Application of trans-ALPHA-bergamot glycerol and composition containing same in preventing fall armyworm from laying eggs

Abstract

The present invention relates to the use of trans-ALPHA-bergamot glycerol ene and a composition containing the same in repelling oviposition of fall armyworm. Among them, the composition containing trans-ALPHA-bergamot glycerol ene can produce a better technical effect in repelling oviposition of fall armyworm.

Description

Technical Field

[0001] The invention relates to the field of biological control, and in particular to application of trans-ALPHA-bergamot glycerol and a composition containing the same in repelling oviposition of fall armyworm. Background Art

[0002] Fall Armyworm Spodoptera frugiperda ) is a major invasive pest with a particularly wide range of host plants. It can feed on more than 76 families and more than 350 species of plants. Its larvae can eat a large number of crop leaves, causing serious economic losses. Among them, the fall armyworm is extremely harmful to corn and can cause a large-scale reduction in corn production. The female fall armyworm has a strong egg-laying ability, and each female can lay about 1,500 eggs in its lifetime. At present, most methods for preventing and controlling fall armyworms are chemical control, but in recent years, reports have begun to appear that fall armyworms have developed resistance to pesticides. At the same time, chemical pesticides will bring a series of problems such as environmental pollution and pesticide residues. In addition, the resistance of fall armyworms to Bacillus thuringiensis biocontrol agents has also been reported. It can be seen that traditional methods, whether chemical pesticides or genetically modified crops, will face severe challenges in controlling fall armyworms.

[0003] Currently, the biological control agents widely used in production include behavioral regulators, among which behavioral regulators mainly include sex attractants and food attractants, but sex attractants only attract male insects, and food attractants have poor sensitivity. Compounds that can repel female insects from laying eggs are called egg-laying repellents. The development of new green and efficient pest behavior regulators of this type can effectively reduce pest damage and has broad application prospects. Summary of the invention

[0004] One of the present invention provides trans-ALPHA-bergamot glycerol ene in repelling fall armyworm ( Spodoptera frugiperda ) application in spawning.

[0005] In a specific embodiment, the trans-ALPHA-bergamot glycerol is used to reduce the damage of fall armyworm to target plants.

[0006] In a specific embodiment, the target plant is at least one of corn, cotton and rice.

[0007] The second aspect of the present invention provides an egg-laying repellent composition, which comprises active components, wherein the active components include trans-ALPHA-bergamia triene, (3E)-4,8-dimethylnona-1,3,7-triene and linalool germacrene.

[0008] In a specific embodiment, the total mass of the active components is 100%, the mass of the trans-ALPHA-bergamot glycerol accounts for 29%, the mass of the (3E)-4,8-dimethylnona-1,3,7-triene accounts for 38%, and the mass of the linalool accounts for 33%.

[0009] In a specific embodiment, the egg-laying repellent composition further includes germacrene.

[0010] In a specific embodiment, based on the total mass of the active components as 100%, the mass proportion of germacrene is 10% to 20%.

[0011] The third aspect of the present invention provides an oviposition repellent composition according to the second aspect of the present invention for repelling fall armyworm ( Spodoptera frugiperda ) application in spawning.

[0012] In a specific embodiment, the egg-laying repellent composition is used to reduce damage to target plants by fall armyworm.

[0013] In a specific embodiment, the target plant is at least one of corn, cotton and rice.

[0014] Beneficial effects of the present invention: The present invention firstly discovers that trans-ALPHA-bergamot glycerol can repel fall armyworm ( Spodoptera frugiperda ) lay eggs, further, mixing trans-ALPHA-bergamot glycerol with (3E)-4,8-dimethylnona-1,3,7-triene and linalool in a mass ratio of 29:38:33 can produce a synergistic effect in repelling fall armyworm egg laying; and geranyl has a synergistic effect on the combination of trans-ALPHA-bergamot glycerol with (3E)-4,8-dimethylnona-1,3,7-triene and linalool. By applying (e.g., spraying or applying) or arranging (e.g., setting a device capable of releasing the egg-laying repellent composition at intervals) the egg-laying repellent composition (also referred to as egg-laying repellent) of the present invention on the target crop, the egg-laying amount of fall armyworm on the target crop can be reduced, so that not only can the purpose of reducing the damage to the target crop by fall armyworm be achieved, but also the use of chemical pesticides can be reduced, thereby promoting agricultural production, agricultural product quality and agricultural ecological safety, while effectively protecting natural enemy insects. Furthermore, based on the "push-pull" strategy, plants that attract the fall armyworm can be planted around the target crops, or attractants (such as plant-based attractants, sex attractants, etc.) can be used in the surrounding non-target crop areas to concentrate the killing of the attracted fall armyworms. This can more significantly reduce the population size of the fall armyworm, thereby effectively reducing the damage to the target crops and achieving the goal of green pest control. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 Shown is an oviposition behavior box for testing the effects of odor compounds on oviposition behavior of Spodoptera frugiperda.

[0016] Figure 2 The effects of the tested odor compounds on the oviposition behavior of Spodoptera frugiperda are shown. Different lowercase letters indicate significant differences between different odor compounds ( P <0.05, the same letters indicate no significant differences among the different tested odor compounds ( P >0.05). DETAILED DESCRIPTION

[0017] The above contents of the present invention are further described in detail below in the form of preferred implementation cases, but they do not constitute a limitation to the present invention.

[0018] Unless otherwise specified, the reagents in the embodiments of the present invention can be purchased through commercial channels. Example 1

[0019] Location: The experiment was conducted in the laboratory of Institute of Plant Protection, Chinese Academy of Agricultural Sciences.

[0020] Time: The test time is from 20:00 in the evening to 11:00 in the morning of the next day.

[0021] Indoor conditions: temperature is 26 to 28 degrees Celsius, relative humidity is 65±5%.

[0022] Trans-ALPHA-bergamot glycerol ene was mixed with a solvent, n-hexane, to prepare a 100 μg / μl mother solution, and then the mother solution of trans-ALPHA-bergamot glycerol ene was diluted to 0.1 μg / μl with n-hexane to prepare a trans-ALPHA-bergamot glycerol ene solution.

[0023] Use Figure 1 The egg-laying behavior box shown in the figure was used to test the effect of odor compounds on the egg-laying behavior of the fall armyworm. The specific operation sequence is as follows: Figure 1The open box at the top shown is used as an egg-laying behavior box; clean gauze is cut into two pieces of equal size (1 / 2 of the top area of ​​the egg-laying behavior box), and then one of the two pieces of gauze cut out with a size of 1 / 2 of the box top area is evenly soaked with 1 mL of 0.1 μg / μL trans-ALPHA-bergamot glycerol solution to obtain test odor (S1) gauze, and the other piece is evenly soaked with 1 mL of n-hexane to obtain n-hexane gauze, which is left for at least 1 min to allow the n-hexane on the gauze to evaporate; 3 egg-bearing female insects are placed in each egg-laying behavior box, and then the open top of the box is covered with two layers of gauze, and then the test odor gauze and n-hexane gauze are placed on top of the two layers of gauze, wherein the test odor gauze is placed on one side of the test odor, and the dose of trans-ALPHA-bergamot glycerol in the test odor gauze is 100 μg, and the n-hexane gauze is placed on the other side; the egg-laying amount is counted after 15 hours. One egg-laying behavior box was considered one replicate, for a total of five replicates.

[0024] The spawning index was calculated based on the spawning amount, and then the graph was plotted using Graphpad 9.5.1 software. In the figure, a solid circle represents a repeated spawning index, and N represents the number of repetitions. t -test analyzes the deterrent effect of the tested odor compound (trans-ALPHA-bergamot glycerol) on the fall armyworm, and compares whether there is a significant difference between the egg-laying index and 0. An egg-laying index less than 0 and with a significant difference from 0 indicates that the tested odor compound can significantly deter the fall armyworm from laying eggs. The smaller the egg-laying index, the better the deterrent effect.

[0025] Among them, the egg-laying index = (TC) / (T + C), C represents the egg-laying amount under the control n-hexane gauze, and T represents the egg-laying amount under the test odor gauze.

[0026] The results of the 5 replicate spawning indices are shown in Figure 2 . Example 2

[0027] The trans-ALPHA-bergamot glycerol in Example 1 was replaced with (3E)-4,8-dimethylnona-1,3,7-triene to obtain the test odor (S2) gauze, and the rest was the same as in Example 1.

[0028] The results of the 5 replicate spawning indices are shown in Figure 2 . Example 3

[0029] The trans-ALPHA-bergamot glycerol in Example 1 was replaced with linalool to obtain the test odor (S3) gauze, and the rest was the same as Example 1.

[0030] The results of the 5 replicate spawning indices are shown in Figure 2 . Example 4

[0031] The trans-ALPHA-bergamot glycerol in Example 1 was replaced with germacrene to obtain a test odor (S4) gauze, and the rest was the same as in Example 1.

[0032] The results of the 5 replicate spawning indices are shown in Figure 2 . Example 5

[0033] The trans-ALPHA-bergamot glycerol in Example 1 is replaced with a composition containing trans-ALPHA-bergamot glycerol, wherein the composition also includes (3E)-4,8-dimethylnona-1,3,7-triene and linalool. The mass ratio of trans-ALPHA-bergamot glycerol, (3E)-4,8-dimethylnona-1,3,7-triene and linalool in the composition is 29:38:33, and the total concentration of the three is 0.1 μg / μl, that is, the test odor M0 is obtained, and the rest is the same as in Example 1.

[0034] The results of the 5 replicate spawning indices are shown in Figure 2 . Example 6

[0035] The trans-ALPHA-bergamot glycerol in Example 1 is replaced with a composition containing trans-ALPHA-bergamot glycerol, wherein the composition also includes (3E)-4,8-dimethylnona-1,3,7-triene, linalool and germacene. The mass ratio of trans-ALPHA-bergamot glycerol, (3E)-4,8-dimethylnona-1,3,7-triene, linalool and germacene in the composition is 29:38:33:5, and the test odor M1 is obtained. The rest is the same as Example 5.

[0036] The results of the 5 replicate spawning indices are shown in Figure 2 . Example 7

[0037] The difference from Example 6 is that the mass ratio of trans-ALPHA-bergamot glycerol, (3E)-4,8-dimethylnona-1,3,7-triene, linalool and germacrene in the composition is 29:38:33:10, that is, the test odor M2 is obtained. The rest is the same as Example 5.

[0038] The results of the 5 replicate spawning indices are shown in Figure 2 . Example 8

[0039] The difference from Example 6 is that the mass ratio of trans-ALPHA-bergamot glycerol, (3E)-4,8-dimethylnona-1,3,7-triene, linalool and germacrene in the composition is 29:38:33:15, that is, the test odor M3 is obtained. The rest is the same as Example 5.

[0040] The results of the 5 replicate spawning indices are shown in Figure 2 . Example 9

[0041] The difference from Example 6 is that the mass ratio of trans-ALPHA-bergamot glycerol, (3E)-4,8-dimethylnona-1,3,7-triene, linalool and germacrene in the composition is 29:38:33:20, that is, the test odor M4 is obtained. The rest is the same as Example 5.

[0042] The results of the 5 replicate spawning indices are shown in Figure 2 . Example 10

[0043] The difference from Example 6 is that the mass ratio of trans-ALPHA-bergamot glycerol, (3E)-4,8-dimethylnona-1,3,7-triene, linalool and germacrene in the composition is 29:38:33:25, that is, the test odor M5 is obtained. The rest is the same as Example 5.

[0044] The results of the 5 replicate spawning indices are shown in Figure 2 .

[0045] according to Figure 2 The results show that the single trans-ALPHA-bergamot glycerol ( P <0.01), (3E)-4,8-dimethylnona-1,3,7-triene alone ( P <0.01) and linalool alone ( P <0.01) all had a deterrent effect on the oviposition of fall armyworm, while geranylgeranyl alone had no deterrent effect on the oviposition of fall armyworm ( P >0.05); further, after combining trans-ALPHA-bergamot glycerol, (3E)-4,8-dimethylnona-1,3,7-triene and linalool, it was found that the combination of the three can significantly improve (P<0.001) the deterrent effect on the oviposition of fall armyworm. Further, after combining geranylgeranyl with the three active components, it was found that geranylgeranyl at a certain dosage can significantly improve (P<0.001) the deterrent effect on the oviposition of fall armyworm, indicating that geranylgeranyl has a synergistic effect on the combination of the three components.

Claims

1. An egg-laying repellent composition, comprising active ingredients and germacene, wherein the active ingredients include trans-ALPHA-bergamotglycerol, (3E)-4,8-dimethylnona-1,3,7-triene and linalool; based on the total mass of the active ingredients as 100%, the mass proportion of germacene is 10% to 20%.

2. The egg-laying repellent composition according to claim 1, characterized in that: Taking the total mass of the active components as 100%, the mass of the trans-ALPHA-bergamot glycerol accounts for 29%, the mass of the (3E)-4,8-dimethylnona-1,3,7-triene accounts for 38%, and the mass of the linalool accounts for 33%.

3. The egg-laying repellent composition according to claim 1 or 2 is used to repel fall armyworm ( Spodoptera frugiperda ) application in spawning.

4. The use according to claim 3, characterized in that: The egg-laying repellent composition is used for reducing the damage of fall armyworm to target plants.

Images