A single-chain antibody against LMBV and its application in preparation of quantum dot fluorescent immunochromatographic test strip

The fluorescent immunochromatography technology that combines anti-LMBV single-chain antibodies with fluorescent quantum dot markers solves the problems of low sensitivity, weak specificity and complex operation of LMBV detection in existing technologies, and achieves rapid, efficient and economical virus detection, which is suitable for on-site detection of largemouth bass iridovirus.

CN119039426BActive Publication Date: 2025-10-17NORTHWEST A & F UNIV
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Patent Information

Application Number
CN202411457247.6
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2024-10-18
Publication Date
2025-10-17
Estimated Expiration
2044-10-18

AI Technical Summary

Technical Problem

Existing technologies for largemouth bass iridovirus (LMBV) detection have problems such as low sensitivity, weak specificity, complex operation, high cost, and unsuitability for rapid detection. Especially in aquaculture environments, it is difficult to meet the needs of fast, efficient, and accurate detection.

Method used

The fluorescent immunochromatography technology uses anti-LMBV single-chain antibody combined with fluorescent quantum dot markers to recognize and bind to the virus through highly specific antibodies, and uses fluorescent quantum dot-labeled antibodies to improve the sensitivity and accuracy of detection and simplify the operation steps.

Benefits of technology

It achieves rapid, efficient, economical and highly specific detection of LMBV, simplifies the operation process, and is suitable for on-site application, especially in providing rapid decision-making support in customs quarantine and sudden epidemic monitoring.

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Abstract

The application discloses an anti-LMBV single-chain antibody and application thereof in preparation of a quantum dot fluorescent immunochromatography test strip, and belongs to the technical field of biological detection. The single-chain antibody comprises Ab-K1 and Ab-K2, the amino acid sequence of the Ab-K1 is shown as SEQ ID NO. 11, and the amino acid sequence of the Ab-K2 is shown as SEQ ID NO. 12. The single-chain antibody used in the application can accurately recognize and combine with iridovirus in a sample to be detected, so that the accuracy of detection is ensured. Meanwhile, the antibody labeled with fluorescent quantum dots has good fluorescent characteristics, so that the fluorescent signal is easy to observe and detect, and the specific surface area is relatively large, so that the antibody can be combined with more virus molecules, so that the sensitivity of detection is improved, the accuracy and sensitivity of detection are improved, the operation steps are simplified, and powerful technical support is provided for detection and prevention and control of iridovirus.
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