A composite hydrogel for pet wound care and methods of making and products thereof

Abstract

The present application provides a kind of pet wound care compound hydrogel and its preparation method, raw materials include: lignin-copper gluconate complex, sodium hyaluronate, propylene glycol, aloe extract, wherein, in mass percent, copper ion in lignin-copper gluconate complex is added 0.01% to 0.1%.The compound hydrogel is alcohol-free, preservative-free, non-toxic and harmless, edible, more easily accepted by pets and combined with wound care, low sensitivity and mild, no irritation to pet skin.

Description

Technical Field

[0001] The present invention relates to the technical field of biomedicine, and in particular to a composite hydrogel for pet wound care, a preparation method thereof, and a product thereof. Background Art

[0002] The skin is the largest organ in an animal's body and its most important protective layer. Therefore, it is often subject to external aggressions, resulting in various wounds. Due to their unique lifestyles, wounds in domestic pets like cats and dogs are slow to heal. Long-term exposure to air can lead to bacterial and fungal infections, which can hinder the healing process.

[0003] Candida albicans is a fungus that infects the mouth, digestive tract, and skin of pets. When infected, pets experience itching, redness, and hair loss. Under normal circumstances, a pet's immune system controls the number of Candida albicans, keeping it within a normal range and preventing illness. However, when the immune system is compromised, especially when the skin is damaged or wounded, Candida albicans can overgrow, causing infection and hindering wound healing.

[0004] Common pet wound care products currently on the market include disinfectants, antibiotic ointments, dressings, and wound patches. These products can be toxic, requiring care to prevent pets from licking or biting the wound to avoid accidental poisoning. They can also be irritating to pets' skin, potentially causing allergic reactions. These reactions can lead to discomfort or even rejection, disrupting wound healing. They can also develop drug resistance, resulting in reduced efficacy with prolonged or excessive use, which can affect wound healing.

[0005] In current antimicrobial hydrogel preparation technologies, metal ions, particularly copper ions, demonstrate significant antimicrobial potential. Through their unique chemical mechanism of action, copper ions can disrupt the cell membrane structure of microorganisms, thereby interfering with their metabolic processes and ultimately leading to cell death. This effect endows copper ions with the ability to effectively kill a wide range of bacteria and fungi. However, it is worth noting that high concentrations of copper sulfate may cause adverse irritation and damage to the skin. Summary of the Invention

[0006] In order to solve the problems existing in the prior art, the present invention provides a composite hydrogel for pet wound care, a preparation method and a product thereof. The composite hydrogel is alcohol-free, preservative-free, non-toxic and harmless, and edible. Pets are more easily accepted and cooperate with wound care. It is hypoallergenic and mild and does not irritate pets' skin.

[0007] To achieve the above objectives, the present invention provides the following technical solution: a composite hydrogel for pet wound care, the raw materials comprising: lignin-copper gluconate complex, sodium hyaluronate, propylene glycol, and aloe extract, wherein the copper ion addition amount in the lignin-copper gluconate complex is 0.01% to 0.1% by mass.

[0008] Furthermore, in terms of mass percentage, 0.01% to 10% of sodium hyaluronate, 0.1% to 10% of propylene glycol, and 0.01% to 5% of aloe vera extract are added to every 100 mL of lignin-copper gluconate complex, and the pH value is adjusted to a range of 5.8 to 7.2 to obtain a complex hydrogel for pet wound care.

[0009] Furthermore, when preparing the lignin-copper gluconate complex, equal volumes of lignin solution and copper gluconate solution are mixed, and the lignin-copper gluconate complex is obtained after reaction.

[0010] The present invention also provides a method for preparing a composite hydrogel for pet wound care, and the specific preparation steps are as follows:

[0011] S1. Take 100 mL of lignin-copper gluconate complex, and add 0.01% to 0.1% copper ions in the lignin-copper gluconate complex by mass percentage;

[0012] S2. Sodium hyaluronate, propylene glycol and aloe extract are sequentially added to the lignin-copper gluconate complex, and the pH value is adjusted to be neutral or weakly acidic to obtain a complex hydrogel for pet wound care.

[0013] Furthermore, in S1, equal volumes of the lignin solution and the copper gluconate solution are mixed, and a lignin-copper gluconate complex is obtained after the reaction.

[0014] Furthermore, in S2, in terms of mass percentage, 0.01% to 10% of sodium hyaluronate, 0.1% to 10% of propylene glycol, and 0.01% to 5% of aloe vera extract are added to every 100 mL of the lignin-copper gluconate complex.

[0015] Furthermore, in S2, the pH value is adjusted to be in the range of 5.8 to 7.2.

[0016] The present invention also provides a product for treating fungal infections, comprising the above-mentioned composite hydrogel for pet wound care or the composite hydrogel for pet wound care prepared by the above-mentioned preparation method.

[0017] Furthermore, the fungal infection is caused by Candida albicans.

[0018] Furthermore, the fungal infection is an epidermal fungal infection.

[0019] Compared with the prior art, the present invention has at least the following beneficial effects:

[0020] The present invention provides a composite hydrogel for pet wound care, comprising a lignin-copper gluconate complex. Lignin can destroy microbial cell walls through both physical and chemical actions, thereby exhibiting certain antibacterial properties. More importantly, lignin exhibits excellent biocompatibility, a property that prevents it from causing irritation or discomfort when in contact with pet skin and wounds. Lignin undergoes a chemical cross-linking reaction with copper gluconate to form a lignin-copper gluconate complex. This complex not only combines the respective advantages of lignin and copper ions but also significantly enhances the overall antibacterial properties through their synergistic effect. Specifically, the porous structure and polymer network of lignin provide a stable physical support for the complex, contributing to the formation of a stable hydrogel system. Furthermore, lignin can adsorb and slowly release copper ions, a property that not only enhances the durability of the antibacterial effect but also effectively reduces the potential side effects of copper ions. The synergistic antibacterial effect of the lignin-copper gluconate complex effectively kills Candida albicans, significantly enhancing the antibacterial properties. The addition of sodium hyaluronate, which promotes tissue regeneration and wound healing, propylene glycol for moisturizing properties, and aloe vera extract for soothing and anti-inflammatory properties not only enhances the hydrogel's moisturizing and soothing properties but also promotes wound healing, making it particularly suitable for pet wound care. This optimized composite hydrogel offers significant advantages over simple copper gluconate hydrogel, making it an ideal pet wound care product. It is alcohol-free, fragrance-free, preservative-free, non-toxic, and edible.

[0021] The preparation method of the present invention has clear steps, readily available raw materials, does not require complex equipment, and is suitable for large-scale production. The desired composite hydrogel can be obtained by simple mixing and pH adjustment, thereby reducing production costs and improving production efficiency.

[0022] The composite hydrogel for pet wound care of the present invention has shown good therapeutic effects for epidermal fungal infections caused by fungi such as Candida albicans. Its unique antibacterial mechanism can kill fungi efficiently while avoiding excessive irritation and damage to the pet's skin, providing a safe and effective treatment plan for pets. In addition to the direct antibacterial effect, the treatment product also provides comprehensive wound care for pets through the synergistic effect of sodium hyaluronate, propylene glycol and aloe extract. These ingredients help to reduce inflammation, promote tissue repair and moisturize, accelerate the wound healing process, and improve the recovery speed and comfort of pets. Because the treatment product is alcohol-free, preservative-free, hypoallergenic, mild and edible (although not recommended), pets will not have strong resistance or discomfort during use. This helps owners better perform wound care for their pets and improves the compliance and effectiveness of treatment. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a morphological diagram of the composite hydrogel for pet wound care prepared in Example 4 of the present invention;

[0024] Figure 2 The Candida albicans drop plate diagram and survival rate statistical diagram of the composite hydrogel for pet wound care prepared in Examples 1 to 4 of the present invention;

[0025] Figure 3 This is a graph showing the changes in the wound area of ​​mice caused by the composite hydrogels for pet wound care prepared in Examples 1 to 4 of the present invention. DETAILED DESCRIPTION

[0026] This description clarifies the understanding and interpretation of the invention and emphasizes the open interpretation of terms and numerical ranges. It points out that the terms mentioned in the invention should be understood to describe specific embodiments rather than to limit the scope of the invention. In addition, numerical ranges should be understood to include every intervening value within the range, as well as the upper and lower limits of any smaller ranges, which may independently be included or excluded in the range.

[0027] Emphasis is also placed on the general understanding of technical and scientific terms and on the indication that although preferred methods and materials are provided, similar or equivalent methods and materials can also be used in the practice or testing.

[0028] Additionally, references to cited literature are included herein to indicate that they are incorporated by reference to disclose and describe relevant methods and materials, and in the event of any conflict with the literature, the present specification shall prevail.

[0029] Finally, it is clearly pointed out that modifications and variations can be made to the specific embodiments without departing from the scope or spirit of the invention, which will be obvious to those skilled in the art. At the same time, this description and examples are only for illustrative purposes.

[0030] The present invention will be further described below with reference to the embodiments.

[0031] The present invention provides a composite hydrogel for pet wound care, the raw materials of which include lignin-copper gluconate complex, sodium hyaluronate, propylene glycol, and aloe extract, wherein the amount of copper ions added to the lignin-copper gluconate complex is 0.01% to 0.1% by mass;

[0032] Calculated by mass percentage, the raw materials include: 0.01% to 10% of sodium hyaluronate, 0.1% to 10% of propylene glycol, and 0.01% to 5% of aloe vera extract are added to every 100 mL of lignin-copper gluconate complex, and the pH value is adjusted to a range of 5.8 to 7.2 to obtain a complex hydrogel for pet wound care.

[0033] The preparation method of the composite hydrogel for pet wound care comprises the following steps:

[0034] S1. Dissolve 4 g of lignin in 100 mL of purified water and stir evenly to obtain a lignin solution.

[0035] S2. Dissolve 0.2 g of copper gluconate in 100 mL of purified water and stir evenly to obtain a copper gluconate solution.

[0036] S3. Mix the lignin solution and the copper gluconate solution in a volume ratio of 1:1, stir evenly, and react at room temperature for 2 hours to form a lignin-copper gluconate complex.

[0037] S4. Adjust the amount of copper ions added to the lignin-copper gluconate complex. Add sodium hyaluronate, propylene glycol, and aloe extract to 100 mL of the lignin-copper gluconate complex in sequence and stir evenly.

[0038] S5. Continue the reaction at room temperature for 1 hour to obtain lignin-copper gluconate hydrogel.

[0039] S6. Finally, the S5 is ultrasonically treated, the pH value is adjusted, and the mixture is bottled to prepare a composite hydrogel for pet wound care.

[0040] Furthermore, in step 3, the stirring time is 30 min to 90 min, and the stirring condition is 600 r / min.

[0041] Furthermore, in step 4, the stirring time is 30 min to 90 min, and the stirring condition is 1000 r / min.

[0042] Furthermore, the purpose of the ultrasonic treatment in step 6 is to remove bubbles in the hydrogel, the ultrasonic time is 15 minutes to 30 minutes, and the ultrasonic treatment process conditions are an ultrasonic power of 100 W and an ultrasonic frequency of 25 kHz.

[0043] Example 1

[0044] A composite hydrogel for pet wound care, composed of the following raw materials:

[0045] Lignin-copper gluconate complex, sodium hyaluronate 0.5%, propylene glycol 1%, aloe vera extract 0.5%.

[0046] A method for preparing a composite hydrogel for pet wound care, comprising the following steps:

[0047] S1. Dissolve lignin in purified water and stir evenly to obtain a lignin solution.

[0048] S2. Dissolve copper gluconate in purified water and stir evenly to obtain a copper gluconate solution.

[0049] S3. Mix the lignin solution and the copper gluconate solution in a volume ratio of 1:1 and stir evenly. React at room temperature for 2 hours to form a lignin-copper gluconate complex. Stir for 90 minutes at 600 rpm.

[0050] S4. Adjust the copper ion addition amount in the lignin-copper gluconate complex to 0.01%. Add sodium hyaluronate, propylene glycol and aloe extract to 100 mL of the lignin-copper gluconate complex in sequence and stir evenly.

[0051] S5. Continue the reaction at room temperature for 1 hour to obtain lignin-copper gluconate hydrogel.

[0052] S6. Finally, ultrasonically treat S4 to remove bubbles in the hydrogel. The ultrasonic treatment time is 30 minutes, the ultrasonic power is 100 W, and the ultrasonic frequency is 25 kHz. Cool the tube, seal it with plastic wrap, and place it in a cool cabinet at 4°C to obtain the composite hydrogel for pet wound care.

[0053] Example 2

[0054] A composite hydrogel for pet wound care, composed of the following raw materials:

[0055] Lignin-copper gluconate complex, sodium hyaluronate 0.8%, propylene glycol 2%, aloe vera extract 1%.

[0056] A method for preparing a composite hydrogel for pet wound care, comprising the same steps as in Example 1, except that in S4 the amount of copper ions added to the lignin-copper gluconate composite is adjusted to 0.07%;

[0057] Example 3

[0058] A composite hydrogel for pet wound care, composed of the following raw materials:

[0059] Lignin-copper gluconate complex, sodium hyaluronate 1%, propylene glycol 2.5%, aloe vera extract 2%.

[0060] A method for preparing a composite hydrogel for pet wound care, comprising the same steps as in Example 1, except that the amount of copper ions added to the lignin-copper gluconate composite is adjusted to 0.01%;

[0061] Example 4

[0062] A composite hydrogel for pet wound care, composed of the following raw materials:

[0063] Lignin-copper gluconate complex, sodium hyaluronate 0.8%, propylene glycol 4%, aloe vera extract 3%.

[0064] A method for preparing a composite hydrogel for pet wound care, comprising the same steps as in Example 1, except that the amount of copper ions added to the lignin-copper gluconate composite is adjusted to 0.1%;

[0065] Example 5

[0066] A composite hydrogel for pet wound care, composed of the following raw materials:

[0067] Lignin-copper gluconate complex, sodium hyaluronate 0.01%, propylene glycol 2%, aloe vera extract 4%.

[0068] A method for preparing a composite hydrogel for pet wound care, comprising the same steps as in Example 1, except that the amount of copper ions added to the lignin-copper gluconate composite is adjusted to 0.05%;

[0069] Example 6

[0070] A composite hydrogel for pet wound care, composed of the following raw materials:

[0071] Lignin-copper gluconate complex, sodium hyaluronate 10%, propylene glycol 5%, aloe vera extract 2%.

[0072] A method for preparing a composite hydrogel for pet wound care, comprising the same steps as in Example 1, except that the amount of copper ions added to the lignin-copper gluconate composite is adjusted to 0.02%;

[0073] Example 7

[0074] A composite hydrogel for pet wound care, composed of the following raw materials:

[0075] Lignin-copper gluconate complex, sodium hyaluronate 1.5%, propylene glycol 0.1%, aloe vera extract 3.5%.

[0076] A method for preparing a composite hydrogel for pet wound care, comprising the same steps as in Example 1, except that the amount of copper ions added to the lignin-copper gluconate composite is adjusted to 0.08%;

[0077] Example 8

[0078] A composite hydrogel for pet wound care, composed of the following raw materials:

[0079] Lignin-copper gluconate complex, sodium hyaluronate 3%, propylene glycol 10%, aloe vera extract 4%.

[0080] A method for preparing a composite hydrogel for pet wound care, comprising the same steps as in Example 1, except that the amount of copper ions added to the lignin-copper gluconate composite is adjusted to 0.03%;

[0081] Example 9

[0082] A composite hydrogel for pet wound care, composed of the following raw materials:

[0083] Lignin-copper gluconate complex, sodium hyaluronate 3%, propylene glycol 4%, aloe extract 0.01%.

[0084] A method for preparing a composite hydrogel for pet wound care, comprising the same steps as in Example 1, except that the amount of copper ions added to the lignin-copper gluconate composite is adjusted to 0.05%;

[0085] Example 10

[0086] A composite hydrogel for pet wound care, composed of the following raw materials:

[0087] Lignin-copper gluconate complex, sodium hyaluronate 3.5%, propylene glycol 5%, aloe vera extract 5%.

[0088] A method for preparing a composite hydrogel for pet wound care comprises the same steps as in Example 1, except that the amount of copper ions added to the lignin-copper gluconate composite is adjusted to 0.06%.

[0089] Effect Example 1

[0090] (1) The hydrogel prepared in Example 4 was characterized. The results are shown in Figure 1 .

[0091] Figure 1 This is a schematic diagram of the appearance of the gel prepared in Example 4 of the present invention;

[0092] from Figure 1 It can be seen that the gel prepared in Example 4 of the present invention is a light blue gel.

[0093] (2) Antibacterial activity detection

[0094] In order to explore the bactericidal effect of the composite hydrogel on Candida albicans, the following experimental steps were performed:

[0095] First, the composite hydrogel was prepared according to Examples 1 to 4. At the same time, a group of hydrogels without the addition of the lignin-copper gluconate composite was prepared according to Example 1 as a blank control group of the experiment.

[0096] Next, prepare a Candida albicans seed solution with a concentration of 2×10 6 Then, the same amount of Candida albicans seed solution was added to each composite hydrogel and the blank control group.

[0097] Subsequently, the mixed solutions were placed in an environment of 30°C and subjected to drug treatment at a rotation speed of 200 rpm for 3 hours.

[0098] Finally, the colony dilution plate counting method was used to count the treated Candida albicans to further analyze the bactericidal effect of the composite hydrogel on Candida albicans.

[0099] Figure 2 It is a drop plate diagram and survival rate statistical diagram of an embodiment of the present invention.

[0100] from Figure 2 It can be seen from the results that the examples of the present invention have a significant bactericidal effect on Candida albicans, indicating that the composite hydrogel for pet wound care has good anti-Candida albicans activity.

[0101] (III) Construction of a wound infection mouse model and evaluation of the effects of the composite hydrogel

[0102] Seven-week-old SPF-grade ICR male experimental mice were acclimated and divided into five groups for skin wound modeling. After anesthesia, the mice were anesthetized and their back hair was removed to fully expose the skin. After thoroughly cleaning the exposed skin with iodine, a circular, full-thickness wound approximately 10 mm in diameter was created on the back of the mice.

[0103] Disinfect the wound and surrounding skin with iodine. The day of modeling is designated Day 1. Prepare the composite hydrogel according to Examples 1-4. Simultaneously, prepare a group of hydrogels without the addition of the lignin-copper gluconate complex as described in Example 1 as a blank control group. Starting on Day 1, evenly cover the wound surface with 50 μL of the hydrogel once daily.

[0104] After wound incision, the wound surface was photographed daily. A steel ruler was placed next to the wound of the experimental mouse to photograph the wound surface. All wounds were magnified equally using the steel ruler as a standard, and the wound area of ​​the mice was quantified using Image J image analysis software.

[0105] Figure 3 This is a graph showing the changes in wound area in mice according to an embodiment of the present invention.

[0106] from Figure 3 It can be seen from the figure that the embodiments of the present invention have a significant effect on wound healing. This shows that the composite hydrogel for pet wound care can promote wound healing.

[0107] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. Application of lignin copper ion complex hydrogel in the preparation of a drug for treating fungal infection of pet wounds caused by Candida albicans, characterized in that: The raw materials of the lignin-copper ion complex hydrogel include: lignin-copper gluconate complex, sodium hyaluronate, propylene glycol, and aloe extract, wherein the amount of copper ions added to the lignin-copper gluconate complex is 0.1% by mass; In terms of mass percentage, 0.8% sodium hyaluronate, 4% propylene glycol, and 3% aloe vera extract were added to every 100 mL of the lignin-copper gluconate complex, and the pH value was adjusted to a range of 5.8 to 7.2 to obtain a complex hydrogel for pet wound care. When preparing the lignin-copper gluconate complex, equal volumes of lignin solution and copper gluconate solution are mixed, and the lignin-copper gluconate complex is obtained after reaction; The preparation steps of the above-mentioned lignin copper ion complex hydrogel are specifically as follows: S1. Take 100 mL of lignin-copper gluconate complex. The copper ion addition amount in the lignin-copper gluconate complex is 0.1% by mass. S2. Sodium hyaluronate, propylene glycol and aloe extract are sequentially added to the lignin-copper gluconate complex, and the pH value is adjusted to be neutral or weakly acidic to obtain a lignin-copper ion complex hydrogel.

2. The use according to claim 1, characterized in that The fungal infection is an epidermal fungal infection.

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