Efficient ammonia nitrogen degrading bacterium and application thereof in aquaculture

Abstract

The invention discloses an efficient ammonia nitrogen degrading bacterium which comprises the following raw materials in parts by weight. The microbial agent is prepared from 10-15 parts of ammonia oxidizing bacteria, 10-15 parts of whole-course nitrifying bacteria, 5-8 parts of acinetobacter calcoaceticus, 2-5 parts of bacillus licheniformis, 2-5 parts of denitrifying bacteria, 2-5 parts of compound enzymes and 5-8 parts of nutritional agents. According to the efficient ammonia nitrogen degrading bacteria, ammonia oxidizing bacteria, whole-course nitrifying bacteria and other raw materials are adopted for cooperation, compound enzymes and nutritional agents are added to serve as functional auxiliaries, meanwhile, additives are added to blend and cooperate with the degrading bacteria, and an anaerobic bacterium agent can make full use of an N source, a C source, a P source and the like in waste water for life activities.

Description

Technical Field

[0001] The invention relates to the technical field of degradation bacteria, and in particular to a high-efficiency ammonia nitrogen-degrading bacterium and an application thereof in aquaculture. Background Art

[0002] The main pollutants in aquaculture water are: organic matter, ammonia nitrogen, nitrite, nitrate, phosphorus, etc. Its main characteristics are: large water volume, fewer types of pollutants and small changes in content. The pollutants are mainly organic matter and nutrients such as nitrogen and phosphorus. Most aquaculture wastewater is slightly polluted water, with relatively low pollution load and relatively easy to treat. Some aquaculture wastewater does not even require physical and chemical treatment, and can be directly treated by biological methods to meet the discharge requirements.

[0003] Existing ammonia nitrogen degrading bacteria are used to improve the ammonia nitrogen removal rate, but the COD removal rate is poor. It is difficult to coordinate the improvement of ammonia nitrogen and COD, which limits the removal rate of ammonia nitrogen degrading bacteria. Summary of the invention

[0004] In view of the defects of the prior art, the object of the present invention is to provide a highly efficient ammonia nitrogen degrading bacteria and its application in aquaculture to solve the problems raised in the above background technology.

[0005] The present invention solves the technical problem by adopting the following technical solution: The present invention provides a highly efficient ammonia nitrogen degrading bacteria, comprising the following raw materials in parts by weight: 10-15 parts of ammonia oxidizing bacteria, 10-15 parts of full-process nitrifying bacteria, 5-8 parts of calcium-acetic acid Acinetobacter, 2-5 parts of Bacillus licheniformis, 2-5 parts of denitrifying bacteria, 2-5 parts of complex enzymes and 5-8 parts of nutrients.

[0006] Preferably, the complex enzyme is a mixture of protease, lipase and amylase in a weight ratio of 1:1:1; the nutrient is prepared by 2-5 parts of peptone, 1-3 parts of beef extract and 2-5 parts of sodium chloride.

[0007] Preferably, the highly efficient ammonia nitrogen degrading bacteria further comprises 2-5 parts of additives.

[0008] Preferably, the preparation method of the additive is: S01: heat-treating the nano-titanium dioxide at 55-60°C for 5-10 minutes, and then immersing it in a sufficient amount of a conditioning agent for ultrasonic modification. After the ultrasonic modification is completed, an additive solution is obtained; S02: Stir and mix the carbon nanotubes in a sufficient amount of hydrogen peroxide solution, then wash and dry them, stir and modify the dried carbon nanotubes and the additive solution in a weight ratio of 2:5, and after stirring, wash and dry them to obtain the additive.

[0009] Preferably, the ultrasonic power of the ultrasonic modification treatment is 450-500W, and the ultrasonic time is 20-30min.

[0010] Preferably, the mass fraction of the hydrogen peroxide solution is 2-5%.

[0011] Preferably, the stirring speed of the stirring modification treatment is 450-550 r / min, and the stirring time is 20-30 min.

[0012] Preferably, the preparation method of the regulator is: 2-5 parts of dodecylbenzenesulfonic acid solution, 1-3 parts of silane coupling agent and 2-4 parts of nano-wollastonite are mixed and added into 4-7 parts of dopamine hydrochloride solution, and the mixture is continued to be fully mixed to obtain a regulator.

[0013] Preferably, the mass fraction of the dopamine hydrochloride solution is 2-5%; the mass fraction of the dodecylbenzenesulfonic acid solution is 3-6%.

[0014] Preferably, the silane coupling agent is silane coupling agent KH560.

[0015] Application of a highly efficient ammonia nitrogen degrading bacterium in aquaculture.

[0016] Compared with the prior art, the present invention has the following beneficial effects: The high-efficiency ammonia nitrogen degrading bacteria of the present invention are formulated with ammonia oxidizing bacteria, full-process nitrifying bacteria and other raw materials, and by adding complex enzymes and nutrients as functional additives, and adding additives to coordinate the degradation bacteria, the anaerobic bacteria agent can make full use of the N source, C source, P source and the like in the wastewater for life activities, and convert NH3-N into N02-, N02-, while consuming a large amount of organic matter, and converting the organic matter into CO2, H2O, 5-8 parts, so that the ammonia nitrogen and COD in the water body can be reduced at the same time. The bacterial agent is enriched with multiple groups of anaerobic and facultative anaerobic nitrifying bacteria and denitrifying bacteria, which can mainly degrade ammonia nitrogen, eliminate the sediment, strengthen the indigenous microbial flora of the sediment, establish a microbial ecological flora, and is used for the treatment of high ammonia nitrogen water bodies and the elimination of black and odorous water sediments. DETAILED DESCRIPTION

[0017] The following is a clear and complete description of the technical solutions in the embodiments of the present invention in conjunction with specific embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0018] The highly efficient ammonia nitrogen degrading bacteria of this embodiment include the following raw materials in parts by weight: 10-15 parts of ammonia oxidizing bacteria, 10-15 parts of full-process nitrifying bacteria, 5-8 parts of calcium-acetic acid Acinetobacter, 2-5 parts of Bacillus licheniformis, 2-5 parts of denitrifying bacteria, 2-5 parts of complex enzymes and 5-8 parts of nutrients.

[0019] The complex enzyme of this embodiment is a mixture of protease, lipase and amylase in a weight ratio of 1:1:1; the nutrient is prepared by 2-5 parts of peptone, 1-3 parts of beef extract and 2-5 parts of sodium chloride.

[0020] The high-efficiency ammonia nitrogen-degrading bacteria of this embodiment also include 2-5 parts of additives.

[0021] The preparation method of the additive of this embodiment is: S01: heat-treating the nano-titanium dioxide at 55-60°C for 5-10 minutes, and then immersing it in a sufficient amount of a conditioning agent for ultrasonic modification. After the ultrasonic modification is completed, an additive solution is obtained; S02: Stir and mix the carbon nanotubes in a sufficient amount of hydrogen peroxide solution, then wash and dry them, stir and modify the dried carbon nanotubes and the additive solution in a weight ratio of 2:5, and after stirring, wash and dry them to obtain the additive.

[0022] The ultrasonic power of the ultrasonic modification treatment in this embodiment is 450-500W, and the ultrasonic time is 20-30min.

[0023] The mass fraction of the hydrogen peroxide solution in this embodiment is 2-5%.

[0024] The stirring speed of the stirring modification treatment in this embodiment is 450-550 r / min, and the stirring time is 20-30 min.

[0025] The preparation method of the regulator of this embodiment is: 2-5 parts of dodecylbenzenesulfonic acid solution, 1-3 parts of silane coupling agent and 2-4 parts of nano-wollastonite are mixed and added into 4-7 parts of dopamine hydrochloride solution, and the mixture is continued to be fully mixed to obtain a regulator.

[0026] The mass fraction of the dopamine hydrochloride solution in this embodiment is 2-5%; the mass fraction of the dodecylbenzenesulfonic acid solution is 3-6%.

[0027] The silane coupling agent of this embodiment is silane coupling agent KH560.

[0028] Example 1. The highly efficient ammonia nitrogen degrading bacteria of this embodiment include the following raw materials in parts by weight: 10 parts of ammonia oxidizing bacteria, 10 parts of full-process nitrifying bacteria, 5 parts of calcium-acetic acid Acinetobacter, 2 parts of Bacillus licheniformis, 2 parts of denitrifying bacteria, 2 parts of complex enzymes and 5 parts of nutrients.

[0029] The complex enzyme of this embodiment is a mixture of protease, lipase and amylase in a weight ratio of 1:1:1; the nutrient is prepared by 2 parts of peptone, 1 part of beef extract and 2 parts of sodium chloride.

[0030] The high-efficiency ammonia nitrogen-degrading bacteria of this embodiment also include 2 portions of additives.

[0031] The preparation method of the additive of this embodiment is: S01: heat-treat the nano-titanium dioxide at 55°C for 5 minutes, and then immerse it in a sufficient amount of a conditioning agent for ultrasonic modification. After the ultrasonic treatment is completed, an additive solution is obtained; S02: Stir and mix the carbon nanotubes in a sufficient amount of hydrogen peroxide solution, then wash and dry them, stir and modify the dried carbon nanotubes and the additive solution in a weight ratio of 2:5, and after stirring, wash and dry them to obtain the additive.

[0032] The ultrasonic power of the ultrasonic modification treatment in this embodiment is 450 W, and the ultrasonic time is 20 min.

[0033] The mass fraction of the hydrogen peroxide solution in this embodiment is 2%.

[0034] The stirring speed of the stirring modification treatment in this embodiment is 450 r / min, and the stirring time is 20 min.

[0035] The preparation method of the regulator of this embodiment is: 2 parts of dodecylbenzenesulfonic acid solution, 1 part of silane coupling agent and 2 parts of nano-wollastonite are blended and added into 4 parts of dopamine hydrochloride solution, and the blending is continued to be fully obtained to obtain a regulator.

[0036] The mass fraction of the dopamine hydrochloride solution in this embodiment is 2%; the mass fraction of the dodecylbenzenesulfonic acid solution is 3%.

[0037] The silane coupling agent of this embodiment is silane coupling agent KH560.

[0038] Example 2. The highly efficient ammonia nitrogen degrading bacteria of this embodiment include the following raw materials in parts by weight: 15 parts of ammonia oxidizing bacteria, 5 parts of complete nitrifying bacteria, 8 parts of calcium-acetic acid Acinetobacter, 5 parts of licheniformis, 5 parts of denitrifying bacteria, 5 parts of complex enzymes and 8 parts of nutrients.

[0039] The complex enzyme of this embodiment is a mixture of protease, lipase and amylase in a weight ratio of 1:1:1; the nutrient is prepared by 5 parts of peptone, 3 parts of beef extract and 5 parts of sodium chloride.

[0040] The high-efficiency ammonia nitrogen-degrading bacteria of this embodiment also include 5 parts of additives.

[0041] The preparation method of the additive of this embodiment is: S01: heat-treating the nano-titanium dioxide at 60°C for 10 minutes, and then immersing it in a sufficient amount of a conditioning agent for ultrasonic modification. After the ultrasonic treatment is completed, an additive solution is obtained; S02: Stir and mix the carbon nanotubes in a sufficient amount of hydrogen peroxide solution, then wash and dry them, stir and modify the dried carbon nanotubes and the additive solution in a weight ratio of 2:5, and after stirring, wash and dry them to obtain the additive.

[0042] The ultrasonic power of the ultrasonic modification treatment in this embodiment is 500 W, and the ultrasonic time is 30 min.

[0043] The mass fraction of the hydrogen peroxide solution in this embodiment is 5%.

[0044] The stirring speed of the stirring modification treatment in this embodiment is 550 r / min, and the stirring time is 30 min.

[0045] The preparation method of the regulator of this embodiment is: 5 parts of dodecylbenzenesulfonic acid solution, 3 parts of silane coupling agent and 4 parts of nano-wollastonite are blended and added into 7 parts of dopamine hydrochloride solution, and the blending is continued to obtain a regulator.

[0046] The mass fraction of the dopamine hydrochloride solution in this embodiment is 5%; the mass fraction of the dodecylbenzenesulfonic acid solution is 6%.

[0047] The silane coupling agent of this embodiment is silane coupling agent KH560.

[0048] Example 3. The highly efficient ammonia nitrogen degrading bacteria of this embodiment include the following raw materials in parts by weight: 10-15 parts of ammonia oxidizing bacteria, 10-15 parts of full-process nitrifying bacteria, 5-8 parts of calcium-acetic acid Acinetobacter, 2-5 parts of Bacillus licheniformis, 2-5 parts of denitrifying bacteria, 2-5 parts of complex enzymes and 5-8 parts of nutrients.

[0049] The complex enzyme of this embodiment is a mixture of protease, lipase and amylase in a weight ratio of 1:1:1; the nutrient is prepared by 3.5 parts of peptone, 2 parts of beef extract and 3.5 parts of sodium chloride.

[0050] The high-efficiency ammonia nitrogen-degrading bacteria of this embodiment also include 3.5 parts of additives.

[0051] The preparation method of the additive of this embodiment is: S01: heat-treating the nano-titanium dioxide at 57°C for 7.5 minutes, and then immersing it in a sufficient amount of a conditioning agent for ultrasonic modification. After the ultrasonic treatment is completed, an additive solution is obtained; S02: Stir and mix the carbon nanotubes in a sufficient amount of hydrogen peroxide solution, then wash and dry them, stir and modify the dried carbon nanotubes and the additive solution in a weight ratio of 2:5, and after stirring, wash and dry them to obtain the additive.

[0052] The ultrasonic power of the ultrasonic modification treatment in this embodiment is 470W, and the ultrasonic time is 25min.

[0053] The mass fraction of the hydrogen peroxide solution in this embodiment is 3.5%.

[0054] The stirring speed of the stirring modification treatment in this embodiment is 500 r / min, and the stirring time is 25 min.

[0055] The preparation method of the regulator of this embodiment is: 3.5 parts of dodecylbenzenesulfonic acid solution, 2 parts of silane coupling agent and 3 parts of nano-wollastonite were blended and added into 5.5 parts of dopamine hydrochloride solution, and the blending was continued to obtain a regulator.

[0056] The mass fraction of the dopamine hydrochloride solution in this embodiment is 3.5%; the mass fraction of the dodecylbenzenesulfonic acid solution is 4.5%.

[0057] The silane coupling agent of this embodiment is silane coupling agent KH560.

[0058] Comparative Example 1. The difference from Example 3 is that no additives were added.

[0059] Comparative Example 2. The difference from Example 3 is that the preparation method of the additive is different; The carbon nanotubes are first stirred and mixed in a sufficient amount of hydrogen peroxide solution, then washed with water and dried, and the dried carbon nanotubes and the regulator are stirred and modified in a weight ratio of 2:5. After the stirring is completed, the additive is washed with water and dried to obtain the additive. The preparation method of the regulator is: 3.5 parts of dodecylbenzenesulfonic acid solution, 2 parts of silane coupling agent and 3 parts of nano-wollastonite were blended and added into 5.5 parts of dopamine hydrochloride solution, and the blending was continued to obtain a regulator.

[0060] Comparative Example 3. The difference from Example 3 is that no dried carbon nanotubes are added in the preparation of the additive.

[0061] Comparative Example 4. The difference from Example 3 is that the preparation method of the regulator is different; 3.5 parts of dodecylbenzenesulfonic acid solution were added to 5.5 parts of dopamine hydrochloride solution and mixed thoroughly to obtain a regulator.

[0062] The product performance tests of Examples 1-3 and Comparative Examples 1-4 are as follows:

[0063] It can be seen from Comparative Examples 1-4 and Example 3 that the performance of the product deteriorated significantly when no additives were added. At the same time, the performance of the product tended to deteriorate when the preparation methods of the additives were different, when the dried carbon nanotubes were not added, and when the preparation methods of the regulators were different. Only when the additives were obtained by the method of the present invention, the product performance effect was the most significant. High-efficiency ammonia nitrogen degrading bacteria are made of ammonia oxidizing bacteria, full-process nitrifying bacteria and other raw materials. By adding complex enzymes and nutrients as functional additives, and adding additives to coordinate the degradation bacteria, the anaerobic bacteria can make full use of the N source, C source, P source and other sources in the wastewater for life activities, and convert NH3-N into N02-, N02-. At the same time, it consumes a large amount of organic matter and converts the organic matter into CO2, H2O, 5-8 parts, so that the ammonia nitrogen and COD in the water body can be reduced at the same time. This bacterial agent is enriched with multiple populations of anaerobic and facultative anaerobic nitrifying bacteria and denitrifying bacteria, which can mainly degrade ammonia nitrogen, eliminate sediment, strengthen the indigenous microbial flora of sediment, establish microbial ecological flora, and is used for the treatment of high ammonia nitrogen water bodies and the elimination of black and odorous sediment.

[0064] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be considered exemplary and non-restrictive in all respects, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention.

[0065] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A highly efficient ammonia nitrogen degrading bacterium, characterized in that: It comprises the following raw materials in parts by weight; 10-15 parts of ammonia oxidizing bacteria, 10-15 parts of full-process nitrifying bacteria, 5-8 parts of calcium-acetic acid Acinetobacter, 2-5 parts of Bacillus licheniformis, 2-5 parts of denitrifying bacteria, 2-5 parts of complex enzymes and 5-8 parts of nutrients.

2. The highly efficient ammonia-nitrogen degrading bacteria according to claim 1, characterized in that: The complex enzyme is a mixture of protease, lipase and amylase in a weight ratio of 1:1:1; the nutrient is prepared by 2-5 parts of peptone, 1-3 parts of beef extract and 2-5 parts of sodium chloride.

3. The highly efficient ammonia-nitrogen degrading bacteria according to claim 1, characterized in that: The high-efficiency ammonia nitrogen-degrading bacteria also include 2-5 parts of additives.

4. The highly efficient ammonia-nitrogen degrading bacteria according to claim 3, characterized in that: The preparation method of the additive is: S01: heat-treating the nano-titanium dioxide at 55-60°C for 5-10 minutes, and then immersing it in a sufficient amount of a conditioning agent for ultrasonic modification. After the ultrasonic modification is completed, an additive solution is obtained; S02: Stir and mix the carbon nanotubes in a sufficient amount of hydrogen peroxide solution, then wash and dry them, stir and modify the dried carbon nanotubes and the additive solution in a weight ratio of 2:5, and after stirring, wash and dry them to obtain the additive.

5. The highly efficient ammonia-nitrogen degrading bacteria according to claim 4, characterized in that: The ultrasonic power of the ultrasonic modification treatment is 450-500W, and the ultrasonic time is 20-30min.

6. The highly efficient ammonia-nitrogen degrading bacteria according to claim 4, characterized in that: The mass fraction of the hydrogen peroxide solution is 2-5%.

7. The highly efficient ammonia-nitrogen degrading bacteria according to claim 4, characterized in that: The stirring speed of the stirring modification treatment is 450-550 r / min, and the stirring time is 20-30 min.

8. The highly efficient ammonia-nitrogen degrading bacteria according to claim 4, characterized in that: The preparation method of the regulator is: 2-5 parts of dodecylbenzenesulfonic acid solution, 1-3 parts of silane coupling agent and 2-4 parts of nano-wollastonite are mixed and added into 4-7 parts of dopamine hydrochloride solution, and the mixture is continued to be fully mixed to obtain a regulator.

9. The highly efficient ammonia-nitrogen degrading bacteria according to claim 8, characterized in that: The mass fraction of the dopamine hydrochloride solution is 2-5%; the mass fraction of the dodecylbenzenesulfonic acid solution is 3-6%; and the silane coupling agent is silane coupling agent KH560.

10. Use of the efficient ammonia nitrogen degrading bacteria according to any one of claims 1 to 9 in aquaculture.

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