A protein detection kit

Abstract

The present invention relates to the technical field of protein kits, and specifically discloses a protein detection kit, which includes a lower box body. An upper box body is movably installed above the lower box body, and a second movable plate is movably installed on one side of the bottom end of the inner wall of the lower box body. By providing the second movable plate, the first movable plate and the adapter, through the lifting of the second movable plate, during the process of culturing and adding reagents to the microplate, it is not necessary for the staff to manually pick up the microplate, which increases the convenience of using the device. At the same time, it avoids the risk of dropping when the staff manually picks up the microplate. Through the first movable plate and the adapter, after the incubation of the microplate is completed, the washing work can be directly carried out inside the device, so that the microplate does not need to withstand temperature changes, avoiding too large a temperature change difference borne by the microplate, which affects the biological activity of the samples detected inside the microplate and increases the accuracy of the data finally measured by the device.

Description

Technical Field

[0001] The present invention relates to the technical field of protein kits, and particularly to a protein detection kit. Background Art

[0002] Prostate diseases are a major category of diseases affecting men in our country. Especially now, the incidence of prostatitis is increasing. Therefore, the prevention and detection of prostate diseases are extremely important. Currently, the prevention and detection methods for prostate diseases mostly involve the detection of prostatic small body exfoliated proteins, and the detection of prostatic small body proteins requires the use of a prostatic small body exfoliated protein detection kit.

[0003] Currently, the kit only has the function of storing reagents, and its function is too single. Moreover, during the detection of prostatic small body exfoliated proteins, multiple operating tools are required for the microplate in multiple operating steps. After adding samples to the microplate for incubation, the microplate needs to be washed. This operating step often requires taking out the microplate, and then the staff uses a machine or manually washes the microplate. Subsequently, a chromogenic agent is added to the microplate, and the samples detected in the microplate are caused to change color through re-incubation. Finally, the reaction is terminated by adding a termination solution, and the color of the samples detected in the microplate is observed using an instrument to complete the detection of prostatic small body exfoliated proteins. During this detection process, an incubator is often used for microplate incubation. Therefore, the method of taking out the microplate for washing will cause too large a temperature change difference borne by the microplate, affecting the biological activity of the samples detected inside the microplate and the accuracy of the data obtained from the final experiment. Summary of the Invention

[0004] The purpose of the present invention is to solve the deficiencies existing in the prior art, and a protein detection kit is proposed.

[0005] In order to achieve the above purpose, the present invention adopts the following technical solutions:

[0006] A protein detection kit includes a lower box body. An upper box body is movably installed above the lower box body. One side of the bottom end of the inner wall of the lower box body is movably installed with a second movable plate. Multiple microplates are placed on the top of the second movable plate. An electrothermal constant temperature plate is embedded inside the second movable plate. A rotating rod is movably installed at the bottom end of the inner wall of the upper box body. A washing mechanism is provided on the rotating rod. Third movable plates are movably installed on both side walls of the lower box body. A plurality of stable grooves are evenly opened at the bottom end of the inner wall of the lower box body on the side far from the second movable plate. Reagent bottles are placed inside the stable grooves. Two first openings are penetrated through the top of the upper box body.

[0007] Preferably, a first slot is penetrated and opened on the inner wall of the upper box body above the second movable plate, and a rotating plate is movably installed inside the first slot.

[0008] Preferably, second slots are opened on the inner wall at both ends of one side of the first slot, a rotating shaft is inserted and installed inside the second slots, and one end of the rotating shaft close to the rotating plate is connected to the rotating plate.

[0009] Preferably, first electric telescopic rods are embedded and installed at the four corners of the lower box body, the telescopic ends of the first electric telescopic rods face upward, and the telescopic ends of the first electric telescopic rods are connected to the bottom of the upper box body.

[0010] Preferably, a plugging slot is opened on one side of the bottom end of the inner wall of the lower box body, a third electric telescopic rod is inserted and installed inside the plugging slot, the telescopic end of the third electric telescopic rod faces upward, and the telescopic end of the third electric telescopic rod is embedded and installed into the bottom of the second movable plate.

[0011] Preferably, a rotating motor is embedded and installed at the bottom end of the inner wall of the upper box body, the output end of the rotating motor faces downward, and the output end of the rotating motor is connected to the top of the rotating rod.

[0012] Preferably, the washing mechanism includes a first movable plate, second openings and a swivel joint. Two first movable plates are movably installed at the bottom of the rotating rod, a plurality of swivel joints are evenly installed at the bottom of the first movable plate, a plurality of second openings are evenly opened on both sides of the two first movable plates away from each other, the number and position of the second openings correspond to those of the swivel joints, and the second openings are communicated with the swivel joints.

[0013] Preferably, a first sliding groove is horizontally opened along the length direction at the bottom of the rotating rod, a first electric slider is installed at the top of the first movable plate, the first electric slider is slidably installed inside the first sliding groove, and an electric extension rod is embedded and installed at the top of the first movable plate, and the installation end of the electric extension rod is connected to the first electric slider.

[0014] Preferably, second sliding grooves are horizontally opened on both side walls of the lower box body, second electric sliders are installed on the side of the third movable plate close to the second sliding grooves, and the second electric sliders are slidably installed inside the second sliding grooves.

[0015] Preferably, a second electric telescopic rod is embedded and installed on the side of the third movable plate close to the second electric slider, and the installation end of the second electric telescopic rod is connected to the second electric slider.

[0016] Compared with the prior art, the beneficial effects of the present invention are:

[0017] In the present invention, a second movable plate, a first movable plate and a adapter are provided. By lifting the second movable plate, during the process of cultivating and adding reagents to the microplate, it is not necessary for the staff to manually pick up the microplate, which increases the convenience of using the device. At the same time, the risk of dropping when the staff manually picks up the microplate is avoided. Through the first movable plate and the adapter, after the incubation of the microplate is completed, the washing work can be directly carried out inside the device, so that the microplate does not need to bear temperature changes, and the difference in temperature changes borne by the microplate is avoided from being too large, which affects the biological activity of the samples detected inside the microplate, and increases the accuracy of the data finally measured by the device;

[0018] The electric extension rod drives the first movable plate to move downward. When the bottom of the first movable plate moves below the middle section of the reagent bottle, the two first movable plates move closer to each other until the two first movable plates clamp the reagent bottle. Then, the first electric telescopic rod drives the upper box body to move upward. When the upper box body rises to the preset height position, the first electric telescopic rod stops extending. At this time, the first movable plate clamps the reagent bottle upward, which is convenient for the staff to pick up the reagent bottle. The staff extends the medical tray to the bottom of the reagent bottle, and then the two first movable plates move away from each other, and the reagent bottle can be dropped onto the medical tray. In this way, the time for the staff to pick up the reagent bottle can be reduced, and the convenience of using the device can be increased;

[0019] When the device is stored or transferred, multiple microplates are stacked on the top of the second movable plate. At this time, the third electric telescopic rod drives the second movable plate to extend upward until the top of the microplate on the top of the second movable plate abuts against the bottom of the rotating plate. At this time, through the abutment of the second movable plate and the rotating plate on the microplate, the microplate is stored more stably inside the device, so that the microplate will not move during the storage or transfer of the reagent bottle, and the situation that the microplate is damaged by collision inside the device is avoided, which increases the convenience of using the device.

[0020] In the present invention, a third movable plate is provided. When the device is stored or transferred, the reagent bottle is placed inside the stable groove. The two third movable plates move to the position of the reagent bottle, and the two third movable plates move closer to each other until the two third movable plates abut against the outer side of the reagent bottle. At this time, by abutting the third movable plate against the reagent bottle, the reagent bottle can be fixed, so that the reagent bottle is more stable during the storage or transfer process, and the situation that the reagent bottle is damaged by collision inside the device is avoided, which increases the storage safety of the reagent bottle;

[0021] During the incubation process in the microplate, the two third movable plates move to the second movable plate through the sliding of the second electric slider. Through the extension of the second electric telescopic rod, the two second movable plates move closer to each other and abut against the microplate. At this time, through the abutment of the second movable plate against the microplate, the storage stability of the microplate during the experiment inside the device can be increased, preventing the device itself from colliding and causing the displacement and overturning of the microplate, resulting in the failure of detection.

[0022] When the second movable plate clamps the microplate, on the premise that the two second movable plates clamp the microplate, through the relative telescopic movement of the two second electric telescopic rods, at this time, the second movable plate can shake the microplate, increasing the contact between the sample and the microplate, discharging the bubbles in the sample, and enhancing the incubation effect of the sample inside the microplate.

[0023] When the microplate needs to be washed, the third movable plate clamps the microplate and moves it to the lower part of the first movable plate. By driving the microplate to move under the first movable plate through the third movable plate, the microplate can be quickly washed and liquid can be pumped out, increasing the convenience of using the device. Description of the Drawings

[0024] Figure 1 It is a schematic diagram of the overall sectional structure of the present invention;

[0025] Figure 2 It is a schematic diagram of the overall structure of the present invention;

[0026] Figure 3 It is a schematic diagram of the structure of the upper box body of the present invention;

[0027] Figure 4 It is a schematic diagram of the installation structure of the rotating plate and the rotating shaft of the present invention;

[0028] Figure 5 It is a schematic diagram of the installation structure of the first movable plate and the rotating rod of the present invention;

[0029] Figure 6 It is a schematic diagram of the installation structure of the first electric slider of the present invention;

[0030] Figure 7 It is a schematic diagram of the installation structure of the rotating motor of the present invention;

[0031] Figure 8 It is a schematic diagram of the installation structure of the electric extension rod of the present invention;

[0032] Figure 9 It is a schematic diagram of the installation structure of the second movable plate and the third movable plate of the present invention;

[0033] Figure 10 It is a schematic diagram of the structure of the lower box body of the present invention;

[0034] Figure 11 Schematic diagram of the installation structure of the second electric telescopic rod of the present invention;

[0035] Figure 12 Schematic diagram of the installation structure of the third electric telescopic rod of the present invention.

[0036] In the figure: 1. Lower box body; 2. Upper box body; 3. First slot; 4. Rotating plate; 5. First opening; 6. First movable plate; 7. Second movable plate; 8. Microporous plate; 9. Third movable plate; 10. Reagent bottle; 11. Second slot; 12. Rotating shaft; 13. Rotating rod; 14. Second opening; 15. Adapter; 16. First chute; 17. First electric slider; 18. Rotating motor; 19. First electric telescopic rod; 20. Stable groove; 21. Insertion groove; 22. Second chute; 23. Second electric slider; 24. Second electric telescopic rod; 25. Third electric telescopic rod; 26. Electric extension rod. Specific implementation manners

[0037] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments.

[0038] Referring to Figures 1-12 , a protein detection kit includes a lower box body 1, an upper box body 2 is movably installed above the lower box body 1, a second movable plate 7 is movably installed on one side of the inner wall bottom end of the lower box body 1, a plurality of microporous plates 8 are placed on the top of the second movable plate 7, a thermostatic heating plate is embedded in the second movable plate 7, a rotating rod 13 is movably installed on the inner wall bottom end of the upper box body 2, and a washing mechanism is provided on the rotating rod 13. Third movable plates 9 are movably installed on both side walls of the lower box body 1. A plurality of stable grooves 20 are evenly formed in the inner wall bottom end of the lower box body 1 on the side far from the second movable plate 7, and reagent bottles 10 are placed in the stable grooves 20. Two first openings 5 penetrate through the top of the upper box body 2. The thermostatic incubation of the samples on the microporous plate 8 can be realized through the thermostatic heating plate on the second movable plate 7. The microporous plate 8 is incubated at a constant temperature inside the device, and then washed inside the device through the washing mechanism, so that the microporous plate 8 does not need to be taken out multiple times, resulting in too large a difference in the temperature borne by the microporous plate 8, and reducing the influence of the experimental process on the final experimental measurement data.

[0039] As a technical optimization solution of the present invention, a first slot 3 penetrates through the inner wall of the upper box body 2 above the second movable plate 7, and a rotating plate 4 is movably installed inside the first slot 3. Through the rotating plate 4 installed at the first slot 3, the rotating plate 4 can block the first slot 3, and the normal operation of the second movable plate 7 will not be affected after the rotating plate 4 is opened.

[0040] As a technical optimization solution of the present invention, second slots 11 are provided on the inner walls at both ends of one side of the first slot 3. A rotating shaft 12 is inserted and installed inside the second slot 11. One end of the rotating shaft 12 close to the rotating plate 4 is connected to the rotating plate 4. The rotating shaft 12 drives the rotating plate 4 to rotate upward until the rotating plate 4 rotates to a vertically upward state, so that the second movable plate 7 can operate normally.

[0041] As a technical optimization solution of the present invention, first electric telescopic rods 19 are embedded at the four corners of the lower box body 1. The telescopic ends of the first electric telescopic rods 19 face upward, and the telescopic ends of the first electric telescopic rods 19 are connected to the bottom of the upper box body 2. By extending the first electric telescopic rods 19, the upper box body 2 can be extended upward, facilitating the corresponding operations of the staff on the mechanisms inside the device, and also facilitating the subsequent maintenance of the inside of the device by the staff.

[0042] As a technical optimization solution of the present invention, a plug-in slot 21 is provided on one side of the bottom end of the inner wall of the lower box body 1. A third electric telescopic rod 25 is inserted and installed inside the plug-in slot 21. The telescopic end of the third electric telescopic rod 25 faces upward, and the telescopic end of the third electric telescopic rod 25 is embedded and installed into the bottom of the second movable plate 7. The third electric telescopic rod 25 can drive the second movable plate 7 to perform corresponding extension.

[0043] As a technical optimization solution of the present invention, a rotating motor 18 is embedded at the bottom end of the inner wall of the upper box body 2. The output end of the rotating motor 18 faces downward, and the output end of the rotating motor 18 is connected to the top of the rotating rod 13. The rotating motor 18 can drive the rotating rod 13 to rotate, so that the rotating rod 13 can be adjusted to the corresponding use state according to different use requirements.

[0044] As a technical optimization solution of the present invention, the washing mechanism includes a first movable plate 6, second openings 14 and adapters 15. Two first movable plates 6 are movably installed at the bottom of the rotating rod 13. A plurality of adapters 15 are evenly installed at the bottom of the first movable plate 6. A plurality of second openings 14 are evenly provided on the mutually remote sides of the two first movable plates 6. The number and positions of the second openings 14 and the adapters 15 correspond, and the second openings 14 are communicated with the adapters 15. By using the two adapters 15, the washing work of the microplate 8 can be completed without transferring the microplate 8, so that the microplate 8 does not need to bear temperature changes, increasing the accuracy of the data finally measured by the device.

[0045] As a technical optimization solution of the present invention, a first chute 16 is horizontally opened along the length direction at the bottom of the rotating rod 13. A first electric slider 17 is installed on the top of the first movable plate 6. The first electric slider 17 is slidably installed inside the first chute 16. An electric extension rod 26 is embedded and installed on the top of the first movable plate 6. The installation end of the electric extension rod 26 is connected to the first electric slider 17. By the sliding of the first electric slider 17 in the first chute 16, the first movable plate 6 can be driven to make corresponding position adjustments. By the telescoping of the electric extension rod 26, the first movable plate 6 can be switched to corresponding usage states according to different usage requirements, increasing the stability of the usage function of the device.

[0046] As a technical optimization solution of the present invention, second chutes 22 are horizontally opened on both side walls of the lower box body 1. A second electric slider 23 is installed on one side of the third movable plate 9 close to the second chute 22. The second electric slider 23 is slidably installed inside the second chute 22. By the sliding of the second electric slider 23 in the second chute 22, the third movable plate 9 can be driven to make corresponding movements inside the lower box body 1.

[0047] As a technical optimization solution of the present invention, a second electric telescopic rod 24 is embedded and installed on one side of the third movable plate 9 close to the second electric slider 23. The installation end of the second electric telescopic rod 24 is connected to the second electric slider 23. By driving the third movable plate 9 to telescope by the second electric telescopic rod 24, the third movable plate 9 can achieve a variety of usage effects, increasing the convenience of use of the device.

[0048] When the present invention is in use, a background control system is provided on the device to control the electrical mechanisms in the device. The device drives the electrical mechanisms in the device by connecting to an external power source through a wire. A flow control valve is installed on the adapter 15, and each adapter 15 is connected to a conduit branch pipe. The conduit branch pipe passes through the second opening 14 corresponding to the adapter 15, and then the ends of all the conduit branch pipes on one first movable plate 6 away from the first movable plate 6 are all connected to a conduit main pipe. The end of the conduit main pipe away from the conduit branch pipe passes through a first opening 5 and is located outside the upper box body 2. When the device is in use, the conduit main pipe on one first movable plate 6 is connected to a preset liquid suction pump outside the device, and a disposable suction head is sleeved and installed at the end of the adapter 15 on this first movable plate 6 away from the first movable plate 6. The disposable suction head is a mature existing product, so no more elaboration will be made on it here. The conduit main pipe on the other first movable plate 6 is connected to a preset liquid adding pump outside the device, and the liquid adding pump adds a special cleaning liquid to the device.

[0049] The lengths of the conduit branch pipes installed on the two first movable plates 6 are sufficient for the first movable plates 6 to complete different displacement processes inside the device. When the device is not in use, the main conduit pipes on the two first movable plates 6 are disconnected from the external connection device, and then the staff stores the device in a suitable temperature environment according to the storage requirements. A camera is installed at the top of the inner wall of the upper box body 2, and a temperature sensor is installed inside the lower box body 1. The camera and the temperature sensor are both existing mature technologies, so no more elaboration will be made on them. The rotating shaft 12 is electrically driven.

[0050] When the device is being stored or transferred, a reagent bottle 10 is placed inside the stabilizing groove 20, and multiple microplates 8 are stacked on the top of the second movable plate 7. At this time, by sliding the second electric slider 23 in the second chute 22, the two third movable plates 9 are moved to the position of the reagent bottle 10. Then, the second electric telescopic rod 24 drives the third movable plate 9 to extend, so that the two third movable plates 9 move towards each other until the two third movable plates 9 abut against the outer side of the reagent bottle 10. At this time, by the third movable plate 9 abutting against the reagent bottle 10, the reagent bottle 10 can be fixed, making the reagent bottle 10 more stable during storage or transfer, avoiding the situation where the reagent bottle 10 collides and is damaged inside the device, and increasing the storage safety of the reagent bottle 10. Multiple microplates 8 are stacked on the top of the second movable plate 7. At this time, the third electric telescopic rod 25 drives the second movable plate 7 to extend upward until the top of the microplate 8 on the top of the second movable plate 7 abuts against the bottom of the rotating plate 4. At this time, by the second movable plate 7 and the rotating plate 4 abutting against the microplate 8, the microplate 8 is stored more stably inside the device, so that the microplate 8 will not move during the storage or transfer of the reagent bottle 10, avoiding the situation where the microplate 8 collides and is damaged inside the device, and increasing the use convenience of the device.

[0051] When it is necessary to detect a sample, the staff takes out the device and uses it according to the usage requirements. After connecting the conduits on the device to the liquid suction pump and the liquid addition pump respectively, the device is powered on. Then, the rotating plate 4 is driven to rotate upward by the rotating shaft 12 until the rotating plate 4 rotates to a vertically upward state. At this time, the second movable plate 7 is driven to extend upward by the third electric telescopic rod 25 until the top of the second movable plate 7 extends to the same horizontal height as the top of the upper box body 2. At this time, the stacked microplates 8 are removed by the staff, and one microplate 8 is left for experimental use. The two third movable plates 9 move away from each other until the third movable plate 9 moves to a state where it abuts against the side wall of the lower box body 1. At this time, the first movable plate 6 is driven to move downward by the electric extension rod 26. When the bottom of the first movable plate 6 moves below the middle section of the reagent bottle 10, the two first movable plates 6 are driven to move closer to each other by the sliding of the first electric slider 17 in the first chute 16 until the two first movable plates 6 clamp the reagent bottle 10;

[0052] Then, the upper box body 2 is driven to move upward by the first electric telescopic rod 19. When the upper box body 2 rises to the preset height position, the first electric telescopic rod 19 stops extending. At this time, the first movable plate 6 clamps the reagent bottle 10 upward, which is convenient for the staff to take the reagent bottle 10. The staff extends the medical tray to the bottom of the reagent bottle 10. Then, the two first movable plates 6 move away from each other, and the reagent bottle 10 can be dropped onto the medical tray. In this way, the time for the staff to take the reagent bottle 10 can be reduced, and the usability of the device can be increased. The staff plugs and installs the pre-prepared disposable pipette tip on the adapter 15 connected to the liquid suction pump. Then, the staff prepares the reagent as required. At this time, the microplate 8 left for the experiment is placed on the top of the second movable plate 7. The staff adds the sample to be detected into the microplate 8. Then, the second movable plate 7 is driven to contract downward by the third electric telescopic rod 25 until the bottom of the second movable plate 7 abuts against the inner bottom wall of the lower box body 1.

[0053] Subsequently, two third movable plates 9 are slid to the second movable plate 7 by the second electric slider 23. The second electric telescopic rod 24 extends to make the two second movable plates 7 move closer to each other and abut against the microplate 8. At this time, by abutting the second movable plate 7 against the microplate 8, the storage stability of the microplate 8 during the experiment inside the device can be increased, preventing the device itself from colliding and causing the displacement and overturning of the microplate 8, resulting in the failure of detection. When the second movable plate 7 clamps the microplate 8, on the premise that the two second movable plates 7 clamp the microplate 8, the two second electric telescopic rods 24 perform relative telescopic movements. At this time, the second movable plate 7 can shake the microplate 8 to increase the contact between the sample and the microplate 8, discharge the bubbles in the sample, and improve the incubation effect of the sample inside the microplate 8.

[0054] The electrothermal constant temperature plate installed inside the second movable plate 7 is activated to control the temperature of the second movable plate 7 and the inside of the device. Through the monitoring of the temperature inside the device by the temperature sensor, the internal control of the device is set at the appropriate incubation temperature for the sample. After incubation for a preset time, the incubation of the sample inside the microplate 8 is completed. Subsequently, with the two third movable plates 9 clamping the microplate 8, they move away from the second movable plate 7 until the third movable plate 9 clamps the microplate 8 and moves it below the first movable plate 6.

[0055] The rotating motor 18 drives the rotating rod 13 to rotate until the length direction of the first movable plate 6 rotates to be horizontal with the length direction of the upper box body 2. Subsequently, the third movable plate 9 drives the microplate 8 to move below the first movable plate 6. When the sample incubation hole on the microplate 8 moves below the adapter 15 for installing the disposable pipette tip, at this time, the electric extension rod 26 drives the first movable plate 6 to move downward, so that the disposable pipette tip on the first movable plate 6 extends into the sample to suck away the unreacted sample liquid. Subsequently, the third movable plate 9 drives the microplate 8 to move below another first movable plate 6. Then, the first movable plate 6 here extends downward to the sample incubation hole on the microplate 8, and the adapter 15 here injects the washing liquid into the sample incubation hole. Subsequently, the washing liquid is sucked out by the pipette tip. According to the operation requirements, the washing operation is repeated multiple times to complete the washing work of the sample on the microplate 8.

[0056] After the washing is completed, the third movable plate 9 moves the microplate 8 to the top of the second movable plate 7 for storage. Subsequently, the third movable plate 9 stops clamping the microplate 8, the rotating plate 4 opens upward, and the second movable plate 7 drives the microplate 8 to move upward. Then, the staff adds the previously prepared chromogenic agent into the sample. The second movable plate 7 moves the microplate 8 into the interior of the device again. Subsequently, after incubation for a preset time, the second movable plate 7 moves the microplate 8 out of the interior of the device again. At this time, the staff adds the termination solution into the sample to terminate the reaction of the sample. Then, the staff takes the microplate 8 to the microplate reader to test the PSEP concentration value, and the detection of the prostate small extracellular protein this time can be completed.

[0057] After the device is used up, the upper box body 2 can be lifted to a preset height position by the first electric telescopic rod 19. Subsequently, the rotating motor 18 drives the rotating rod 13 to rotate until the length direction of the rotating rod 13 is in the same horizontal direction as the end direction of the upper box body 2. At this time, the first movable plate 6 equipped with the disposable pipette tip moves in the direction close to the end wall of the upper box body 2. When it moves to the preset position, the staff removes the disposable pipette tip installed on the adapter 15, and installs a new disposable pipette tip when the device is used next time.

[0058] The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution and inventive concept of the present invention, makes equivalent substitutions or changes, and should be covered by the protection scope of the present invention.

Claims

1. A protein detection kit, comprising a lower box body (1), characterized in that: An upper box body (2) is movably mounted above the lower box body (1); a second movable plate (7) is movably mounted on one side of the bottom end of the inner wall of the lower box body (1); a plurality of microplates (8) are placed on the top of the second movable plate (7); an electric heating constant temperature plate is embedded and mounted inside the second movable plate (7); a rotating rod (13) is movably mounted on the bottom end of the inner wall of the upper box body (2); a washing mechanism is arranged on the rotating rod (13); third movable plates (9) are movably mounted on both side walls of the lower box body (1); a plurality of stabilizing grooves (20) are evenly arranged on the bottom end of the inner wall of the lower box body (1) away from the second movable plate (7); a reagent bottle (10) is placed inside the stabilizing groove (20); and two first openings (5) are arranged through the top of the upper box body (2); The washing mechanism comprises a first movable plate (6), a second opening (14) and an adapter (15); two first movable plates (6) are movably mounted at the bottom of the rotating rod (13); a plurality of adapters (15) are evenly mounted at the bottom of the first movable plates (6); a plurality of second openings (14) are evenly opened on one side of the two first movable plates (6) away from each other; the number and position of the second openings (14) and the adapter (15) correspond to each other, and the second openings (14) and the adapter (15) are connected; A first slide groove (16) is horizontally provided at the bottom of the rotating rod (13) along the length direction; a first electric slider (17) is installed at the top of the first movable plate (6); the first electric slider (17) is slidably installed inside the first slide groove (16); an electric extension rod (26) is embedded and installed at the top of the first movable plate (6); and the installation end of the electric extension rod (26) is connected to the first electric slider (17).

2. A protein detection kit according to claim 1, characterized in that: The upper box body (2) has a first slot (3) extending through the inner wall above the second movable plate (7), and a rotating plate (4) is movably mounted inside the first slot (3).

3. A protein detection kit according to claim 2, characterized in that: Second slots (11) are provided on the inner walls at both ends of one side of the first slot (3), a rotating shaft (12) is inserted and installed inside the second slot (11), and one end of the rotating shaft (12) close to the rotating plate (4) is connected to the rotating plate (4).

4. A protein detection kit according to claim 1, characterized in that: A first electric telescopic rod (19) is embedded and installed at each of the four corners of the lower box body (1), the telescopic end of the first electric telescopic rod (19) faces upward, and the telescopic end of the first electric telescopic rod (19) is connected to the bottom of the upper box body (2).

5. A protein detection kit according to claim 1, characterized in that: A plug-in slot (21) is provided on one side of the bottom end of the inner wall of the lower box body (1), and a third electric telescopic rod (25) is plugged and installed inside the plug-in slot (21), with the telescopic end of the third electric telescopic rod (25) facing upward, and the telescopic end of the third electric telescopic rod (25) is embedded and installed in the bottom of the second movable plate (7).

6. A protein detection kit according to claim 1, characterized in that: A rotating motor (18) is embedded and installed at the bottom end of the inner wall of the upper box body (2), the output end of the rotating motor (18) is downward, and the output end of the rotating motor (18) is connected to the top of the rotating rod (13).

7. A protein detection kit according to claim 1, characterized in that: Second slide grooves (22) are horizontally provided on both side walls of the lower box body (1), and a second electric slider (23) is installed on one side of the third movable plate (9) close to the second slide groove (22). The second electric slider (23) is slidably installed inside the second slide groove (22).

8. A protein detection kit according to claim 7, characterized in that: A second electric telescopic rod (24) is embedded and installed on one side of the third movable plate (9) close to the second electric slide block (23); the installation end of the second electric telescopic rod (24) is connected to the second electric slide block (23).

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