Water-soluble emulsion for aquatic products and preparation method thereof

Abstract

The invention discloses a water-soluble emulsion for aquatic products and a preparation method thereof, and relates to the field of feed additive.According to the water-soluble emulsion, an antioxidant is added, emulsification treatment is adopted, an oil-in-water state is formed, the stability of organic acid, vitamins and essential oil can be protected, and digestion and absorption are facilitated; furthermore, the traditional Chinese medicine feed raw materials have the effects of detoxifying, protecting the liver and improving the immunity, after probiotic fermentation treatment is adopted, dissolution of effective components of traditional Chinese medicines can be promoted, the effect can be enhanced, probiotics can be generated through fermentation, the taste is improved, vitamins and essential oil are matched, the overall bioavailability is improved, and the traditional Chinese medicine feed is suitable for large-scale industrial production and the like.

Description

Technical Field

[0001] The present invention relates to the field of feed additives, in particular to a water-soluble emulsion for aquatic products and a preparation method thereof, and specifically to a water-soluble emulsion of plant essential oil, acidulant and vitamin containing Chinese medicine (probiotic fermentation) ingredients for aquatic products and a preparation method thereof. Background Art

[0002] With the accelerated pace of banning and replacing antibiotics, the status of traditional Chinese medicine, probiotics and natural plant extracts in animal farming has become increasingly prominent. Among them, acidifiers, plant essential oils and vitamins are recognized as typical representatives of non-toxic, harmless and pollution-free green additives, and are one of the current research focuses and hotspots. Water quality and breeding environment management is one of the technical difficulties in aquatic animal breeding. Feed residues, animal excrement, algae, etc. in the breeding process will cause water quality to deteriorate, affecting the health of aquatic animals, especially the health of the liver and intestines.

[0003] Composite acidifiers are used in farmed animals to make up for the lack of physiological acid production in aquatic animals, thereby alleviating problems such as poor digestion and absorption, nutritional diarrhea, and feed waste in aquatic animals. Since a single ordinary acidifier adopts a simple mixing or adsorption process, the exposed acid is easy to react with the alkaline substances in the aquatic feed and lose its antibacterial effect. Therefore, composite acidifiers will also replace single acidifiers and become the development trend of acidifiers; plant essential oils have good antibacterial and antiviral properties, and can improve the antibacterial effect whether added alone or used in combination with antibiotics. However, plant essential oils have the disadvantages of being easy to oxidize and volatilize. Simple mixed plant essential oils are difficult to achieve the corresponding effect due to their instability, so essential oils need to be specially treated or combined with other materials; the ingredients in traditional Chinese medicine are very complex, and there are many effective ingredients. How to reasonably match the commonly used traditional Chinese medicines for liver protection in aquatic products, how to treat them more effectively, in the face of these existing defects, how to find a combination method to maximize the efficacy of the product is particularly important. Summary of the invention

[0004] In order to overcome the shortcomings of the background technology, the present invention provides a water-soluble emulsion for aquatic products and a preparation method thereof. The present invention integrates complex vitamins, acidulants, plant essential oils, and fermented Chinese medicines, reasonably combines them, gives full play to the comprehensive value of the products, makes up for the application defects of a single variety, and adopts homogenizing emulsification technology to make the water-soluble emulsion, which is more convenient to use in ponds.

[0005] In order to achieve the above-mentioned purpose of the invention, the present invention adopts the following technical solution: A water-soluble emulsion for aquatic products, the water-soluble emulsion comprising the following components by mass percentage: Citric acid 5-10%; Fumaric acid 10-20%; Sodium benzoate 3-6%; Medium chain fatty acids 5-10%; Cinnamaldehyde 3-6%; Oregano oil 1-3%; Eucalyptus oil 2-4%; Antioxidants 3-6%; Emulsifier 15-20%; Vitamin C 3-6%; Vitamin D 1-3%; Vitamin E 2-4%; Vitamin K 1-3%; Liquid probiotics Chinese medicine fermentation product 15-30%.

[0006] In the water-soluble emulsion for aquatic products, the antioxidant is any one of disodium EDTA, phytic acid, BHT, glycyrrhizin or astaxanthin, or a combination of two or more thereof.

[0007] In the water-soluble emulsion for aquatic products, when the antioxidant is a combination of disodium edetate, phytic acid, BHT, glycyrrhizin and astaxanthin, the addition ratio of disodium edetate, phytic acid, BHT, glycyrrhizin and astaxanthin is (0-1): (0.5-1): 2: (0.5-3): (0.5-1).

[0008] The water-soluble emulsion for aquatic products, wherein the emulsifier is any one of lecithin, propylene glycol or Tween 80, or a combination of two or more thereof.

[0009] In the water-soluble emulsion for aquatic products, when the emulsifier is a combination of lecithin, propylene glycol and Tween 80, the addition ratio of lecithin, propylene glycol and Tween 80 is 1: (0-1): (1-1.5).

[0010] The water-soluble emulsion for aquatic products, the Chinese medicine carrier in the liquid probiotic Chinese medicine fermentation product is any one of licorice, astragalus, eucommia leaf or milk thistle powder, or a combination of two or more thereof.

[0011] In the water-soluble emulsion for aquatic products, the substitute for astragalus is roasted licorice.

[0012] In the water-soluble emulsion for aquatic products, when the Chinese medicine carrier is a combination of licorice, astragalus, eucommia leaves and milk thistle fine powder, the addition ratio of licorice, astragalus, eucommia leaves and milk thistle fine powder is 4: (0-1): (0.5-1.5): 0.5.

[0013] The water-soluble emulsion for aquatic products, the probiotics in the liquid probiotic Chinese medicine fermentation product are any one or a combination of two or more of EM bacteria, lactobacillus, fecal streptococcus, bacillus or yeast. When the probiotics are a combination of EM bacteria, lactobacillus, fecal streptococcus, bacillus and yeast, the addition ratio of EM bacteria, lactobacillus, fecal streptococcus, bacillus and yeast is (0-1): (10-100): (0-1): (0-10): 1.

[0014] A method for preparing a water-soluble emulsion for aquatic products, the preparation method specifically comprising the following steps: The first step is to add the probiotics in the liquid probiotic Chinese medicine fermentation product to the Chinese medicine carrier mixture in proportion, add water, control the moisture content to 35-45%, put it into a fermentation bag, place it in a greenhouse at a temperature of 30-35°C, ferment for 5-9 days, boil it with one times the amount of water for 10-20 minutes, and take the supernatant as the liquid probiotic Chinese medicine fermentation product; Step 2: Add citric acid, fumaric acid, sodium benzoate, vitamin C, antioxidants, and emulsifiers to the supernatant solution obtained in the first step, stir evenly, and set aside; Step 3: Mix cinnamaldehyde, oregano oil, eucalyptus oil, medium-chain fatty acids, vitamin D, vitamin E and vitamin K and stir evenly for later use; Step 4: Add the mixed liquids obtained in the second and third steps into a homogenizer in sequence, with the homogenization conditions being a rotation speed of 10,000 to 13,000 rpm and a temperature of 40 to 50° C., until a uniform and transparent emulsion is formed.

[0015] By adopting the above technical solution, the present invention has the following advantages: In addition to adding antioxidants, the present invention adopts emulsification treatment to form an oil-in-water state, which can protect the stability of organic acids, vitamins, and essential oils and help digestion and absorption; Chinese medicinal feed raw materials themselves have the effects of detoxification, liver protection and improving immunity. After being fermented with probiotics, they can promote the dissolution of effective ingredients of Chinese medicine and enhance this effect. Fermentation can also produce probiotics and improve the taste.

[0016] Combined with vitamins and essential oils, it improves overall bioavailability and is suitable for large-scale industrial production. DETAILED DESCRIPTION

[0017] The present invention can be explained in more detail by the following examples, but the present invention is not limited to the following examples; The present invention mainly explores the preparation of acidulants, plant essential oils, vitamins and Chinese medicine (fermentable) and the processing forms during preparation, and makes the four of them play the maximum effect to solve the water quality and health problems existing in aquatic animal breeding.

[0018] The principle of the present invention is that vitamins and plant essential oils are easily oxidized. In addition to adding antioxidants, nano-emulsification treatment is used to form a water-in-oil state, which can protect the stability of organic acids, vitamins, and essential oils and help digestion and absorption. The Chinese medicine feed raw materials are fermented with probiotics to promote the dissolution of effective ingredients of Chinese medicine, produce probiotics and improve the taste. Vitamins and essential oils are combined to improve the overall bioavailability.

[0019] It should be noted that if medium-chain fatty acids are added to the existing formula, medium-chain fatty acids (MCFA) refer to saturated fatty acids containing 6 to 12 carbon atoms, which have the characteristics of short carbon chains, small molecules, high polarity and water solubility. It has characteristics in digestion, absorption, transportation, oxidation, etc. in animals, and can play a role in rapid energy supply, improving fat deposition, improving insulin sensitivity, improving the quality of livestock products, inhibiting bacteria, and adjusting intestinal flora and structure. MCFA is oxidized in liver cells to produce a large amount of acetyl CoA. Acetyl CoA can be used with citric acid in the formula to carry out tricarboxylic acid cycle in mitochondria, and quickly oxidize to provide energy. At the same time, ketone bodies are also generated and enter the blood, which increases the ketone body content in the blood. Ketone bodies can be used as energy substances by tissues such as myocardium, skeletal muscle and brain, so it can reduce the consumption of glycogen and protein in the body and reduce nitrogen excretion. MCFA is rarely re-esterified into MCT, so direct feeding of MCFA will not affect the composition of animal body fat fatty acids. MCFA, due to its unique antibacterial principle, makes it difficult for bacteria to develop drug resistance. Using it to replace antibiotics may become one of the important ways in the future. Medium-chain fatty acids work best when they work in synergy with other acids and essential oils in the formula. The fermented formula of traditional Chinese medicine has the effect of detoxification and liver protection. After fermentation, probiotics can enhance the bioavailability of traditional Chinese medicine ingredients and produce probiotics. The combined effect greatly enhances the body's immunity.

[0020] The technical problems solved by the present invention are as follows: 1. Solved the problem of incomplete efficacy of a single ingredient and increased difficulty in application; 2. Improve water quality and enhance the immunity of aquatic animals (probiotic Chinese medicine fermentation combined with essential oils can degrade residual bait, feces and organic matter at the bottom of the pond, reduce sedimentation; reduce the content of nitrite, ammonia nitrogen and hydrogen sulfide in the water; inhibit the growth of algae and microorganisms, increase dissolved oxygen; regulate intestinal health, enhance liver detoxification ability, and reduce immunosuppression); 3. It does not produce any toxic substances and is more environmentally friendly (the raw materials used are safe and non-toxic green additives, and probiotics ferment to produce prebiotics, small molecule peptides, amino acids and other beneficial substances) 4. Improve the quality of aquatic animals and ensure animal health (astaxanthin, vitamins and other substances help animals improve their stress resistance and survival rate, and astaxanthin accumulates in aquatic animals to improve their quality).

[0021] A water-soluble emulsion for aquatic products, the water-soluble emulsion comprising the following components by mass percentage: Citric acid 5-10%; Fumaric acid 10-20%; Sodium benzoate 3-6%; Medium chain fatty acids 5-10%; Cinnamaldehyde 3-6%; Oregano oil 1-3%; Eucalyptus oil 2-4%; Antioxidant 3-6%; Emulsifier 15-20%; Vitamin C 3-6%; Vitamin D 1-3%; Vitamin E 2-4%; Vitamin K 1-3%; liquid probiotic Chinese medicine fermentation product 15-30%.

[0022] In implementation, the antioxidant is any one of disodium edetate or phytic acid or BHT or glycyrrhizin or astaxanthin, or a combination of two or more thereof. Further, when the antioxidant is a combination of disodium edetate, phytic acid, BHT, glycyrrhizin and astaxanthin, the addition ratio of disodium edetate, phytic acid, BHT, glycyrrhizin and astaxanthin is (0-1): (0.5-1): 2: (0.5-3): (0.5-1).

[0023] Furthermore, the emulsifier is any one of lecithin, propylene glycol or Tween 80, or a combination of two or more thereof.

[0024] Furthermore, when the emulsifier is a combination of lecithin, propylene glycol and Tween 80, the addition ratio of lecithin, propylene glycol and Tween 80 is 1: (0-1): (1-1.5).

[0025] Furthermore, the Chinese medicine carrier in the liquid probiotic Chinese medicine fermentation product is any one of licorice, astragalus, eucommia leaf, or milk thistle powder, or a combination of two or more thereof.

[0026] Furthermore, the substitute for Astragalus is roasted liquorice.

[0027] Furthermore, when the Chinese medicine carrier is a combination of licorice, astragalus, eucommia leaves, and milk thistle fine powder, the addition ratio of licorice, astragalus, eucommia leaves, and milk thistle fine powder is 4: (0-1): (0.5-1.5): 0.5.

[0028] Furthermore, the probiotics in the liquid probiotic Chinese medicine fermentation product are any one of EM bacteria or lactobacillus or fecal streptococcus or bacillus or yeast, or a combination of two or more thereof. When the probiotics are a combination of EM bacteria, lactobacillus, fecal streptococcus, bacillus and yeast, the addition ratio of EM bacteria, lactobacillus, fecal streptococcus, bacillus and yeast is (0-1):(10-100):(0-1):(0-10):1.

[0029] A method for preparing a water-soluble emulsion for aquatic products, the preparation method specifically comprising the following steps: The first step is to add the probiotics in the liquid probiotic Chinese medicine fermentation product to the Chinese medicine carrier mixture in proportion, add water, control the moisture content to 35-45%, put it into a fermentation bag, place it in a greenhouse at a temperature of 30-35°C, and ferment it for 5-9 days, boil it with one times the amount of water for 10-20 minutes, and take the supernatant as the liquid probiotic Chinese medicine fermentation product; when adding water, it is best to control the moisture content to 40%; the fermentation bag is a bag with a breathing valve, which is a commonly used bag in the art and will not be described here; the fermentation days are preferably 7 days, and the boiling time is preferably 15 minutes; Step 2: Add appropriate amount of citric acid, fumaric acid, sodium benzoate, vitamin C, antioxidant and emulsifier to the supernatant solution obtained in the first step, stir evenly and set aside; Step 3: Mix appropriate amounts of cinnamaldehyde, oregano oil, eucalyptus oil, medium-chain fatty acids, vitamin D, vitamin E and vitamin K and stir evenly for later use; Step 4: Add the mixed liquids obtained in the second and third steps into a homogenizer in sequence, with the homogenization conditions being a rotation speed of 10,000 to 13,000 rpm and a temperature of 40 to 50° C., until a uniform and transparent emulsion is formed.

[0030] During implementation, the stirring time in the second step and the third step is not less than 10 minutes.

[0031] The specific embodiments of the present invention are as follows: Example 1: A water-soluble emulsion for aquatic products, the water-soluble emulsion comprising the following components by mass percentage: Citric acid 5.0%, fumaric acid 10.0%, sodium benzoate 4.0%, caproic acid 2.0%, lauric acid 3.0%, cinnamaldehyde 3.0%, oregano oil 1.0%, eucalyptus oil 2.0%; BHT 2.0%, glycyrrhizin 0.5%, astaxanthin 0.5%, propylene glycol 6.0%, Tween 80 6.0%, lecithin 3.0%, vitamin C 3.0%, vitamin D 1.0%, vitamin E 2.0%, vitamin K 1.0%, liquid probiotics Chinese medicine fermentation 15.0%, the balance is water.

[0032] Implementation steps: ① Add EM bacteria (including photosynthetic bacteria) and Lactobacillus plantarum to licorice, astragalus, eucommia leaf, and milk thistle powder (4:0.5:0.5:0.5) in a ratio of 1:10, add water (the moisture content is controlled at 35%), put it into a fermentation bag (a bag with a breathing valve), place it in a greenhouse at a temperature of 30-35°C, and after fermentation for 5 days, boil it with one times the amount of water for 10 minutes, and take the supernatant as the liquid probiotic Chinese medicine fermentation product; ② Mix cinnamaldehyde, oregano oil, eucalyptus oil, BHT, caproic acid, lauric acid, vitamin D, vitamin E, vitamin K, propylene glycol, Tween 80 and astaxanthin, and place in a 40-50℃ water bath if necessary, mix well and set aside; ③ Mix citric acid, fumaric acid, sodium benzoate and vitamin C evenly, then add them into the liquid probiotic Chinese medicine fermentation product and stir evenly; ④ Add the suspension of step 2 to the solution of step 3 and mix well; ⑤ Place the solution in step 4 in a homogenizer and emulsify for 30 minutes at a temperature of 40°C and a speed of 10,000 rpm until a uniform and stable aquatic product emulsion is obtained.

[0033] Example 2: Citric acid 7.5%, fumaric acid 15.0%, sodium benzoate 4.0%, caproic acid 2.0%, lauric acid 4.0%, cinnamaldehyde 4.0%, oregano oil 2.0%, eucalyptus oil 3.0%; BHT 2.0%, glycyrrhizin 0.5%, astaxanthin 0.5%, propylene glycol 6.0%, Tween 80 8.0%, lecithin 3.0%, vitamin C 4.0%, vitamin D 2.0%, vitamin E 3.0%, vitamin K 2.0%, liquid probiotics Chinese medicine fermentation 20.0%, the balance is water.

[0034] Implementation steps: ① Add EM bacteria (including photosynthetic bacteria), Lactobacillus plantarum, Streptococcus faecalis, and yeast in a ratio of 1:100:1:1 to licorice, astragalus, eucommia leaf, and milk thistle powder (4:0.5:0.5:0.5), add water (the moisture content is controlled at 40%), put it into a fermentation bag (a bag with a breathing valve), place it in a greenhouse at a temperature of 30-35°C, and after fermentation for 7 days, boil it with one times the amount of water for 15 minutes, and take the supernatant as the liquid probiotic Chinese medicine fermentation product; ② Mix cinnamaldehyde, oregano oil, eucalyptus oil, BHT, caproic acid, lauric acid, vitamin D, vitamin E, vitamin K, propylene glycol, Tween 80 and astaxanthin, and place in a 40-50℃ water bath if necessary, mix well and set aside; ③ Mix citric acid, fumaric acid, sodium benzoate and vitamin C evenly, then add them into the liquid probiotic Chinese medicine fermentation product and stir evenly; ④ Add the suspension of step 2 to the solution of step 3 and mix well; ⑤ Place the solution in step 4 in a homogenizer and emulsify for 30 minutes at a temperature of 45°C and a speed of 12000 rpm until a uniform and stable aquatic product emulsion is obtained.

[0035] Example 3: Citric acid 7.5%, fumaric acid 15.0%, sodium benzoate 4.0%, caproic acid 2.0%, caprylic acid 2.0%, lauric acid 4.0%, cinnamaldehyde 5.0%, oregano oil 2.0%, eucalyptus oil 3.0%; BHT 2.0%, glycyrrhizin 0.5%, astaxanthin 0.5%, propylene glycol 6.0%, Tween 80 8.0%, lecithin 3.0%, vitamin C 3.5%, vitamin D 2.0%, vitamin E 3.0%, vitamin K 2.0%, liquid probiotics Chinese medicine fermentation 25.0%.

[0036] Implementation steps: ① Add EM bacteria (including photosynthetic bacteria), Lactobacillus plantarum, Streptococcus faecalis, Bacillus, and yeast in a ratio of 1:100:10:1:1 to licorice, roasted licorice, Eucommia ulmoides leaves, and milk thistle powder (4:0.5:0.5:0.5), add water (the moisture content is controlled at 45%), put it into a fermentation bag (a bag with a breathing valve), place it in a greenhouse at a temperature of 30-35°C, and after fermentation for 9 days, boil it with one times the amount of water for 20 minutes, and take the supernatant as the liquid probiotic Chinese medicine fermentation product; ② Mix cinnamaldehyde, oregano oil, eucalyptus oil, BHT, caproic acid, lauric acid, vitamin D, vitamin E, vitamin K, propylene glycol, Tween 80 and astaxanthin, and place in a 50℃ water bath if necessary, mix well and set aside; ③ Mix citric acid, fumaric acid, sodium benzoate and vitamin C evenly, then add them into the liquid probiotic Chinese medicine fermentation product and stir evenly; ④ Add the suspension of step 2 to the solution of step 3 and mix well; ⑤ Place the solution in step 4 in a homogenizer and emulsify for 30 minutes at a temperature of 45°C and a speed of 13000 rpm until a uniform and stable aquatic product emulsion is obtained.

[0037] The application data on white shrimp is as follows:

[0038] The control group was fed normally. Compared with the control group, the embodiment scheme increased the weight gain rate of whiteleg shrimp to varying degrees, and the corresponding survival rate was also increased accordingly.

[0039] Parts of the present invention not described in detail are prior art.

[0040] The embodiments selected herein for the purpose of disclosing the invention are currently considered to be suitable, but it should be understood that the invention is intended to include all changes and modifications of the embodiments that fall within the scope of the concept and invention.

Claims

1. A water-soluble emulsion for aquatic products, characterized by: The water-soluble emulsion comprises the following components by mass percentage: Citric acid 5-10%; Fumaric acid 10-20%; Sodium benzoate 3-6%; Medium chain fatty acids 5-10%; Cinnamaldehyde 3-6%; Oregano oil 1-3%; Eucalyptus oil 2-4%; Antioxidants 3-6%; Emulsifier 15-20%; Vitamin C 3-6%; Vitamin D 1-3%; Vitamin E 2-4%; Vitamin K 1-3%; Liquid probiotics Chinese medicine fermentation product 15-30%.

2. The water-soluble emulsion for aquatic products according to claim 1, characterized in that: The antioxidant is any one of disodium EDTA, phytic acid, BHT, glycyrrhizin or astaxanthin, or a combination of two or more thereof.

3. The water-soluble emulsion for aquatic products according to claim 2, characterized in that: When the antioxidant is a combination of disodium edetate, phytic acid, BHT, glycyrrhizin and astaxanthin, the addition ratio of disodium edetate, phytic acid, BHT, glycyrrhizin and astaxanthin is (0-1): (0.5-1): 2: (0.5-3): (0.5-1).

4. The water-soluble emulsion for aquatic products according to claim 1, characterized in that: The emulsifier is any one of lecithin, propylene glycol or Tween 80, or a combination of two or more thereof.

5. The water-soluble emulsion for aquatic products according to claim 4, characterized in that: When the emulsifier is a combination of lecithin, propylene glycol and Tween 80, the addition ratio of lecithin, propylene glycol and Tween 80 is 1: (0-1): (1-1.5).

6. The water-soluble emulsion for aquatic products according to claim 1, characterized in that: The Chinese medicine carrier in the liquid probiotic Chinese medicine fermentation product is any one of licorice, astragalus, eucommia leaf or milk thistle powder, or a combination of two or more thereof.

7. The water-soluble emulsion for aquatic products according to claim 1, characterized in that: The substitute of Astragalus is Radix Glycyrrhizae.

8. The water-soluble emulsion for aquatic products according to claim 1, characterized in that: When the Chinese medicine carrier is a combination of licorice, astragalus, eucommia leaves and milk thistle powder, the addition ratio of licorice, astragalus, eucommia leaves and milk thistle powder is 4: (0-1): (0.5-1.5): 0.

5.

9. The water-soluble emulsion for aquatic products according to claim 1, characterized in that: The probiotics in the liquid probiotic Chinese medicine fermentation product are any one of EM bacteria, lactobacillus, fecal streptococcus, bacillus or yeast, or a combination of two or more thereof. When the probiotics are a combination of EM bacteria, lactobacillus, fecal streptococcus, bacillus and yeast, the addition ratio of EM bacteria, lactobacillus, fecal streptococcus, bacillus and yeast is (0-1): (10-100): (0-1): (0-10):

1.

10. A method for preparing a water-soluble emulsion for aquatic products according to any one of claims 1 to 9, characterized in that: The preparation method specifically comprises the following steps: The first step is to add the probiotics in the liquid probiotic Chinese medicine fermentation product to the Chinese medicine carrier mixture in proportion, add water, control the moisture content to 35-45%, put it into a fermentation bag, place it in a greenhouse at a temperature of 30-35°C, ferment for 5-9 days, boil it with one times the amount of water for 10-20 minutes, and take the supernatant as the liquid probiotic Chinese medicine fermentation product; Step 2: Add citric acid, fumaric acid, sodium benzoate, vitamin C, antioxidants, and emulsifiers to the supernatant solution obtained in the first step, stir evenly, and set aside; Step 3: Mix cinnamaldehyde, oregano oil, eucalyptus oil, medium-chain fatty acids, vitamin D, vitamin E and vitamin K and stir evenly for later use; Step 4: Add the mixed liquids obtained in the second and third steps into a homogenizer in sequence, with the homogenization conditions being a rotation speed of 10,000 to 13,000 rpm and a temperature of 40 to 50° C., until a uniform and transparent emulsion is formed.

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