A mild exfoliating brightening composition and use thereof

Abstract

This invention belongs to the field of cosmetic technology, specifically relating to a gentle exfoliating and brightening composition and its application. Specifically, this invention uses a combination of protease aqueous solution, probiotic-fermented brown algae, and panthenol to prepare a composition; then, using this composition as the main active ingredient, it is prepared into a skincare cosmetic. Experimental verification has shown that it can effectively restore the skin's microecological balance, while also brightening the skin. Furthermore, compared to conventional acid-based exfoliating ingredients like salicylic acid, it has a stronger exfoliating effect, a more pronounced exfoliating effect, and is gentler, thus possessing significant practical application value.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a gentle exfoliating and brightening composition and its application. Background Technology

[0002] The information disclosed in the background section of this invention is intended only to enhance the understanding of the overall background of the invention and is not necessarily to be construed as an admission or in any way implying that such information constitutes prior art known to those skilled in the art.

[0003] Salicylic acid, an extract from willow bark, is a fat-soluble hydroxy acid and a natural anti-inflammatory agent (the commonly used drug aspirin is a derivative of salicylic acid, sodium acetylsalicylate). As a fat-soluble substance, salicylic acid can penetrate into lipid-rich pores, combating acne caused by excessive sebum secretion. It can also be used to remove excess keratin, helping to restore skin smoothness. Unlike other acidic substances such as fruit acids, salicylic acid is not highly irritating. This is because the human stratum corneum itself contains abundant lipids, and salicylic acid's fat-soluble nature allows it to fuse with the lipids of the stratum corneum, penetrating deep into the stratum corneum and pores without irritating the dermis. Its effect is locked in the superficial stratum corneum, without affecting active epidermal cells, exhibiting high stability and low irritation.

[0004] While salicylic acid is relatively mild compared to other acids, people with sensitive skin still experience irritation when using salicylic acid products. In fact, excessive use of salicylic acid can worsen an already sensitive skin barrier. Therefore, developing an ingredient / composition that can replace salicylic acid for gentle exfoliation without damage, and even brighten and repair the skin, is a pressing issue in this field. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this invention provides a gentle exfoliating and brightening composition and its applications. Specifically, this invention uses a combination of protease aqueous solution, probiotic-fermented brown algae, and panthenol to prepare a composition; then, using this composition as the main active ingredient, it is prepared into a skincare cosmetic. Experimental verification has shown that it can effectively restore the skin's microecological balance, while also providing a brightening effect. Furthermore, compared to conventional acid-based exfoliating ingredients like salicylic acid, it exhibits stronger exfoliating efficacy, a more pronounced exfoliating effect, and is gentler. Based on the above research findings, this invention is thus completed.

[0006] To achieve the above-mentioned technical objectives, the present invention provides the following technical solution:

[0007] A first aspect of the present invention provides a mild peeling and brightening composition, said composition comprising the following components in parts by weight:

[0008] 0.01–10 parts of protease aqueous solution, 0.1–30 parts of probiotic-fermented brown algae, and 0.01–5 parts of panthenol.

[0009] The protease content in the aqueous protease solution is 0.1-1.5%, preferably 1%; further, the protease is a serine proteolytic enzyme, and even more preferably a keratinase.

[0010] The probiotic-fermented brown algae is obtained through fermentation with multiple food-grade probiotics. In one specific embodiment of the present invention, the probiotic-fermented brown algae is a seaweed fermentation liquid obtained using the fermentation method in CN 108653059 A. The brown algae is kelp. It should be noted that the probiotic-fermented brown algae (seaweed fermentation liquid) does not contain live bacteria.

[0011] In another specific embodiment of the present invention, the composition comprises the following components in parts by weight:

[0012] One part protease aqueous solution, six parts probiotic-fermented brown algae, and two parts panthenol.

[0013] A second aspect of the invention provides the use of the above-described composition in the preparation of cosmetics.

[0014] The cosmetic product has at least one or more of the following effects:

[0015] (a) Skin barrier repair;

[0016] (b) Radiant skin;

[0017] (c) Balance the skin's microecology.

[0018] A third aspect of the invention provides a cosmetic product with barrier repair, brightening, and skin microecological balancing effects, said cosmetic product comprising at least the above-described composition. More specifically, said cosmetic product can be a skin care product.

[0019] According to the cosmetic of the present invention, the amount of the composition added is 0.5-15% based on the total mass of the cosmetic, preferably 2-10%, such as 2%, 3%, 5%, 7%, 8%, 9% or 10%.

[0020] The cosmetics may also contain any other ingredients permitted in the cosmetics industry, including but not limited to emulsifiers, emollients, humectants, thickeners, and preservatives.

[0021] Furthermore, by rationally adding the above-mentioned raw material components, this invention can also be used to prepare different cosmetic dosage forms, such as aqueous solutions, emulsions, and creams. In addition, based on the above-mentioned basic cosmetic categories, other cosmetic categories can be further derived and prepared, such as facial cleansers, face creams, toners, and face masks, which are not specifically limited here.

[0022] A fourth aspect of the present invention provides a method for preparing the above-described cosmetic, the method comprising the step of mixing the composition with other raw material components.

[0023] The beneficial technical effects of one or more of the above technical solutions are as follows:

[0024] (1) The above technical solution provides a mild peeling and brightening composition with certain barrier repair effects, which can promote FLG gene expression and has certain synergistic effects among the components.

[0025] (2) Compared with conventional acid peeling ingredient salicylic acid, the composition obtained by the above technical solution not only does not damage the microecological balance of facial skin, but can also restore the microecological balance. In addition, the inventors unexpectedly discovered that the composition of the present invention can also further brighten the skin by promoting certain specific bacteria to produce specific metabolites, thus achieving multiple effects and reducing product costs.

[0026] (3) Compared with the conventional acid-based exfoliating ingredient salicylic acid, it has a stronger exfoliating effect, a more obvious exfoliating effect, and is gentler. Attached Figure Description

[0027] The accompanying drawings, which form part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments of the invention and their descriptions are used to explain the invention and do not constitute an improper limitation of the invention.

[0028] Figure 1 This invention is a test of the inhibitory effect of different samples on Staphylococcus epidermidis.

[0029] Figure 2 The results are from the test of exfoliated keratinocytes in the aqueous solution of the composition in Example 1 of this invention. Detailed Implementation

[0030] It should be noted that the following detailed description is illustrative and intended to provide further explanation of the invention. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0031] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of exemplary embodiments according to the invention. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0032] The present invention will now be further illustrated with specific examples. These examples are for illustrative purposes only and do not limit the scope of the invention. Unless otherwise specified, experimental conditions not explicitly stated in the examples are generally performed under conventional conditions or as recommended by the reagent company. Unless otherwise specified, all reagents and consumables used in the following examples are commercially available.

[0033] The following examples further illustrate the present invention, but do not constitute a limitation thereof. It should be understood that these examples are for illustrative purposes only and not for limiting the scope of the invention. Test methods not specifically described in the following examples are generally performed under conventional conditions. The 1% aqueous solution of the protease (keratinase) in the examples and comparative examples of the present invention was purchased from Givaudan Fragrance & Flavor (Shanghai) Co., Ltd. The probiotic fermented brown algae was the seaweed fermentation broth obtained using the method described in Example 1 of CN 108653059A. The salicylic acid inclusion complex was purchased from Shandong Binzhou Zhiyuan Biotechnology Co., Ltd. Panthenol was purchased from BASF (China) Co., Ltd.

[0034] The specific components of Examples 1 to 6 are shown in the table below:

[0035]

[0036]

[0037] The specific compositions of comparative examples 1 to 8 are shown in the table below.

[0038]

[0039] Application Example 1: Preparation of Toner

[0040] Phase A (parts by weight): 4 parts glycerol, 4 parts butylene glycol, 0.4 parts succinate, 0.4 parts hexanediol, 0.05 parts EDTA2Na, deionized water To 100;

[0041] Phase B: 1 part protease aqueous solution (1%), 6 parts probiotic fermented brown algae, 2 parts panthenol

[0042] The preparation steps for the above-mentioned toner are as follows:

[0043] 1) Add glycerol, butylene glycol, succinate, hexanediol, EDTA2Na and deionized water to a beaker, heat and stir in a 65°C water bath to dissolve them completely, and obtain phase A;

[0044] 5) When the emulsion temperature drops to 40-45℃, add phase B to the system and stir at 40 r / min to cool it down;

[0045] 6) When the temperature drops to 38°C, the lotion containing the composition of Example 1 is obtained by static cooling.

[0046] Application Example 2: Emulsion Preparation

[0047] Phase A (parts by weight): 1 part A165 (emulsifier), 3 parts GTCC, 3 parts white oil, 0.5 parts cetearyl alcohol, 5 parts silicone oil;

[0048] Phase B (parts by weight): 4 parts glycerin, 4 parts butylene glycol, 0.4 parts EMT-10 (thickener), 0.1 parts xanthan gum, 0.4 parts succinate, 0.4 parts hexanediol, 0.2 parts soybean lecithin, 100 parts deionized water;

[0049] Phase C: 1 part of protease aqueous solution (1%), 6 parts of probiotic fermented brown algae, and 2 parts of panthenol.

[0050] The emulsion preparation steps are as follows:

[0051] 1) First, disperse EMT-10, xanthan gum and soybean lecithin into glycerol and butylene glycol, then add acetone, hexanediol and deionized water, heat and stir in an 80°C water bath to fully dissolve them, and obtain phase B;

[0052] 2) Melt A165, GTCC, white oil, cetearyl alcohol, and silicone oil in a water bath at 83°C to obtain phase A;

[0053] 3) Heat phase A and phase B in a water bath at 83°C. When the two phases are at the same temperature, slowly add phase A to phase B while stirring phase B at 500 r / min.

[0054] 4) After phase B and phase A are mixed, the emulsion is cooled while stirring at 430 r / min.

[0055] 5) When the emulsion temperature drops to 40-45℃, add phase C to the system and stir at 60 r / min to cool it down;

[0056] 6) When the temperature drops to 38°C, static cooling is performed to obtain an emulsion containing the composition of Example 1.

[0057] Experiment Example 1: In Vitro Repair Efficacy Test

[0058] (a) Cell seeding: After cell resuscitation, when the cell seeding rate reaches about 60%, seed the cells into 6-well plates and incubate overnight in a CO2 incubator (37°C, 5% CO2).

[0059] (ii) Solution preparation: Prepare working solutions of test substances according to test groups.

[0060]

[0061]

[0062] Note: The aqueous solutions in Examples 1-6 and Comparative Examples 1-8 were prepared by adding water to bring the volume up to 100g.

[0063] (III) Drug Administration: According to the test groups, when the cell deposition rate in the 6-well plate reached 40%–60%, drug administration was performed in groups, with 3 replicates per group. 2 mL of culture medium was added to each well of the blank control group, 2 mL of culture medium containing WY14643 was added to each well of the positive control group, and 2 mL of culture medium containing the corresponding concentration of the test sample was added to each well of the sample group. After drug administration, the 6-well plate was placed in a CO2 incubator (37℃, 5% CO2) for 24 h.

[0064] (iv) Cell collection: After incubation, remove the old solution, wash twice with PBS, add 1 mL of RNAiso Plus to each well, lyse the cells by pipetting, and collect the sample.

[0065] (v) Gene expression detection: RNA was extracted, reverse transcribed into cDNA, and then detected by real-time PCR. The results were calculated using the 2-ΔΔCT method.

[0066] (vi) Calculation of upward adjustment rate:

[0067] (vii) FLG gene test results

[0068] Serial Number Group Upward adjustment rate (vs BC) Significance 1 Blank group (BC) / / 2 Positive control (PC) 28.00％ ** 3 Example 1: Aqueous Solution of the Composition 88.10％ ** 4 Example 2: Aqueous Solution of the Composition 72.24％ ** 5 Example 3: Aqueous Solution of the Composition 90.56％ ** 6 Example 4: Aqueous Solution of the Composition 96.25％ ** 7 Example 5: Aqueous Solution of the Composition 112.76％ * 8 Example 6: Aqueous Solution of the Composition 98.63％ ** 9 Comparative Example 1: Aqueous Solution of the Composition 23.86％ ** 10 Comparative Example 2: Aqueous Solution of the Composition 50.16％ * 11 Comparative Example 3: Aqueous Solution of the Composition 41.16％ * 12 Comparative Example 4: Aqueous Solution of Composition 15.75％ ** 13 Comparative Example 5: Aqueous Solution of the Composition 10.25％ * 14 Comparative Example 6: Aqueous Solution of Composition 20.16％ ** 15 Comparative Example 7: Aqueous Solution of Composition 22.46％ ** 16 Comparative Example 8: Aqueous Solution of Composition 1.02％ /

[0069] Note: Significance compared to group BC is indicated by *, P-value < 0.05 is indicated by *, P-value < 0.01 is indicated by **.

[0070] Experimental Example 2: Staphylococcus epidermidis (ATCC12228) test

[0071] 1) Culture medium preparation: TSB liquid medium: 1.5% tryptone (g / 100ml), 0.5% soybean peptone (g / 100ml), 0.5% sodium chloride (g / 100ml), with the remainder being distilled water, and the pH adjusted to 7.2±0.2. Sterilize by pressure steaming at 121℃ for 20 min. TSB solid medium is prepared by adding 2% (g / 100ml) agar powder to the above medium.

[0072] 2) Bacterial resuscitation: Under aseptic conditions, use a pipette to take a small amount of thawed Staphylococcus epidermidis bacterial solution and drop it near the edge of a solid culture medium. Inoculate the medium using the streak method and incubate upside down at 37°C for 24 hours.

[0073] 3) Preparation of bacterial suspension: Using a sterile pipette tip, pick a single colony from the solid culture medium and place it into 1 mL of liquid culture medium. Incubate at 37℃ in a constant temperature shaker (200 r / min) for 24 h. Determine the concentration of the bacterial suspension using the dilution method; the concentration should be 1 × 10⁻⁶. 6 CFU / mL.

[0074] 4) Sample culture: Under aseptic conditions, add 720 μL of liquid culture medium, 8 μL of bacterial suspension, and 180 mg of sample to a 1.5 mL centrifuge tube, mix well, and replace the sample with physiological saline in the blank control group. Perform three parallel experiments for each sample, and incubate all samples in a 37℃ constant temperature shaker (200 r / min) for 24 h.

[0075]

[0076] 5) Dilution method for determining bacterial count: Remove the sample from the shaker, take 20 μL of the sample, add 180 μL of liquid culture medium, and mix well. This is a 10-fold dilution. Take 20 μL of the diluted solution, add 180 μL of liquid culture medium, and mix well. This is a 10-fold dilution. 2 Dilute to 10 times using this method. 5 Pour the solid culture medium onto plates, label them, and then dilute it 10 times. 2 ~10 5 Take 5 μL of each sample to be tested and add it to the corresponding position on the plate. Incubate at 37°C with the plate upside down for 24 hours. Observe the bacterial growth.

[0077] Depend on Figure 1 It can be seen that, compared with the blank group No. 1, sample No. 15 (containing only salicylic acid) has a certain inhibitory effect on Staphylococcus epidermidis. Compared with No. 14, sample No. 14 (containing salicylic acid) has a certain inhibitory effect on Staphylococcus epidermidis. The sample provided by the present invention can promote the proliferation of Staphylococcus epidermidis, and its proliferation efficiency is higher than that of single or two-component combinations, which is more conducive to the balance of skin microecology.

[0078] Experimental Example 3: Evaluation of a 3D Melanocyte Skin Model

[0079] (1) Experimental Instruments and Materials: Mela 3D melanocyte skin model, batch number: EM221201, Guangdong Boxi Biotechnology Co., Ltd.; MelaGrowth melanocyte culture medium, batch number: 230621, Guangdong Boxi Biotechnology Co., Ltd.; α-arbutin, 100g / pack, Guangzhou Tingnan Biotechnology Co., Ltd.; phosphate-buffered saline (PBS), Gibco; sodium hydroxide, Guangzhou Chemical Reagent Factory; dimethyl sulfoxide, Sinopharm Chemical Reagent Co., Ltd.; biosafety cabinet, NUNRE; carbon dioxide incubator, SHELLLAB (USA); colorimeter, Minolta; multi-functional microplate reader, Thermo Fisher Scientific; constant temperature water bath, Shanghai Yiheng Scientific Instruments Co., Ltd.

[0080] (2) Experimental methods

[0081] ① Test substance: The survival rate of B16-F10 mouse melanoma cells treated with Experimental Examples 1-7 and Comparative Examples 1-8 for 24 hours was not less than 92% (under the experimental concentration conditions, it did not have cytotoxicity). 1 ml of sample was taken, and the solvent was melanocyte culture medium Mela Growth.

[0082] ② Skin model preparation: Define the day the skin model is received as DAY 0. Prepare a 6-well plate, add 0.9 mL of resuscitation medium (MelaGrowth melanocyte culture medium) to each well, and transfer qualified models to the 6-well plate using sterile forceps. Incubate at 37°C, 5% CO2, and 95% relative humidity for 1 hour. After incubation, discard the resuscitation medium, replace it with the same volume of resuscitation medium, and incubate overnight.

[0083] ③ Sample Addition and Detection: Thirty-three skin models were naturally melanized, forming a model group and a sample group. The model group consisted of three untreated skin models, while the sample group consisted of thirty skin models. Three parallel skin models were used for each detection point, and the average value was taken. Except for the model group, 1 ml of each of experimental cases 1-3 and control cases 1-7 was applied to the surface of the sample group daily for 24 hours, for three days, with the melanocyte culture medium (MelaGrowth) changed daily. Three models from the model group and thirty models from the sample group were then used for testing.

[0084] ④ Melanin content determination: Place the skin model in a 1.5 mL centrifuge tube, add 1 mL of PBS buffer, vortex for 3 min, discard the liquid, then add 1 mL of 1 mol / L NaOH solution containing 10% DMSO to the 1.5 mL centrifuge tube, incubate at 80℃ for 40 min, mix well, and then transfer 200 μL to a 96-well plate. Read the absorbance value at 405 nm using a microplate reader.

[0085] Table 1: Results of melanin content

[0086]

[0087] Note: **Compared with the model group, P < 0.01.

[0088] The melanin content results on day 3 are shown in Table 1. Compared with the model group, the melanin content decreased after the composition of the present invention was applied for 24 hours daily for three consecutive days, and the differences were statistically significant (P < 0.05). As can be seen from Table 1 above, the composition of the present invention has a significant melanin inhibition effect compared with single components and pairs of compound components, and also has a certain synergistic whitening effect.

[0089] Experiment Example 4: External Exfoliation Efficacy Test

[0090] (I) Test Grouping

[0091]

[0092] (II) Administration

[0093] 1) Fix the skin between the supply chamber and the receiving chamber of the Franz cell diffusion cell, with the stratum corneum facing the supply chamber and the dermis facing the receiving chamber. Add 7.0 mL of receiving solution to the receiving chamber, tighten and fix the excised skin tissue, and add 1.0 mL of receiving solution (PBS) to the receiving chamber through the sampler. Remove the air and make the dermis of the skin in close contact with the receiving solution.

[0094] 2) Add 50 μL of sample to the skin surface in the supply chamber (PBS buffer was added to the surface of the excised skin tissue as a blank control). Spread the sample evenly from the center of the skin outwards, with an exposed sample area of ​​3.14 cm2. Each sample was tested in triplicate and parallel. The bath was kept at (32±1)℃, and the water bath was kept free of air bubbles.

[0095] 3) After incubation for 24 hours, put on a finger cot and rub the sample application area. After rubbing for 2 minutes, add 0.5 mL of cleaning solution (0.1% Triton X-100) to the supply chamber and blow to clean the sloughed keratinocytes on the skin surface.

[0096] 4) Transfer the cleaning solution to a 1.5 mL centrifuge tube for later use.

[0097] (III) Microscopic photography of exfoliated keratinocytes

[0098] 1) Shake the eluted keratinocyte suspension to mix well, use a pipette to draw 10 μL of keratinocyte suspension and drop it into the center of an adhesive glass slide, then spread the droplet with the pipette tip.

[0099] 2) Place the slide on an automatic slide baking machine, set the baking temperature to 50℃, and bake for 10 minutes to dry the liquid and fix the keratinocytes on the slide.

[0100] 3) Add sodium fluorescein staining solution to the glass slide, so that the staining solution evenly covers the smear, and stain at room temperature in the dark for 10 minutes.

[0101] 4) After staining, rinse with PBS until the wash water is colorless. Then place the slide in the washing box and wash twice with PBS, 5 minutes each time.

[0102] 5) Add an appropriate volume of anti-fluorescence quencher to the smear and seal it.

[0103] 6) Place the slide under a fluorescence microscope and take pictures under a 20× objective lens.

[0104] (iv) Total protein content detection

[0105] 1) Place the collected cleaning solution into a high-speed centrifuge, set the speed to 15000 rpm, centrifuge for 10 min, precipitate the keratinocytes in the cleaning solution, discard the supernatant, and resuspend the precipitate in 0.5 mL of deionized water.

[0106] 2) Add 25 μL of sodium hydroxide (12M) solution to the resuspended liquid, lyse the keratinocytes in a boiling water bath for 30 min, and add 25 μL of concentrated hydrochloric acid (12M) to neutralize the lysis buffer.

[0107] 3) Detect the total protein content according to the instructions of the BCA protein assay kit.

[0108] (V) Calculation of Lifting Rate:

[0109] (vi) Test Results

[0110] 1) Results of the exfoliated keratinocyte test

[0111] The results are as follows Figure 2 As shown, more fluorescent green indicates more shed keratinocytes. Compared to group BC, the number of shed keratinocytes in group PC and the aqueous solution of Example 1 increased significantly, indicating that both group PC and the aqueous solution of Example 1 can promote the shedding of keratinocytes and have exfoliating effects. Meanwhile, the number of shed keratinocytes in the aqueous solution of Example 1 was significantly higher than that in group PC, indicating that the aqueous solution of Example 1 provided by this invention has better exfoliating effects than salicylic acid.

[0112] 2) Total protein content test results

[0113]

[0114] Note: When performing statistical analysis using the t-test method, significance compared to group BC is indicated by *, P-value < 0.05 is indicated by *, and P-value < 0.01 is indicated by **.

[0115] Compared with group BC, the total protein content of the aqueous solution of Example 1 was significantly increased. Based on isolated pig skin, the total protein content of the aqueous solution of Example 1 was significantly increased compared with the control group, indicating that the number of exfoliated keratinocytes was significantly increased. This suggests that the sample can promote the exfoliation of keratinocytes and has an exfoliating effect, and its exfoliating ability is superior to that of salicylic acid.

[0116] The above embodiments are only for illustrating the technical concept and features of the present invention, and are intended to enable those skilled in the art to understand the content of the present invention and implement it accordingly. They should not be construed as limiting the scope of protection of the present invention. All equivalent changes or modifications made in accordance with the spirit and essence of the present invention should be covered within the scope of protection of the present invention.

Claims

1. A mild exfoliating lightening composition characterized in that, The composition comprises components in the following mass fractions: 0.01-10 parts of a proteinase aqueous solution, 0.1-30 parts of probiotic fermented kelp, and 0.01-5 parts of panthenol; The proteinase content in the proteinase aqueous solution is 0.1-1.5%; The probiotic fermented kelp is obtained by the following fermentation method, specifically: 1) Dry kelp is soaked in purified water, and 1 part of kelp is taken with 15 parts of water; the dry kelp is repeatedly washed with purified water after being fully soaked to remove the dirt and salt on the surface of the kelp; 2) The clean kelp is dried at low temperature, and the dried kelp is ultra-finely pulverized to a particle size of less than 50 μm; 3) The strains used for fermentation are activated, wherein the strains used for fermentation are Bacillus amyloliquefaciens, Bacillus licheniformis, Saccharomyces boulardii, Saccharomyces cerevisiae, Saccharomyces uvarum, Lactobacillus acidophilus, and Lactobacillus confusus; 4) The low-temperature dried kelp is irradiated for sterilization at 1000-2500 kilorad for 2 hours; 5) The activated Bacillus amyloliquefaciens is cultured in a fermentation medium, and the medium formula is 1 L of distilled water + 20 g of glucose + 15 g of peptone + 5 g of sodium chloride + 0.5 g of beef extract; after the Bacillus amyloliquefaciens is cultured to a cell viable count of 109 cfu / mL, the ultra-finely pulverized and sterilized kelp powder is inoculated into the fermentation broth at a proportion of 5%, and the Bacillus amyloliquefaciens is continuously cultured; 6) The kelp powder is degraded by various enzymes produced by the growth of the Bacillus amyloliquefaciens, and the kelp and the culture medium gradually become a fermented kelp liquid with high viscosity; after 24 hours of fermentation, the fermentation is stopped; 7) After the fermentation is completed, the Bacillus amyloliquefaciens is removed by centrifugation at 6000 rpm / min for 10 minutes, and then the Saccharomyces boulardii 1%, the Saccharomyces cerevisiae 2%, and the Saccharomyces uvarum 2% are inoculated at a total inoculum of 5% for secondary fermentation, and the fermentation condition is static culture at 30 degrees for 72 hours; 8) After the yeast fermentation is completed, the lactic acid bacteria are inoculated at an inoculum of 5% for third fermentation, and the fermentation condition is static culture at 30 degrees for 48 hours; 9) After the mixed fermentation of the three strains is completed, the fermentation broth is placed in a low-temperature environment of 10 degrees for post-ripening conversion, and the post-ripening time is 15 days; 10) After the post-ripening is completed, the liquid is centrifuged, and the supernatant is collected, and the centrifugation condition is centrifugation at 10000 rpm / min for 10 minutes; 11) The centrifuged supernatant is filtered by a 0.22 um filter membrane, and the obtained supernatant is a seaweed fermentation broth; The kelp is kelp; The proteinase is keratinase; The probiotic fermented kelp does not contain live bacterial components.

2. The composition of claim 1, wherein The composition comprises components in the following mass fractions: 1 part of a proteinase aqueous solution, 6 parts of probiotic fermented kelp, and 2 parts of panthenol.

3. Use of the composition of any one of claims 1-2 in the preparation of a cosmetic product.

4. The use according to claim 3, wherein the compound is ###0002### The cosmetic product has at least any one or more of the following effects: (a) skin barrier repair; (b) skin brightening; (c) skin microecological balance.

5. A cosmetic product having the effect of barrier repair, brightening, and balancing the skin microflora, characterized in that, The cosmetic product at least comprises the composition of any one of claims 1-2.

6. The cosmetic product according to claim 5, wherein The cosmetic product is a skin care product.

7. The method for producing a cosmetic according to claim 5 or 6, characterized in that, The preparation method comprises the step of mixing the composition with other raw material components.

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