Transparency detector and transparency detection system

Abstract

The present application relates to a transparency detector and a transparency detection system. The transparency detector comprises: a bottom transparent plate, a sample imaging platform, a bottom surface light source, a camera and a bracket; the bottom surface light source is placed between the sample imaging platform and the bottom transparent plate, and the camera is connected to the bracket; the bottom transparent plate comprises: a light-transmitting layer, a light-shielding layer arranged on the light-transmitting layer; the light-shielding layer has through holes arranged in rows and columns, and mutually perpendicular transverse scales and longitudinal scales, both of which are light-transmitting, the aperture of the through holes is less than 1mm, and the spacing between the through holes is not more than 10mm; the sample imaging platform is used to carry the bottom surface light source and the bottom transparent plate; the light emitted by the bottom surface light source passes through the light-transmitting layer and the through holes and irradiates the tissue placed on the bottom transparent plate; the camera is used to take pictures of the tissue placed on the bottom transparent plate, and the terminal device determines the transparency of the tissue based on the image. The present application can improve the accuracy of transparency detection.

Description

Technical Field

[0001] The present application relates to the technical field of detection equipment, and more specifically, to a transparency detector and a transparency detection system. Background Art

[0002] Transparency, as a physical property of tissue, is directly related to the penetration depth of light in the tissue and the quality of imaging, and has a decisive influence on the application effect of optical imaging technology. For example, in the diagnosis of ophthalmic diseases, the change in transparency of retinal tissue is a key indicator for evaluating various retinal diseases; in oncology research, the difference in transparency between tumor tissue and its surrounding normal tissue can provide an important basis for early diagnosis and evaluation of treatment effects.

[0003] Currently, the detection of tissue transparency mainly relies on the intuitive judgment of doctors. This method has the disadvantages of strong subjectivity, poor accuracy, and difficulty in quantification.

[0004] Therefore, developing a transparency detector that can accurately, quickly and quantitatively detect tissue transparency and is easy to operate and low-cost has become an urgent problem to be solved. Summary of the invention

[0005] The present application is provided to solve the above problems existing in the prior art. According to the transparency detector and the transparency detection system of the embodiments of the present application, the accuracy of transparency detection can be improved.

[0006] According to a first aspect of the present application, there is provided a transparency detector, comprising: a bottom transparent plate, a sample imaging table, a bottom surface light source, a camera and a bracket;

[0007] The bottom surface light source is placed between the sample imaging platform and the bottom transparent plate, and the camera is connected to the bracket;

[0008] The bottom transparent plate comprises: a light-transmitting layer and a light-shielding layer arranged on the light-transmitting layer;

[0009] The light shielding layer has through holes arranged in rows and columns, and transverse scales and longitudinal scales perpendicular to each other, a line connecting the center points of the through holes in each row is parallel to the transverse scale, a line connecting the center points of the through holes in each column is parallel to the longitudinal scale, the transverse scale and the longitudinal scale are both light-transmissive, the aperture of the through holes is less than 1 mm, and the spacing between the through holes is not more than 10 mm;

[0010] The sample imaging platform is used to carry the bottom surface light source and the bottom transparent plate;

[0011] The light emitted by the bottom surface light source passes through the light-transmitting layer and the through hole and irradiates the tissue placed on the bottom transparent plate;

[0012] The camera is used to take pictures of the tissue placed on the bottom transparent plate, and the taken images are provided to the terminal device so that the terminal device determines the transparency of the tissue based on the images.

[0013] According to a second aspect of the present application, there is provided a transparency detection system, comprising: a terminal device and the transparency detector described in any one of the above embodiments;

[0014] The terminal device is used to determine the transparency of the tissue based on the image taken by the camera.

[0015] By using the transparency detector of each embodiment of the present application, since the through hole of the bottom transparent plate has a smaller size, the camera can obtain a tissue image with a smaller analysis unit, and the terminal device can calculate a more accurate transparency based on the image to achieve quantitative detection of tissue transparency. This process can be automatically performed by the instrument, does not rely on manual subjective judgment, and has higher detection efficiency and credibility. At the same time, based on the translucent horizontal and vertical scales on the bottom transparent plate, the through hole can be accurately located to improve the accuracy of transparency detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In the drawings, which are not necessarily drawn to scale, the same reference numerals may describe similar parts in different views. The same reference numerals with letter suffixes or different letter suffixes may represent different instances of similar parts. The accompanying drawings generally illustrate various embodiments by way of example and not limitation, and together with the specification and claims, are used to illustrate the disclosed embodiments. When appropriate, the same reference numerals are used throughout the drawings to refer to the same or similar parts. Such embodiments are illustrative and are not intended to be exhaustive or exclusive embodiments of the present apparatus or method.

[0017] Figure 1 It is a structural schematic diagram of a transparency detector provided by an embodiment of the present application;

[0018] Figure 2 is a top view of a bottom transparent plate provided by an embodiment of the present application;

[0019] Figure 3 is a side view of a bottom transparent plate provided by an embodiment of the present application;

[0020] Figure 4 is a structural schematic diagram of a transparency detector provided by another embodiment of the present application;

[0021] Figure 5 is a partially enlarged schematic diagram of a bottom transparent plate provided by an embodiment of the present application;

[0022] Figure 6 It is a partially enlarged schematic diagram of a bottom transparent plate provided in another embodiment of the present application.

[0023] Figure 7 This is a schematic diagram of shooting before tissue placement provided by an embodiment of the present application;

[0024] Figure 8 It is an image taken before placing the tissue provided by one embodiment of the present application;

[0025] Fig. 9 This is a schematic diagram of photographing after tissue placement provided by an embodiment of the present application;

[0026] Fig.10 This is an image taken after tissue placement provided by an embodiment of the present application. DETAILED DESCRIPTION

[0027] In order to enable those skilled in the art to better understand the technical solution of the present application, the present application is described in detail below in conjunction with the accompanying drawings and specific embodiments. The embodiments of the present application are further described in detail below in conjunction with the accompanying drawings and specific embodiments, but are not intended to limit the present application. The terms "first", "second" and "third" used in the present application are only intended to distinguish the corresponding features, do not represent the need for such an order, and do not necessarily represent only the singular form.

[0028] like Figure 1 As shown, an embodiment of the present invention provides a transparency detector, comprising: a bottom transparent plate 10, a sample imaging platform 20, a bottom surface light source 30, a camera 40 and a bracket 50. The bracket 50 is used to support the camera 40 to ensure the stability of shooting and improve the image shooting quality. The bottom surface light source 30 can be embedded in the sample imaging platform 20, or fixed to the sample imaging platform 20 by a fixing member.

[0029] The bottom surface light source 30 is placed between the sample imaging stage 20 and the bottom transparent plate 10 , and the camera 40 is connected to the bracket 50 .

[0030] The sample imaging platform 20 is used to carry the bottom surface light source 30 and the bottom transparent plate 10 .

[0031] The light emitted by the bottom surface light source 30 passes through the light-transmitting layer and the through hole of the bottom transparent plate 10 and irradiates the tissue placed on the bottom transparent plate 10. The tissue may be placed on the bottom transparent plate through a glass slide.

[0032] The camera 40 is used to take pictures of the tissue placed on the bottom transparent plate 10, and the taken images are provided to the terminal device so that the terminal device determines the transparency of the tissue based on the images before and after the placement of the tissue.

[0033] like Figure 2 and Figure 3As shown, the bottom transparent plate 10 includes a light-transmitting layer 15 and a light-shielding layer 14 disposed on the light-transmitting layer.

[0034] The light shielding layer 14 has through holes arranged in rows and columns, that is, Figure 2 The light-transmitting micro-holes 13 shown in the figure, the light-shielding layer also has a transverse scale 11 and a longitudinal scale 12 which are perpendicular to each other.

[0035] A line connecting the center points of the through holes 13 in each row is parallel to the transverse scale 11 , and a line connecting the center points of the through holes 13 in each column is parallel to the longitudinal scale 12 .

[0036] Both the transverse scale 11 and the longitudinal scale 12 are light-transmissive.

[0037] The diameter of the through holes 13 is less than 1 mm, and the spacing between the through holes 13 is no more than 10 mm.

[0038] In the embodiment of the present application, since the through hole of the bottom transparent plate has a smaller size, the camera can obtain a tissue image with a smaller analysis unit, and the terminal device can calculate a more accurate transparency based on the image to achieve quantitative detection of tissue transparency. This process can be automatically performed by the instrument, does not rely on manual subjective judgment, and has higher detection efficiency and credibility. At the same time, based on the translucent horizontal and vertical scales on the bottom transparent plate, the through hole can be accurately located to improve the accuracy of transparency detection.

[0039] In one embodiment of the present application, reference Figure 4 In the bracket shown in the figure, the bracket 50 includes a connecting block 51, a lateral moving mechanism 52 and a longitudinal moving mechanism 53. The lateral moving mechanism 52 can move on the longitudinal moving mechanism 53.

[0040] The camera 40 is fixed on the connecting block 51, and the connecting block 51 can move on the transverse moving mechanism 52, and its moving direction is perpendicular to the moving direction of the transverse moving mechanism 51 on the longitudinal moving mechanism 52, that is, one moves along the X-axis and the other moves along the Y-axis.

[0041] Through the lateral movement mechanism and the longitudinal movement mechanism, the camera can achieve changes in lateral and longitudinal positions, adjust the shooting position, and improve image quality. In actual application scenarios, there may be only a lateral movement mechanism or a longitudinal movement mechanism, and the lateral movement mechanism or the longitudinal movement mechanism may have a lifting rod to enable the camera to move up and down while moving lateral or longitudinally. Of course, the bracket may also only include a lifting rod, so that the camera only has the ability to move up and down.

[0042] In one embodiment of the present application, the transparency detector may further include: a top surface light source, a reference Figure 4The top surface light source 70 is shown in the figure. The top surface light source 70 is connected to the connecting block 51 of the bracket and is used to fill in the light for the tissue. In order to achieve a better fill-in light effect, the position of the top surface light source is lower than the camera, such as 10 mm lower, that is, the top surface light source is closer to the bottom transparent plate, and the light of the top surface light source is not parallel to the plane where the bottom transparent plate is located. Since the light emitted by the bottom surface light source and the top surface light source is distributed in a planar form in space, it can provide more uniform and soft light, so that the illuminated area is more evenly illuminated.

[0043] In one embodiment of the present application, the transparency detector may further include: a lifting mechanism, referring to Figure 4 The lifting mechanism 60 is shown in FIG. The sample imaging platform 20 is connected to the lifting mechanism 60 , and the sample imaging platform 20 is raised or lowered by the lifting mechanism 60 .

[0044] Through the lifting mechanism, the tissue is brought closer to the camera, so that smaller tissue units and more tissue details can be presented in the image. Similar to the camera, the sample imaging stage can also achieve lateral or longitudinal movement through the lateral telescopic rod and the longitudinal telescopic rod. For example, the sample imaging stage is fixed on the lateral telescopic rod, and the lateral telescopic rod is fixed on the lifting mechanism. The lifting mechanism can drive the sample imaging stage to rise and fall through the lateral telescopic rod, and the lateral telescopic rod can drive the sample imaging stage to achieve lateral movement.

[0045] In one embodiment of the present application, the transparency detector may further include a bottom transparent plate fixing block 80, which may fix the bottom transparent plate on the sample imaging platform 20 by clamping or the like, thereby ensuring the stability of the bottom transparent plate during the shooting process, improving the image quality, and thereby improving the analysis accuracy.

[0046] In one embodiment of the present application, the transparency detector further includes: a light-shielding housing, which is not shown in the accompanying drawings. The bottom transparent plate, the sample imaging table, the bottom surface light source, the camera and the bracket are located in the light-shielding housing. In order to avoid interference from external light sources, the present embodiment provides a light-shielding housing, which can be made of plastic or the like. By providing a light-shielding housing, the transparency detector can work in different environments, broadening its application scenarios.

[0047] In addition, the top surface light source, the lifting mechanism and the bottom transparent plate fixing block can also be placed in the light shielding shell.

[0048] In one embodiment of the present application, the bottom transparent plate can be divided into different detection areas, each detection area has a different detection accuracy. For example, from area 1 to area 3, the diameter of the through holes gradually increases, and the spacing gradually increases. Therefore, the detection accuracy of area 1 is the highest. In the same detection area, the size of the through holes is the same, the lateral spacing between the through holes is the same, and the longitudinal spacing between the through holes is the same. The lateral spacing and the longitudinal spacing can be the same or different. Through different detection areas, different tissues can be detected and compared at the same time, and different areas of the same tissue can also be detected and compared to suit different business scenarios.

[0049] It should be noted that, in actual application scenarios, the vertical scale and the horizontal scale may include only scale lines, or may include both scale lines and digital identifiers.

[0050] In one embodiment of the present application, in order to make the scale display more obvious, the horizontal scale and the vertical scale are hollow structures, wherein the edges of the scale lines and the digital logos can be hollowed out, or the bodies of the scale lines and the digital logos can be hollowed out. Of course, the scale lines and the digital logos can also be formed by transparent materials embedded in the light-shielding layer, and are not limited to one implementation method.

[0051] In one embodiment of the present application, the spacing between the through holes is [20 μm-10 mm]. For example, the spacing between the through holes is 20 μm, or 10 mm, or 1 mm, etc. Through the embodiment of the present application, the through hole spacing can reach the micron level, thereby achieving more accurate detection. If the lateral spacing is different from the longitudinal spacing, the lateral spacing and the longitudinal spacing are both within the range of [20 μm-10 mm].

[0052] In one embodiment of the present application, the aperture of the through hole is [2μm-1mm), such as 2μm, or 50μm, or 0.99mm. If the through hole is circular, its diameter is [2μm-1mm), if the through hole is elliptical, its major axis is [2μm-1mm), if the through hole is square, its side length is [2μm-1mm). The embodiment of the present application reduces the size of the through hole and reduces the minimum detectable area unit, thereby improving the detection accuracy.

[0053] In one embodiment of the present application, the shape of the through hole can be a regular shape such as a circle, a triangle, a square, a rectangle, an ellipse, a five-pointed star, etc., to facilitate subsequent quantitative calculations and improve the efficiency of quantitative calculations.

[0054] In one embodiment of the present application, the light-transmitting layer is any one of quartz glass, optical glass and polymer transparent material. Among them, the polymer transparent material can be polystyrene, acrylonitrile-styrene copolymer, acrylonitrile-butadiene-styrene copolymer, polycarbonate, etc. Taking quartz glass as an example, as a light-transmitting layer, it has high transparency, high stability and high strength.

[0055] In one embodiment of the present application, the light shielding layer is a chrome-plated layer. The light shielding layer formed by chrome plating can effectively reduce the penetration of light and achieve a good light shielding effect. Chromium has good chemical stability, heat resistance and wear resistance, and can maintain flatness and light shielding effect for a long time.

[0056] In one embodiment of the present application, in addition to electroplating, the light shielding layer can also be attached to the light-transmitting layer by coating or spraying. For example, the coating material can be a polyethylene film that has been subjected to surface treatment such as corona treatment, or a polypropylene film, etc. The sprayed light shielding material can be polystyrene particles, nanomaterials, etc.

[0057] The through holes can be prepared by etching, laser drilling, etc. The bottom transparent plate processed in this way has high precision of through holes and hole spacing, which can achieve high-precision transparency detection, and the through holes are not easily blocked by tissue samples and are easy to clean.

[0058] In actual application scenarios, the transparency of the tissue can be judged by analyzing the changes in parameters such as brightness, center of mass displacement, number, and morphology (such as roundness, aspect ratio, and symmetry) of each through hole in the bottom transparent plate, thereby achieving the purpose of accurately quantifying the transparency of the tissue.

[0059] In one embodiment of the present application, reference Figure 5 , the through hole on the bottom plate, that is Figure 5 The light-transmitting holes (black dots) shown in the figure are circular holes with an aperture of 100 μm, a lateral spacing of 0.5 mm and a longitudinal spacing of 1 mm between the through holes.

[0060] In one embodiment of the present application, Figure 6 As shown, the aperture of the through hole is 50 μm, and the spacing between the through holes is 0.5 mm, that is, the lateral spacing and the longitudinal spacing are 0.5 mm.

[0061] An embodiment of the present invention provides a transparency detection system, comprising: a terminal device and a transparency detector according to any one of the above embodiments.

[0062] A terminal device for determining the transparency of tissue based on images captured by a camera.

[0063] like Figure 7 As shown, a camera is used to photograph the bottom plate before placing the tissue, and the resulting image is as follows Figure 8 As shown. Fig. 9 As shown, a camera is used to photograph the bottom plate after placing the tissue, and the resulting image is as shown in FIG. Fig.10 As shown, the shaded area is the tissue covered area. The two images are provided to the terminal device, and the terminal device can determine the transparency of the tissue according to parameters such as the brightness of the through hole covered by the tissue.

[0064] The images taken by the transparency detector before and after the placement of the tissue can be transmitted to the terminal device via USB cable, Bluetooth, etc. The terminal device determines the transparency of the tissue based on parameters such as the brightness of the through holes in the tissue coverage area in the image. The terminal device can also control the movement of the bracket or lifting mechanism based on the image to obtain a higher quality image.

[0065] The terminal device can be a computer, mobile phone or other smart device. In the embodiment of the present application, the transparency detector directly collects the image of the tissue and provides it to the terminal device for analysis. This process does not require manual observation to determine the transparency, which can reduce the error caused by subjective factors, improve the accuracy of transparency detection, and improve the detection efficiency. At the same time, because the transparency detector uses a smaller through-hole for light transmission, the analyzed tissue unit is smaller, which can improve the accuracy of the detection.

[0066] Furthermore, although exemplary embodiments have been described herein, the scope thereof includes any and all embodiments based on the present application having equivalent elements, modifications, omissions, combinations (eg, schemes that cross various embodiments), adaptations, or changes.

[0067] The elements in the claims are to be interpreted broadly based on the language employed in the claims and are not limited to the examples described in this specification or during the prosecution of the application, which examples are to be interpreted as non-exclusive. Therefore, this specification and examples are intended to be considered as examples only, with the true scope and spirit being indicated by the claims and the full scope of their equivalents.

[0068] The order of each step in the present application is merely exemplary and not restrictive. Without affecting the implementation of the present application (without destroying the required logical relationship between the steps), the execution order of the steps can be adjusted, and the various embodiments obtained after the adjustment still fall within the scope of the present application.

[0069] The above description is intended to be illustrative rather than restrictive. For example, the above examples (or one or more of them) can be used in combination with each other. For example, those of ordinary skill in the art can use other embodiments when reading the above description. In addition, in the above-mentioned specific embodiments, various features can be grouped together to simplify the application. This should not be interpreted as an intention that a disclosed feature that does not require protection is necessary for any claim. On the contrary, the subject matter of the present invention may be less than all the features of a specific disclosed embodiment. Thus, the claims are incorporated into the specific embodiments as examples or embodiments, wherein each claim is independently used as a separate embodiment, and it is considered that these embodiments can be combined with each other in various combinations or arrangements. The scope of the present invention should be determined with reference to the attached claims and the full scope of equivalent forms granted by these claims.

Claims

1. A transparency detector, characterized in that: include: Bottom transparent plate, sample imaging stage, bottom surface light source, camera and bracket; The bottom surface light source is placed between the sample imaging platform and the bottom transparent plate, and the camera is connected to the bracket; The bottom transparent plate comprises: a light-transmitting layer and a light-shielding layer arranged on the light-transmitting layer; The light shielding layer has through holes arranged in rows and columns, and transverse scales and longitudinal scales perpendicular to each other, a line connecting the center points of the through holes in each row is parallel to the transverse scale, a line connecting the center points of the through holes in each column is parallel to the longitudinal scale, the transverse scale and the longitudinal scale are both light-transmissive, the aperture of the through holes is less than 1 mm, and the spacing between the through holes is not more than 10 mm; The sample imaging platform is used to carry the bottom surface light source and the bottom transparent plate; The light emitted by the bottom surface light source passes through the light-transmitting layer and the through hole and irradiates the tissue placed on the bottom transparent plate; The camera is used to take pictures of the tissue placed on the bottom transparent plate, and the taken images are provided to the terminal device so that the terminal device determines the transparency of the tissue based on the parameters of the through holes in the image.

2. The transparency tester according to claim 1, characterized in that: The bracket comprises a connecting block, a lateral moving mechanism and a longitudinal moving mechanism, and the lateral moving mechanism can move on the longitudinal moving mechanism; The camera is fixed on the connecting block, and the connecting block can move on the transverse moving mechanism, and the moving direction of the connecting block is perpendicular to the moving direction of the transverse moving mechanism on the longitudinal moving mechanism.

3. The transparency tester according to claim 1, characterized in that: Further including: Top surface light source; The top surface light source is connected to the bracket and is used to provide supplementary light for the tissue.

4. The transparency tester according to claim 1, characterized in that: Further including: Lifting mechanism; The sample imaging platform is connected to the lifting mechanism, and the sample imaging platform is raised or lowered by the lifting mechanism.

5. The transparency tester according to claim 1, characterized in that: Further comprising: a light shielding shell; Wherein, the bottom transparent plate, the sample imaging platform, the bottom surface light source, the camera and the bracket are located in the light-shielding housing.

6. The transparency tester according to claim 1, characterized in that: The spacing between the through holes is [20 μm-10 mm].

7. The transparency tester according to claim 1, characterized in that: The aperture of the through hole is [2 μm-1 mm].

8. The transparency tester according to claim 1, characterized in that: The light-transmitting layer is any one of quartz glass, optical glass and polymer transparent material.

9. The transparency tester according to claim 1, characterized in that: The light shielding layer is a chrome-plated layer.

10. A transparency detection system, characterized in that: include: A terminal device and a transparency detector as described in any one of claims 1 to 9; The terminal device is used to determine the transparency of the tissue based on the parameters of the through hole in the image taken by the camera.

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