A high-efficiency liquid suspension culture method for pinellia ternata

CN118947544BActive Publication Date: 2026-06-26HUBEI UNIV OF CHINESE MEDICINE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HUBEI UNIV OF CHINESE MEDICINE
Filing Date
2024-09-04
Publication Date
2026-06-26

AI Technical Summary

Technical Problem

Existing methods for propagating Pinellia ternata are inefficient, have low propagation coefficients, and involve long seedling production cycles in industrialized production. Liquid culture seedling production also has an excessively long cycle and requires considerable manpower for cleaning the roots, hindering the development of Pinellia ternata production and genetic transformation system research.

Method used

Using Pinellia ternata leaves or petioles as explants, embryogenic callus was induced through liquid suspension culture technology to establish a stable somatic embryogenesis system, directly obtaining granular proembryo tissue, and achieving rapid plant regeneration by optimizing culture medium and conditions.

Benefits of technology

The embryogenic callus induction rate of Pinellia ternata reached as high as 81.6%, the regeneration cycle was shortened to about 4 weeks, the seedlings were ready for planting, the propagation coefficient was improved, the operation steps were simplified, and the cost was reduced, laying the foundation for the industrial production of high-quality Pinellia ternata seedlings.

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Abstract

The application belongs to the field of in vitro rapid propagation of Chinese medicine seedling, and particularly relates to a high-efficiency liquid suspension culture method of pinellia ternate, which comprises obtaining of pinellia ternate explants under sterile conditions, induction of embryogenic callus, liquid suspension culture, somatic embryo development and maturation, somatic embryo germination and transplanting of regenerated seedlings. By using the method, the induction rate of pinellia ternate embryogenic callus can reach 81.6%, stable mature cotyledon embryos can be obtained, and the embryos can be regenerated into complete plants, a high-efficiency and stable regeneration system is successfully established, favorable conditions are created for industrial production of pinellia ternate fine seedlings, and a good foundation is laid for genetic transformation of pinellia ternate.
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