A mite-killing composition and its application

Abstract

The present invention belongs to the field of pesticide acaricide technology and discloses an acaricidal composition and its use. The acaricidal composition comprises active ingredients A and B. Active ingredient A is a compound of Formula I, the structure of which is shown below: #imgabs0#. Active ingredient B is pyridabenzyl or fenpyroximate, and the mass ratio of active ingredient A to active ingredient B is 1:60 to 48:1. The acaricidal composition of the present invention exhibits significant synergistic effects against various plant-feeding mites, effectively reducing pesticide usage and alleviating environmental pollution.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide acaricide and discloses an acaricide composition and application thereof. Background Art

[0002] Fenpyroximate, commonly known as fenpyroximate, chemical name: tert-butyl 4-[[(E)-(1,3-dimethyl-5-phenoxypyrazol-4-yl)methyleneamino]oxymethyl]benzoate, CAS number: 134098-61-6. Fenpyroximate belongs to the pyrazole class of acaricides and has a good fast-acting and long-lasting effect. It is effective against various spider mites and rust mites. Its structural formula is shown below:

[0003]

[0004] Pyridaben, commonly known as pyridaben, chemical name: 2-tert-butyl-5-[(4-tert-butylphenyl)methylsulfanyl]-4-chloropyridazin-3-one, CAS number: 96489-71-3. Pyridaben is a broad-spectrum, fast-acting contact pyridazinone acaricide used to control a variety of plant-feeding mites, showing excellent efficacy throughout the entire growth period. Its structural formula is shown below:

[0005]

[0006] The compound of formula I is a new compound independently developed by our company. It has a broad insecticide spectrum and significant insecticide and acaricide effects. The structure of the compound of formula I is shown below:

[0007]

[0008] Spider mites, commonly known as red spiders, belong to the Arachnidae, Acari, Acarinae, and Tetranychidae families. They are a globally important agricultural pest, affecting fruit trees, vegetables, and flowers. They often gather on the undersides of host plant leaves, sucking sap and reducing crop photosynthesis. The leaves turn yellow and wilt, and in severe cases, the crops can die, causing significant economic losses to agricultural production. Gall mites, the smallest group of mites, are a significant pest affecting agriculture and forestry. They parasitize tender leaves, twigs, flowers, and fruit, causing abnormal plant tissue formation and posing a significant threat to fruit trees, impacting their health, productivity, and fruit quality, significantly reducing their economic value.

[0009] Integrated control measures are often used to control field mite populations, with chemical control being the primary and most commonly used method. To clarify the efficacy of compound I in combination with fenpyroximate or pyridabenz, we investigated the indoor activity and field efficacy of different combinations and ratios of these agents against target mites, Tetranychus cinnabarinus, Panonychus citri, and Trichoderma citri. The aim is to provide a scientific basis for the selection and use of chemical agents for field mite control. Summary of the Invention

[0010] In order to address the deficiencies in the prior art, the present invention provides a mite-killing composition and its application. The mite-killing composition of the present invention has excellent control effects on herbivorous mites, especially effective against pest mites such as spider mites, two-spotted spider mites, citrus rust mites, etc., effectively reducing the use of pesticides and alleviating pollution to the environment.

[0011] In order to achieve the above object, the present invention adopts the following technical solution: a mite-killing composition, wherein the active ingredients of the mite-killing composition include active ingredient A and active ingredient B, wherein the active ingredient A is a compound of formula I, and the structure of the compound of formula I is shown below: The active ingredient B is pyridabenz or fenpyroximate, and the mass ratio of the active ingredient A to the active ingredient B is 1:60 to 48:1, or any value within the above numerical range;

[0012] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:60 to 40:1, or any value within the above numerical range;

[0013] Furthermore, the active ingredient B is pyridaben, and the mass ratio of the compound of formula I to pyridaben is 1:32 to 40:1, or any value within the above numerical range;

[0014] The active ingredient B is fenpyroximate, and the mass ratio of the compound of formula I to fenpyroximate is 1:60 to 38:1, or any value within the above numerical range;

[0015] Furthermore, the active ingredient B is pyridaben, and the mass ratio of the compound of formula I to pyridaben is 1:30 to 25:1, or any value within the above numerical range;

[0016] The active ingredient B is fenpyroximate, and the mass ratio of the compound of formula I to fenpyroximate is 1:42 to 38:1, or any value within the above numerical range;

[0017] Furthermore, the active ingredient B is pyridaben, and the mass ratio of the compound of formula I to pyridaben is 1:25 to 25:1, or any value within the above numerical range;

[0018] The active ingredient B is fenpyroximate, and the mass ratio of the compound of formula I to fenpyroximate is 1:36 to 38:1, or any value within the above numerical range;

[0019] Furthermore, the total weight of the acaricidal composition is 100 wt%, and the total weight of the active ingredient A and the active ingredient B accounts for 0.5% to 80% of the total weight of the acaricidal composition;

[0020] Furthermore, the composition contains, in addition to the active ingredient, auxiliary ingredients permitted in pesticides, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers;

[0021] The wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium lauryl sulfate, sodium dioctyl sulfosuccinate, α-olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, soapberry powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0022] The dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0023] The emulsifier is selected from one or more of calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0024] The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0025] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0026] The antifreeze agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0027] Defoaming agent selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of fatty alcohols; and / or

[0028] The solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel, solvent oil, vegetable oil, vegetable oil derivatives and water; and / or

[0029] The preservative is selected from one or more of propionic acid, sodium propionic acid, sorbic acid, sodium sorbic acid, potassium sorbic acid, benzoic acid, sodium benzoic acid, sodium p-hydroxybenzoic acid, methyl p-hydroxybenzoate, kasone and 1,2-benzisothiazolin-3-one; and / or

[0030] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc, montmorillonite and starch; and / or

[0031] Synergists are selected from synergist, piperonyl butoxide; and / or

[0032] The carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives;

[0033] Furthermore, the acaricide composition is prepared into a formulation permitted by pesticides, and the formulation is a solid formulation or a liquid formulation;

[0034] Furthermore, the solid preparation includes powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules;

[0035] The liquid preparations include soluble solutions, soluble gels, oils, film-spreading oils, emulsifiable concentrates, latexes, dispersible solutions, ointments, aqueous emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspoemulsions, microcapsule suspension-suspension concentrates, microcapsule suspension-water emulsions, or microcapsule suspension-suspoemulsions;

[0036] Furthermore, the solid preparation is a water-dispersible granule or a wettable powder, and the liquid preparation is a suspension, a microemulsion, an emulsifiable concentrate, an aqueous emulsion, or a dispersible oil suspension.

[0037] The present invention also discloses the use of the acaricidal composition for preventing and controlling herbivorous mites.

[0038] Furthermore, the phytophagous pest mites are from the Tetranychidae family and the Mylidae family.

[0039] The beneficial effects of the present invention are as follows:

[0040] 1) The acaricidal composition of the present invention rationally combines compounds with different mechanisms of action, and has a significant synergistic effect on herbivorous mites;

[0041] 2) The acaricide composition of the present invention effectively reduces the dosage of pesticides, reduces the pollution of pesticides to the environment, and reduces pesticide residues on crops. DETAILED DESCRIPTION

[0042] In order to make the technical solutions, objectives and advantages of the present invention more clearly understood, the present invention is described with reference to the following specific embodiments. However, the present invention can be implemented in various forms and should not be limited to the embodiments described herein.

[0043] Preparation example:

[0044] Preparation Example 1: 17% Formula I compound·pyridabenzyl suspension (1:16)

[0045] Formula composition: 1% compound of formula I, 16% pyridaben, 2% phenethylphenol polyoxyethylene polyoxypropylene ether, 3% alkylaryl polyoxyethylene ether polyoxypropylene ether, 3% tristyrylphenol ethoxylate phosphate, 1% sodium polycarboxylate, 2% magnesium aluminum silicate, 0.1% carboxyethyl cellulose, 1% sodium benzoate, 5% glycerol, 0.5% silicone oil, and deionized water to make up the balance;

[0046] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, water is added and mixed evenly, and the suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0047] Preparation Example 2: 27% Formula I compound·pyridabenzyl emulsifiable concentrate (1:8)

[0048] Formula composition: 3% compound of formula I, 24% pyridabenz, 18% propylene glycol methyl ether, 15% fatty alcohol polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 12% DMF, and methyl oleate makes up the balance;

[0049] Preparation method: according to the formula ratio, the measured active ingredients, solvent and cosolvent are added into a mixing kettle and stirred to dissolve them, then the emulsifier is added and the balance is supplemented with the remaining solvent, and the mixture is stirred evenly in a stirring kettle. After filtering, the desired emulsifiable concentrate of the present invention is obtained.

[0050] Preparation Example 3: 15% Formula I compound·pyridabenzyl wettable powder (1:2)

[0051] Formula composition: 5% compound of formula I, 10% pyridaben, 4% sodium lignin sulfonate, 5% naphthalene sulfonate formaldehyde condensate, 2% pulverized powder BX, 6% white carbon black, and kaolin to make up the balance;

[0052] Preparation method: According to the formula ratio, the active ingredients, dispersants, wetting agents and fillers are mixed, uniformly stirred in a stirring kettle, and pulverized and mixed uniformly multiple times in a jet mill to prepare the wettable powder of the composition of the present invention.

[0053] Preparation Example 4: 10% Formula I Compound·Pyridabenzyl Microemulsion (4:1)

[0054] Formula composition: 8% compound of formula I, 2% pyridaben, 12% glycerol fatty acid ester polyoxyethylene ether, 1% polyoxyethylene sorbitan monooleate, 4% styrylphenol polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 2% sodium sulfate of fatty alcohol polyoxyethylene ether, 20% cyclohexanone, 0.5% organosilicon defoamer, and deionized water to make up the balance;

[0055] Preparation method: After completely dissolving the active ingredient in the solvent according to the formula ratio, add an emulsifier to make an oil phase, add a dispersant, deionized water, etc. and stir evenly to make an aqueous phase; add the oil phase to the aqueous phase and stir evenly, shear at high speed until the particle size meets the requirements, add a defoaming agent and stir evenly to obtain the microemulsion product.

[0056] Preparation Example 5: 18% Formula I compound·pyridabenzyl water dispersible granules (8:1)

[0057] Formula composition: 16% compound of formula I, 2% pyridaben, 3% styrenephenol polyoxyethylene ether sulfate, 10% naphthalenesulfonate formaldehyde condensate, 5% sodium polycarboxylate, 3% opening powder BX, 10% white carbon black, and kaolin makes up the balance.

[0058] Preparation method: According to the formula ratio, the active ingredient is added to the carrier, and surfactants and other functional additives are added thereto, mixed, and after air flow grinding, 10-25% water is added, and then kneading, granulation, drying, and screening are carried out to obtain a water-dispersible granule product; or the crushed powder is sprayed with water in a boiling granulator, granulated, dried, and then sieved to obtain the product.

[0059] Preparation Example 6: 21% Formula I compound·pyridabenzyl dispersible oil suspension (20:1)

[0060] Formula composition: 20% compound of formula I, 1% pyridaben, 5% alkylphenol polyoxyethylene ether, 10% sorbitan oleate polyoxyethylene ether, 1% calcium dodecylbenzenesulfonate, 1% sodium polycarboxylate, 1% naphthalenesulfonate formaldehyde condensate, 0.3% organic bentonite, and soybean oil makes up the balance;

[0061] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reactor in sequence, oil is added and mixed evenly, and the dispersible oil suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0062] Preparation Example 7: 13.6% Formula I Compound·Pyridabenzyl Emulsion in Water (16:1)

[0063] Formula composition: 12.8% compound of formula I, 0.8% pyridaben, 5% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 3% polyoxyethylene sorbitan monooleate, 12% cyclohexanone, 0.2% xanthan gum, 5% ethylene glycol, 1% urea, 0.5% sodium benzoate, 0.05% organosilicon defoamer, and deionized water to make up the balance;

[0064] Preparation method: After completely dissolving the active ingredient in the solvent according to the formula ratio, add the emulsifier to form the oil phase; stir the dispersant, antifreeze, deionized water, etc. to form the water phase; add the oil phase to the water phase and stir evenly, shear at high speed until the particle size meets the requirements, add the defoaming agent and stir evenly to obtain the water emulsion product.

[0065] Preparation Example 8: 24% Formula I compound·fenpyroximate suspension (15:1)

[0066] Formula composition: 22.5% compound of formula I, 1.5% fenpyroximate, 2% phenylethylphenol polyoxyethylene polyoxypropylene ether, 5% alkylaryl polyoxyethylene ether polyoxypropylene ether, 2% tristyrylphenol ethoxylate phosphate, 1% sodium polycarboxylate, 2% magnesium aluminum silicate, 0.2% carboxyethyl cellulose, 1% sodium sorbate, 5% glycerol, 0.5% silicone oil, and deionized water to make up the balance;

[0067] Preparation method: Same as Preparation Example 1.

[0068] Preparation Example 9: 18% Formula I compound·pyraclostrobin emulsifiable concentrate (8:1)

[0069] Formula composition: 16% compound of formula I, 2% fenpyroximate, 15% DMF, 15% Guerbet alcohol polyoxyethylene ether, 3% calcium dodecylbenzenesulfonate, 22% propylene carbonate, and xylene to make up the balance;

[0070] Preparation method: Same as Preparation Example 2.

[0071] Preparation Example 10: 32% Formula I compound·fenpyroximate wettable powder (1:15)

[0072] Formula composition: 2% compound of formula I, 30% fenpyroximate, 5% sodium lignin sulfonate, 5% sodium alkylphenol polyoxyethylene ether sulfonate, 2% sodium naphthalene sulfonate, 2% sodium lauryl sulfate, 8% attapulgite, and kaolin makes up the balance;

[0073] Preparation method: Same as Preparation Example 3.

[0074] Preparation Example 11: 9% Formula I compound·fenpyroximate microemulsion (1:8)

[0075] Formula composition: 1% compound of formula I, 8% fenpyroximate, 15% xylene, 20% cyclohexanone, 15% glycerol fatty acid ester polyoxyethylene ether, 5% EO-PO block copolymer, 1% sodium octylphenol polyoxyethylene ether sulfonate, 5% ethylene glycol, 0.1% silicone defoamer, and deionized water to make up the balance;

[0076] Preparation method: same as Preparation Example 4.

[0077] Preparation Example 12: 24% Formula I compound·fenpyroximate water dispersible granules (1:3)

[0078] Formula composition: 6% compound of formula I, 18% fenpyroximate, 7% sodium lignin sulfonate, 5% sodium alkyl polyoxyethylene ether sulfonate, 2% flaking powder BX, 3% sodium polycarboxylate, 10% ammonium sulfate, and kaolin makes up the balance;

[0079] Preparation method: Same as Preparation Example 5.

[0080] Preparation Example 13: 20% Formula I compound·fenpyroximate dispersible oil suspension (4:1)

[0081] Formula composition: 16% compound of formula I, 4% pyraclostrobin, 15% phenethylphenol polyoxyethylene polyoxypropylene ether, 5% alkylphenol polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 1% silicon dioxide, 0.5% organic bentonite, 20% 200# solvent oil, and methyl oleate to make up the balance;

[0082] Preparation method: same as Preparation Example 6.

[0083] Preparation Example 14: 7% Formula I compound·fenpyroximate aqueous emulsion (6:1)

[0084] Formula composition: 6% compound of formula I, 1% pyraclostrobin, 2% arylphenol polyoxyethylene ether phosphate, 6% alkylaryl polyoxyethylene ether polyoxypropylene ether, 20% cyclohexanone, 5% ethylene glycol, 0.05% organosilicon defoamer, and deionized water to make up the balance;

[0085] Preparation method: Same as Preparation Example 7.

[0086] Indoor biological activity test

[0087] Example 1: Indoor biological activity test on spider mites

[0088] Test basis: The test refers to NY / T 1154.12-2008 "Indoor biological activity test of pesticides - Insecticides Part 12: Spider mite slide immersion method"

[0089] Test targets: Tetranychus cinnabarinus and Panonychus citri, and female adult mites of the above pest mites were selected.

[0090] Test agents: compound of formula I, pyridabenz, and fenpyroximate technical;

[0091] Preparation of the drug: Dissolve the above raw drugs in a suitable solvent, then dilute with 0.1% Tween 80 aqueous solution, and set 5 mass concentration gradients according to the activity of the drug.

[0092] Test method: Cut double-sided tape into 2 cm lengths and stick them to one end of a slide. Then select healthy mites and stick their backs to the double-sided tape, 30 mites per slide. Place them in a container padded with a wet sponge, cover with a lid, and place at (25±1)°C. After 2 hours, examine under a microscope, remove dead and injured individuals, and replenish 30 mites per slide.

[0093] Chemical treatment: Immerse the slide in each treatment solution and gently shake for 5 seconds. Remove the slide and remove the excess solution with absorbent paper. Place the slide on a white porcelain plate lined with a damp sponge. Cover with a light-transmitting plastic film and place at (25 ± 1)°C for observation. Repeat four times for each treatment, and include a blank control with no chemical.

[0094] Experimental investigation: 48 hours after treatment, check the death of test insects, record the total number of insects and the number of dead insects, and calculate the mortality rate.

[0095] Data statistics and analysis:

[0096] Based on the survey data, the adjusted mortality rate of each treatment was calculated according to the following formula.

[0097]

[0098] Where:

[0099] P——mortality rate, in percentage (%);

[0100] K——indicates the number of dead insects, the unit is head;

[0101] N——represents the total number of insects processed, in heads.

[0102]

[0103] Where:

[0104] P1——adjusted mortality rate, in percentage (%);

[0105] P t ——Treatment mortality rate, expressed in percentage (%);

[0106] P0 - blank control mortality rate, in percentage (%).

[0107] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the correction mortality formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0108] Analyze using the DPS statistical analysis system to determine the LC 50 The activity of the test agent on the biological test material is evaluated by the value.

[0109] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0110]

[0111] Where:

[0112] ATI - measured toxicity index of mixture;

[0113] S——LC of standard acaricide 50 , the unit is milligrams per liter (mg / L);

[0114] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0115] TTI=TI A ×P A +TI B ×P B

[0116] Where:

[0117] TTI – Theoretical Toxicity Index of Mixtures;

[0118] TI A ——Agent toxicity index;

[0119] P A ——The percentage of agent A in the mixture, in percentage (%);

[0120] TI B ——Toxicity index of agent B;

[0121] P B ——The percentage of agent B in the mixture, in percentage (%).

[0122]

[0123] Where:

[0124] CTC – Co-toxicity coefficient;

[0125] ATI - measured toxicity index of mixture;

[0126] TTI - Theoretical Toxicity Index of Mixture.

[0127] A co-toxicity coefficient (CTC) of ≥120 indicates a synergistic effect; a CTC of ≤80 indicates an antagonistic effect; and a CTC of 80 < CTC <120 indicates an additive effect. See the table below for indoor activity tests:

[0128] Table 1 Results of indoor biological activity test of compound of formula I and fenpyroximate against Tetranychus cinnabarinus

[0129] Test drug Virulence regression equation Correlation coefficient R <![CDATA[LC 50 (mg / L)]]> Co-toxicity coefficient Compounds of formula I y=3.1850+1.4629x 0.9983 17.4049 / Fenpyraclostrobin y=2.8276+1.4517x 0.9913 31.3647 / 1:60 y=2.9663+1.4529x 0.9988 25.1026 123.324 1:48 y=2.9868+1.4823x 0.9998 22.8123 135.276 1:32 y=3.3741+1.2364x 0.9970 20.6568 148.234 1:16 y=3.3576+1.3169x 0.9925 17.6687 169.518 1:8 y=3.4999+1.2425x 0.9958 16.1192 178.658 1:2 y=3.3775+1.4053x 0.9996 14.2761 173.354 2:1 y=3.6745+1.1965x 0.9889 12.8171 159.450 8:1 y=3.5459+1.3263x 0.9863 12.4847 146.663 16:1 y=3.6790+1.1723x 0.9982 13.3927 133.452 32:1 y=3.5998+1.2292x 0.9988 13.7768 128.062 48:1 y=3.5075+1.2528x 0.9969 15.5355 113.060

[0130] Table 2 Results of indoor biological activity test on Tetranychus cinnabarinus with compound of formula I and pyridabenzyl

[0131] Test drug Virulence regression equation Correlation coefficient R <![CDATA[LC 50 (mg / L)]]> Co-toxicity coefficient Compounds of formula I y=3.1850+1.4629x 0.9983 17.4049 / Pyridaben y=5.4606+1.5668x 0.9959 0.5082 / 1:50 y=5.5110+1.4749x 0.9977 0.4503 115.048 1:30 y=5.5560+1.4564x 0.9851 0.4152 126.356 1:25 y=5.5290+1.1748x 0.9943 0.3546 148.875 1:15 y=5.6600+1.3420x 0.9970 0.3223 167.864 1:8 y=5.7169+1.3918x 0.9928 0.3054 186.525 6:1 y=4.8024+1.1532x 0.9854 1.4836 204.036 10:1 y=4.5937+1.2053x 0.9884 2.1732 199.099 15:1 y=4.1933+1.5040x 0.9990 3.4385 164.450 20:1 y=4.1980+1.1537x 0.9891 4.9562 135.943 25:1 y=3.9201+1.3890x 0.9979 5.9901 127.508 40:1 y=4.0436+1.0730x 0.9933 7.7871 123.422

[0132] Indoor toxicity bioactivity test results (see Table 1-2) show that the compound of Formula I exhibits a significant synergistic effect with either pyraclostrobin or pyridabenzain against Tetranychus cinnabarin within a reasonable ratio range. When the mass ratio of the compound of Formula I to pyraclostrobin is 1:60 to 32:1, the co-toxicity coefficient against Tetranychus cinnabarin exceeds 120, demonstrating a synergistic effect. When the mass ratio is 1:48 to 16:1, the co-toxicity coefficient against Tetranychus cinnabarin exceeds 130, demonstrating a significant synergistic effect. When the mass ratio is 1:32 to 8:1, the co-toxicity coefficient against Tetranychus cinnabarin exceeds 140, demonstrating a significant synergistic effect. When the mass ratio of the compound of Formula I to pyridabenzain is 1:30 to 40:1, the co-toxicity coefficient against Tetranychus cinnabarin exceeds 120, demonstrating a synergistic effect. When the mass ratio is 1:25 to 20:1, the co-toxicity coefficient against Tetranychus cinnabarin exceeds 130, demonstrating a significant synergistic effect.

[0133] Table 3 Results of indoor biological activity test of compound of formula I and fenpyroximate against Panonychus citri

[0134] Test drug Virulence regression equation Correlation coefficient R <![CDATA[LC 50 (mg / L)]]> Co-toxicity coefficient Compounds of formula I y=2.0848+1.5613x 0.9990 73.6361 / Fenpyraclostrobin y=4.5681+1.6474x 0.9984 1.8290 / 1:42 y=4.7240+1.5515x 0.9944 1.5062 124.249 1:36 y=4.7928+1.5243x 0.9975 1.3675 137.368 1:18 y=4.8568+1.3903x 0.9951 1.2676 152.095 1:9 y=4.8839+1.5263x 0.9993 1.1913 170.119 9:1 y=3.7709+1.4395x 0.9960 7.1429 209.276 18:1 y=3.3656+1.4720x 0.9971 12.8901 186.301 26:1 y=3.2313+1.3976x 0.9980 18.4312 162.797 38:1 y=2.9307+1.4682x 0.9995 25.6709 142.946 40:1 y=2.8518+1.4471x 0.9988 30.5176 123.260

[0135] Table 4 Results of indoor biological activity test on compound of formula I and pyridabenzyl against Panonychus citri

[0136]

[0137]

[0138] Indoor toxicity bioactivity test results (see Tables 3-4) show that the compound of Formula I exhibits a significant synergistic effect with either pyridabenza or pyridabenza within a reasonable ratio against Panonychus citri. When the mass ratio of the compound of Formula I to pyridabenza is 1:42 to 40:1, the co-toxicity coefficient against Panonychus citri is greater than 120, demonstrating a synergistic effect. When the mass ratio is 1:36 to 38:1, the co-toxicity coefficient against Panonychus citri is greater than 130, indicating a significant synergistic effect. When the mass ratio is 1:18 to 38:1, the co-toxicity coefficient against Panonychus citri is greater than 140, demonstrating a significant synergistic effect. The mass ratio of the compound of formula I to pyridabenzain is 1:32 to 40:1, and the co-toxicity coefficient to citrus panonychus mite is greater than 120, showing a synergistic effect; the mass ratio of the compound of formula I to pyridabenzain is 1:16 to 32:1, and the co-toxicity coefficient to citrus panonychus mite is greater than 130, and the synergistic effect is obvious; the mass ratio of the compound of formula I to pyridabenzain is 1:8 to 16:1, and the co-toxicity coefficient is greater than 140, and the synergistic effect is significant.

[0139] Field efficacy trials

[0140] Example 2: Field test on controlling strawberry spider mites

[0141] Test location: In the strawberry greenhouse of Xinggongshan Village, Dongpu Town, Shaoxing City, Zhejiang Province. The spider mites in the test site were a mixed population of two-spotted spider mites and cinnabarinus spider mites.

[0142] Experimental crop: Strawberry (Red Beauty).

[0143] Experimental design: The experiment set up 5 pesticide treatments and a blank control, a total of 6 treatments, each treatment area was 20m 2 Each treatment was repeated 4 times, and the experimental plots were arranged in random blocks.

[0144] Application method: The application time is March 11, 2023. Use a Gongnong-16 backpack sprayer to evenly spray the entire strawberry plant.

[0145] Survey Method: The base mite population was surveyed before application, and the number of live mites was surveyed 3, 7, and 14 days after application. Five sampling points were taken in the plot, with five leaves fixed at each point, for a total of 25 leaves surveyed.

[0146] Calculation method of drug efficacy:

[0147]

[0148]

[0149] Test results and analysis:

[0150] Table 5 Results of field trials on control of strawberry spider mites

[0151]

[0152] Safety investigation results: During the whole experiment, observations were made at irregular intervals, and the growth of strawberries in the treatment areas and the control areas were consistent, as were other pests and diseases and other organisms, with no abnormalities. This indicates that the safety of each treatment dosage in this experiment is good.

[0153] Results of the control efficacy investigation: The results of the field efficacy test showed that the acaricidal composition of the present invention can be used to control strawberry spider mites. 14 days after application, the compound treatment group still maintained a good control effect.

[0154] Example 2: Field test on controlling citrus mites

[0155] Test location: This test was conducted in Dagang Town, Nansha District, Guangzhou City, Guangdong Province. The test site has flat terrain, medium fertility, and convenient irrigation and drainage.

[0156] Experimental crops: citrus.

[0157] Test targets: Citrus rust tick, Citrus citrus mites.

[0158] Experimental design: The experiment set up 6 treatments, 4 replications, and a total of 24 plots, with 2 citrus trees in each plot. The plots were arranged in random blocks, and isolation areas were set between them to prevent pesticide drift or other factors that affect the test results.

[0159] Application time and method: The experiment was carried out once on August 30, 2023, using a Gongnong-16 backpack sprayer. The amount of pesticide applied was sufficient to moisten the leaves and fruits, with a slight drop of pesticide from the leaves.

[0160] Test survey: Survey two trees per plot. Mark two infested leaves at the same tip stage on the east, south, west, north, and center sides of the tree. Survey two visual fields and record the number of live mites. Survey the base mite population before spraying and again 3, 10, and 20 days after spraying to count the number of live mites.

[0161] Safety investigation: During the entire efficacy test, the growth of citrus fruits in each drug treatment area and the clear water control area was observed at irregular intervals.

[0162] Calculation method of drug efficacy:

[0163]

[0164]

[0165] Test results and analysis:

[0166] Table 6 Results of field trials on citrus mites

[0167]

[0168] Safety: During the field test, the leaf color, plant shape, etc. of citrus plants in the different pesticide treatment areas and the blank control area were observed. No pesticides were found to cause phytotoxicity to the citrus plants, nor were there any effects on promoting or inhibiting citrus growth. This shows that the various dosages of the pesticides selected in this test are relatively safe for citrus trees.

[0169] The results of the field efficacy test show that the combination of the compound of formula I with cypermethrin or pyridaben has a good effect on citrus mites. The control effect on citrus mites is 89.59% and 92.85% 3 days after treatment, 94.09% and 95.68% 10 days after treatment, and 95.94% and 98.25% 20 days after treatment, which are significantly higher than the single-dose control.

[0170] Although the present application describes specific embodiments in detail by way of example, the disclosure of the present application may adopt various modifications and alternative forms. However, it should be understood that the disclosure of the present application is not limited to the specific forms disclosed. On the contrary, the disclosure of the present application covers all modifications, equivalents and alternative forms within the scope of the disclosure of the present application, and the scope of the present application is limited by the appended claims and their legal equivalents.

Claims

1. A mite-killing composition, characterized in that: The active ingredients in the acaricidal composition include active ingredient A and active ingredient B. The active ingredient A is a compound of formula I, and the structure of the compound of formula I is shown below: (Formula I), The active ingredient B is pyridaben, and the mass ratio of the compound of formula I to pyridaben is 1:32-40:

1.

2. The acaricidal composition according to claim 1, characterized in that The mass ratio of the compound of formula I to pyridaben is 1:30 to 25:

1.

3. The acaricidal composition according to claim 1, characterized in that The total weight of the acaricidal composition is 100 wt %, and the total weight of the active ingredient A and the active ingredient B accounts for 0.5% to 80% of the total weight of the acaricidal composition.

4. The acaricidal composition according to claim 1, characterized in that In addition to the active ingredients, the acaricidal composition also contains auxiliary ingredients permitted in pesticides, wherein the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

5. The acaricidal composition according to claim 1, characterized in that The acaricide composition is prepared into a formulation form permitted by pesticides, and the formulation form is a solid preparation or a liquid preparation.

6. The acaricidal composition according to claim 5, characterized in that The solid preparation is a water-dispersible granule or a wettable powder, and the liquid preparation is a suspension, a microemulsion, an emulsifiable concentrate, an aqueous emulsion, or a dispersible oil suspension.

7. Use of the acaricidal composition according to any one of claims 1 to 6 for controlling phytophagous pest mites, characterized in that: The herbivorous pest mites are Tetranychus cinnabarinus and Panonychus citri.

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