Application of (E)-2-hexenal in rice anti-chilo suppressalis inducer and inducer

Abstract

The invention belongs to the technical field of agricultural biology, and relates to a rice anti-chilo suppressalis induction technology, in particular to application of (E)-2-hexenal in a rice anti-chilo suppressalis inducer and the inducer. The inducer is a solution containing an effective component, the effective component contains (E)-2-hexenal, and a solvent of the solution is a volatile solvent. The rice anti-chilo suppressalis inducer is loaded on a carrier, a release device capable of releasing (E)-2-hexenal is prepared, the release device is placed in a rice field, and the release device is used for releasing volatile matter containing (E)-2-hexenal to induce rice defense and guard. The application of (E)-2-hexenal in the rice anti-chilo suppressalis inducer and the inducer provided by the invention have important significance in effectively preventing and treating chilo suppressalis and ensuring the rice product and quality safety. The invention provides a green and safe solution for preventing and treating chilo suppressalis in rice, and has wide application value and wide ecological benefits.

Description

Technical Field

[0001] The present invention belongs to the field of agricultural biotechnology, and relates to a rice resistance to Chilo suppressalis induction technology, and specifically relates to ( E Use of )-2-hexenal as a rice resistance inducer against Chilo suppressalis and the inducer. Background Art

[0002] As one of the most important food crops, rice is cultivated in more than 30 million hectares in my country. The Chilo suppressalis is a rice-boring pest that causes extremely high damage and frequency of occurrence on rice, causing rice sheaths, heart or panicles to wither, which seriously restricts rice production. In recent years, due to the continuous innovation of rice planting patterns and the increase in the resistance of the Chilo suppressalis to pesticides, the degree of damage has been on the rise. In addition, people's awareness of food safety and environmental protection has been continuously improving. Therefore, it is necessary to take green and safe measures to prevent and control the Chilo suppressalis to ensure the safety of rice yield and quality.

[0003] Studies have shown that when attacked by herbivorous insects, plants will immediately produce and release a large amount of volatile substances, namely herbivore-induced plant volatiles (HIPVs), to resist pests by directly repelling pests or attracting natural enemies. In addition to being a "chemical signal" to mediate the interaction between plants and insects, a large number of studies in recent years have also shown that HIPVs can be perceived and recognized by neighboring plants as a "warning signal", allowing them to quickly enter the "warning state". When pest stress comes, plants in the "warning state" will show faster response ability, stronger insect resistance and tolerance. Recently, the internationally renowned journal New Phytologist published the team's research paper titled "Stemborer-induced rice plant volatiles boost direct and indirect resistance in neighboring plants" as a cover article online. The paper systematically studied the ecological regulatory function of pest-induced volatiles in the process of information exchange between rice plants. The results showed that compared with rice plants exposed to healthy rice volatiles, rice plants exposed to rice volatiles induced by Chilo suppressalis showed faster expression of genes related to jasmonic acid and protease inhibitor pathways in rice plant tissues after subsequent Chilo suppressalis infestation, with significantly increased expression levels, and the content of jasmonic acid and protease inhibitors also increased, significantly inhibiting the growth and development of Chilo suppressalis larvae. However, it is still unclear which volatiles play a key role in regulating Chilo suppressalis-induced rice interactions.

[0004] Therefore, determining which volatiles can enhance the defense response of rice, and then using the volatiles as rice resistance inducers and inducers is a technical problem to be solved by the present invention. Solving this problem is of great significance for effectively controlling the rice stem borer and ensuring the safety of rice products and quality. Summary of the invention

[0005] Based on this, the object of the present invention is to provide a ( E )-2-hexenal is used as a rice anti-striped stem borer inducer and an inducer. The active component of the inducer contains ( E )-2-hexenal, releases ( E The volatiles of )-2-hexenal can induce rice defense alert. This effective component is green and safe, can be used in pollution-free production, and has broad ecological benefits.

[0006] To achieve the above object, the present invention adopts the following technical solutions: The present invention provides a (E) -2-hexenal is used as a rice anti-striped stem borer inducer, and the effective component of the rice anti-striped stem borer inducer contains (E) -2-Hexenal.

[0007] Furthermore, the effective components of the rice anti-striped stem borer inducer are (E) -2-Hexenal single component.

[0008] Furthermore, the rice resistance to Chilo suppressalis inducer is a solution containing the effective component, and the solvent of the solution is a volatile solvent.

[0009] Furthermore, the volatile solvent is selected from any one of dichloromethane, n-hexane and acetone.

[0010] Furthermore, the solution (E) -The molar ratio of 2-hexenal to solvent is 1.5~150μg / μL.

[0011] Furthermore, the solution (E) The amount ratio of 2-hexenal to the solvent is 10-30 μg / μL, more preferably 14-16 μg / μL, for example 15 μg / μL.

[0012] Furthermore, the rice anti-striped stem borer inducer is loaded on a carrier to be made into a carrier capable of releasing (E) -2-hexenal release device, the carrier is a container or an adsorbent.

[0013] Furthermore, the release device is placed in a rice field, and the release device is used to release the (E) Volatiles of 2-hexenal induce defense alert in rice.

[0014] Any of the above-mentioned rice resistance to Chilo suppressalis inducers is also a technical solution to be induced by the present invention.

[0015] The rice resistance to Chilo suppressalis inducing agent provided by the present invention may also include any one or more of a surfactant, an adhesive, a dispersant, a propellant, a stabilizer, an insecticide, an insect repellent or a synergist.

[0016] The beneficial effects of the present invention are: The present invention provides ( E ) - Use of 2-hexenal as a rice anti-striped stem borer inducer and inducer, with ( E )-2-hexenal is the active component of the inducer, which exists in rice itself, is green and safe, can be used in pollution-free production, and has broad ecological benefits. The inducer can induce the rice plant to be on guard, and can start its own defense response faster and stronger when the rice stem borer is infested. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solution of the present invention, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0018] Attached Figure 1 It is a rice resistance inducer against Chilo suppressalis ( E )-Schematic diagram of the release device of 2-hexenal; Attached Figure 2 The data diagram of the release rate of the rice anti-striped stem borer inducer of different concentrations in the release device in Example 1; Attached Figure 3 This is a comparison chart of the release rate of the inducer at a concentration of 15 μg / μL in Example 1 and the release rate of rice volatiles after being infested by the Chilo suppressalis for five days; Attached Figure 4 Box plot of the weight growth of Chilo suppressalis larvae on rice with and without the treatment of Chilo suppressalis inducer; Attached Figure 5 The graph shows the changes in the content of plant hormone jasmonic acid in rice tissues treated with or without the Chilo suppressalis inducer. Attached Figure 6 The graph shows the changes in trypsin inhibitor content in rice tissues treated with and without Chilo suppressalis inducer. DETAILED DESCRIPTION

[0019] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the accompanying drawings. The embodiments mentioned are all implemented on the premise of the technical solutions of the present invention, and detailed implementation processes are given, but it should be stated that the protection scope of the present invention is not limited to the following embodiments.

[0020] The present invention provides ( E )-2-hexenal is used as a rice anti-striped stem borer inducer, wherein the rice anti-striped stem borer inducer contains ( E )-2-hexenal. E The various uses of )-2-hexenal to induce defense and alertness in rice plants belong to the technical solutions to be protected by the present invention. The present invention is further explained below through examples and effect measurements.

[0021] Example 1 ( E The invention discloses a use of )-2-hexenal as a rice anti-striped stem borer inducer, which includes a method for preparing the inducer, releasing the inducer and inducing rice defense alert.

[0022] Preparation of inducer: E )-2-hexenal as the effective component, dichloromethane as the solvent, and prepared into ( E )-2-hexenal solution with a concentration of 15 μg / μL was used as an inducer for rice resistance to Chilo suppressalis. E )-2-Hexenal is a commercial reagent, and the product information is: purity>98%, Sigma Aldrich brand.

[0023] Preparation of volatile dispenser: 100 mg of glass wool (Sigma Aldrich) was placed in a 2 mL HPLC glass vial (Agilent), washed with dichloromethane, dried and cooled to room temperature, and then 0.4 mL of inducer was added. The vial was sealed with a cap with a rubber septum, and a 2 μL capillary (Drummond) was passed through the rubber septum, such as Figure 1 Then wrap the vial with aluminum foil to isolate it from light and heat and prevent the compound from decomposing.

[0024] Inducer induction experiment: The prepared inducer release device was hung in a glass bottle, and after stabilization at room temperature for 24 hours, it was connected to the ventilation system. The release device loaded with only dichloromethane was used as a control. Before the experiment, the rice plants were tightly wrapped with tin foil to prevent the soil and plastic pots from emitting gases that affected the experiment. After the air was filtered through activated carbon, fuchsin silica gel, and molecular sieves, it was connected to an airflow meter to control the flow rate at 600 mL / min, and then connected to the glass bottle with or without the inducer release device. The different volatile gases finally flowed out through the glass bottle containing the rice plants.

[0025] Test and analysis of the effect of induced rice defense By inducing the release device ( E )-2-hexenal release rate was tested, and bioassays of Chilo suppressalis larvae, and determination of jasmonic acid and trypsin inhibitor content in rice samples were conducted to determine ( E )-2-hexenal as the active component of rice resistance to Chilo suppressalis inducer has a significant effect in inducing rice defense alert.

[0026] Comparison of the growth and development of Chilo suppressalis larvae on rice induced with and without inducers By collecting and analyzing the release rates of inducers containing different concentrations of (E)-2-hexenal in the release device, the results show that the release rate of (E)-2-hexenal increases with the increase of its compound concentration; from the trend point of view, after the concentration of (E)-2-hexenal reaches 15 μg / μL in Example 1, its release rate tends to be flat as the concentration increases, and the release rate tends to be stable, such as Figure 2 As shown. According to the collection of the release device and GC-MS analysis, when the concentration of (E)-2-hexenal was 15 μg / μL and the airflow meter flow rate was 600 mL / min, the release amount of the release device was close to the content of volatiles released by rice induced by Chilo suppressalis for five days, as shown in Figure 3 As shown, SSB-5d represents rice induced by Chilo suppressalis for five days, and Dispenser represents the releasing device.

[0027] To further identify E )-2-hexenal as the function of the effective component of the inducer, the present invention conducted a bioassay experiment on the larvae of the striped stem borer for Example 1. After 24 hours of induction with or without the inducer (the exposure method is the same as in Example 1), three 3-day-old striped stem borer larvae were inoculated into each rice plant, and after feeding for 5 days, they were taken out and weighed. The average weight of the striped stem borer larvae on each rice plant was taken as a sample, and 16 biological replicates were used for each treatment. The results showed that loading ( E The weight of the larvae of Chilo suppressalis on rice induced by )-2-hexenal was significantly lower than that of the control group (exposed to the solvent dichloromethane) ( P = 0.038), such as Figure 4 As shown. Exposure to the inducer component ( E )-2-hexenal can enhance the resistance of rice to Chilo suppressalis.

[0028] Changes of phytohormone jasmonic acid content in rice tissues treated with or without Chilo suppressalis inducer The content of the plant hormone jasmonic acid in the rice samples treated with or without the Chilo suppressalis inducer in Example 1 was measured. Figure 5The results showed that exposure to the inducer ( E )-2-hexenal could not directly induce an increase in jasmonic acid content in rice, but the accumulation of jasmonic acid in rice plant tissues increased significantly after exposure to the inducer and exposure to the larvae of the stem borer for 6 h ( P = 0.005).

[0029] Changes of trypsin inhibitor content in rice tissues treated with or without Chilo suppressalis inducer The trypsin inhibitor (PI) content in the rice samples treated with or without the Chilo suppressalis inducer in Example 1 was determined. Figure 6 The results showed that exposure to the inducer ( E )-2-hexenal could not directly induce the increase of PI content in rice; however, after the rice exposed to the inducer was infested by the larvae of the stem borer for 12 h, the PI content in the rice plant tissue increased significantly ( P = 0.0048).

[0030] In summary, when ( E When )-2-hexenal was used as the active component of the rice anti-striped stem borer inducer, the growth and development of the larvae of the stem borer feeding on rice exposed to the inducer was significantly reduced, which fully demonstrated that exposure to ( E )-2-hexenal inducer can significantly enhance the resistance of rice to Chilo suppressalis. E After the rice planted with the )-2-hexenal inducer was attacked by the Chilo suppressalis, the content of plant hormone jasmonic acid and trypsin inhibitor in its tissues increased rapidly, enhancing the direct resistance of rice to the Chilo suppressalis. Therefore, before the arrival of the Chilo suppressalis, E The release device of )-2-hexenal solution is placed in the rice field, which can induce the rice plants to be on guard and reduce the cost loss. The preparation method of the inducer is simple, the use is convenient and fast, and it is green and safe. Wide promotion can reduce the use of chemical pesticides and has broad economic, social and ecological benefits.

[0031] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. (E) -2-hexenal is used as a rice anti-striped stem borer inducer, characterized in that: The effective component of the rice anti-striped stem borer inducer contains (E) -2-Hexenal.

2. The use according to claim 1, characterized in that The effective components of the rice anti-striped stem borer inducer are (E) -2-Hexenal single component.

3. The use according to claim 2, characterized in that: The rice resistance to Chilo suppressalis inducer is a solution containing the effective component, and the solvent of the solution is a volatile solvent.

4. The use according to claim 3, characterized in that The volatile solvent is selected from any one of dichloromethane, n-hexane and acetone.

5. The use according to claim 3, characterized in that: The solution (E) -The molar ratio of 2-hexenal to solvent is 1.5~150μg / μL.

6. The use according to claim 3, characterized in that: The solution (E) -The amount ratio of 2-hexenal to the solvent is 10-30 μg / μL.

7. The use according to any one of claims 1 to 6, characterized in that: The rice anti-striped stem borer inducer is loaded on a carrier to make a carrier capable of releasing (E) -2-hexenal release device, the carrier is a container or an adsorbent.

8. The use according to claim 7, characterized in that The release device is placed in a rice field, and the release device is used to release the rice (E) Volatiles of 2-hexenal induce defense alert in rice.

9. The rice resistance to Chilo suppressalis inducer for use according to any one of claims 1 to 6.

10. The rice resistance to Chilo suppressalis inducing agent according to claim 9, characterized in that: The inducer may also include any one or more of a surfactant, an adhesive, a dispersant, a propellant, a stabilizer, a pesticide, an insect repellent or a synergist.

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