Medicament for preventing and treating damping off and preparation method thereof

Abstract

The invention discloses a damping-off prevention and treatment agent and a preparation method thereof. The damping-off prevention and treatment agent is prepared from the following raw materials: a composite antibacterial agent, a composite plant immunity inducer, a plant growth regulator, a composite surfactant and the like. According to the prevention and treatment agent provided by the invention, thiamethoxam, azoxystrobin and ipconazole are compounded to serve as a composite antibacterial agent, and under the condition of a small compounding amount, better insecticidal and antibacterial effects can be achieved; the compound plant inducer is added, so that the antioxidant enzyme and defensive enzyme activity of the plant root system is effectively enhanced, and the inhibition on the growth of the seedling root system is relieved, thereby promoting the growth of the seedling root system and enhancing the resistance of rice to damping off; nutrient substances needed by plant growth are supplemented from the plant growth regulator, and plant growth is promoted. According to the prevention and treatment medicament disclosed by the invention, the components are compounded from a plurality of aspects of sterilization and bacterium resistance, enhancement of the resistance of plants to damping off, nutrition supplement and growth promotion, so that the remarkable prevention and treatment and growth promotion effects are achieved.

Description

Technical Field

[0001] The invention relates to the field of pesticide preparation, and in particular to a pesticide for preventing and treating damping-off disease and a preparation method thereof. Background Art

[0002] Rice damping-off is a soil-borne disease caused by fungi such as Fusarium oxysporum. It is also the main disease of rice seedlings grown in dryland. After the disease occurs, it will cause the root of the seedlings to rot, resulting in the obstruction of nutrient absorption, the heart leaves to turn yellow, and the seedlings to dry up and die in large areas, which brings great harm to rice production. At present, fungicides are mainly used in production to prevent and control rice damping-off, but fungicides have many problems such as short duration of efficacy, easy to produce drug resistance, and environmental pollution. In the past, the main treatment for rice damping-off was the application of fungicides, which was cumbersome, polluted the environment, and had unsatisfactory effects. Pathogens, low temperature and temperature changes are necessary factors leading to the occurrence of rice damping-off.

[0003] Another important way to prevent and control plant diseases is to use plant immune inducers to induce the plant to produce disease resistance. Because of its long-lasting effect, broad spectrum, no resistance, low concentration, harmlessness to humans, and no pollution to the environment, it has been widely used in the prevention and control of plant diseases in recent years and has become a hot spot in plant disease prevention and control research. However, there are few reports on the technology of using inducers to prevent and control rice damping-off disease. Research on plant immune inducers to induce rice resistance to damping-off disease is of great significance to the establishment of a green rice disease prevention and control and pesticide reduction technology system.

[0004] The combined effect of plant growth regulators and fungicides can play a certain role in antibacterial and growth promotion. However, in the prior art, fungicides and related plant fertilizers are generally applied simultaneously without further research on the compatibility and synergy of the overall compounding of ingredients, which often leads to unsatisfactory antibacterial effects and poor utilization of agents. Moreover, the prior art lacks an agent that can significantly kill bacteria and promote plant growth while reducing the addition of bactericidal active ingredients. Summary of the invention

[0005] Based on this, in order to solve the problems of single function, unsatisfactory bactericidal effect and poor utilization rate of the existing control agents, the present invention provides a control agent for damping-off disease and a preparation method thereof, and the specific technical scheme is as follows:

[0006] A damping-off control agent, comprising the following raw materials in parts by weight: 9 to 17 parts of a composite antibacterial agent, 7 to 15 parts of a composite plant immunity inducer, 1 to 3 parts of a plant growth regulator, 1 to 3 parts of an emulsifier, 2 to 5 parts of a composite surfactant, 1 to 3 parts of a thickener, 1 to 3 parts of a dispersant, 1 to 3 parts of a preservative, and 45 to 80 parts of water;

[0007] Wherein, the composite antibacterial agent is obtained by mixing thiamethoxam, azoxystrobin and ipconazole;

[0008] The composite plant immune inducer is obtained by mixing chitosan oligosaccharide and benzothiadiazole;

[0009] The plant growth regulator is obtained by mixing potassium dihydrogen phosphate, ethephon and magnolol.

[0010] Furthermore, the mass ratio of thiamethoxam, myclobutanil and ipconazole is (1-5): (1-3): (1-5).

[0011] Furthermore, the mass ratio of the chitosan oligosaccharide to benzothiadiazole is (1-7): (1-2).

[0012] Furthermore, the mass ratio of potassium dihydrogen phosphate, ethephon and magnolol is (1-3): (1-2): (1-5).

[0013] Furthermore, the emulsifier is at least one of trialkylphenol polyoxyethylene ether, phenethylphenol polyoxyethylene ether and fatty acid methyl ester polyether sulfonate.

[0014] Furthermore, the composite surfactant comprises trisiloxane and sodium fatty alcohol polyoxyethylene ether sulfate in a mass ratio of (1-3):(1-7).

[0015] Furthermore, the thickener is at least one of xanthan gum, magnesium aluminum silicate and sodium alginate.

[0016] Furthermore, the dispersant is at least one of sodium polynaphthalene sulfonate, sodium methylene bisnaphthalene sulfonate and sodium hexametaphosphate.

[0017] Furthermore, the preservative is at least one of sorbic acid, sodium benzoate and kason.

[0018] In addition, the present invention also provides a method for preparing a drug for preventing and treating damping-off disease, the method comprising the following steps:

[0019] The composite antibacterial agent, the composite plant immune inducer, the plant growth regulator, the emulsifier, the composite surfactant, the thickener, the dispersant, the preservative and water are mixed by weight, and stirred at a rotation speed of 2000 r / min to 15000 r / min for 20 min to 45 min to obtain a control agent for damping-off disease.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] 1. The control agent provided by the present invention uses the compounded composite antibacterial agent as an antibacterial active ingredient, so that a single component can play a better insecticidal and antibacterial effect in the case of a small compounding amount, especially has a significant killing effect on rice thrips; combined with the addition of a composite plant inducer, the antioxidant enzyme and defense enzyme activity of the plant root system are effectively enhanced, the growth of the seedling root system is alleviated, thereby promoting the growth of the seedling root system and enhancing the resistance of rice to damping-off; and the nutrients required for plant growth are supplemented from the plant growth regulator to further promote plant growth. That is, the control agent of the present invention is compounded from multiple angles of insecticidal and antibacterial-enhancing the resistance of the plant itself to damping-off-supplementing nutrition to promote growth, so as to achieve significant control and growth-promoting effects.

[0022] 2. The present invention optimizes the composition of the composite surfactant, which helps to improve the spreading and deposition of the control agent on the plant surface, increase the retention of the control agent on the plant surface, and thus improve the utilization rate of the control agent to a certain extent.

[0023] 3. The compound system of the control agent of the present invention has good compatibility and excellent stability, has a significant control effect on rice damping-off disease, and is suitable for actual production use. DETAILED DESCRIPTION

[0024] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with its embodiments. It should be understood that the specific implementation methods described herein are only used to explain the present invention and do not limit the protection scope of the present invention.

[0025] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0026] In one embodiment of the present invention, a damping-off control agent comprises the following raw materials in parts by weight: 9 to 17 parts of a composite antibacterial agent, 7 to 15 parts of a composite plant immune inducer, 1 to 3 parts of a plant growth regulator, 1 to 3 parts of an emulsifier, 2 to 5 parts of a composite surfactant, 1 to 3 parts of a thickener, 1 to 3 parts of a dispersant, 1 to 3 parts of a preservative, and 45 to 80 parts of water;

[0027] Wherein, the composite antibacterial agent is obtained by mixing thiamethoxam, azoxystrobin and ipconazole;

[0028] The composite plant immune inducer is obtained by mixing chitosan oligosaccharide and benzothiadiazole;

[0029] The plant growth regulator is obtained by mixing potassium dihydrogen phosphate, ethephon and magnolol.

[0030] In one implementation, the mass ratio of thiamethoxam, myclobutanil and ipconazole is (1-5): (1-3): (1-5).

[0031] In one implementation, the mass ratio of the chitosan oligosaccharide to benzothiadiazole is (1-7): (1-2).

[0032] In one implementation, the mass ratio of potassium dihydrogen phosphate, ethephon and magnolol is (1-3): (1-2): (1-5).

[0033] In one implementation, the emulsifier is at least one of trialkylphenol polyoxyethylene ether, phenethylphenol polyoxyethylene ether and fatty acid methyl ester polyether sulfonate.

[0034] In one implementation, the composite surfactant includes trisiloxane and sodium fatty alcohol polyoxyethylene ether sulfate in a mass ratio of (1-3):(1-7).

[0035] In one implementation, the thickener is at least one of xanthan gum, magnesium aluminum silicate and sodium alginate.

[0036] In one implementation, the dispersant is at least one of sodium polynaphthalene sulfonate, sodium methylene bisnaphthalene sulfonate and sodium hexametaphosphate.

[0037] In one implementation, the preservative is at least one of sorbic acid, sodium benzoate and kason.

[0038] In one embodiment, the present invention further provides a method for preparing a drug for preventing and controlling damping-off disease, the preparation method comprising the following steps:

[0039] The composite antibacterial agent, the composite plant immune inducer, the plant growth regulator, the emulsifier, the composite surfactant, the thickener, the dispersant, the preservative and water are mixed by weight, and stirred at a rotation speed of 2000 r / min to 15000 r / min for 20 min to 45 min to obtain a control agent for damping-off disease.

[0040] The control agents in the above scheme are compounded from multiple angles, including insecticide and antibacterial, enhancing the plant's own resistance to damping-off disease, and supplementing nutrition to promote growth, to achieve significant control and growth-promoting effects.

[0041] The embodiments of the present invention will be described in detail below with reference to specific examples.

[0042] Embodiment 1:

[0043] A method for preparing a damping-off disease control agent comprises the following steps:

[0044] By weight, 9 parts of a composite antibacterial agent (thiamethoxam, myclobutanil and chloranil in a mass ratio of 1:1:1), 12 parts of a composite plant immune inducer (chitosan oligosaccharide and benzothiadiazole in a mass ratio of 1:2), 3 parts of a plant growth regulator (potassium dihydrogen phosphate, ethephon and magnolol in a mass ratio of 1:1:1), 2 parts of trialkylphenol polyoxyethylene ether, 4 parts of a composite surfactant (trisiloxane and sodium fatty alcohol polyoxyethylene ether sulfate in a mass ratio of 1:1), 1 part of magnesium aluminum silicate, 3 parts of sodium hexametaphosphate, 3 parts of sodium benzoate and 55 parts of water were mixed, and the mixture was stirred at a speed of 5000 r / min for 45 min to obtain a control agent for damping-off disease.

[0045] Embodiment 2:

[0046] A method for preparing a damping-off disease control agent comprises the following steps:

[0047] By weight, 12 parts of a composite antibacterial agent (thiamethoxam, myclobutanil and ioconazole in a mass ratio of 1:2:3), 12 parts of a composite plant immune inducer (chitosan oligosaccharide and benzothiadiazole in a mass ratio of 2:1), 3 parts of a plant growth regulator (potassium dihydrogen phosphate, ethephon and magnolol in a mass ratio of 2:2:1), 2 parts of phenethyl polyoxyethylene ether, 4 parts of a composite surfactant (trisiloxane and sodium fatty alcohol polyoxyethylene ether sulfate in a mass ratio of 1:1), 3 parts of magnesium aluminum silicate, 2 parts of sodium polynaphthaldehyde sulfonate, 2 parts of sodium benzoate and 60 parts of water were mixed, and the mixture was stirred at a speed of 5000 r / min for 45 min to obtain a control agent for damping-off disease.

[0048] Embodiment 3:

[0049] A method for preparing a damping-off disease control agent comprises the following steps:

[0050] By weight, 16 parts of a composite antibacterial agent (thiamethoxam, myclobutanil and chloranil in a mass ratio of 3:1:4), 15 parts of a composite plant immune inducer (chitosan oligosaccharide and benzothiadiazole in a mass ratio of 4:1), 3 parts of a plant growth regulator (potassium dihydrogen phosphate, ethephon and magnolol in a mass ratio of 1:2:3), 3 parts of fatty acid methyl ester polyether sulfonate, 4 parts of a composite surfactant (trisiloxane and sodium fatty alcohol polyoxyethylene ether sulfate in a mass ratio of 1:1), 2 parts of magnesium aluminum silicate, 3 parts of sodium polynaphthaldehyde sulfonate, 2 parts of sodium benzoate and 75 parts of water were mixed, and the mixture was stirred at a speed of 5000 r / min for 45 min to obtain a control agent for damping-off disease.

[0051] Comparative Example 1:

[0052] The difference between Comparative Example 1 and Example 3 is that the composite antibacterial agent in Comparative Example 1 is a mixture of thiamethoxam, myclobutanil and ipconazole in a mass ratio of 1:1:7, and the rest is the same as Example 3.

[0053] Comparative Example 2:

[0054] The difference between Comparative Example 2 and Example 3 is that the composite antibacterial agent in Comparative Example 2 is a mixture of thiamethoxam, myclobutanil and ipconazole in a mass ratio of 7:1:1, and the rest is the same as Example 3.

[0055] Comparative Example 3:

[0056] The difference between Comparative Example 3 and Example 3 is that the composite antibacterial agent in Comparative Example 3 is a mixture of thiamethoxam, myclobutanil and ipconazole in a mass ratio of 1:7:1, and the rest is the same as Example 3.

[0057] Comparative Example 4:

[0058] The difference between Comparative Example 4 and Example 3 is that the composite antibacterial agent in Comparative Example 4 is a mixture of thiamethoxam and myclobutanil in a mass ratio of 2:7, and the rest is the same as Example 3.

[0059] Comparative Example 5:

[0060] The difference between Comparative Example 5 and Example 3 is that the composite antibacterial agent in Comparative Example 5 is a mixture of myclobutanil and ipconazole in a mass ratio of 7:2, and the rest is the same as Example 3.

[0061] Comparative Example 6:

[0062] The difference between Comparative Example 6 and Example 3 is that no composite plant immune inducer is added in Comparative Example 6, and the rest is the same as Example 3.

[0063] Comparative Example 7:

[0064] The difference between Comparative Example 7 and Example 3 is that in Comparative Example 7, a single chitosan oligosaccharide is added as a plant immunity inducer, and the rest is the same as Example 3.

[0065] Comparative Example 8:

[0066] The difference between Comparative Example 8 and Example 3 is that no plant growth regulator is added in Comparative Example 8, and the rest is the same as Example 3.

[0067] Comparative Example 9:

[0068] The difference between Comparative Example 9 and Example 3 is that the plant growth regulator in Comparative Example 9 is potassium dihydrogen phosphate and ethephon in a mass ratio of 4:2, and the rest is the same as Example 3.

[0069] Comparative Example 10:

[0070] The difference between Comparative Example 10 and Example 3 is that the plant growth regulator in Comparative Example 10 is potassium dihydrogen phosphate and magnolol in a mass ratio of 2:4, and the rest is the same as Example 3.

[0071] Comparative Example 11:

[0072] The difference between Comparative Example 11 and Example 3 is that no composite surfactant is added in Comparative Example 11, and the rest is the same as Example 3.

[0073] Comparative Example 12:

[0074] The difference between Comparative Example 12 and Example 3 is that the surfactant in Comparative Example 12 is a single trisiloxane, and the rest is the same as Example 3.

[0075] Comparative Example 13:

[0076] The difference between Comparative Example 13 and Example 3 is that the surfactant in Comparative Example 13 is a single fatty alcohol polyoxyethylene ether sodium sulfate, and the rest is the same as Example 3.

[0077] Comparative Example 14:

[0078] The difference between Comparative Example 14 and Example 3 is that the composition ratio of the control agent in Comparative Example 14 is different, as follows, and the rest is the same as Example 3.

[0079] By weight, 16 parts of a composite antibacterial agent (thiamethoxam, myclobutanil and ioconazole in a mass ratio of 3:1:4), 5 parts of a composite plant immune inducer (chitosan oligosaccharide and benzothiadiazole in a mass ratio of 4:1), 3 parts of a plant growth regulator (potassium dihydrogen phosphate, ethephon and magnolol in a mass ratio of 1:2:3), 6 parts of fatty acid methyl ester polyether sulfonate, 9 parts of a composite surfactant (trisiloxane and fatty alcohol polyoxyethylene ether sodium sulfate in a mass ratio of 1:1), 1 part of magnesium aluminum silicate, 1 part of sodium polynaphthaldehyde sulfonate, 3 parts of sodium benzoate and 55 parts of water were mixed, and the mixture was stirred at a speed of 5000 r / min for 45 min to obtain a control agent for damping-off disease.

[0080] 1. Use the indoor toxicity determination method (according to Sun Yunpei's method) to determine the co-toxicity coefficient (CTC value) of various compound mixtures through indoor toxicity determination, and use the CTC value to judge the synergistic effect of the mixture.

[0081] Actual toxicity index ATI = standard drug LD 50 / Mixed Agent LD 50 *100;

[0082] Toxicity index TTI = standard drug LD 50 / Test drug LD 50 *100;

[0083] Co-toxicity coefficient CTC = ATI / TTI*100.

[0084] Table 1: Indoor co-toxicity coefficient test results

[0085]

[0086] As can be seen from Table 1, although only part of the toxicity test results are provided, Table 1 also shows that the compounding of thiamethoxam, azoxystrobin and picronil in the present application can synergize and enhance the efficacy. Therefore, the compounding and compounding ratio of thiamethoxam, azoxystrobin and picronil in the present application are preferred, and subsequent experiments are continued.

[0087] 2. Test on the control effect of rice thrips

[0088] Method: After diluting the control agent prepared in Examples 1 to 3 and the control agent prepared in Comparative Examples 1 to 14 by 200 times, spray control at a dosage of 150 mL / mu, and repeat 4 times. The base number of rice thrips was investigated before application, and the residual insect quantity was investigated 2 days and 7 days after application, for a total of 3 investigations.

[0089] Control effect (%) = (pest population reduction rate in the pesticide treatment area - pest population reduction rate in the blank control area) / (100 - pest population reduction rate in the blank control area) × 100. The results are shown in Table 2 below.

[0090] Table 2: Results of rice thrips pest control

[0091]

[0092] From the data analysis of Table 2, it can be seen that after the components are compounded, the present invention has a significant control effect on rice thrips pests.

[0093] 3. The control agents prepared in Examples 1 to 3 and the control agents in Comparative Examples 11 to 13 were diluted 200 times and then dripped onto rice leaves to observe the spreading effect. The results are shown in Table 3 below.

[0094] Table 3: Spreading effect

[0095]

[0096] From the data analysis of Table 3, it can be seen that the present invention, by adding a composite surfactant into the formula, helps to promote the spreading of the control agent on the rice leaves, and can quickly diffuse on the leaves, effectively improving the utilization rate of the control agent.

[0097] 4. Test on the control effect of damping-off disease

[0098] The test subjects were rice seedlings infected with damping-off disease but not yet in ear form;

[0099] The control agents of Examples 1 to 3 and the control agents of Comparative Examples 1 to 14 were diluted 200 times with water and sprayed on rice seedlings at a dosage of 150 mL / mu. After 10 days, the control effect was statistically analyzed, and the blank control was made by applying an equal amount of clean water. The results are shown in Table 4. Relative control effect (%) = [(control diseased plant rate - treatment diseased plant rate) / control diseased plant rate] × 100.

[0100] Table 4: Control effect of damping-off disease

[0101]

[0102]

[0103] From the data analysis of Table 4, it can be seen that the compounding of the components in the present invention can help improve the control effect on rice damping-off disease.

[0104] 5. Growth promotion verification

[0105] The rice seedlings of the test group of Examples 1 to 3 where the control effect test was completed, the rice seedlings of the test group of Comparative Examples 6 to 14 where the control effect test was completed, and the rice seedlings of the blank control group were selected and cultured for 10 days to observe the growth of the rice seedlings, and to test the plant height, root length, fresh and dry weight of the aboveground part, and fresh and dry weight of the underground part of the rice seedlings. The results are shown in Table 5.

[0106] Table 5: Characterization of growth promoting performance

[0107]

[0108] From the data analysis of Table 5, it can be seen that after verifying the control effect of the present invention on rice seedlings infected with damping-off disease, further cultivation research found that after the ingredients are compounded, the control agent has an excellent growth-promoting effect.

[0109] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0110] The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A control agent for damping-off disease, characterized in that: The damping-off disease prevention and control agent comprises the following raw materials in parts by weight: 9 to 17 parts of a composite antibacterial agent, 7 to 15 parts of a composite plant immune inducer, 1 to 3 parts of a plant growth regulator, 1 to 3 parts of an emulsifier, 2 to 5 parts of a composite surfactant, 1 to 3 parts of a thickener, 1 to 3 parts of a dispersant, 1 to 3 parts of a preservative, and 45 to 80 parts of water; Wherein, the composite antibacterial agent is obtained by mixing thiamethoxam, azoxystrobin and ipconazole; The composite plant immune inducer is obtained by mixing chitosan oligosaccharide and benzothiadiazole; The plant growth regulator is obtained by mixing potassium dihydrogen phosphate, ethephon and magnolol.

2. The control agent according to claim 1, characterized in that: The mass ratio of thiamethoxam, myclobutanil and ipconazole is (1-5): (1-3): (1-5).

3. The control agent according to claim 1, characterized in that: The mass ratio of the chitosan oligosaccharide to benzothiadiazole is (1-7): (1-2).

4. The control agent according to claim 1, characterized in that: The mass ratio of potassium dihydrogen phosphate, ethephon and magnolol is (1-3): (1-2): (1-5).

5. The control agent according to claim 1, characterized in that: The emulsifier is at least one of trialkylphenol polyoxyethylene ether, phenethylphenol polyoxyethylene ether and fatty acid methyl ester polyether sulfonate.

6. The control agent according to claim 1, characterized in that: The composite surfactant comprises trisiloxane and fatty alcohol polyoxyethylene ether sodium sulfate in a mass ratio of (1-3):(1-7).

7. The control agent according to claim 1, characterized in that: The thickener is at least one of xanthan gum, magnesium aluminum silicate and sodium alginate.

8. The control agent according to claim 1, characterized in that: The dispersant is at least one of polynaphthalene formaldehyde sulfonate sodium salt, sodium methylene bisnaphthalene sulfonate and sodium hexametaphosphate.

9. The control agent according to claim 1, characterized in that: The preservative is at least one of sorbic acid, sodium benzoate and kasone.

10. A method for preparing a drug for preventing and treating damping-off disease, characterized in that: The preparation method is used to prepare the control agent for damping-off disease according to any one of claims 1 to 9, and the preparation method comprises the following steps: The composite antibacterial agent, the composite plant immune inducer, the plant growth regulator, the emulsifier, the composite surfactant, the thickener, the dispersant, the preservative and water are mixed by weight, and stirred at a rotation speed of 2000 r / min to 15000 r / min for 20 min to 45 min to obtain a control agent for damping-off disease.

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