Application of biological antibacterial agent to antagonism of plant pathogenic fungi, prevention and treatment of plant diseases and preparation of plant disease prevention and treatment agent, and plant disease prevention and treatment agent

Abstract

The invention relates to application of a biological antibacterial agent to antagonism of plant pathogenic fungi, prevention and treatment of plant diseases and preparation of a plant disease prevention and treatment agent and the plant disease prevention and treatment agent, and belongs to the technical field of microorganisms. Volatile metabolites of Irpex lacteus have an obvious inhibiting effect on growth of botrytis cinerea, alternaria alternata and fusarium oxysporum, and can be used for antagonizing plant pathogenic fungi (botrytis cinerea, alternaria alternata and fusarium oxysporum), so that the situation that pesticide residues are easily caused when pesticides are used, and the human and livestock health is seriously harmed is avoided.

Description

Technical Field

[0001] The invention relates to application of a biological antibacterial agent in antagonizing plant pathogenic fungi, preventing and controlling plant diseases, and preparing a plant disease preventing and controlling agent, and belongs to the technical field of microorganisms. Background Art

[0002] At present, chemical control is still the main method for controlling plant pathogenic fungi, but the long-term and unscientific use of large amounts of chemical pesticides can easily lead to the development of pathogen resistance, resulting in reduced control effects. In order to improve the effect, people often continue to increase the amount of pesticides, which eventually leads to pesticide residues in crops, seriously endangering the health of humans and animals.

[0003] In the past few decades, volatile metabolomics has flourished. In the early days, people mainly explored the complex mechanisms of interactions between plants, plants and insects, and insects. In recent years, since volatile metabolites produced by bacteria or fungi can also produce induction effects between plants or insects, people have also begun to study the inhibitory effects of volatile metabolites of biocontrol fungi on pathogens or pests, as well as the induced resistance effects of volatile metabolites of biocontrol fungi on plants. White rot fungus is a white rot fungus distributed worldwide. However, there are no reports on the use of volatile metabolites of white rot fungi to inhibit plant pathogenic fungi. Summary of the invention

[0004] The purpose of the present invention is to provide an application of a biological antibacterial agent in antagonizing plant pathogenic fungi, which can solve the problem that the current use of pesticides to antagonize plant pathogenic fungi easily causes pesticide residues and seriously endangers the health of humans and animals.

[0005] The second purpose of the present invention is to provide an application of a biological antibacterial agent in preventing and controlling plant diseases, which can solve the problem that the current use of pesticides to prevent and control plant diseases easily causes pesticide residues and seriously endangers the health of humans and animals.

[0006] The third object of the present invention is to provide an application of a biological antibacterial agent in the preparation of a plant disease control agent, which can solve the problem that the current use of pesticides to control plant diseases easily causes pesticide residues and seriously endangers the health of humans and animals.

[0007] The fourth object of the present invention is to provide a plant disease control agent that can solve the problem that the current use of pesticides to control plant diseases easily results in pesticide residues that seriously endanger the health of humans and animals.

[0008] In order to achieve the above objectives, the technical solution adopted by the application of the biological antibacterial agent of the present invention in antagonizing plant pathogenic fungi is:

[0009] The invention discloses an application of a biological antimicrobial agent in antagonizing plant pathogenic fungi, wherein the biological antimicrobial agent is white sac fungus, and the plant pathogenic fungi are pepper brown mold pathogen and / or cucumber wilt pathogen; or the biological antimicrobial agent is a volatile metabolite of white sac fungus, and the plant pathogenic fungi are one, two or three of tomato gray mold pathogen, pepper brown mold pathogen and cucumber wilt pathogen.

[0010] The experimental results show that white capsule rake tooth fungus and / or its volatile metabolites have a significant inhibitory effect on the growth of tomato gray mold, pepper red star pathogen, and cucumber wilt pathogen, and can be used to antagonize plant pathogenic fungi (tomato gray mold, pepper red star pathogen, cucumber wilt pathogen), thereby avoiding the use of pesticides that easily cause pesticide residues and seriously endanger the health of humans and animals.

[0011] The technical solution adopted by the application of the biological antibacterial agent of the present invention in preventing and controlling plant diseases is:

[0012] The application of a biological antimicrobial agent in preventing and controlling plant diseases, wherein the biological antimicrobial agent is white sac fungus, and the plant diseases are pepper brown spot disease and / or cucumber wilt; or the biological antimicrobial agent is a volatile metabolite of white sac fungus, and the plant diseases are one, two or three of tomato gray mold, pepper brown spot disease and cucumber wilt.

[0013] The experimental results show that white capsule rake tooth fungus and / or its volatile metabolites have a significant inhibitory effect on the growth of tomato gray mold, pepper red spot disease, and cucumber wilt fungus, and can be used to prevent and control tomato gray mold, pepper red spot disease, and cucumber wilt, thereby avoiding the use of pesticides that easily cause pesticide residues and seriously endanger the health of humans and animals.

[0014] The technical solution adopted by the application of the biological antibacterial agent of the present invention in the preparation of a plant disease control agent is:

[0015] The invention discloses an application of a biological antibacterial agent in the preparation of a plant disease control agent, wherein the biological antibacterial agent is a white sac fungus, and the plant disease control agent is used to control pepper brown spot disease and cucumber wilt disease; or the biological antibacterial agent is a volatile metabolite of a white sac fungus, and the plant disease control agent is used to control tomato gray mold disease, pepper brown spot disease and cucumber wilt disease.

[0016] The experimental results show that white capsule rake tooth fungus and / or its volatile metabolites have a significant inhibitory effect on the growth of tomato gray mold, pepper red spot disease, and cucumber wilt fungus, and can be used to prevent and control tomato gray mold, pepper red spot disease, and cucumber wilt, thereby avoiding the use of pesticides that easily cause pesticide residues and seriously endanger the health of humans and animals.

[0017] The technical solution adopted by the plant disease control agent of the present invention is:

[0018] A plant disease control agent comprises a biological antibacterial agent, wherein the biological antibacterial agent is white sac fungus, and the plant disease control agent is used to control pepper brown spot disease and cucumber wilt disease; or the biological antibacterial agent is a volatile metabolite of white sac fungus, and the plant disease control agent is used to control tomato gray mold disease, pepper brown spot disease and cucumber wilt disease.

[0019] The experimental results show that white capsule rake tooth fungus and / or its volatile metabolites have a significant inhibitory effect on the growth of tomato gray mold, pepper red spot disease, and cucumber wilt fungus, and can be used to prepare plant disease control agents for preventing and controlling tomato gray mold, pepper red spot disease, and cucumber wilt, thereby avoiding the use of pesticides that easily cause pesticide residues and seriously endanger the health of humans and animals.

[0020] In the present invention, the classification name of the white capsule rake tooth fungus is white capsule rake tooth fungus (Irpex lacteus) LL210, and the preservation number is CGMCC No.21057; the tomato gray mold fungus is tomato gray mold fungus (Botrytis cinerea Pers); the pepper red star pathogen is pepper red star pathogen (Alternaria alternata); and the cucumber wilt pathogen is cucumber wilt pathogen (Fusarium oxysporum). BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a picture of the bacterial growth when the plate contains only white capsule rake tooth fungus in Experimental Example 4 of the present invention;

[0022] Figure 2 This is a picture of the growth of tomato gray mold bacteria when the plate contains only gray mold bacteria in Experimental Example 4 of the present invention;

[0023] Figure 3 This is a picture of the bacterial growth when the plate contains both white capsule rake tooth fungus and tomato gray mold fungus in Experimental Example 4 of the present invention;

[0024] Figure 4 This is a picture of the bacterial growth when the plate contains only pepper red star pathogen in Experimental Example 4 of the present invention;

[0025] Figure 5 This is a picture of the bacterial growth when the plate contains both white capsule rake tooth fungus and pepper red spot pathogen in Experimental Example 4 of the present invention;

[0026] Figure 6 This is a picture of the bacterial growth when the plate contains only cucumber wilt pathogens in Experimental Example 4 of the present invention;

[0027] Figure 7 This is a picture of the bacterial growth when the plate contains both white capsule rake tooth fungus and cucumber wilt pathogen in Experimental Example 4 of the present invention;

[0028] Figure 8 This is a graph showing the growth of mycelium radius at different times for the experimental group and the control group containing tomato gray mold in Experimental Example 4 of the present invention;

[0029] Fig. 9 This is a graph showing the growth of mycelium radius at different times for the experimental group and the control group containing pepper red spot pathogen in Experimental Example 4 of the present invention;

[0030] Fig.10 This is a graph showing the growth of mycelium radius at different times for the experimental group and the control group containing cucumber wilt pathogen in Experimental Example 4 of the present invention;

[0031] Fig.11 This is a graph showing the growth of mycelium radius of white capsule fungus in the experimental group and the control group at different times in Experimental Example 4 of the present invention. DETAILED DESCRIPTION

[0032] The application of the biological antibacterial agent of the present invention in antagonizing plant pathogenic fungi is a pioneering invention. The experimental results showed that Irpex lacteus LL210 and its volatile metabolites had a significant inhibitory effect on the growth of tomato gray mold (Botrytiscinerea Pers), and Irpex lacteus LL210 and its volatile metabolites can be used to control tomato gray mold; Irpex lacteus LL210 and its volatile metabolites had a significant inhibitory effect on the growth of pepper red spot disease (Alternaria alternata), and Irpex lacteus LL210 and its volatile metabolites can be used to control pepper red spot disease; Irpex lacteus LL210 and its volatile metabolites had a significant inhibitory effect on the growth of cucumber wilt (Fusarium oxysporum), and Irpex lacteus LL210 and its volatile metabolites can be used to control cucumber wilt.

[0033] The invention discloses an application of the white capsule rake tooth fungus and / or its volatile metabolites in antagonizing plant pathogenic fungi, wherein the plant pathogenic fungi are one, two or three of tomato gray mold, pepper brown spot pathogen and cucumber wilt pathogen.

[0034] The invention discloses an application of the white capsule rake tooth fungus and / or its volatile metabolites in preventing and controlling plant diseases, wherein the plant diseases are one, two or three of tomato gray mold, pepper brown spot disease and cucumber wilt.

[0035] The invention discloses an application of the white capsule rake tooth fungus and / or its volatile metabolites in the preparation of a plant disease control agent. The plant disease control agent is used for controlling tomato gray mold, pepper brown spot disease and cucumber wilt.

[0036] A plant disease control agent comprises white capsule fungus and / or its volatile metabolites. The plant disease control agent is used for controlling tomato gray mold, pepper brown spot disease and cucumber wilt.

[0037] The technical solution of the present invention is further described below in conjunction with specific embodiments.

[0038] The use of the biological antibacterial agent (white capsule rake tooth fungus and / or its volatile metabolites) of the present invention in antagonizing plant pathogenic fungi, preventing and controlling plant diseases, and preparing a plant disease control agent, and specific embodiments of the plant disease control agent are described by the following experimental examples:

[0039] Experimental Example 1

[0040] This experimental example is used to verify the antagonistic effect of the volatile metabolites of Irpex lacteus LL210 on tomato gray mold (Botrytis cinerea Pers). The specific experimental method is as follows: tomato gray mold (Botrytis cinerea Pers) is inoculated into a culture dish, and the culture dish is placed on the upper part of the test container, and then the culture medium containing Irpex lacteus LL210 is placed at the lower part of the test container, and then the fan inside the test container is turned on, and the test container is sealed at the same time. After 3 days of cultivation, the inhibition rate of Irpex lacteus on tomato gray mold (Botrytis cinerea Pers) is statistically determined every day. The test time is 11 days in total, and this experiment is named the experimental group experiment. At the same time, a control group experiment is carried out under the same experimental conditions, in which a culture medium without Irpex lacteus LL210 is placed at the lower part of the test container. The calculation method of inhibition rate is as follows: inhibition rate = (the diameter of the colony in the control group experiment - the diameter of the colony in the experimental group experiment) / (the diameter of the colony in the control group experiment - the diameter of the initial bacterial cake) × 100%. The inhibition rate of volatile metabolites of Irpex lacteus LL210 against tomato gray mold (Botrytis cinerea Pers) at different test times is shown in Table 1.

[0041] Table 1 Inhibitory effect of volatile metabolites of Irpex lacteus LL210 on Botrytis cinerea Pers at different test times

[0042] time No. 4d No. 5d 6d No. 7d No. 8d 9d 10d 11d Antibacterial rate 3.05％ 5.37％ 16.36％ 26.94％ 32.04％ 35.51％ 40.84％ 43.78％

[0043] As shown in Table 1, the volatile metabolites of Irpex lacteus LL210 have a significant inhibitory effect on the growth of tomato gray mold (Botrytis cinerea Pers). The volatile metabolites of Irpex lacteus LL210 can be used to control tomato gray mold, and can also be used to prepare plant disease control agents for controlling tomato gray mold.

[0044] Experimental Example 2

[0045] This experimental example is used to verify the antagonistic effect of the volatile metabolites of Irpex lacteus LL210 on pepper red spot pathogen (Alternaria alternata). The specific experimental method is as follows: pepper red spot pathogen (Alternaria alternata) is inoculated into a culture dish, and the culture dish is placed on the upper part of the test container, and then the culture medium containing Irpex lacteus LL210 is placed on the lower part of the test container, and then the fan inside the test container is turned on, and the test container is sealed at the same time. After 3 days of cultivation, the inhibition rate of Irpex lacteus on pepper red spot pathogen (Alternaria alternata) is statistically determined every day, and the test time is 11 days. This experiment is named as the experimental group experiment. At the same time, a control group experiment is carried out under the same experimental conditions, in which a culture medium without Irpex lacteus LL210 is placed on the lower part of the test container. The calculation method of inhibition rate is as follows: inhibition rate = (the diameter of the colony in the control group experiment - the diameter of the colony in the experimental group experiment) / (the diameter of the colony in the control group experiment - the diameter of the initial bacterial cake) × 100%. The inhibition rate of the volatile metabolites of Irpex lacteus LL210 against Alternaria alternata at different test times is shown in Table 2.

[0046] Table 2 Inhibitory effect of volatile metabolites of Irpex lacteus LL210 on Alternaria alternata at different test times

[0047] time No. 4d No. 5d 6d No. 7d No. 8d 9d 10d 11d Antibacterial rate 8.00％ 9.38％ 11.31％ 21.71％ 27.68％ 31.89％ 35.37％ 38.36％

[0048] As shown in Table 2, the volatile metabolites of Irpex lacteus LL210 have a significant inhibitory effect on the growth of Alternaria alternata, and the volatile metabolites of Irpex lacteus LL210 can be used to prevent and control Alternaria alternata, and can also be used to prepare plant disease control agents for preventing and controlling Alternaria alternata.

[0049] Experimental Example 3

[0050] This experimental example is used to verify the antagonistic effect of the volatile metabolites of Irpex lacteus LL210 on the cucumber wilt pathogen (Fusarium oxysporum). The specific experimental method is as follows: the cucumber wilt pathogen (Fusarium oxysporum) is inoculated into a culture dish, and the culture dish is placed on the upper part of the test container, and then the culture medium containing Irpex lacteus LL210 is placed at the lower part of the test container, and then the fan inside the test container is turned on, and the test container is sealed at the same time. After 3 days of cultivation, the inhibition rate of Irpex lacteus on the cucumber wilt pathogen (Fusarium oxysporum) is statistically determined every day, and the test time is 11 days. This experiment is named the experimental group experiment. At the same time, a control group experiment is carried out under the same experimental conditions, in which a culture medium without Irpex lacteus LL210 is placed at the lower part of the test container. The calculation method of inhibition rate is as follows: inhibition rate = (the diameter of the colony in the control group experiment - the diameter of the colony in the experimental group experiment) / (the diameter of the colony in the control group experiment - the diameter of the initial bacterial cake) × 100%. The inhibition rate of the volatile metabolites of Irpex lacteus LL210 against cucumber wilt pathogen (Fusarium oxysporum) at different test times is shown in Table 3.

[0051] Table 3 Inhibitory effect of volatile metabolites of Irpex lacteus LL210 on Fusarium oxysporum at different test times

[0052] time No. 4d No. 5d 6d No. 7d No. 8d 9d 10d 11d Antibacterial rate 5.11％ 6.96％ 7.64％ 12.96％ 12.96％ 12.96％ 12.96％ 12.96％

[0053] As shown in Table 3, the volatile metabolites of Irpex lacteus LL210 have a significant inhibitory effect on the growth of cucumber wilt pathogen (Fusarium oxysporum). The volatile metabolites of Irpex lacteus LL210 can be used to control cucumber wilt disease, and can also be used to prepare plant disease control agents for controlling cucumber wilt disease.

[0054] Experimental Example 4

[0055] The present experimental example is used to verify the inhibitory effect of Irpex lacteus LL210 on Botrytis cinerea Pers, Alternaria alternata and Fusarium oxysporum. The specific experimental method is as follows: a 0.8 cm diameter Irpex lacteus cake is placed on one side of a 9 cm diameter PDA culture medium plate, and a pathogen cake of the same size is placed on the other side, with an interval of 4.5 cm between the two cakes, and the plates are cultured in a constant temperature incubator at 28° C. The growth status is counted on the 1st, 2nd, 3rd, 4th, 5th and 6th day of culture, and the radius of the pathogen cake is recorded, which is recorded as the colony radius of the experimental group; at the same time, a control group 1 and a control group 2 are set, and the control group 1 is No white capsule rake tooth fungus cake was placed on one side of the PDA medium plate, but only the pathogen cake was placed on the other side, and the radius of the pathogen cake was recorded on the 1st, 2nd, 3rd, 4th, 5th, and 6th day of culture, which was recorded as the colony radius of control group 1. In control group 2, white capsule rake tooth fungus cake was placed on one side of the PDA medium plate, but no pathogen cake was placed on the other side, and the radius of the pathogen cake was recorded on the 1st, 2nd, 3rd, 4th, 5th, and 6th day of culture, which was recorded as the colony radius of control group 2. After the experiment, the inhibition rate was calculated. The antibacterial rate on the Xth day = [(the colony radius of the control group on the Xth day - the colony radius of the control group on the X-1th day) - (the colony radius of the experimental group on the Xth day - the colony radius of the experimental group on the X-1th day)] × 100% / (the colony radius of the control group on the Xth day - the colony radius of the control group on the X-1th day). The control group in the antibacterial rate calculation formula refers to the control group 1. The antibacterial rate calculation result is rounded to two decimal places. When X = 1, the colony radius of the experimental group on the X-1th day and the colony radius of the control group on the X-1th day are both 0.4cm. The growth of different bacteria is shown in the figure below. Figure 1-7 As shown, Figure 1 This is a picture of the bacterial growth when the plate only contains white capsule rake tooth fungus. Figure 2 This is a picture of the growth of tomato gray mold bacteria when the plate only contains tomato gray mold bacteria. Figure 3 This is a picture of the bacterial growth when both white capsule rake tooth fungus and tomato gray mold fungus are present in the plate. Figure 4 This is a picture of the growth of the bacteria when the plate only contains red star pathogenic pepper. Figure 5 This is a picture of the bacterial growth when the plate contains both white capsule rake tooth fungus and pepper red star pathogen. Figure 6 This is a picture of the growth of bacteria when the plate only contains cucumber wilt bacteria. Figure 7This is a picture of the bacterial growth when the plate contains both white capsule rake tooth fungus and cucumber wilt pathogenic bacteria. Figure 1 In the figure, “positive” means the photo of the white capsule rake tooth fungus was taken from the front of the culture dish without the PDA culture medium in the middle, and “negative” means the photo of the white capsule rake tooth fungus was taken from the back of the culture dish with the PDA culture medium in the middle; Figure 3 In the figure, “positive” means the photo was taken from the front of the culture dish, with no PDA culture medium in between, and “negative” means the photo was taken from the back of the culture dish, with PDA culture medium in between, and White capsule rake tooth photo antagonistic to tomato gray mold fungus.

[0056] Then the relationship between the radius of different bacteria and time is tabulated and graphed. The results are as follows: Figure 8-11 And as shown in Table 4. Figure 8 are the mycelium radius growth curves of the experimental group and the control group containing Botrytis cinerea at different times, bc represents the mycelium radius growth curve of Botrytis cinerea in the plate when it contains White Capsule Rake Tooth Fungus, and bcck represents the mycelium radius growth curve of Botrytis cinerea in the plate when it does not contain White Capsule Rake Tooth Fungus; Fig. 9 is the mycelial radius growth curve of the experimental group and the control group containing pepper red star pathogen at different times, cx represents the mycelial radius growth curve of pepper red star pathogen in the plate containing white capsule rake tooth fungus, and cxck represents the mycelial radius growth curve of pepper red star pathogen in the plate without white capsule rake tooth fungus; Fig.10 is the mycelium radius growth curve of the experimental group and the control group containing cucumber wilt pathogen at different times, kw represents the mycelium radius growth curve of cucumber wilt pathogen in the plate when white capsule rake tooth fungus is present, and kwck represents the mycelium radius growth curve of cucumber wilt pathogen in the plate when white capsule rake tooth fungus is not present; Fig.11 are the mycelium radius growth curves of White Capsule Rake Tooth Fungus in the experimental group and the control group at different times, ilck represents the mycelium radius growth curve when the plate contains only White Capsule Rake Tooth Fungus, ilbc represents the mycelium radius growth curve of White Capsule Rake Tooth Fungus when the plate contains tomato gray mold, ilcx represents the mycelium radius growth curve of White Capsule Rake Tooth Fungus when the plate contains pepper red spot pathogen, ilkw represents the mycelium radius growth curve of White Capsule Rake Tooth Fungus when the plate contains cucumber wilt pathogen.

[0057] Table 4 Inhibition rate of white capsule rake tooth fungus on tomato gray mold, pepper red star pathogen and cucumber wilt pathogen

[0058]

[0059] The experimental results show that Lrpex lacteus LLC210 has an inhibitory antagonistic effect on tomato gray mold (Botrytiscinerea Pers), pepper red spot pathogen (Alternaria alternata) and cucumber wilt pathogen (Fusarium oxysporum).

Claims

1. The use of biological antimicrobial agents in antagonizing plant pathogenic fungi, characterized in that: The biological antibacterial agent is white sac fungus, and the plant pathogenic fungi are pepper brown mold pathogen and / or cucumber wilt pathogen; or the biological antibacterial agent is the volatile metabolite of white sac fungus, and the plant pathogenic fungi are one, two or three of tomato gray mold pathogen, pepper brown mold pathogen and cucumber wilt pathogen.

2. The application of biological antimicrobial agents in preventing and controlling plant diseases is characterized in that: The biological antibacterial agent is white sac fungus, and the plant diseases are pepper brown spot disease and / or cucumber wilt disease; or the biological antibacterial agent is the volatile metabolites of white sac fungus, and the plant diseases are one, two or three of tomato gray mold disease, pepper brown spot disease and cucumber wilt disease.

3. The use of a biological antimicrobial agent in the preparation of a plant disease control agent, characterized in that: The biological antibacterial agent is white sac fungus, and the plant disease control agent is used to control pepper brown spot disease and cucumber wilt disease; or the biological antibacterial agent is the volatile metabolite of white sac fungus, and the plant disease control agent is used to control tomato gray mold disease, pepper brown spot disease and cucumber wilt disease.

4. A plant disease control agent, characterized in that: The invention comprises a biological antibacterial agent, wherein the biological antibacterial agent is white sac fungus, and the plant disease control agent is used for controlling pepper brown spot disease and cucumber wilt disease; or the biological antibacterial agent is a volatile metabolite of white sac fungus, and the plant disease control agent is used for controlling tomato gray mold disease, pepper brown spot disease and cucumber wilt disease.

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