Traditional Chinese medicine composition for preventing or treating frog tilting disease

Abstract

The invention belongs to the field of aquaculture, and particularly relates to a traditional Chinese medicine composition for preventing or treating frog tilting disease, which comprises gallnut, myrobalan and sappan wood. By using Chinese herbal medicines, the death rate of frogs is reduced, bacterial drug resistance is prevented, and green and healthy development of the rice frog industry is promoted.

Description

Technical Field

[0001] The invention belongs to the field of aquaculture, and particularly relates to a traditional Chinese medicine composition for preventing or treating frog head crookedness. Background Art

[0002] Rice-frog farming is a model of integrated rice-fish farming. With the continuous expansion of rice-frog farming and the deterioration of the farming environment, bacterial diseases of black-spotted frogs have seriously restricted the healthy development of rice-frog farming. In 1993, the "head crooked disease" of bullfrogs was reported in China. After decades of farming, the pathogen has gradually evolved from infecting bullfrogs to infecting other types of black-spotted frogs. In 2013, the "head crooked disease" was first reported on artificially cultivated black-spotted frogs (commonly known as black-spotted frogs). After 4 years of research, it was finally determined that the pathogen was Elizabeth Milbek. Up to now, the types of pathogens of the "head crooked disease" of black-spotted frogs are even more diverse. In 2023, we simultaneously isolated Elizabeth Milbek and Klebsiella pneumoniae from black-spotted frogs with symptoms of "head crooked disease". These bacteria are highly pathogenic, widely distributed, and have a wide spectrum of drug resistance. If antibiotics are used alone for treatment, it will not only lead to bacterial resistance, but also make black-spotted frog farming more and more difficult.

[0003] The typical symptoms of the black-spotted frog's "head crooked disease" are head crookedness and cataracts in the eyes. Since bacteria exist in tissues such as the brain, kidneys and liver, and the brain has a blood-brain barrier, general antibiotics have a poor ability to pass through the blood-brain barrier, so conventional antibiotics are very ineffective in treating the black-spotted frog's "head crooked disease". As the material basis of traditional Chinese medicine, Chinese herbal medicine is rich in a variety of natural active ingredients that can improve the body's physical fitness and immunity, and can also inhibit bacteria and kill bacteria. Therefore, it is necessary to develop a Chinese herbal medicine formula for the prevention and treatment of the black-spotted frog's "head crooked disease", while reducing the use of antibiotics while preventing and treating, and promoting the green and healthy development of the rice frog industry. Summary of the invention

[0004] In order to achieve the above-mentioned purpose, the technical solution adopted by the present invention is to develop a Chinese medicine composition for preventing or treating frog head crookedness, and to study the addition amount of the Chinese medicine composition in feed. By using Chinese herbal medicines, while reducing frog mortality, it prevents the development of bacterial resistance and promotes the green and healthy development of the rice frog industry.

[0005] In order to solve the above technical problems, the following technical solutions are adopted:

[0006] A traditional Chinese medicine composition for preventing or treating frog head tilt disease comprises: gallnut, terminalia chebula and sappan wood.

[0007] The above-mentioned Chinese medicinal composition for preventing or treating frog crooked head disease comprises 60-90 parts of gallnut, 1-30 parts of terminalia chebula and 5-30 parts of sappan wood based on 100 parts of total weight.

[0008] The above-mentioned traditional Chinese medicine composition for preventing or treating frog crooked head disease comprises 70-90 parts of gallnut, 5-20 parts of terminalia chebula and 5-20 parts of sappan wood based on 100 parts of total weight.

[0009] The above-mentioned traditional Chinese medicine composition for preventing or treating frog head tilt disease, the frog is black-spotted frog or bullfrog.

[0010] The above-mentioned traditional Chinese medicine composition for preventing or treating frog head tilt disease is a composition formed by mixing the raw materials after crushing, or an extract obtained by mixing or extracting the raw materials separately, or an effective part obtained by further refining and purifying the extract, or a conventional dosage form made by adding pharmaceutically acceptable excipients.

[0011] The above-mentioned traditional Chinese medicine composition for preventing or treating frog head tilt disease is a composition formed by crushing and mixing various raw materials.

[0012] On the other hand, the present invention provides the use of the above-mentioned traditional Chinese medicine composition in the preparation of medicines, foods or health products for preventing or treating frog wry head disease.

[0013] The above application is characterized in that the medicine, food or health product comprises the above traditional Chinese medicine composition and polymyxin B.

[0014] In another aspect, the present invention provides a method for preparing a Chinese herbal medicine feed for preventing or treating frog head tilt disease, comprising:

[0015] (1) Grind Chinese gallnut, myrobalan, and Sappan wood into powder respectively for later use;

[0016] (2) dissolving the gallnut powder and polymyxin B in water at 40-70° C. according to a ratio, and then adding the chebula powder and the sappan wood powder according to a ratio to obtain a Chinese herbal medicine solution;

[0017] (3) Evenly mix the Chinese herbal medicine liquid with the feed, and dry naturally to obtain the Chinese herbal medicine feed. The preparation method of the Chinese herbal medicine feed for preventing or treating frog head crookedness, in parts by weight, comprises: 100 parts of feed, 1.5-2.0 parts of the Chinese medicine composition, and 0.02-0.04 parts of polymyxin B. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Various other advantages and benefits will become apparent to those of ordinary skill in the art by reading the following detailed description of the preferred embodiment.The drawings are only for the purpose of illustrating the preferred embodiments and are not to be construed as limiting the invention.

[0019] Figure 1 is the diameter of the inhibition zone of different Chinese herbal medicine formulations;

[0020] Figure 2 is the cumulative mortality rate after black-spotted frogs were infected with pathogenic bacteria at different concentrations;

[0021] Figure 3 It is a black-spotted frog infected with pathogenic bacteria;

[0022] Figure 4 The cumulative mortality of black-spotted frogs infected with pathogenic bacteria and fed with Chinese herbal medicines mixed with different concentrations;

[0023] Figure 5 The cumulative mortality rate of black-spotted frogs injected with pathogenic bacteria and fed with 15‰ of Chinese herbal medicine mixed feed. DETAILED DESCRIPTION

[0024] In order to fully understand the purpose, features and effects of the present invention, the present invention is described in detail through the following specific embodiments. Except for the following contents, the process method of the present invention adopts conventional methods or devices in the art. Unless otherwise specified, the following terms have the meanings commonly understood by those skilled in the art.

[0025] When a numerical range is disclosed herein, the above range is considered to be continuous and includes the minimum and maximum values ​​of the range, as well as every value between such minimum and maximum values. Further, when a range refers to an integer, every integer between the minimum and maximum values ​​of the range is included. In addition, when multiple ranges are provided to describe a feature or characteristic, the ranges can be combined. In other words, unless otherwise indicated, all ranges disclosed herein should be understood to include any and all subranges included therein.

[0026] Specifically, the Chinese medicinal composition for preventing or treating frog wry head disease provided by the present invention comprises: Galla chinensis, Terminalia chebula and Sappan wood.

[0027] The Chinese medicine composition of the present invention has antibacterial effects through the gallnut tannic acid, the tannin extract of myrobalan and the hematoxylin of sappan wood. The three can reduce the mortality rate of frogs through synergistic effect, prevent the occurrence of bacterial resistance, and promote the green and healthy development of the rice frog industry.

[0028] The Chinese medicine composition of the present invention can be used to prevent or treat head crookedness of black-spotted frog or bullfrog.

[0029] In some preferred embodiments, based on 100 parts by total weight, the traditional Chinese medicine composition includes: 60-90 parts of Galla chinensis, 1-30 parts of Terminalia chebula, and 5-30 parts of Sappan wood.

[0030] When the ratio of the three Chinese medicinal ingredients is too large or too small, the effect of preventing or treating black-spotted frog or bullfrog head crooked disease will be reduced.

[0031] Further preferably, based on 100 parts by total weight, the Chinese medicine composition comprises: 70-90 parts of Galla chinensis, 5-20 parts of Terminalia chebula, and 5-20 parts of Sappan wood.

[0032] Specifically, in one embodiment, the Chinese medicine composition of the present invention comprises: 80 parts of gallnut, 10 parts of terminalia chebula, and 10 parts of sappan wood; in another embodiment, the Chinese medicine composition of the present invention comprises: 70 parts of gallnut, 20 parts of terminalia chebula, and 10 parts of sappan wood.

[0033] Among them, the Chinese medicine composition is a composition formed by mixing the raw materials after crushing, or an extract obtained by mixing or extracting the raw materials separately, or an effective part obtained by further refining and purifying the extract, or a conventional dosage form made by adding pharmaceutically acceptable excipients.

[0034] Among them, the extraction methods include decoction extraction, reflux extraction, immersion extraction, ultrasonic extraction, percolation extraction, and microwave extraction; the extraction solvents include water or conventional organic solvents, such as ethanol, methanol, ethyl acetate, petroleum ether, isopropanol, etc.; the refining and purification processes include extraction, column chromatography separation, high performance liquid chromatography separation, etc.

[0035] In some preferred embodiments, the traditional Chinese medicine composition is a composition obtained by mixing various raw materials after crushing.

[0036] On the other hand, the present invention also provides the use of the traditional Chinese medicine composition in the preparation of medicines, foods or health products for preventing or treating frog wry head disease.

[0037] In some preferred embodiments, the medicine, food or health product comprises the above-mentioned traditional Chinese medicine composition and polymyxin b.

[0038] As a polypeptide antibiotic, polymyxin B has no antagonistic effect with the Chinese herbal medicine. A small amount of polymyxin B can quickly reach other tissues and organs except the brain through the blood, quickly inhibit the pathogenic bacteria of these organs, improve the physical fitness of black-spotted frogs, and restore their full feeding ability, laying the foundation for subsequent Chinese herbal medicines to reach the brain to exert antibacterial and bactericidal effects.

[0039] In another aspect, the present invention further provides a method for preparing a Chinese herbal medicine feed for preventing or treating frog head tilt disease, characterized in that it comprises:

[0040] (1) Grind Chinese gallnut, myrobalan, and Sappan wood into powder respectively for later use;

[0041] (2) dissolving the gallnut powder and polymyxin B in water at 40-70° C. according to the ratio, and then adding the chebula powder and the sappan wood powder to obtain a Chinese herbal medicine solution;

[0042] (3) The Chinese herbal medicine liquid and the feed are mixed evenly, and the mixture is naturally dried to obtain the Chinese herbal medicine feed.

[0043] The ratio of gallnut, terminalia chebula and sappan wood is the same as that in the traditional Chinese medicine composition provided in the first aspect of the present invention, and the present invention will not repeat it here.

[0044] In some preferred embodiments, the Chinese herbal medicine feed comprises, by weight: 100 parts of feed, 1.5-2.0 parts of Chinese medicine composition, and 0.02-0.04 parts of polymyxin B.

[0045] When the Chinese herbal medicine composition in the herbal feed is too much, the cost of use is too high and the control effect is not significantly improved; when the Chinese herbal medicine composition is too little, the control effect is reduced. When the proportion of polymyxin B is too much, it may lead to antibiotic resistance; when the proportion of polymyxin B is too little, the control effect may take longer.

[0046] According to practice, the herbal feed for treating black-spotted frog with head crookedness is fed once in the morning and once in the evening, and the continuous feeding time is not less than 7 days. This can significantly reduce the mortality rate of black-spotted frogs.

[0047] Example

[0048] The present invention is further described below by way of examples, but the present invention is not limited to the scope of the examples. The experimental methods in the following examples without specifying specific conditions are carried out according to conventional methods and conditions. The raw materials used in the following examples are all conventionally purchased.

[0049] Experiment 1: Selection of Chinese herbal medicines

[0050] Elizabethella miltiorrhiza and Klebsiella pneumoniae were isolated from the tissues of black-spotted frogs with "head crooked disease" and confirmed as the pathogens of "head crooked disease". On this basis, a variety of commercially available Chinese herbal medicines were used for drug sensitivity tests. The Chinese herbal medicines were prepared into a concentration of 1000 mg / mL using the 96-well microbroth two-fold dilution method and diluted in multiple proportions to add to a 1.0×10 7Incubate in the micro-wells of CFU / mL Elizabethkingia miricola and Klebsiella pneumoniae. The dilution wells of the experimental group contain broth medium, pathogenic bacteria, and Chinese herbal medicines at different concentrations, while the negative control group only contains broth medium. The concentrations of Chinese herbal medicines in 10 serial dilution wells are 1000, 500, 250, 125, 62.5, 31.25, 15.625, 7.81, 3.9, and 1.95 mg / mL respectively. The group with similar OD values between the experimental group and the negative control group is the minimum inhibitory concentration (MIC) of the drug to be tested. The larger the MIC value, the worse the bactericidal effect on pathogenic bacteria, and vice versa. At the same time, the MIC tests of antibiotics are also carried out. Specifically, ciprofloxacin and polymyxin b are respectively prepared into a concentration of 320 μg / mL and serially diluted and added to the micro-wells containing 1.0×10 7 Incubate in the micro-wells of CFU / mL Elizabethkingia miricola and Klebsiella pneumoniae. The dilution wells of the experimental group contain broth medium, pathogenic bacteria, and antibiotics at different concentrations, while the negative control group only contains broth medium. The concentrations of antibiotics in 10 serial dilution wells are 320, 160, 80, 40, 20, 10, 5, 2.5, 1.25, and 0.625 μg / mL respectively. Finally, the MICs of ciprofloxacin and polymyxin b against Elizabethkingia miricola are 10 and 10 μg / mL respectively; the MICs of ciprofloxacin and polymyxin b against Klebsiella pneumoniae are 10 and 5 μg / mL respectively. The growth conditions of bacteria in each Chinese herbal medicine are shown in Table 1:

[0051] Table 1

[0052]

[0053] Based on the above data, combined with MIC, the price of Chinese herbal medicines, and commerciality, we selected Chinese gallnut, smoked plum, Tibetan medicine terminalia fruit, and sappanwood for further research.

[0054] Experiment 2: Determination of fractional inhibitory concentration (FIC)

[0055] Since there are antagonistic or synergistic effects among Chinese herbal medicines, when multiple Chinese herbal medicines are used in combination, it is necessary to determine the FIC values between them. The FIC (Fractional Inhibitory Concentration) values of the combination of 4 Chinese herbal medicines and 2 antibiotics are determined by the micro-broth checkerboard method. The calculation formula of FIC is: (MIC of drug A in combination / MIC of drug A alone) + (MIC of drug B in combination / MIC of drug B alone). The judgment criteria are as follows: if FIC ≤ 0.5, then drug A and drug B have a synergistic effect; if 0.5 < FIC ≤ 1, then drug A and drug B have an additive effect; if 1 < FIC ≤ 1.5, then drug A and drug B have no relevant effect; if FIC ≥ 2, then drug A and drug B have an antagonistic effect. The FIC values of Chinese herbal medicines are shown in Table 2.

[0056] Table 2

[0057] Gallnut Black plum Terminalia chebula Sappan Ciprofloxacin Polymyxin B Gallnut 1.25 1.25 0.5625 1.25 1.06 Black plum 1.25 1.5 1.06 >2 >2 Terminalia chebula 1.25 1.5 0.5625 >2 1.5 Sappan 0.5625 1.06 0.5625 1.5 0.375 Ciprofloxacin 1.25 >2 >2 1.5 - Polymyxin B 1.06 >2 1.5 0.375 -

[0058] Note: The symbol “-” in the table means that the FIC between antibiotics has not been determined; a blank table means that a single drug has no FIC by itself.

[0059] According to the above data, due to the antagonism between black plum and antibiotics, ciprofloxacin has antagonistic effects on black plum and golden terminalia, so black plum and ciprofloxacin were finally removed. Only three Chinese herbal medicines, gallnut, golden terminalia and sappan wood, were selected for combined use.

[0060] Test 3: In vitro antibacterial test of Chinese herbal medicine formula

[0061] After selecting the above three Chinese herbal medicines, it is necessary to study the ratio between the three to find the optimal ratio combination to ensure the best efficacy. According to the weight calculation, the gallnut, the golden terminalia and the sappan wood are respectively prepared into a mixture in the ratio of 9.5:0.4:0.1, 9.5:0.1:0.4, 9:0.9:0.1, etc., and then the composite Chinese herbal medicine liquid is prepared by water decoction. After the liquid is sterilized, it is stored at 4°C for use, and the final concentration of the liquid is 1g / mL.

[0062] Prepare BHI agar culture plates, add 200 μL of the reserved bacterial solution on each plate, spread it evenly with a sterile glass rod, and use sterile tweezers to stick the paper strips soaked with the compound Chinese herbal medicine on the surface of the BHI agar culture plate. Repeat three times for each drug-sensitive paper strip. After culturing in a 37°C incubator for 24 hours, observe the formation of the inhibition zone in the BHI agar culture plate and measure the diameter of the inhibition zone with a vernier caliper. The larger the diameter of the inhibition zone, the more obvious the in vitro antibacterial effect of the compound Chinese herbal medicine. The results are as follows Figure 1 As shown:

[0063] according to Figure 1 The results show that when the proportion of Galla chinensis is within the range of 60-90%, the proportion of Terminalia chebula is 1-30%, and the proportion of Sappan wood is 5-30%, the compound Chinese herbal medicine has the largest inhibition zone against Elizabeth Millipore alone, Klebsiella pneumoniae alone, and a mixture of the two.

[0064] Test 4: Concentration test of infection with pathogens of head tilt disease

[0065] Healthy and disease-free black-spotted frogs weighing about 50g were selected and divided into 9 groups, with 30 in each group. Then, Elizabeth Milbemilla alone, Klebsiella pneumoniae alone, and a mixture of the two were prepared at concentrations of 10^4, 10^5, and 10^6 CFU / mL to soak the black-spotted frogs for 1 hour to infect them with bacteria. The control group was not soaked. The soaked black-spotted frogs were temporarily kept in a special cement pool. Feed was fed normally every day and the cement pool was cleaned. The number of deaths was counted every day, and the cumulative mortality rate over 7 days was calculated. Experimental process and results Figure 2 As shown:

[0066] according to Figure 2 It can be seen that the 7-day cumulative mortality rate of the group infected with 10^6 CFU / mL of Elizabeth Millipore alone reached more than 35%, and the 7-day cumulative mortality rate of the group infected with 10^6 CFU / mL of Klebsiella pneumoniae alone and mixed bacteria was the highest, at around 50%. The control group did not have bacterial infection and no deaths occurred during the experiment. Based on this, subsequent experiments selected 10^6 CFU / mL of mixed bacteria to carry out Chinese herbal medicine prevention and treatment experiments, and according to Figure 2 It can be seen that the mortality rate of all experimental groups infected for 1 hour in the first 3 days was relatively low. Therefore, the time of subsequent immersion experiments was extended to 3 hours to ensure that the black-spotted frogs were fully exposed to the bacteria and infected.

[0067] We infected the black-spotted frog ( Figure 3 ) were analyzed, and the black-spotted frog showed obvious head tilt and eye cataracts, indicating that the black-spotted frog died of head tilt disease. After dissecting the dead frogs, we isolated Elizabeth Milbe and Klebsiella pneumoniae from the eyes, brain, liver and kidneys, further indicating that the pathogens of the black-spotted frog head tilt disease are Elizabeth Milbe and Klebsiella pneumoniae.

[0068] Experiment 5: Chronic in vivo antibacterial test of Chinese herbal medicine formula on black-spotted frog

[0069] Healthy and disease-free black-spotted frogs weighing about 50g were selected and divided into 4 groups, each with 30 frogs. Then, 10^6CFU / mL of Elizabeth Millard / Klebsiella pneumoniae mixed bacteria were used to soak the black-spotted frogs for 3 hours to infect them with bacteria. The soaked black-spotted frogs were temporarily kept in a special cement pool, and then mixed with compound Chinese herbal medicines (the compound Chinese herbal medicine ratio was 70 parts of Galla chinensis, 20 parts of Terminalia chebula, and 10 parts of Sappan wood). The amount of mixed medicines accounted for 3‰, 15‰ and 30‰ of the weight of the feed. Each group was also added with 0.2‰ of polymyxin B by weight of the feed, and the control group was not mixed with medicine. Normal feeding and cleaning of the cement pool were carried out every day. The number of deaths was counted every day, and the cumulative mortality rate was calculated. The test results are as follows Figure 4 .

[0070] according to Figure 4It can be seen that the cumulative mortality rate of the 3‰ mixed medicine feeding group is equivalent to that of the control group, and it tends to stabilize in about 7 days. The cumulative mortality rate of both is above 40%. The cumulative mortality rate of the 15‰ mixed medicine feeding group is the lowest, only about 20%; the cumulative mortality rate of the 30‰ mixed medicine feeding group is second, at about 27%. The survival rate of the 15‰ mixed medicine feeding group increased by 20% compared with the control group, and the effect was significant. Based on this, it is determined that the optimal addition of Chinese herbal compound in feed is ≥15‰ but ≤20‰.

[0071] Experiment 6: In vivo acute antibacterial test of herbal formula on Rana nigromaculata

[0072] Healthy and disease-free black-spotted frogs weighing about 50g were selected and divided into two groups, each with 30 frogs. Then, 10^9CFU / mL of Elizabeth Millipore / Klebsiella pneumoniae mixed bacteria were used to inject the black-spotted frogs. Each frog was injected with 200uL. The injected black-spotted frogs were temporarily kept in a special cement pool, and then mixed with compound Chinese herbal medicines (the compound Chinese herbal medicine ratio was 80 parts of Galla chinensis, 10 parts of Terminalia chebula, and 10 parts of Sappan wood). The amount of mixed medicines accounted for 15‰ of the weight of the feed. In addition, 0.2‰ of the weight of polymyxin B was added to the feed. The control group was not mixed with medicines. Normal feeding was performed every day and the cement pool was cleaned. The number of deaths was counted every day, and the cumulative mortality rate was calculated. The test results are as follows Figure 5 .

[0073] according to Figure 5 It can be seen that after the injection of pathogenic bacteria, the cumulative mortality rate of the control group reached 40%, while the mortality rate of the experimental group was stabilized at around 18% due to the addition of Chinese herbal medicine mixed with feed, and the survival rate was increased by about 22% compared with the control group.

[0074] Experiment 7: In vivo antibacterial test of Chinese herbal medicine formula on bullfrog

[0075] Since head wriggling disease originally originated from bullfrogs and Elizabeth Milbe was also isolated from bullfrogs, we also conducted preliminary research on the preventive and therapeutic effects of Chinese herbal medicine formulas on bullfrog head wriggling disease.

[0076] We selected a bullfrog pond with crooked head disease and first used 30‰ mixed medicine (the ratio of compound Chinese herbal medicine is 80 parts of Galla chinensis, 10 parts of Chebula chinensis, and 10 parts of Sappan wood) to feed for 2 weeks, and then used 15‰ mixed medicine to feed for 2 weeks, cleaning up dead bullfrogs every day and keeping records. According to the data of Experiment 4 and Experiment 5, it can be seen that the mortality rate can only be stabilized after about 7 days of infection, and the efficacy of the mixed medicine can only be stabilized after about 7 days of feeding. Therefore, the results of the 30‰ and 15‰ mixed medicine tests are the cumulative number of deaths on the 7th to 14th day of feeding. The test process and results are shown in Table 3:

[0077] Table 3

[0078]

[0079]

[0080] According to the above data, the cumulative mortality rate of bullfrogs fed with 30‰ Chinese herbal medicine compound was 38 in 7 days, which was 57.8% lower than the mortality rate of 90 in the control group, and the control effect was very obvious. The cumulative mortality rate of bullfrogs fed with 15‰ Chinese herbal medicine compound was only 9 in 7 days, which was 10% of the mortality rate of the control group and 23.7% of the mortality rate of the 30‰ compound group, which was 90% lower than the mortality rate of the control group, and the control effect was extremely significant.

[0081] The present invention has been disclosed above with preferred embodiments, but those skilled in the art should understand that these embodiments are only used to describe the present invention and should not be understood as limiting the scope of the present invention. It should be noted that all changes and substitutions equivalent to these embodiments should be deemed to be included in the scope of the claims of the present invention. Therefore, the scope of protection of the present invention should be based on the scope defined in the claims.

Claims

1. A Chinese medicine composition for preventing or treating frog head tilt disease, characterized in that: include: Gallnut, Terminalia chebula, and Sappan wood.

2. The Chinese medicine composition according to claim 1, characterized in that: The total weight of the invention is 100 parts, and the invention comprises 60-90 parts of gallnut, 1-30 parts of terminalia chebula and 5-30 parts of sappan wood.

3. The Chinese medicine composition according to claim 1, characterized in that Based on 100 parts by total weight, the traditional Chinese medicine composition comprises: 70-90 parts of gallnut, 5-20 parts of terminalia chebula, and 5-20 parts of sappan wood.

4. The Chinese medicine composition according to claim 1, characterized in that: The frog is a black-spotted frog or a bullfrog.

5. The Chinese medicine composition according to claim 1, characterized in that: The Chinese medicine composition is a composition obtained by mixing the raw materials after crushing, or an extract obtained by mixing or extracting the raw materials separately, or an effective part obtained by further refining and purifying the extract, or a conventional dosage form prepared by adding pharmaceutically acceptable excipients.

6. The Chinese medicine composition according to claim 1, characterized in that: The Chinese medicine composition is a composition obtained by mixing various raw materials after being crushed.

7. Use of the Chinese medicine composition according to any one of claims 1 to 5 in the preparation of medicines, foods or health products for preventing or treating frog wry head disease.

8. The use according to claim 7, characterized in that: The medicine, food or health product comprises the traditional Chinese medicine composition according to any one of claims 1 to 5 and polymyxin B.

9. A method for preparing a Chinese herbal medicine feed for preventing or treating frog head tilt disease, characterized in that: include: (1) Grind Chinese gallnut, myrobalan, and Sappan wood into powder respectively for later use; (2) dissolving the gallnut powder and polymyxin B in water at 40-70° C. according to a ratio, and then adding the chebula powder and the sappan wood powder according to a ratio to obtain a Chinese herbal medicine solution; (3) The Chinese herbal medicine liquid and the feed are mixed evenly, and the mixture is naturally dried to obtain the Chinese herbal medicine feed.

10. The preparation method according to claim 9, wherein the Chinese herbal medicine feed comprises, by weight: 100 parts of feed, 1.5-2.0 parts of Chinese medicine composition, and 0.02-0.04 parts of polymyxin B.

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