Application of tomato saponin extract in preparation of medicine or functional food for improving visceral fat accumulation
Through the preparation process of high-content tomato saponin extract, the treatment problem of visceral fat accumulation is solved, the visceral fat index is significantly reduced, and the inhibition and improvement effect on visceral fat accumulation is achieved.
Patent Information
- Application Number
- CN202411402842.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-09
- Publication Date
- 2024-12-31
AI Technical Summary
The prior art has failed to effectively develop specific therapeutic drugs for visceral fat accumulation. Although tomato saponin extract has been used to lower lipids and anti-obesity, no relevant reports have been found for inhibiting or improving visceral fat accumulation.
Tomato saponin extract with a total saponin content of ≥50%, was prepared by solid-liquid separation of pectin enzyme solution, ultrafiltration membrane and nanofiltration membrane filtration, concentration and drying processes, and was used to prepare to improve perirenal and epididymis fat accumulation. Drugs or functional foods.
Tomato saponin extract effectively inhibits and improves the accumulation of visceral fat. It is used to prepare drugs or functional foods to improve visceral fat accumulation, significantly reducing the visceral fat index caused by high-fat feed.
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Figure CN119215101A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the field of medical technology, and in particular to application of a tomato saponin extract in preparing a medicine or functional food for improving visceral fat accumulation. Background Art
[0002] Visceral fat is a type of fat that, unlike subcutaneous fat, is mainly found in the abdominal cavity. A certain amount of visceral fat is actually necessary because it surrounds the internal organs and supports, stabilizes, and protects them. Excessive visceral fat is a manifestation of metabolic disorders in the body. Excessive accumulation of visceral fat can cause a variety of hazards, including an increased risk of cardiovascular disease, diabetes, cancer, and other diseases. Although many studies have been conducted on the pathogenesis, epidemiology, and natural history of visceral fat accumulation, no specific therapeutic drugs for visceral fat accumulation have been developed so far. Existing strategies for preventing and treating visceral fat accumulation mainly include controlling diet and increasing physical exercise.
[0003] Tomato is one of the fruits and vegetables we eat daily. It is rich in nutrition, has a special flavor, and contains rich saponin compounds and lycopene. Among them, esculeoside A is the main saponin component, and its content in the raw material is 3 times that of lycopene. In view of the multiple active ingredients in tomatoes, tomato extracts have been used for lipid reduction, lowering blood uric acid levels, and anti-atherosclerosis. In the invention patent with publication number CN104837336A, the use of tomato plant / fruit extracts in the preparation of a composition for preventing weight gain and / or inhibiting, preventing or improving obesity-related conditions in mammals is disclosed, but it is clearly stated that the tomatoes must be ripening-defective mutant tomatoes carrying ripening inhibition (rin), immature (nor) and / or never-mature (Nr) genes, such as selected from the variants "Sliimmy", "Mr Tasty", "GustoDelight" and "Tomango". According to the description of paragraphs 0058 to 0067 of the invention specification in combination with the drawings of the specification Figure 1It can be seen that the insulin-induced cells treated with the extract containing 0% rin have no effect on inhibiting adipogenesis in vitro. On the other hand, the invention points out that when extracting tomato plants / fruits, the solvent used is an organic solvent or a mixed solvent of water and an organic solvent, and lower alcohols are particularly preferred as solvents (paragraphs 0036 and 0037 of the specification); at the same time, paragraph 0033 of the specification further points out that the raw material for extraction is particularly preferably the peel (because it contains a large amount of components with anti-adipogenic activity), or the root of tomato, or the residue obtained after squeezing the tomato fruit (because it contains a large amount of peel). It can be seen that the component used to inhibit adipogenesis in the invention must not only be a ripening-defective mutant tomato carrying the ripening inhibition (rin), immature (nor) and / or never-mature (Nr) genes, but also an organic solvent extract of such a ripening-defective mutant tomato.
[0004] It is well known in the art that saponin compounds in tomatoes are water-soluble components. Currently, there are no reports on the use of tomato saponin extracts to inhibit / improve visceral fat accumulation. Summary of the invention
[0005] The technical problem to be solved by the present invention is to provide application of tomato saponin extract in preparing medicine or functional food for improving visceral fat accumulation.
[0006] The technical solution of the present invention is: use of tomato saponin extract in the preparation of medicine or functional food for improving visceral fat accumulation, wherein the total saponin content in the tomato saponin extract is ≥50%.
[0007] Specifically, it includes the use of tomato saponin extract in the preparation of a drug for improving perirenal fat accumulation, and the use of tomato saponin extract in the preparation of a drug for improving epididymal fat accumulation.
[0008] In the present invention, it is the saponin compounds in the tomato saponin extract that play the role of inhibiting / improving visceral fat accumulation. As long as the total saponin content in the tomato saponin extract meets the condition of being greater than or equal to 50%, there is no restriction on whether the raw tomato used for extraction carries the RIN gene.
[0009] Specifically, the tomato saponin extract is prepared from tomatoes using conventional methods. In the present application, it is preferably prepared according to the following method: solid-liquid separation of tomato pectinase hydrolyzate, collecting the liquid, first filtering it with an ultrafiltration membrane, filtering the permeate with a nanofiltration membrane, collecting the retained liquid, concentrating and drying it, and obtaining the extract.
[0010] Furthermore, when preparing the tomato saponin extract, the ultrafiltration membrane is an ultrafiltration membrane with a molecular weight cutoff of 2000Da, and the nanofiltration membrane is a nanofiltration membrane with a molecular weight cutoff of 500Da. The tomato pectinase hydrolyzate is obtained by pulping fresh tomatoes and adding pectinase for hydrolysis, and the amount of pectinase used is usually 0.04-0.06% of the weight of the raw material.
[0011] The applicant has found through experiments that water-soluble tomato saponin extract can effectively inhibit / improve the accumulation of visceral fat and can be used to prepare drugs or functional foods for improving the accumulation of visceral fat. BRIEF DESCRIPTION OF THE DRAWINGS
[0012] Figure 1 Effects of tomato saponin extract on visceral fat in mice. DETAILED DESCRIPTION
[0013] The following experiment further illustrates the effect of tomato saponin extract in inhibiting / improving the accumulation of visceral fat.
[0014] 1. Experimental Methods
[0015] 1.1 Preparation of tomato saponin extract
[0016] Take fresh tomatoes, wash them with clean water and dry them in the air, crush the tomatoes into pulp with a crusher and transfer them all to the enzymolysis tank, add 0.05% pectinase by weight of the fresh tomato sample, stir well and enzymolyze at 50°C for 2h. The enzymolysis solution is first coarsely filtered with a filter cloth of 80 mesh, and the filtrate is collected and centrifuged (5000rpm for 10min). After centrifugation, the supernatant is collected and the residue is discarded to obtain a crude extract of tomato saponin. The crude extract is firstly removed from the macromolecular impurities such as proteins and lipids with a larger pore size (molecular weight cutoff of 2000Da), and the permeate is collected; then the ultrafiltration permeate is filtered with a nanofiltration membrane with a relatively small pore size (molecular weight cutoff of 500Da), blocking the tomato saponin with a molecular weight of more than 500 from passing through, and allowing impurities with smaller molecular weights such as glucose and fructose to pass through; the retained feed liquid in the nanofiltration is collected and evaporated and concentrated to obtain a tomato saponin extract. By evaporative light scattering-high performance liquid chromatography (chromatographic conditions: chromatographic column ZORBAX SB-C18 column; column temperature 30°C; mobile phase methanol-water gradient elution; detection time 20 min; flow rate 0.8 mL / min; injection volume 10 μL; evaporative light scattering detector parameters drift tube temperature 100°C; air flow rate: 2.7 L / min), the content of total saponins in the obtained tomato saponin extract was 55.6%.
[0017] 1.2 Animal Experiment Design
[0018] After 7 days of adaptive feeding of mice, 12 mice were randomly selected according to body weight as the normal feed group and 30 mice as the high-fat feed group. The mice in the normal feed group were given normal control feed, and the mice in the high-fat feed group were given high-fat feed for 8 consecutive weeks. After 8 weeks, all mice were weighed, and 24 mice whose weight exceeded 20% of the average weight of mice in the normal feed group were selected from the high-fat feed group for subsequent experiments. 12 mice in the normal feed group were used as the normal feed control group, and 24 mice selected from the high-fat feed group were randomly divided into a high-fat feed control group and a tomato saponin extract group according to body weight, with 12 mice in each group. The normal feed control group continued to be given normal control feed, and the high-fat feed control group and the tomato saponin extract group were given high-fat feed. The tomato saponin extract group was given 400 mg / kg of tomato saponin extract by gavage every day, and the normal feed control group and the high-fat feed control group were given the same dose of distilled water by gavage every day for 8 consecutive weeks. The mice were weighed once a week, and the dosage of tomato saponin extract was adjusted according to body weight.
[0019] 1.3 Experimental Animal Treatment
[0020] After 8 weeks of administration, the mice were anesthetized with sodium pentobarbital, and blood was collected after they lost consciousness. The mice were then euthanized, and epididymal fat and perirenal fat were collected and accurately weighed to calculate the visceral fat index. Visceral fat index = visceral fat weight × 1000 / mouse weight, where visceral fat weight = epididymal fat weight + perirenal fat weight (mouse visceral fat is generally represented by epididymal fat + perirenal fat).
[0021] 1.4 Statistics
[0022] All experimental data are expressed as mean ± standard deviation, and the data were statistically analyzed using GraphPad Prism 8 (San Diego, CA, USA) software. Outliers and missing data were excluded from the analysis. The normality of data distribution was assessed using the Kolmogorov-Smirnov test, and the equality of variance was assessed using the Bartlett test. For comparisons between multiple groups, one-way analysis of variance was used, followed by post hoc comparisons using the Tukey test. p < 0.05 was considered statistically significant.
[0023] 2. Experimental results
[0024] The experimental results are shown in Table 1 and Figure 1 shown.
[0025] Table 1. Effects of tomato saponin extract on visceral fat in mice
[0026]
[0027] The experimental data are expressed as mean ± standard deviation (n = 12), and different superscripts (a, b, c) in each group indicate significant differences (p < 0.05).
[0028] From Table 1 and Figure 1 It can be seen that the epididymal fat weight, perirenal fat weight, visceral fat weight and visceral fat index of mice in the high-fat diet control group were significantly higher than those in the normal diet control group (p<0.05), indicating that mice eating a high-fat diet will cause visceral fat accumulation. The epididymal fat weight, perirenal fat weight, visceral fat weight and visceral fat index of mice in the tomato saponin extract group were significantly lower than those in the high-fat diet control group (p<0.05), indicating that tomato saponin extract can improve visceral fat accumulation caused by a high-fat diet.
Claims
1. Application of tomato saponin extract in the preparation of medicine or functional food for improving visceral fat accumulation, wherein: The total saponin content in the tomato saponin extract is ≥50%.
2. The use according to claim 1, characterized in that Application in the preparation of drugs for improving perirenal fat accumulation.
3. The use according to claim 1, characterized in that Application in the preparation of medicines for improving epididymal fat accumulation.
4. The use according to any one of claims 1 to 3, characterized in that: The tomato saponin extract is prepared by the following method: solid-liquid separation of tomato pectinase hydrolyzate, collecting the liquid, first filtering it with an ultrafiltration membrane, filtering the permeated liquid with a nanofiltration membrane, collecting the intercepted liquid, concentrating and drying it, and obtaining the tomato saponin extract.
5. The use according to any one of claims 1 to 3, characterized in that: The ultrafiltration membrane is an ultrafiltration membrane with a molecular weight cutoff of 2000 Da, and the nanofiltration membrane is a nanofiltration membrane with a molecular weight cutoff of 500 Da.