A composition with barrier repair, soothing efficacy, its preparation method and application

Abstract

The application provides a composition with barrier repair and soothing effects, which utilizes the synergistic effect of tuberose, peony and cactus, increases the richness of active functional components in the composition, and makes the prepared composition exhibit excellent moisturizing, antioxidant, antibacterial and sterilization effects on the skin. The mass ratio of the tuberose, peony and cactus is (1-4):(0.1-0.4):(1-4), so that the active components in the composition exhibit the best synergistic effect and stability, promote the penetration of each other into the skin, stimulate the skin cells and tissues from the deep, and radically cure the skin allergic symptoms; and the composition can be used in cosmetics.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetics technology, A61K8 / 18, and in particular to a composition with barrier repair and soothing effects, its preparation method and application. BACKGROUND

[0002] The skin directly contacted with the external environment is the first barrier to maintain human health; in recent years, due to industrial or atmospheric pollution, abuse of food additives, and people's bad living habits, etc., the skin allergic phenomenon is becoming more and more serious, which makes the skin mainly show red, swollen, itchy, hot, painful, and even occasionally tight, numb, swollen, etc. These symptoms have brought great trouble to people's life.

[0003] In view of this demand, a variety of solutions have appeared on the market, such as adding hyaluronic acid, polysaccharide and other moisturizing ingredients to increase skin moisture, or using ceramide to repair the skin; although the above methods have certain effect, they all have the defect of treating the symptoms but not the root cause, and cannot realize the effect of supplementing skin moisture and treating skin allergic reaction at the same time, and the ceramide ingredient can only repair the surface tissue of the skin, and cannot treat the allergic reaction from the root.

[0004] Chinese patent CN113350260A discloses an anti-allergic acne deep cleaning skin lotion and its preparation method, which uses disaccharide moisturizing agent, salicylic acid, aloe gel freeze-dried powder, radix bletillae, bacillus subtilis and lactic acid bacteria as functional ingredients to treat skin allergic phenomenon, but its preparation method requires strict sterile conditions, and in the later use process, there is also the risk of bacterial oxidation and deterioration, reducing the repair effect. Chinese patent CN103976905B discloses a plant extract mixture with anti-inflammatory and anti-allergic effects, which improves the anti-inflammatory effect by using different plant extracts, but its anti-inflammatory and anti-allergic effect still has certain room for improvement.

[0005] Therefore, it is necessary to improve the existing soothing and repairing skin effect cosmetics to improve the anti-inflammatory and anti-allergic effect. SUMMARY

[0006] In order to solve the above technical problems, the present application first provides a composition with barrier repair and soothing effects, the raw materials of which are derived from natural substances.

[0007] Further, the natural substance has at least one of the effects of moisturizing, antioxidant and antibacterial.

[0008] Further, the natural substances with the moisturizing efficacy include, but are not limited to, at least one of American avav, Cymbopogan flexuosus, Aloes Aloe, Semen Trigonellae, Matricaria recutita, Salvia miltiorrhiza Bge., Olea europaea, tremella, and Avena sativa L.

[0009] Preferably, the natural substances with the moisturizing efficacy are selected from at least one of Aloes, Matricaria recutita, Olea europaea, and tremella.

[0010] Further, the natural substances with the antioxidant efficacy include, but are not limited to, at least one of Perillae Herba, Cortex Phellodendri Chinensis, Oenothera biennis L., losmarinus, Dendrobium nobile, Camellia sinensis, Polygonum Cuspidatum, Polianthes tuberosa L., Sargassum, and Vitis vinifera L.

[0011] Preferably, the natural substances with the antioxidant efficacy are selected from at least one of Oenothera biennis L., Dendrobium nobile, Camellia sinensis, Polianthes tuberosa L., and Vitis vinifera L.

[0012] Further, the natural substance with bacteriostatic efficacy includes, but is not limited to, at least one of Punica granatum L., Hamamelis molis, Radix Paeoniae Alba, Sophora flavescens, Glycyrrhiza inflata Batalin, Rhizoma Coptidis, Galla Chinensis, Artemisia carvifolia, Salvia officinalis, Camellia sinensis, Moringa Pterygosperma, Adenophora capillaris, Epiphyllum oxypetalum, and Ginkgo biloba Linn.

[0013] Preferably, the natural substance with bacteriostatic efficacy is at least one of Radix Paeoniae Alba, Adenophora capillaris, and Epiphyllum oxypetalum.

[0014] Preferably, the raw material of the composition includes a natural substance with antioxidant efficacy.

[0015] In a preferred embodiment, the natural substance with antioxidant efficacy is Tuberose.

[0016] Tuberose is a raw material for preparing perfume, but it is documented that it can clear heat and detoxify, treat abscesses, ulcerations, boils, pannus, treat acute conjunctivitis and keratitis, and relieve symptoms; in addition, tuberose extract can also increase the water content of the stratum corneum, and has a moisturizing effect.

[0017] Further, the raw material of the composition also includes a natural substance with bacteriostatic efficacy, specifically, Radix Paeoniae Alba.

[0018] Radix Paeoniae Alba is a traditional flower in China, which contains rich glycosides, terpenoids, flavonoids, and saccharides, etc., and traditional Chinese medicine records that it has the effects of anti-inflammatory and antibacterial, expanding blood vessels, lowering blood pressure and relieving pain, clearing heat and relieving convulsions, antioxidant, and anticancer, etc.

[0019] Further, the Radix Paeoniae Alba is at least one of Radix Paeoniae Alba flower, Radix Paeoniae Alba stem, and Radix Paeoniae Alba root, preferably Radix Paeoniae Alba flower and / or Radix Paeoniae Alba stem.

[0020] Further, in the raw materials of the composition, the mass ratio of the tuberose to the peony is (1-5):(0.1-0.5), and it is found in the research that the peony has better antioxidant and anti-inflammatory effects than the tuberose. To some extent, with the increase of the addition amount of the peony (i.e. peony flower or peony root), the antioxidant and anti-inflammatory effects of the composition show an increasing trend, but when the addition amount exceeds a certain range, the effects of the composition do not change obviously, and no matter how to adjust the addition ratio of the two, the antioxidant and anti-inflammatory effects of the composition cannot be enhanced.

[0021] Further, the raw materials of the composition also include epiphyllum, i.e. the composition also includes epiphyllum extract.

[0022] Epiphyllum is known as the moon beauty, and has the effects of softening the stool, removing toxins, clearing heat and relieving asthma, and strengthening the body. It also has the effects of sterilization and inhibition on the skin, and nourishing and nourishing.

[0023] It is found that the antioxidant and anti-inflammatory repair effects of the composition are obviously enhanced after the addition of epiphyllum in the composition. The analysis reason is that the active ingredients in epiphyllum can have excellent synergies with the active ingredients in tuberose and peony, and make up for the activity defects of tuberose and peony. When the addition amount of epiphyllum is increased, the antioxidant and anti-inflammatory effects of the composition are improved to some extent, but when it exceeds a certain value, it shows a downward trend, and the storage stability of the composition also has problems.

[0024] It is found that when the mass ratio of the tuberose, peony and epiphyllum in the raw materials of the composition is (1-4):(0.1-0.4):(1-4), the composition not only has excellent anti-inflammatory repair effect, but also has the best storage stability. The analysis reason is that the main active substances in tuberose include terpineol, methyl anthranilate, benzyl benzoate, etc.; the peony flower contains rich glycosides such as benzoyl paeoniflorin, oxidized paeoniflorin, 2-methoxy-5-(E)-propenyl-phenol-β-esculin, terpenoids such as paeoniflorin, and tannin compounds such as 1,2,3,6-O-tetragalloyl glucose, etc. The peony also contains rich glycosides such as adenosine, 2'-deoxyadenosine, benzyl β-D-glucopyranoside, etc. These compounds not only have different molecular chain lengths, but also have different polar groups. When they exist in the same system, the entanglement of adjacent molecular chains and the intermolecular forces such as hydrogen bond and electrostatic interaction will affect the overall state of the composition. When the content of the raw material components is too much, it will cause the imbalance of the intermolecular forces in the composition, resulting in the decrease of the stability of the composition, and even the inactivation of the effective substances. Therefore, the relative amount needs to be strictly controlled.

[0025] Secondly, the application provides a preparation method of a composition with barrier repair and soothing effects, comprising the following steps:

[0026] S1, crushing, drying and sieving the raw materials of the composition, and then mixing them according to the corresponding mass ratio to obtain mixed powder;

[0027] S2, adding an extraction solvent to the mixed powder, and performing co-extraction by microwave treatment and ultrasonic treatment, and collecting the extraction liquid;

[0028] S3, filtering, concentrating and drying the extraction liquid to obtain a dried composition;

[0029] S4, re-dissolving the dried composition in a solvent, and then filtering to obtain the composition.

[0030] Further, the step S1 adopts low-temperature drying, and the drying methods include but are not limited to one or a combination of several of oven drying, freeze drying and vacuum drying.

[0031] Further, the drying temperature in the step S1 is 35-55°C.

[0032] Further, the extraction solvent includes but is not limited to at least one of water, ethanol, propanol, isopropanol, acetone, glycerol, hexanol, hexanediol, ethyl acetate and polyethylene glycol.

[0033] Preferably, the extraction solvent is any one of ethanol, propanol, isopropanol and hexanediol.

[0034] Further, the mass ratio of the raw materials of the composition to the extraction solvent is 1:(10-30), preferably 1:(15-25).

[0035] Further, in the step S2, the microwave power is 700-1200W, and the microwave time is 5-20min.

[0036] Preferably, in the step S2, the microwave power is 800-1000W, and the microwave time is 5-15min.

[0037] Further, in the step S2, the ultrasonic power is 300-800W, and the ultrasonic time is 15-60min.

[0038] Preferably, in the step S2, the ultrasonic power is 400-650W, and the ultrasonic time is 20-45min.

[0039] Further, in the step S2, the microwave treatment and the ultrasonic treatment are performed in sequence or alternately, and the power and the overall time are controlled.

[0040] In one embodiment, step S2 is microwave treatment for 10 min, followed by ultrasonic treatment for 30 min.

[0041] In another embodiment, step S2 is microwave treatment for 5 min, followed by ultrasonic treatment for 15 min, then microwave treatment for 5 min, and finally ultrasonic treatment for 15 min.

[0042] Further, step S3 uses two-step filtration, the first step is to filter the extract after standing to remove the bulk of the insoluble raw material components, and then centrifuge at 2000-6000 rpm for 10-30 min to remove insoluble solid particles in the extract.

[0043] Further, in step S4, the solvent is at least one of water, glycerol, isopropyl alcohol, propylene glycol, butylene glycol, and hexylene glycol.

[0044] Preferably, the solvent in step S4 is butylene glycol.

[0045] Further, the mass fraction of the dried composition in the solvent is 0.5-8%.

[0046] Further, the filter used in step S4 uses a filter membrane of 0.5 μm.

[0047] Further, the present application also provides the use of the composition in cosmetics; the cosmetics include but are not limited to any one of the following: water-based, emulsion-based, cream-based, paste-based, gel-based, and spray-based.

[0048] Further, the cosmetics include but are not limited to any one of the following: facial cleanser, shampoo, hair conditioner, body wash, hand sanitizer, skin toner, skin lotion, skin serum, skin cream, and facial mask.

[0049] Further, the added amount of the efficacy component of the composition in the cosmetics is 0.5-5 wt%, preferably 1-3 wt%.

[0050] Advantageous effects

[0051] 1. The composition of the present application utilizes the synergistic effect of tuberose, peony, and cactus, increases the richness of active efficacy components in the composition, and makes the prepared composition exhibit excellent moisturizing, antioxidant, antibacterial, and bactericidal efficacy on the skin, and a small amount of addition can also achieve excellent anti-inflammatory repair effect, and can be used in cosmetics (such as washing and caring products, skin care products, etc.);

[0052] 2、The mass ratio of tuberose, peony and cactus in the application is (1-4):(0.1-0.4):(1-4), so that the active ingredients in the composition show the best synergistic effect and stability, promote the penetration of each other to the skin, stimulate the skin cells and tissues from the deep, and cure the symptoms of skin allergy;

[0053] 3、The composition of the application has simple raw material composition, but the effect is obvious, and the natural raw materials will not cause adverse stimulation to the skin, and is safe to use. BRIEF DESCRIPTION OF DRAWINGS

[0054] Figure 1 Effect of the composition of Example 1 on the content of IL-6 of H2O2-induced RAW264.7 cells;

[0055] Figure 2 Effect of the composition of Example 1 on the content of IL-1β of H2O2-induced RAW264.7 cells;

[0056] Figure 3 Effect of the composition of Example 1 on the content of TNF-α of H2O2-induced RAW264.7 cells;

[0057] Figure 4 Change of skin water content after using the emulsion containing the composition of Example 1;

[0058] Figure 5 Improvement of skin hematin content after using the emulsion containing the composition of Example 1;

[0059] Figure 6 Improvement of skin trans-epidermal water loss rate (TEWL) after using the emulsion containing the composition of Example 1. DETAILED DESCRIPTION

[0060] EMBODIMENT

[0061] Example 1

[0062] The embodiment provides a composition with barrier repair and soothing effect, and a preparation method thereof, which comprises the following steps:

[0063] S1, vacuum dry tuberose flowers, peony flowers and cactus flowers at 50°C, crush and pass through a 60-mesh sieve, and then take the crushed tuberose flowers, peony flowers and cactus flowers according to a mass ratio of 1:0.2:1 and mix them;

[0064] S2, add 60% ethanol solution (v / v) to the mixture, and the mass ratio of the mixture powder to the ethanol solution is 1:20;

[0065] Afterwards, the mixture is co-extracted by microwave and ultrasonic wave: microwave (power: 900 W) treatment for 10 min, then ultrasonic wave (power: 500 W) treatment for 30 min; the extract is collected;

[0066] S3, the extract is cooled to room temperature, filtered by 150 mesh gauze to obtain a primary filtrate, then centrifuged at 4000 rpm for 20 min, the filtrate is collected, concentrated under reduced pressure and dried to obtain a dry composition;

[0067] S4, the dry composition is re-dissolved in a butanediol solution (60%, v / v) to make the mass concentration of the composition 2%, then filtered by a 0.5 μm microfiltration membrane after mixing.

[0068] Example 2

[0069] The embodiment provides a composition with barrier repair and soothing effects, and a preparation method thereof, which comprises the following steps:

[0070] S1, tuberose flowers, peony flowers and epiphyllum flowers are vacuum dried at 55°C, crushed and sieved through a 60 mesh sieve, and the crushed tuberose flowers, peony flowers and epiphyllum flowers are mixed according to a mass ratio of 1:0.1:4;

[0071] S2, 60% ethanol solution (v / v) is added to the mixture, and the mass ratio of the mixture powder to the ethanol solution is 1:25;

[0072] Afterwards, the mixture is co-extracted by microwave and ultrasonic wave: microwave (power: 900 W) treatment for 10 min, then ultrasonic wave (power: 500 W) treatment for 30 min; the extract is collected;

[0073] S3, the extract is cooled to room temperature, filtered by 150 mesh gauze to obtain a primary filtrate, then centrifuged at 4000 rpm for 20 min, the filtrate is collected, concentrated under reduced pressure and dried to obtain a dry composition;

[0074] S4, the dry composition is re-dissolved in a butanediol solution (60%, v / v) to make the mass concentration of the composition 2%, then filtered by a 0.5 μm microfiltration membrane after mixing.

[0075] Example 3

[0076] The embodiment provides a composition with barrier repair and soothing effects, and a preparation method thereof, which comprises the following steps:

[0077] S1, the tuberose flower, peony flower, cactus flower is dried under vacuum at 35 °C, after crushing, pass through 60 mesh sieve, according to 5:0.5:1 mass ratio of the crushed tuberose flower, peony flower and cactus flower is mixed;

[0078] S2, 60% ethanol solution (v / v) is added to the mixture, and the mass ratio of the mixture powder to the ethanol solution is 1:15;

[0079] Then the mixture is extracted by microwave and ultrasonic wave: microwave (power: 1200W) treatment for 5min, then ultrasonic wave (power: 800W) treatment for 15min; collect the extract;

[0080] S3, the extract is cooled to room temperature, and the initial filtrate is obtained by filtering with 200 mesh gauze, and then centrifuged at 2000 rpm for 30 min, the filtrate is collected, concentrated under reduced pressure and dried to obtain a dry composition;

[0081] S4, the dry composition is re-dissolved in butanediol solution (60%, v / v) to obtain a composition with a mass concentration of 1%, and then filtered with a 0.5 μm microfiltration membrane.

[0082] Example 4

[0083] The embodiment 1 is basically the same, the difference is that the mass ratio of the crushed tuberose flower, peony flower and cactus flower is 2:0.2:1.

[0084] Example 5

[0085] The embodiment 1 is basically the same, the difference is that the mass ratio of the crushed tuberose flower, peony flower and cactus flower is 3:0.2:1.

[0086] Example 6

[0087] The embodiment 1 is basically the same, the difference is that the mass ratio of the crushed tuberose flower, peony flower and cactus flower is 4:0.2:1.

[0088] Example 7

[0089] The embodiment 1 is basically the same, the difference is that the mass ratio of the crushed tuberose flower, peony flower and cactus flower is 1:0.1:1.

[0090] Example 8

[0091] The embodiment 1 is basically the same, the difference is that the mass ratio of the crushed tuberose flower, peony flower and cactus flower is 1:0.3:1.

[0092] Example 9

[0093] The example 1 is basically identical, except that the mass ratio of the pulverized tuberose flower, peony flower and epiphyllum flower is 1 : 0.4 : 1.

[0094] Example 10

[0095] The example 1 is basically identical, except that the mass ratio of the pulverized tuberose flower, peony flower and epiphyllum flower is 1 : 0.2 : 2.

[0096] Example 11

[0097] The example 1 is basically identical, except that the mass ratio of the pulverized tuberose flower, peony flower and epiphyllum flower is 1 : 0.2 : 3.

[0098] Example 12

[0099] The example 1 is basically identical, except that the mass ratio of the pulverized tuberose flower, peony flower and epiphyllum flower is 1 : 0.2 : 4.

[0100] Comparative Example 1

[0101] The example 1 is basically identical, except that the mass ratio of the tuberose flower, peony flower and epiphyllum flower is 1 : 0 : 0.

[0102] Comparative Example 2

[0103] The example 1 is basically identical, except that the mass ratio of the tuberose flower, peony flower and epiphyllum flower is 0 : 1 : 0.

[0104] Comparative Example 3

[0105] The example 1 is basically identical, except that the mass ratio of the tuberose flower, peony flower and epiphyllum flower is 0 : 0 : 1.

[0106] Comparative Example 4

[0107] The example 1 is basically identical, except that the mass ratio of the tuberose flower, peony flower and epiphyllum flower is 1 : 0.2 : 0.

[0108] Comparative Example 5

[0109] The example 1 is basically identical, except that the mass ratio of the tuberose flower, peony flower and epiphyllum flower is 1 : 0.2 : 6.

[0110] Comparative Example 6

[0111] The example 1 is basically identical, except that the mixed solution is extracted by microwave and ultrasonic wave: first microwave (power: 900W) treatment for 10min, then ultrasonic wave (power: 1000W) treatment for 30min; the extraction liquid is collected.

[0112] Performance test method:

[0113] 1. Stability test: The composition of the examples is placed at -4°C, 25°C, 40°C for continuous storage for 6 months, and observed every 7 days to see if there are any adverse phenomena such as turbidity, discoloration, flocculation, delamination, precipitation, and to judge the storage stability thereof;

[0114] 2. In vitro antioxidant test:

[0115] The antioxidant capacity of the composition is determined by spectrophotometry through DPPH free radical scavenging capacity determination. DPPH, also known as 1,1-diphenyl-2-trinitrobenzene hydrazine, is a very stable free radical with a nitrogen center. Its anhydrous ethanol solution is purple. When a free radical scavenger is added, the number of free radicals decreases, the absorbance decreases, and the solution color becomes lighter, which can evaluate the ability to scavenge free radicals.

[0116] 40 μL of each composition (containing 2% active ingredient) is measured with a pipette, 160 μL of DPPH ethanol solution (200 μg / ml) is added for dilution and mixing, and after mixing, it is reacted at room temperature in the dark for 30 min, and the absorbance A is measured at 517 nm. Three sets of parallel experiments are set for each group to calculate the average value, and the calculation method is as follows:

[0117] Scavenging rate (%) = [1-(A1-A2) / A0] x 100

[0118] In the formula: A0 is the absorbance of the DPPH solution without adding each composition; A1 is the absorbance of the DPPH solution after adding each composition and reacting; A2 is the absorbance of the DPPH solution after adding each composition and mixing.

[0119] 3. Anti-inflammatory effect test:

[0120] Inflammation is a series of complex interactions between soluble factors and cells that may occur after tissue responds to trauma, infection, ischemia, toxicity, or immune damage. According to literature reports, cytokines produced during the inflammatory response, such as tumor necrosis factor-α (TNF-α), nitric oxide (NO), and interleukin-6 (IL-6), interleukin-1β (IL-1β) play a very important role in immune-inflammatory response.

[0121] The sample prepared in Example 1 was formulated into a solution with a mass fraction of 0.005%, 0.05%, and 0.1%. The experiment was divided into a blank control group, an H2O2 stimulation group (model group), and a drug group. HaCaT cells growing to the logarithmic growth phase in the culture dish were recovered into a centrifuge tube in a sterile operation table, counted by a cell counter, inoculated into a 24-well cell culture plate at 4.0 x 104 cells / well, and cultured for 24 h. The blank control group was DMEM medium, 100 μmol / L of H2O2 was cultured for 6 h, and then the sample was added. It was placed in a 37°C incubator for 24 h, the cell supernatant was collected, and the TNF-α, IL-1β, and IL-6 content levels in the supernatant were detected according to the kit operation;

[0122] 4. Efficacy experiment:

[0123] Sixty sensitive skin volunteers were screened and divided into two groups, with 30 people in each group, who used the emulsion containing the composition of Example 1 (containing 2 wt% of the active ingredients of the composition) and the blank emulsion, respectively, once a day in the morning and evening, for 28 days. The skin water content was measured by Corneometer, the trans-epidermal water loss rate TEWL was measured by Tewameter, and the red blood vessels were photographed and measured by Antera 3D. The test results are shown in Figure 4 , Figure 5 , Figure 6 .

[0124] Performance test results:

[0125] The test results are shown in Table 1.

[0126] Table 1

[0127]

[0128]

Claims

1. A composition having barrier repair, soothing efficacy, characterized in that, The raw materials of the composition include tuberose flower, peony flower and cactus flower, and the mass ratio of the tuberose flower, the peony flower and the cactus flower is (1-4):(0.1-0.4):(1-4). The preparation method of the composition comprises the following steps: S1, crushing, drying and sieving the raw materials of the composition, and then mixing them according to the corresponding mass ratio to obtain mixed raw materials; S2, adding 60% ethanol solution into the mixed raw materials, and performing microwave treatment and ultrasonic treatment for co-extraction, and collecting the extraction liquid; S3, filtering, concentrating and drying the extraction liquid to obtain a dried composition; S4, re-dissolving the dried composition in 60% butanediol solution, and then filtering to obtain the composition; In the step S2, the microwave treatment and the ultrasonic treatment are performed in sequence or alternately, the ultrasonic power is 300-800W, and the ultrasonic time is 15-60min.

2. The composition of claim 1, wherein, In the step S2, the microwave power is 700-1200W, and the microwave time is 5-20min.

3. The composition according to any one of claims 1-2 for use in cosmetics.

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