A volatile composition capable of inhibiting Penicillium expansum and its application

Abstract

The present invention discloses a volatile composition capable of inhibiting Penicillium expansum and its application, and belongs to the technical field of Penicillium expansum inhibition. The volatile component composition provided by the present invention is a metabolite of the Kluyveromyces Marxianus YG‑4 yeast strain, which is mainly a small molecule volatile substance with a molecular weight in the range of 50 to 300 Da, has an obvious inhibitory effect on Penicillium expansum, is safe and has no toxic side effects, and is easy to use in batches, and can be widely used in post-harvest disease control of fruits such as apples, pears and kiwis; the Kluyveromyces Marxianus YG‑4 yeast strain and the volatile composition provided can provide a safe, green and environmentally friendly technical option for the reduction of pathogens in the processing of fruits and other foods, and effectively avoid food losses caused by fungal contamination in food base materials and potential safety hazards to human health.

Description

Technical Field

[0001] The invention relates to the technical field of Penicillium expansion inhibition, in particular to a volatile composition capable of inhibiting Penicillium expansion and application thereof. Background Art

[0002] Penicillium expansum is a common spoilage fungus that can cause penicillium infection in fruits, resulting in serious economic losses. Pome fruits (such as apples and pears) and stone fruits (such as cherries, peaches and plums) are the main targets of Penicillium expansum. Legumes (such as beans and peanuts) and cereals (such as corn, barley and sorghum) can also be contaminated by Penicillium expansum. In addition, this species can grow on non-consumable substrates, such as building materials containing cellulose, especially wallpaper and damp wood blocks. Penicillium expansum mainly infects apple fruits through three ways: mechanical damage, natural openings (such as lenticels, stomata, fruit stalks, etc.), and self-secreted enzymes that damage the fruit epidermis to form holes. In the early stage of infection, the pathogen spores are just lurking on the surface of the fruit; when the fruit resistance decreases, the pathogen spores originally lurking on the surface of the fruit enter the fruit through the wound and quickly germinate and express pathogenic factors, initiating the pathogenic mechanism, and finally causing the fruit to rot.

[0003] Traditional methods for inhibiting Penicillium expansum mainly include physical methods and chemical methods, but traditional methods usually have the disadvantages of high cost and drug residues, which in turn affect human health. The present invention proposes a biological control method, in which a volatile composition produced by the metabolism of microbial strains inhibits the growth of Penicillium expansum, and also has a certain inhibitory effect on the growth of some other Aspergillus microorganisms, and can significantly inhibit the growth of Penicillium expansum during apple storage. Summary of the invention

[0004] The purpose of the present invention is to provide a volatile composition capable of inhibiting Penicillium expansum and its application, so as to solve the problems of high cost, large drug residue and potential safety hazards to human health in traditional Penicillium expansum inhibition methods.

[0005] To achieve the above object, the present invention provides an application of a volatile component composition in inhibiting Penicillium expansum, comprising the following substances: phenylethanol, β-phenylethyl butyrate, bis(2-methylpropyl)1,2-phthalate, 2-methyl-3-methylbutyl ester, acetic acid, citronellol, methyl butyl octanoate, ethyl octanoate, 1-butanol 3-methylpropionate, isobutyl undecyl phthalate, isobutyl n-octanoate, octanoic acid, 2-methylbutyl octanoate, 3,3-dimethyl-1,2-epoxybutane, 2-methyl-3-methylbutyl propionate, 2-octanone, 3-methylbutyl heptanoate, 5-decanoic acid, 3-methylbutyl ester, ethyl propionate and 2-methylbutyl decanoate.

[0006] Preferably, the volatile component composition comprises the following substances: phenylethanol, β-phenylethyl butyrate, 3-methylbutyl octanoate, 1-butanol 3-methylpropionate, acetic acid, citronellol, ethyl octanoate and 2-octanone.

[0007] Preferably, the volatile component composition comprises the following substances: phenylethanol, β-phenylethyl butyrate, 3-methylbutyl octanoate, and citronellol.

[0008] Preferably, the volume ratio of phenylethanol: β-phenylethyl butyrate: 3-methylbutyl octanoate: citronellol is 30-50%: 3-10%: 0.1-0.5%: 0.05-0.5%.

[0009] A use of the volatile component composition as described above in inhibiting Penicillium and / or Aspergillus.

[0010] A product comprising the above volatile component composition, wherein the volatile component is separated or artificially synthesized, and the product includes aerosol, aqueous solution, powder, emulsion and oil.

[0011] A use of the product as described above in preventing fruit rot caused by Penicillium and / or Aspergillus.

[0012] The method for producing the above volatile component composition by Kluyveromyces Marxianus YG-4 yeast strain comprises: 8 ~10 9 cfu / dish of Kluyveromyces Marxianus YG-4 yeast strain was spread on YPD medium and cultured at 28°C for 24 to 48 hours. The Kluyveromyces Marxianus YG-4 yeast strain was deposited in China Center for Type Culture Collection, located at 299 Bayi Road, Wuhan University, Wuchang District, Wuhan City, Hubei Province, with the deposit number of CCTCC NO: M20221922.

[0013] The application of Kluyveromyces Marxianus YG-4 yeast strain in inhibiting Penicillium expansum and fruit rot caused by it, the Kluyveromyces Marxianus YG-4 yeast strain is deposited in China Center for Type Culture Collection, located at Wuhan University, No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province, and the collection number is CCTCC NO: M20221922.

[0014] Therefore, the present invention provides a volatile composition capable of inhibiting Penicillium expansum and its application, and its specific technical effects are as follows:

[0015] (1) The volatile component composition provided by the present invention is a metabolite of the Kluyveromyces Marxianus YG-4 yeast strain, which is mainly a small volatile substance with a molecular weight in the range of 50 to 300 Da, has a significant inhibitory effect on Penicillium expansum, is safe and has no toxic side effects, and is easy to use in batches, and can be widely used in the control of post-harvest diseases of fruits such as apples, pears and kiwis;

[0016] (2) The Kluyveromyces Marxianus YG-4 yeast strain and volatile composition provided by the present invention can provide a safe, green and environmentally friendly technical option for reducing pathogens in the processing of fruits and other foods, and effectively avoid food losses and safety hazards to human health caused by fungal contamination in food bases.

[0017] The technical solution of the present invention is further described in detail below through the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for use in the description of the embodiments of the present invention will be briefly introduced below. Obviously, the accompanying drawings in the following description are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative labor.

[0019] Figure 1 is the inhibition rate of different concentrations of Kluyveromyces Marxianus YG-4 strain on Penicillium expansum in Example 2 of the present invention;

[0020] Figure 2 The photographs are of the antagonistic effects of different concentrations of Kluyveromyces Marxianus YG-4 strain on Penicillium expansum on PDA in Example 2 of the present invention;

[0021] Figure 3is the inhibition rate of the volatile composition produced by the Kluyveromyces Marxianus YG-4 strain at different concentrations on Penicillium expansum in Example 3 of the present invention;

[0022] Figure 4 is the inhibitory activity of the simulated volatile composition in Example 5 of the present invention against other fungi of the genus Penicillium and Aspergillus;

[0023] Figure 5 This is the situation in which the Kluyveromyces Marxianus YG-4 strain in Example 6 of the present invention inhibits Penicillium expansum during apple storage;

[0024] Figure 6 This is the situation in which the volatile components of the Kluyveromyces Marxianus YG-4 strain in Example 7 of the present invention inhibit Penicillium expansum during apple storage;

[0025] Figure 7 The inhibition of each simulated composition in Example 8 of the present invention on Penicillium expansum during apple storage;

[0026] Figure 8 This is the situation of the Kluyveromyces Marxianus YG-4 strain in Example 9 of the present invention inhibiting the corruption of apples during storage;

[0027] Fig. 9 This is the situation in which the volatile components of the Kluyveromyces Marxianus YG-4 strain in Example 10 of the present invention inhibit spoilage during apple storage. DETAILED DESCRIPTION

[0028] The technical solution of the present invention is further described below through the accompanying drawings and embodiments.

[0029] In order to make the purpose, technical scheme and advantages of the present application clearer, more thorough and more complete, the technical scheme of the present invention is clearly and completely described below through the accompanying drawings and examples. The following detailed descriptions are all descriptions of the embodiments, and are intended to provide further detailed descriptions of the present invention. Unless otherwise specified, all technical terms used in the present invention have the same meaning as those generally understood by those skilled in the art to which the present application belongs.

[0030] The instruments, equipment, reagents and materials used in the examples were all obtained through commercial channels.

[0031] Embodiment 1

[0032] Kluyveromyces Marxianus YG-4 was obtained by screening, and the specific steps are as follows:

[0033] Weigh 0.5 g of snow lotus fungus pellets, grind them thoroughly under sterile conditions, and dilute them to 10% with sterile saline. 2 , 10 3 and 10 4 cfu / mL, take 100μL of bacterial solution of different concentrations and spread it on YPD solid medium (mass ratio of yeast extract powder: peptone: glucose is 1:2:2, and the amount of agar powder added is 2%), and culture at 28℃. Strain screening process: select a plate with appropriate colony density (30-300cfu / plate), pick a single colony according to the characteristics of yeast colonies (large, thick, round, smooth, and easy to pick up) and spread it on YPD solid medium (mass ratio of yeast extract powder: peptone: glucose is 1:2:2, and the amount of agar added is 2%), continue to purify for three generations, and culture at 28℃ until a single morphology is observed under a microscope. The single morphological strain obtained by screening was sent to the company for sequencing, and the sequencing data was subjected to phylogenetic tree analysis and identification, and it was determined to be a yeast strain, named Kluyveromyces Marxianus YG-4 yeast strain, and preserved in the China Center for Type Culture Collection, located at 299 Bayi Road, Wuchang District, Wuhan City, Hubei Province, Wuhan University, with the collection number CCTCC NO: M20221922.

[0034] Embodiment 2

[0035] The antagonistic ability of the obtained Kluyveromyces Marxianus YG-4 strain to Penicillium expansum was investigated, and the specific steps were as follows:

[0036] The obtained Kluyveromyces Marxianus YG-4 strain was cultured on YPD solid medium (the mass ratio of yeast extract powder: peptone: glucose was 1:2:2, and the amount of agar added was 2%) at 28°C for 24 h. A single colony was picked to prepare a bacterial suspension. 10 μL of different concentrations (10 9 , 10 8 , 10 7 , 10 6 cfu / mL) of Kluyveromyces Marxianus YG-4 bacterial suspension and Penicillium expansum LPH9 spore suspension (10 cfu / mL) were obtained by washing with sterile saline after growing for 7 days. 5spores / mL spore suspension) were added successively into the 5 mm well in the center of potato dextrose agar (PDA, potato: glucose: agar = 10:1:1) plates and cultured at 28°C for 7 days. Three groups of parallel experiments were conducted. The colony diameter was measured by the cross method every day. The plate without Kluyveromyces Marxianus YG-4 bacterial suspension was used as a blank control.

[0037] The results are as follows Figure 2 As shown in the figure, the inhibition rate was calculated using the formula: inhibition rate = (blank group colony diameter - treatment group colony diameter) / blank group colony diameter. The results are shown in the figure. Figure 1 shown.

[0038] Depend on Figure 1 and Figure 2 It can be seen that Kluyveromyces Marxianus YG-4 strain has a significant growth inhibitory effect on Penicillium expansum LPH9, and the higher the concentration of Kluyveromyces Marxianus YG-4, the better the inhibitory effect, and the inhibition rates can reach 50.62%, 62.96%, 100% and 100% respectively. This shows that Kluyveromyces Marxianus YG-4 has a good antagonistic effect on Penicillium expansum LPH9.

[0039] Embodiment 3

[0040] Antibacterial ability of Kluyveromyces Marxianus YG-4 volatile composition against Penicillium expansum

[0041] Different concentrations of Kluyveromyces Marxianus YG-4 strains were spread on YPD medium (yeast extract peptone glucose agar medium, YPD, yeast extract powder: peptone: glucose: agar = 1:2:2:2), and the final number of Kluyveromyces Marxianus YG-4 strains was 10 9 , 10 8 , 10 7 , 10 6 cfu / dish, and after culturing at 28°C for 24 h, 10 μL of Penicillium expansum LPH9 spore suspension (1×10 5spores / mL) were inoculated in the center of the PDA medium, and after natural air drying, they were placed upside down on the YPD medium (both the PDA and YPD medium were without lids) where the Kluyveromyces Marxianus YG-4 strain had been cultured for 24 h. The interface was tightly wrapped with a sealing film and cultured at 28°C for 7 days. Three groups of parallel experiments were conducted, and the colony diameter was measured by the cross method every day. The plate without Kluyveromyces Marxianus YG-4 inoculation was used as a blank control.

[0042] The results are as follows Figure 3 As shown, 10 9 , 10 8 , 10 7 , 10 6 The inhibition rates of Kluyveromyces Marxianus YG-4 on Penicillium expansum LPH9 were 54.93%, 56.34%, 42.25% and 11.27% cfu / dish, respectively, indicating that Kluyveromyces Marxianus YG-4 can produce volatile substances and has an inhibitory effect on the growth of Penicillium expansum LPH9.

[0043] Embodiment 4

[0044] The identification of the volatile composition of Kluyveromyces Marxianus YG-4 strain, the specific steps are as follows:

[0045] Different concentrations of K. Marxianus YG-4 strain were spread on YPD medium, and 10 9 , 10 8 , 10 7 , 10 6 cfu / dish, and YPD plates without K.Marxianus YG-4 strain inoculation were used as blank controls. After 24 hours, the components of volatile gases were determined by gas chromatography-mass spectrometry (QP2010 Ultra, Shimadzu, Japan). The headspace-solid phase microextraction method was used for sampling. The instrument conditions were set as follows: the injection port temperature was 250℃, the carrier gas was helium, the chromatographic column was Agilent DB-1MS, the column flow rate was 1.2mL / min, the injection volume was 1μL, and the injection was not split; the program temperature conditions were: the starting temperature was 40℃, and after holding for 3min, it was heated to 220℃ at a rate of 8℃ / min and held for 3min; the ion source temperature was 230℃, and the quadrupole temperature was 150℃. The ionization method was an electron bombardment source, the electron energy was 70eV, and the full scan mass range was 50-550amu. The computer automatic spectral library NIST14 was used for comparison, and the substances to be tested were qualitatively analyzed.

[0046] The results showed that after subtracting the volatile components of YPD medium, K. Marxianus YG-4 strain could produce a variety of volatile gases. However, the types and contents of gases produced by strains with different concentrations were different. 9 , 10 8 , 10 7 , 10 6 7, 11, 6 and 10 volatile substances were absolutely identified by cfu / dish, respectively, as shown in Tables 1, 2, 3 and 4.

[0047] Table 1 Inoculation volume is 10 9 The volatile components produced by the strain

[0048]

[0049] Table 2 Volatile composition components produced by strains when the inoculation amount is 108

[0050]

[0051]

[0052] Table 3 Volatile composition components produced by strains when the inoculation amount is 107

[0053]

[0054] Table 4 Volatile composition components produced by bacteria when the inoculation amount is 106

[0055]

[0056] Embodiment 5

[0057] Inhibitory activity of simulated volatile compounds against other bacteria of the genus Penicillin and Aspergillus

[0058] Simulation 10 8 The volatile gases of Kluyveromyces Marxianus YG-4 strain with 10 cfu / dish (as shown in Table 5) were dripped onto a 1 cm × 1 cm filter paper, and 10 μL of spore suspensions (1 × 10 5spores / mL) were inoculated in the center of the PDA medium, and after natural air drying, they were inverted on the YPD medium, and the interface was tightly wrapped with a sealing film. They were cultured at 28°C for 7 days. Three groups of parallel experiments were performed, and the colony diameter was measured every day by the cross method. The plate without Kluyveromyces Marxianus YG-4 inoculation was used as a blank control. The inhibition rate was calculated using the formula: inhibition rate = (blank group colony diameter - treatment group colony diameter) / blank group colony diameter.

[0059] Table 5 Simulation 10 8 Volatile composition of Kluyveromyces Marxianus YG-4 strain at cfu / dish

[0060] name Proportion(%) Phenylethyl alcohol 38.13 β-Phenylethyl Butyrate 3.49 3-Methylbutyl octanoate 0.31 Citronellol 0.15

[0061] The results are as follows Figure 4 As shown, 10 8 The inhibition rates of Kluyveromyces Marxianus YG-4 with cfu / dish on Penicillium chrysogenum, Penicillium paneum, Penicillium griseofulvum, Penicillium sp, Aspergillus flavus and Aspergillus ochraceus were 19.44%, 41.03%, 2.54%, 13.33%, 8.33% and 8.90%, respectively, indicating that the volatile composition produced by Kluyveromyces Marxianus YG-4 has a certain inhibitory effect on the growth of different Penicillium and Aspergillus.

[0062] Embodiment 6

[0063] Application of Kluyveromyces Marxianus YG-4 strain in inhibiting Penicillium expansum during apple storage

[0064] Select several healthy apples from the market, rinse them with sterile water and wipe them with medical alcohol (75% ethanol) for disinfection. After drying at room temperature, use a sterile punch to lightly pierce the apples with a circular wound with a diameter of 5 mm and a depth of about 1 cm. Then inoculate 10 μL of Penicillium expansum LPH9 spore suspension (1×10 5 spores / mL) in the circular wound, and then the wound was placed in a plastic box with the wound facing upward to dry. 100 μL of different concentrations of Kluyveromyces Marxianus YG-4 strain (10 9 , 108 , 10 7 / mL), 6 apples per group, incubated at 28℃ for 15 days. The control group was replaced with sterile saline instead of Kluyveromyces Marxianus YG-4 strain, and the incidence and lesion diameters of the experimental and control groups were photographed and recorded. The colony diameter was measured by the cross method, and the inhibition rate was calculated using the formula: inhibition rate = (blank group colony diameter - treatment group colony diameter) / blank group colony diameter.

[0065] The results are as follows Figure 5 As shown, 10 9 , 10 8 , 10 7 The inhibition rates of Kluyveromyces Marxianus YG-4 with cfu / mL against Penicillium expansum LPH9 were 100%, 100%, and 100%, indicating that the bacterial growth of this strain has an antagonistic effect on the rot caused by Penicillium expansum during apple storage.

[0066] Embodiment 7

[0067] Application of volatile components of Kluyveromyces Marxianus YG-4 strain in inhibiting Penicillium expansum during apple storage

[0068] Select several healthy apples from the market, rinse them with sterile water and wipe them with medical alcohol (75% ethanol) for disinfection. After drying at room temperature, use a sterile punch to lightly pierce the apples with a circular wound with a diameter of 5 mm and a depth of about 1 cm. Inoculate 10 μL of Penicillium expansum LPH9 spore suspension (1×10 5 spores / mL) on the circular wound, and then the wound was placed in a plastic box with the wound facing upward to dry. Kluyveromyces Marxianus YG-4 strains (10 9 , 10 8 , 10 7 The plates (1000 × 1000 × 1000) were removed from the cover and placed in a plastic box, where the strain and apples were not in contact. Each group had 6 apples and 6 culture plates, and were cultured at 28°C for 15 days. The control group was a YPD solid plate without Kluyveromyces Marxianus YG-4 strain inoculation. Photos were taken and the lesion diameters of the experimental and control groups were recorded.

[0069] The colony diameter was measured by the cross method, and the inhibition rate was calculated by the formula: inhibition rate = (blank group colony diameter - treatment group colony diameter) / blank group colony diameter. Figure 6 As shown, 10 9 , 108 , 10 7 The inhibition rates of volatile gas components of Kluyveromyces Marxianus YG-4 at cfu / dish on Penicillium expansum LPH9 were 100%, 94.44% and 77.77%, respectively, indicating that the volatile aroma substances of Kluyveromyces Marxianus YG-4 strain had an inhibitory effect on the decay caused by Penicillium expansum during apple storage.

[0070] Embodiment 8

[0071] Application of simulated volatile composition in inhibiting Penicillium expansum during apple storage

[0072] Select several healthy apples from the market, rinse them with sterile water and wipe them with medical alcohol (75% ethanol) for disinfection. After drying at room temperature, use a sterile punch to lightly pierce the apples with a circular wound with a diameter of 5 mm and a depth of about 1 cm. Inoculate 10 μL of Penicillium expansum LPH9 spore suspension (1×10 5 Spores / mL) were placed on the circular wound, and then the wound was placed in a plastic box with the wound facing up to dry. The volatile gases of Kluyveromyces Marxianus YG-4 strain of different concentrations (the components are shown in Tables 6, 7 and 8, respectively) were simulated and dripped on 1 cm × 1 cm filter paper sheets. Six apples and six filter paper sheets were set up in each group and cultured at 28°C for 15 days. Anhydrous ethanol was used instead of citronellol in the control group, and photos were taken and the lesion diameters of the experimental and control groups were recorded.

[0073] The results are as follows Figure 7 As shown, simulation 10 9 , 10 8 , 10 7 The inhibition rates of volatile gas components of Kluyveromyces Marxianus YG-4 at cfu / dish on Penicillium expansum LPH9 were 100%, 82.33% and 72.35%, respectively, indicating that the volatile components of Kluyveromyces Marxianus YG-4 strain had an inhibitory effect on the decay caused by Penicillium expansum during apple storage.

[0074] Table 6 Simulation 10 9 Volatile composition of Kluyveromyces Marxianus YG-4 strain at cfu / dish

[0075] name Proportion(%) Phenylethyl alcohol 48.36 β-Phenylethyl Butyrate 4.63 3-Methylbutyl octanoate 0.15 Citronellol 0.15

[0076] Table 7 Simulation 108 Volatile composition of Kluyveromyces Marxianus YG-4 strain at cfu / dish

[0077] name Proportion(%) Phenylethyl alcohol 38.13 β-Phenylethyl Butyrate 3.49 3-Methylbutyl octanoate 0.31 Citronellol 0.17

[0078] Table 8 Simulation 10 7 Volatile composition of Kluyveromyces Marxianus YG-4 strain at cfu / dish

[0079]

[0080]

[0081] Embodiment 9

[0082] Application of Kluyveromyces Marxianus YG-4 strain in inhibiting apple spoilage during storage

[0083] Select several healthy apples from the market, rinse them with sterile water and wipe them with medical alcohol (75% ethanol) for disinfection. After drying at room temperature, use a sterile punch to lightly pierce the apples with a circular wound with a diameter of 5 mm and a depth of about 1 cm. Inoculate the circular wound with 100 μL of Kluyveromyces Marxianus YG-4 strains (10 9 , 10 8 , 10 7 / mL), 6 apples per group, incubated at 28℃ for 15 days. The control group was replaced with sterile saline instead of Kluyveromyces Marxianus YG-4 strain, and photos were taken and the morbidity and lesion diameter of the experimental group and the control group were recorded.

[0084] The results are as follows Figure 8 As shown, 10 9 , 10 8 , 10 7 The inhibition rates of K. Marxianus YG-4 at cfu / mL on apple spoilage were 100%, 100%, and 85.34%, respectively, indicating that the growth of this strain has an antagonistic effect on apple spoilage during storage.

[0085] Embodiment 10

[0086] Application of volatile components of Kluyveromyces Marxianus YG-4 strain in inhibiting spoilage during apple storage

[0087] Select several healthy apples from the market, rinse them with sterile water and wipe them with medical alcohol (75% ethanol) for disinfection. After drying at room temperature, use a sterile punch to lightly pierce the apples with a circular wound of 5 mm in diameter and about 1 cm in depth. Then stand them in a plastic box with the wound facing upward to dry. 9 , 10 8 , 10 7 The plates (1000 × 1000 × 1000) were removed from the cover and placed in a plastic box, where the strain and apples were not in contact. Each group had 6 apples and 6 culture plates, and were cultured at 28°C for 15 days. The control group was a YPD solid plate without Kluyveromyces Marxianus YG-4 strain inoculation. Photos were taken and the lesion diameters of the experimental and control groups were recorded.

[0088] The results are as follows Fig. 9 As shown, 10 9 cfu / dish, 10 8 cfu / dish, 10 7 The inhibition rates of volatile components of cfu / dish on apple spoilage were 92.56%, 86.57% and 70.25% respectively, indicating that the volatile components of K. Marxianus YG-4 strain had an antagonistic effect on apple spoilage during storage.

[0089] Therefore, the volatile component composition provided by the present invention is a metabolite of the Kluyveromyces Marxianus YG-4 yeast strain, which is mainly a small molecular volatile substance with a molecular weight in the range of 50 to 300 Da, has an obvious inhibitory effect on Penicillium expansum, is safe and has no toxic side effects, is easy to use in batches, and can be widely used in post-harvest disease control of fruits such as apples, pears and kiwis; the provided Kluyveromyces Marxianus YG-4 yeast strain and volatile composition can provide a safe, green and environmentally friendly technical option for reducing pathogens in the processing of fruits and other foods, and effectively avoid food losses caused by fungal contamination in food base materials and safety hazards to human health.

[0090] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that they can still modify or replace the technical solution of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of the present invention.

Claims

1. Use of a volatile component composition in inhibiting Penicillium expansum, characterized in that: The volatile component composition comprises the following substances: phenylethanol, β-phenylethyl butyrate, 3-methylbutyl octanoate, and citronellol; the volume ratio of phenylethanol: β-phenylethyl butyrate: 3-methylbutyl octanoate: citronellol is 30-50%: 3-10%: 0.1-0.5%: 0.05-0.5%.

2. Use of the volatile component composition as claimed in claim 1 for inhibiting Penicillium and / or Aspergillus.

3. Use of the volatile component composition as claimed in claim 1 in preventing fruit rot, characterized in that: Fruit rot is caused by Penicillium and / or Aspergillus species.

4. Kluyveromyces Marxianus The application of volatile substances produced by YG-4 yeast strain in inhibiting Penicillium expansum and fruit rot caused by it is characterized in that: Kluyveromyces Marxianus The YG-4 yeast strain is deposited in China Center for Type Culture Collection, located at 299 Bayi Road, Wuchang District, Wuhan University, Hubei Province, with the deposit number CCTCC NO: M20221922.

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