Thermobifida fusca detection method and kit
CN119287049BActive Publication Date: 2026-08-28HEAVYBIO INC
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Patent Information
- Application Number
- CN202411570326.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-05
- Publication Date
- 2026-08-28
- Estimated Expiration
- 2044-11-05
AI Technical Summary
Technical Problem
目前针对热噬淀粉芽孢杆菌的检测方法主要是细菌分离培养和质谱技术，但该检测方法耗时长，检测过程比较繁琐
Benefits of technology
[0011]综上，本发明以热噬淀粉芽孢杆菌（Bacillus thermoamylovorans）的16SrDNA、gyrA或lipase基因作为检测靶基因，提供了一种用于热噬淀粉芽孢杆菌（Bacillusthermoamylovorans）核酸扩增的引物组，以及相应地用于检测热噬淀粉芽孢杆菌的核酸检测方法及试剂盒。本发明的检测方法灵敏度高（最低检出限可以达到100cfu/mL）、特异性好、可重复性强、检测结果快速同时能降低污染。本发明克服了现有的针对热噬淀粉芽孢杆菌的检测方法耗时长，过程繁琐等缺陷，在热噬淀粉芽孢杆菌的检测领域展现出广阔的应用潜力，能够用于牛奶等乳制品的快速检测。
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Abstract
The present application relates to a primer set for nucleic acid amplification of Bacillus thermoamylovorans, and a nucleic acid detection method and kit for rapidly and sensitively detecting Bacillus thermoamylovorans accordingly. The present application overcomes the defects of the existing detection methods for Bacillus thermoamylovorans, such as long time consumption, complicated process, etc., and provides a detection method with high sensitivity, good specificity, strong repeatability, rapid detection result and reduced pollution. The present application shows broad application potential in the field of Bacillus thermoamylovorans detection.
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