Application of calmodulin-binding protein CBP60g or its encoding gene CBP60g in clubroot prevention

By overexpressing CBP60g in Arabidopsis thaliana and rapeseed, the problem of clubroot disease control under high temperature was solved, the plant's disease resistance and immune response were enhanced, and normal growth and yield were not affected. This provides a molecular breeding program under climate change.

CN119220582BActive Publication Date: 2026-06-02HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HUAZHONG AGRI UNIV
Filing Date
2024-09-24
Publication Date
2026-06-02

AI Technical Summary

Technical Problem

Under high temperature conditions, clubroot disease severely affects cruciferous crops such as Arabidopsis thaliana and rapeseed, and existing technologies are insufficient to effectively control it, thus impacting yield.

Method used

By overexpressing calmodulin-binding protein CBP60g or its encoding gene CBP60g in Arabidopsis thaliana and rapeseed, the disease resistance of plants under high temperature was enhanced. Overexpression vectors containing CBP60g, such as pGWB517, were constructed to promote its expression under high temperature conditions.

Benefits of technology

Enhancing plant resistance to clubroot bacteria and restoring the resilience of immune responses under high temperatures without affecting normal plant growth and yield provides a molecular breeding strategy for addressing climate change.

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Abstract

The application belongs to the field of agricultural biotechnology, and particularly relates to application of a calmodulin-binding protein CBP60g or a coding gene CBP60g thereof in control of clubroot, wherein an amino acid sequence of the calmodulin-binding protein CBP60g is shown as SEQ ID NO. 1. The application can restore the resistance of Arabidopsis thaliana to Plasmodiophora brassicae at high temperature by overexpressing CBP60g, and the same is verified in Brassica napus, and the normal growth, yield and quality of the Brassica napus are not affected, which indicates that the CBP60g gene can resist Plasmodiophora brassicae at high temperature, and increase the recovery of the immune response of crops to high-temperature combined stress, and provides a strategy for molecular breeding in response to climate change.
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