Preparation method and application of fermentation compound for improving sleep

Abstract

The present invention relates to the technical field of biological products, and discloses a preparation method and application of a fermentation complex for improving sleep, including 40-75 parts by weight of scutellaria, 30-70 parts by weight of white peony root, 6-15 parts by weight of schisandra chinensis, 40-70 parts by weight of astragalus, 30-80 parts by weight of sophora flavescens, 25-40 parts by weight of liquorice, 0.3-1.8 parts by weight of julibrissin bark extract, 0.5-2 parts by weight of Rehmannia glutinosa extract, and 1-4 parts by weight of lactococcus lactis fermentation broth; the present invention optimizes liquid seed culture medium and fermentation culture medium to obtain a lactococcus lactis fermentation broth with a high γ-aminobutyric acid content. The obtained fermentation complex can effectively improve sleep and treat insomnia.

Description

Technical Field

[0001] The invention relates to the technical field of biological products, and in particular to a preparation method and application of a fermentation complex for improving sleep. Background Art

[0002] Long-term use of sleeping pills and other chemical drugs by insomnia patients will cause damage to the liver, kidney function, respiratory system, etc. Taking Chinese herbal drinks such as Anshen Decoction can effectively improve sleep problems and relieve insomnia symptoms.

[0003] Albizzia julibrissin bark is the dried bark of Albizzia julibrissin, a common tranquilizer containing a large amount of triterpenes, flavonoids, alkaloids, polysaccharides and other nutrients. Effective extraction of nutrients in Albizzia julibrissin bark has important applications. Lactococcus lactis can produce lactic acid, soy isoflavones, γ-aminobutyric acid and other nutrients after fermentation. Among them, γ-aminobutyric acid can relieve anxiety, improve sleep quality and other effects. The present invention aims to provide a fermentation complex containing Albizzia julibrissin bark extract, Rehmannia glutinosa extract and Lactococcus lactis fermentation broth for improving sleep and treating insomnia. Summary of the invention

[0004] The technical problem solved by the present invention is to provide a fermentation complex containing Lactococcus lactis fermentation liquid, which can be used for improving sleep and treating insomnia.

[0005] The present invention provides a technical solution: a fermentation complex for improving sleep, the fermentation complex comprising 40-75 parts by weight of scutellaria baicalensis, 30-70 parts by weight of white peony root, 6-15 parts by weight of schisandra chinensis, 40-70 parts by weight of astragalus, 30-80 parts by weight of sophora flavescens, 25-40 parts by weight of liquorice, 0.3-1.8 parts by weight of julibrissin bark extract, 0.5-2 parts by weight of rehmannia glutinosa extract, and 1-4 parts by weight of lactococcus lactis fermentation liquid.

[0006] Furthermore, a method for preparing a fermentation complex for improving sleep is as follows: Scutellaria baicalensis, white peony root, Schisandra chinensis, Astragalus membranaceus, Sophora flavescens, and Licorice are cleaned, added to distilled water, boiled and then simmered over low heat, cooled, and Albizzia julibrissin extract, Rehmannia glutinosa extract, and Lactococcus lactis fermentation liquid are added, mixed, and a fermentation complex is obtained.

[0007] Furthermore, the preparation method of the Albizzia julibrissin bark extract is: clean the Albizzia julibrissin bark, dry it, grind and sieve it, then place the Albizzia julibrissin bark fine powder in an ethanol solvent, and control the ratio of the Albizzia julibrissin bark fine powder to ethanol to be (10-60) g:1L; extract in a microwave reactor for 5-10 minutes, control the power of the microwave reactor to be 400-800W, filter, wash the precipitate with ethanol, combine the filtrate, and dry it to obtain the Albizzia julibrissin bark extract.

[0008] Furthermore, the preparation method of Rehmannia glutinosa extract is as follows: clean Rehmannia glutinosa, dry, grind and sieve, then place Rehmannia glutinosa fine powder in ethanol solvent and distilled water solvent, control the ratio of Rehmannia glutinosa fine powder, ethanol solvent and distilled water solvent to be (20-100) g: (0.4-0.8) L: 1 L, extract in a microwave reactor for 10-20 min, control the power of the microwave reactor to be 400-800 W; filter, wash the precipitate with ethanol, combine the filtrate, dry and obtain the Rehmannia glutinosa extract.

[0009] Further, the preparation method of Lactococcus lactis fermentation broth is:

[0010] (1) Add liquid seed culture medium to a flask, use an inoculation loop to take 1-2 rings of Lactococcus lactis and inoculate them into the liquid seed culture medium, and culture at 30° C. for 12-18 hours to obtain a culture solution.

[0011] (2) adding fermentation medium to the flask, transferring the culture solution to the flask, capping the flask, culturing at 30° C. for 18-24 h, centrifuging the culture, and then heating and boiling the centrifuge solution for 5-10 min to obtain a Lactococcus lactis fermentation solution.

[0012] Furthermore, the liquid seed culture medium includes 100 parts by weight of distilled water, 0.8-1 parts by weight of peptone, 0.15-0.2 parts by weight of casein peptide, 0.8-1.2 parts by weight of beef extract, 0.4-0.5 parts by weight of yeast extract powder, 1.5-2.5 parts by weight of glucose, 0.5-0.6 parts by weight of sodium acetate, 0.016-0.018 parts by weight of dipotassium hydrogen phosphate, 0.04-0.05 parts by weight of magnesium sulfate, 0.02-0.025 parts by weight of manganese sulfate, 0.15-0.2 parts by weight of diamine citrate, and 0.1-0.13g of Tween 80.

[0013] Furthermore, the fermentation medium includes 100 parts by weight of distilled water, 0.8-1 parts by weight of yeast extract powder, 0.05-0.08 parts by weight of casein peptide, 0.8-1.4 parts by weight of glucose, 0.1-0.14 parts by weight of dipotassium hydrogen phosphate, 0.07-0.08 parts by weight of magnesium sulfate, 0.01-0.012 parts by weight of sodium chloride, and 0.6-0.8 parts by weight of sodium glutamate.

[0014] The technical effect of the present invention is as follows: the present invention optimizes the liquid seed culture medium and the fermentation culture medium to obtain a lactococcus lactis fermentation liquid with a high content of gamma-aminobutyric acid. The present invention adopts a microwave extraction method to effectively extract polysaccharides, flavonoids and other substances in the bark of Albizzia julibrissin and Rehmannia glutinosa to obtain the bark of Albizzia julibrissin extract and the Rehmannia glutinosa extract.

[0015] The invention scientifically compounds scutellaria baicalensis, white peony root, schisandra chinensis, astragalus, sophora flavescens and liquorice, and simultaneously adds lactococcus lactis fermentation liquid, julibrissin bark extract and rehmannia glutinosa extract to obtain a fermentation complex, which can improve sleep and achieve a total effective rate of 90-100% in treating insomnia. DETAILED DESCRIPTION

[0016] The following will clearly and completely describe the concept of the present application and the technical effects produced in combination with the embodiments, so as to fully understand the purpose, features and effects of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of them. Based on the embodiments of the present application, other embodiments obtained by those skilled in the art without creative work are all within the scope of protection of the present application.

[0017] Example 1

[0018] (1) Add 0.8 g of peptone, 0.2 g of casein peptide, 1 g of beef extract, 0.5 g of yeast extract powder, 1.8 g of glucose, 0.5 g of sodium acetate, 0.017 g of dipotassium hydrogen phosphate, 0.04 g of magnesium sulfate, 0.02 g of manganese sulfate, 0.18 g of diammonium citrate, and 0.13 g of Tween 80 to 100 g of distilled water and mix well to obtain a liquid seed culture medium.

[0019] (2) Add 0.8 g yeast extract powder, 0.06 g casein peptide, 1 g glucose, 0.12 g dipotassium hydrogen phosphate, 0.08 g magnesium sulfate, 0.01 g sodium chloride, and 0.8 g sodium glutamate to 100 g distilled water and mix well to obtain a fermentation medium.

[0020] (3) Add liquid seed culture medium to the flask, use an inoculation loop to take 2 loops of Lactococcus lactis and inoculate them into the liquid seed culture medium, and culture at 30° C. for 18 h to obtain a culture solution.

[0021] (4) Add 30 mL of fermentation medium to the flask, transfer 1.5 mL of culture solution to the flask, cover the flask with a stopper, and culture at 30° C. for 24 h. Centrifuge the culture, and then heat and boil the centrifuge for 5 min to obtain a Lactococcus lactis fermentation solution with a high γ-aminobutyric acid content.

[0022] (5) Clean the bark of Albizzia julibrissin, dry it, grind it and sieve it. Then, place 20 g of the fine powder of the bark of Albizzia julibrissin in 500 mL of ethanol solvent and extract it in a microwave reactor for 5 min. The power of the microwave reactor is controlled to 800 W. Filter it, wash the precipitate with ethanol, combine the filtrate, and dry it to obtain the bark of Albizzia julibrissin extract.

[0023] (6) Clean the Radix Rehmanniae glutinosae, dry it, grind it and sieve it, then place 10 g of the Radix Rehmanniae glutinosae fine powder in 200 mL of ethanol solvent and 500 mL of distilled water solvent, extract it in a microwave reactor for 15 minutes, and control the power of the microwave reactor to 400 W; filter, wash the precipitate with ethanol, combine the filtrate, and dry it to obtain the Radix Rehmanniae glutinosae extract.

[0024] (7) Wash 45 g of Scutellaria baicalensis, 40 g of White Peony Root, 6 g of Schisandra chinensis, 60 g of Astragalus, 80 g of Sophora flavescens, and 25 g of Licorice, add them to 2 L of distilled water, boil them, and then simmer for 60 min. After cooling, add 0.3 g of Albizzia julibrissin bark extract, 1.5 g of Rehmannia glutinosa extract, and 2 g of Lactococcus lactis fermentation broth, mix well, and obtain a fermentation complex.

[0025] Example 2

[0026] (1) Add 0.8 g of peptone, 0.18 g of casein peptide, 1.2 g of beef extract, 0.48 g of yeast extract powder, 2 g of glucose, 0.5 g of sodium acetate, 0.016 g of dipotassium hydrogen phosphate, 0.046 g of magnesium sulfate, 0.02 g of manganese sulfate, 0.15 g of diamine citrate, and 0.13 g of Tween 80 to 100 g of distilled water and mix well to obtain a liquid seed culture medium.

[0027] (2) Add 0.8 g yeast extract powder, 0.05 g casein peptide, 0.8 g glucose, 0.12 g dipotassium hydrogen phosphate, 0.08 g magnesium sulfate, 0.01 g sodium chloride, and 0.7 g sodium glutamate to 100 g distilled water and mix well to obtain a fermentation medium.

[0028] (3) Add liquid seed culture medium to the flask, use an inoculation loop to take one loop of Lactococcus lactis and inoculate it into the liquid seed culture medium, and culture at 30° C. for 12 h to obtain a culture solution.

[0029] (4) Add 30 mL of fermentation medium to the flask, transfer 1.5 mL of culture solution to the flask, cover the flask with a stopper, and culture at 30° C. for 24 h. Centrifuge the culture, and then heat and boil the centrifuge for 5 min to obtain a Lactococcus lactis fermentation solution with a high γ-aminobutyric acid content.

[0030] (5) Clean the bark of Albizzia julibrissin, dry it, grind it and sieve it. Then, place 30 g of the fine powder of the bark of Albizzia julibrissin in 500 mL of ethanol solvent, extract it in a microwave reactor for 10 min, control the power of the microwave reactor to 600 W, filter it, wash the precipitate with ethanol, combine the filtrate, and dry it to obtain the bark of Albizzia julibrissin extract.

[0031] (6) Clean the Radix Rehmanniae glutinosae, dry it, grind it and sieve it, then place 30 g of the Radix Rehmanniae glutinosae fine powder in 300 mL of ethanol solvent and 500 mL of distilled water solvent, extract it in a microwave reactor for 10 min, and control the power of the microwave reactor to 500 W; filter, wash the precipitate with ethanol, combine the filtrate, and dry it to obtain the Radix Rehmanniae glutinosae extract.

[0032] (7) Wash 40 g of Scutellaria baicalensis, 70 g of White Peony Root, 15 g of Schisandra chinensis, 55 g of Astragalus, 80 g of Sophora flavescens, and 35 g of Licorice, add them to 1.5 L of distilled water, boil them, and then simmer for 30 min. After cooling, add 1.8 g of Albizzia julibrissin bark extract, 0.8 g of Rehmannia glutinosa extract, and 4 g of Lactococcus lactis fermentation broth, mix well, and obtain a fermentation complex.

[0033] Example 3

[0034] (1) Add 0.8 g of peptone, 0.15 g of casein peptide, 1.2 g of beef extract, 0.4 g of yeast extract powder, 2.5 g of glucose, 0.55 g of sodium acetate, 0.018 g of dipotassium hydrogen phosphate, 0.04 g of magnesium sulfate, 0.022 g of manganese sulfate, 0.15 g of diammonium citrate, and 0.1 g of Tween 80 to 100 g of distilled water and mix well to obtain a liquid seed culture medium.

[0035] (2) Add 1 g of yeast extract powder, 0.06 g of casein peptide, 1 g of glucose, 0.14 g of dipotassium hydrogen phosphate, 0.07 g of magnesium sulfate, 0.012 g of sodium chloride, and 0.6 g of sodium glutamate to 100 g of distilled water and mix well to obtain a fermentation medium.

[0036] (3) Add liquid seed culture medium to the flask, use an inoculation loop to take 2 loops of Lactococcus lactis and inoculate them into the liquid seed culture medium, and culture at 30° C. for 18 h to obtain a culture solution.

[0037] (4) Add 30 mL of fermentation medium to the flask, transfer 1 mL of the culture solution to the flask, and culture at 30° C. for 24 h. Centrifuge the culture solution, and then heat and boil the centrifuge solution for 5 min to obtain a Lactococcus lactis fermentation solution with a high γ-aminobutyric acid content.

[0038] (5) Clean the bark of Albizzia julibrissin, dry it, grind it and sieve it. Then, place 5 g of the fine powder of the bark of Albizzia julibrissin in 500 mL of ethanol solvent, extract it in a microwave reactor for 10 min, control the power of the microwave reactor to 400 W, filter it, wash the precipitate with ethanol, combine the filtrate, and dry it to obtain the bark of Albizzia julibrissin extract.

[0039] (6) Clean the Radix Rehmanniae glutinosae, dry it, grind it and sieve it, then place 20 g of the Radix Rehmanniae glutinosae fine powder in 200 mL of ethanol solvent and 500 mL of distilled water solvent, extract it in a microwave reactor for 20 min, and control the power of the microwave reactor to 400 W; filter, wash the precipitate with ethanol, combine the filtrate, and dry it to obtain the Radix Rehmanniae glutinosae extract.

[0040] (7) Wash 60 g of Scutellaria baicalensis, 40 g of White Peony Root, 10 g of Schisandra chinensis, 40 g of Astragalus, 30 g of Sophora flavescens, and 40 g of Licorice, add them to 2 L of distilled water, boil them, and then simmer for 60 min. After cooling, add 1.2 g of Albizzia julibrissin bark extract, 0.5 g of Rehmannia glutinosa extract, and 4 g of Lactococcus lactis fermentation broth, mix well, and obtain a fermentation complex.

[0041] Example 4

[0042] (1) Add 1 g of peptone, 0.18 g of casein peptide, 0.8 g of beef extract, 0.5 g of yeast extract, 1.5 g of glucose, 0.6 g of sodium acetate, 0.016 g of dipotassium hydrogen phosphate, 0.04 g of magnesium sulfate, 0.025 g of manganese sulfate, 0.2 g of diammonium citrate, and 0.1 g of Tween 80 to 100 g of distilled water and mix well to obtain a liquid seed culture medium.

[0043] (2) Add 0.8 g yeast extract powder, 0.08 g casein peptide, 1.4 g glucose, 0.1 g potassium dihydrogen phosphate, 0.08 g magnesium sulfate, 0.01 g sodium chloride, and 0.7 g sodium glutamate to 100 g distilled water and mix well to obtain a fermentation medium.

[0044] (3) Add liquid seed culture medium to the flask, use an inoculation loop to take one loop of Lactococcus lactis and inoculate it into the liquid seed culture medium, and culture at 30° C. for 12 h to obtain a culture solution.

[0045] (4) Add 30 mL of fermentation medium to the flask, transfer 1.5 mL of the culture solution to the flask, culture at 30° C. for 18 h, centrifuge, and then heat and boil the centrifuge for 10 min to obtain a Lactococcus lactis fermentation solution with a high γ-aminobutyric acid content.

[0046] (5) Clean the bark of Albizzia julibrissin, dry it, grind it and sieve it. Then, place 5 g of the fine powder of the bark of Albizzia julibrissin in 500 mL of ethanol solvent, extract it in a microwave reactor for 5 min, control the power of the microwave reactor to 400 W, filter it, wash the precipitate with ethanol, combine the filtrate, and dry it to obtain the bark of Albizzia julibrissin extract.

[0047] (6) Clean the Radix Rehmanniae glutinosae, dry it, grind it and sieve it, then place 50 g of the Radix Rehmanniae glutinosae fine powder in 400 mL of ethanol solvent and 500 mL of distilled water solvent, extract it in a microwave reactor for 10 min, and control the power of the microwave reactor to 800 W; filter, wash the precipitate with ethanol, combine the filtrate, and dry it to obtain the Radix Rehmanniae glutinosae extract.

[0048] (7) Wash 75 g of Scutellaria baicalensis, 40 g of White Peony Root, 6 g of Schisandra chinensis, 70 g of Astragalus, 45 g of Sophora flavescens, and 25 g of Licorice, add them to 2 L of distilled water, boil them, and then simmer for 60 min. After cooling, add 1 g of Albizzia julibrissin bark extract, 2 g of Rehmannia glutinosa extract, and 1 g of Lactococcus lactis fermentation broth, mix well, and obtain a fermentation complex.

[0049] Comparative Example 1

[0050] The difference between this comparative example and Example 1 is that the Albizzia Julibrissin Cortex extract, Rehmannia glutinosa extract and Lactococcus lactis fermentation broth are not added to the composite.

[0051] (1) Wash 45 g of Scutellaria baicalensis, 40 g of White Peony Root, 6 g of Schisandra chinensis, 60 g of Astragalus, 80 g of Sophora flavescens, and 25 g of Licorice, add them to 2 L of distilled water, boil them, and then simmer for 60 min. Cool them to obtain a complex.

[0052] Comparative Example 2

[0053] The difference between this comparative example and Example 1 is that the Albizia Julibrissin bark extract is not added to the fermentation complex.

[0054] (1) 45 g of Scutellaria baicalensis, 40 g of White Peony Root, 6 g of Schisandra chinensis, 60 g of Astragalus, 80 g of Sophora flavescens, and 25 g of Licorice were cleaned and added to 2 L of distilled water, boiled and then simmered for 60 min, cooled, and 1.5 g of Rehmannia glutinosa extract and 2 g of Lactococcus lactis fermentation liquid were added and mixed to obtain a fermentation complex.

[0055] Comparative Example 3

[0056] The difference between this comparative example and Example 1 is that the Rehmannia glutinosa extract is not added to the fermentation complex.

[0057] (1) 45 g of Scutellaria baicalensis, 40 g of White Peony Root, 6 g of Schisandra chinensis, 60 g of Astragalus, 80 g of Sophora flavescens, and 25 g of Licorice were washed and added to 2 L of distilled water, boiled and then simmered for 60 min, cooled, added with 0.3 g of Albizzia julibrissin bark extract and 2 g of Lactococcus lactis fermentation liquid, and mixed to obtain a fermentation complex.

[0058] Comparative Example 4

[0059] The difference between this comparative example and Example 1 is that no Lactococcus lactis fermentation broth is added to the composite.

[0060] (1) Wash 45 g of Scutellaria baicalensis, 40 g of White Peony Root, 6 g of Schisandra chinensis, 60 g of Astragalus, 80 g of Sophora flavescens, and 25 g of Licorice, add them to 2 L of distilled water, boil them, and then simmer for 60 min. After cooling, add 0.3 g of Albizzia julibrissin bark extract and 1.5 g of Rehmannia glutinosa extract, mix well, and obtain a composite.

[0061] 80 insomnia patients were observed, including 33 males, the oldest was 58 years old and the youngest was 22 years old; 47 females, the oldest was 61 years old and the youngest was 21 years old; they were randomly divided into 8 groups. Each group had 10 insomnia patients. Each group took the complexes of Examples 1-4 and Comparative Examples 1-4 respectively. Medication method: drink the complex once in the morning and evening, heat the complex to 50°C when drinking, 300 mL each time. Take it continuously for 30 days.

[0062] It was formulated with reference to the efficacy standards for insomnia in the "Guidelines for Clinical Research of New Chinese Medicines".

[0063] Clinical cure: Sleep time returns to normal or nighttime sleep time ≥ 6 hours, sleep is deep, and the patient is full of energy after waking up.

[0064] Significant effect: Sleep is significantly improved, sleep time is increased by 3 hours, sleep depth is increased, but the total sleep time is less than 6 hours.

[0065] Effective: Symptoms were alleviated and sleep time increased by less than 3 hours compared to before.

[0066] Ineffective: There is no significant improvement or worsening of sleep after treatment.

[0067] The test results are shown in the table below.

[0068] Table 1

[0069] As shown in Table 1, after each group took the fermentation composition of Examples 1-4, the total effective rate of treating insomnia reached 90-100%. In the composite of Comparative Example 1, no Albizzia Julibrissin extract, Rehmannia glutinosa extract, or Lactococcus lactis fermentation liquid was added, and the total effective rate of treating insomnia was only 20%. In Comparative Example 2, no Albizzia Julibrissin extract was added to the fermentation composite, and the total effective rate was only 70%. In Comparative Example 3, no Rehmannia glutinosa extract was added to the fermentation composite, and the total effective rate was only 70%. In Comparative Example 4, no Lactococcus lactis fermentation liquid was added to the composite, and the total effective rate was only 40%.

[0070] The present application is described in detail above in conjunction with the embodiments, but the present application is not limited to the above embodiments, and various changes can be made within the knowledge of ordinary technicians in the relevant technical field without departing from the purpose of the present application. In addition, the embodiments of the present application and the features in the embodiments can be combined with each other without conflict.

Claims

1. A fermented compound for improving sleep, characterized in that: The main raw materials of the fermentation complex include 40-75 parts by weight of scutellaria baicalensis, 30-70 parts by weight of white peony root, 6-15 parts by weight of schisandra chinensis, 40-70 parts by weight of astragalus, 30-80 parts by weight of sophora flavescens, 25-40 parts by weight of liquorice, 0.3-1.8 parts by weight of julibrissin bark extract, 0.5-2 parts by weight of rehmannia glutinosa extract, and 1-4 parts by weight of lactococcus lactis fermentation liquid; The preparation method of the Albizzia julibrissin bark extract comprises: cleaning the Albizzia julibrissin bark, drying, grinding and sieving, then placing the Albizzia julibrissin bark fine powder in an ethanol solvent, and controlling the ratio of the Albizzia julibrissin bark fine powder to ethanol to be (10-60) g:1 L; extracting in a microwave reactor, filtering, washing the precipitate with ethanol, combining the filtrate, and drying to obtain the Albizzia julibrissin bark extract; the power of the microwave reactor is 400-800 W, and the extraction time is 5-10 min; The preparation method of the Rehmannia glutinosa extract comprises: cleaning the Rehmannia glutinosa, drying, grinding and sieving, then placing the Rehmannia glutinosa fine powder in an ethanol solvent and a distilled water solvent, extracting in a microwave reactor, filtering, washing the precipitate with ethanol, combining the filtrate, and drying to obtain the Rehmannia glutinosa extract; the power of the microwave reactor is 400-800W, and the extraction time is 10-20min; The ratio of the Rehmannia glutinosa powder, ethanol solvent and distilled water solvent is (20-100) g: (0.4-0.8) L: 1 L.

2. A method for preparing a fermented complex for improving sleep according to claim 1, characterized in that: The preparation method comprises the following steps: cleaning scutellaria baicalensis, white peony root, schisandra chinensis, astragalus root, sophora flavescens and liquorice, adding them into distilled water, boiling and then decocting over low heat, cooling, adding julibrissin bark extract, rehmannia glutinosa extract and lactococcus lactis fermentation liquid, mixing and obtaining a fermentation complex.

3. The method for preparing a fermented complex for improving sleep according to claim 2, characterized in that: The preparation method of the Lactococcus lactis fermentation broth is: (1) adding a liquid seed culture medium to a flask, taking 1-2 rings of Lactococcus lactis with an inoculation loop and inoculating them into the liquid seed culture medium, and culturing at 30° C. for 12-18 hours to obtain a culture solution; (2) adding fermentation medium to the flask, transferring the culture solution to the flask, capping the flask, culturing at 30° C. for 18-24 h, centrifuging after culturing, and then heating and boiling the centrifuge solution for 5-10 min to obtain a Lactococcus lactis fermentation solution.

4. The method for preparing a fermented complex for improving sleep according to claim 3, characterized in that: The liquid seed culture medium comprises 100 parts by weight of distilled water, 0.8-1 parts by weight of peptone, 0.15-0.2 parts by weight of casein peptide, 0.8-1.2 parts by weight of beef extract, 0.4-0.5 parts by weight of yeast extract powder, 1.5-2.5 parts by weight of glucose, 0.5-0.6 parts by weight of sodium acetate, 0.016-0.018 parts by weight of dipotassium hydrogen phosphate, 0.04-0.05 parts by weight of magnesium sulfate, 0.02-0.025 parts by weight of manganese sulfate, 0.15-0.2 parts by weight of diamine citrate, and 0.1-0.13 g of Tween 80.

5. The method for preparing a fermented complex for improving sleep according to claim 3, characterized in that: The fermentation medium comprises 100 parts by weight of distilled water, 0.8-1 parts by weight of yeast extract powder, 0.05-0.08 parts by weight of casein peptide, 0.8-1.4 parts by weight of glucose, 0.1-0.14 parts by weight of dipotassium hydrogen phosphate, 0.07-0.08 parts by weight of magnesium sulfate, 0.01-0.012 parts by weight of sodium chloride, and 0.6-0.8 parts by weight of sodium glutamate.

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