A roselle oil composition and its preparation method and application

Abstract

The present application relates to the field of cosmetics, and specifically discloses a Roselle oil composition, a preparation method thereof, and an application thereof. A Roselle oil composition includes 90-100 parts of sunflower seed oil and 1-5 parts of Roselle extract by weight, and the Roselle extract is processed by microencapsulation; its preparation method is: slightly heating the sunflower seed oil between 35-40°C, adding the Roselle extract to the sunflower seed oil, continuously stirring, and stirring to obtain a Roselle oil composition. A Roselle oil composition of the present application can be applied to skin care products, which has the advantage of applying the efficacy of Roselle to skin care products to meet the requirements of today's consumers for the safety and efficacy of skin care products.

Description

Technical Field

[0001] The present application relates to the field of cosmetics, and more specifically, to a roselle oil composition and a preparation method and application thereof. Background Art

[0002] Roselle, scientifically known as Roselle, is an annual herbaceous plant of the genus Hibiscus in the Malvaceae family. With its unique nutritional value and bioactive ingredients, it has attracted extensive attention and research in recent years. Roselle has not only been planted on a large scale in Guangxi and Yunnan, but its economic value and social benefits have also become increasingly prominent, especially in the field of food and drug development, showing great potential.

[0003] Roselle is rich in high-quality protein, multiple vitamins (such as vitamin C, vitamin A, etc.), minerals (potassium, calcium, magnesium, etc.) and cellulose, which are essential nutrients for maintaining human health. More notably, Roselle is also rich in polyphenols, polysaccharides, organic acids and other bioactive substances, which not only give Roselle powerful antioxidant, antibacterial and anti-inflammatory capabilities, but also lay a solid foundation for its wide application in the fields of medicine and beauty.

[0004] In terms of health care, Roselle has been proven to have a variety of health functions, such as regulating blood pressure, lowering cholesterol, and promoting digestion, due to its various bioactive ingredients. At the same time, Roselle extracts are widely used in the food industry, such as Roselle beverages and Roselle cakes, which not only enrich the variety of food, but also enhance the nutritional value and health benefits of food. In addition, the natural pigments in Roselle are used for food freshness monitoring due to their good stability and high safety, providing a new means for food safety assurance.

[0005] As consumers' requirements for the safety and efficacy of skin care products increase, natural plant extracts are increasingly used in skin care products. As a high-quality plant that is both a medicine and a food, Roselle's rich nutrients and bioactive substances provide new ideas for the development of skin care products. However, there are no research reports on its application in skin care products. Summary of the invention

[0006] In order to apply the efficacy of Roselle to skin care products to meet the requirements of today's consumers for the safety and efficacy of skin care products, the present application provides a Roselle oil composition, a preparation method and application thereof.

[0007] The present application provides a Roselle oil composition using the following technical solution:

[0008] A roselle oil composition comprises 90-100 parts of sunflower seed oil and 1-5 parts of roselle extract by weight, wherein the roselle extract is processed by microencapsulation.

[0009] By adopting the above technical scheme, sunflower seed oil is rich in essential fatty acids, vitamin E, sterols and other ingredients, and has a good moisturizing effect. As a component of the skin barrier, linoleic acid helps to repair and maintain the skin barrier function and reduce transepidermal water loss. Vitamin E can lock in moisture, form a protective film, and help the skin stay hydrated for a long time. Roselle is rich in plant active ingredients such as polyphenols and flavonoids, which have soothing and anti-inflammatory effects, and can effectively reduce skin redness, sensitivity and discomfort, thereby achieving the effect of soothing the skin. Roselle extracts such as proanthocyanidins and ursolic acid have the ability to inhibit inflammatory factors and can reduce the inflammatory response of the skin. These ingredients achieve the effect of inhibiting inflammation by reducing the release of proinflammatory factors (such as IL-1 and TNF-α). Roselle extracts such as anthocyanidins and quercetin have strong antioxidant capacity, which can scavenge free radicals, reduce oxidative stress, prevent skin cell damage, delay skin aging, and protect the skin from environmental pollution and ultraviolet damage. The complex consists of sunflower seed oil and roselle extract, both of which are pure natural ingredients without the addition of synthetic chemicals, thus ensuring the safety and mildness of the product. The combination of natural ingredients can meet the needs of modern consumers for natural, safe and healthy cosmetics.

[0010] Optionally, the method of microencapsulating the Hibiscus sabdariffa extract is as follows:

[0011] The roselle is rapidly frozen and dried using liquid nitrogen, and the dried roselle is crushed into fine powder with a particle size of ≤100 μm;

[0012] The roselle powder is extracted by supercritical carbon dioxide, and 10%-15% ethanol is added as a co-solvent, and the extraction time is 60-90 minutes to obtain an extract;

[0013] The extract and the embedding material are dissolved in ethanol to form an initial mixed solution, wherein the embedding material is selected from sodium alginate or whey protein, and ultrasonic emulsification is performed at a frequency of 20-25kHz and a power of 140-160W for 5-10 minutes to form a uniform microemulsion;

[0014] The microemulsion is spray-dried to form microcapsule particles to obtain the Roselle extract.

[0015] By adopting the above technical scheme, the roselle is quickly frozen and dried with liquid nitrogen before extraction, avoiding the damage of high temperature to heat-sensitive active substances in the traditional heat drying process, thereby ensuring that the extract contains a high concentration of natural antioxidant components. Extraction using the supercritical state of carbon dioxide can ensure the integrity and activity of antioxidant active substances such as polyphenols, anthocyanins, and quercetin in the extract. Adding 10%-15% ethanol as a synergistic solvent further improves the extraction efficiency and increases the dissolution amount of polar compounds. The extract and the embedding material are formed into a uniform microemulsion through ultrasonic emulsification technology, and then spray-dried to form microcapsule particles. This embedding technology can protect the active ingredients from being easily oxidized or degraded, extend the shelf life of the product, and improve its stability and permeability in the skin. The embedding material can also form a moisturizing film on the skin surface, further enhancing the moisturizing effect of the product. The uniform microemulsion formed by ultrasonic emulsification can ensure that the roselle extract is evenly dispersed in the embedding material, thereby improving the release effect of the active ingredient during use.

[0016] Optionally, apple stem cell extract is also included, and the mass ratio of the apple stem cell extract to the roselle extract is (0.4-0.6):1.

[0017] Optionally, the preparation method of the apple stem cell extract is:

[0018] Cut the apple skin into small pieces and immerse them in a mixture of cellulase and pectinase, with the cellulase concentration being 0.5%-1.0% (w / v) and the pectinase concentration being 0.2%-0.5% (w / v). The temperature is controlled at 23-28°C. After completion, the separated cells are transferred to the culture medium.

[0019] Cultivate at a temperature of 22-25°C. After the stem cell population is formed, use an enzymatic extraction method, select 0.1%-0.2% (w / v) esterase, 0.2%-0.5% (w / v) xylanase, 0.1%-0.2% (w / v) protease, the temperature is 4-10°C, and the treatment time is 24-36h;

[0020] After enzymatic extraction, an ultrafiltration membrane with a pore size of 10-50 nm is selected to filter the obtained extract, and the concentrated apple stem cell extract is mixed with the nanoliposomes by high-speed stirring to form a uniform nanoparticle suspension;

[0021] The nanoparticle suspension is subjected to electrostatic spray drying, and the dried powder is redispersed in liposomes to obtain the apple stem cell extract.

[0022] By adopting the above technical solutions, apple stem cells are rich in plant polyphenols, antioxidants and other active ingredients, which can effectively neutralize free radicals and reduce signs of skin aging. At the same time, its ability to promote cell regeneration helps repair damaged skin and improve skin elasticity and firmness. Combined with roselle extract, the synergistic effect of the two further enhances the anti-aging effect of skin care products and improves skin tone and texture. The entire extraction process uses natural enzymatic technology and low-temperature treatment to reduce the damage of chemical solvents and high temperatures to active ingredients, ensuring the safety and mildness of the product. The extracted apple stem cell extract is mixed with nanoliposomes to form a nanoparticle suspension, which can not only protect the stem cell components from the influence of the external environment (such as oxidation, light and temperature changes), but also improve the absorbability of the product on the skin. Nanoliposomes have a small particle size and can better penetrate into the deep layers of the skin. Liposome encapsulation technology can not only improve the penetration of active ingredients, but also avoid the loss or oxidation of active ingredients, extending the shelf life and use effect of skin care products.

[0023] Optionally, jojoba oil and avocado oil are also included, and the mass ratio of the jojoba oil, avocado oil and Roselle extract is (2-3):(1-1.5):1.

[0024] By adopting the above technical solutions, jojoba oil quickly penetrates, providing immediate moisturizing and regulating sebum. Avocado oil acts on deeper skin tissue, nourishing it for a long time and repairing dry and damaged skin barriers. Roselle extract relieves redness and irritation caused by weakened skin barrier function through anti-inflammatory effects, promoting barrier repair from the root. This combination not only provides long-lasting moisturizing effects, but also helps the skin recover from damage caused by environmental stress (such as pollution and ultraviolet rays) and reduces the burden on the skin.

[0025] Roselle extract can remove free radical damage caused by environmental pollution and ultraviolet rays, while the deep nourishment and anti-inflammatory effects of avocado oil can further relieve the inflammatory response caused by oxidative stress. The phytosterols in avocado oil work together with the anti-inflammatory polyphenols (such as ursolic acid) in Roselle extract to reduce the release of pro-inflammatory factors (such as TNF-α and IL-1), reduce skin inflammation, make the skin healthier, and reduce irritation and allergies.

[0026] Jojoba oil has a structure close to the skin's natural oils. It can help regulate the skin's oil secretion and avoid excessive oiliness. It also maintains a moderate water-oil balance on the skin, making it very suitable for oily or combination skin. For oily and combination skin, jojoba oil regulates oil secretion, helps reduce skin shine and clogged pores, and provides proper moisturizing. Roselle extract can reduce sebum secretion while controlling inflammation, acne and other problems common to oily skin through anti-inflammatory mechanisms. The long-lasting nourishing effect of avocado oil ensures that the skin does not become dry due to excessive oil control, avoiding water-oil imbalance.

[0027] Optionally, astaxanthin is also included, and the mass ratio of astaxanthin to Roselle extract is (0.1-0.3):1.

[0028] By adopting the above technical solution, astaxanthin can effectively neutralize free radicals in the skin, prevent skin cell damage caused by oxidative stress, and protect skin cells from damage by external factors such as ultraviolet rays and pollution, thereby delaying skin aging. The polyphenols and flavonoids (such as anthocyanins and quercetin) in Roselle extract already have strong antioxidant capacity. When combined with astaxanthin, the two can synergistically enhance the antioxidant effect, effectively reduce the formation of wrinkles and fine lines, and improve skin elasticity and firmness. Astaxanthin not only has an antioxidant effect, but also has a certain anti-inflammatory effect. The synergistic effect with Roselle extract and jojoba oil significantly enhances the anti-inflammatory and soothing effect of the composition.

[0029] In a second aspect, the present application provides a method for preparing a Roselle oil composition, using the following technical solution:

[0030] A method for preparing a Roselle oil composition comprises the following steps:

[0031] The sunflower seed oil is slightly heated at 35-40° C., and the roselle extract is added into the sunflower seed oil. The mixture is continuously stirred and stirred evenly to obtain a roselle oil composition.

[0032] Optionally, include the following steps:

[0033] Slightly heat the sunflower seed oil between 35-40°C, add the Roselle extract to the sunflower seed oil and keep stirring, add jojoba oil and avocado oil to the mixture in turn, stir at a low speed for 5-10 minutes, then add astaxanthin to the sunflower seed oil while keeping the temperature at 25-30°C, stir at a low speed for 5-10 minutes, then add the apple stem cell extract, stir at a low speed at 20-25°C for 5-10 minutes, to obtain a Roselle oil composition.

[0034] By adopting the above technical scheme, the Roselle oil composition has significant antioxidant, anti-aging, repair and moisturizing effects through the synergistic effect of multiple natural active ingredients, and through scientific temperature control and stirring processes, the activity of the ingredients and the stability of the product are guaranteed, making it suitable for use in high-efficiency skin care products.

[0035] In a third aspect, the present application provides an application of a Roselle oil composition, using the following technical solution:

[0036] An application of a Roselle oil composition, wherein the Roselle oil composition is applied in skin care products.

[0037] In summary, this application has the following beneficial effects:

[0038] 1. Since the composite of the present application includes roselle extract and sunflower seed oil, the combination of natural ingredients improves the safety and effectiveness of the product, while broadening the application scope of the product, meeting consumers' demand for natural and safe cosmetics, and synergizing the soothing and antioxidant effects of roselle extract to achieve long-lasting moisturizing and soothing skin, inhibiting inflammatory factors, and inhibiting oxidative stress. The composite is pure natural, safe, has good soothing effect, and has certain moisturizing and antioxidant effects.

[0039] 2. In this application, the jojoba oil, avocado oil and roselle extract ratio of (2-3): (1-1.5): 1 is preferably used. The three work synergistically to provide a comprehensive skin care effect. Jojoba oil quickly penetrates the skin, providing immediate moisturizing and sebum regulation; avocado oil deeply nourishes and repairs dry and damaged skin barriers; roselle extract relieves damage caused by inflammation and environmental stress through anti-inflammatory and antioxidant mechanisms, helping the skin to recover health. This formula is particularly suitable for regulating oil secretion, balancing water and oil, and ensuring long-term nourishment and repair of the skin. It is suitable for oily and combination skin.

[0040] 3. This application ensures the integrity and efficiency of the active ingredients in the roselle extract and apple stem cell extract through innovative extraction processes. The roselle extract effectively retains its antioxidant and soothing properties through liquid nitrogen quick freezing, carbon dioxide supercritical extraction and ultrasonic emulsification embedding technology, while enhancing its stability and permeability in the skin. Apple stem cell extract uses natural enzymatic and low-temperature treatment technology, combined with nanoliposome encapsulation, which not only retains rich anti-aging ingredients, but also improves its absorption and effect deep in the skin. DETAILED DESCRIPTION

[0041] The present application is further described in detail below in conjunction with the examples. It is particularly noted that if no specific conditions are specified in the following examples, the experiments are carried out according to conventional conditions or the conditions recommended by the manufacturer. Unless otherwise specified, the raw materials used in the following examples can be obtained from common commercial sources.

[0042] Preparation Example 1

[0043] A preparation method of Roselle extract:

[0044] The fresh Roselle is quickly frozen in liquid nitrogen, and the freeze-drying technology is used to remove the moisture in the Roselle to obtain the dried Roselle.

[0045] The dried Roselle is crushed by a crusher until the particle size reaches fine powder of ≤100 μm.

[0046] 10 kg of Roselle powder was placed in a carbon dioxide supercritical extraction device, low-temperature extraction was performed at 35°C, the pressure was controlled at 22 MPa, the CO2 flow rate was maintained at 12±1 L / min, 12% ethanol was added as a co-solvent, the extraction time was 75 minutes, and an extract was obtained.

[0047] The extract and sodium alginate were dissolved in ethanol to form an initial mixed solution, the amount of ethanol used was 3 times the mass of the extract, and the concentration of sodium alginate in the mixed solution was 3% (w / v). The initial mixed solution was treated with ultrasonic emulsification technology at a frequency of 23kHz, a power of 150W, and a treatment time of 8 minutes to form a stable microemulsion.

[0048] The microemulsion obtained above was spray dried using a centrifugal nozzle, the atomization pressure was controlled at 18 MPa, the air flow rate was 40 L / min, the inlet air temperature was set at 110-120°C, the outlet air temperature was set at 60-70°C, and after drying, it was cooled to room temperature. The formed microcapsule particles were collected by a cyclone separator to obtain the Roselle extract.

[0049] Preparation Example 2

[0050] Preparation of a Roselle Extract: The difference from Preparation Example 1 is that whey protein is used as the embedding material, and the concentration of whey protein in the mixed solution is 8% (w / v).

[0051] Preparation Example 3

[0052] Preparation of a Roselle Extract: The difference from Preparation Example 1 is that ethanol is not added as a co-solvent.

[0053] Preparation Example 4

[0054] A preparation method of Roselle extract:

[0055] The fresh Roselle is quickly frozen in liquid nitrogen, and the freeze-drying technology is used to remove the moisture in the Roselle to obtain the dried Roselle.

[0056] The dried Roselle is crushed by a crusher until the particle size reaches fine powder of ≤100 μm.

[0057] 10 kg of Roselle powder was placed in a carbon dioxide supercritical extraction device, low-temperature extraction was performed at 35°C, the pressure was controlled at 22 MPa, the CO2 flow rate was maintained at 12±1 L / min, 12% ethanol was added as a co-solvent, the extraction time was 75 minutes, and an extract was obtained.

[0058] The extract was filtered through a 30 kDa ultrafiltration membrane to obtain the Roselle extract.

[0059] Preparation Example 5

[0060] Preparation of an apple stem cell extract:

[0061] Select fresh, undamaged Utweiler late-ripening apples, wash them and remove the flesh using sterile techniques, leaving only the apple skin. Cut the apple skin into small pieces, with the size of the pieces controlled to about 1 cm³.

[0062] A mixture containing 0.8±0.1% (w / v) cellulase, 0.35±0.1% (w / v) pectinase and 10 mM citric acid-sodium citrate buffer was selected, and the rest was deionized water.

[0063] The temperature of the mixture was controlled at 25°C, and small pieces of apple skin were soaked in the enzyme solution and allowed to stand for 3 hours. After completion, the mixture was centrifuged (5000 rpm, 10 minutes) to remove cell debris and residues, and the apple skin cells were separated and rinsed with sterile water several times to remove residual enzymes and impurities.

[0064] MS culture medium was used, and Yishen Biotechnology 61005ES plant gel (plant cell culture grade) was selected in the culture medium. The culture medium was sterilized in an autoclave at 121°C for 15 minutes. The separated apple cells were transferred to the culture medium and cultured statically at 22-25°C. The suspension culture method was used, and the cells were placed on a shaker with slight stirring to promote cell proliferation and group formation. After 7 days of culture, the apple cells gradually formed cell clusters and generated stem cell populations.

[0065] The enzymatic solution components are as follows: esterase 0.15±0.05% (w / v), xylanase 0.35±0.05% (w / v), protease 0.15±0.05% (w / v), and the remaining components are deionized water. The stem cell population is placed in the enzymatic solution and the enzymatic reaction is carried out for 30 hours in an environment of 6±1°C.

[0066] After the enzymatic extraction is completed, the cell debris and other insoluble substances are separated by centrifugation (8000 rpm, 15 minutes) to obtain a clear extract containing the active ingredients in apple stem cells. An ultrafiltration membrane with a pore size of 30nm is selected to maintain an operating pressure of 0.2MPa to filter the enzymatic solution containing stem cells.

[0067] The ultrafiltration product was mixed with 10% (w / v) nanophosphatidylcholine solution at a mass ratio of 1:3 and stirred at a high speed of 3000 r / min to form a uniform nanoparticle suspension.

[0068] The nanoparticle suspension is treated by an electrostatic spray drying device, and the spray drying temperature is controlled between 40°C. The dried nanoparticle powder is redispersed in a lecithin solution, and the mass ratio of the nanoparticle powder to the lecithin solution is 1:2 to form a uniform nanoliposome suspension to obtain apple stem cell extract.

[0069] Example 1

[0070] A preparation method of a Roselle oil composition:

[0071] Take 9kg of sunflower seed oil (model: Yuanye S24927) and pour it into a container, and slowly heat it to 38°C. During the heating process, stir slowly at a speed of 50r / min to ensure that the oil is heated evenly.

[0072] After the sunflower seed oil was heated to the target temperature, 0.1 kg of Roselle extract was added. The Roselle extract was prepared in Preparation Example 1.

[0073] Continue to stir slowly to ensure that the Roselle extract is evenly dispersed in the oil phase. The stirring time is controlled within 10 minutes to obtain a Roselle oil composition.

[0074] Example 2

[0075] A preparation method of a Roselle oil composition:

[0076] Pour 10kg of sunflower seed oil (model: Yuanye S24927) into a container and slowly heat it to 38°C. During the heating process, stir slowly at a speed of 50r / min to ensure that the oil is heated evenly.

[0077] After the sunflower seed oil was heated to the target temperature, 0.5 kg of Roselle extract was added. The Roselle extract was prepared in Preparation Example 1.

[0078] Continue to stir slowly to ensure that the Roselle extract is evenly dispersed in the oil phase. The stirring time is controlled within 10 minutes to obtain a Roselle oil composition.

[0079] Example 3

[0080] A preparation method of a Roselle oil composition:

[0081] Pour 9.5 kg of sunflower seed oil (model: Yuanye S24927) into a container and slowly heat it to 38°C. During the heating process, stir slowly at a speed of 50 r / min to ensure that the oil is heated evenly.

[0082] After the sunflower seed oil was heated to the target temperature, 0.3 kg of Roselle extract was added. The Roselle extract was prepared in Preparation Example 1.

[0083] Continue to stir slowly to ensure that the Roselle extract is evenly dispersed in the oil phase. The stirring time is controlled within 10 minutes to obtain a Roselle oil composition.

[0084] Example 4

[0085] A preparation method of a Roselle oil composition:

[0086] Pour 9.5 kg of sunflower seed oil (model: Yuanye S24927) into a container and slowly heat it to 38°C. During the heating process, keep stirring slowly to ensure that the oil is heated evenly.

[0087] After the sunflower seed oil was heated to the target temperature, 0.3 kg of Roselle extract was added. The Roselle extract was prepared in Preparation Example 1.

[0088] The mixture was further cooled to 25°C, and then 0.15 kg of apple stem cell extract was added. The apple stem cell extract was prepared in Preparation Example 5, and the mixture was stirred at a low speed of 50 r / min for 10 minutes to obtain a Roselle oil composition.

[0089] Example 5

[0090] A method for preparing a Roselle oil composition: The method is different from Example 4 in that 0.12 kg of apple stem cell extract is added.

[0091] Example 6

[0092] A method for preparing a Roselle oil composition: the method is different from Example 4 in that 0.18 kg of apple stem cell extract is added.

[0093] Example 7

[0094] A preparation method of a Roselle oil composition:

[0095] Pour 9.5 kg of sunflower seed oil (model: Yuanye S24927) into a container and slowly heat it to 38°C. During the heating process, keep stirring slowly to ensure that the oil is heated evenly.

[0096] After the sunflower seed oil was heated to the target temperature, 0.3 kg of Roselle extract was added. The Roselle extract was prepared in Preparation Example 1.

[0097] The mixture was further cooled to 25°C, and then 0.15 kg of apple stem cell extract, 0.75 kg of jojoba oil (purchased from McLean Reagent J885960) and 0.4 kg of avocado oil (purchased from McLean Reagent A790409) were added, the apple stem cell extract was prepared by Preparation Example 5, and stirring was continued at a low speed of 50 r / min for 10 minutes to obtain a Roselle oil composition.

[0098] Example 8

[0099] A method for preparing a Roselle oil composition: the difference from Example 7 is that 0.6 kg of jojoba oil and 0.3 kg of avocado oil are used.

[0100] Example 9

[0101] A method for preparing a Roselle oil composition: the difference from Example 7 is that 0.9 kg of jojoba oil and 0.45 kg of avocado oil are used.

[0102] Example 10

[0103] A preparation method of a Roselle oil composition:

[0104] Pour 9.5 kg of sunflower seed oil (model: Yuanye S24927) into a container and slowly heat it to 38°C. During the heating process, keep stirring slowly to ensure that the oil is heated evenly.

[0105] After the sunflower seed oil was heated to the target temperature, 0.3 kg of Roselle extract was added. The Roselle extract was prepared in Preparation Example 1.

[0106] The mixture was further cooled to 25°C, and then 0.15 kg of apple stem cell extract, 0.75 kg of jojoba oil (purchased from McLean Reagent J885960), 0.4 kg of avocado oil (purchased from McLean Reagent A790409), and 0.06 kg of astaxanthin (purchased from McLean Reagent A832359) were added, and the apple stem cell extract was prepared by Preparation Example 5. The mixture was stirred at a low speed of 50 r / min for 10 minutes to obtain a Roselle oil composition.

[0107] Embodiment 11

[0108] A method for preparing a Roselle oil composition: the difference from Example 10 is that 0.03 kg of astaxanthin is added.

[0109] Example 12

[0110] A method for preparing a Roselle oil composition: the difference from Example 10 is that 0.09 kg of astaxanthin is added.

[0111] Example 13

[0112] A method for preparing a Roselle oil composition: The difference from Example 10 is that the Roselle extract is prepared by Preparation Example 2.

[0113] Comparative Example 1

[0114] A method for preparing a Roselle oil composition: The difference from Example 10 is that the Roselle extract is prepared by Preparation Example 3.

[0115] Comparative Example 2

[0116] A method for preparing a Roselle oil composition: The difference from Example 10 is that the Roselle extract is prepared by Preparation Example 4.

[0117] Comparative Example 3

[0118] A method for preparing a roselle oil composition: The difference from Example 10 is that the apple stem cell extract uses PhytoCellTec™ Malus Domestica apple stem cells from the Swiss Mitsubishi Group.

[0119] Performance testing

[0120] Detection Methods

[0121] (1) Moisturizing effect test:

[0122] 30 people of different genders, ages and skin types were selected, and people with skin diseases, allergies and other history who were not suitable for the test were excluded. The inner side of the human forearm was selected for skin testing, and each tester applied the roselle oil complex of Examples 1-13 and Comparative Examples 1-3 in the same specified area, with the same application area and amount, and the interval between adjacent application areas was 1 cm. A professional skin moisture content measuring instrument Corneometer CM 825 was used, which evaluates the moisturizing state of the skin by measuring the moisture content of the skin stratum corneum, and the data is usually displayed in unit value (AU).

[0123] Before using the product for the first time, measure the baseline moisture value of each tester's test area and record the skin moisture content before using the product. Measure the moisture content 4h, 8h, and 12h after application, record the moisture content value of the test area skin, and calculate its water retention rate.

[0124] Table 1 Moisturizing effect test

[0125]

[0126] (2) DPPH free radical scavenging rate test

[0127] Preparation of DPPH ethanol solution: weigh 20 mg DPPH, dissolve in anhydrous ethanol and dilute to a 250 mL volumetric flask. The DPPH concentration is prepared to be 2 × 10-4 mol / L. Store at 0-4 ° C in the dark and use immediately after preparation. It is effective within 4 hours. (Vitamin C is used as the positive control: the same concentration is used)

[0128] Preparation of the test solution: Prepare four concentrations, dilute 10 times, 20 times, 50 times and 100 times respectively. Take 1.0mL of the test solution and mix it with 1.0mL of 2×10-4mol / L DPPH solution (tube A); take 1.0mL of anhydrous ethanol and mix it with 1.0mL of 2×10-4mol / L DPPH solution (tube B); take 1.0mL of anhydrous ethanol and mix it with 1.0mL of the test solution (tube C); after reacting for 30 minutes, measure the absorbance values ​​of tubes A, B and C at 517nm, and calculate the clearance rate.

[0129] The calculation formula is: Clearance (%) = [(B+C)-A] / B

[0130] (3) HaCaT cell viability test

[0131] HaCaT cells were seeded into 96-well plates at 5,000-10,000 cells per well and cultured for 24 hours to ensure cell attachment.

[0132] The Roselle oil complexes prepared in Examples 1-13 and Comparative Examples 1-3 were used to treat HaCaT cells for 24 hours.

[0133] After the treatment, CCK-8 reagent was added to the cells and incubated for 2 hours. The optical density (OD value) at 450nm was measured by a microplate reader, which is proportional to the cell viability. If the roselle oil complex has no obvious inhibitory effect on cell viability, it indicates that it is safe for HaCaT cells.

[0134] (4) Inflammatory factor testing

[0135] HaCaT cells were cultured in 6-well plates with 1x10^5 cells per well. Cells were treated with the Roselle oil complex for 24 hours.

[0136] After treatment, LPS (1 μg / mL) was added to stimulate the cells and incubated for 24 hours to induce an inflammatory response.

[0137] Total RNA was extracted from cells and reverse transcribed to generate cDNA. qPCR was used to detect the gene expression of inflammatory factor IL-6, and the results were analyzed using the ΔCt method to analyze the relative expression of the gene.

[0138] Collect the cell culture supernatant and use an ELISA kit to detect the IL-6 content in the supernatant. The calculation method is as follows:

[0139] Relative expression of inflammatory factors (%) = inflammatory factor level in treatment group / inflammatory factor level in LPS group*100%

[0140] If the Hibiscus sabdariffa oil complex significantly reduces the levels of LPS-induced inflammatory factors, it indicates that it has an anti-inflammatory effect.

[0141] Table 2 Clearance rate / cell viability / inflammatory factor test data

[0142]

[0143] It can be seen from Example 3 and Comparative Examples 1-2 and Table 1-2 that adding 10%-15% ethanol as a synergistic solvent further improves the extraction efficiency and increases the dissolution amount of polar compounds. The extract and the embedding material are formed into a uniform microemulsion by ultrasonic emulsification technology, and then spray-dried to form microcapsule particles. This embedding technology can protect the active ingredients from being easily oxidized or degraded, extend the shelf life of the product, and improve its stability and permeability in the skin. The embedding material can also form a moisturizing film on the skin surface, further enhancing the moisturizing effect of the product. The uniform microemulsion formed by ultrasonic emulsification can ensure that the Roselle extract is evenly dispersed in the embedding material, thereby improving the release effect of the active ingredient during use.

[0144] Combining Example 4 and Comparative Example 3 with Tables 1-2, it can be seen that the entire extraction process uses natural enzymatic technology and low-temperature treatment, which reduces the damage of chemical solvents and high temperatures to the active ingredients, ensuring the safety and mildness of the product. The extracted apple stem cell extract is mixed with nanoliposomes to form a nanoparticle suspension, which can not only protect the stem cell components from the influence of the external environment, but also improve the absorbency of the product on the skin. Nanoliposomes have a small particle size and can better penetrate into the deep layers of the skin. Liposome encapsulation technology can not only enhance the penetration of active ingredients, but also avoid the loss or oxidation of active ingredients, thereby extending the shelf life and use effect of skin care products.

[0145] From Examples 1-3 and Tables 1-2, it can be seen that the combination of sunflower seed oil and roselle extract effectively achieves soothing and antioxidant effects and has excellent long-lasting moisturizing effects. The ingredients used are all pure natural ingredients without the addition of synthetic chemicals, thereby ensuring the safety and mildness of the product.

[0146] As can be seen from Examples 3-6 and Tables 1-2, apple stem cells are rich in plant polyphenols, antioxidants and other active ingredients, which can effectively neutralize free radicals and reduce signs of skin aging. At the same time, its ability to promote cell regeneration helps repair damaged skin and improve skin elasticity and firmness. When used in combination with Roselle extract, the synergistic effect of the two further enhances the anti-aging effect of skin care products and improves skin tone and texture.

[0147] Combining Example 4 and Example 7-9 with Table 1-2, it can be seen that the jojoba oil, avocado oil and roselle extract ratio of (2-3): (1-1.5): 1 is adopted. The jojoba oil, avocado oil and roselle extract in this formula can not only provide immediate and deep moisturizing, but also have excellent anti-inflammatory and antioxidant effects. Their synergistic effect helps the skin maintain water-oil balance, reduce inflammation, and promote the repair of the skin barrier while resisting pollution, ultraviolet rays and oxidative stress. This combination is very suitable for oily, mixed and sensitive skin, and can comprehensively improve the health of the skin.

[0148] Combining Examples 7 and 10-12 with Tables 1-2, it can be seen that the polyphenols and flavonoids (such as anthocyanins and quercetin) in the roselle extract already have strong antioxidant capacity. When combined with astaxanthin, the two can synergistically enhance the antioxidant effect, effectively reduce the formation of wrinkles and fine lines, and improve skin elasticity and firmness. Astaxanthin not only has an antioxidant effect, but also has a certain anti-inflammatory effect. The synergistic effect with the roselle extract and jojoba oil significantly enhances the anti-inflammatory and soothing effect of the composition.

[0149] It can be seen from Example 10 and Example 13 and Table 1-2 that the embedding material in the present application uses sodium alginate to achieve better results, so that the composition can have better moisturizing and anti-oxidation effects.

[0150] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A Roselle oil composition, characterized in that The components include 90-100 parts of sunflower seed oil, 1-5 parts of roselle extract and apple stem cell extract by weight, wherein the mass ratio of the apple stem cell extract to the roselle extract is (0.4-0.6):1, and the roselle extract is microencapsulated, and the method for microencapsulating the roselle extract is: The roselle is rapidly frozen and dried using liquid nitrogen, and the dried roselle is crushed into fine powder with a particle size of ≤100 μm; The roselle powder is extracted by supercritical carbon dioxide, and 10%-15% ethanol is added as a co-solvent, and the extraction time is 60-90 minutes to obtain an extract; The extract and the embedding material are dissolved in ethanol to form an initial mixed solution, wherein the embedding material is selected from sodium alginate or whey protein, and ultrasonic emulsification is performed at a frequency of 20-25kHz and a power of 140-160W for 5-10 minutes to form a uniform microemulsion; The microemulsion is spray-dried to form microcapsule particles to obtain the Roselle extract; The preparation method of the apple stem cell extract is as follows: Cut the apple skin into small pieces and immerse them in a mixture of cellulase and pectinase, with the cellulase concentration being 0.5%-1.0% (w / v) and the pectinase concentration being 0.2%-0.5% (w / v). The temperature is controlled at 23-28°C. After completion, the separated cells are transferred to the culture medium. Cultivate at a temperature of 22-25°C. After the stem cell population is formed, use an enzymatic extraction method, select 0.1%-0.2% (w / v) esterase, 0.2%-0.5% (w / v) xylanase, 0.1%-0.2% (w / v) protease, the temperature is 4-10°C, and the treatment time is 24-36h; After enzymatic extraction, an ultrafiltration membrane with a pore size of 10-50 nm is selected to filter the obtained extract, and the concentrated apple stem cell extract is mixed with the nanoliposomes by high-speed stirring to form a uniform nanoparticle suspension; The nanoparticle suspension is subjected to electrostatic spray drying, and the dried powder is redispersed in liposomes to obtain the apple stem cell extract.

2. The Roselle oil composition according to claim 1, characterized in that: It also includes jojoba oil and avocado oil, and the mass ratio of the jojoba oil, avocado oil and roselle extract is (2-3):(1-1.5):

1.

3. The Roselle oil composition according to claim 1, characterized in that: It also includes astaxanthin, and the mass ratio of astaxanthin to Roselle extract is (0.1-0.3):

1.

4. A method for preparing the Roselle oil composition according to any one of claims 1 to 3, characterized in that: The following steps are involved: The sunflower seed oil is slightly heated at 35-40° C., and the roselle extract is added into the sunflower seed oil. The mixture is continuously stirred and stirred evenly to obtain a roselle oil composition.

5. The method for preparing the Roselle oil composition according to claim 4, characterized in that: The following steps are involved: Slightly heat the sunflower seed oil between 35-40°C, add the Roselle extract to the sunflower seed oil and keep stirring, add jojoba oil and avocado oil to the mixture in turn, stir at a low speed for 5-10 minutes, then add astaxanthin to the sunflower seed oil while keeping the temperature at 25-30°C, stir at a low speed for 5-10 minutes, then add the apple stem cell extract, stir at a low speed at 20-25°C for 5-10 minutes, to obtain a Roselle oil composition.

6. An application of the Roselle oil composition according to any one of claims 1 to 3, characterized in that: The roselle oil composition is used in skin care products.

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