Bacillus subtilis Sneb2016, culture, biological control product, method and application

Abstract

The invention provides bacillus subtilis Sneb2016, a culture, a biological control product, a method and application, and particularly belongs to the technical field of microbial pesticides. The bacillus subtilis Sneb2016 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.30519. The bacillus subtilis Sneb2016 has the effect of inducing tomatoes to generate resistance to the root knot nematode disease, can be prepared into a biological control fungicide for preventing and treating the tomato root knot nematode disease, effectively prevents and treats meloidogyne incognita infestation, has a remarkable effect, can promote plant growth, and has a good application prospect in prevention and treatment of the tomato root knot nematode disease. Good application prospects are realized in the aspect of preventing and treating tomato root knot nematode diseases.

Description

Technical Field

[0001] The invention belongs to the technical field of microbial pesticides, and specifically relates to a strain of Bacillus subtilis Sneb2016, a culture, a biological control product, a method and an application thereof. Background Art

[0002] Root-knot nematodes (Meloidogynes pp.) are an important type of plant parasitic nematodes with a wide range of hosts, more than 3,000 plant species, and often cause serious economic losses. In recent years, with the continuous expansion of greenhouse vegetable planting areas, its occurrence has also increased year by year. Tomato root-knot nematode disease is caused by southern root-knot nematodes and is one of the main diseases in tomato production in the world. The prevention and control methods of tomato root-knot nematode disease are mainly based on the selection of disease-resistant varieties and chemical control. The use of chemical fungicides can easily cause pathogens to develop drug resistance, pollute the environment, and endanger the ecological balance. Biological control with the characteristics of good prevention effect, non-toxicity, and no pollution has also become a research hotspot for scholars. However, there are not many reports on biological control of tomato root-knot nematode disease. Although the prior art has reported some biological control bacteria that have a preventive and control effect on southern root-knot nematodes, these bacteria need to be irrigated during the prevention and control process, and the amount used is large. Therefore, there is a lack of a more efficient biological control bacteria for preventing and controlling tomato root-knot nematode disease. Summary of the invention

[0003] The object of the present invention is to provide a strain of Bacillus subtilis Sneb2016, a culture, a biological control product, a method and an application thereof. The present invention provides a strain of bacteria that can induce resistance to tomato root-knot nematode disease, which can be used to produce a biological control product for controlling tomato root-knot nematode disease by inducing resistance, such as inducing tomato roots to produce resistance to infection and development of southern root-knot nematodes by coating tomato seeds, which has a significant effect, can reduce the amount of bacteria used and simplify the control operation.

[0004] The present invention provides a Bacillus subtilis Sneb2016, wherein the Bacillus subtilis Sneb2016 is deposited in the General Microbiological Center of China National Microbiological Culture Collection Administration, and the deposit number is CGMCC No.30519.

[0005] The present invention also provides a culture of Bacillus subtilis Sneb2016.

[0006] Preferably, the culture comprises a fermentation broth, a metabolic broth or an extract.

[0007] The present invention also provides a method for culturing the Bacillus subtilis Sneb2016 culture described in the above technical solution, comprising the following steps: culturing Bacillus subtilis Sneb2016 in a culture medium to obtain a Bacillus subtilis Sneb2016 culture.

[0008] The present invention also provides the use of the Bacillus subtilis Sneb2016 described in the above technical scheme or the culture of Bacillus subtilis Sneb2016 described in the above technical scheme in preventing and controlling southern root-knot nematode disease of plants and / or promoting plant growth.

[0009] Preferably, the application method comprises one or more of root irrigation, seed coating and seed soaking.

[0010] The present invention also provides a method for preventing and controlling southern root-knot nematode disease in plants, comprising the following steps:

[0011] The Bacillus subtilis Sneb2016 described in the above technical solution or the culture of Bacillus subtilis Sneb2016 described in the above technical solution is used to treat plants; the treatment includes one or more of seed coating treatment, seed soaking treatment and root irrigation treatment.

[0012] The present invention also provides the use of the Bacillus subtilis Sneb2016 described in the above technical scheme or the culture of the Bacillus subtilis Sneb2016 described in the above technical scheme in inducing tomato resistance to southern root-knot nematodes.

[0013] The present invention also provides a method for inducing tomato to resist southern root-knot nematodes, comprising the following steps:

[0014] The Bacillus subtilis Sneb2016 described in the above technical solution or the culture of Bacillus subtilis Sneb2016 described in the above technical solution is used to coat the seeds of plants and / or soak the seeds.

[0015] The present invention also provides a biological control product for controlling southern root-knot nematode disease of plants, the biological control product comprising the Bacillus subtilis Sneb2016 described in the above technical solution or the culture of Bacillus subtilis Sneb2016 described in the above technical solution, and an auxiliary material acceptable to the biological control product.

[0016] The present invention provides a strain of Bacillus subtilis Sneb 2016. The Bacillus subtilis Sneb 2016 of the present invention has the function of inducing tomato to produce resistance to root-knot nematode disease, can be prepared into a biological control bacterial agent for preventing and controlling tomato root-knot nematode disease, can effectively prevent and control southern root-knot nematode infection, has a significant effect, and can promote plant growth, and has good application prospects in preventing and controlling tomato root-knot nematode disease.

[0017] Biological deposit information

[0018] Bacillus subtilis Sneb2016 is deposited in the General Microbiology Center of China Microorganism Culture Collection Administration, referred to as CGMCC, and the deposit date is May 7, 2024. The address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, and the deposit number is CGMCC No.30519. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0020] Figure 1 The colony morphology of Bacillus subtilis Sneb2016 provided by the present invention;

[0021] Figure 2 The bacterial body and attached flagella of Bacillus subtilis Sneb2016 provided by the present invention;

[0022] Figure 3 A phylogenetic relationship diagram of Bacillus subtilis Sneb2016 and other closely related strains provided by the present invention. DETAILED DESCRIPTION

[0023] The present invention provides a strain of Bacillus subtilis Sneb2016, and the Bacillus subtilis Sneb2016 is deposited in the General Microbiology Center of China National Microbiological Culture Collection Administration, with a deposit number of CGMCC No. 30519. In a specific embodiment, the Bacillus subtilis Sneb2016 of the present invention can be cultured on LB medium. In a specific embodiment, the LB medium includes LB solid medium and liquid medium. In a specific embodiment, after the Bacillus subtilis Sneb2016 is cultured on LB solid medium under dark conditions for 24 hours, the surface of the colony is smooth, milky white, and sticky when picked up; a single cell is 0.7-0.8×2-3 microns, evenly colored; no capsule, peritrichous flagella, and can move, such as Figure 1 and Figure 2As shown. Bacillus subtilis Sneb2016 is Gram-positive and is a facultative anaerobe. It can utilize sucrose and glucose, but is citrate-negative. In the present invention, the nucleotide sequence of 16S rDNA of Bacillus subtilis Sneb2016 is shown in SEQ ID NO.1. A homology search and comparison was performed in NCBI, and a homology search was performed in GenBank using the BLASTN 2.2.31 system. It was found that the strain with the highest sequence similarity was Bacillus subtilis, with a sequence similarity of 99%, indicating that the strain belongs to Bacillus subtilis. The present invention takes tomato root-knot nematode disease as a target, and finds biocontrol bacteria with the ability to induce resistance to southern root-knot nematodes from the screening of biocontrol bacteria, thus finding new resources for the research and development of new biological nematicides. In a specific embodiment, the Bacillus subtilis Sneb2016 or its culture thereof of the present invention has the effect of inducing resistance of tomatoes to root-knot nematodes, and can be used to produce biocontrol products for controlling tomato root-knot nematodes by inducing resistance. By coating or soaking tomato seeds, the tomato root system can be induced to produce resistance to infection and development of southern root-knot nematodes, with significant effects. At the same time, it can also promote the growth of tomatoes. It has good application prospects in the field of controlling tomato root-knot nematodes or in the preparation of biocontrol agents for controlling tomato root-knot nematodes.

[0024] The present invention also provides a culture of Bacillus subtilis Sneb 2016. In a specific embodiment, the culture includes a fermentation liquid, a metabolic liquid or an extract.

[0025] The present invention also provides a method for culturing Bacillus subtilis Sneb2016 described in the above technical solution, comprising the following steps: culturing Bacillus subtilis Sneb2016 in a culture medium to obtain a culture of Bacillus subtilis Sneb2016. In a specific embodiment, before the culturing, the strain can be activated. The present invention does not specifically limit the method for strain activation, and a conventional strain activation method can be used. The present invention does not specifically limit the type of culture medium, and any culture medium that can achieve the growth of Bacillus subtilis Sneb2016 can be used, such as LB culture medium or NA culture medium; it can be a solid culture medium or a liquid culture medium, such as LB solid culture medium or LB liquid culture medium. In a specific embodiment, each liter of the culture medium contains 5 to 20 g of tryptone, 3 to 10 g of yeast extract, and 5 to 40 g of sodium chloride. In a specific embodiment, each liter of the culture medium contains 10 g of tryptone, 5 g of yeast extract, and 10 g of sodium chloride. In a specific embodiment, the culture medium may also include agar. After the culture medium is prepared, the present invention also includes sterilizing the culture medium. In a specific embodiment, the sterilization condition can be sterilized at 121°C for 30 minutes. In a specific embodiment, the culture temperature is 20-28°C, which can be 20°C, 22°C, 25°C, 27°C or 28°C. In a specific embodiment, the culture speed is 150-160r / min. In a specific embodiment, the culture time is 1-3d, which can be 2d. In the present invention, the culture includes fermentation liquid, metabolic liquid or extract. When the culture is fermentation liquid, the bacterial concentration in the fermentation liquid is 0.5×10 8 cfu / mL to 1×10 8 cfu / mL.

[0026] The present invention also provides the use of the Bacillus subtilis Sneb2016 described in the above technical solution or the culture of Bacillus subtilis Sneb2016 described in the above technical solution in preventing and treating southern root-knot nematode disease of plants and / or promoting plant growth. In a specific embodiment, the plant includes tomato. In a specific embodiment, the method of application includes one or more of root irrigation, seed coating and seed soaking.

[0027] The present invention also provides a method for preventing and controlling southern root-knot nematode disease in plants, comprising the following steps: treating plants with the Bacillus subtilis Sneb2016 described in the above technical solution or the culture of the Bacillus subtilis Sneb2016 described in the above technical solution; the treatment comprises one or more of seed coating treatment, seed soaking treatment and root irrigation treatment. In a specific embodiment, the plant comprises a tomato.

[0028] The present invention also provides the use of the Bacillus subtilis Sneb2016 described in the above technical solution or the culture of the Bacillus subtilis Sneb2016 described in the above technical solution in inducing tomato resistance to southern root-knot nematodes. In a specific embodiment, the induction method includes seed coating treatment and / or seed soaking treatment.

[0029] The present invention also provides a method for inducing tomato to resist southern root-knot nematodes, comprising the following steps:

[0030] The Bacillus subtilis Sneb2016 described in the above technical solution or the culture of Bacillus subtilis Sneb2016 described in the above technical solution is used to coat the seeds of plants and / or soak the seeds.

[0031] The present invention also provides a biological control product for controlling southern root-knot nematode disease in plants, the biological control product comprising the Bacillus subtilis Sneb2016 described in the above technical solution or the culture of Bacillus subtilis Sneb2016 described in the above technical solution, and an adjuvant acceptable to the biological control product. In the present invention, the biological control product can be a coating agent, a seed dressing agent or a seed soaking agent.

[0032] In order to further illustrate the present invention, a strain of Bacillus subtilis Sneb2016, culture, biological control product, method and application provided by the present invention are described in detail below in conjunction with the accompanying drawings and examples, but they should not be construed as limiting the scope of protection of the present invention.

[0033] Example 1

[0034] 1. Colony morphology and microbiological characteristics

[0035] The colony morphology of Bacillus subtilis Sneb2016 is as follows Figure 1 shown.

[0036] After culturing on LB solid medium in the dark for 24 hours, the surface of the colony is smooth, milky white, and sticky when picked up. The single cell is 0.7-0.8×2-3 microns, with uniform coloring. It has no capsule, flagella around it, and can move. Figure 2 shown.

[0037] The culture conditions of the Bacillus subtilis Sneb2016 are as follows:

[0038] Lysing broth agar medium (LB) solid culture medium (1L) formula: 10g tryptone, 5g yeast extract, 10g sodium chloride, 17-20g agar, 1000mL distilled water, sterilized at 121℃ for 30min.

[0039] Natural pH.

[0040] Slant culture method: culture at 28℃ for 24h.

[0041] 2. Physiological and biochemical characteristics

[0042] Bacillus subtilis Sneb2016 is Gram-positive and is a facultative anaerobe. It can utilize sucrose and glucose, but is citrate-negative. Other characteristics are shown in Table 1:

[0043] Table 1 Physiological and biochemical characteristics of strain Sneb2016

[0044]

[0045]

[0046] 3. Gene structure characteristics

[0047] The 16S rDNA sequence of the Bacillus subtilis Sneb2016 was amplified by PCR and sequenced, and the result is shown in SEQ ID NO.1:

[0048] CGGGCGGGGCTATACATGCAGTCGAGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGGTTGTTTGAACCGCATGGTTCAAACATAAAAGGTGGCTTCGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGATGAAGGTTTTCGGATCGTAAAGCTCTGTTGTTAGGGAAGAACAAGTACCGTTCGAATAGGGCGGTACCTTGACGGTACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGCAGGCGGTTTCTTAAGTCTGATGTGAAAGCCCCCGGCTCAACCGGGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGAAGAGGAGAGTGGAATTCCACGTGTAGCGGTGAAATGCGTAGAGATGTGGAGGAACACCAGTGGCGAAGGCGACTCTCTGGTCTGTAACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAACGATGAGTGCTAAGTGTTAGGGGGTTTCCGCCCCTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACGGTCGCAAGACTGAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGTCTTGACATCCTCTGACATCCTAGAGATAGGACGTCCGCTTCGGGGCAGAGTGACAGTGGTGCATGGTGTCGTCAGCTCGTGTCGTGAGATGTGGGTAGTCCCGCAACGAGCGCAACCCTGATCTAGTTGCCAGCATTCGTTGGGCACCTCTAAGTGACTGCCGTGACAAACCGCAGAAGGTGGGAT.

[0049] The 16S rDNA sequences were searched and compared in NCBI for homology, and the BLASTN2.2.31 system was used to search for homology in GenBank. It was found that the strain with the highest sequence similarity was Bacillus subtilis, with a sequence similarity of 99%, indicating that the strain belongs to Bacillus subtilis. The phylogenetic tree of Bacillus subtilis Sneb2016 is shown in Figure 1. Figure 3 shown.

[0050] Example 2

[0051] A method for preparing a fermentation liquid for preventing and controlling tomato root-knot nematode disease by using Bacillus subtilis Sneb2016.

[0052] The method is specifically as follows: after activating the test tube strain of Bacillus subtilis Sneb2016, inoculate it into a 250ml triangular flask (each bottle contains 150ml LB liquid fermentation medium) for cultivation, the culture conditions are: 20-28°C, 150-160r / min shaking table fermentation for 2 days, and obtain a fermentation liquid with induced resistance to tomato root knot nematode disease.

[0053] Example 3

[0054] The toxicity of fermentation broth prepared from Bacillus subtilis Sneb2016 against southern root-knot nematode J2 was determined.

[0055] 1. Preparation of root-knot nematode suspension

[0056] The pathogenic nematodes were collected from the roots of diseased plants in the root-knot nematode breeding room of the Northern Nematode Research Institute of Shenyang Agricultural University. They were identified as southern root-knot nematodes and artificially inoculated and propagated using living tobacco plant roots. The vigorous J2-stage larvae were selected for activity testing.

[0057] 2. Preparation of Bacillus subtilis Sneb2016 fermentation broth

[0058] After the test tube strain of Bacillus subtilis Sneb2016 was activated, it was inoculated into a 250 mL triangular flask (each bottle contained 150 mL LB liquid fermentation medium) for culture. The culture conditions were as follows: 20-28 ° C, 150-160 r / min shaking fermentation for 2 days, the bacterial solution was centrifuged and the supernatant was removed, resuspended with sterile water, and the OD value was adjusted to 1.0 with a spectrophotometer. The bacterial concentration in the fermentation broth was 1×10 8 cfu / mL. A fermentation liquid with induced resistance to tomato root-knot nematode disease was obtained.

[0059] 3. Toxic activity of Bacillus subtilis Sneb2016 against southern root-knot nematodes

[0060] Take 0.1mL of Bacillus subtilis Sneb2016 fermentation broth and mix it thoroughly with 0.1mL of nematode solution (i.e. the bacterial test concentration is 0.5×10 8 cfu / mL), add 0.2mL to each well, add 3 wells (i.e. 3 times of repetition), and use sterile water as control. After covering, place it at 24℃ in the dark and investigate the number of dead nematodes at 6h, 12h, 18h, and 24h. Investigation method: investigate each well (replicate), count 6 times, investigate the number of dead and live nematodes. The total number of nematodes investigated for each treatment is 90 to 110.

[0061] Corrected mortality rate (%) = (treatment mortality rate - control mortality rate) / (100 - control mortality rate) × 100% Criteria for determining the death of nematodes: When observing nematodes under a microscope, nematodes that are active or curled in an "S" shape can be determined as alive, and nematodes that are stiff and motionless or in a "J" shape are determined to be inactive and dead. When it is difficult to determine, use a pick to gently touch the worm body. If the worm body does not react at all, it is determined to be dead. The results are shown in Table 2.

[0062] Table 2 Toxic activity of strain Sneb2016 against southern root-knot nematodes

[0063]

[0064] Example 4

[0065] Experiment on the preparation of metabolites of Bacillus subtilis Sneb2016 to control tomato root-knot nematode disease 1. Preparation of test preparations

[0066] The fermentation liquid of metabolites obtained by preventing and controlling tomato root-knot nematode disease prepared in the above Example 2 is set aside.

[0067] 2. Tested tomato varieties and southern root-knot nematodes

[0068] The experimental tomato varieties and southern root-knot nematodes were provided by the Northern Nematode Research Institute of Shenyang Agricultural University.

[0069] 3. Potted plant protection test of indoor strain Sneb2016 against tomato root-knot nematode disease

[0070] The concentration was prepared to 1.0×10 8 cfu / mL of Bacillus subtilis Sneb2016 fermentation liquid were sown in plastic pots, with 3 tomato seeds per pot, and blank medium was used as control, with 5 replicates. Normal cultivation was carried out in a solar greenhouse. When the tomatoes grew to the two-leaf stage, one tomato plant with the same growth was left in each pot and inoculated with 2000 southern root-knot nematodes per plant. 25 days after inoculation, the root disease was investigated, the number of root knots was calculated, and the plant height, root length, plant fresh weight and underground fresh weight were measured.

[0071] The test results are shown in Table 3:

[0072] From the results of potted plant control efficacy test in Table 3, it can be seen that the incidence and severity of tomato root-knot nematode disease in tomato plants treated with strain Sneb2016 were significantly lower than those in the blank control. The treatment had the best control effect on tomato root-knot nematode disease, with the number of root knots being 50, which was 68.29% lower than that of the control treatment, indicating that strain Sneb2016 can induce resistance in tomatoes and reduce the infestation of southern root-knot nematodes. This result is significantly different from the previous biological agents and chemical nematicides for killing nematodes. The amount of fermentation liquid used in seed treatment is very low, but the efficiency is very high, which is the feature of the present invention. The plant height and root length of tomato plants treated with strain Sneb2016 were also significantly higher than those of the control, increasing by 23.72% and 9.13% respectively.

[0073] Table 3 Potted plant protection test of indoor Bacillus subtilis Sneb2016 against tomato root knot nematode disease

[0074] deal with Number of root knots Plant height / cm Root length / cm Fresh weight per plant / g Root fresh weight / g Sneb2016 (50±3.0)b (40.00±1.00)b (16.37±0.93)b (6.07±0.38)b (2.45±0.05)b CK (157.67±8.5)a (32.33±2.08)a (15.00±0.92)a (5.4±0.4)a (1.89±0.06)a

[0075] The cost of controlling tomato root-knot nematode by poisoning is very high. Controlling tomato root-knot nematode in greenhouse costs hundreds or even thousands of yuan per mu. The method of inducing tomato roots to produce resistance to root-knot nematodes by seed treatment of the present invention can greatly reduce the cost of control and improve economic benefits.

[0076] 4. Field efficacy test of strain Sneb2016 against tomato root-knot nematode disease

[0077] This experiment was conducted in Qianshanheying Village, Xinmin Township, Taihe District, Jinzhou City, Liaoning Province. Field plot design: 80m long, 6m wide, 0.5m ridge spacing, 25 to 30 tomato seedlings planted per ridge. Tomato seedlings were cultured after being coated with strain Sneb2016 at a fermentation liquid to seed weight ratio of 1:40, and blank culture medium treatment was used as the control. There were 2 groups of treatments, each with 3 replicates, and protective rows around the plot. At 30 days, 5 seedlings were randomly selected from each replicate plot of each treatment to investigate the root knot index. Root knot index grading standard: The disease level is determined based on the percentage of the area occupied by the root knot on the root to the total root area, expressed by the grading method, with 1% to 20%, 20% to 40%, 40% to 60%, 60% to 80% and 80% to 100% root knot index as 1, 2, 3, 4 and 5 respectively, and the leaves are not diseased, recorded as "0". During the investigation, the severity of the disease in each investigated root was estimated visually. At maturity, five tomato plants were randomly selected from each treatment to measure plant height, root length, plant fresh weight and fresh weight of underground parts.

[0078] Table 4 Field efficacy test of strain Sneb2016 coating treatment for tomato root-knot nematode disease

[0079]

[0080] The results of the field test are shown in Table 4:

[0081] The results in Table 4 show that in terms of the incidence of tomato root-knot nematode disease in the field, the root knot index of the strain Sneb2016 treatment was 1.33 and 1.50, respectively, which was 64.81% and 57.14% lower than the blank control. The plant height and root length of tomato plants treated with strain Sneb2016 were also significantly higher than those of the control, with plant height increased by 6.20% and 14.25%, respectively, and root length increased by 41.89% and 9.54%, respectively.

[0082] The above experiments show that the Bacillus subtilis Sneb2016 provided by the present invention can effectively prevent and control tomato root-knot nematode disease with significant effect, and can also promote tomato growth. It has good application prospects in preventing and controlling tomato root-knot nematode disease, and can be made into a biological control agent for preventing and controlling tomato root-knot nematode disease.

[0083] Using seed treatment to induce resistance to tomato root-knot nematode disease is a new field of green prevention and control. Seed immunity induction is low-cost, easy to operate, and does not pollute the environment. It is a new direction for plant protection research. The mechanism of the present invention is to regulate the metabolism of tomato roots so that the roots can be changed to resist nematode infection. This method breaks through the timeliness and spatial limitations of traditional soil treatment. The method of killing nematodes with poison on a large root system requires a large amount of nematicides or biological agents, while seed immunity-induced resistance is a good solution for preventing and controlling tomato root-knot nematode disease.

[0084] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A strain of Bacillus subtilis Sneb2016, characterized in that: The Bacillus subtilis Sneb2016 is deposited in the General Microbiology Center of China Microorganism Culture Collection Administration, with the deposit number being CGMCC No.30519.

2. A culture of Bacillus subtilis Sneb2016.

3. The culture according to claim 2, characterized in that The culture includes fermentation liquid, metabolic liquid or extraction liquid.

4. The method for culturing the Bacillus subtilis Sneb2016 culture according to claim 2 or 3, characterized in that: The method comprises the following steps: culturing Bacillus subtilis Sneb2016 in a culture medium to obtain a culture of Bacillus subtilis Sneb2016.

5. Use of the Bacillus subtilis Sneb2016 described in claim 1 or the culture of Bacillus subtilis Sneb2016 described in claim 4 in preventing and controlling southern root-knot nematode disease of plants and / or promoting plant growth.

6. The use according to claim 5, characterized in that: The application method comprises one or more of root irrigation, seed coating and seed soaking.

7. A method for preventing and controlling southern root-knot nematode disease in plants, characterized in that: The following steps are involved: The Bacillus subtilis Sneb2016 described in claim 1 or the culture of Bacillus subtilis Sneb2016 described in claim 4 is used to treat plants; the treatment includes one or more of seed coating treatment, seed soaking treatment and root irrigation treatment.

8. Use of the Bacillus subtilis Sneb2016 according to claim 1 or the culture of Bacillus subtilis Sneb2016 according to claim 4 in inducing resistance of tomatoes to southern root-knot nematodes.

9. A method for inducing resistance of tomatoes to southern root-knot nematodes, characterized in that: The following steps are involved: The Bacillus subtilis Sneb2016 culture according to claim 1 or the Bacillus subtilis Sneb2016 culture according to claim 4 is used to perform seed coating treatment and / or seed soaking treatment on plants.

10. A biological control product for controlling southern root-knot nematode disease in plants, characterized in that: The biological control product comprises the Bacillus subtilis Sneb2016 according to claim 1 or the culture of Bacillus subtilis Sneb2016 according to claim 4, and an auxiliary material acceptable to the biological control product.

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