A herbicide for controlling resistant weeds and its application

Abstract

The invention discloses a herbicide for preventing and killing resistant weeds and its application, and belongs to the technical field of herbicides. The active ingredients of the herbicide for preventing and killing resistant weeds of the present invention are bromoxynil, difluanid and bacillus subtilis microbial agent; the mass ratio of bromoxynil, difluanid and bacillus subtilis microbial agent is 150-300:11.25-90:600. The present invention combines bromoxynil, difluanid and bacillus subtilis microbial agent, which can further improve the control effect of bromoxynil and difluanid on weeds, and the synergistic effect is stronger, the survival rate of resistant weeds is lower, the use amount of the agent is greatly reduced, the drug resistance of weeds is delayed, it is environmentally friendly, and has great application prospects.

Description

Technical Field

[0001] The invention belongs to the technical field of herbicides, and in particular relates to a herbicide for controlling resistant weeds and application thereof. Background Art

[0002] At present, the control of weeds in wheat fields is mainly based on herbicide treatment. ALS inhibitors such as bensulfuron-methyl and florasulam and auxin analogue herbicides such as clofopyralid are the main agents for controlling broadleaf weeds in wheat fields. In recent years, many broadleaf weeds in wheat fields, such as Artemisia selengensis, Shepherd's purse, Glechoma longituba, Rhizoma Aesculapii, and Chickweed, have been reported to be resistant to various herbicides such as bensulfuron-methyl, florasulam, and clofopyralid, resulting in the failure of weed control in wheat fields and causing serious losses to wheat yields. The mixed use of herbicides is an effective means to delay the development of weed resistance. From the perspective of the mixed use of herbicides and biocontrol bacteria to control weeds, it is very necessary to explore the control methods of resistant broadleaf weeds in wheat fields, which can not only slow down the development of weed resistance, but also reduce the amount of herbicides used, which is beneficial to the ecological environment and human health.

[0003] Diflufenican, also known as diflufenican, chemical name N-(2,4-difluorophenyl-2-(3-trifluoromethylphenoxy)-3-pyridinecarboxamide), belongs to the benzamide herbicide. It mainly inhibits the biosynthesis of weed carotenoids, causing weed cells to rupture and chlorophyll to be destroyed, eventually leading to weed bleaching and death. Diflufenican can be used for soil treatment or stem and leaf spray, and is mainly used to control broadleaf weeds in wheat, rice, garlic and other crop fields.

[0004] Bormoxynil octanoate, also known as bromoxynil octanoate, with the chemical name 3,5-dibromo-4-octanoyloxybenzonitrile, is a selective post-emergence stem and leaf treatment agent. After being absorbed by the weed leaves, it undergoes limited transport in the body, inhibiting photosynthesis and protein synthesis, thereby causing the weeds to become chlorotic and have brown spots, ultimately causing the weeds to die. It is widely used to control broad-leaved weeds in wheat fields, corn, sorghum and other crop fields.

[0005] Bacillus subtilis is a species of Bacillus with thick cell walls, no endotoxins, and the ability to secrete a variety of proteins and hormone-like active substances. In recent years, Bacillus subtilis has played an important role in many fields. For example, it can not only shorten the fermentation cycle of organic fertilizers, but also has certain weed control activity. Summary of the invention

[0006] In view of the problems existing in the prior art, the object of the present invention is to provide a herbicide for controlling resistant weeds and its application.

[0007] In order to achieve the above object, the present invention adopts the following technical solution:

[0008] A herbicide for controlling resistant weeds, wherein the active ingredients are bromoxynil, difluanid and a Bacillus subtilis microbial agent; the mass ratio of bromoxynil, difluanid and the Bacillus subtilis microbial agent is 150-300:11.25-90:600.

[0009] Based on the above scheme, the microbial content in the Bacillus subtilis microbial agent is ≥10 8 cfu / g.

[0010] Based on the above scheme, the mass ratio of bromoxynil, difluanid and Bacillus subtilis microbial agent is 150-300:11.25-45:600.

[0011] On the basis of the above scheme, the mass of the active ingredient accounts for 1-95% of the total amount, preferably, the mass of the active ingredient accounts for 10-80% of the total amount.

[0012] Based on the above scheme, the herbicide for controlling resistant weeds further comprises an agriculturally acceptable adjuvant.

[0013] Use of the above-mentioned herbicide for controlling resistant weeds in controlling resistant weeds.

[0014] Based on the above scheme, the resistant weeds are at least one of Descurainia scabra, Glechoma longituba and Shepherd's purse.

[0015] On the basis of the above scheme, when the resistant weed is Artemisia denudata, the mass ratio of bromoxynil octanoate, difluanid and Bacillus subtilis microbial agent is 150-300:11.25-22.5:600.

[0016] On the basis of the above scheme, when the resistant weed is Galium solani, the mass ratio of bromoxynil, difluanid and Bacillus subtilis microbial agent is 150-300:22.5-45:600;

[0017] When the resistant weeds are shepherd's purse, the mass ratio of bromoxynil, difluanid and Bacillus subtilis microbial agent is 150-300:11.25-45:600.

[0018] Advantages of the technical solution of the present invention

[0019] The invention provides a ternary herbicide for preventing and controlling resistant weeds, which is composed of bromoxynil, diflufenicol and Bacillus subtilis microbial agent; the herbicide has a significant prevention and control effect on resistant weeds. When the resistant weed is Artemisia denudata, the mass ratio of bromoxynil, diflufenicol and Bacillus subtilis microbial agent is 150-300:11.25-22.5:600, which has a significant synergistic effect on its prevention and control, and greatly reduces the amount of the agent used. When the resistant weed is Galleria melongena, the mass ratio of bromoxynil, diflufenicol and Bacillus subtilis microbial agent is 150-300:22.5-45:600; the survival rate of Galleria melongena is lower than that of bromoxynil and diflufenicol when they are compounded. When the resistant weed is shepherd's purse, the mass ratio of bromoxynil, difluanid and Bacillus subtilis microbial agent is 150-300:11.25-45:600; the control effect on shepherd's purse is stronger than that of bromoxynil and difluanid, and the drug resistance of weeds can be delayed.

[0020] In summary, the ternary combination of bromoxynil, difluanid and Bacillus subtilis microbial agent has a significant control effect on resistant weeds; the addition of Bacillus subtilis microbial agent not only improves the control effect of bromoxynil and difluanid on weeds, but also has a stronger synergistic effect and a lower survival rate of resistant weeds, greatly reducing the amount of agents used, delaying the drug resistance of weeds, is environmentally friendly, and has great application prospects. DETAILED DESCRIPTION

[0021] The terms used in the present invention, unless otherwise specified, generally have the meanings commonly understood by those of ordinary skill in the art. The present invention will be further described in detail below in conjunction with specific examples and with reference to data. The following examples are intended to illustrate the present invention and are not intended to limit the scope of the present invention in any way.

[0022] The experimental methods in the following examples, unless otherwise specified, are all conventional methods, and are performed according to the techniques or conditions described in the literature in the field or according to the product instructions. The experimental materials, reagents, drugs, etc. used in the following examples, unless otherwise specified, can all be purchased through general channels.

[0023] Example

[0024] A herbicide for controlling resistant weeds in wheat fields, wherein the active ingredients are bromoxynil, difluanid and Bacillus subtilis microbial agent; the mass ratio of bromoxynil, difluanid and Bacillus subtilis microbial agent is 150-300:11.25-90:600; the microbial content in the Bacillus subtilis microbial agent is ≥10 8 cfu / g.

[0025] The Bacillus subtilis can be homemade according to the disclosed patents CN201910812781.7 and CN202011568910.1 technologies, or can be purchased through commercial channels.

[0026] Experiment on the control effect of different pesticides on weeds

[0027] Test agents: 30% bromoxynil octanoate EC, produced by Adama Huifeng (Jiangsu) Co., Ltd.; 30% difluanid SC, produced by Jiangsu Agricultural Hormone Engineering Technology Research Center Co., Ltd.; Bacillus subtilis microbial agent, produced by Hubei Qiming Bioengineering Co., Ltd., with a microbial content of ≥10 8 cfu / g.

[0028] Preparation of test agent solution: Herbicides and microbial agents are diluted with water to the required concentration.

[0029] The control effects of the pesticides and pesticide combinations listed in Table 1 on weeds such as Artemisia selengensis, Glechoma longituba, and Shepherd's purse were investigated respectively.

[0030] Application method: After the weed seeds are sown, wait until the weeds grow to 3-5 leaves, and then spray the stems and leaves. 2 The platform was sprayed with ASS-4 automatic quantitative spray system (National Agricultural Information Engineering Technology Research Center), Teejet 9503EVS fan nozzle, and the spray volume was 450L / hm 2 , spray pressure 0.275MPa, repeated 4 times.

[0031] After application, the damage symptoms of weeds (such as growth inhibition, chlorosis, deformity, etc.) were recorded in detail. After 21 days, the fresh weight of the aboveground part of each treated weed was weighed and the fresh weight control effect was calculated.

[0032] Efficacy calculation method: Colby method was used to evaluate the type of combined effect of two or three mixed drugs on weeds.

[0033] Actual weed survival rate: E (%) = (fresh weight of weeds in treatment area / fresh weight of weeds in control area) × 100, Theoretical weed survival rate E0 (%) = X × Y / 100 (binary mixture), E0 (%) = X × Y × Z / 100 2 (Three-way mixing), where X, Y, and Z represent the survival rates of weeds under different herbicide dosages.

[0034] When the E0-E value is greater than 10%, it is a synergistic effect; when it is less than -10%, it is an antagonistic effect; and between -10% and 10%, it is an additive effect.

[0035] The experimental dosage design is shown in Table 1:

[0036] Table 1 Design of dosage for weed control test of the tested agents

[0037]

[0038] The results are shown in Tables 2 to 4:

[0039] Table 2 Statistical results of the mixed use of bromoxynil, difluanid and Bacillus subtilis on Artemisia denudata

[0040] (A is bromoxynil, B is difluanid, and C is Bacillus subtilis)

[0041]

[0042] As shown in Table 2, when bromoxynil and diflufenicol are used in combination, they have a synergistic control effect on Artemisia descurainiae at dosages of 150+90, 300+45, and 300+90. When bromoxynil, diflufenicol and Bacillus subtilis are used in combination, the dosage ratios of 150+11.25, 150+22.5 and 300+22.5, which originally did not have a synergistic effect when bromoxynil and diflufenicol were used in combination, have a synergistic effect, and the combination of the three has a better control effect than the combination of the two, with a stronger synergistic effect, a lower survival rate of Artemisia descurainiae, and greatly reduces the amount of the agent used; it can be seen that Bacillus subtilis can further enhance the control effect of bromoxynil and diflufenicol on Artemisia descurainiae.

[0043] Table 3 Statistical results of the mixed use of bromoxynil, difluanid and Bacillus subtilis on the growth of Glechoma longituba

[0044] (A is bromoxynil, B is difluanid, and C is Bacillus subtilis)

[0045]

[0046] It can be seen from Table 3 that when bromoxynil and diflufenicol are used in combination, they have a synergistic control effect on galangal at dosages of 150+22.5, 150+45, 300+22.5 and 300+45. On the basis of this dosage, the preparation used in combination with Bacillus subtilis still has a synergistic control effect on galangal, and the survival rate of galangal is lower, the control effect is better, and the synergistic effect is stronger. It can be seen that Bacillus subtilis can further enhance the control effect of bromoxynil and diflufenicol on galangal.

[0047] Table 4 Statistical results of the mixed use of bromoxynil, difluanid and Bacillus subtilis on shepherd's purse

[0048] (A is bromoxynil, B is difluanid, and C is Bacillus subtilis)

[0049]

[0050] As shown in Table 4, when bromoxynil and diflufenicol are used in combination, they have a synergistic control effect on shepherd's purse at dosages of 150+45, 150+90, 300+22.5, 300+45 and 300+90; when bromoxynil, diflufenicol and Bacillus subtilis are used in combination, the dosage ratios of 150+11.25 and 300+11.25, which originally did not have a synergistic effect when bromoxynil and diflufenicol were used in combination, have a synergistic effect, and the control effect of the three combined is better than that of the two combined, the synergistic effect is stronger, and the survival rate of shepherd's purse is lower; it can be seen that Bacillus subtilis can further enhance the control effect of bromoxynil and diflufenicol on shepherd's purse.

[0051] The above is only a preferred embodiment of the present invention, and does not limit the present invention in other forms. Any technician familiar with the profession may use the above disclosed technical content to change or modify it into an equivalent embodiment with equivalent changes. However, any simple modification, equivalent change and modification made to the above embodiment according to the technical essence of the present invention without departing from the technical solution of the present invention still belongs to the protection scope of the technical solution of the present invention.

Claims

1. A herbicide for controlling resistant weeds, characterized in that: The active ingredients are bromoxynil, difluanid and Bacillus subtilis microbial agent; the microbial content in the Bacillus subtilis microbial agent is ≥10 8 cfu / g; The resistant weeds are at least one of Artemisia selengensis, Glechoma longituba and Shepherd's purse; When the resistant weed is Artemisia denudata, the mass ratio of bromoxynil, difluanid and Bacillus subtilis microbial agent is 150-300:11.25-22.5:600; When the resistant weed is Galium solani, the mass ratio of bromoxynil, difluanid and Bacillus subtilis microbial agent is 150-300:22.5-45:600; When the resistant weeds are shepherd's purse, the mass ratio of bromoxynil, difluanid and Bacillus subtilis microbial agent is 150-300:11.25-45:

600.

2. The herbicide for controlling resistant weeds according to claim 1, characterized in that: The mass of active ingredients accounts for 1-95% of the total.

3. The herbicide for controlling resistant weeds according to claim 2, characterized in that: The mass of active ingredients accounts for 10-80% of the total.

4. The herbicide for controlling resistant weeds according to claim 1, characterized in that: Agriculturally acceptable adjuvants are also included.

5. Use of the herbicide for controlling resistant weeds according to any one of claims 1 to 4 in controlling resistant weeds, characterized in that: The resistant weeds are at least one of Artemisia selengensis, Glechoma longituba and Shepherd's purse; When the resistant weed is Artemisia denudata, the mass ratio of bromoxynil, difluanid and Bacillus subtilis microbial agent is 150-300:11.25-22.5:600; When the resistant weed is Galium solani, the mass ratio of bromoxynil, difluanid and Bacillus subtilis microbial agent is 150-300:22.5-45:600; When the resistant weed is shepherd's purse, the mass ratio of bromoxynil, difluanid and Bacillus subtilis microbial agent is 150-300:11.25-45:600.

Images