Embryo transplantation tube

Abstract

The invention relates to the field of embryo transplantation, in particular to an embryo transplantation tube. Comprising a tube body, a needle head part is arranged at the far end of the tube body, the needle head part is detachably connected with the tube body, and the needle head part is a tip capable of puncturing; an injection part is arranged at the near-end position of the tube body, the injection part is detachably connected with the tube body, a cotton core part is arranged in the tube body, and the cotton core part can push liquid in the tube body to a needle head part; and the injection part is used for pushing the cotton core part to move. The clone embryo attachment efficiency and the birth rate are improved. Therefore, the efficiency of animal embryo transplantation is improved, and the method plays an important role in animal preservation and high-quality resource protection.

Description

Technical Field

[0001] The present application relates to the field of embryo transplantation, and in particular to an embryo transplantation tube. Background Art

[0002] At present, although there are many embryo transfer devices for small and medium-sized animals, there are few in vitro embryo transfer devices. There are many problems in the embryo transfer process, such as the incomplete in vitro maturation of oocytes of small and medium-sized animals, which leads to low oocyte maturation rate and subsequent embryo development problems. Due to different development conditions, the transplantation position is uncertain. Imperfect embryo transfer devices will cause the embryo to be in different positions in the fallopian tube and uterus, which will affect the implantation of the embryo and finally lead to a low pregnancy rate of the embryo. Summary of the invention

[0003] In order to solve the problems existing in the prior art, the present application provides an embryo transfer tube.

[0004] The embryo transfer tube of the present application is mainly used for small and medium-sized animals, including but not limited to Bama pigs, cats, dogs, etc. When the embryo transfer tube is of a larger size or in some specific use environments, it can also be used for large or medium-large animals.

[0005] The specific technical solutions of this application are as follows:

[0006] 1. An embryo transfer tube, characterized in that it comprises a tube body, a needle head is arranged at the distal end of the tube body, the needle head is detachably connected to the tube body, and the needle head is a sharp tip capable of puncture;

[0007] An injection part is arranged at the proximal end of the tube body, the injection part is detachably connected to the tube body, a cotton core part is arranged inside the tube body, and the cotton core part can push the liquid inside the tube body to the needle head;

[0008] A suction device is also arranged at the proximal end of the tube body, and the suction device is detachably connected to the tube body.

[0009] 2. The embryo transfer tube according to item 1 is characterized in that when the suction device sucks the embryo into the tube body at the proximal end, the embryo can enter the tube body from the distal end of the tube body, and the position of the cotton core in the tube body remains fixed.

[0010] 3. The embryo transfer tube according to item 1 is characterized in that the suction device includes a syringe and a connecting tube fixed to the syringe, the connecting tube has an outer diameter that gradually decreases from the proximal end to the distal end, the connecting tube can be inserted into the tube body, and the tube body and the connecting tube are kept sealed.

[0011] 4. The embryo transfer tube according to item 1 is characterized in that when the embryo is sucked into the tube body using a suction device, the suction of the embryo is stopped after the liquid containing the embryo contacts the cotton core.

[0012] 5. The embryo transfer tube according to item 1 is characterized in that the injection part is used to push the cotton core part to move.

[0013] 6. The embryo transfer tube according to item 1 is characterized in that the cotton core is an air cotton core, and when there are embryo cells inside the tube body, the air cotton core can push the embryo cells out of the tube body.

[0014] 7. The embryo transfer tube according to item 1 is characterized in that the injection part includes a push rod, the push rod is arranged inside the tube body, and the push rod can move back and forth inside the tube body.

[0015] 8. The embryo transfer tube according to item 7 is characterized in that the injection part also includes a fixing part, and the fixing part can be fixed to the proximal end of the tube body.

[0016] 9. The embryo transfer tube according to item 8 is characterized in that the fixing part comprises a fixing ring, which is annular; the inner diameter of the fixing ring is less than or equal to the outer diameter of the tube body, and the fixing ring is connected to the tube body by interference fit.

[0017] 10. The embryo transfer tube according to item 8 is characterized in that the fixing portion includes an interference portion and a fixing ring, the fixing ring is coaxially arranged on the proximal outer side of the interference portion and is integrally formed, and the outer diameter of the interference portion is greater than or equal to the inner diameter of the tube body.

[0018] 11. The embryo transfer tube according to item 9 or 10 is characterized in that a finger cuff is provided on the fixing ring, and two finger cuffs are provided on opposite sides of the fixing ring; the two finger cuffs are symmetrically arranged and both are fixedly connected to the fixing ring.

[0019] 12. The embryo transfer tube according to item 7 is characterized in that a protective plate is provided at the proximal end of the push rod; and the radial dimension of the protective plate is larger than the diameter of the push rod.

[0020] 13. The embryo transfer tube according to item 1 is characterized in that the needle head comprises a sharp injection head and a nesting part, and the nesting part is used to connect the injection head and the tube body.

[0021] 14. The embryo transfer tube according to item 13 is characterized in that the nested portion includes a sleeve plug and a transition tube, the outer diameter of the transition tube is smaller than the inner diameter of the tube body or the inner diameter of the transition tube is larger than the outer diameter of the tube body, the sleeve plug is arranged on the outside of the transition tube, and the sleeve plug can tightly clamp the tube body and the transition tube.

[0022] 15. The embryo transfer tube according to item 14, characterized in that the injection head and the transition tube are integrally formed.

[0023] 16. The embryo transfer tube according to item 14 is characterized in that the sleeve plug includes an elastic portion located at the distal end and a fastening portion at the proximal end, and the elastic portion is conical in shape with a diameter gradually decreasing toward the distal end; and the fastening portion is columnar.

[0024] 17. The embryo transfer tube according to any one of items 1 to 16, characterized in that a heat preservation device is provided on the outside of the tube body, and the heat preservation device is used to keep the tank body warm.

[0025] 18. The embryo transfer tube according to any one of items 1 to 17, characterized in that the needle head includes an injection tube and a one-way tube; the one-way tube is arranged between the tube body and the injection tube.

[0026] 19. The embryo transfer tube according to item 18, characterized in that the needle end of the injection tube is a soft tube.

[0027] 20. The embryo transfer tube according to item 18 is characterized in that the one-way tube can be connected to the tube body and remain sealed, and the one-way tube is fixed on the injection tube.

[0028] Beneficial Effects

[0029] The embryo transfer tube of the present application can make better use of embryos for the imperfection of oocyte in vitro maturation, reduce the risk of embryo transfer, and increase the efficiency of cloned embryo attachment and birth rate. This improves the efficiency of embryo transfer and plays an important role in the preservation of small and medium-sized animals and the protection of high-quality resources. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 It is an exploded view of the embryo transfer tube of this application;

[0031] Figure 2 This is an exploded view of the embryo transfer tube of the present application from another perspective;

[0032] Figure 3 It is an exploded view of the tube body and the suction device of the present application;

[0033] Figure 4 It is a schematic diagram of the structure of the injection tube in this application;

[0034] Figure 5 This is the installation structure diagram of the injection tube and the tube body in this application.

[0035] In the figure, 1. tube body; 2. needle head; 3. injection head; 4. sleeve plug; 5. transition tube; 6. injection part; 7. push rod; 8. fixing ring; 9. finger sleeve; 10. protection plate; 11. air cotton core; 12. syringe; 13. connecting tube; 14. injection tube; 15. intermediate tube. DETAILED DESCRIPTION

[0036] The present application is described in detail below. Although specific embodiments of the present application are shown, it should be understood that the present application can be implemented in various forms and should not be limited by the embodiments described herein. On the contrary, these embodiments are provided to enable a more thorough understanding of the present application and to fully convey the scope of the present application to those skilled in the art.

[0037] It should be noted that certain words are used in the specification and claims to refer to specific components. Those skilled in the art should understand that technicians may use different nouns to refer to the same component. This specification and claims do not use the difference in nouns as a way to distinguish components, but use the functional differences of components as the criterion for distinction. As mentioned throughout the specification and claims, "including" or "comprising" are open-ended terms and should be interpreted as "including but not limited to". The subsequent description of the specification is a preferred embodiment of the present application, but the description is based on the general principles of the specification and is not intended to limit the scope of the present application. The scope of protection of the present application shall be determined by the attached claims.

[0038] refer to Figure 1 and Figure 2 The present application provides an embryo transfer tube. It comprises a tube body 1, a needle head 2 is arranged at the distal end of the tube body 1, the needle head 2 is detachably connected to the tube body 1, and the needle head 2 is a sharp tip capable of puncture;

[0039] An injection part 6 is provided at the proximal end of the tube body 1, and the injection part 6 is detachably connected to the tube body 1. A cotton core part is provided inside the tube body 1, and the cotton core part can push the liquid inside the tube body 1 to the needle head 2;

[0040] refer to Figure 3 A suction device is also provided at the proximal end of the tube body 1 , and the suction device is detachably connected to the tube body 1 .

[0041] The embryo transfer tube is used to transfer embryos. Therefore, when the embryo transfer tube is used, the general steps are suction, storage and injection.

[0042] The tube body 1 is a hollow structure, and the embryo can be temporarily stored in the tube body 1 .

[0043] refer to Figure 1 and Figure 3In the present application, when the embryo transfer tube is used, the suction device is first placed at the proximal end of the tube body 1, and the tube body 1 is connected to the suction device, the tube body 1 is placed in the liquid containing the embryo, and the suction device is used to suck the liquid containing the embryo into the tube body 1. When the liquid is not in contact with the cotton core, air can pass through the cotton core, so that the proximal end of the cotton core is sucked into a negative pressure state by the suction device. At this time, the liquid containing the embryo can be sucked into the tube body 1, until the liquid contacts the cotton core, the liquid will fill the cotton core, and also fill the gap in the cotton core. And the liquid is also blocked by the cotton core. So far, under the dual action of the cotton core and the liquid, the sucked liquid containing the embryo is temporarily stored in the tube body 1, and the suction device cannot suck the liquid. At the same time, the sucked liquid will also exist in the tube body 1, and under the sealing action of the cotton core and the liquid, it will not move inside the tube body 1. Then the suction of the liquid containing the embryo is completed.

[0044] Then, the tube body 1 that has absorbed the embryo liquid is moved to the animal or other location to be injected, and the suction device is replaced with the injection part 6, which is used to push the cotton core.

[0045] refer to Figure 1 and Figure 3 Since the cotton core forms a sealing effect after contacting the liquid, in order to discharge the embryo in the tube body 1, the injection part 6 is used to completely push the cotton core and the liquid containing the embryo at the front end of the cotton core out of the tube body 1. When the injection part 6 pushes the cotton core to move toward the proximal end, the cotton core will continue to push the liquid out of the proximal end of the tube body 1.

[0046] refer to Figure 1 and Figure 3 When it is necessary to inject into an animal or other object, before the injection part 6 pushes the cotton core part, the needle head 2 is installed on the proximal end of the tube body 1, and then the tip of the needle head 2 is inserted into the target position, and then the injection part 6 is used to expel the embryo from the tube body 1, and then the embryo is transported to the target position through the needle head 2. In this way, the embryo transplantation is completed.

[0047] refer to Figure 1 and Figure 3 In the present application, a cotton core is arranged in the tube body 1, so that the liquid containing the embryo stops after being sucked into the position of the cotton core in the tube body 1. In this way, on the one hand, the cotton core can control the amount of embryo or liquid sucked into the tube body 1 by moving its position; on the other hand, after the cotton core contacts the liquid, it seals the tube body 1, so that the liquid sucked into the tube body 1 is also sealed into the tube body 1, reducing the possibility of the embryo falling out of the tube body 1.

[0048] refer to Figure 1 and Figure 3The present application adds a suction device, so that the tube body 1 can suck the embryo into the tube body 1 under the action of the suction device. Compared with injecting or delivering the embryo into the tube body 1, the sucked embryo is less likely to be damaged. In addition, the sucked embryo is more easily controlled by the suction device inside the tube body 1, which facilitates placing the embryo in a suitable position.

[0049] The embryo sucked into the tube body 1 is pushed out of the tube body 1 by the injection part 6 , and the injection part 6 pushes the cotton core part to expel the cotton core part and the embryo displaced at its proximal end from the tube body 1 , thereby reducing the embryo residue in the tube body 1 .

[0050] refer to Figure 1 and Figure 3 The needle head 2 is fixed at the front end of the tube body 1, and the needle head 2 is used to pierce the animal or other objects, so that the tube body 1 can be directly injected under the action of the needle head 2, simplifying the cumbersome steps of sucking the embryo inside the tube body 1 with the syringe 12 and then injecting it; the efficiency of embryo transplantation from the tube body 1 is greatly increased. At the same time, the possibility of embryo damage during the transplantation process can also be reduced.

[0051] refer to Figure 1 and Figure 3 When the suction device sucks the embryo into the tube body 1 at the proximal end, the embryo can enter the tube body 1 from the distal end of the tube body 1, and the position of the cotton core in the tube body 1 remains fixed.

[0052] refer to Figure 3 The suction device is a negative pressure suction device, and the cotton core part is a structure with a gap in the middle through which gas can pass. Therefore, when the suction device is sucking, the suction device is in a negative pressure device, where air can pass through the gap in the middle of the cotton core part, and then the liquid containing the embryo can be sucked into the tube body 1 by the negative pressure device while the cotton core part remains stationary.

[0053] At the same time, the cotton core also has a capillary structure. When the absorbed liquid containing the embryo comes into contact with the cotton core, the liquid will penetrate into the cotton core and fill the cotton core, so that a sealing structure is formed between the cotton core and the tube body 1, thereby allowing the liquid containing the embryo to remain in the tube body 1 and stay at the cotton core.

[0054] refer to Figure 3 The suction device includes a syringe 12 and a connecting tube 13 fixed to the syringe 12. The connecting tube 13 has an outer diameter that gradually decreases from the proximal end to the distal end. The connecting tube 13 can be inserted into the tube body 1, and the tube body 1 and the connecting tube 13 are kept sealed.

[0055] The syringe 12 is a conventional syringe 12 in the art. The connecting tube 135 is used to connect the syringe 12 with the tube body 1, the proximal end of the connecting tube 13 is fixedly connected to the distal end of the syringe 12, and the connecting tube 13 and the syringe 12 are sealed. The syringe 12 can form a negative pressure state at the proximal end of the tube body 1.

[0056] refer to Figure 3 The distal end of the connecting tube 13 is used to connect with the tubular body 1, and the syringe 12 is used to extract the air inside the tubular body 1. Therefore, the sealing between the connecting tube 13 and the tubular body 1 needs to be maintained.

[0057] refer to Figure 3 In the present application, the distal end of the connecting tube 13 is set to have a gradually decreasing diameter from the proximal end to the distal end. The distal end of the connecting tube 13 can gradually enter the interior of the tube body 1, thereby achieving a fit and seal between the outer wall of the connecting tube 13 and the inner wall of the tube body 1. When the syringe 12 is sucking, in order to reduce the possibility of air leakage between the connecting tube 13 and the tube body 1, the connecting tube 13 can be further inserted into the tube body 1, so that an interference fit is formed between the tube body 1 and the connecting tube 13. This allows the syringe 12 to more smoothly suck the embryo into the tube body 1.

[0058] refer to Figure 3 When the embryo is sucked into the tube body 1 using the suction device, the embryo is stopped from being sucked after the liquid containing the embryo contacts the cotton core.

[0059] refer to Figure 3 When the embryo is sucked into the tube body 1, the liquid containing the embryo will continue to move toward the proximal direction inside the tube body 1 as the suction device sucks until it contacts the cotton core. Then, the liquid will penetrate into the cotton core, filling the cotton core with liquid, thereby forming a seal between the cotton core and the tube body 1. At this time, the suction device sucks the tube body 1 again. Since the liquid in the cotton core has been sealed by the tube body 1, it is difficult for the liquid in the cotton core to move in the cotton core. Therefore, the suction device cannot suck the liquid again after the cotton core is sealed. At this point, the suction of the embryo is completed.

[0060] refer to Figure 1 and Figure 2 The injection part 6 is used to push the cotton core part to move.

[0061] After the embryo is sucked into the tube body 1, the tube body 1 is moved to the position where the injection is required, and the embryo can be pushed out of the tube body 1 using the injection part 6. Since the cotton core inside the tube body 1 seals the tube body 1, the injection part 6 directly pushes the cotton core, and the cotton core will continue to push the liquid at its proximal position and the embryo inside, thereby completely pushing the embryo inside the tube body 1 out of the tube body 1.

[0062] refer to Figure 1 and Figure 2 The cotton core part is an air cotton core 11. When there are embryonic cells inside the tube body 1, the air cotton core 11 can push the embryonic cells out of the tube body 1.

[0063] refer to Figure 1 and Figure 2 The air cotton core 11 is the cotton core used in the cryopreservation tube in the art. There are gaps inside the air cotton core 11 so that gas can pass through the air cotton core 11.

[0064] In addition, the gaps inside the air cotton core 11 can allow air to pass through, but can also block the flow of liquid. When the liquid flows to the position of the air cotton core 11, the gaps inside the air cotton core 11 will be absorbed into the air cotton core 11 under the action of capillary phenomenon.

[0065] refer to Figure 1 and Figure 2 Therefore, when embryonic cells and liquid are stored inside the tube body 1, the air cotton core 11 will move toward the proximal end, and then the air inside the tube body 1 will be discharged to the proximal end of the air cotton core 11, until the air cotton core 11 contacts the embryonic cells and liquid, and the liquid fills the gap in the air cotton core 11, so that a seal is formed between the air cotton core 11 and the tube body 1, and then when the air cotton core 11 is pushed, the air cotton core 11 in a sealed state with the tube body 1 will simultaneously push the embryonic cells inside the tube body 1 to move. And because the air cotton core 11 is made of a relatively soft material and uses liquid for sealing, the damage caused to the embryonic cells when the air cotton core 1 contacts them is greatly reduced. Therefore, the embryonic cells inside the tube body 1 can ensure sufficient integrity, reducing the possibility of damage to the embryo during transplantation.

[0066] refer to Figure 1 and Figure 2 The injection part 6 includes a push rod 7, which is arranged inside the tube body 1 and can move back and forth inside the tube body 1.

[0067] The push rod 7 is a cylindrical structure, the push rod 7 is located inside the tube body 1, and the length of the push rod 7 is adapted to the length of the tube body 1. Preferably, the length of the push rod 7 is slightly smaller than the length of the tube body 1.

[0068] When the air cotton core 11 needs to be pushed, the push rod 7 can be placed inside the tube body 1 at the proximal end of the tube body 1, and the air cotton core 11 can be pushed by pushing the push rod 7. The air cotton core 11 can push the embryonic cells and liquid inside the tube body 1 out of the tube body 1.

[0069] refer to Figure 1 and Figure 2The injection part 6 also includes a fixing part, which can be fixed to the proximal end of the tube body 1.

[0070] refer to Figure 1 and Figure 2 The fixing part is arranged at the proximal end of the tube body 1, close to the position of the operator. On the one hand, the fixing part can be fixed on the tube body 1. When the push rod 7 is used to push the air cotton core 11 inside the tube body 1, the fixing part can fix the tube body 1, thereby reducing the possibility that the tube body 1 moves synchronously with the air cotton core 11 when the push rod 7 pushes the air cotton core 11, so that the push rod 7 can push the air cotton core 11 to move inside the tube body 1.

[0071] On the other hand, the operator can also use the fixed part to gain leverage. When the operator pushes the push rod 7, the air cotton core 11 and the tube body 1 are in a sealed state, and embryonic cells and liquid are present inside the tube body 1. Therefore, a certain amount of power is required to push the air cotton core 11. Therefore, when the operator finds it difficult to push the push rod 7 to move the air cotton core 11, the operator can use his fingers or other components to act on the fixed part to partially disperse the force of pushing the push rod 7 to the fixed part, so that the fixed part can provide a certain pulling force for the push rod 7, thereby enabling the operator to use the push rod 7 to push the air cotton core 11 more conveniently.

[0072] refer to Figure 1 and Figure 2 The fixing part includes a fixing ring 8, which is annular; the inner diameter of the fixing ring 8 is less than or equal to the outer diameter of the tube body 1, and the fixing ring 8 is connected to the tube body 1 by interference fit.

[0073] refer to Figure 1 and Figure 2 The fixing ring 8 is used to fix with the pipe body 1, wherein when the fixing ring 8 and the pipe body 1 are in interference fit, the inner diameter of the fixing ring 8 can be slightly larger than the outer diameter of the pipe body 1, so that the pipe body 1 or the fixing ring 8 can undergo a certain elastic deformation, thereby making the fixing ring 8 sleeved and fixed on the outside of the pipe body 1. Alternatively, the fixing ring 8 includes a stepped interference portion; the inner diameter of the pipe body 1 is slightly smaller than the outer diameter of the connection position of the fixing ring 8 and the pipe body 1, that is, the outer diameter of the interference portion is larger than the inner diameter of the pipe body 1, thereby making the pipe body 1 sleeved on the outside of the interference portion.

[0074] refer to Figure 1 and Figure 2 The tube body 1 and the fixing ring 8 are both made of a material with a certain elasticity, such as plastic or polyethylene. Therefore, the interference fit between the fixing ring 8 and the tube body 1 can not only realize the fixed connection between the tube body 1 and the fixing ring 8, but also within a certain elastic range, the fixing ring 8 can be removed from the tube body 1, thus realizing a detachable connection between the tube body 1 and the fixing ring 8.

[0075] In a specific embodiment, reference Figure 1 and Figure 2 The fixing part includes an interference part (not shown in the figure) and a fixing ring 8, the fixing ring 8 is coaxially arranged outside the proximal end of the interference part and is integrally formed, and the outer diameter of the interference part is greater than or equal to the inner diameter of the tube body 1. At this time, the tube body 1 is sleeved outside the interference part.

[0076] refer to Figure 1 and Figure 2 In another specific embodiment, the fixing portion includes a fixing ring 8. At this time, the fixing ring 8 is sleeved on the outside of the tube body 1.

[0077] refer to Figure 1 and Figure 2 A finger sleeve 9 is arranged on the fixing ring 8 , and two finger sleeves 9 are arranged on opposite sides of the fixing ring 8 ; the two finger sleeves 9 are symmetrically arranged and are both fixedly connected to the fixing ring 8 .

[0078] refer to Figure 1 and Figure 2 The finger sleeve 9 is fixed on the fixing ring 8. When the fixing ring 8 is installed on the tube body 1, the finger sleeve 9 is also fixedly connected to the tube body 1. Therefore, when pushing the push rod 7, the operator can put his finger in the finger sleeve 9 and use the finger sleeve 9 to apply the force of pushing the push rod 7 to the fixing ring 8. It is more convenient for the operator to apply force to the push rod 7. At the same time, after the operator puts his finger into the finger sleeve 9, the tube body can be moved and fixed by the finger sleeve 9, and then the tube body is moved to a convenient position and fixed. Improve the convenience of the tube body 1 and embryo transplantation.

[0079] refer to Figure 1 and Figure 2 A protective plate 10 is provided at the proximal end of the push rod 7 ; the radial dimension of the protective plate 10 is larger than the diameter of the push rod 7 .

[0080] The protection plate 10 is plate-shaped, and the protection plate 10 can be circular, square, triangular or other shapes, preferably circular.

[0081] refer to Figure 1 and Figure 2 When the operator uses the push rod 7 to push the air cotton core 11 , the operator can press the proximal end of the push rod 7 with a finger, thereby pushing the push rod 7 to move inside the tube body 1 .

[0082] refer to Figure 1 and Figure 2 The diameter of the protection plate 10 is larger than the diameter of the push rod 7, and at the same time, the surface area of ​​the protection plate 10 is larger than the cross-sectional area of ​​the push rod 7, thereby increasing the contact area between the human hand and the protection plate 10, thereby reducing the pressure on the operator's fingers and reducing the damage to the operator's hands.

[0083] In addition, the radial dimension of the protection plate 10 is larger than the radial dimension of the tube body 1. When the push rod 7 is used to push the air cotton core 11, the protection plate 10 can prevent the push rod 7 from entering the inside of the tube body 1 as a whole. Therefore, after the push rod 7 pushes the air cotton core 11, it can be smoothly removed from the tube body 1, reducing the possibility that the push rod 7 completely enters the tube body 1 and is difficult to remove.

[0084] refer to Figure 1 and Figure 2 The needle head 2 includes a sharp injection head 3 and a nesting portion, and the nesting portion is used to connect the injection head 3 and the tube body 1.

[0085] refer to Figure 1 and Figure 2 The injection head 3 is used for puncturing and injection. When it is necessary to inject an embryo into an animal, the injection head 3 can pierce the skin and enter the target position in the animal, and then the embryo inside the tube body 1 can be injected into the animal.

[0086] The sharp portion of the injection head 3 is used to pierce the skin and internal tissues of the animal, thereby delivering the tip of the injection head 3 to the target position in the animal's body.

[0087] refer to Figure 1 and Figure 2 The nesting part is used to fix the injection head 3 to the tube body 1. The injection head 3 is a hollow cylindrical shape, and the outer diameter of the injection head is slightly smaller than the inner diameter of the tube body. Therefore, when the injection head 3 and the tube body are installed, the tube body is sleeved on the outside of the injection head 3, and the injection head 3 and the tube body are interference fit to achieve the connection between the injection head 3 and the tube body.

[0088] refer to Figure 1 and Figure 2 When the injection head 3 is used for injection, in order to improve the connection strength and sealing effect between the injection head 3 and the tube body, the nesting part is moved to the connection between the injection head 3 and the tube body, and then the injection head 3 and the tube body are clamped by the nesting part to improve the connection strength between the tube body and the injection head 3, and at the same time, the sealing between the tube body and the injection head 3 can be improved, thereby reducing the possibility of liquid leakage from the connection between the tube body and the injection head 3 when injecting embryos into the animal body. Improve the success rate of embryo transplantation.

[0089] refer to Figure 1 and Figure 2 In a specific embodiment, the nested portion includes a sleeve plug 4 and a transition pipe 5, the outer diameter of the transition pipe 5 is smaller than the inner diameter of the tube body 1 or the inner diameter of the transition pipe 5 is larger than the outer diameter of the tube body 1, and the sleeve plug 4 is arranged on the outside of the transition pipe 5, and the sleeve plug 4 can tightly clamp the tube body 1 and the transition pipe 5.

[0090] refer to Figure 1 and Figure 2 When the size of the injection head 3 used is difficult to adapt to the size of the tube body 1, for example, a smaller diameter injection head 3 is used for a smaller animal, or a larger diameter injection head 3 is used for embryo transplantation of a larger animal. At this time, it is difficult to directly connect the injection head 3 and the tube body. At this time, a transition tube 5 can be used to connect the injection head 3 and the tube body respectively, thereby realizing the connection between the injection head 3 and the tube body.

[0091] The size of the transition pipe 5 is adapted to the size of the injection head 3 , so that the transition pipe 5 can be connected to the injection head 3 and the connection strength and sealing between the transition pipe 5 and the injection head 3 are maintained.

[0092] refer to Figure 1 and Figure 2 After the transition tube 5 is connected to the injection head 3, it is connected to the tube body. At this time, after the transition tube 5 that matches the size of the injection head 3 is connected to the tube body, the sleeve plug 4 is used to reinforce the tube body and the transition tube 5. The sleeve plug 4 can cause elastic deformation between the tube body or the transition tube 5, thereby strengthening the strength between the tube body and the transition tube 5; at the same time, it can also increase the sealing between the tube body and the transition tube 5, and reduce the possibility of leakage between the liquid and the tube body and the transition tube 5.

[0093] refer to Figure 1 and Figure 2 In a specific embodiment, the injection head 3 and the transition pipe 5 are integrally formed.

[0094] The injection head 3 and the transition pipe 5 are integrally formed, thereby strengthening the connection strength between the transition pipe 5 and the injection head 3 and further improving the sealing between the injection head 3 and the transition pipe 5 .

[0095] refer to Figure 1 and Figure 2 The sleeve plug 4 includes an elastic portion at the distal end and a fastening portion at the proximal end. The elastic portion is conical in shape with a diameter gradually decreasing toward the distal end; the fastening portion is columnar.

[0096] refer to Figure 1 and Figure 2 The sleeve plug 4 is used to connect the transition pipe 5 and the pipe body 1. Therefore, after the sleeve plug 4 is sleeved on the pipe body 1 and the transition pipe 5, the sleeve plug 4 can tighten the pipe body 1 or the transition pipe 5, and make the pipe body 1 or the transition pipe 5 undergo a certain amount of elastic deformation inward, thereby making the transition pipe 5 and the pipe body 1 in close contact to complete the sealing between the transition pipe 5 and the pipe body 1.

[0097] refer to Figure 1 and Figure 2The fastening portion is cylindrical, and the inner diameter of the fastening portion is smaller than the outer diameter of the transition pipe 5 or the pipe body 1 , so the fastening portion can achieve a tightening and sealing connection between the transition pipe 5 and the pipe body 1 .

[0098] refer to Figure 1 and Figure 2 The elastic part is a part with greater elasticity, and the elastic part and the fastening part are integrally formed, so when the sleeve plug 4 is inserted into the pipe body 1 and the transition pipe 5, the elastic part can be stretched to increase the diameter of the elastic part, and then the elastic part and the fastening part are sleeved onto the pipe body 1 and the transition pipe 5, thereby reducing the difficulty of installing the sleeve plug 4.

[0099] refer to Figure 4 and Figure 5 In another specific embodiment, the needle head 2 includes an injection tube 14 and a one-way tube 15 ; the one-way tube 15 is arranged between the tube body 1 and the injection tube 14 .

[0100] In another specific embodiment, an intermediate tube is arranged between the injection tube 14 and the tube body 1, and the intermediate tube is fixedly connected to the injection tube 14. The side of the intermediate tube close to the tube body 1 is a central opening and has an elastic structure, so that the intermediate tube can be connected to tube bodies 1 of various sizes, thereby improving the applicability of the intermediate tube.

[0101] refer to Figure 4 and Figure 5 The injection tube 14 is used to be inserted into the animal body, and then the embryo in the tube body 1 is injected into the target position in the animal body through the injection tube 14.

[0102] refer to Figure 4 and Figure 5 The one-way tube 15 is used to connect the injection tube 14 and the tube body 1. The one-way tube 15 is a pipe that can only allow liquid to flow from the tube body 1 to the injection tube 14, so that the liquid in the injection tube 14 cannot flow into the tube body 1 through the one-way tube 15, thereby reducing the possibility of blood return or backflow when the injection tube 14 is in the animal body. The safety of the tube body 1 using the injection tube 14 to inject liquid into the animal body is improved.

[0103] refer to Figure 4 and Figure 5 In a specific embodiment, the injection tube 14 is an indwelling needle. After the injection tube 14 is inserted into the animal body, the injection tube 14 can be temporarily retained in the animal body. Thus, the surgeon can use the injection tube 14 to inject other substances into the animal body after the embryo or liquid is injected into the tube body 1. This improves the error tolerance during the operation and thus achieves the purpose of improving the success rate of the operation.

[0104] refer to Figure 4 and Figure 5The needle end of the injection tube 14 is a soft tube.

[0105] The needle end of the injection tube 14 is the part that is inserted into the animal's body, and the needle end is set as a hose, so that after the needle end is in the animal's body, the damage to the animal's body caused by the needle end as the animal moves can be reduced. Therefore, the hose-style needle end can be temporarily retained in the animal's body, and has little impact on the animal's movement and daily life. When it is necessary to continue injecting drugs or other substances into the animal's body, the injection tube 14 can be used directly for injection, reducing multiple punctures of the animal, thereby reducing the possibility of excessive struggle of the animal during the operation.

[0106] refer to Figure 4 and Figure 5 It is worth mentioning that when injecting embryos into animals, the injection tube 14 is inserted into the oviduct of the animal, and then the embryo is injected into it. The oviduct of the animal is extremely fragile, so the needle end is set to a hose structure, thereby reducing the damage of the injection tube 14 to the oviduct of the animal, thereby reducing the harm to the animal and improving the success rate of embryo transplantation.

[0107] refer to Figure 4 and Figure 5 The one-way tube 15 can be connected to the tube body 1 and remain sealed, and the one-way tube 15 is fixed on the injection tube 14.

[0108] refer to Figure 4 and Figure 5 The one-way tube 15 is used to connect the injection tube 14 with the tube body 1. In different usage environments, the size of the tube body 1 may change, making it difficult to connect the tube body 1 and the injection tube 14 during installation. Therefore, the one-way tube 15 is used to connect the tube body 1 and the injection tube 14. One side of the one-way tube 15 is fixedly connected to the injection tube 14. The side of the one-way tube 15 close to the tube body 1 is an elastic tube, and the diameter of the one-way tube 15 gradually decreases from the end to the middle. Therefore, the one-way tube 15 can be connected to tube bodies 1 of more sizes, and tube bodies 1 of various sizes can enter the interior along the tapered end of the one-way tube 15 until a sealed and fixed connection is achieved with the one-way tube 15.

[0109] refer to Figure 1 and Figure 5 A heat preservation device (not shown in the figure) is arranged on the outside of the tube body 1, and the heat preservation device is used to keep the tank body warm.

[0110] The heat preservation device is used to keep the tube body 1 warm. During the embryo transplantation operation, the embryos are cultured inside the operating room, but the animals that need embryo transplantation do not live in the operating room. Therefore, after the embryo cells are absorbed by the tube body 1, the tube body 1 will be moved to the position where the animal is fixed, and then injected into the animal. During the movement of the tube body 1 containing the embryo liquid, in order to reduce the impact of the external environment on the survival of embryo cells and improve the survival rate of the embryo, a heat preservation device is used to keep the embryo at a suitable survival temperature when it moves in the tube body 1. This reduces the death of embryo cells caused by temperature increase or decrease. Improve the success rate of embryo transplantation operations.

[0111] In one specific embodiment, the heat preservation device is a thermostat. The thermostat is used to control the temperature of the tube body 1. When the tube body 1 is moved, the tube body 1 is directly placed in the thermostat, and the thermostat is used to isolate the tube body 1 from the external environment, thereby reducing the impact of the external environment on the embryo in the tube body 1.

[0112] In summary, the present application provides an embryo transplantation needle. When performing an embryo transplantation operation, it is necessary to use an aspiration device to absorb the liquid containing the embryo into the tube body 1. First, place the air cotton core 11 inside the tube body 1, and place the air cotton core 11 in a preset fixed position according to actual conditions. The position of the air cotton core 11 is related to the liquid volume of the embryo to be absorbed and the number of embryonic cells. Then use the connecting tube 13 to connect to the tube body 1, and at the same time ensure that the syringe 12 is fixedly connected to the connecting tube 13. At this time, the needle head 2 can be connected to the tube body 1 and the needle head 2 can be used to absorb the embryo, or the tube body 1 can be directly used to absorb the embryo. Put the needle head 2 into the liquid containing the embryo, and then use the syringe 12 to absorb it, so that the liquid containing the embryo will be sucked into the tube body 1. Until the liquid contacts the air cotton core 11, and then under the capillary phenomenon of the air cotton core 11, the liquid quickly penetrates the air cotton core 11, and then the air cotton core 11 and the tube body 1 are sealed. At this time, because the soaked air cotton core 11 blocks one end of the tube body 1, under the action of atmospheric pressure, the embryonic cells and liquid absorbed inside the tube body 1 will also stay in the tube body 1. Then the connecting tube 13 and the syringe 12 are removed, and the tube body 1 is placed in an incubator for heat preservation. At the same time, the operator takes the incubator and the tube body 1 inside it to the position of the animal where the embryo is required.

[0113] When the embryo is transplanted into the animal, the needle head 2 is first installed on the tube body 1. Then the injection part 6 is installed at the proximal position of the tube body 1, the fixing ring 8 is fixed to the tube body 1, and the push rod 7 is abutted against the air cotton core 11. Then the needle head 2 is used to pierce into the animal body, so that the tip of the needle head 2 reaches the target position in the animal body. Then the operator can put his finger into the finger sleeve 9 of the injection part 6, and use the push rod 7 to push the air cotton core 11, and then use the air cotton core 11 to push the embryo inside the tube body 1 into the animal body. Then the embryo transplantation operation is completed.

[0114] When the needle head 2 is installed, when the needle head is an injection head 3, the injection head 3 is connected to the tube body 1 using the nesting part, and the injection head 3 and the tube body 1 are tightened and fixed.

[0115] When the needle head 2 is a syringe 14, the needle end of the syringe 14 is a soft tube, so an auxiliary rigid needle is needed to puncture the animal skin or tissue, and the needle of the syringe 14 is transported to the target position, and then the auxiliary rigid needle is withdrawn to complete the insertion of the syringe 14.

[0116] Example

[0117] The present application provides an embryo transfer tube, wherein the tube body 1 is an embryo transfer tube with a length of 8 to 12 cm and a width of 1.9 to 2 mm; the injection head 3 is an injection head 3 selected to be smaller than the size of the fallopian tube but larger than the early embryo; preferably, the needle of the injection head 3 is a 0.9 mm*37 mm needle. The transition tube 5 and the injection head 3 are an integrally formed structure. The sleeve plug 4 is used to fasten the transition tube 5 and the tube body 1.

[0118] In this embodiment, the experimental subject is a Bama pig. The Bama pig is used for embryo transplantation, and the cultured embryonic cells are transplanted into the Bama pig.

[0119] The air cotton core 11 used in the cotton core part is the air cotton core 11 inside the French caso beef jelly fine tube.

[0120] The suction device is a syringe 12, and specifically, the suction device is a 1 ml syringe 12. The connector is made of plastic.

[0121] The heat preservation device is a constant temperature box.

[0122] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. An embryo transfer tube, characterized in that: It comprises a tube body, a needle head is arranged at the distal end of the tube body, the needle head is detachably connected to the tube body, and the needle head is a sharp tip capable of puncture; An injection part is arranged at the proximal end of the tube body, the injection part is detachably connected to the tube body, a cotton core part is arranged inside the tube body, and the cotton core part can push the liquid inside the tube body to the needle head; A suction device is also arranged at the proximal end of the tube body, and the suction device is detachably connected to the tube body.

2. The embryo transfer tube according to claim 1, characterized in that: When the suction device sucks the embryo into the tube body at the proximal end, the embryo can enter the tube body from the distal end of the tube body, and the position of the cotton core in the tube body remains fixed.

3. The embryo transfer tube according to claim 1, characterized in that: The suction device comprises a syringe and a connecting tube fixed to the syringe, wherein the outer diameter of the connecting tube gradually decreases from the proximal end to the distal end, the connecting tube can be inserted into the tube body, and the tube body and the connecting tube are kept sealed.

4. The embryo transfer tube according to claim 1, characterized in that: When the embryo is sucked into the tube body by using the suction device, the suction of the embryo is stopped after the liquid containing the embryo contacts the cotton core.

5. The embryo transfer tube according to claim 1, characterized in that: The injection part is used to push the cotton core part to move.

6. The embryo transfer tube according to claim 1, characterized in that: The cotton core part is an air cotton core. When embryonic cells are stored in the tube body, the air cotton core can push the embryonic cells out of the tube body.

7. The embryo transfer tube according to claim 1, characterized in that: The injection part comprises a push rod, which is arranged inside the tube body and can reciprocate inside the tube body.

8. The embryo transfer tube according to claim 7, characterized in that: The injection part further includes a fixing part, and the fixing part can be fixed to the proximal end of the tube body.

9. The embryo transfer tube according to claim 8, characterized in that: The fixing part comprises a fixing ring, which is annular; the inner diameter of the fixing ring is smaller than or equal to the outer diameter of the tube body, and the fixing ring is connected to the tube body by interference fit.

10. The embryo transfer tube according to claim 8, characterized in that: The fixing portion comprises an interference portion and a fixing ring, wherein the fixing ring is coaxially arranged outside the proximal end of the interference portion and is integrally formed, and the outer diameter of the interference portion is greater than or equal to the inner diameter of the tube body.

11. The embryo transfer tube according to claim 9, characterized in that: A finger sleeve is arranged on the fixing ring, and two finger sleeves are arranged on two opposite sides of the fixing ring; the two finger sleeves are symmetrically arranged and both are fixedly connected to the fixing ring.

12. The embryo transfer tube according to claim 7, characterized in that: A protection plate is arranged at the proximal end of the push rod; the radial dimension of the protection plate is larger than the diameter of the push rod.

13. The embryo transfer tube according to claim 1, characterized in that: The needle head comprises a sharp injection head and a nesting part, and the nesting part is used for connecting the injection head and the tube body.

14. The embryo transfer tube according to claim 13, characterized in that: The nested portion includes a sleeve plug and a transition pipe, the outer diameter of the transition pipe is smaller than the inner diameter of the pipe body or the inner diameter of the transition pipe is larger than the outer diameter of the pipe body, the sleeve plug is arranged on the outside of the transition pipe, and the sleeve plug can tightly clamp the pipe body and the transition pipe.

15. The embryo transfer tube according to claim 14, characterized in that: The injection head and the transition pipe are integrally formed.

16. The embryo transfer tube according to claim 14, characterized in that: The sleeve plug comprises an elastic portion at a distal end and a fastening portion at a proximal end, wherein the elastic portion is in a cone shape with a diameter gradually decreasing toward the distal end; and the fastening portion is in a column shape.

17. The embryo transfer tube according to any one of claims 1 to 16, characterized in that: A heat preservation device is arranged outside the tube body, and the heat preservation device is used to keep the tank body warm.

18. The embryo transfer tube according to any one of claims 1 to 16, characterized in that: The needle head comprises an injection tube and a one-way tube; the one-way tube is arranged between the tube body and the injection tube.

19. The embryo transfer tube according to claim 18, characterized in that: The needle end of the injection tube is a soft tube.

20. The embryo transfer tube according to claim 18, characterized in that: The one-way tube can be connected to the tube body and keep sealed, and the one-way tube is fixed on the injection tube.

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