A method for increasing inert organic carbon in deep soil layers based on deep-dwelling earthworms

Abstract

The present invention provides a method for increasing the deep-layer inert organic carbon in the soil based on deep-dwelling earthworms, comprising weeding the ground and leveling the land on a plot to obtain a pre-treated plot. Field irrigation is performed on the pre-treated plot to obtain an irrigated plot. Deep-dwelling earthworms are placed on the soil surface of the irrigated plot to increase the total organic carbon content and the inert organic carbon content in the soil by the deep-dwelling earthworms. Analysis of the soil carbon sequestration results showed that when deep-dwelling earthworms were used alone to improve the plot soil, the soil organic carbon reserves increased by 21.0% and the soil inert organic carbon reserves increased by 29.3%. The core of the carbon sequestration of the present invention is to increase the content of soil inert organic carbon in the 40-80 cm soil layer underground. Compared with active organic carbon, inert organic carbon has a longer sequestration time in the soil. In addition, deep soil is not easily disturbed, which further extends the preservation time of organic carbon in the soil. The above method is also significantly environmentally friendly and can achieve effective utilization of waste.

Description

Technical Field

[0001] The present invention relates to the technical field of biological carbon fixation, and in particular to a method for increasing inert organic carbon in deep soil layers based on deep-dwelling earthworms. Background Art

[0002] As an important means of mitigating global climate change, soil carbon sequestration technology is attracting increasing attention for its application and development. However, current soil carbon sequestration strategies have encountered some challenges in practice, primarily in terms of sequestration depth, organic carbon activity, and economic benefits.

[0003] First, regarding carbon sequestration depth, most current soil carbon sequestration technologies focus on the surface soil layer (0-30 cm). While this layer is easy to sequester, it is less effective due to soil disturbance caused by natural and human factors such as tillage and soil erosion. In contrast, deeper soil layers (40-80 cm) exhibit better carbon sequestration due to their stability and low levels of disturbance. Therefore, future soil carbon sequestration technologies should focus more on the carbon sequestration potential of deeper soil layers to improve the stability and durability of carbon sequestration.

[0004] Secondly, considering the activity characteristics of organic carbon, while soil carbon sequestration technologies such as returning organic matter to the field can significantly increase soil organic carbon content, the content of active components in this organic carbon is extremely high. This means that while this active organic carbon can rapidly increase soil carbon reserves in the short term, in the long term, due to its easy decomposition, its carbon sequestration effect is not ideal. Therefore, future soil carbon sequestration technologies should shift towards more stable, inert organic carbon to achieve long-term carbon sequestration.

[0005] In summary, future soil carbon sequestration technologies need to balance and optimize the three aspects of soil depth, organic carbon activity, and economic benefits. By exploring deep soil carbon sequestration, inert organic carbon sequestration, and economically beneficial carbon sequestration models, the important role of soil carbon sequestration in mitigating global climate change can be better played. Summary of the Invention

[0006] To overcome the problems existing in related technologies, the present invention provides a method for increasing inert organic carbon in the deep soil layer using deep-dwelling earthworms. This method can increase the inert organic carbon content in the soil layer 40-80 cm below the surface. Inert organic carbon is an ideal carbon-sequestering material that can effectively increase the soil's carbon storage capacity. Furthermore, deep soil is less susceptible to disturbance, which helps further prolong the retention of organic carbon in the soil.

[0007] Methods for increasing inert organic carbon in the deep soil layer based on deep-dwelling earthworms include:

[0008] Weeding and leveling the land are performed on the plot to obtain a pre-treated plot;

[0009] irrigating the pretreated plot in the field to obtain an irrigated plot;

[0010] Deep-dwelling earthworms are placed on the soil surface of the irrigated plot, and the total organic carbon content and inert organic carbon content of the soil are increased by the deep-dwelling earthworms.

[0011] In a preferred technical solution of the present invention, before releasing deep-dwelling earthworms onto the soil surface of the irrigated land, the method further comprises:

[0012] Dividing the irrigated plot into N rows, where N is greater than or equal to 2;

[0013] Honeysuckle trees were transplanted into the area corresponding to the N rows of the irrigated plot.

[0014] In a preferred technical solution of the present invention, the method of transplanting honeysuckle trees in the area corresponding to the N rows of the irrigated plot includes:

[0015] Set the planting row spacing of honeysuckle trees to 2 meters;

[0016] Set the column spacing of honeysuckle trees in the same row to 2 meters;

[0017] Each honeysuckle tree occupies an area of ​​4 square meters;

[0018] A plurality of honeysuckle trees are planted in the irrigated plot according to the planting row spacing, the column spacing and the land area.

[0019] In a preferred technical solution of the present invention, the step of placing deep-dwelling earthworms on the soil surface of the irrigated land comprises:

[0020] Place earthworms on the soil surface in the release environment with a release density of 500g / m 2 ; Wherein, the seedling placement environment is a seedling sowing temperature less than or equal to 25°C, and the light intensity less than or equal to 5000 lux.

[0021] In a preferred technical solution of the present invention, the field irrigation of the pretreated plot to obtain the irrigated plot comprises:

[0022] The pretreated plot was irrigated and sprayed for 7 consecutive days to maintain 70% of the field capacity to obtain an irrigated plot.

[0023] In a preferred technical solution of the present invention, after increasing the total organic carbon content and the inert organic carbon content in the soil by the deep-dwelling earthworms, the method further comprises:

[0024] Use a soil drill to collect the soil layer 0-80 cm below the ground after the deep-dwelling earthworms are released;

[0025] Layered according to 0-20cm, 20-40cm, 40-60cm and 60-80cm;

[0026] The total organic carbon content and inert organic carbon content in the soil were tested layer by layer.

[0027] In a preferred technical solution of the present invention, after obtaining the irrigated land, the method further comprises:

[0028] A water channel is set up around the irrigated plot as an isolation zone to prevent deep-dwelling earthworms from escaping;

[0029] A sprinkler belt is installed on the irrigated plot, and the sprinkler belt is used for daily watering.

[0030] In a preferred technical solution of the present invention, the ridge height of the water channel is 20 cm, and the single hole flow rate of the sprinkler belt is 2 L / h.

[0031] In a preferred technical solution of the present invention, before releasing deep-dwelling earthworms onto the soil surface of the irrigated land, the method further comprises:

[0032] The electric shock method was used to collect mature earthworms with annular bands; the earthworm trap used in the electric shock method had an input voltage of 12V and an output voltage of 3600V.

[0033] In a preferred technical solution of the present invention, after the deep-dwelling earthworms are released onto the soil surface of the irrigated land, the method further comprises:

[0034] After the deep-dwelling earthworms were released, the plots were watered every 15 days;

[0035] Detecting the field water holding capacity of the soil, and stopping watering if the field water holding capacity of the soil is equal to 100%;

[0036] Fertilizer should be applied every 7-15 days.

[0037] The beneficial effects of the present invention are:

[0038] The method for increasing the deep-layer inert organic carbon in the soil based on deep-dwelling earthworms provided by the present invention includes weeding the ground and leveling the land on the plot to obtain a pre-treated plot. The pre-treated plot is irrigated in the field to obtain an irrigated plot. Deep-dwelling earthworms are placed on the soil surface of the irrigated plot to increase the total organic carbon content and the inert organic carbon content in the soil by the deep-dwelling earthworms. Analysis of the soil carbon sequestration results showed that when deep-dwelling earthworms were used alone to improve the plot soil, the soil organic carbon reserve increased by 21.0% and the soil inert organic carbon reserve increased by 29.3%. The core of the carbon sequestration of the present invention is to increase the content of soil inert organic carbon in the 40-80cm soil layer underground. Compared with active organic carbon, inert organic carbon is more difficult to be eaten and decomposed by soil animals and microorganisms, and its sequestration time in the soil is longer, even up to 2-11 times that of active organic carbon. This characteristic makes inert organic carbon an ideal carbon sequestration material that can effectively improve the carbon storage capacity of the soil. In addition, deep soil is not easily disturbed, further extending the preservation time of organic carbon in the soil. This method significantly extends the sequestration time of organic carbon in soil while also being remarkably environmentally friendly. During implementation, soil inoculated with deep-dwelling earthworms can absorb agricultural waste, such as cow dung, thereby achieving effective waste utilization. This not only helps reduce carbon emissions from agricultural waste and mitigates environmental pollution, but also provides an effective waste disposal method for agricultural production, thereby promoting sustainable agricultural development. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 This is a schematic diagram of the present invention's deep soil carbon sequestration using deep-dwelling earthworms and honeysuckle trees;

[0040] Figure 2 This is a flow chart of the present invention for achieving deep soil carbon sequestration using deep-dwelling earthworms alone;

[0041] Figure 3 This is a flow chart of the present invention for combining deep-dwelling earthworms and honeysuckle trees to achieve deep soil carbon sequestration;

[0042] Figure 4 is the total organic carbon content of soil at different depths of the present invention;

[0043] Figure 5 is the soil inert organic carbon content of soil layers at different depths of the present invention;

[0044] Figure 6 is a regression analysis graph of the soil inert organic carbon content and the soil total organic carbon content of the present invention;

[0045] Figure 7 is a graph showing the experimental results of total soil organic carbon storage in different plots of land according to the present invention;

[0046] Figure 8It is a graph showing the experimental results of soil inert organic carbon storage in different plots of land according to the present invention. DETAILED DESCRIPTION

[0047] The preferred embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. Although preferred embodiments of the present invention are shown in the accompanying drawings, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments described herein. Rather, these embodiments are provided to make the present invention more thorough and complete and to fully convey the scope of the present invention to those skilled in the art.

[0048] Example 1

[0049] like Figure 2 As shown, this embodiment provides a method for increasing inert organic carbon in deep soil layers based on deep-dwelling earthworms, comprising:

[0050] S1: Weeding and leveling the land to obtain a pre-treated land.

[0051] S2: performing field irrigation on the pretreated plot to obtain an irrigated plot.

[0052] S3: releasing deep-dwelling earthworms onto the soil surface of the irrigated plot, thereby increasing the total organic carbon content and inert organic carbon content of the soil through the deep-dwelling earthworms.

[0053] The plots were weeded and leveled to make them smooth. Irrigation was then performed on the pre-treated plots for seven consecutive days, maintaining 70% of field capacity. This yielded irrigated plots. Ground levelness was maintained at no greater than 5 cm. A water channel was installed around the irrigated plots as a barrier to prevent the escape of deep-dwelling earthworms. Sprinkler irrigation systems were installed on the irrigated plots for daily watering. The ridge height of the water channel was 20 cm, and the flow rate per hole of the sprinkler irrigation system was 2 L / h.

[0054] The step of placing deep-dwelling earthworms on the soil surface of the irrigated plot comprises:

[0055] Place earthworms on the soil surface in the release environment with a release density of 500g / m 2 ; Wherein, the seedling placement environment is a seedling sowing temperature less than or equal to 25°C, and the light intensity less than or equal to 5000 lux.

[0056] Preferably, the deep-dwelling earthworm is the Earthworm Lumbricus quinquefolius. Adult earthworms with reproductive rings and body color ranging from brown to purple-gray are used for testing, and old species with black body color are not used. The diet of deep-dwelling earthworms is mainly herbivorous and supplemented by soil feeding. They live in the soil at a depth of 40-80 cm underground and dig vertical burrows to the surface to feed on organic matter at night. In order to achieve long-term carbon fixation, earthworms need to be able to reproduce. The reproductive ring means that the earthworms are sexually mature and can mate and reproduce. Therefore, Earthworms with reproductive rings are used. On the one hand, Earthworms can migrate above-ground organic matter to deep soil, thereby achieving deep soil carbon fixation. On the other hand, Earthworms feed on organic matter and wrap their excrement in soil aggregates to increase soil inert organic carbon.

[0057] Long-term carbon sequestration requires continuous reproduction by earthworms. The reproductive ring signifies that the earthworms are sexually mature and can mate and reproduce. Using Earthworms has several advantages: First, Earthworms can mobilize above-ground organic matter into the deeper soil layers, thereby sequestering carbon in these layers. Second, Earthworms feed on organic matter and encapsulate their excrement in soil aggregates, thereby increasing the inert organic carbon in the soil.

[0058] Before placing deep-dwelling earthworms on the soil surface of the irrigated plot, the method further comprises:

[0059] The electric shock method was used to collect mature earthworms with annular bands; the earthworm trap used in the electric shock method had an input voltage of 12V and an output voltage of 3600V.

[0060] In September of each year, a groundworm trap with an input voltage of 12V and an output voltage of 3600V is used to collect mature earthworms with obvious rings. Some of the earthworms are applied to the soil surface of the irrigated plot, and the remaining earthworms are cleaned and dried to prepare dried earthworm medicinal materials.

[0061] After placing deep-dwelling earthworms on the soil surface of the irrigated plot, the method further comprises:

[0062] After the deep-dwelling earthworms were released, the plots were watered every 15 days;

[0063] Detecting the field water holding capacity of the soil, and stopping watering if the field water holding capacity of the soil is equal to 100%;

[0064] Fertilizer should be applied every 7-15 days.

[0065] The fertilizer in this embodiment is cow dung, and the amount of cow dung placed is 4kg / m 2 / moon.

[0066] After increasing the total organic carbon content and the inert organic carbon content in the soil by the deep-dwelling earthworms, the method further comprises:

[0067] S41: Use a soil auger to collect the soil layer 0-80 cm below the ground after the deep-dwelling earthworms are released.

[0068] S42: Layered according to 0-20cm, 20-40cm, 40-60cm and 60-80cm.

[0069] S43: Detect the total organic carbon content and inert organic carbon content in the soil layer by layer.

[0070] like Figure 4 As shown, for the earthworm plots that only used deep-dwelling earthworms to achieve deep soil carbon fixation, the total soil organic carbon content in the soil layer with a depth of 0-20 cm was 8.3 g / kg. In comparison, the total soil organic carbon content of the blank plots without the application of deep-dwelling earthworms was 4.2 g / kg. The total soil organic carbon content of the earthworm plots was twice that of the blank plots.

[0071] like Figure 5 As shown, for the earthworm plots that used deep-dwelling earthworms alone to achieve deep soil carbon sequestration, the soil inert organic carbon content in the soil layer at a depth of 0-20 cm was 5 g / kg. In comparison, the soil inert organic carbon content in the blank plots without deep-dwelling earthworms was 2.8 g / kg. In this depth range, the soil inert organic carbon content of the earthworm plots was 78.6% higher than that of the blank plots. For the earthworm plots that used deep-dwelling earthworms alone to achieve deep soil carbon sequestration, the soil inert organic carbon content in the soil layer at a depth of 20-40 cm was 5.6 g / kg. In comparison, the soil inert organic carbon content in the blank plots without deep-dwelling earthworms was 3.4 g / kg. In this depth range, the soil inert organic carbon content of the earthworm plots was 64.7% higher than that of the blank plots. For the earthworm plots that used deep-dwelling earthworms alone to achieve deep soil carbon fixation, the soil inert organic carbon content of the soil at a depth of 40-60 cm was 2.5 g / kg. In comparison, the soil inert organic carbon content of the blank plots that did not apply deep-dwelling earthworms was 2.3 g / kg. Within this depth range, the soil inert organic carbon content of the earthworm plots was 8.7% higher than that of the blank plots.

[0072] like Figure 6 As shown in Figure 2, the total organic carbon in the soil increases with the increase of inert organic carbon. The inert organic carbon content in the soil is taken as the independent variable x, and the total organic carbon content in the soil is taken as the dependent variable y. The relationship between y and x is as follows: y = 1.4072x - 0.1035, and the determination coefficient R 2 It is 0.8854. The coefficient of determination indicates how well the model fits the data. The larger the coefficient of determination, the better the simulation fits the data.

[0073] like Figure 7 As shown in Figure 2, the total soil organic carbon storage in the deep-dwelling earthworm plot alone increased by 21.0%. Figure 8 As shown, the soil inert organic carbon storage in the deep-dwelling earthworm plot alone increased by 29.3%.

[0074] The present method of increasing inert organic carbon deep in the soil using deep-dwelling earthworms is not only low-cost and environmentally friendly, but also offers significant economic benefits. The primary purpose of this method is to avoid human or natural disturbances that accelerate the decomposition and mineralization of organic carbon, and to significantly extend the storage time of active organic carbon in the soil, taking into account the difficulty of preserving active organic carbon in the soil.

[0075] The method of increasing inert organic carbon in deep soil layers based on deep-dwelling earthworms in this embodiment has broad application prospects and is environmentally friendly while fixing carbon, thereby providing a new solution for addressing global climate change and promoting sustainable agricultural development.

[0076] The method provided in this embodiment for increasing the deep-layer inert organic carbon in the soil based on deep-dwelling earthworms includes weeding the ground and leveling the land on the plot to obtain a pre-treated plot. The pre-treated plot is irrigated in the field to obtain an irrigated plot. Deep-dwelling earthworms are placed on the soil surface of the irrigated plot to increase the total organic carbon content and the inert organic carbon content in the soil by deep-dwelling earthworms. Analysis of the soil carbon sequestration results showed that when deep-dwelling earthworms were used alone to improve the soil of the plot, the soil organic carbon reserve increased by 21.0% and the soil inert organic carbon reserve increased by 29.3%. The core of the carbon sequestration of the present invention is to increase the content of soil inert organic carbon in the 40-80 cm soil layer underground. Compared with active organic carbon, inert organic carbon is more difficult to be eaten and decomposed by soil animals and microorganisms, and has a longer sequestration time in the soil, which can even reach 2-11 times that of active organic carbon. This characteristic makes inert organic carbon an ideal carbon sequestration material that can effectively improve the carbon storage capacity of the soil. In addition, deep soil is not easily disturbed, further extending the preservation time of organic carbon in the soil. This method significantly extends the sequestration time of organic carbon in soil while also being remarkably environmentally friendly. During implementation, soil inoculated with deep-dwelling earthworms can absorb agricultural waste, such as cow dung, thereby achieving effective waste utilization. This not only helps reduce carbon emissions from agricultural waste and mitigates environmental pollution, but also provides an effective waste disposal method for agricultural production, thereby promoting sustainable agricultural development.

[0077] Example 2

[0078] like Figure 3 As shown, this embodiment provides a method for increasing inert organic carbon in deep soil layers based on deep-dwelling earthworms, comprising:

[0079] S1: Weeding and leveling the land to obtain a pre-treated land.

[0080] S2: performing field irrigation on the pretreated plot to obtain an irrigated plot.

[0081] S3: Divide the irrigated plot into N rows, where N≥2.

[0082] S4: Transplanting honeysuckle trees in the area corresponding to the N rows of the irrigated plot.

[0083] S5: releasing deep-dwelling earthworms onto the soil surface of the irrigated plot, thereby increasing the total organic carbon content and inert organic carbon content of the soil through the deep-dwelling earthworms.

[0084] The step of transplanting honeysuckle trees in the area corresponding to the N rows of the irrigated plot comprises:

[0085] S41: Set the planting row spacing of honeysuckle trees to 2 meters.

[0086] S42: Set the column spacing of honeysuckle trees in the same row to 2 meters.

[0087] S43: Each honeysuckle tree occupies an area of ​​4 square meters.

[0088] S44: Planting a plurality of honeysuckle trees in the irrigated plot according to the planting row spacing, the column spacing and the occupied area.

[0089] like Figure 1 As shown, the spacing between rows and columns of honeysuckle trees is 2 meters. The trees are taller than 1 meter, provide shade greater than 0.5 square meters, and are planted at a density of one tree per 4 square meters. Honeysuckle flowers are hand-harvested annually in May, dried, and used as tea. Mature, annular earthworms (E. quinquefasciatus) are collected annually in September. Some are applied to the soil surface, while others are dried for bowel cleansing to create dried earthworm medicinal materials.

[0090] The step of placing deep-dwelling earthworms on the soil surface of the irrigated plot comprises:

[0091] Place earthworms on the soil surface in the release environment with a release density of 500g / m 2 ; Wherein, the seedling placement environment is a seedling sowing temperature less than or equal to 25°C, and the light intensity less than or equal to 5000 lux.

[0092] After increasing the total organic carbon content and the inert organic carbon content in the soil by the deep-dwelling earthworms, the method further comprises:

[0093] S61: Use a soil drill to collect the soil layer 0-80 cm below the ground after the deep-dwelling earthworms are released.

[0094] S62: Layered according to 0-20cm, 20-40cm, 40-60cm and 60-80cm.

[0095] S63: Detect the total organic carbon content and inert organic carbon content in the soil layer by layer.

[0096] like Figure 4 As shown in the results, by cultivating deep-dwelling earthworms and planting honeysuckle, the total organic carbon content in the deep soil layer of 40-80 cm increased by 12.8-14.6%, and the organic carbon content in all soil layers increased by 9.31%-105.5%. Figure 5 As shown in the figure, by cultivating deep-dwelling earthworms and planting honeysuckle, the inert organic carbon content in the deep soil layer of 40-80 cm increased by 13.8-22.1%, and the organic carbon content in all soil layers increased by 13.8-94.1%. Figure 6 As shown in Figure 2, total organic carbon in soil increases with the increase of inert organic carbon. Figure 7 As shown in Figure 2, cultivating deep-dwelling earthworms and planting honeysuckle trees increased soil organic carbon storage by 36.2%. Figure 8 As shown, cultivating deep-dwelling earthworms and planting honeysuckle trees increased the soil inert organic carbon storage by 31.2%.

[0097] In this embodiment, deep-dwelling earthworms are added to the soil surface on the basis of planting honeysuckle trees. The litter and root secretions of the honeysuckle trees increase the input of exogenous organic matter, thereby improving the content of soil organic carbon as a whole. Figure 5 When the soil depth is 0-20cm and 20-40cm, the soil inert organic carbon content of the plots improved with earthworms alone is higher than that of the plots improved with a combination of earthworms and honeysuckle. The reason is that the soil layers corresponding to the above two soil depth ranges are the main distribution areas of the honeysuckle tree roots, and the rich rhizosphere microorganisms have a significant activation effect on soil organic carbon.

[0098] It should be noted that, in this document, the terms "comprises," "includes," or any other variations thereof are intended to encompass non-exclusive inclusion, such that a process, apparatus, article, or method comprising a series of elements includes not only those elements but also other elements not explicitly listed, or elements inherent to such process, apparatus, article, or method. In the absence of further limitations, an element defined by the phrase "comprising a ..." does not exclude the presence of other identical elements in the process, apparatus, article, or method comprising the element.

[0099] The above description is only a preferred embodiment of the present application and does not limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the contents of the present application specification and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A method for increasing inert organic carbon in deep soil layers based on deep-dwelling earthworms, characterized in that: include: Weeding and leveling the land are performed on the plot to obtain a pre-treated plot; irrigating the pretreated plot in the field to obtain an irrigated plot; placing deep-dwelling earthworms on the soil surface of the irrigated plot, thereby increasing the total organic carbon content and the inert organic carbon content of the soil through the deep-dwelling earthworms; Before placing deep-dwelling earthworms on the soil surface of the irrigated plot, the method further comprises: Dividing the irrigated plot into N rows, where N is greater than or equal to 2; Transplanting honeysuckle trees in the area corresponding to the N rows of the irrigated plot; The step of transplanting honeysuckle trees in the area corresponding to the N rows of the irrigated plot comprises: Set the planting row spacing of honeysuckle trees to 2 meters; Set the column spacing of honeysuckle trees in the same row to 2 meters; Each honeysuckle tree occupies an area of ​​4 square meters; Planting a plurality of honeysuckle trees in the irrigated plot according to the planting row spacing, the column spacing and the land area; After increasing the total organic carbon content and the inert organic carbon content in the soil by the deep-dwelling earthworms, the method further comprises: Use a soil drill to collect the soil layer 0-80 cm below the ground after the deep-dwelling earthworms are released; Layered according to 0-20cm, 20-40cm, 40-60cm and 60-80cm; The total organic carbon content and inert organic carbon content in the soil were tested layer by layer.

2. The method for increasing deep soil inert organic carbon based on deep-dwelling earthworms according to claim 1, characterized in that: The step of placing deep-dwelling earthworms on the soil surface of the irrigated plot comprises: Place earthworms on the soil surface in the release environment with a release density of 500g / m 2 ; Wherein, the seedling placement environment is a seedling sowing temperature less than or equal to 25°C, and the light intensity less than or equal to 5000 lux.

3. The method for increasing inert organic carbon in deep soil layers based on deep-dwelling earthworms according to claim 1, characterized in that: The method of performing field irrigation on the pretreated plot to obtain an irrigated plot comprises: The pretreated plot was irrigated and sprayed for 7 consecutive days to maintain 70% of the field capacity to obtain an irrigated plot.

4. The method for increasing inert organic carbon in deep soil layers based on deep-dwelling earthworms according to claim 1, characterized in that: After obtaining the irrigated land, the method further includes: A water channel is set up around the irrigated plot as an isolation zone to prevent deep-dwelling earthworms from escaping; A sprinkler belt is installed on the irrigated plot, and the sprinkler belt is used for daily watering.

5. The method for increasing inert organic carbon in deep soil layers based on deep-dwelling earthworms according to claim 4, characterized in that: The ridge height of the water channel is 20 cm, and the single hole flow rate of the sprinkler belt is 2 L / h.

6. The method for increasing inert organic carbon in deep soil layers based on deep-dwelling earthworms according to claim 1, characterized in that: Before placing deep-dwelling earthworms on the soil surface of the irrigated plot, the method further comprises: The electric shock method was used to collect mature earthworms with annular bands; the earthworm trap used in the electric shock method had an input voltage of 12V and an output voltage of 3600V.

7. The method for increasing inert organic carbon in deep soil layers based on deep-dwelling earthworms according to claim 1, characterized in that: After placing deep-dwelling earthworms on the soil surface of the irrigated plot, the method further comprises: After the deep-dwelling earthworms were released, the plots were watered every 15 days; Detecting the field water holding capacity of the soil, and stopping watering if the field water holding capacity of the soil is equal to 100%; Fertilizer should be applied every 7-15 days.

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