A composition for preventing and treating calf diarrhea, preparation and preparation method and application thereof

Abstract

The present application relates to the technical field of traditional Chinese medicine, and specifically discloses a composition for preventing and treating calf diarrhea, a preparation method and application thereof. The composition for preventing and treating calf diarrhea provided by the present application selects baicalein, forrestimine and traditional Chinese medicine polysaccharide as active ingredients, and the three can produce a synergistic effect, can significantly enhance the inhibitory effect of the composition on bacteria and viruses; at the same time, can also enhance the immunity of the calf by increasing the content of immunoglobulin and cytokine; in addition, can regulate the intestinal flora of the calf, improve the intestinal flora structure, and promote the intestinal health of the calf, thereby effectively preventing and treating the occurrence of calf diarrhea and promoting the health of the calf. The composition provided by the present application effectively prevents and treats calf diarrhea by selecting raw materials with different mechanisms, is not prone to drug resistance, has stable prevention and treatment effect, and has great popularization value.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of traditional Chinese medicine, and particularly relates to a composition for preventing and treating diarrhea of calf, a preparation method and application thereof. BACKGROUND

[0002] Diarrhea of calf is a high-incidence disease that seriously harms the healthy development of the cattle breeding industry, and can occur throughout the year, especially in early spring, late summer and early autumn. Diarrhea of calf can lead to the overall loss of body fluid and electrolyte, and also reduce the absorption of carbohydrates, lipids and amino acids in the body, so as to reduce the feed reward and immunity, thereby directly affecting the early growth and development of the calf. After the birth of the calf, the gastrointestinal function is not perfect, and the resistance to external stimulation is insufficient, which can easily cause the destruction of the gastrointestinal microorganism homeostasis and lead to severe diarrhea. The clinical symptoms of diarrhea of calf mainly include the following aspects: body temperature rising, about 41 DEG C to 42 DEG C, depression, anorexia, nasal and ocular mucous secretion, nasal mirror and oral mucosa surface erosion, necrosis of the tongue epithelium, increased flow, foul breath, and feces in the form of water with mucus and blood. In addition, respiratory diseases can also be infected during diarrhea. At present, antibiotics and chemical drugs are often used to prevent and treat diarrhea of calf, but long-term use of antibiotics can produce drug resistance and imbalance of intestinal flora, which is not conducive to the growth and development of the calf. Therefore, it is a research hotspot to find a safe and reliable drug that can replace antibiotics to treat diarrhea of calf.

[0003] Traditional Chinese medicine and its active ingredients have the advantages of greenness and safety, can promote the proliferation of beneficial bacteria in the gastrointestinal tract of the calf, inhibit the growth of harmful bacteria, improve the gastrointestinal environment, and improve the digestion and absorption of nutrients in the feed by the calf. However, the existing traditional Chinese medicine preparations for preventing and treating diarrhea of calf often have the problems of large addition amount and unstable use effect, which become the bottleneck of industrialization. SUMMARY

[0004] In view of the problems of large addition amount and unstable use effect of the traditional Chinese medicine preparations for preventing and treating diarrhea of calf in the prior art, the present application provides a composition for preventing and treating diarrhea of calf, a preparation method and application thereof.

[0005] To solve the above technical problems, the technical scheme provided by the present application is as follows:

[0006] In a first aspect, the present application provides a composition for preventing and treating diarrhea of calf, which comprises baicalein, scrophulariamaritin and traditional Chinese medicine polysaccharide; wherein the mass ratio of baicalein to scrophulariamaritin is 5:1 to 15:1, and the mass ratio of the total weight of baicalein and scrophulariamaritin to traditional Chinese medicine polysaccharide is 1:5 to 1:15.

[0007] For the prior art, the composition for preventing and treating calf diarrhea provided by the present application selects baicalein, shahfutaglycoside and traditional Chinese medicine polysaccharide as active ingredients in a specific proportion, and the three can produce a synergistic effect, which can significantly enhance the inhibitory effect of the composition on bacteria and viruses; at the same time, it can also enhance the immunity of the calf by increasing the content of immunoglobulin and cytokine; in addition, it can also regulate the intestinal flora of the calf, improve the intestinal flora structure and promote the intestinal health of the calf, thereby effectively preventing and treating the occurrence of calf diarrhea and promoting the health of the calf. The composition provided by the present application significantly improves the prevention and treatment effect on calf diarrhea by selecting raw materials with different mechanisms, and does not produce drug resistance, has stable prevention and treatment effect and great popularization value.

[0008] Baicalein is a flavonoid component extracted from important scutellaria, which is a broad-spectrum antibacterial drug and has inhibitory effect on gram-positive bacteria and gram-negative bacteria. Its antibacterial mechanism is related to inhibiting the formation of bacterial cell wall and inhibiting bacterial energy metabolism. Baicalein can also play an anti-inflammatory role by inhibiting the release of cytokines that mediate inflammatory reactions. In addition, baicalein also has the effects of improving animal production performance, antioxidant and regulating intestinal microbial balance, and can be used to improve breeding efficiency. However, the antibacterial effect of single baicalein is not ideal, and it cannot effectively prevent the occurrence of calf diarrhea.

[0009] Shahfutaglycoside widely exists in food such as lotus seed core and Chinese herbal medicine, and has the effects of anti-inflammatory and antioxidant. Shahfutaglycoside can significantly reduce the release amount of inflammatory mediator nitric oxide and the expression of tumor necrosis factor, and play an anti-inflammatory role. Shahfutaglycoside also has good antioxidant activity.

[0010] Traditional Chinese medicine polysaccharide has various physiological activities such as antioxidant, intestinal flora regulation, blood glucose reduction and blood lipid reduction. After being applied to a calf, it can effectively improve the antioxidant capacity and immunity of the calf, and promote the growth of beneficial bacteria in the intestinal tract of the calf, reduce the growth of harmful bacteria, and has an important health care effect on calves with underdeveloped intestinal tract and low immunity.

[0011] Further, the mass ratio of baicalein to shahfutaglycoside is 8:1 to 12:1, and the mass ratio of the total weight of baicalein and shahfutaglycoside to traditional Chinese medicine polysaccharide is 1:8 to 1:12.

[0012] Further, the mass ratio of baicalein to shahfutaglycoside is 10:1, and the mass ratio of the total weight of baicalein and shahfutaglycoside to traditional Chinese medicine polysaccharide is 1:10.

[0013] In the preferred mass ratio case, baicalein, shahfutaglycoside and Chinese medicine polysaccharide have very obvious synergistic effect, which can effectively improve the inhibition of the composition on bacteria and viruses, and fully exert the functions of the composition in enhancing immunity, regulating gastrointestinal flora and resisting oxidation, thereby effectively improving the protection rate of the composition on calf diarrhea.

[0014] Preferably, the composition for preventing and treating calf diarrhea comprises the following components in mass fraction: baicalein 5 parts, shahfutaglycoside 0.5 parts and Chinese medicine polysaccharide 55 parts.

[0015] Further, the Chinese medicine polysaccharide is at least one of agaric polysaccharide, astragalus polysaccharide, jujube polysaccharide, lentinan or yam polysaccharide.

[0016] In the second aspect, the application further provides a preparation method of the composition for preventing and treating calf diarrhea, comprising the following steps: weighing each component according to the ratio, mixing the weighed baicalein and shahfutaglycoside uniformly, then adding the weighed Chinese medicine polysaccharide and mixing uniformly to obtain the composition for preventing and treating calf diarrhea.

[0017] In the third aspect, the application further provides a Chinese medicine preparation for preventing and treating calf diarrhea, comprising the composition for preventing and treating calf diarrhea.

[0018] Further, the Chinese medicine preparation for preventing and treating calf diarrhea further comprises a pharmaceutically acceptable excipient.

[0019] As a specific embodiment of the application, the excipient comprises at least one of a solubilizing agent, a dispersing agent, a lubricant, a wetting agent, an antifoaming agent, a disintegrating agent, a thickening agent or a filler.

[0020] In order to prepare the composition of the application into various dosage forms, various excipients need to be added, and the solubilizing agent, the dispersing agent, the lubricant, the wetting agent, the antifoaming agent, the disintegrating agent, the thickening agent or the filler are all common types in the pharmaceutical field. Different excipients can be selected according to the different dosage forms, and the specific components and amounts can be determined according to the formula requirements through conventional experiments.

[0021] Optionally, the dosage form of the Chinese medicine preparation is powder, suspension, water emulsion, microemulsion, emulsion, water dispersible granules or granules.

[0022] The above dosage forms can all be selected from conventional dosage forms in the pharmaceutical field and prepared according to conventional preparation processes. The composition is prepared into various dosage forms for convenient use to meet different medication needs and has a wide range of applications.

[0023] Further, the medication amount of the Chinese medicine preparation is 0.1 g / kg BW-0.5 g / kg BW based on the composition for preventing and treating calf diarrhea.

[0024] Further, the Chinese medicine preparation has a feeding period of 7-30 days.

[0025] In a fourth aspect, the present application also provides the composition or the Chinese medicine preparation as described in any one of the above for use in preventing and treating calf diarrhea.

[0026] It should be noted that the method for using the composition or the Chinese medicine preparation provided by the present application is to directly add the composition or the Chinese medicine preparation into milk replacer and feed together with milk.

[0027] The baicalein, scrophulariamaritin and Chinese medicine polysaccharide are combined in the present application, and the three components synergistically play the effects of antibacterial and antiviral, antioxidation, immune enhancement and improvement of intestinal flora structure of the calf, and have small toxic and side effects and are not easy to produce drug resistance, which is more conducive to the long-term health of the calf and promotes the healthy growth of the calf, and provides a new method for preventing and treating calf diarrhea instead of antibiotics, and is conducive to the sustainable development of the cattle breeding industry. DETAILED DESCRIPTION

[0028] In order to make the object, technical solutions and advantages of the present application clearer, the present application will be further described in detail below with reference to examples. It should be understood that the specific examples described herein are only used to explain the present application, and are not used to limit the present application.

[0029] If a specific technology or condition is not specified in the examples, the technology or condition is carried out according to the technology or condition described in the literature in the art or according to the product instruction. If the reagent or instrument is not specified by the manufacturer, it is a conventional product that can be purchased through a regular channel.

[0030] In order to better illustrate the present application, the following examples are further illustrated.

[0031] Example 1

[0032] The present embodiment provides a composition for preventing and treating calf, which comprises the following components in mass fraction:

[0033] Baicalein 5g, scrophulariamaritin 0.5g and Chinese medicine polysaccharide 55g.

[0034] The preparation method of the composition for preventing and treating calf diarrhea comprises the following steps: weighing each component according to the ratio, mixing the weighed baicalein and scrophulariamaritin uniformly, adding the weighed Chinese medicine polysaccharide, and mixing uniformly to obtain the composition for preventing and treating calf diarrhea.

[0035] The Chinese medicine composition is prepared according to the above formula and method by taking agaricus blazei polysaccharide, astragalus polysaccharide, jujube polysaccharide, lentinan and yam polysaccharide, respectively.

[0036] Example 2

[0037] The embodiment provides a composition for preventing and treating diarrhea of a calf, which comprises the following components in mass fractions:

[0038] Baicalein 5g, shatavari glycoside 1g and agaric polysaccharide 30g.

[0039] The preparation method of the composition for preventing and treating diarrhea of a calf comprises the following steps: weighing the components according to the proportion, uniformly mixing the weighed baicalein and shatavari glycoside, adding the weighed agaric polysaccharide, and uniformly mixing to obtain the composition for preventing and treating diarrhea of a calf.

[0040] Embodiment 3

[0041] The embodiment provides a composition for preventing and treating diarrhea of a calf, which comprises the following components in mass fractions:

[0042] Baicalein 15g, shatavari glycoside 1g and agaric polysaccharide 240g.

[0043] The preparation method of the composition for preventing and treating diarrhea of a calf comprises the following steps: weighing the components according to the proportion, uniformly mixing the weighed baicalein and shatavari glycoside, adding the weighed agaric polysaccharide, and uniformly mixing to obtain the composition for preventing and treating diarrhea of a calf.

[0044] Embodiment 4

[0045] The embodiment provides a composition for preventing and treating diarrhea of a calf, which comprises the following components in mass fractions:

[0046] Baicalein 8g, shatavari glycoside 1g and agaric polysaccharide 72g.

[0047] The preparation method of the composition for preventing and treating diarrhea of a calf comprises the following steps: weighing the components according to the proportion, uniformly mixing the weighed baicalein and shatavari glycoside, adding the weighed agaric polysaccharide, and uniformly mixing to obtain the composition for preventing and treating diarrhea of a calf.

[0048] Comparative Example 1

[0049] The comparative example provides a composition for preventing and treating diarrhea of a calf, which is different from the embodiment 1 in that baicalein is replaced by an equal amount of naringin, and specifically comprises the following components in mass fractions:

[0050] Naringin 5g, shatavari glycoside 0.5g and agaric polysaccharide 55g.

[0051] The composition for preventing and treating diarrhea of a calf is prepared by the same method as in the embodiment 1.

[0052] Comparative Example 2

[0053] The comparative example provides a composition for preventing and treating diarrhea of a calf, which is different from the embodiment 1 in that shatavari glycoside is replaced by an equal amount of daidzein, and specifically comprises the following components in mass fractions:

[0054] Baicalein 5 g, daidzein 0.5 g and agaric polysaccharide 55 g.

[0055] The composition for preventing and treating calf diarrhea was prepared by the same method as in Example 1.

[0056] Effect test

[0057] 1. Mouse experiment

[0058] Ninety healthy adult clean Kunming mice, weighing 18-22 g, were selected and adaptively fed for 7 days, and then randomly divided into 9 groups, 10 mice in each group, half male and half female. The senna water decoction (1 g / mL) was used to establish a mouse diarrhea model. After the animals were grouped, in the morning every day, the control group was given normal saline 0.4 mL per mouse by gavage, and the other 7 groups were given senna water decoction 0.4 mL per mouse by gavage. In the afternoon every day, the control group was given 0.1% carboxymethylcellulose sodium solution 0.4 mL per mouse by gavage, and the other 7 groups were given 0.1% carboxymethylcellulose sodium solution corresponding to the corresponding drugs 0.4 mL per mouse by gavage. The amount of active ingredients of traditional Chinese medicine contained in the corresponding volume of drug solution was: (1) baicalein 100 mg / kg BW, (2) psoralen 10 mg / kg BW, (3) agaric polysaccharide 1100 mg / kg BW, (4) baicalein 100 mg / kg BW + psoralen 10 mg / kg BW, (5) baicalein 100 mg / kg BW + agaric polysaccharide 1100 mg / kg BW, (6) agaric polysaccharide 1100 mg / kg BW + psoralen 10 mg / kg BW, (7) baicalein 100 mg / kg BW + psoralen 10 mg / kg BW + agaric polysaccharide 1100 mg / kg BW.

[0059] The administration was continued for 7 days, and during the administration period, a layer of filter paper was laid under the cage to observe the activity and defecation state and frequency of the mice in each group. The mice were fed individually during the experiment, and the diarrhea rate of the mice was observed and recorded every day. The results are shown in Table 1.

[0060] Table 1 Diarrhea rate of mice in each treatment group (n=10)

[0061]

[0062] The above results show that there is a significant synergistic effect among baicalein, psoralen and agaric polysaccharide, and baicalein + psoralen + agaric polysaccharide has the best protection rate for mouse diarrhea caused by senna water decoction.

[0063] In order to verify the effect of other traditional Chinese medicine polysaccharides, the following experiments were carried out:

[0064] Seventy-two healthy adult clean Kunming mice, weighing 18-22 g, were adaptively fed for 7 days and then randomly divided into 6 groups, 12 mice in each group, half male and half female. The mouse diarrhea model was established by senna leaf decoction (1 g / mL). After the animals were grouped, 0.4 mL of senna leaf decoction was administered to each mouse by gavage every morning, and 0.1% CMC-Na solution of the corresponding medicine was administered to each mouse by gavage every afternoon. The amount of active ingredients of traditional Chinese medicine in the corresponding volume of the medicine solution was 100 mg of baicalein + 10 mg of scophyllan + (1) 1100 mg of agaric polysaccharide (2) 1100 mg of yam polysaccharide (3) 1100 mg of astragalus polysaccharide (4) 1100 mg of jujube polysaccharide (5) 1100 mg of lentinan polysaccharide (6) 1100 mg of agaric polysaccharide + lentinan polysaccharide (mass ratio 1:1), which were administered to the mice by gavage at 1210 mg / kg BW.

[0065] The administration was continuously performed for 7 days. During the administration, a layer of filter paper was placed under the cage to observe the activity and defecation state and frequency of the mice in each group (the mice were individually fed during the experiment). The diarrhea rate of the mice was observed and recorded every day. The results are shown in Table 2.

[0066] Table 2 Diarrhea rate of mice in each treatment group (n=12)

[0067]

[0068] The results show that agaric polysaccharide, yam polysaccharide, astragalus polysaccharide, lentinan polysaccharide or a mixture of two polysaccharides, in combination with baicalein and scophyllan, can improve the diarrhea protection rate of senna leaf decoction in mice.

[0069] According to the same experimental process described above, the compositions prepared in Examples 2-4 were tested. The compositions prepared in Examples 2-4 were administered to the mice at 1210 mg / kg BW, and the results were observed and recorded. The results are shown in Table 3.

[0070] Table 3 Diarrhea rate of mice in each treatment group (n=12)

[0071] Group Cumulative number of diarrheal animals Diarrhea protection rate (%) Example 2 2 83.33％ Example 3 3 75.00％ Example 4 2 83.33％

[0072] The above results prove that baicalein, scophyllan and agaric polysaccharide, in a specific ratio, can improve the prevention and treatment effect on calf diarrhea.

[0073] 2. Practical application

[0074] The experiment selects a pasture in Hebei province, the average incidence of diarrhea of the calf in the pasture is 40%, selects the Holstein diarrhea calf with similar age (30±5) and weight (55.33±1.86) kg, and randomly divides into 4 groups, respectively, the control group, the example 1 group, the comparative example 1 group and the comparative example 2 group, 12 heads in each group. During the experiment, all the calves are fed according to the conventional feeding management mode of the pasture, 5L milk replacer is fed daily, and the open feed is put after feeding for the calf to freely eat. The example 1, comparative example 1-2 groups are fed milk replacer (the formula is shown in table 6) and open feed (the formula is shown in table 5), and 10g / head of the composition of the example 1 and comparative example 1-2 groups is added in the milk replacer in the morning and evening, continuously added for 10 days, and the control group is only fed milk replacer without adding drug ingredients. During the experiment, the diarrhea times of the calf are observed and recorded, and the diarrhea is recorded according to the Bristol stool type. On the 11th day of the experiment, 5mL of blood is collected from the calf vein in the morning before feeding for blood immune index and IL-2 determination, so as to evaluate the influence of the composition on the immune function of the calf, and the experimental results are shown in table 4 and table 5.

[0075] Table 4 Influence of the composition on the immune level and inflammatory factor of the calf

[0076]

[0077]

[0078] Note: In the same column data, * indicates significant difference (P<0.05) compared with the control group.

[0079] Table 4 Cure rate of the composition on the diarrhea of the calf

[0080]

[0081] The results show that the composition prepared by the example 1 added in the milk replacer can effectively improve the overall immune level of the calf, and effectively reduce the diarrhea rate of the calf. The composition of the comparative example 1 added in the milk replacer cannot effectively improve the overall immune level of the calf, but can improve the content of IL-2 in the blood, and also cannot effectively reduce the diarrhea rate of the calf. The composition of the comparative example 2 added in the milk replacer has the effect of improving IgA, IgG and IgM in the serum of the calf, but there is no significant difference, and has the effect of partially relieving the diarrhea of the calf, but the effect is not as good as the example 1.

[0082] Table 5 Nutritional level of open feed

[0083]

[0084]

[0085] Note: 1) Premix provides per kg of feed: VA 9 000 IU, VD3 3 000 IU, VK3 2.5 mg, VB1 3 mg, VB2 5 mg, VB5 20 mg, VB 12 0.02 mg, VE 35 IU, biotin 0.12 mg, niacin 20 mg, Cu 8 mg, Zn 75 mg, Fe 80 mg, Mn 100 mg, Se 0.15 mg, I 0.35 mg.

[0086] 2) Metabolic energy is calculated, the rest is measured.

[0087] Table 6 Nutritional levels of milk replacer

[0088]

[0089]

[0090] The above description is only the preferred embodiment of the present application, and is not used to limit the present application, any modification, equivalent replacement or improvement within the spirit and principle of the present application should be included in the protection scope of the present application.

Claims

1. A composition for the control of calf scours, characterized by, Baicalein, shahfutaglycoside and traditional Chinese medicine polysaccharide; wherein the mass ratio of baicalein to shahfutaglycoside is 5:1-15:1, and the mass ratio of the total weight of baicalein and shahfutaglycoside to traditional Chinese medicine polysaccharide is 1:5-1:

15.

2. The composition for preventing and treating calf diarrhea according to claim 1, wherein The mass ratio of baicalein to shahfutaglycoside is 8:1-12:1, and the mass ratio of the total weight of baicalein and shahfutaglycoside to traditional Chinese medicine polysaccharide is 1:8-1:

12.

3. The composition for preventing and treating calf diarrhea as described in claim 2, characterized in that, The mass ratio of baicalein to shahfutaglycoside is 10:1, and the mass ratio of the total weight of baicalein and shahfutaglycoside to traditional Chinese medicine polysaccharide is 1:

10.

4. The composition for preventing and treating calf diarrhea according to claim 3, wherein the composition is administered to the calf in an amount of 0.1 to 1.0 g per 1 kg of body weight of the calf. The composition comprises the following components by mass: baicalein 5 parts, shahfutaglycoside 0.5 parts and traditional Chinese medicine polysaccharide 55 parts.

5. The composition for preventing and treating calf diarrhea according to any one of claims 1 to 4, wherein The traditional Chinese medicine polysaccharide is at least one of agaric polysaccharide, astragalus polysaccharide, jujube polysaccharide, lentinan or yam polysaccharide.

6. A method for preparing the composition for preventing and treating calf diarrhea according to any one of claims 1 to 5, characterized by, The method comprises the following steps: weighing each component according to the ratio, mixing the weighed baicalein and shahfutaglycoside uniformly, adding the weighed traditional Chinese medicine polysaccharide, and mixing uniformly to obtain the composition for preventing and treating calf diarrhea.

7. A Chinese medicine preparation for preventing and treating calf diarrhea, characterized in that, The composition for preventing and treating calf diarrhea is prepared by the method.

8. The traditional Chinese medicine preparation for preventing and treating calf diarrhea as described in claim 7, characterized in that, The dosage form of the traditional Chinese medicine preparation is powder, suspension, water emulsion, microemulsion, emulsifiable concentrate, water dispersible granule or granule.

9. The traditional Chinese medicine preparation for preventing and treating calf diarrhea as described in claim 7, characterized in that, The dosage of the traditional Chinese medicine preparation is 0.1 g / kg BW-0.5 g / kg BW based on the composition for preventing and treating calf diarrhea.

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