A solubilized emulsion based on mogroside, and its preparation method and application

Abstract

The present invention relates to the field of food additive technology, and discloses a solubilized emulsion based on mogroside, a preparation method thereof, and an application thereof. The raw materials include a composite amphiphilic emulsifier, a medium-chain triglyceride oily liquid, an amphiphilic solubilizing composite lipid, and mogroside. The present invention employs a composite lipid having the functions of solubilizing and adjusting density, as well as an amphoteric solubilizing emulsifier such as a monoglyceride substance, to effectively achieve the stable dissolution of mogroside in a medium-chain triglyceride oily core liquid. The preparation method of the solubilized emulsion based on mogroside involved in the present invention is characterized by a simple process, low cost, and safety. The method does not require the use of organic solvents or special preparation equipment and processes, and significantly improves the solubility of mogroside without changing the composition of the core liquid of the popping bead candy.

Description

Technical Field

[0001] The present invention relates to the technical field of food additives, in particular to a mogroside solubilized emulsion and application thereof in food additives. Background Art

[0002] Mogroside, also known as mogroside or monk fruit sweet, is a white or pale yellow powder produced through a process involving the selection, extraction, and filtration of monk fruit. Its melting point is between 197 and 201 degrees Celsius, at which it decomposes. It is naturally sweet, 300 times sweeter than sucrose. Compared to other sweeteners, mogroside offers unparalleled advantages, including zero calories, tooth decay prevention, and no toxic side effects. It is found in high concentrations in plants and is highly water-soluble. Products with a purity exceeding 98% are currently used as food additives in a wide range of applications, including pharmaceuticals, food, beverages, and health supplements. Due to its excellent water solubility, mogroside is increasingly being used as a natural ingredient in a wide range of industries.

[0003] As an emerging functional soft drink, popping beads have gradually won the favor of consumers. Popping beads are mainly composed of inclusions (also known as core liquid) and shell materials. When the popping beads rupture, the core liquid can release liquid components, achieving the release effect of beneficial ingredients. The choice of shell material of the popping beads has a decisive influence on the mechanical properties and permeability of the product. Calcium alginate salt is a shell material widely used in the pharmaceutical, food and beverage industries. The popping beads products produced by the drip method with calcium ion solution as the coagulation bath have been industrialized and widely promoted in the market.

[0004] In order to enhance the nutritional value, flavor and efficacy of popping beads, specific ingredients can be added to the popping bead core liquid to prepare popping bead products with different flavors and efficacy. In addition to drop-made products based on calcium alginate salt, the popping bead candies currently on the market also include products made with gelatin, carrageenan, agar, pectin, modified starch and other wall materials. The main components of these wall materials are hydrophilic colloids. Due to their hydrophilic properties, this type of wall material is only suitable for encapsulating oily core liquids and their oil-soluble flavoring ingredients. However, many edible flavors are water-soluble or alcohol-soluble, making them difficult to incorporate into existing popping bead products, which significantly limits their scope of application. Summary of the Invention

[0005] In view of this, the present invention proposes a mogroside solubilized emulsion and its application in food additives, aiming to solve the problems existing in the prior art, provide a mogroside solubilized emulsion that can effectively solubilize water-soluble or alcohol-soluble edible flavors, and apply it to food additives to expand the flavor and efficacy of popping bead products.

[0006] The present invention provides a method for preparing a solubilized emulsion based on mogroside, the preparation method comprising:

[0007] The composite amphiphilic emulsifier and the medium-chain triglyceride oily liquid are mixed, heated in a water bath, and stirred to obtain a binary mixed system;

[0008] Adding amphiphilic solubilizing complex lipid to the binary mixed system and stirring evenly to obtain a ternary mixed system;

[0009] Adding mogroside to the ternary mixed system, heating in a water bath and stirring, to obtain the mogroside-based solubilized emulsion.

[0010] Preferably, the weight ratio of the composite amphiphilic emulsifier, medium-chain triglyceride oily liquid, amphiphilic solubilizing complex lipid and mogroside is 0.5-5:0.3-3:90-98:0.1-2.

[0011] Preferably, the composite amphiphilic emulsifier is composed of a composite lipid substance and an emulsifier;

[0012] The weight ratio of the complex lipid substance to the emulsifier is 0.3-3:0.5-5;

[0013] The complex lipid substance is a monoglyceride compound.

[0014] Preferably, the emulsifier is mono- and diglycerol fatty acid ester, sucrose fatty acid ester, propylene glycol fatty acid ester, sorbitol fatty acid ester, xylitol monostearate or phospholipid.

[0015] Preferably, the amphiphilic solubilizing complex lipid is monoglyceride.

[0016] Preferably, when the composite amphiphilic emulsifier and the medium-chain triglyceride oily liquid are mixed and heated in a water bath with stirring, the heating temperature is 20-80°C.

[0017] Preferably, when mogroside is added to the ternary mixed system and heated in a water bath with stirring, the heating temperature is 20-50°C.

[0018] The present invention also provides a solubilized emulsion based on mogroside, which is prepared according to the above-mentioned method for preparing the solubilized emulsion based on mogroside.

[0019] The present invention also proposes an application of the mogroside-based solubilized emulsion in food additives.

[0020] The present invention also provides a food additive, the main component of which is the above-mentioned mogroside-based solubilized emulsion.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] By incorporating amphoteric solubilizing emulsifiers such as complex lipids with solubilizing and density regulating functions and monoglycerides with emulsifying effects, the stable solubilization of mogroside in the medium-chain triglyceride oily core liquid was successfully achieved; by adjusting the mogroside solubilized emulsion, while introducing sweeteners, the addition of complex lipids further enhanced the nutritional value and efficacy of this type of core liquid bursting bead products.

[0023] The method for preparing a solubilized emulsion based on mogroside provided by the present invention is simple, low-cost, safe, and harmless. It does not require the use of organic solvents or special preparation equipment and processes, and significantly improves the solubility of mogroside without changing the composition of the popping bead candy core liquid. The solubilized emulsion obtained by this preparation method expands the application of mogroside as a food additive and provides new possibilities for sweetening oily core liquids. DETAILED DESCRIPTION

[0024] The exemplary embodiments of the present disclosure will be described in more detail below. Although exemplary embodiments of the present disclosure are shown below, it should be understood that the present disclosure can be implemented in various forms and should not be limited by the embodiments set forth herein. On the contrary, these embodiments are provided to enable a more thorough understanding of the present disclosure and to fully convey the scope of the present disclosure to those skilled in the art. It should be noted that, unless there is a conflict, the embodiments of the present disclosure and the features described in the embodiments can be combined with each other. The present invention will be described in detail below in conjunction with the embodiments.

[0025] The invention relates to a solubilized emulsion based on mogroside, which comprises by weight 0.3-3 weight percent (wt%) of a complex lipid and 0.5-5 weight percent (wt%) of amphoteric solubilizing emulsifiers such as monoglycerides, as well as 0.1-2 weight percent (wt%) of mogroside and 90-98 weight percent (wt%) of a medium-chain triglyceride oily liquid.

[0026] In the practice of the present invention, when the content of amphoteric solubilizing emulsifiers such as complex lipids and monoglycerides is within the above range, if the content of the complex lipid is less than 0.3 weight percent (wt%), the solubilization effect on mogroside is poor, resulting in poor fluidity of the emulsified mogroside; if it is greater than 3 weight percent (wt%), the solubilization effect on mogroside is also poor, the density of the emulsified mogroside is significantly different from that of the system, and it is easy to separate after standing, and the solubilization effect and stability of the mogroside solubilized emulsion cannot be ensured. For monoglyceride amphoteric solubilizing emulsifiers, if its content is less than 0.5 weight percent (wt%), the emulsification effect is poor, the solubilization effect on mogroside is poor, and undissolved mogroside precipitation is easily generated; if it is greater than 5 weight percent (wt%), it will lead to over-emulsification, making the system appear paste-like and lose fluidity. Therefore, the content of the amphoteric solubilizing emulsifier is preferably 0.3-3 weight percent (wt%) of complex lipids and 0.5-5 weight percent (wt%) of monoglycerides.

[0027] In the practice of the present invention, when the amounts of the compound lipid and amphoteric solubilizing emulsifiers, such as monoglycerides, are within the above ranges, their amounts need to be adjusted according to the compounding ratio. When the content ratio of the compound lipid to the monoglyceride is less than 0.2 or between 0.25 and 0.3, it will lead to over-emulsification and poor system fluidity. When the content ratio of the compound lipid to the monoglyceride is greater than 0.3, the amount of the compound lipid is too large, the density difference of the system is large, and stratification after emulsification occurs, and the emulsifier cannot completely disperse the mogroside. Therefore, the content ratio of the compound lipid to the monoglyceride is preferably between 0.2 and 0.25.

[0028] The technical solution of the present invention is described in detail below through specific embodiments.

[0029] In the following examples, the mogroside (Huacheng Biological, food grade) used was purchased from Hunan Huacheng Biological Resources Co., Ltd.; the amphoteric solubilizing emulsifiers such as the complex lipids and monoglycerides used were all products sold on the market, such as Midaner mono- and diglycerol fatty acid esters (food grade) sold by Zhengzhou Dahe Food Technology Co., Ltd., sugar cabinet sucrose fatty acid esters (food grade) sold by Guangxi Fusai Food Additives Co., Ltd., propylene glycol fatty acid esters (food grade) sold by Henan Honest Food Co., Ltd., Runhua sorbitol fatty acid esters sold by Guangzhou Junxin Chemical Technology Co., Ltd., xylitol anhydride monostearate (food grade) sold by Henan Weidumei Biotechnology Co., Ltd., and phospholipids sold by Hebei Meiye Siwei Biotechnology Co., Ltd.

[0030] Example 1

[0031] A solubilized emulsion based on mogroside and its preparation method involves the following components in the following mass ratios: 0.50 wt% mogroside, 1.00 wt% amphiphilic solubilizing complex lipid, 4.00 wt% monoglyceride amphiphilic emulsifier (mono- and diglyceride fatty acid esters), and 94.50 wt% medium-chain triglyceride oily liquid. The components must be mixed in a specific order. The preparation steps are detailed below:

[0032] First, 4.00 wt% of monoglyceride amphiphilic emulsifier (mono- and diglyceride fatty acid esters) was mixed with 94.50 wt% of medium-chain triglyceride oily liquid and stirred in a 50°C water bath to form a transparent and uniform binary mixed system;

[0033] Next, 1.00 wt% of an amphiphilic solubilizing complex lipid was added to the clear and uniform binary mixture under heating and stirring in a water bath, and stirred thoroughly to obtain a brownish-yellow, transparent and uniform ternary mixture.

[0034] Then, after the ternary system is uniform, 0.50 wt% of mogroside is added, and the mixture is heated and stirred in a water bath until it is completely dissolved, thereby obtaining a uniform solubilized emulsion (no precipitation occurs after standing in a water bath or at room temperature);

[0035] Finally, the prepared solubilized emulsion was subjected to ultrasonic treatment to achieve a more uniform dispersion of the system, and no obvious precipitation was observed after being placed for a long time.

[0036] Example 2

[0037] A mogroside-based solubilized emulsion and its preparation method involve the following components in the following mass ratios: 0.50wt% mogroside, 0.99wt% amphiphilic solubilizing complex lipid, 4.28wt% composite amphiphilic emulsifier (mono- and diglycerol fatty acid esters to sucrose fatty acid esters in a 2:1 ratio), and 94.23wt% medium-chain triglyceride oily liquid. The components must be mixed in a specific order. The specific preparation steps are as follows:

[0038] First, 4.28 wt% of a composite amphiphilic emulsifier (mono- and diglycerol fatty acid esters to sucrose fatty acid esters in a ratio of 2:1) was mixed with 94.23 wt% of a medium-chain triglyceride oily liquid and stirred in a 60°C water bath to obtain a transparent and uniform binary mixed system.

[0039] Next, the monoglyceride and medium-chain triglyceride oily liquid were mixed in a water bath with heating and stirring until transparent and uniform, and then 0.99 wt% of an amphiphilic solubilizing complex lipid was added and stirred thoroughly to form a brownish-yellow, transparent and uniform ternary mixed system.

[0040] Then, 0.50 wt% of mogroside was added to the ternary system, and the mixture was heated and stirred in a water bath until it was completely dissolved, thereby obtaining a solubilized emulsion with uniform properties (no precipitation was observed after standing in a water bath or at room temperature);

[0041] Finally, the prepared solubilized emulsion was subjected to ultrasonic treatment to achieve a more uniform dispersion of the system, and no obvious precipitation was observed after being placed for a long time.

[0042] Example 3

[0043] A solubilized emulsion based on mogroside and a preparation method thereof comprises the following components in the following mass ratios: 0.50 wt% mogroside, 1.00 wt% amphiphilic solubilizing complex lipid, 5.00 wt% monoglyceride amphiphilic emulsifier (propylene glycol fatty acid ester), and 93.50 wt% medium-chain triglyceride oily liquid. When mixing the components, the mixing order must be strictly followed. The specific preparation steps are as follows:

[0044] First, 5.00 wt% of monoglyceride amphiphilic emulsifier (propylene glycol fatty acid ester) was mixed with 94.50 wt% of medium-chain triglyceride oily liquid and stirred in a 50°C water bath to form a transparent and uniform binary mixed system;

[0045] Next, the mixture of monoglyceride and medium-chain triglyceride oily liquid was clarified and uniformly mixed under heating and stirring in a water bath, and then 1.00 wt% of an amphiphilic solubilizing complex lipid was added and stirred thoroughly to obtain a brownish-yellow, transparent, and uniform ternary mixed system.

[0046] Then, after the ternary system is uniform, 0.50 wt% of mogroside is added, and the mixture is heated and stirred in a water bath until it is completely dissolved, thereby obtaining a solubilized emulsion with uniform properties (no precipitation occurs after standing in a water bath or at room temperature);

[0047] Finally, the prepared solubilized emulsion was subjected to ultrasonic treatment to make the system more uniformly dispersed, and no obvious precipitation was observed after being placed for a long time.

[0048] Example 4

[0049] A mogroside-based solubilized emulsion and its preparation method involve the following components in the following mass ratios: 0.52% mogroside, 1.01% amphiphilic solubilizing complex lipid, 4.40% composite amphiphilic emulsifier (mono- and diglycerol fatty acid esters to sorbitol fatty acid esters in a 2:1 ratio), and 94.07% medium-chain triglyceride oily liquid. The components must be mixed in a specific order, and the preparation steps are detailed below:

[0050] First, 4.40% of a composite amphiphilic emulsifier (mono- and diglycerol fatty acid esters to sorbitol fatty acid esters in a ratio of 2:1) was mixed with 94.07% of a medium-chain triglyceride oily liquid and stirred in a 60°C water bath to form a transparent and uniform binary mixed system.

[0051] Next, the monoglyceride and medium-chain triglyceride oily liquids were mixed until transparent and uniform, and then 1.01% of an amphiphilic solubilizing complex lipid was added thereto under heating and stirring in a water bath. After thorough stirring, a brownish-yellow, transparent, and uniform ternary mixed system was obtained.

[0052] Then, 0.52% of mogroside was added to the ternary system, and the mixture was heated in a water bath and stirred until it was completely dissolved, thereby obtaining a solubilized emulsion (precipitation may occur after standing in a water bath or at room temperature for a long time);

[0053] Finally, the prepared solubilized emulsion is subjected to ultrasonic treatment to disperse the system. After standing for a long time, the system will separate into layers to form an upper brown-yellow transparent liquid phase and a lower emulsion phase.

[0054] Example 5

[0055] A mogroside-based solubilized emulsion and its preparation method comprises the following components in the following mass ratios: 0.58% mogroside, 3.22% amphiphilic solubilizing complex lipid, 4.07% monoglyceride amphiphilic emulsifier (xylitol monostearate), and 92.13% medium-chain triglyceride oily liquid. When mixing the components, the mixing order must be strictly followed. The specific preparation steps are as follows:

[0056] First, 4.07% of a monoglyceride amphiphilic emulsifier (xylitol monostearate) was mixed with 92.13% of a medium-chain triglyceride oily liquid and stirred in a 60°C water bath until a transparent and uniform binary mixture system was formed.

[0057] Next, the mixture of monoglyceride and medium-chain triglyceride oily liquid was clarified and uniformly mixed under heating and stirring in a water bath, and then 3.22% of amphiphilic solubilizing complex lipid was added thereto. After thorough stirring, a brownish-yellow, transparent and uniform ternary mixed system was obtained.

[0058] Then, after the ternary system is uniform, 0.58% of mogroside is added, and heating and stirring in a water bath are continued until it is completely dissolved, thereby preparing a solubilized emulsion (precipitation may occur after standing in a water bath or at room temperature for a long time);

[0059] Finally, the prepared solubilized emulsion is subjected to ultrasonic treatment to disperse the system. After standing for a long time, stratification may occur, with the upper layer being a brownish-yellow transparent liquid phase and the lower layer being an emulsion.

[0060] Example 6

[0061] A mogroside-based solubilized emulsion and its preparation method involve the following components in the following mass ratios: 0.50 wt% mogroside, 1.11 wt% amphiphilic solubilizing complex lipid, 3.71 wt% composite amphiphilic emulsifier (mono- and diglycerol fatty acid esters to sucrose fatty acid esters in a 2:1 ratio), and 94.68 wt% medium-chain triglyceride oily liquid. The components must be mixed in a specific order, and the preparation steps are detailed as follows:

[0062] First, 3.71 wt% of a composite amphiphilic emulsifier (mono- and diglycerol fatty acid esters to sucrose fatty acid esters in a ratio of 2:1) was mixed with 94.68 wt% of a medium-chain triglyceride oily liquid and stirred in a 50°C water bath to form a transparent and uniform binary mixed system.

[0063] Next, the monoglyceride and medium-chain triglyceride oily liquid were mixed in a water bath under stirring and heating until transparent and uniform, and then 1.11 wt% of an amphiphilic solubilizing complex lipid was added and stirred thoroughly to form a brownish-yellow, transparent and uniform ternary mixed system.

[0064] Then, 0.50 wt% of mogroside was added to the ternary system, and the mixture was heated in a water bath and stirred until it was completely dissolved, thereby obtaining a solubilized emulsion (precipitation may occur after standing in a water bath or at room temperature for a long time);

[0065] Finally, the prepared solubilized emulsion is subjected to ultrasonic treatment to disperse the system. After standing, stratification may occur, with the upper layer being a brownish-yellow transparent liquid phase and the lower layer being an emulsion.

[0066] Example 7

[0067] A mogroside-based solubilized emulsion and its preparation method comprises the following components in the following mass ratios: 0.52 wt% mogroside, 1.14 wt% amphiphilic solubilizing complex lipid, 4.53 wt% monoglyceride amphiphilic emulsifier (phospholipid), and 93.81 wt% medium-chain triglyceride oily liquid. When mixing the components, attention must be paid to the mixing order. The specific preparation steps are as follows:

[0068] First, 4.53 wt% of monoglyceride amphiphilic emulsifier (phospholipid) was mixed with 93.81 wt% of medium-chain triglyceride oily liquid and stirred in a water bath at 60°C to obtain a clear and uniform binary mixed system;

[0069] Next, 1.14 wt% of an amphiphilic solubilizing complex lipid was added to the clear and uniform mixture obtained by mixing the monoglyceride and medium-chain triglyceride oily liquid, and the mixture was thoroughly mixed under heating and stirring in a water bath, thereby obtaining a brownish-yellow, clear, and uniform ternary mixed system.

[0070] Then, after the ternary system was homogenized, 0.52 wt% of mogroside was added, and the mixture was heated in a water bath and stirred until completely dissolved to prepare a solubilized emulsion (no precipitation was observed after standing in a water bath or at room temperature, but the fluidity was poor);

[0071] Finally, the prepared solubilized emulsion was subjected to ultrasonic treatment to achieve a more uniform dispersion of the system. No obvious precipitation was observed after long-term storage, although its fluidity was still poor.

[0072] Example 8

[0073] A mogroside-based solubilized emulsion and its preparation method involve the following components in the following mass ratios: 0.50 wt% mogroside, 1.00 wt% amphiphilic solubilizing complex lipid, 3.00 wt% monoglyceride amphiphilic emulsifier (sucrose fatty acid ester), and 95.50 wt% medium-chain triglyceride oily liquid. The components must be mixed in a specific order. The specific preparation steps are as follows:

[0074] First, a component containing 3.00 wt% of a monoglyceride amphiphilic emulsifier (sucrose fatty acid ester) and a component containing 95.50 wt% of a medium-chain triglyceride oily liquid were mixed and stirred in a 50°C water bath to obtain a clear and uniform binary mixed system;

[0075] Next, after the binary mixture is clear and uniform, a component containing 1.00 wt% of an amphiphilic solubilizing complex lipid is added thereto while continuing to heat and stir in a water bath, and after thorough stirring, a brownish-yellow, clear, and uniform ternary mixture is obtained;

[0076] Then, after the ternary system is uniform, a component containing 0.50 wt% of mogroside is added, and the mixture is heated in a water bath and stirred until completely dissolved, thereby preparing a solubilized emulsion (if the emulsification effect is not good, the mogroside may be separated after standing in a water bath or at room temperature);

[0077] Finally, the prepared solubilized emulsion is subjected to ultrasonic treatment to disperse the system, and stratification may occur after standing for a long time.

[0078] Example 9

[0079] A solubilized emulsion using mogroside as a sweetener and a preparation method thereof, comprising the following components in the following mass ratios: 0.58% mogroside, 4.07% monoglyceride amphiphilic emulsifier (propylene glycol fatty acid ester), and 95.35% medium-chain triglyceride oily liquid. The components must be mixed in a specific order. The specific preparation steps are as follows:

[0080] First, 4.07% of monoglyceride amphiphilic emulsifier (propylene glycol fatty acid ester) and 95.35% of medium-chain triglyceride oil liquid were mixed according to the ratio and stirred in a 60°C water bath until a transparent and uniform binary mixed system was formed;

[0081] Next, 0.58% of mogroside was added to the binary system, and the mixture was heated and stirred in a water bath until the mogroside was completely dissolved. At this point, the resulting solubilized emulsion was in a paste-like state and lacked fluidity.

[0082] Finally, the prepared solubilized emulsion is subjected to ultrasonic treatment, and the treated product remains in a paste-like state and has no fluidity.

[0083] Example 10

[0084] A mogroside-based solubilized emulsion and its preparation method involve the following components in the following mass ratios: 0.10 wt% mogroside, 2.35 wt% amphiphilic solubilizing complex lipid, and 97.55 wt% medium-chain triglyceride oil. The components must be mixed in a strict order. The specific preparation steps are as follows:

[0085] First, 2.35 wt% of an amphiphilic solubilizing complex lipid and 97.55 wt% of a medium-chain triglyceride oily liquid were mixed according to a specific ratio and stirred in a 50°C water bath until a transparent and uniform binary mixture was formed.

[0086] Next, 0.10 wt % of mogroside was added to the binary system, and the mixture was heated and stirred in a water bath until completely dissolved, thereby preparing a uniform mogroside sweetener solution (no precipitation was observed after standing in a water bath or at room temperature);

[0087] Finally, the prepared solubilized emulsion was subjected to ultrasonic treatment to ensure that the system was more evenly dispersed, and no obvious precipitation was observed after being placed for a long time.

[0088] Example 11

[0089] A mogroside-based solubilized emulsion and its preparation method involve the following components in the following mass ratios: 1.94% mogroside, 3.97% amphiphilic solubilizing complex lipid, 15.98% monoglyceride amphiphilic emulsifier (phospholipid), and 78.11% medium-chain triglyceride oily liquid. The components must be mixed in a specific order. The specific preparation steps are as follows:

[0090] First, a component containing 15.98% monoglyceride amphiphilic emulsifier (phospholipid) and 78.11% medium-chain triglyceride oily liquid were mixed and stirred in a 60°C water bath to form a transparent and uniform binary mixed system;

[0091] Next, under the condition of heating and stirring in a water bath, a clear and uniform oily mixture of monoglycerides and medium-chain triglycerides was added with 3.97% of an amphiphilic solubilizing complex lipid, and after thorough stirring, a brownish-yellow, transparent and uniform ternary mixed system was obtained;

[0092] Then, 1.94% mogroside was added to the ternary system, and the mixture was heated and stirred in a water bath until it was completely dissolved, thereby obtaining a uniform solubilized emulsion (no precipitation occurred after standing in a water bath or at room temperature);

[0093] Finally, the prepared solubilized emulsion was subjected to ultrasonic treatment to achieve a more uniform dispersion of the system, and no obvious precipitation was observed after being placed for a long time.

[0094] Example 12

[0095] A mogroside-based solubilized emulsion and its preparation method involve the following components in the following mass ratios: 0.51% mogroside, 1.02% amphiphilic solubilizing complex lipid, 4.11% composite amphiphilic emulsifier (mono- and diglycerol fatty acid esters to xylitol monostearate in a 2:1 ratio), and 94.36% medium-chain triglyceride oily liquid. The components must be mixed in a specific order, and the preparation steps are detailed below:

[0096] First, 4.11% of a composite amphiphilic emulsifier (mono- and diglycerol fatty acid esters and xylitol monostearate in a ratio of 2:1) was mixed with 94.36% of a medium-chain triglyceride oily liquid and stirred in a 60°C water bath to form a transparent and uniform binary mixed system.

[0097] Next, the monoglyceride and medium-chain triglyceride oily liquid were mixed in a water bath under heating and stirring until transparent and uniform, and then 1.02% of an amphiphilic solubilizing complex lipid was added and stirred thoroughly to form a brownish-yellow, transparent and uniform ternary mixed system.

[0098] Then, 0.51% of mogroside was added to the ternary system, and the mixture was heated and stirred in a water bath until it was completely dissolved, thereby obtaining a solubilized emulsion (precipitation may occur after standing in a water bath or at room temperature for a long time);

[0099] Finally, the prepared solubilized emulsion is subjected to ultrasonic treatment to disperse the system. After standing for a long time, the system will separate into layers to form an upper brown-yellow transparent liquid phase and a lower emulsion phase.

[0100] Test Example 1

[0101] The solubilized emulsions of mogroside prepared in Examples 1-4 and 11-12 exhibited uniform properties and good fluidity, with no stratification or precipitation even after prolonged storage. Examples 5-9 demonstrated comparative cases where the proportions of single or combined emulsifiers were exceeded. Example 10, however, was a comparative example in which a large amount of compound lipid was used without an emulsifier, and the dissolution of the compound lipid was still satisfactory. Specific results and comparative examples are detailed in Table 1 below.

[0102] Table 1

[0103]

[0104]

[0105] The present invention utilizes the physical properties of a medium-chain triglyceride (MCT) oily liquid, combined with the properties of mogroside, to enhance its solubility in the MCT oily liquid by adding an amphiphilic substance to promote dissolution. However, due to the high polarity of mogroside, it is incompatible with the non-polar MCT oily liquid. To address this issue, the present invention further introduces single or combined amphiphilic emulsifiers, in addition to solubilization and solubilization-aiding, to enhance the dissolution effect and prepare an emulsion. This method ensures that incompletely dissolved mogroside is evenly dispersed in the mixed liquid, ultimately forming a stable mogroside-solubilized emulsion and preventing the precipitation of mogroside. The resulting mogroside-solubilized emulsion, obtained by water bath or ultrasonic treatment, is evenly dispersed, has consistent properties, and exhibits no significant changes after prolonged standing.

[0106] In summary, the inventive principle and mechanism of the present invention is a mogroside-solubilized emulsion, the mass content of which includes 0.3-3 weight percent of a complex lipid and 0.5-5 weight percent of an amphoteric solubilizing emulsifier such as a monoglyceride substance, as well as 0.1-2 weight percent of mogroside and 90-98 weight percent of a medium-chain triglyceride oily liquid. In the practice of the present invention, when the contents of the complex lipid and the amphoteric solubilizing emulsifier such as a monoglyceride substance are within the above-mentioned range, if the content of the complex lipid is less than 0.3 weight percent, the solubilization effect on mogroside is poor, resulting in poor fluidity of the emulsified mogroside; if the content of the complex lipid is greater than 3 weight percent, the solubilization effect on mogroside is also poor, the density of the emulsified mogroside differs significantly from that of the system, and the emulsified mogroside is easily separated after standing, and the solubilization effect and stability of the mogroside-solubilized emulsion cannot be ensured. For monoglyceride-based amphoteric solubilizing emulsifiers, if their content is less than 0.5 weight percent, the emulsification effect is poor, the solubilization effect on mogroside is poor, and undissolved mogroside precipitation is easily generated. If it is greater than 5 weight percent, it will lead to over-emulsification, making the system appear paste-like and lose fluidity. Therefore, the content of the amphoteric solubilizing emulsifier is preferably 0.3-3 weight percent of the complex lipid and 0.5-5 weight percent of the monoglyceride. In the practice of the present invention, when the amount of the complex lipid and monoglyceride-based amphoteric solubilizing emulsifier is within the above range, the amount of the two needs to be adjusted according to the compounding ratio. When the content ratio of the compound lipid to the monoglyceride substance is less than 0.2 or between 0.25-0.3, it will lead to excessive emulsification and poor system fluidity; when the content ratio of the compound lipid to the monoglyceride substance is greater than 0.3, the amount of the compound lipid is too large, the density difference of the system is large and stratification after emulsification occurs, and the emulsifier cannot completely disperse the mogroside; therefore, the content ratio of the compound lipid to the monoglyceride substance is preferably between 0.2-0.25.

[0107] From a microscopic perspective, the experimental principle of this invention is based on the adsorption and alignment behavior of emulsifiers at the oil-water interface. As a hydrophilic substance, mogroside has low solubility in oily media, so an emulsifier is needed to improve its dispersibility in the oil phase. Complex lipids and monoglycerides, acting as amphoteric solubilizing emulsifiers, can form a stable interfacial film at the oil-water interface, thereby promoting the uniform dispersion of mogroside in the oil phase.

[0108] The adsorption process of complex lipids and monoglycerides at the oil-water interface involves intermolecular van der Waals forces, hydrogen bonding, and hydrophobic interactions. Complex lipid molecules typically have long carbon chains, forming favorable hydrophobic interactions with medium-chain triglycerides in the oil phase. Monoglycerides, due to their hydrophilic groups, can form hydrogen bonds with mogroside molecules, thereby forming a stable interfacial film at the oil-water interface. When the ratio of complex lipids to monoglycerides is between 0.2-0.25, the two can work synergistically to form an interfacial film that is both sufficiently hydrophobic and hydrophilic, ensuring stable dispersion of mogroside in the oil phase.

[0109] Furthermore, the dispersion state of mogroside molecules in the oil phase is also related to their molecular structure. Mogroside molecules possess certain hydrophilic and hydrophobic properties. Therefore, at the oil-water interface, their hydrophilic groups can form hydrogen bonds with monoglycerides, while their hydrophobic groups face the oil phase, thereby achieving stable dispersion. When the ratio of complex lipids to monoglycerides is appropriate, a stable interfacial film can be formed, allowing the mogroside molecules to be evenly dispersed in the oil phase, avoiding aggregation and precipitation, thereby improving the stability and solubilization effect of the emulsion.

[0110] In summary, the present invention successfully achieves efficient solubilization and stable dispersion of mogroside in the oil phase by precisely controlling the content ratio of complex lipids and monoglycerides, as well as their adsorption and arrangement behaviors at the oil-water interface, providing a scientific basis for preparing high-quality mogroside-solubilized emulsions.

[0111] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit it. Although the present invention has been described in detail with reference to the above embodiments, ordinary technicians in the field should understand that the specific implementation methods of the present invention can still be modified or replaced by equivalents. Any modification or equivalent replacement that does not depart from the spirit and scope of the present invention should be covered by the scope of protection of the claims of the present invention.

Claims

1. A method for preparing a solubilized emulsion based on mogroside, characterized in that: The preparation method comprises: The composite amphiphilic emulsifier and the medium-chain triglyceride oily liquid are mixed, heated in a water bath, and stirred to obtain a binary mixed system; Adding amphiphilic solubilizing complex lipid to the binary mixed system and stirring evenly to obtain a ternary mixed system; adding mogroside to the ternary mixed system, heating in a water bath and stirring to obtain the mogroside-based solubilized emulsion; The weight ratio of the composite amphiphilic emulsifier, medium-chain triglyceride oily liquid, amphiphilic solubilizing compound lipid and mogroside is: (a) 4.28:94.23:0.99:0.5; or (b) 4.4:94.07:1.01:0.52; or (c) 3.71:94.68:1.11:0.5; or （d）4.11：94.36：1.02：0.51； The composite amphiphilic emulsifier is composed of mono- and diglycerol fatty acid esters and an emulsifier; The weight ratio of the mono- and di-glycerol fatty acid esters to the emulsifier is 2:1; The emulsifiers are selected according to the weight ratio: When the ratio is (a) or (c), the emulsifier is sucrose fatty acid ester; When the ratio is (b), the emulsifier is sorbitan fatty acid ester; When the ratio is (d), the emulsifier is xylitol monostearate.

2. The method for preparing the solubilized emulsion based on mogroside according to claim 1, characterized in that: When the composite amphiphilic emulsifier and the medium-chain triglyceride oily liquid are mixed and heated in a water bath with stirring, the heating temperature is 20-80°C.

3. The method for preparing the solubilized emulsion based on mogroside according to claim 1, characterized in that: Add mogroside to the ternary mixed system, heat in a water bath and stir at a heating temperature of 20-50°C.

4. A solubilized emulsion based on mogroside, characterized in that: The method for preparing the solubilized emulsion based on mogroside according to any one of claims 1 to 3 is prepared.

5. Use of the solubilized emulsion based on mogroside according to claim 4 in food additives.

6. A food additive, characterized in that The main component of the food additive is the solubilized emulsion based on mogroside according to claim 4.

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