Insecticide composition and its application

Abstract

The present invention belongs to the field of pesticide insecticide technology and discloses an insecticidal composition and its use. The active ingredients of the insecticidal composition include active ingredient A and active ingredient B. Active ingredient A is a compound of formula I: #imgabs0#, and active ingredient B is any one of flupyroxystrobin, indazapyroxamet, and nicofluprole. The mass ratio of active ingredient A to active ingredient B is 1:35 to 30:1. The insecticidal composition of the present invention has a significant synergistic effect on Lepidoptera and Coleoptera pests, exhibits significant control efficacy, significantly reduces the degree of damage caused by pests, slows the development of pesticide resistance in pests, and facilitates integrated pest control.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide killing technology and discloses an insecticide composition and application thereof. Background Art

[0002] Flupyroxystrobin is a methoxyacrylate insecticide with the CAS registration number 114077-75-7 and the chemical name: (2E)-3-methoxy-2-(2-{[4-(trifluoromethyl)pyridin-2-yl]oxy}phenyl)prop-2-enoic acid methyl ester. It has high activity against Diptera pests such as Melon Fly, Olive Fruit Fly, Rapeseed Midge, Wheat Field Fly, Gray Field Fly, Cabbage Field Fly, Vegetable Leaf Miner, American Leaf Miner, Onion Leaf Miner, as well as Thysanoptera pests such as Western Flower Thrips, Rice Thrips, Onion Thrips, Melon Thrips, and Root Maggots. Its structural formula is shown below:

[0003]

[0004] Indazapyroxamet is an indoleamide insecticide containing pyridyl and cyclopropyl groups. Its CAS number is 1689545-27-4 and its chemical name is N-(1-methylcyclopropyl)-2-(3-pyridyl)-2H-benzopyrazole-4-carboxamide. It has good activity against pests such as whiteflies, thrips, leafhoppers, and cotton aphids. Its structural formula is shown below:

[0005]

[0006] Nicofluprole is a new fluorinated insecticide from the phenylpyrazolamide class. Its CAS number is 1771741-86-6, and its chemical name is 2-chloro-N-cyclopropyl-5-(1-(2,6-dichloro-4-(perfluoropropane-2-yl)phenyl)-1H-pyrazol-4-yl)-N-methylnicotinamide. It has excellent control effects against pests such as aphids, spider mites, diamondback moths, and fall armyworms. Its structural formula is shown below:

[0007]

[0008] In pest control, the use of mixed insecticides has been promoted and applied to increase prevention and achieve dual treatment. Rationalized compounding of insecticides can enhance control effectiveness, delay the development of resistance in pests and diseases, improve control effectiveness, and reduce dosage. Compounding insecticides for different pests and diseases can also reduce the number of applications, thereby lowering labor costs. Compounding insecticides is not simply a matter of mixing; rather, it requires scientific and rational compounding based on concentration, application method, and efficacy. Summary of the Invention

[0009] In order to solve the above-mentioned problems existing in the prior art, the present invention provides an insecticidal combination, which compounds insecticidal compounds with different mechanisms of action according to a reasonable mass ratio, has a significant synergistic effect on agricultural, horticultural and forestry Diptera and Hemiptera pests, has a significant control effect, effectively reduces the amount of pesticide used, and slows down the development of pest resistance.

[0010] Another object of the present invention is to provide a pesticide formulation comprising a compound of formula I and an insecticidal composition selected from the group consisting of flupyroxystrobin, indazapyroxamet, and nicofluprole. The formulation has reasonable composition, can be used to control a variety of pests, is safe for target crops, and is beneficial to integrated pest control.

[0011] In order to achieve the above object, the present invention adopts the following technical solution: an insecticide composition, wherein the active ingredients of the insecticide composition include active ingredient A and active ingredient B, and the active ingredient A is a compound of formula I: The active ingredient B is any one of flupyroxystrobin, indazapyroxamet, and nicofluprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 30:1, or any value within the above numerical range.

[0012] Furthermore, the active ingredient B is flupyroxystrobin, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 25:1, or any value within the above numerical range;

[0013] The active ingredient B is indazapyroxamet, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 18:1, or any value within the above numerical range;

[0014] The active ingredient B is nicofluprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 25:1, or any value within the above numerical range.

[0015] Furthermore, the active ingredient B is flupyroxystrobin, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 16:1, or any value within the above numerical range;

[0016] The active ingredient B is indazapyroxamet, and the mass ratio of the active ingredient A to the active ingredient B is 1:10 to 12:1, or any value within the above numerical range;

[0017] The active ingredient B is nicofluprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:15 to 20:1, or any value within the above numerical range.

[0018] Furthermore, the active ingredient B is flupyroxystrobin, and the mass ratio of the active ingredient A to the active ingredient B is 1:10 to 12:1, or any value within the above numerical range;

[0019] The active ingredient B is indazapyroxamet, and the mass ratio of the active ingredient A to the active ingredient B is 1:5 to 8:1, or any value within the above numerical range;

[0020] The active ingredient B is nicofluprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:10 to 15:1, or any value within the above numerical range.

[0021] Furthermore, the total weight of the insecticide composition is calculated as 100 wt%, and the active ingredient A and active ingredient B account for 1% to 70% of the total weight of the insecticide composition;

[0022] Furthermore, the insecticide composition contains auxiliary ingredients in addition to the active ingredients, and the auxiliary ingredients are one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, preservatives, stabilizers, synergists or carriers.

[0023] The wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium lauryl sulfate, sodium dioctyl sulfosuccinate, α-olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, soapberry powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0024] The dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0025] The emulsifier is selected from one or more of calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0026] The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0027] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0028] The antifreeze agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0029] Defoaming agent selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of fatty alcohols; and / or

[0030] The solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel, solvent oil, vegetable oil, vegetable oil derivatives and water; and / or

[0031] The preservative is selected from one or more of propionic acid, sodium propionic acid, sorbic acid, sodium sorbic acid, potassium sorbic acid, benzoic acid, sodium benzoic acid, sodium p-hydroxybenzoic acid, methyl p-hydroxybenzoate, kasone and 1,2-benzisothiazolin-3-one; and / or

[0032] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc, montmorillonite and starch; and / or

[0033] Synergists are selected from synergist, piperonyl butoxide; and / or

[0034] The carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives;

[0035] Furthermore, the insecticide composition is prepared into a formulation permitted by pesticides, and the formulation is a solid formulation or a liquid formulation;

[0036] Furthermore, the solid preparation includes powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules;

[0037] The liquid preparations include soluble solutions, soluble gels, oils, film-spreading oils, emulsifiable concentrates, latexes, dispersible solutions, ointments, aqueous emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspoemulsions, microcapsule suspension-suspension concentrates, microcapsule suspension-water emulsions, or microcapsule suspension-suspoemulsions;

[0038] Furthermore, the solid preparation is a water-dispersible granule or a wettable powder, and the liquid preparation is a suspension.

[0039] The present invention also discloses the use of the insecticide composition for preventing and controlling agricultural, horticultural and forestry pests.

[0040] Furthermore, the pests are Diptera pests or Hemiptera pests;

[0041] In particular, the insecticide composition of the present invention has an obvious synergistic effect on the Diptera pest leafminer and the Hemiptera pest aphid, and has a significant control effect.

[0042] In order to obtain the desired insecticidal effect, the dosage of the pesticide composition varies depending on various factors, such as the crop to be protected, the type of pest, the degree of infection, climatic conditions, application site, application method, dosage form used, etc.

[0043] The beneficial effects of the present invention are as follows:

[0044] 1) The present invention rationally compounds insecticidal compounds with different mechanisms of action, with reasonable components, and has obvious synergistic effects on various pests in agriculture, horticulture, and forestry, with significant control effects;

[0045] 2) The insecticide composition of the present invention is safe to target crops and non-target organisms, meets the requirements of integrated pest control with safe pesticides, reduces the amount of pesticides used, lowers production costs, and alleviates environmental pressure. DETAILED DESCRIPTION

[0046] In order to make the technical solutions, objectives and advantages of the present invention more clearly understood, the present invention is described in conjunction with the following specific embodiments. However, the present invention can be implemented in various forms and should not be limited to the embodiments described herein.

[0047] Preparation example:

[0048] Preparation Example 1: 25% Formula I compound flupyroxystrobin suspension (3:2)

[0049] Formula composition: 15% compound of formula I, 10% flupyroxystrobin, 1% sodium lauryl sulfate, 2% succinate sulfonate, 2% polyoxyethylene sorbitan monooleate, 3% fatty alcohol polyoxyethylene ether phosphate, 0.25% xanthan gum, 1% magnesium aluminum silicate, 4% propylene glycol, 0.01% potassium benzoate, 0.5% silicone oil, and deionized water to make up the balance;

[0050] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, water is added and mixed evenly, and the suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0051] Preparation Example 2: 30% Formula I compound flupyroxystrobin water dispersible granules (4:1)

[0052] Formula composition: 24% compound of formula I, 6% flupyroxystrobin, 12% naphthalenesulfonate formaldehyde condensate, 4% polycarboxylate sodium salt, 2% sodium lauryl sulfate, 5% white carbon black, 25% starch, and kaolin makes up the balance;

[0053] Preparation method: According to the formula ratio of the embodiment, the active ingredient is added to the carrier, and a surfactant and other functional additives are added thereto, mixed, and after air flow grinding, 10-25% water is added, and then kneading, granulation, drying, and sieving are carried out to obtain a water-dispersible granule product; or the pulverized powder is sprayed with water in a boiling granulator, granulated, dried, and then sieved to obtain the product.

[0054] Preparation Example 3: 24% Formula I compound flupyroxystrobin wettable powder (1:5)

[0055] Formula composition: 4% compound of formula I, 20% flupyroxystrobin, 4% sodium lignin sulfonate, 5% fatty alcohol polyoxyethylene ether sulfate, 3% opening powder BX, 5% white carbon black, and kaolin to make up the balance;

[0056] Preparation method: The active ingredients, dispersant, wetting agent and filler are mixed according to the formula ratio, uniformly stirred in a stirring kettle, and pulverized and mixed uniformly for multiple times in a jet mill to prepare the wettable powder of the composition of the present invention.

[0057] Preparation Example 4: 15% Formula I compound·indazapyroxamet suspension (1:1)

[0058] Formula composition: 7.5% compound of formula I, 7.5% indazapyroxamet, 3% triphenol polyoxyethylene ether phosphate, 5% Guerbet alcohol polyoxyethylene ether, 1% sodium lignin sulfonate, 1% magnesium aluminum silicate, 0.25% xanthan gum, 5% glycerol, 0.1% sodium benzoate, 0.5% silicone oil, and deionized water to make up the balance;

[0059] Preparation method: Same as Preparation Example 1.

[0060] Preparation Example 5: 32% Formula I compound·indazapyroxamet water dispersible granules (3:1)

[0061] Formula composition: 24% compound of formula I, 8% indazapyroxamet, 6% sodium alkyl polyoxyethylene ether sulfonate, 3% sodium alkyl naphthalene sulfonate, 2% sodium lauryl sulfate, 8% ammonium sulfate, and starch makes up the balance;

[0062] Preparation method: same as Preparation Example 2.

[0063] Preparation Example 6: 27% Formula I compound·indazapyroxamet wettable powder (8:1)

[0064] Formula composition: 24% compound of formula I, 3% indazapyroxamet, 2% sodium lauryl sulfate, 5% alkylnaphthalene sulfonate, 6% polynaphthaldehyde sulfonic acid sodium salt, 10% kaolin, 10% white carbon black, and bentonite to make up the balance;

[0065] Preparation method: Same as Preparation Example 3.

[0066] Preparation Example 7: 18% Formula I compound·nicofluprole suspension (1:1)

[0067] Formula composition: 9% compound of formula I, 9% nicofluprole, 3% phenethylphenol polyoxyethylene polyoxypropylene ether phosphate, 3% alkylaryl polyoxyethylene ether polyoxypropylene ether, 2% polyoxyethylene sorbitan monooleate, 2% sodium polycarboxylate, 1.5% magnesium aluminum silicate, 0.1% carboxyethyl cellulose, 1% sodium sorbate, 5% ethylene glycol, 0.5% silicone oil, and deionized water to make up the balance;

[0068] Preparation method: Same as Preparation Example 1.

[0069] Preparation Example 8: 36% Formula I compound·nicofluprole water dispersible granules (1:5)

[0070] Formula composition: 6% compound of formula I, 30% nicofluprole, 10% lignin sulfonate, 5% sodium fatty alcohol polyoxyethylene ether sulfate, 2% sodium lauryl sulfate, 5% white carbon black, 30% starch, and kaolin makes up the balance;

[0071] Preparation method: same as Preparation Example 2.

[0072] Preparation Example 9: 22% Formula I compound·nicofluprole wettable powder (10:1)

[0073] Formula composition: 20% compound of formula I, 2% nicofluprole, 3% sodium salt of polycarboxylate, 3% sodium dioctyl sulfosuccinate, 2% flaking powder BX, 8% methylnaphthalene sulfonate formaldehyde condensate, 5% white carbon black, and kaolin to make up the balance;

[0074] Preparation method: Same as Preparation Example 3.

[0075] Example 1: Indoor bioactivity assay of Liriomyza sativae

[0076] The test insect source: Liriomyza sativae, mainly 1st to 2nd instar.

[0077] Test insect management: Field-collected bean leaves with larvae were placed in a 20 cm long, 30 cm wide porcelain tray in an insectary at a temperature of (25 ± 1) ° C, a humidity of (80 ± 10)%, and a photoperiod of L:D = 14 h:10 h. After pupation, the pupae were collected in a wide-mouthed bottle, covered with a mesh stopper, and fed with 8% honey water for one day after emergence. Two 20-day-old bean seedlings (with the growing point removed and only two primary leaves remaining, the same below) per tray (15 cm in diameter and 15 cm in height) were exposed to the newly emerged adults for 2 hours and then moved to an artificial climate chamber (temperature 26 ° C ± 1 ° C, humidity 84% ± 10%, photoperiod L:D = 14:10 h) for daily observation of their development progress.

[0078] Test agents: compound of formula I, Flupyroxystrobin original drug.

[0079] Preparation of the drug: Dissolve the original drug in a suitable solvent to prepare a high-concentration mother solution, and then dilute the mother solution with a 0.1% Tween-80 aqueous solution. Set 5 different concentrations for each treatment and set aside.

[0080] Chemical treatment: When tunnels longer than 0.2 cm appear on leaves, select cowpea leaves with tunnels and larvae. Dip the leaves in the chemical solution for 10 seconds. Wrap the base of the leaves with wet cotton balls, let them dry slightly, and then place them in a covered plastic box. Each treatment consists of 20 test insects. After treatment, the insects were housed in a constant temperature incubator at (25 ± 1)°C. Each treatment was replicated four times, and a treatment without chemical (containing solvents and emulsifiers) served as a blank control.

[0081] Investigation: 48 hours after treatment, the number of dead and live worms in each treatment was examined under a binocular dissecting microscope, and the mortality rate and adjusted mortality rate were calculated.

[0082] Data statistics and analysis:

[0083] Based on the survey data, calculate the adjusted mortality rate of each treatment using formulas (1) and (2), with the results rounded to two decimal places:

[0084]

[0085] Where:

[0086] P——mortality rate, in percentage (%);

[0087] K——number of dead insects, in heads;

[0088] N——Total number of insects processed, in heads.

[0089]

[0090] Where:

[0091] P1——adjusted mortality rate, in percentage (%);

[0092] P t ——Treatment mortality rate, in percentage (%);

[0093] P0 - blank control mortality rate, in percentage (%).

[0094] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to formula (2); if the control mortality rate is greater than 20%, the test needs to be repeated.

[0095] Use DPS statistical analysis system to analyze and find the toxicity regression line and LC 50 The activity of the test agent on the biological test material was evaluated by using the value and correlation coefficient R.

[0096] The co-toxicity coefficient (CTC value) of the mixture is calculated according to formula (3), formula (4), and formula (5):

[0097]

[0098] Where:

[0099] ATI - measured toxicity index of mixture;

[0100] S——LC of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0101] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0102] TTI=TI A ×P A +TI B ×P B ···········(4)

[0103] Where:

[0104] TTI – Theoretical Toxicity Index of Mixtures;

[0105] TI A ——Agent toxicity index;

[0106] P A ——The percentage of agent A in the mixture, in percentage (%);

[0107] TI B ——Toxicity index of agent B;

[0108] P B ——The percentage of agent B in the mixture, in percentage (%).

[0109]

[0110] Where:

[0111] CTC – Co-toxicity coefficient;

[0112] ATI - measured toxicity index of mixture;

[0113] TTI - Theoretical Toxicity Index of Mixture.

[0114] A co-toxicity coefficient (CTC) of 120 or higher indicates a synergistic effect; a co-toxicity coefficient (CTC) of 80 or lower indicates an antagonistic effect; and a co-toxicity coefficient (CTC) of 80 or lower indicates an additive effect.

[0115] Table 1 Results of indoor biological activity test of compound I combined with Flupyroxystrobin against Liriomyza sativae leafminer

[0116]

[0117] Laboratory test results (see Table 1) show that the compound of Formula I and Flupyroxystrobin exhibit a synergistic effect against Liriomyza sativae at appropriate ratios. When the mass ratio of the compound of Formula I to Flupyroxystrobin was 1:30 to 25:1, the co-toxicity coefficient against Liriomyza sativae was greater than 120, demonstrating a synergistic effect. When the mass ratio of the compound of Formula I to Flupyroxystrobin was 1:20 to 16:1, the co-toxicity coefficient was greater than 130, indicating a significant synergistic effect. When the mass ratio of the compound of Formula I to Flupyroxystrobin was 1:10 to 12:1, the co-toxicity coefficient was greater than 150, indicating a significant synergistic effect. When the mass ratio of the compound of Formula I to Flupyroxystrobin was 3:2, the co-toxicity coefficient against Liriomyza sativae was the highest, reaching 204.912.

[0118] Example 2: Indoor bioactivity assay of alfalfa aphid

[0119] Test target: Alfalfa aphid (Aphis medicaginis Koch).

[0120] Test agents: Compound of formula I, Indazapyroxamet, and Nicofluprole original drug.

[0121] Preparation of pharmaceuticals: Prepare the mother liquor of single doses separately, and design a reasonable ratio according to the mixing purpose and the activity of the pharmaceuticals. Each single dose and each group of mixed doses shall be prepared into the required series of mass concentrations according to the equal ratio method.

[0122] Assay Method: Untreated cabbage leaves of similar size and color were washed and dried, then discs were prepared. These discs were immersed in various concentrations of the drug solution for 5 seconds, removed, dried, and placed in Petri dishes lined with moisturizing filter paper. Twenty wingless adult aphids of similar size and vigor were selected and attached to the discs treated with each drug solution. Each treatment and control were repeated four times. After treatment, the Petri dishes were placed in a climate chamber at (23±1)°C, relative humidity (60±10)%, and a light intensity ratio of 16h:8h. Microscopic examination was performed after 48 hours.

[0123] Test investigation: Use a brush to gently touch the insect body. If there is no spontaneous reaction, it is considered dead. The number of dead insects is recorded.

[0124] Data statistics and analysis:

[0125] Based on the survey data, calculate the adjusted mortality rate of each treatment using formulas (1) and (2), with the results rounded to two decimal places:

[0126]

[0127] Where:

[0128] P——mortality rate, in percentage (%);

[0129] K——number of dead insects, in heads;

[0130] N——Total number of insects processed, in heads.

[0131]

[0132] Where:

[0133] P1——adjusted mortality rate, in percentage (%);

[0134] P t ——Treatment mortality rate, in percentage (%);

[0135] P0 - blank control mortality rate, in percentage (%).

[0136] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to formula (2); if the control mortality rate is greater than 20%, the test needs to be repeated.

[0137] Use DPS statistical analysis system to analyze and find the toxicity regression line and LC 50 The activity of the test agent on the biological test material was evaluated by using the value and correlation coefficient R.

[0138] The co-toxicity coefficient (CTC value) of the mixture is calculated according to formula (3), formula (4), and formula (5):

[0139]

[0140] Where:

[0141] ATI - measured toxicity index of mixture;

[0142] S——LC of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0143] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0144] TTI=TI A ×P A +TI B ×P B ···········(4)

[0145] Where:

[0146] TTI – Theoretical Toxicity Index of Mixtures;

[0147] TIA ——Agent toxicity index;

[0148] P A ——The percentage of agent A in the mixture, in percentage (%);

[0149] TI B ——Toxicity index of agent B;

[0150] P B ——The percentage of agent B in the mixture, in percentage (%).

[0151]

[0152] Where:

[0153] CTC – Co-toxicity coefficient;

[0154] ATI - measured toxicity index of mixture;

[0155] TTI - Theoretical Toxicity Index of Mixture.

[0156] A co-toxicity coefficient (CTC) of 120 or higher indicates a synergistic effect; a co-toxicity coefficient (CTC) of 80 or lower indicates an antagonistic effect; and a co-toxicity coefficient (CTC) of 80 or lower indicates an additive effect.

[0157] Table 2 Results of indoor bioactivity test on alfalfa aphids by combining compound of formula I and indazapyroxamet

[0158]

[0159] The results of the indoor test show that (see Table 2) the compound of formula I and indazapyroxamet exhibit a synergistic effect on alfalfa aphids when mixed in an appropriate ratio. The mass ratio of the compound of formula I to indazapyroxamet is 1:20 to 18:1, and the co-toxicity coefficient to alfalfa aphids is greater than 120, showing a synergistic effect; the mass ratio of the compound of formula I to indazapyroxamet is 1:10 to 12:1, and the co-toxicity coefficient is greater than 130, showing a significant synergistic effect; the mass ratio of the compound of formula I to indazapyroxamet is 1:5 to 8:1, and the co-toxicity coefficient is greater than 150, showing a significant synergistic effect; the compound of formula I and indazapyroxamet is 1:5 to 8:1, and the co-toxicity coefficient is greater than 150, showing a significant synergistic effect.

[0160] The mass ratio of indazapyroxamet was 3:1, and the co-toxicity coefficient to alfalfa aphid was the largest, which was 182.650.

[0161] Table 3 Results of indoor bioactivity test on alfalfa aphids by combining compound of formula I and Nicofluprole

[0162]

[0163]

[0164] The results of the indoor test show that (see Table 3) the compound of formula I and Nicofluprole exhibit a synergistic effect on alfalfa aphids when mixed in an appropriate ratio. The mass ratio of the compound of formula I to Nicofluprole is 1:20 to 25:1, and the co-toxicity coefficient to alfalfa aphids is greater than 120, showing a synergistic effect; the mass ratio of the compound of formula I to Nicofluprole is 1:15 to 20:1, and the co-toxicity coefficient is greater than 130, showing a significant synergistic effect; the compound of formula I and Nicofluprole have a synergistic effect.

[0165] The mass ratio of Nicofluprole is 1:10-15:1, the co-toxicity coefficient is greater than 150, and the synergistic effect is significant; the mass ratio of Formula I compound to Nicofluprole is 1:1, and the co-toxicity coefficient to alfalfa aphid is the largest, which is 192.303.

[0166] Example 3: Field efficacy test for controlling leafminers

[0167] Test basis: The test refers to GB / T 17980.66-2004 "Guidelines for Field Efficacy Tests of Pesticides (II) Part 66: Insecticides for Control of Vegetable Leaf Miners".

[0168] The test was conducted in a greenhouse in Qingguang Village, Qingguang Town, Beichen District, Tianjin. The soil fertility was moderate, the terrain flat, and the soil was sandy loam. Crop varieties, planting dates, growth conditions, and fertilizer and water management were largely consistent. During the control period, the leafminer was in its larval stage.

[0169] Test target: Liriomyza sativae leafminer.

[0170] Experimental crops: cucumber.

[0171] Experimental plot arrangement: The experimental plot area is 15m 2 Each treatment was repeated 4 times, and all experimental plots were arranged in random blocks.

[0172] Test Method: A Linong 16L (HD-400) backpack sprayer was used for one conventional application. Ten cucumber plants were surveyed in the middle row of each plot. Two infested leaves, located in the middle and upper parts of each plant, were marked. Three to five early-stage larval tunnels (0.5 to 1 cm long, representing larval stages of approximately 1 to 2 years) were required. Approximately 1 cm from the front of each tunnel, a dot was marked on either side with a marker, aligned with the front of the tunnel. A baseline count was performed before application, and 3 and 14 days after application, recording the number of empty tunnels, dead insects, and live insects.

[0173] The standard for determining whether the larvae in the tunnel are dead or alive is as follows: larvae with fresh, plump body color and with pupation holes are alive. If it is difficult to identify, those with extended worm tunnels, pupation holes and new tunnels are alive; those with shriveled, discolored bodies and no extended worm tunnels are dead.

[0174] Calculation method of drug efficacy:

[0175]

[0176] Table 4 Results of field trials on the efficacy of Liriomyza sativae

[0177]

[0178]

[0179] The results of the field efficacy test show that (see Table 4), the two compound pesticides of 25% Formula I compound·flupyroxystrobin suspension concentrate (3:2) and 24% Formula I compound·flupyroxystrobin wettable powder (1:5) have significant control effects on leafminers.

[0180] The control effect of 25% formula I compound·flupyroxystrobin suspension (3:2) and 24% formula I compound·flupyroxystrobin wettable powder (1:5) at an active ingredient dosage of 8g / hm2 was observed 3 days after application. 2 Under the conditions of 25% formula I compound and flupyroxystrobin suspension concentrate (3:2), the highest control efficacy was 93.81% at 14 days after application.

[0181] Example 4: Field test on efficacy of aphid control

[0182] The test site is located in the cowpea greenhouse in Shengang Village, Geputan Town, Yunmeng County, Hubei Province. The test site has above-average fertility, flat terrain, medium fertility, and good water conservancy facilities. The plants grow in a relatively consistent manner, and the field fertilizer and water management are basically the same.

[0183] Test target: Aphids.

[0184] Experimental crops: cowpea.

[0185] Experimental design: The experiment set up 6 treatments, each treatment was repeated 4 times, and the area of ​​each plot was 20m 2 , arranged in random blocks; protection rows were retained around the experimental site.

[0186] Test Method: The test involves applying the pesticide once at the initial stage of aphid infestation using a Jacto-HD400 manual sprayer for a single, conventional application. Spraying should be done until both sides of the leaves are wetted without dripping.

[0187] Survey Method: Ten randomly fixed cowpea plants were marked in each plot. Before pesticide application, the total number of aphids on the designated plants was counted as the baseline population. The number of live aphids remaining on the designated plants after pesticide application was also counted 3 and 7 days after application.

[0188] Crop safety survey: After applying the pesticide, observe the growth of cowpeas treated with various pesticides at irregular intervals in the test site to investigate whether any pesticide damage occurs.

[0189] Calculation method of drug efficacy:

[0190]

[0191] Table 5 Results of field aphid control efficacy tests

[0192]

[0193]

[0194] Field efficacy test results (see Table 5) show that both a 32% water-dispersible granule formulation of the compound of Formula I and indazapyroxamet (3:1) and an 18% suspension concentrate of the compound of Formula I and nicofluprole (1:1) demonstrated excellent efficacy in controlling cowpea aphids. Each formulation demonstrated efficacy exceeding 86% on days three and seven after application. Regular observations after application showed that cowpea plants treated with each formulation grew normally, with no phytotoxicity observed.

[0195] Although the present application describes specific embodiments in detail by way of example, the disclosure of the present application may adopt various modifications and alternative forms. However, it should be understood that the disclosure of the present application is not limited to the specific forms disclosed. On the contrary, the disclosure of the present application covers all modifications, equivalents and alternative forms within the scope of the disclosure of the present application, and the scope of the present application is limited by the appended claims and their legal equivalents.

Claims

1. An insecticidal composition, characterized in that The active ingredients of the insecticide composition include active ingredient A and active ingredient B, wherein the active ingredient A is a compound of formula I: (Formula I), wherein the active ingredient B is any one of flupyroxystrobin, indazapyroxamet, and nicofluprole; The active ingredient B is flupyroxystrobin, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 25:1; The active ingredient B is indazapyroxamet, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 18:1; The active ingredient B is nicofluprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:20-25:

1.

2. The insecticidal composition according to claim 1, characterized in that The active ingredient B is flupyroxystrobin, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 16:1; The active ingredient B is indazapyroxamet, and the mass ratio of the active ingredient A to the active ingredient B is 1:10 to 12:1; The active ingredient B is nicofluprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:15-20:

1.

3. The insecticidal composition according to claim 2, characterized in that The active ingredient B is flupyroxystrobin, and the mass ratio of the active ingredient A to the active ingredient B is 1:10 to 12:1; The active ingredient B is indazapyroxamet, and the mass ratio of the active ingredient A to the active ingredient B is 1:5-8:1; The active ingredient B is nicofluprole, and the mass ratio of the active ingredient A to the active ingredient B is 1:10-15:

1.

4. The insecticidal composition according to claim 1, characterized in that The total weight of the insecticide composition is 100 wt %, and the active ingredient A and the active ingredient B account for 1% to 70% of the total weight of the insecticide composition.

5. The insecticidal composition according to claim 1, characterized in that The insecticide composition contains auxiliary ingredients in addition to the active ingredients. The auxiliary ingredients are one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, preservatives, stabilizers, synergists or carriers.

6. The insecticidal composition according to claim 1, characterized in that The insecticide composition is prepared into a formulation form permitted by pesticides, and the formulation form is a solid preparation or a liquid preparation.

7. The insecticidal composition according to claim 6, characterized in that The solid preparation is a water-dispersible granule or a wettable powder, and the liquid preparation is a suspension.

8. Use of the insecticidal composition according to any one of claims 1 to 7 for controlling agricultural, horticultural and forestry pests.

9. The use according to claim 8, characterized in that The pests are dipteran pests or hemiptera pests; the dipteran pests are leafminers, and the hemiptera pests are aphids.

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