A Chinese medicine oral liquid for preventing and treating immunosuppressive diseases in livestock and poultry and its preparation method

Abstract

The invention belongs to the field of veterinary Chinese medicine, and provides a Chinese medicine oral liquid for preventing and treating immunosuppressive diseases in livestock and poultry and a preparation method thereof, wherein the Chinese medicine oral liquid is composed of the following components by weight: 22-28 parts of Astragalus, 22-28 parts of Poria, 12-18 parts of Atractylodes macrocephala, 12-18 parts of Scrophularia, 8-12 parts of Cuscuta, 8-12 parts of Viola yedoensis, 8-12 parts of Forsythia, 12-18 parts of Tangerine peel and 4-8 parts of Licorice. The Chinese medicine oral liquid has the effect of enhancing the body's immunity, and experiments show that the Chinese medicine oral liquid of the present invention significantly increases the thymus index and spleen index of immunosuppressive chicks, and increases the bursal index and antibody titer. The dosage form of the present invention is an oral liquid, which can be directly added to livestock and poultry drinking water, is easy to absorb, and has a good market prospect.

Description

Technical Field

[0001] The invention relates to the field of veterinary Chinese medicines, and in particular to a Chinese medicine oral liquid for preventing and treating immunosuppression diseases in livestock and poultry, and a preparation method and application thereof. Background Art

[0002] Immunosuppression refers to the damage to the immune system due to certain factors, which causes temporary or permanent impairment of the immune response function of poultry, leading to increased susceptibility to diseases. There are many factors that cause immunosuppression. According to the cause of the disease and clinical manifestations, immunosuppression can be divided into two categories: primary immunosuppression and secondary immunosuppression.

[0003] Primary immunosuppression is caused by congenital dysplasia, also known as congenital immunodeficiency. Secondary immunosuppression is the immune deficiency caused by various factors such as drugs, nutritional deficiencies, and diseases after the poultry is born.

[0004] Immunosuppression can reduce the effectiveness of vaccine immunity and even lead to immune failure. It can also increase the susceptibility of poultry to diseases. Immunosuppressive diseases often lead to multiple diseases or secondary infections, causing huge economic losses to the poultry industry.

[0005] Currently, the common immunosuppressive diseases in pigs include porcine reproductive and respiratory syndrome, swine fever, porcine circovirus type II, etc., and the common immunosuppressive diseases in chickens include infectious bursal disease, Marek's disease, etc.

[0006] At present, there are few drugs on the market that can enhance the immune function of livestock and poultry. Compared with Western medicine, Chinese medicine does not produce drug resistance, is natural and has no residue, and can also enhance the body's immune function, becoming the first choice for preventing and treating livestock and poultry diseases. As an immunopotentiator, Chinese medicine can enhance the immune effect of various vaccines. As a feed additive, it can also improve the production performance of livestock and poultry, and can regulate the immune function of livestock and poultry to protect livestock and poultry from diseases, thus increasing the profits of the breeding industry and ensuring the quality of livestock products. Therefore, Chinese medicine plays an increasingly important role in animal husbandry production. Summary of the invention

[0007] In order to solve the above technical problems, the present invention provides a Chinese medicinal oral liquid for preventing and treating immunosuppressive diseases in livestock and poultry. The Chinese medicinal oral liquid for preventing and treating immunosuppressive diseases in livestock and poultry is composed of the following components by weight: 22-28 parts of Astragalus, 22-28 parts of Poria, 12-18 parts of Atractylodes, 12-18 parts of Scrophularia, 8-12 parts of Cuscuta, 8-12 parts of Viola yedoensis, 8-12 parts of Forsythia, 12-18 parts of Tangerine peel and 4-8 parts of Licorice.

[0008] In a preferred embodiment of the present invention, the Chinese medicine oral liquid is composed of the following components by weight: 25 parts of Astragalus, 25 parts of Poria, 15 parts of Atractylodes, 15 parts of Scrophularia, 10 parts of Cuscuta, 10 parts of Viola yedoensis, 10 parts of Forsythia, 15 parts of Tangerine Peel and 6 parts of Licorice.

[0009] In a preferred embodiment of the present invention, the Chinese medicine oral liquid is composed of the following components by weight: 22 parts of Astragalus, 22 parts of Poria, 12 parts of Atractylodes, 12 parts of Scrophularia, 8 parts of Cuscuta, 8 parts of Viola yedoensis, 8 parts of Forsythia, 12 parts of Tangerine Peel and 4 parts of Licorice.

[0010] In a preferred embodiment of the present invention, the Chinese medicine oral liquid is composed of the following components by weight: 28 parts of Astragalus, 28 parts of Poria, 18 parts of Atractylodes, 18 parts of Scrophularia, 12 parts of Cuscuta, 12 parts of Viola yedoensis, 12 parts of Forsythia, 18 parts of Tangerine Peel and 8 parts of Licorice.

[0011] The present invention also provides a method for preparing the Chinese medicine oral liquid for preventing and treating immunosuppressive diseases in livestock and poultry, comprising the following steps: weighing each raw medicinal material according to the formula ratio and crushing it; soaking the crushed raw medicinal material in water for 0.5-1h, decocting twice, adding 5-8 times the total amount of medicinal material each time, decocting for 0.5-1.5h each time, combining the decoction, filtering, combining the filtrate, and concentrating to a relative density of 1.05-1.15. In some embodiments, the mass ratio of the cellulase to the ligninase is 2-3:1.

[0012] Furthermore, the preparation method of the Chinese medicine oral liquid for preventing and treating immunosuppression in livestock and poultry comprises the following steps: weighing each raw medicinal material according to the formula ratio and crushing them; soaking the crushed raw medicinal materials in water for 1 hour, decocting them twice, adding 6 times the total amount of water each time, decocting them for 1 hour each time, combining the decoctions, filtering, combining the filtrates, and concentrating them to a relative density of 1.10.

[0013] The present invention also provides application of the Chinese medicinal oral liquid for preventing and treating immunosuppression diseases in livestock and poultry in preparing drugs for preventing and treating immunosuppression diseases in livestock and poultry.

[0014] The invention also provides application of the Chinese medicinal oral liquid for preventing and treating immunosuppression in livestock and poultry in preparing medicine for preventing and treating immunosuppression in chickens.

[0015] Compared with the prior art, the present invention has the following advantages:

[0016] (1) The Chinese medicine oral liquid of the present invention has the effect of enhancing the body's immunity. Clinically, the Chinese medicine oral liquid of the present invention significantly increases the thymus index and spleen index of immunosuppressed chickens, and increases the bursal index and antibody titer.

[0017] (2) The dosage form of the present invention is an oral liquid, which can be directly added to the drinking water of livestock and poultry, is easy to absorb, and has a good market prospect.

[0018] (3) The Chinese medicine oral liquid of the present invention is prepared from natural Chinese herbal medicines and has the advantages of long-lasting efficacy and high safety. It will not cause drug resistance and has high potential development value. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 The effect of the Chinese medicine oral liquid of the present invention on the thymus index of immunosuppressed chicks was compared with the model group. * P<0.05, ** P<0.01; compared with Example 1 group, # P<0.05, ## P<0.01.

[0020] Figure 2 The effect of the Chinese medicine oral liquid of the present invention on the spleen index of immunosuppressed chickens was compared with the model group. * P<0.05, ** P<0.01; compared with the Example 1 group, # P<0.05, ## P<0.01.

[0021] Figure 3 The effect of the oral Chinese medicine liquid of the present invention on the bursal index of immunosuppressed chicks was compared with the model group. * P<0.05, ** P<0.01; compared with Example 1 group, # P<0.05, ## P<0.01.

[0022] Figure 4 The effect of the Chinese medicine oral liquid of the present invention on the antibody titer of immunosuppressed chickens was compared with the model group. * P<0.05, ** P<0.01; compared with Example 1 group, # P<0.05, ## P<0.01. DETAILED DESCRIPTION

[0023] In order to verify the efficacy of the Chinese medicine oral liquid of the present invention, the inventor conducted a pharmacodynamic test study. It should be noted that the drugs selected for the pharmacodynamic test study of the present invention are drugs obtained by the representative formula of the present invention and its preparation method. Due to space limitations, the tests and results involved in the drugs obtained by other formulas and preparation methods included in the present invention are not listed here one by one.

[0024] Example 1: A Chinese medicine oral liquid for preventing and treating immunosuppressive diseases in livestock and poultry, the formula and preparation method are as follows:

[0025] 25 parts of Astragalus, 25 parts of Poria, 15 parts of Atractylodes, 15 parts of Scrophularia, 10 parts of Cuscuta, 10 parts of Viola yedoensis, 10 parts of Forsythia, 15 parts of Tangerine Peel and 6 parts of Licorice.

[0026] The preparation method is as follows:

[0027] Weigh each raw medicinal material according to the formula ratio and crush them; soak the crushed raw medicinal materials in water for 1 hour, boil them twice, add 6 times the total amount of water to the medicinal materials each time, boil them for 1 hour each time, combine the decoctions, filter, combine the filtrates, concentrate to a relative density of 1.10, add potassium sorbate, and the amount of potassium sorbate added is 0.3% of the mass of the supernatant.

[0028] Example 2: A Chinese medicine oral liquid for preventing and treating immunosuppressive diseases in livestock and poultry, the formula and preparation method are as follows:

[0029] 28 parts of Astragalus, 22 parts of Poria, 18 parts of Atractylodes, 12 parts of Scrophularia, 8 parts of Cuscuta, 12 parts of Viola yedoensis, 8 parts of Forsythia, 18 parts of Citrus reticulata and 4 parts of Licorice.

[0030] The preparation method is as follows:

[0031] Weigh each raw medicinal material according to the formula ratio and crush them; soak the crushed raw medicinal materials in water for 0.5h, decoct twice, add 5 times the total amount of water of the medicinal materials each time, decoct for 0.5h each time, combine the decoctions, filter, combine the filtrates, concentrate to a relative density of 1.15, add potassium sorbate, and the amount of potassium sorbate added is 0.3% of the mass of the supernatant.

[0032] Example 3: A Chinese medicine oral liquid for preventing and treating immunosuppression in livestock and poultry, the formula and preparation method are as follows:

[0033] 22 parts of Astragalus, 28 parts of Poria, 12 parts of Atractylodes, 18 parts of Scrophularia, 12 parts of Cuscuta, 8 parts of Viola yedoensis, 12 parts of Forsythia, 12 parts of Tangerine Peel and 8 parts of Licorice.

[0034] The preparation method is as follows:

[0035] Weigh each raw medicinal material according to the formula ratio and crush them; soak the crushed raw medicinal materials in water for 1 hour, decoct twice, each time adding 8 times the total amount of medicinal materials, decoct for 1.5 hours each time, combine the decoctions, filter, combine the filtrates, concentrate to a relative density of 1.05, add potassium sorbate, and the amount of potassium sorbate added is 0.3% of the mass of the supernatant.

[0036] Example 4: A Chinese medicine oral liquid for preventing and treating immunosuppressive diseases in livestock and poultry, the formula and preparation method are as follows:

[0037] 22 parts of Astragalus, 28 parts of Poria, 16 parts of Atractylodes, 12 parts of Scrophularia, 12 parts of Cuscuta, 8 parts of Viola yedoensis, 12 parts of Forsythia, 12 parts of Tangerine Peel and 4 parts of Licorice.

[0038] The preparation method is as follows:

[0039] Weigh each raw medicinal material according to the formula ratio and crush them; soak the crushed raw medicinal materials in water for 1 hour, decoct twice, add 5 times the total amount of water to the medicinal materials each time, decoct for 1.2 hours each time, combine the decoctions, filter, combine the filtrates, concentrate to a relative density of 1.10, add potassium sorbate, and the amount of potassium sorbate added is 0.3% of the mass of the supernatant.

[0040] Example 5: A Chinese medicine oral liquid for preventing and treating immunosuppressive diseases in livestock and poultry, the formula and preparation method are as follows:

[0041] 25 parts of Astragalus, 25 parts of Poria, 14 parts of Atractylodes, 18 parts of Scrophularia, 8 parts of Cuscuta, 10 parts of Viola yedoensis, 10 parts of Forsythia, 18 parts of Citrus reticulata and 7 parts of Licorice.

[0042] The preparation method is as follows:

[0043] Weigh each raw medicinal material according to the formula ratio and crush them; soak the crushed raw medicinal materials in water for 1 hour, boil them twice, add 8 times the total amount of water to the medicinal materials each time, boil them for 1 hour each time, combine the decoctions, filter, combine the filtrates, concentrate to a relative density of 1.08, add potassium sorbate, and the amount of potassium sorbate added is 0.3% of the mass of the supernatant.

[0044] Comparative Example 1: A Chinese medicine oral liquid for preventing and treating immunosuppressive diseases in livestock and poultry, the formula and preparation method are as follows:

[0045] 25 parts of Astragalus, 25 parts of Poria, 15 parts of Atractylodes, 15 parts of Ophiopogon, 10 parts of Cuscuta, 10 parts of Honeysuckle, 10 parts of Forsythia, 15 parts of Tangerine Peel and 6 parts of Licorice.

[0046] The preparation method is the same as Example 1.

[0047] Comparative Example 2: A Chinese medicine oral liquid for preventing and treating immunosuppressive diseases in livestock and poultry, the formula and preparation method are as follows:

[0048] 25 parts of Astragalus, 25 parts of Schisandrae Chinensis, 15 parts of Atractylodes, 15 parts of Scrophularia, 10 parts of Cuscuta, 10 parts of Viola Yen, 10 parts of Fructus Forsythiae, 15 parts of Citrus Reticulatae and 6 parts of Licorice.

[0049] The preparation method is the same as Example 1.

[0050] Comparative Example 3: A Chinese medicine oral liquid for preventing and treating immunosuppression in livestock and poultry, the formula and preparation method are as follows:

[0051] 15 parts of Atractylodes macrocephala, 15 parts of Scrophularia ningpoensis, 10 parts of Cuscuta australis, 10 parts of Viola yedoensis, 10 parts of Fructus Forsythiae, 15 parts of Citrus reticulatae and 6 parts of Radix Glycyrrhizae.

[0052] The preparation method is the same as Example 1.

[0053] Comparative Example 4: A Chinese medicine oral liquid for preventing and treating immunosuppression in livestock and poultry, the formula and preparation method are as follows:

[0054] 25 parts of Astragalus, 25 parts of Poria, 15 parts of Scrophularia, 10 parts of Viola yedoensis, 10 parts of Forsythia, 15 parts of Tangerine Peel and 6 parts of Licorice.

[0055] The preparation method is the same as Example 1.

[0056] Effect Example 1 Effect of the Chinese medicine oral liquid of the present invention on the immune function of chickens

[0057] 1 Experimental methods

[0058] 1.1 Grouping and treatment of experimental animals

[0059] 140 one-day-old Hy-Line brown chicks were selected and randomly divided into Example 1 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group, model group and blank group, with 20 chicks in each group. Except for the blank group, the other groups were injected with an equal amount of cyclophosphamide (80 mg / kg) in the same part starting from the 6th day, once / d, for 4 consecutive days, and the blank group was injected with 0.2 mL of normal saline into the pectoral muscle.

[0060] Starting from the 7th day of chicks, each group was gavaged with corresponding drugs until the 22nd day of age.

[0061] Group 1 of Example 1: 0.3% of the oral Chinese medicine solution of Example 1 was added to drinking water;

[0062] Comparative Example 1 group: 0.3% of the Chinese medicine oral liquid of Comparative Example 1 was added to drinking water;

[0063] Comparative Example 2 group: 0.3% of the Chinese medicine oral liquid of Comparative Example 2 was added to the drinking water;

[0064] Comparative Example 3 group: 0.3% of the Chinese medicine oral liquid of Comparative Example 3 was added to the drinking water;

[0065] Comparative Example 4 group: 0.3% of the Chinese medicine oral liquid of Comparative Example 4 was added to the drinking water;

[0066] The model group and the blank group were fed according to normal feeding management procedures.

[0067] All experimental chickens were fed in the same environment, kept warm by heat lamps, immunized normally, and fed the same feed without any drug additives. The experimental chickens had free access to water and food.

[0068] Ten chicks were randomly selected from each group for sampling on the 15th and 21st days after intragastric administration. Blood was collected from the heart of the chicks using a coagulant tube. After the serum was naturally precipitated, the precipitated serum was grouped, numbered, and packaged, and stored in a -80℃ refrigerator for later use. The chicks were then killed, their thymus, spleen, and bursa of Fabricius were removed, and the weight of the immune organs of each group of chickens was weighed using an analytical balance. Finally, the immune organ index of each group of chicks was calculated.

[0069] 1.3 Indicator Observation

[0070] 1.3.1 Immune organ index determination

[0071] Record the immune organ weight and live body weight, and calculate the immune organ index according to the following formula: immune organ index (g / kg) = immune organ weight / live body weight.

[0072] 1.3.2 Detection of serum ND-HI antibody titer

[0073] The titer of ND antibody in serum was detected by hemagglutination (HA) and hemagglutination inhibition (HI) tests.

[0074] 1.4 Statistical analysis

[0075] SPSS 22.0 software was used for data processing. The data were expressed as mean ± standard deviation. The data of each group were tested for normal distribution and homogeneity of variance, which met the requirements of Pearson's test, and one-way analysis of variance was used for comparison between groups. P < 0.05 was considered statistically significant.

[0076] 2. Experimental results

[0077] 2.1 Effect of the Chinese medicine oral solution of the present invention on the thymus index of immunosuppressed chicks

[0078] like Figure 1 As shown, during the test period, the thymus index of the model group was significantly lower than that of the blank group, indicating that the model was successful. The thymus index of the chicks in the embodiment 1 group was significantly increased, which was significantly higher than that of the comparative example 1-4 groups. The Chinese medicine oral liquid of the present invention has an effect of improving the thymus index of the chicks caused by cyclophosphamide, and the effect is better than that of the comparative example group.

[0079] 2.2 Effect of the Chinese medicine oral solution of the present invention on spleen index of immunosuppressed chickens

[0080] like Figure 2 As shown, the spleen index of the model group was significantly lower than that of the blank group, indicating that the model was successful. The Example 1 group can significantly increase the spleen index of the chicks, which is significantly higher than that of the comparative example 1-4 groups. The Chinese medicine oral liquid of the present invention has an effect of improving the spleen index of the chicks caused by cyclophosphamide, and the effect is better than that of the comparative example group.

[0081] 2.3 Effect of the Chinese herbal oral solution of the present invention on the bursal index of immunosuppressed chicks

[0082] like Figure 3 As shown, the bursal index of the model group was significantly lower than that of the blank group. The bursal index of the chicks in the embodiment 1 group was significantly increased, which was significantly higher than that of the comparative example 1-4 groups. The Chinese medicine oral liquid of the present invention has an effect of improving the bursal index of the chicks caused by cyclophosphamide, and the effect is better than that of the comparative example group.

[0083] 2.4 Effect of the Chinese herbal oral solution of the present invention on antibody titer in immunosuppressed chickens

[0084] like Figure 4 As shown, the antibody titer of the model group was significantly lower than that of the blank group. The Example 1 group can significantly increase the antibody titer of the chicks, which is significantly higher than that of the comparative example 1-4 groups. The Chinese medicine oral liquid of the present invention has an effect of improving the antibody titer of the chicks caused by cyclophosphamide, and the effect is better than that of the comparative example group.

[0085] The Chinese medicinal oral liquid of the invention can significantly increase the thymus index and spleen index of immunosuppressed chickens, increase the bursal index of Fabricius, increase the antibody titer, and enhance the immune function of immunosuppressed chickens.

[0086] The above embodiments are not any form of limitation to the present invention. For those skilled in the art, any modification, replacement, etc. made within the scope of the technical solution of the present invention based on the technical essence of the present invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. A Chinese medicine oral liquid for preventing and treating immunosuppression in livestock and poultry, characterized in that: The Chinese medicine oral liquid is prepared from the following components by weight: 22-28 parts of astragalus, 22-28 parts of poria, 12-18 parts of atractylodes, 12-18 parts of figwort, 8-12 parts of dodder seeds, 8-12 parts of viola yedoensis, 8-12 parts of forsythia, 12-18 parts of tangerine peel and 4-8 parts of liquorice.

2. The Chinese medicine oral liquid for preventing and treating immunosuppressive diseases in livestock and poultry according to claim 1, characterized in that: The Chinese medicine oral liquid is prepared from the following components by weight: 25 parts of astragalus, 25 parts of tuckahoe, 15 parts of atractylodes, 15 parts of scrophularia, 10 parts of dodder seeds, 10 parts of viola yedoensis, 10 parts of forsythia, 15 parts of tangerine peel and 6 parts of liquorice.

3. The Chinese medicine oral liquid for preventing and treating immunosuppressive diseases in livestock and poultry according to claim 1, characterized in that: The Chinese medicine oral liquid is prepared from the following components by weight: 22 parts of astragalus, 22 parts of tuckahoe, 12 parts of atractylodes, 12 parts of scrophularia, 8 parts of dodder seeds, 8 parts of viola yedoensis, 8 parts of forsythia, 12 parts of tangerine peel and 4 parts of liquorice.

4. The Chinese medicinal oral liquid for preventing and treating immunosuppressive diseases in livestock and poultry according to claim 1, characterized in that: The Chinese medicine oral liquid is prepared from the following components by weight: 28 parts of astragalus, 28 parts of tuckahoe, 18 parts of atractylodes, 18 parts of scrophularia, 12 parts of dodder seeds, 12 parts of viola yedoensis, 12 parts of forsythia, 18 parts of tangerine peel and 8 parts of liquorice.

5. A method for preparing a Chinese medicine oral liquid for preventing and treating immunosuppressive diseases in livestock and poultry according to any one of claims 1 to 4, characterized in that: The preparation method comprises the following steps: weighing each raw medicinal material according to the formula ratio and crushing them; soaking the crushed raw medicinal materials in water for 0.5-1h, decocting twice, adding 5-8 times the total amount of water of the medicinal materials each time, decocting for 0.5-1.5h each time, combining the decoctions, filtering, combining the filtrates, and concentrating to a relative density of 1.05-1.

15.

6. The method for preparing the Chinese medicine oral liquid for preventing and treating immunosuppressive diseases in livestock and poultry according to claim 5, characterized in that: The preparation method comprises the following steps: weighing each raw medicinal material according to the formula ratio and crushing them; soaking the crushed raw medicinal materials in water for 1 hour, decocting them twice, adding water 6 times the total amount of the medicinal materials each time, decocting them for 1 hour each time, combining the decoctions, filtering, combining the filtrates, and concentrating them to a relative density of 1.

10.

7. Use of the Chinese medicinal oral liquid for preventing and treating immunosuppression in livestock and poultry as claimed in any one of claims 1 to 4 in the preparation of drugs for preventing and treating immunosuppression in livestock and poultry.

8. Use of the Chinese medicinal oral liquid for preventing and treating immunosuppression in livestock and poultry as claimed in any one of claims 1 to 4 in the preparation of a drug for preventing and treating immunosuppression in chickens.

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