Preparation method and application of composition containing prickle oil

Abstract

The invention relates to the technical field of cosmetics, in particular to a preparation method and application of a composition containing prickle fruit oil. The composition comprises the following raw materials by weight: prickle fruit oil, ceramide E, triple collagen, citrus extract, bisabolol and panthenol. The invention combines prickle fruit oil with other skin care ingredients such as citrus fruit extract to synergistically improve the moisturizing, soothing and other effects of the composition.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, and in particular to a preparation method and application of a composition containing prickle fruit oil. Background Art

[0002] Essence is a common skin care product for the face, which usually has the effects of anti-aging, anti-wrinkle, moisturizing, whitening, freckle removal, etc. Some plant extracts are often added to the existing essence to enhance the effect of the essence in repairing the skin.

[0003] Prinsepia utilis Royle belongs to the genus Prinsepia of the Rosaceae family and is the fruit of Prinsepia utilis Royle. Prinsepia utilis Royle is widely distributed in Yunnan, Guizhou, Sichuan and Tibet in my country. Its kernel contains a variety of microorganisms, trace elements, amino acids and fatty acids. Its structure is very close to the lipid ratio of the human body, has high permeability and is easily absorbed by the skin. Based on the above characteristics, Prinsepia utilis Royle oil has the active effects of maintaining skin moisture and elasticity, improving the degeneration of the skin's stratum corneum, and repairing rough skin.

[0004] In the prior art, many people have used prickle fruit oil or its fermented extract to prepare skin care products with various functions. However, the inventors have found that the effects of existing skin care products containing prickle fruit oil in terms of moisturizing and skin repairing still need to be improved. Summary of the invention

[0005] The purpose of the present invention is to provide a preparation method and application of a composition containing prickle fruit oil. In the present invention, a specific preparation method is selected to extract prickle fruit kernels, and the obtained prickle fruit oil has a high content of effective skin care components. The prickle fruit oil is combined with other skin care components such as triple collagen to jointly improve the moisturizing, anti-wrinkle, whitening and freckle removal effects of the composition of the present invention.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] The present invention provides a composition containing prickle oil, which comprises the following raw materials in parts by weight:

[0008] 0.1-0.5 parts of prickle fruit oil, 0.1-0.5 parts of ceramide E, 0.01-0.3 parts of triple collagen, 0.1-2 parts of citrus fruit extract, 0.01-0.3 parts of bisabolol, and 0.1-2 parts of panthenol. The preparation method of prickle fruit oil is as follows:

[0009] S1. The crushed fruit is extracted and distilled to obtain prickle oil;

[0010] S2. The prickle oil obtained in S1 is mixed with ceramide E, triple collagen, citrus fruit extract, bisabolol and panthenol to obtain a composition.

[0011] In one or more embodiments, the extraction method in step S1 is supercritical carbon dioxide extraction, and the extraction conditions are: temperature 30°C-42°C, pressure 11.00MPa-14.00MPa, time 120min-150min, and carbon dioxide flow rate 25kg / h-35kg / h.

[0012] In one or more embodiments, the distillation method in step S1 is two-stage molecular distillation, and the distillation conditions are: feed temperature is 40°C-45°C, injection rate is 480-530mL / h, first stage evaporation temperature is 110°C-115°C, and second stage evaporation temperature is 60°C-65°C.

[0013] Furthermore, the present invention also provides a method for preparing triple collagen in the composition, which comprises the following raw materials by weight:

[0014] Triple collagen includes recombinant type 1 collagen, type 3 collagen, and type 17 collagen, and the mass ratio thereof is 1:0.5-2:1-1.5.

[0015] S1: weigh and mix recombinant type 1 collagen, type 3 collagen, type 17 collagen, 1,3-propylene glycol and purified water, and filter to obtain triple collagen.

[0016] Finally, the present invention also provides an essence comprising the above-mentioned prickle fruit oil composition, which comprises the following raw materials by weight percentage:

[0017] The composition is 5%-8%, glycerol is 20%-40%, and sterile deionized water is used to make up the difference. The preparation method is as follows:

[0018] Add 20%-40% glycerol into a beaker, evenly disperse 5%-8% of the composition in the glycerol, and then add sterile deionized water and stir evenly.

[0019] In the essence of the present invention, the conditions and processes selected in the present invention are used in combination to extract the thorny tree kernels to obtain more fatty acids, amino acids, polypeptides, trace elements and other substances. When the preparation process conditions are changed to extract the thorny tree kernels, the obtained fatty acids, amino acids, polypeptides, trace elements and other skin care ingredients are less, so the effects of moisturizing and repairing the skin are poor.

[0020] In the essence of the present invention, the prickle fruit oil has the effects of moisturizing the skin and regulating the balance of sebum secretion, and can also promote epidermal cell regeneration and solve the problem of skin infection. Secondly, the prickle fruit oil has a bactericidal function, and can relieve and treat acne, mild skin infection and skin inflammation. And the prickle fruit oil has a good inhibitory effect on bacteria and fungi, and can especially inhibit the growth of mold. The prepared prickle fruit oil is added to the essence of the present invention, and the prickle fruit oil can not only play an antibacterial effect, so that the essence can be stored for a long time without adding a common cosmetic preservative, but also the prickle fruit oil can work together with the other components to enhance the moisturizing and water-locking functions of the essence, reduce wrinkles on the skin surface, and can also relieve inflammation and capillary dilation on the skin surface.

[0021] The essence of the present invention adds triple collagen, which has the functions of enhancing skin elasticity, whitening and lightening spots, delaying aging, repairing skin barrier, maintaining moisture, moisturizing and softening. The addition of triple collagen makes the essence have the functions of protecting tissue, hydrophilic, absorbing water and preventing moisture from radiating; triple collagen can also promote tissue growth, cell metabolism, and soften stratum corneum protein. The essence of the present invention adds ceramide E, which has the function of resisting skin aging, can reduce and prevent the problem of dull and yellow skin during aging; it can also repair the stratum corneum and play the role of locking water and moisturizing. The amount of ceramide E in the present invention is appropriate, which can play the above good role; but the excessive amount of ceramide E will play the opposite role. The essence of the present invention adds panthenol, bisabolol, and citrus fruit extract, all of which have antioxidant properties and can prevent skin oxidation and aging by resisting free radicals in the human body. In cosmetics, carnosine can whiten the skin and prevent skin aging. It can maintain uniform skin tone, enhance the skin's own resistance and self-healing ability, purify the skin, maintain normal internal circulation of the skin, accelerate the excretion of skin metabolic products, maintain normal skin metabolism, and prevent skin aging.

[0022] The ceramide E, triple collagen, citrus fruit extract, bisabolol and panthenol in the present invention work together and are combined with the prickle oil, so that the essence of the present invention has a good skin repair effect, brightens the skin and reduces texture. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 Demonstration of the effects of some volunteers.

[0024] Figure 2 Demonstration of the effects of some volunteers.

[0025] Figure 3 Hyaluronidase inhibition rate of Examples 7 to 13 and Comparative Examples 10 to 22.

[0026] Figure 4Skin redness test of Examples 14 to 16 and Comparative Examples 23 to 24. DETAILED DESCRIPTION

[0027] In order to better illustrate the purpose, technical scheme and advantages of the present invention, the present invention is further described by the following examples. It should be understood that the examples of the present invention are only used to illustrate the technical effects of the present invention, but not to limit the scope of protection of the present invention. In the examples, the methods used are conventional methods unless otherwise specified.

[0028] Some of the raw materials in the present invention come from the following sources:

[0029] Prinsepia utilis Royle kernel: purchased from Xi'an Qiancao Biotechnology Co., Ltd.

[0030] Ceramide E: Suzhou Yaxu Fine Chemicals Co., Ltd.

[0031] Citrus fruit (Citrus Reticulata) extract: purchased from Xi'an Qiancao Biotechnology Co., Ltd.

[0032] Bisabolol: Suzhou Yaxu Fine Chemicals Co., Ltd.

[0033] Panthenol: Suzhou Yaxu Fine Chemicals Co., Ltd.

[0034] Recombinant type 1 collagen: purchased from Nanjing Peptide Valley Biotechnology Co., Ltd.

[0035] Type 3 collagen: purchased from Nanjing Peptide Valley Biotechnology Co., Ltd.

[0036] Type 17 collagen: purchased from Nanjing Peptide Valley Biotechnology Co., Ltd.

[0037] Vegetable oil: purchased from Walmart supermarket, Luhua 5S pressed peanut oil.

[0038] The preparation method of prickle oil of the present invention is as follows:

[0039] S1. Take the thorn nut kernels and pre-dry them at 55±5°C for 10 hours, then use supercritical extraction and distillation to obtain thorn nut oil; the specific parameters are shown in Table 1.

[0040] S2. The prickle oil obtained in S1 is mixed with ceramide E, triple collagen, citrus fruit extract, bisabolol and panthenol to obtain a composition.

[0041] Table 1 Preparation process parameters of prickle oil

[0042]

[0043] The mass ratios of recombinant type 1 collagen, type 3 collagen, and type 17 collagen in the triple collagen described in S2 are shown in Table 2.

[0044] Table 2 Preparation parameters of triple collagen

[0045]

[0046]

[0047] In S2, the prickle oil obtained in S1 is mixed with ceramide E, triple collagen, citrus fruit extract, bisabolol and panthenol to obtain a composition.

[0048] The specific parameters of the composition are shown in Table 3.

[0049] Table 3 Composition preparation parameters

[0050]

[0051]

[0052] Essence preparation parameters, the rest was made up with sterile deionized water. See Table 4 for details.

[0053] Table 4 Essence preparation parameters

[0054] Experimental Group Composition glycerin Composition No. Embodiment 14 5％ 20％ Example 8 Embodiment 15 7％ 30％ Example 8 Example 16 9％ 40％ Example 8 Comparative Example 24 15％ 30％ Example 8 Comparative Example 25 0 30％ Example 8

[0055] 1. Safety Testing

[0056] With reference to the 2015 Technical Specifications for Safety of Cosmetics, the compositions of Examples 7 to 16 were evaluated for cosmetic irritation. The test method was a skin patch test, and 100 randomly distributed people aged 16 to 65 were tested.

[0057] Test method: Put the test substance into the patch tester, the dosage is 0.020-0.025g, cover the patch tester with the test substance on the back or forearm of the subject with non-irritating cloth-based tape, and gently press it with the palm to make it evenly adhere to the skin surface for 24 hours. After removing the test patch tester, observe the skin reaction after the indentation disappears 30 minutes later. If the result is negative, observe again 24 hours and 48 hours after the patch test.

[0058] Evaluation criteria:

[0059] Grade 0: negative reaction;

[0060] Grade 1: Suspicious reaction, only slight erythema;

[0061] Grade 2: weak positive reaction, erythema, infiltration, edema, and papules may be present;

[0062] Grade 3: Strong positive reaction, erythema, infiltration, edema, papules, and the reaction may extend beyond the test area;

[0063] Grade 4: Very strong positive reaction, obvious erythema, severe infiltration, edema, confluent herpes, and the reaction exceeds the test area.

[0064] Test results: Skin reactions were negative in all subjects.

[0065] 2. In vitro hyaluronidase inhibition experiment verifies moisturizing and soothing

[0066] Hyaluronic acid, also known as hyaluronic acid, is a powerful moisturizer. It can attract and lock in moisture, maintain skin moisture, and improve dry, rough and dehydrated skin conditions. Hyaluronic acid can also enhance the biological activity of cells and has a strong synergistic effect on repairing and protecting the skin. Therefore, by inhibiting hyaluronidase, the decomposition rate of hyaluronic acid in the skin is reduced, thereby achieving long-term moisture retention and repair of the skin, which is of great significance.

[0067] (1) Take four groups of clean test tubes, label them A, B, C, and D, add 50 μL of the sample (10 mg / L) to test tubes A and B, add 50 μL of distilled water to test tubes C and D, add 50 μL of 500 U / mL hyaluronidase solution to test tubes A and C, add 50 μL of pH 5.6 acetate buffer to test tubes B and D, and place the test tubes in a 37°C incubator for 20 min.

[0068] (2) Add 10 μL of 2.5 mol / L CaCl2 solution to each of the four test tubes and place them in an incubator at 37°C for 20 min.

[0069] (3) Add 50 μL of 0.5 mg / mL sodium hyaluronate solution to test tubes A and C, and add 50 μL of pH 5.6 acetate buffer to test tubes B and D. Place the test tubes in a 37°C incubator for 40 min and then take them out and place them at room temperature for 10 min.

[0070] (4) Add 50 μL of distilled water, 10 μL of 5 mol / L NaOH solution, and 50 μL of acetylacetone solution to the four test tubes respectively. Place the test tubes in a boiling water bath for 30 min, then in an ice bath for 10 min, and finally at room temperature for 10 min.

[0071] (5) Add 100 μL of P-DAB reagent to each test tube, and then measure the absorbance at 530 nm using an ELISA reader.

[0072]

[0073] Wherein: A is the OD value of the sample solution; B is the OD value of the sample blank; C is the OD value of the control sample solution; D is the OD value of the control sample blank.

[0074] Table 5 Hyaluronidase inhibition rate test results

[0075]

[0076]

[0077] From the results analysis of Table 5: From the results of Examples 7-13 and Comparative Examples 10-23, it can be seen that the composition provided by the embodiments of the present invention has good hyaluronidase inhibition effect, especially the effect of Example 8 is better, indicating that using Example 2 as the preparation process parameters of the clava utilis oil and using Example 5 as the preparation parameters of the triple collagen can effectively improve the inhibitory effect of the composition on hyaluronidase.

[0078] From the results of Example 8 and Comparative Examples 10-15 and 23, it is shown that the components defined in the present invention have a certain synergistic effect, and the effect achieved by the present invention cannot be achieved by omitting or replacing any one of them.

[0079] Comparing Example 8 with Comparative Example 16, Example 8 has the best effect, indicating that within the mass range of the components provided by the present invention, it has a significant effect of inhibiting hyaluronidase, and excessive addition cannot significantly improve its hyaluronic acid inhibition effect.

[0080] Comparative Example 8 and Comparative Examples 17-19 show that the use of the extraction process defined in the present invention can enable the present invention to achieve maximum efficacy in inhibiting hyaluronic acid, and changing any extraction process not defined in the present invention cannot achieve the effect achieved by the present invention.

[0081] By comparing the results of Example 8 with Comparative Examples 20-22, it can be seen that the use of the triple collagen preparation ratio specified in the present invention can enable the present invention to achieve the maximum efficacy in inhibiting hyaluronic acid, and changing any preparation ratio not specified in the present invention cannot achieve the effect achieved by the present invention.

[0082] 3. Human body 30s instant soothing effect test

[0083] Volunteer selection criteria: a) healthy volunteers aged 18 to 60 years old; b) subjects with no skin lesions / dermatitis or other factors that affect the measurement at the test site; c) those with sensitive and dry faces; d) those with positive lactic acid stinging test.

[0084] The number of volunteers is guaranteed to be more than 30, and the subjects should mainly engage in indoor activities to avoid long-term exposure to light and high temperature or cold environments.

[0085] 33 volunteers aged 22-55 with sensitive facial skin (lactic acid stinging test score ≥ 3 points on both sides of the nose) and dry East Asian skin were selected. A sample group and a blank group (i.e., a model control group: no sample was applied) were set up, and the test areas were randomly distributed on the left and right faces of the volunteers. After using the essence of the present invention for 30 seconds, the red image was collected by the skin facial image analyzer VISIA 7, and the skin redness a* value was analyzed by Image-Pro Plus (IPP) analysis software to evaluate its moisturizing and soothing effects. The laboratory temperature was 21±1°C; the relative humidity was 50%±10%.

[0086] The experiment adopted the self-before and after control method and the blank group control method. The test cycle was 30s, and the skin image collection times were 2 times, 0s and 30s respectively. Set up a sample group and a blank group (i.e., model control group: no sample was applied), and the test areas were randomly distributed on the left and right faces of the volunteers. Apply the sample at a dosage of 0.25g / half face. Select qualified subjects according to the requirements, and when the volunteers visit, sign the volunteer informed consent form with them, collect skin images and questionnaire data after using the product for 30s. When visiting, after washing the face with the designated cleanser, sit quietly in the test environment for about 20-30 minutes. After the sitting is over, start the test.

[0087] With the help of facial image analyzer VISIA 7 ( Company, USA), the instrument uses 3 different light sources to capture facial images and can intuitively observe changes in facial skin conditions.

[0088] The red image a* value was analyzed using Image-Pro Plus software.

[0089] Skin redness a* value: The a* value represents the redness of the skin. An increase in the a* value indicates an increase in the vascular reactivity of the skin.

[0090] The data were analyzed using SPSS19.0 data analysis software.

[0091] Table 6 Skin redness a* experimental results

[0092]

[0093]

[0094] From the analysis of the results in Table 6: Comparing Examples 14-16 and Comparative Examples 24-25, the results show that the skin redness a* of Examples 14-16 is reduced, and the reduction degree is the highest in Example 15, indicating that the essence prepared using the composition of the present invention has a soothing effect on the skin, which does not show a linear trend with the amount of addition.

[0095] Comparative Example 15 and Comparative Example 24 show that excessively high addition of the composition cannot significantly reduce the average skin redness value, and the addition amount defined in the present invention can maximize the reduction of the skin redness a* value by the essence.

[0096] Comparative Example 15, Comparative Example 25 and Blank indicate that the use of the composition prepared by the present invention can achieve the maximum effect of the essence on reducing the a* value of skin redness.

[0097] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A composition containing utilitarian fruit oil, characterized in that: include: 0.1-0.5 parts of prickle oil; Ceramide E 0.1-0.5 parts; Triple collagen 0.01-0.3 parts; 0.1-2 parts of citrus fruit extract; Bisabolol 0.01-0.3 parts; 0.1-2 parts of panthenol; The citrus fruit extract was purchased from Xi'an Qiancao Biotechnology Co., Ltd. The composition preparation method comprises the following steps: S1. crushing, extracting and distilling the prickle fruit to obtain prickle fruit oil; S2. The prickle oil obtained in S1 is mixed with ceramide E, triple collagen, citrus fruit extract, bisabolol, and panthenol to obtain a composition; The green thorn fruit is made of green thorn nut kernel; The extraction method described in S1 is supercritical carbon dioxide extraction, and the extraction conditions are: temperature 30°C-42°C, pressure 11.00MPa-14.00MPa, time 120min-150min, and carbon dioxide flow rate 25kg / h-35kg / h; The distillation method described in S1 is a two-stage molecular distillation method, and the distillation conditions are: the feed temperature is 40°C-45°C, the injection rate is 480-530mL / h, the first evaporation temperature is 110°C-115°C, and the second evaporation temperature is 60°C-65°C; The triple collagen preparation method comprises the following steps: S1. Weigh and mix recombinant type 1 collagen, type 3 collagen, type 17 collagen, 1,3-propylene glycol and purified water, and filter to obtain triple collagen.

2. The composition according to claim 1, characterized in that include: 0.25 parts of prickle oil; Ceramide E 0.3 parts; Triple collagen 0.1 part; 0.15 parts of citrus fruit extract; 0.15 parts of bisabolol; 1 part panthenol.

3. The composition according to claim 1, characterized in that Triple collagen includes recombinant type 1 collagen, type 3 collagen, and type 17 collagen, and the mass ratio thereof is 1:0.5-2:1-1.

5.

4. Use of the composition according to any one of claims 1 to 3 in the preparation of cosmetics.

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