A kind of anti-hair loss composition containing Polygonum multiflorum extract

Abstract

The invention discloses an anti-hair loss composition, belonging to the field of hair care. The extraction raw materials of the composition include kava black pepper root and ophiopogon erythrorhizon root, giant kelp, black sesame, ginseng, polygonum multiflorum and channa chus; wherein the kava black pepper root extract and the ophiopogon erythrorhizon root extract, giant kelp extract, black sesame extract, ginseng extract, polygonum multiflorum extract and channa chusorhizon extract have significant synergistic effects, and the hair care products prepared from the above raw material extracts have the effects of hair care and anti-hair loss.

Description

Technical Field

[0001] The invention belongs to the field of hair care, and particularly relates to an anti-hair loss composition containing a Polygonum multiflorum extract. Background Art

[0002] Traditional anti-hair loss products such as Deminol may have certain effects in the short term, but long-term use often causes damage to the hair and scalp, and even aggravates the problem of hair loss. In addition, the chemical ingredients in these products may also pose a potential threat to human health. Therefore, it is considered to avoid chemical ingredients and use active substances in plant extracts as the main ingredients. Kava root has entered the field of research and development personnel as an emerging plant extract raw material in the field of anti-hair loss. However, after testing, it was found that kava extract may be irritating to the skin due to its high alkaloid content. Therefore, how to reduce the irritation of kava root and combine kava root extract with other plant extracts to enhance the anti-hair loss and hair care functions has become an urgent problem to be solved. Summary of the invention

[0003] The invention provides an anti-hair loss composition, which has the effects of hair strengthening, hair loss prevention and hair care through natural ingredients extracted from plants.

[0004] In order to achieve the above object, the present invention adopts the following technical solutions:

[0005] In a first aspect, the present invention provides an anti-hair loss composition, characterized in that it comprises the following raw materials in parts by weight:

[0006] 4-6 parts of extract of Psoralea corylifolia root;

[0007] Kava root extract 9-11 parts;

[0008] 2-5 parts of giant kelp extract;

[0009] 2-4 parts of black sesame extract;

[0010] 1-3 parts of ginseng extract;

[0011] 3-5 parts of Polygonum multiflorum root extract;

[0012] 3-5 parts of Channa intestine extract.

[0013] In a second aspect, the present invention provides a method for preparing a kava root extract, and the specific preparation steps are as follows:

[0014] S1, taking a raw material of Piper mesona root, ultrasonically cleaning it to remove impurities, crushing it with a micro-airflow pulverizer, and performing reflux alcohol extraction with a hot reflux extraction and concentration unit to obtain an alcohol extraction product A1 and a residue A2; wherein the alcohol extraction solvent is 40-50% ethanol, the extraction temperature is 40±5° C., the extraction pressure is normal pressure, the extraction method is ethanol reflux extraction, and the reflux times are 4-6 times;

[0015] S2, taking A2, enzymolyzing it with lignin degrading enzyme, cellulase, hemicellulase and pectinase in an enzymolysis pot, filtering it with a bag filter to obtain enzymolysis solution A3; wherein the enzymolysis temperature is 25-32°C, the enzymolysis starting pH is 6-7, the enzymolysis time is 6-8h, the enzymolysis pressure is normal pressure, and the total enzyme dosage is 10%-15% of the mass of the kava root raw material; the enzymolysis step is specifically, first using lignin degrading enzyme, cellulase and hemicellulase for enzymolysis for 4-5h, and then using pectinase for enzymolysis for 1-3h; the mass ratio of the lignin degrading enzyme, cellulase, hemicellulase and pectinase is 1:0.7:0.5:0.7;

[0016] S3, fermenting A3 with Phellinus igniarius in a fermentation pot to obtain a fermentation product A4; wherein the fermentation starting temperature is 30-35° C., the fermentation starting pH is 5-6, the fermentation time is 132-144 h, the amount of bacteria added is ≥ 10% of the mass of the kava root raw material, and the shaking speed is 140-160 r / min;

[0017] S4, adjusting the pH of the unfiltered fermentation product A4 to 6.5-7.5, first enzymolyzing it with glucanase for 4-6 hours, then enzymolyzing it with proteolytic enzyme for 4-6 hours, and then sterilizing, filtering, and freeze-drying to obtain extract A5; wherein the amount of glucanase added is 9% of the mass of the raw material of Piper methysticum root, and the amount of proteolytic enzyme added is 7% of the mass of the raw material of Piper methysticum root;

[0018] S5, merge A1 and A5 to obtain A6.

[0019] In a third aspect, the present invention provides a hair care product ratio and preparation method, characterized in that it contains 0.1wt%-2wt% of the anti-hair loss composition, and the preparation method is as follows:

[0020] T1: Use an emulsifying pot, add surfactant, oil, and cationic regulator into water, heat to 60-75℃, and homogenize;

[0021] T2: When the temperature of the homogenized product in T1 is ≤45°C, add the composition and homogenize;

[0022] T3: Use an autoclave to sterilize the homogenized product of T2, and then use a packaging machine to seal and package the homogenized T2.

[0023] Preferably, the hair care product further comprises a surfactant which is sodium dodecylbenzene sulfonate and / or stearic acid; a fat which is squalane and / or marula oil; and a cationic conditioning agent which is behenyl cetearyl trimethyl ammonium chloride or chitosan quaternary ammonium salt.

[0024] In the present invention:

[0025] Kava root extract: Kava root is subjected to ethanol extraction to obtain an extract product; the extraction residue is then enzymatically hydrolyzed, and then the extraction residue is fermented with Phellinus igniarius, and then the fermentation liquid is enzymatically hydrolyzed with glucanase and proteolytic enzyme. After the extraction step, the Kava root greatly retains the original volatile oil, kava lactone, phenolic and ketone volatile aroma components, and multiple vitamins and minerals, and after Phellinus igniarius fermentation, the secondary metabolites of Phellinus igniarius such as flavonoids, triterpenes, and sterol compounds are added to its original components, and As the Coriolus igniarius ferments, Coriolus igniarius mycelium pellets are obtained, and the Coriolus igniarius mycelium pellets are enzymatically hydrolyzed by glucanase and proteolytic enzymes. The polysaccharides and proteins rich in the mycelium pellets are enzymatically hydrolyzed into small molecules. The kava root can be effectively enriched with ketones, phenols, polysaccharides, sterols and amino acids after the above extraction. The phenols, sterols and ketones contained in the kava root can effectively reduce scalp inflammation, moisturize the scalp and keep it fresh, providing a good living environment for hair follicles. Amino acid compounds can provide hair follicles with more nutrients, help keep them healthy and prevent them from falling off.

[0026] Cynanchum auriculatum Royle ex Wight root extract: Cynanchum auriculatum Royle ex Wight is a herbaceous twining vine of the genus Cynanchum of the Apocynaceae family. Its leaves are rich in nutrients such as amino acids, vitamins, trace elements, and active ingredients such as phospholipids, polysaccharides, and C21 steroids. It has significant anti-inflammatory, anti-tumor, immune-enhancing, lipid-lowering, gastric mucosal protection and other effective effects.

[0027] Giant kelp extract: Giant kelp (Macrocystis Pyrifera) is a plant of the Laminariaceae family, which is rich in amino acids such as aspartic acid, glutamic acid, leucine, etc.; it has significant antioxidant, immunomodulatory, anti-tumor and anticoagulant effects.

[0028] Black sesame extract: Black sesame (Sesamum indicum L) is an extract of the Sesamaceae family, which is rich in fatty oil, lignans, sesame glycosides, folic acid, niacin, protein, cytochrome C, psyllium, sesame sugar, etc.; it has significant antioxidant and anti-cancer effects.

[0029] Ginseng Extract: Panax ginseng CA Mey is the root of Panax ginseng, a plant of the Araliaceae family. It is rich in various ginsenosides, which have the effects of enhancing physical strength, improving immunity, increasing cell activity, and increasing cell migration rate.

[0030] Polygonum multiflorum extract: Polygonum multiflorum (Pleuropterus multiflorus (Thunb.) Nakai) is the dried root of the Polygonum multiflorum plant of the Liao family. It is rich in lecithin, rhein, rhein and other ingredients. It has significant anti-aging, immunity-enhancing, lipid-lowering, antibacterial, and cell activity-enhancing effects.

[0031] Channa intestine extract: Channa intestine (Eclipta prostrata (L.) L.) is a plant of the genus Channa in the Asteraceae family. It is rich in terpenoid compounds, such as 6 / 6 / 6 / 6-tetracyclic triterpenes, pentacyclic triterpenes, pentacyclic triterpenoid saponins, diterpenes and sesquiterpenes. In addition, it also contains active ingredients such as aromatic heterocyclics and steroidal alkaloids. It has the effects of significantly darkening hair, improving immunity and increasing cell activity.

[0032] Compared with the prior art, the invention has the following beneficial effects: the invention innovatively performs alcohol extraction, enzymolysis, fermentation and enzymolysis steps on the kava root; the kava root extract extracted through the above steps is compounded with giant kelp extract and ophiopogon sphaerocephala root extract and supplemented with black sesame extract, ginseng extract, polygonum multiflorum extract and channa dalbergia extract to effectively enhance the hair solidification and anti-hair loss effects; the kava root extract is compounded with giant kelp extract and ophiopogon sphaerocephala root extract and used together with black sesame extract, ginseng extract, polygonum multiflorum extract and channa dalbergia extract, which has a significant synergistic effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 : Example ②-Hair effects of volunteers in week 0.

[0034] Figure 2 : Example ②-Hair effects of volunteers in the 4th week.

[0035] Figure 3 : Example ②-Hair effects of volunteers in the 8th week.

[0036] Figure 4 : Example ②-Hair effects of volunteers in the 12th week. DETAILED DESCRIPTION

[0037] In order to better understand the present invention, the present invention is further described below in conjunction with specific examples, wherein the terms used in the examples are for describing specific embodiments and do not constitute limitations on the scope of protection of the present invention.

[0038] The experimental methods in the following examples without specifying specific conditions are usually carried out under conventional conditions or under conditions recommended by the manufacturer. Unless otherwise specified, percentages and parts are calculated by weight.

[0039] Some raw materials and their sources are as follows:

[0040] Kava root: purchased from Bozhou Huozhengtang Pharmaceutical Co., Ltd.

[0041] Kava root extract: purchased from Xi'an Guohao Biotechnology Co., Ltd.;

[0042] Extract of Psoralea corylifolia root: purchased from Jiangsu Jufeng Chemical Technology Co., Ltd.;

[0043] Giant kelp extract: purchased from Shandong Guante Bioengineering Co., Ltd.;

[0044] Black sesame extract: purchased from Xi'an Guohao Biotechnology Co., Ltd.;

[0045] Ginseng extract: purchased from Xi'an Guohao Biotechnology Co., Ltd.;

[0046] Polygonum multiflorum extract: purchased from Xi'an Guohao Biotechnology Co., Ltd.;

[0047] Channa intestine extract: purchased from Xi'an Guohao Biotechnology Co., Ltd.;

[0048] Sodium dodecylbenzenesulfonate: purchased from Jiangsu Jufeng Chemical Technology Co., Ltd.;

[0049] Marula oil: purchased from Jiangsu Jufeng Chemical Technology Co., Ltd.;

[0050] Chitosan quaternary ammonium salt: purchased from Jiangsu Jufeng Chemical Technology Co., Ltd.;

[0051] Cellulase: purchased from Xi'an Guohao Biotechnology Co., Ltd.;

[0052] Lignin degrading enzyme: purchased from Xi'an Guohao Biotechnology Co., Ltd.;

[0053] Hemicellulase: purchased from Xi'an Guohao Biotechnology Co., Ltd.;

[0054] Pectinase: purchased from Xi'an Guohao Biotechnology Co., Ltd.;

[0055] Sanghuangporus spp: purchased from Ningbo Testo Biotechnology Co., Ltd., catalog number TS327473;

[0056] Inonotus obliquus: purchased from Ningbo Testo Biotechnology Co., Ltd., catalog number TS968202;

[0057] Yeast (Saccharomyces sp.): purchased from Ningbo Testo Biotechnology Co., Ltd., catalog number TS286127;

[0058] Glucanase: β-glucanase, purchased from Shanghai Zhongfeng Biotechnology Co., Ltd.;

[0059] Protease: purchased from Xi'an Guohao Biotechnology Co., Ltd.

[0060] The specific preparation steps of Kava root extract are as follows:

[0061] S1, taking the root of Piper methysticum, cleaning it with an ultrasonic cleaner to remove impurities, and then crushing it with a micro-airflow pulverizer, and then using a hot reflux extraction and concentration unit to perform reflux alcohol extraction to obtain an alcohol extraction product A1 and a residue A2, wherein the alcohol extraction solvent is 40-50% ethanol, the extraction temperature is 40±5° C., the extraction pressure is normal pressure, the extraction method is ethanol reflux extraction, and the reflux number is 4-6 times;

[0062] S2, taking A2, enzymolyzing it with lignin degrading enzyme, cellulase, hemicellulase and pectinase in an enzymolysis pot, filtering it with a bag filter, and obtaining enzymolysis solution A3; wherein the initial enzymolysis temperature is 25-32°C, the enzymolysis pH is 6-7, the enzymolysis time is 6-8h, the enzymolysis pressure is normal pressure, and the enzyme dosage is ≥ 10% of the mass of the kava root raw material; the enzymolysis steps are specifically as follows: firstly, enzymolyzing with lignin degrading enzyme, cellulase and hemicellulase for 4-5h, and then enzymolyzing with pectinase for 1-3h; wherein the mass ratio of lignin degrading enzyme, cellulase, hemicellulase and pectinase is 1:0.7:0.5:0.7;

[0063] S3, fermenting A3 in a fermentation pot with Phellinus igniarius to obtain a fermentation product A4; wherein the fermentation temperature is 30-35° C., the fermentation pH is 5-6, the fermentation time is 132-144 h, the amount of bacteria added is ≥ 10% of the mass of the kava root raw material, and the shaking speed is 140-160 r / min;

[0064] S4, after fermentation, adjusting the pH of the unfiltered fermentation broth to 6.5-7.5, and then enzymolyzing it with glucanase for 4-6 hours, and then enzymolyzing it with proteolytic enzyme for 4-6 hours, and then sterilizing, filtering, and freeze-drying to obtain extract A5; wherein the amount of glucanase added is 9% of the mass of the raw material of Piper methysticum root, and the amount of proteolytic enzyme added is 7% of the mass of the raw material of Piper methysticum root;

[0065] S5, combining A1 and A5 to obtain Kava root extract A6.

[0066] Example 1: Prepared in accordance with S1-S5, wherein in step S1, the ethanol concentration is 40%, the extraction temperature is 40°C, and the reflux times are 5 times; in step S2, the enzymolysis starting temperature is 28°C, the enzymolysis starting pH is 6.0, the enzymolysis time is 7h, and the mass percentage of enzyme added is 15% of the raw material mass; in step S3, the fermentation starting temperature is 32°C, the fermentation starting pH is 5.0, the fermentation time is 140h, the mass percentage of bacteria added is 17% of the raw material mass, and the shaking speed is 148r / min; n; in step S4, the pH of the unfiltered fermentation liquid is adjusted to 6.0, the mass percentage of glucanase added is 9% of the raw material mass, the mass percentage of protease added is 7% of the raw material mass, the glucanase enzymolysis time is 5h, and the protease enzymolysis time is 4h; A6 is the final product.

[0067] Example 2: Prepared in accordance with S1-S5, wherein in step S1, the ethanol concentration is 45%, the extraction temperature is 35°C, and the reflux times are 4 times; in step S2, the enzymolysis starting temperature is 32°C, the enzymolysis starting pH is 6.0, the enzymolysis time is 6h, and the mass percentage of enzyme added is 15% of the raw material mass; in step S3, the fermentation starting temperature is 30°C, the fermentation starting pH is 5.5, the fermentation time is 144h, the mass percentage of bacteria added is 10% of the raw material mass, and the shaking speed is 160r / min; in step S4, the pH of the unfiltered fermentation liquid is adjusted to 7.0, the mass percentage of glucanase added is 9% of the raw material mass, the mass percentage of protease added is 7% of the raw material mass, the glucanase enzymolysis time is 6h, and the protease enzymolysis time is 5h; A6 is the final product.

[0068] Example 3: Prepared in accordance with S1-S5, wherein in step S1, the ethanol concentration is 50%, the extraction temperature is 45°C, and the reflux times are 6 times; in step S2, the enzymolysis starting temperature is 25°C, the enzymolysis starting pH is 7.0, the enzymolysis time is 8h, and the mass percentage of enzyme added is 15% of the raw material mass; in step S3, the fermentation starting temperature is 35°C, the fermentation starting pH is 6.0, the fermentation time is 132h, the mass percentage of bacteria added is 15% of the raw material mass, and the shaking speed is 140r / min; in step S4, the pH of the unfiltered fermentation liquid is adjusted to 7.5, the mass percentage of glucanase added is 9% of the raw material mass, the mass percentage of protease added is 7% of the raw material mass, the glucanase enzymolysis time is 5h, and the protease enzymolysis time is 6h; A6 is the final product.

[0069] Comparative Example 1: Following step S1, A1 is taken as the final product, and its preparation parameters are the same as those of Example 2.

[0070] Comparative Example 2: Following steps S1 and S2, A3 is obtained as the final product, and its preparation parameters are the same as those of Example 2.

[0071] Comparative Example 3: Following steps S1, S2, and S3, A4 is obtained as the final product, and its preparation parameters are the same as those of Example 2.

[0072] Comparative Example 4: Following steps S1, S2, and S3, A5 is obtained as the final product, and its preparation parameters are the same as those of Example 2.

[0073] Comparative Example 5: The lignin degrading enzyme, cellulase, hemicellulase and pectinase in Example 2 were replaced by cellulase, and the other steps were the same as in Example 2.

[0074] Comparative Example 6: The Phellinus linterus in Example 2 was replaced by Inonotus obliquus, and the other steps were the same as those in Example 2.

[0075] Comparative Example 7: The step A4 of S3 in Example 2 was not subjected to enzymatic hydrolysis with glucanase and proteolytic enzyme, and the remaining steps were the same as those in Example 2.

[0076] Comparative Example 8: The Piper mesenteriae root in step S1 was replaced with Piper mesenteriae root, and the other steps were the same as those in Example 2.

[0077] The weight ratio of raw materials of an anti-hair loss composition is specifically shown in Table 1:

[0078] Table 1

[0079] Experimental Group Kava Root Extract serial number Extract of Psoralea corylifolia Root Giant kelp extract Black sesame extract Ginseng Extract Polygonum multiflorum extract Channa Intestine Extract Kava root extract (commercially available) Example 4 4 Example 2 11 3 2 1 3 4 0 Example 5 5 Example 2 10 5 4 2 5 3 0 Example 6 6 Example 2 9 2 3 3 4 5 0 Example 7 5 Example 1 10 5 4 2 5 3 0 Example 8 5 Example 3 10 5 4 2 5 3 0 Comparative Example 7 5 Comparative Example 1 10 5 4 2 5 3 0 Comparative Example 8 5 Comparative Example 2 10 5 4 2 5 3 0 Comparative Example 9 5 Comparative Example 3 10 5 4 2 5 3 0 Comparative Example 10 5 Comparative Example 4 10 5 4 2 5 3 0 Comparative Example 11 5 Comparative Example 5 10 5 4 2 5 3 0 Comparative Example 12 5 Comparative Example 6 10 5 4 2 5 3 0 Comparative Example 13 0 none 10 5 4 2 5 3 5 Comparative Example 14 5 Example 2 0 5 4 2 5 3 0 Comparative Example 15 5 Example 2 10 0 4 2 5 3 0 Comparative Example 16 0 0 0 0 4 2 5 3 0 Comparative Example 17 0 Example 2 10 5 4 2 5 3 0 Comparative Example 18 5 Comparative Example 7 10 5 4 2 5 3 0 Comparative Example 19 5 Comparative Example 8 10 5 4 2 5 3 0

[0080] A hair care product, the specific mass percentage ratio of which is shown in Table 2:

[0081] Table 2

[0082]

[0083] Note: Make up the remainder with water.

[0084] Safety Test

[0085] With reference to the 2015 Technical Specification for Safety of Cosmetics, the hair care products of Examples ①-⑦ were evaluated for cosmetic irritation. The test method was a skin patch test, and 70 randomly distributed people aged 16-65 were tested.

[0086] Test method: Put the test substance into the patch tester, the dosage is 0.020-0.025g, cover the patch tester with the test substance on the back or forearm of the subject with non-irritating cloth-based tape, and gently press it with the palm to make it evenly adhere to the skin surface for 24 hours. After removing the test patch tester, observe the skin reaction after the indentation disappears 30 minutes later. If the result is negative, observe again 24 hours and 48 hours after the patch test.

[0087] Evaluation criteria:

[0088] Grade 0: negative reaction;

[0089] Grade 1: Suspicious reaction, only slight erythema;

[0090] Grade 2: weak positive reaction, erythema, infiltration, edema, and papules may be present;

[0091] Grade 3: Strong positive reaction, erythema, infiltration, edema, papules, and the reaction may extend beyond the test area;

[0092] Grade 4: Very strong positive reaction, obvious erythema, severe infiltration, edema, confluent herpes, and the reaction exceeds the test area.

[0093] Test results: Skin reactions were negative in all subjects.

[0094] The above test results show that the hair care product containing the anti-hair loss composition provided by the present invention is mild and non-irritating to the skin and is safe to use.

[0095] In vitro anti-hair loss test

[0096] This experiment is based on Examples ①-⑦ and Comparative Examples ①- The sample was used to detect the expression of VEGF in hair papilla cells.

[0097] Dermal papilla cells are a group of dermal-derived cells located at the base of hair follicles. They play a key role in regulating hair follicle growth by secreting many bioactive factors such as vascular endothelial growth factor (VEGF).

[0098] This test is to establish a dermal papilla cell model in vitro, intervene in the growth process by adding samples, detect changes in VEGF expression, and evaluate the anti-hair loss efficacy of the samples.

[0099] Main reagents: minoxidil (positive control), mesenchymal stem cell-specific culture medium, 0.25% trypsin solution, phosphate buffered saline (PBS), CCK-8, Human VEGF ELISA Kit.

[0100] Cells used: Human dermal papilla cells (HHDPC).

[0101] Efficacy evaluation concentration design: Blank control group: mesenchymal stem cell-specific culture medium; Positive control: minoxidil: Sample group: Examples ①-⑦ and Comparative Examples ①- .

[0102] Experimental method: Sample group: After culturing HHDPC cells for 12 hours, add 2μL, 0.2% sample, continue culturing for 72 hours, and then use Human VEGF ELISA Kit to detect the expression of VEGF in cells. For specific detection methods, refer to the instructions for use of the kit.

[0103] Positive group: After culturing HHDPC cells for 12 hours, add 2 μL of 20 μg / mL minoxidil, continue culturing for 72 hours, and then use Human VEGF ELISA Kit to detect the expression of VEGF in cells. For specific detection methods, refer to the instructions for use of the kit.

[0104] Blank group: After culturing HHDPC cells for 12 hours, add 2 μL of mesenchymal stem cell-specific culture medium, continue culturing for 72 hours, and then use Human VEGF ELISA Kit to detect the expression of VEGF in cells. For specific detection methods, refer to the instructions for use of the kit.

[0105] Efficacy judgment: When the VEGF expression in the sample group is significantly increased compared with the blank group (P < 0.05), it can be judged that the sample group has the effect of preventing hair loss at this concentration. The specific results are shown in Table 3.

[0106] Table 3

[0107]

[0108] According to the results in Table 3, among the embodiments ①-⑦, the embodiment ② has the best effect. , The results show that the kava root extract prepared by the extraction process defined in the present invention and the combination of the Auricularia auricula root extract, the giant kelp extract, the black sesame extract, the ginseng extract, the Polygonum multiflorum extract and the Channa intestine extract can effectively strengthen hair and prevent hair loss.

[0109] Comparison of the results of Example ② and Comparative Example ⑦ shows that the Kava root extract prepared by the extraction process defined in the present invention has a significant anti-hair loss and hair solidification effect compared to the traditional Kava root extract available on the market.

[0110] Comparison of Example ② and Comparative Example ⑧ The results show that there is a significant synergistic effect between the kava root extract defined in the present invention and the Auricularia auricula root extract, giant kelp extract, black sesame extract, ginseng extract, Polygonum multiflorum extract, and Channa intestine extract.

[0111] Comparison of Example ② and Comparative Example The results showed that excessive addition of the anti-hair loss composition may lead to a decrease in its hair solidifying effect.

[0112] Comparison of Example ② and Comparative Example The results show that the anti-hair loss composition provided by the present invention can effectively prevent hair loss and has significant anti-hair loss and hair strengthening effects.

[0113] Subjective human perception experiment

[0114] The subjects were not allowed to wash their hair within 48h±5h before each test, and were not allowed to comb their hair on the day of the test. After arriving at the test site and acclimatizing to the environment each time, the same trained technician used a comb of the same specifications to comb their hair evenly 60 times (30 times on each side) in the order from left front to left back and right front to right back. The fallen hair was then collected and counted.

[0115] 190 healthy male or female subjects aged 18-60 with severe hair loss and mild hair thinning, with hair length between 5-40 cm, and with more than 10 hair roots lost according to the 60-times combing method were screened and divided into 19 groups, with 10 subjects in each group. The subjects were respectively subjected to the hair care products of Examples ①-⑦ and Comparative Examples ①-⑮, once in the morning and once in the evening, and each use was performed according to the instructions, and the scalp was massaged for 1-3 minutes during the washing process. The subjects were respectively subjected to the 60-times combing method before use and 4 weeks, 8 weeks, and 12 weeks after use, and the number of hair roots lost was tested and recorded. The weekly hair loss reduction rate % = (number of hair roots lost before use - number of hair roots lost after 12 weeks of use) / number of hair roots lost before use * 100%.

[0116] Table 4

[0117]

[0118] According to the results in Table 4, among the embodiments ①-⑦, the embodiment ② has the best effect. , The results show that the kava root extract prepared by the extraction process defined in the present invention and the combination of the Auricularia auricula root extract, the giant kelp extract, the black sesame extract, the ginseng extract, the Polygonum multiflorum extract and the Channa intestine extract can effectively strengthen hair and prevent hair loss.

[0119] Comparison of the results of Example ② and Comparative Example ⑦ shows that the Kava root extract prepared by the extraction process defined in the present invention has a significant anti-hair loss and hair solidification effect compared to the traditional Kava root extract available on the market.

[0120] Comparison of Example ② and Comparative Example ⑧ The results show that there is a significant synergistic effect between the kava root extract defined in the present invention and the Auricularia auricula root extract, giant kelp extract, black sesame extract, ginseng extract, Polygonum multiflorum extract, and Channa intestine extract.

[0121] Comparison of Example ② and Comparative Example The results showed that excessive addition of the anti-hair loss composition may lead to a decrease in its hair solidifying effect.

[0122] Comparison of Example ② and Comparative Example The results show that the anti-hair loss composition provided by the present invention can effectively prevent hair loss and has significant anti-hair loss and hair strengthening effects.

[0123] The embodiments described above are part of the embodiments of the present application, rather than all of the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the present application for protection, but merely represents the selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the present application.

Claims

1. An anti-hair loss composition, characterized in that The composition comprises the following raw materials in parts by weight: 4-6 parts of extract of Psoralea corylifolia root; Kava root extract 9-11 parts; 2-5 parts of giant kelp extract; 2-4 parts of black sesame extract; 1-3 parts of ginseng extract; 3-5 parts of Polygonum multiflorum root extract; 3-5 parts of Channa intestine extract; The preparation method of the Kava root extract is as follows: S1, taking kava root raw material, crushing, and alcohol extraction to obtain alcohol extraction product A1 and residue A2; the alcohol extraction solvent is 40-50% ethanol, the extraction temperature is 40±5°C, the extraction pressure is normal pressure, the extraction method is ethanol reflux extraction, and the reflux times are 4-6 times; S2, taking A2, enzymolyzing it with lignin degrading enzyme, cellulase, hemicellulase, and pectinase, filtering it, and obtaining enzymolysis solution A3; wherein the enzymolysis temperature is 25-32°C, the enzymolysis pH is 6-7, the enzymolysis time is 6-8h, the enzymolysis pressure is normal pressure, and the enzyme dosage is ≥ 10% of the mass of the raw material of Piper methysticum root; S3, fermenting A3 with Phellinus igniarius to obtain a fermentation product A4; wherein the fermentation temperature is 30-35° C., the fermentation pH is 5-6, the fermentation time is 132-144 h, the amount of bacteria added is ≥ 10% of the mass of the raw material of Piper methysticum root, and the shaking speed is 140-160 r / min; S4, after fermentation, adjusting the pH of the unfiltered fermentation broth to 4.5-7.5, and then enzymolyzing it with glucanase for 4-6 hours, and then enzymolyzing it with proteolytic enzyme for 4-6 hours, and then sterilizing it to obtain extract A5; S5, merge A1 and A5 to obtain A6.

2. A hair care product, characterized in that: The anti-hair loss composition according to claim 1 comprises 0.1 wt% to 2 wt%.

3. The hair care product according to claim 2, characterized in that: It also includes 1wt% surfactant, 15wt% oil and fat, and 3wt% cationic adjuster.

4. The hair care product according to claim 3, characterized in that: The surfactant is sodium dodecylbenzene sulfonate and / or stearic acid; the oil is squalane and / or marula oil; and the cationic conditioning agent is behenyl cetearyl trimethyl ammonium chloride and / or chitosan quaternary ammonium salt.

5. The hair care product according to claim 4, characterized in that: The steps of preparing the hair care product are as follows: T1: Add surfactant, oil and cationic regulator into water, heat to 60-75℃ and homogenize; T2: When the temperature of the homogenized product in T1 is ≤45°C, add the anti-hair loss composition and homogenize; T3: Sterilize the homogenized product in T2 and seal it.

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