SEQ ID NO.2, that is, the base type of the SNP site of Marker473757 is C, and the genotype of the SNP molecular marker is CC; the nucleotide sequence of the SNP molecular marker Marker473757 is shown as SEQ ID NO.1, that is, the base type of the SNP site of Marker473757 is G, and the genotype of the SNP molecular marker is GG; the yellow pigment content in wheat grains of individuals with the CC genotype of the SNP molecular marker is lower than that of individuals with the GG genotype.

4. A SNP molecular marker Marker473755 related to controlling the yellow pigment content in wheat grains, which is located on chromosome 7D of wheat. The nucleotide sequence of the molecular marker is shown in SEQ ID NO.3 or SEQ ID NO.

4.

5. The molecular marker according to claim 4, characterized in that The nucleotide sequence of the SNP molecular marker Marker473755 is shown in SEQ ID NO.4, that is, the wheat grains with the base type T of the SNP site of Marker473755 have low yellow pigment content.

6. The molecular marker according to claim 4, characterized in that The nucleotide sequence of the SNP molecular marker Marker473755 is shown as SEQ ID NO.4, that is, the base type of the SNP site of Marker473755 is T, and the genotype of the SNP molecular marker is TT; the nucleotide sequence of the SNP molecular marker Marker473755 is shown as SEQ ID NO.3, that is, the base type of the SNP site of Marker473755 is G, and the genotype of the SNP molecular marker is GG; the yellow pigment content in wheat grains of individuals with the SNP molecular marker TT genotype is lower than that of individuals with the GG genotype.

7. A use of SNP molecular markers in breeding wheat varieties or strains with low yellow pigment content in wheat grains, wherein the SNP molecular markers include one or more of the SNP molecular marker Marker 473757 described in any one of claims 1 to 3 and the SNP molecular marker Marker 473755 described in any one of claims 4 to 6.

8. The use according to claim 7, characterized in that The nucleotide sequence of the SNP molecular marker Marker473757 is shown in SEQ ID NO.2, that is, the wheat grains with the base type C of the SNP site of Marker473757 have low yellow pigment content; The nucleotide sequence of the SNP molecular marker Marker473757 is shown as SEQ ID NO.2, that is, the base type of the SNP site of Marker473757 is C, and the genotype of the SNP molecular marker is CC; the nucleotide sequence of the SNP molecular marker Marker473757 is shown as SEQ ID NO.1, that is, the base type of the SNP site of Marker473757 is G, and the genotype of the SNP molecular marker is GG; the yellow pigment content in wheat grains of individuals with the CC genotype of the SNP molecular marker is lower than that of individuals with the GG genotype.

9. The use according to claim 7, characterized in that The nucleotide sequence of the SNP molecular marker Marker473755 is shown in SEQ ID NO.4, that is, the wheat grains with the base type of the SNP site of Marker473755 being T have low yellow pigment content; The nucleotide sequence of the SNP molecular marker Marker473755 is shown as SEQ ID NO.4, that is, the base type of the SNP site of Marker473755 is T, and the genotype of the SNP molecular marker is TT; the nucleotide sequence of the SNP molecular marker Marker473755 is shown as SEQ ID NO.3, that is, the base type of the SNP site of Marker473755 is G, and the genotype of the SNP molecular marker is GG; the yellow pigment content of wheat grains of individuals with the SNP molecular marker TT genotype is lower than that of individuals with the GG genotype.

10. The use according to claim 7, characterized in that Breed wheat with the base type of the SNP site of SNP molecular marker Marker473757 being C and the base type of the SNP site of SNP molecular marker Marker473755 being T; The wheat whose genotype of SNP molecular marker Marker473757 is CC type and whose genotype of SNP molecular marker Marker473755 is TT type was selected.

11. A method for screening wheat grains with low yellow pigment content, characterized in that: Screening is performed using one or more of the SNP molecular marker Marker 473757 described in any one of claims 1 to 3 and the SNP molecular marker Marker 473755 described in any one of claims 4 to 6.

12. The method according to claim 11, characterized in that The nucleotide sequence of the SNP molecular marker Marker473757 is shown in SEQ ID NO.2, that is, the wheat grains with the base type C of the SNP site of Marker473757 have low yellow pigment content; The nucleotide sequence of the SNP molecular marker Marker473757 is shown as SEQ ID NO.2, that is, the base type of the SNP site of Marker473757 is C, and the genotype of the SNP molecular marker is CC; the nucleotide sequence of the SNP molecular marker Marker473757 is shown as SEQ ID NO.1, that is, the base type of the SNP site of Marker473757 is G, and the genotype of the SNP molecular marker is GG; the yellow pigment content in wheat grains of individuals with the CC genotype of the SNP molecular marker is lower than that of individuals with the GG genotype.

13. The method according to claim 11, characterized in that The nucleotide sequence of the SNP molecular marker Marker473755 is shown in SEQ ID NO.4, that is, the wheat grains with the base type of the SNP site of Marker473755 being T have low yellow pigment content; The nucleotide sequence of the SNP molecular marker Marker473755 is shown as SEQ ID NO.4, that is, the base type of the SNP site of Marker473755 is T, and the genotype of the SNP molecular marker is TT; the nucleotide sequence of the SNP molecular marker Marker473755 is shown as SEQ ID NO.3, that is, the base type of the SNP site of Marker473755 is G, and the genotype of the SNP molecular marker is GG; the yellow pigment content of wheat grains of individuals with the SNP molecular marker TT genotype is lower than that of individuals with the GG genotype.

14. The method according to claim 11, characterized in that In the method, wheat whose base type of the SNP site of SNP molecular marker Marker473757 is C and whose base type of the SNP site of SNP molecular marker Marker473755 is T is screened; The wheat whose genotype of SNP molecular marker Marker473757 is CC type and whose genotype of SNP molecular marker Marker473755 is TT type was screened.

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