A kind of amplicon variant marker for identifying two kinds of horse hoof screws

By using an amplicon variant marker at nucleotide position 214 of the mitochondrial cytochrome oxidase I gene in Rochia nilotica, the problem of morphological indistinguishability between Rochia nilotica and Rochia maxima within the genus Rochia nilotica was solved, achieving highly accurate gene identification.

CN118995959BActive Publication Date: 2026-07-28SOUTH CHINA SEA INST OF OCEANOLOGY CHINESE ACAD OF SCI
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Patent Information

Application Number
CN202411422458.6
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-10-12
Publication Date
2026-07-28
Estimated Expiration
2044-10-12

AI Technical Summary

Technical Problem

Current technology makes it difficult to accurately distinguish between two species within the genus Rochia nilotica and Rochia maxima, as their similar morphology leads to significant identification errors.

Method used

Two species of horseshoe snails were identified by using the amplicon variant marker of the nucleotide sequence at position 214 of the mitochondrial cytochrome oxidase I gene of the horseshoe snail, through direct sequencing, specific probe hybridization, specific primer extension, or PCR.

Benefits of technology

It enables accurate identification of Rochia nilotica and Rochia maxima through gene detection when morphology is difficult to distinguish, and is applicable to the identification of eDNA sequences without physical samples.

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Abstract

The application discloses a kind of amplicon variation markers for identifying two kinds of horse hoof screws.The application finds that there is amplicon variation marker in the position of the 214th position of nucleotide sequence shown in SEQ ID NO.1 of mitochondrial cytochrome oxidase I gene of horse hoof screw, which can distinguish Rochia nilotica and Rochia maxima.If the base of the site is A, the horse hoof screw species is Rochia maxima, and if the base of the site is G, the horse hoof screw species is Rochia nilotica, which is suitable for identifying the two species from eDNA sequence when there is no physical sample, or when the physical form cannot distinguish them.
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