Composite dampness-clearing nutrition powder prepared by biological wall breaking technology

Abstract

The invention discloses composite dampness-clearing nutrition powder prepared by a biological wall breaking technology, and belongs to the technical field of food processing. The dampness-clearing nutritional powder composition contains ginger, cinnamon, girl red date wine powder, coix seeds, gordon euryale seeds, astragalus membranaceus, fructus piperis longi, apple powder, citrus fiber powder, lactic acid bacteria, radix fici simplicissimae, cistanche, poria cocos, lalang grass rhizome, ginseng and the like, and the dampness-clearing nutritional powder composition with remarkable effects is synthesized by adopting a biological wall breaking technology and a fermentation technology.

Description

Technical Field

[0001] The invention belongs to the technical field of food processing, and in particular relates to a composite moisture-removing nutrient powder prepared by a biological wall-breaking technology. Background Art

[0002] Dampness is the most common pathogenic factor, which can be divided into internal and external. External dampness is one of the six evils, and often hurts the lower body first. It is mostly caused by external dampness such as humid climate, wading in water and rain, and living in a damp place. Internal dampness is both a pathological product and a pathogenic factor. Internal dampness is mostly caused by the spleen failing to function properly and water and dampness stagnation. Although external dampness and internal dampness are different, they often affect each other during the course of the disease. When external dampness causes disease, it often affects the spleen and stomach, causing the spleen to fail to function properly and dampness to be generated from the inside; and when the spleen fails to function properly, it is easy to be invaded by external dampness.

[0003] No matter what kind of dampness it is, it will cause people to have symptoms of decreased digestive function such as loss of appetite, abdominal distension, diarrhea, and often accompanied by mental fatigue, drowsiness, physical fatigue, bloating, loss of water, white and greasy tongue coating, etc. This series of symptoms are all related to "excessive dampness and turbidity inside the body".

[0004] Therefore, in view of the above problems, it is necessary to develop a dehumidifying nutrient powder composition with simple ingredients and significant efficacy. Summary of the invention

[0005] In order to overcome the above technical problems, the purpose of the present invention is to provide a composite dehumidifying nutrient powder prepared by biological wall breaking technology.

[0006] To achieve the above objectives, the technical solution provided by the present invention is as follows:

[0007] The composite dehumidifying nutrient powder prepared by biological wall breaking technology, the preparation method of the composite dehumidifying nutrient powder comprises the following steps:

[0008] (1) Biological cell wall breaking: ginger, cinnamon, red date wine powder, coix seed, Euryale ferox, astragalus, longan, apple powder, citrus fiber powder, quinquefolius, cistanche deserticola, poria, Imperata root, and ginseng powder are mixed in proportion, water is added, and a microbial preparation is added for biological cell wall breaking to obtain a cell wall breaking mixture;

[0009] (2) fermentation: adding lactic acid bacteria to the cell wall-broken mixture and fermenting to obtain a fermentation mixture;

[0010] (3) Filtration and concentration: After adjusting the pH of the fermentation mixture, the mixture is filtered, and the filtrate is concentrated and dried to obtain nutrient powder;

[0011] The components of the composite dehumidifying nutrient powder include: ginger, cinnamon, girls' red date wine powder, coix seed, euryale ferox, astragalus, longan, apple powder, citrus fiber powder, lactic acid bacteria, quinquefolius, cistanche, poria, white grass root, ginseng, and the mass ratio of each component is: 0.5-1:1-2:5-8:0.4-1:0.5-0.8:0.5-1.2:1-1.5:1-1.5:0.7-1.2:2.5-3:1.5-2:1-1.5:1-2:0.5-0.7:08-1.3.

[0012] Preferably, the biological cell wall disruption time in step (1) is 2-3 hours.

[0013] Preferably, the microbial agent in step (1) is one or more of yeast, mold, and actinomycetes.

[0014] Preferably, the amount of microbial agent used in step (1) is 10-15% of the total components.

[0015] Preferably, in step (2), the fermentation temperature is 22-28° C. and the fermentation time is 12-24 h.

[0016] Preferably, the pH value in step (3) is 6-8.

[0017] Cistanche deserticola: Cistanche deserticola is a traditional precious Chinese medicinal material, and its main components are phenylethanol glycosides, polysaccharides and alkaloids. According to the Chinese Pharmacopoeia, Cistanche deserticola is warm in nature, sweet and salty in taste, and belongs to the kidney and large intestine meridians. It has the functions of replenishing kidney yang, benefiting essence and blood, and moistening the intestines and relieving constipation. It is mainly used to treat kidney yang deficiency, essence and blood deficiency, impotence and infertility, soreness of waist and knees, weakness of muscles and bones, dry intestines and constipation. Modern scientific research has proved that Cistanche deserticola has the functions of regulating immunity, anti-aging, anti-fatigue, protecting the liver, beautifying, and enhancing memory.

[0018] Ginger: It is the fresh rhizome of the perennial herbaceous plant ginger of the ginger family. Ginger tastes spicy and is slightly warm in nature. It enters the lung, spleen, and stomach meridians. It has the effects of dispelling cold, warming the middle and stopping vomiting, warming the lungs and stopping coughs, and detoxifying. It is often used for colds, spleen and stomach coldness, stomach cold vomiting, lung cold coughs, and detoxification of fish and crabs.

[0019] Cinnamon bark: It is the dried bark of the cinnamon bark of the Lauraceae family. It tastes pungent and sweet, and is very hot in nature. It enters the kidney, spleen, heart, and liver meridians. It has the effects of replenishing fire and yang, guiding fire back to the origin, dispersing cold and relieving pain, and warming and unblocking the meridians. It is used for impotence, cold uterus, cold pain in the waist and knees, asthma due to kidney deficiency, floating of false yang, dizziness and red eyes, cold pain in the heart and abdomen, vomiting and diarrhea due to deficiency and cold, abdominal pain due to cold hernia, and dysmenorrhea and amenorrhea.

[0020] Gorgon Fruit: It is a large annual aquatic herb of the Nymphaeaceae family. It has the effects of nourishing the kidney and consolidating essence, strengthening the spleen and stopping diarrhea, consolidating the kidney and consolidating essence, removing dampness and stopping leukorrhea, and is often used for spermatorrhea, enuresis, frequent urination, spleen deficiency, chronic diarrhea, leukorrhea, and leukorrhea.

[0021] Astragalus: The root of Astragalus is used as medicine, it tastes sweet and is slightly warm in nature. It has the effects of tonifying Qi and strengthening the exterior, diuretic, cardiotonic, lowering blood pressure, antibacterial, detoxifying, draining pus, promoting tissue regeneration, strengthening capillary resistance, antiperspirant and sex hormone-like. It can treat spontaneous sweating due to deficiency of the exterior, internal injury due to deficiency of Qi, diarrhea due to deficiency of the spleen, edema and carbuncle, etc.

[0022] Apple powder: It is a powdery substance extracted from apples. It is used in the fields of health care and beauty, and can prevent and protect some cardiovascular and cerebrovascular diseases.

[0023] Citrus fiber powder: It can promote intestinal peristalsis, lower blood sugar, control weight, lower cholesterol, increase saturation, etc. Citrus fiber is rich in polyphenols and has strong antioxidant ability, which can protect cells from damage by free radicals. At the same time, citrus fiber can also strengthen and prevent infection.

[0024] Lactic acid bacteria: organic acids, special enzymes, acid bacteria and other substances produced by fermentation have special physiological functions. A large amount of research data shows that lactic acid bacteria can promote animal growth, regulate normal gastrointestinal flora, maintain microecological balance, thereby improving gastrointestinal function, increasing food digestibility and biological efficacy, lowering serum cholesterol, controlling endotoxins, inhibiting the growth of putrefactive bacteria in the intestine, and improving the body's immunity.

[0025] Five-fingered peach: The main ingredients include organic acids, amino acids, triterpenes, alkaloids, coumarin, etc. It is often used in Lingnan area for spleen deficiency edema, loss of appetite, tuberculosis cough, night sweats, leukorrhea and other symptoms. It has the effects of strengthening the spleen and lungs, promoting qi and removing dampness, and beautifying the skin.

[0026] Poria: diuretic and dampness-removing medicine. Poria has multiple pharmacological effects such as diuresis, immunity enhancement, gastrointestinal function regulation, anti-tumor, liver protection, sedation, and antibacterial.

[0027] Job's tears: It is the dried mature kernel of the Gramineae plant Coix lachryma-jobi. It tastes sweet and light, is cool in nature, and enters the spleen, stomach, and lung meridians. Job's tears have the effects of promoting water and eliminating dampness, strengthening the spleen and stopping diarrhea, and removing numbness.

[0028] Imperata root: sweet in taste, cold in nature, has the effects of cooling blood and stopping bleeding, clearing away heat and promoting body fluid, and promoting diuresis and relieving stranguria. It is mainly used to treat blood heat bleeding, fever and thirst, lung heat and cough, dribbling and painful urination, edema, and jaundice.

[0029] Ginseng: It can resist shock, strengthen the heart, lower blood pressure, resist hypoxia and protect the myocardium, improve blood rheology and resist thrombosis; it can excite the pituitary-adrenal cortex system, resist stress, improve immune function, enhance hematopoietic function, regulate the balance of excitation and inhibition processes in the central nervous system, promote learning and memory, and resist fatigue; it can promote the synthesis of protein, RNA, and DNA; it can regulate cholesterol metabolism, lower blood lipids and blood sugar; it also has anti-inflammatory, anti-allergic, anti-diuretic, anti-tumor, anti-ulcer, anti-radiation, liver protection, and antioxidant effects.

[0030] Compared with the prior art, this application has the following beneficial effects:

[0031] 1. The nutrient powder has a significant dehumidifying effect, uses simple raw materials and has high business value;

[0032] 2. The preparation method of the nutrient powder adopts biological wall breaking technology, which allows the effective ingredients of Cistanche deserticola, ginseng, Imperata root, cinnamon and other components to dissolve quickly and maintain high activity; using microorganisms to destroy the cell walls of the components can better maintain the activity of various nutrients. It is carried out at room temperature, with low energy consumption, and can be industrialized;

[0033] 3. The nutritional powder fermented by lactic acid bacteria is more conducive to human absorption, and it can promote intestinal peristalsis together with citrus fiber powder;

[0034] 4. The added apple powder and citrus fiber powder not only provide vitamins, but also neutralize the bitterness of ginseng, making the powder taste better. DETAILED DESCRIPTION

[0035] For ease of understanding, the application will be described more fully below, and preferred embodiments of the application are provided. However, the application can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure of the present invention more thorough and comprehensive. In the embodiment, if specific techniques or conditions are not indicated, the techniques or conditions described in the document in this area or according to the product specification are carried out. Reagents used or instruments that do not indicate manufacturers are conventional products that can be obtained through the market.

[0036] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those generally understood by those skilled in the art of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0037] Example 1

[0038] (1) Biological cell wall breaking: ginger, cinnamon, female red date wine powder, coix seed, Euryale ferox, astragalus, longan, apple powder, citrus fiber powder, lactic acid bacteria, quinquefolius, Cistanche deserticola, Poria, Imperata root, and ginseng were weighed in a ratio of 0.8:0.8:7:0.8:0.7:1:1.2:1.2:1:2.8:1.7:1.2:1.5:0.6:1, the powders of each component except lactic acid bacteria were mixed according to the proportion, water was added, and yeast accounting for 12% of the total components was added for biological cell wall breaking for 2 hours to obtain a cell wall breaking mixture;

[0039] (2) fermentation: adding lactic acid bacteria to the cell wall-broken mixture, and fermenting at 25° C. for 18 h to obtain a fermentation mixture;

[0040] (3) Filtration and concentration: The fermentation mixture is adjusted to a pH of 6.5 and then filtered. The filtrate is concentrated and dried to obtain nutrient powder.

[0041] Example 2

[0042] (1) Biological cell wall breaking: ginger, cinnamon, female red date wine powder, coix seed, Euryale ferox, astragalus, longan, apple powder, citrus fiber powder, lactic acid bacteria, quinquefolius, Cistanche deserticola, Poria, Imperata root, and ginseng were weighed in a ratio of 1:1:8:1:0.8:1.2:1:1:0.7:3:2:1.5:2:0.7:1.3, and the powders of each component except lactic acid bacteria were mixed in proportion, and water was added, and yeast accounting for 15% of the total components was added for biological cell wall breaking for 3 hours to obtain a cell wall breaking mixture;

[0043] (2) Fermentation: adding lactic acid bacteria to the cell wall-broken mixture and fermenting at 28° C. for 24 h to obtain a fermentation mixture;

[0044] (3) Filtration and concentration: The fermentation mixture is adjusted to pH 7 and then filtered. The filtrate is concentrated and dried to obtain nutrient powder.

[0045] Example 3

[0046] (1) Biological cell wall breaking: ginger, cinnamon, female red date wine powder, coix seed, Euryale ferox, astragalus, longan, apple powder, citrus fiber powder, lactic acid bacteria, quinquefolius, Cistanche deserticola, Poria, Imperata root, and ginseng in a ratio of 0.5:2:5:0.4:0.5:0.5:1.5:1.5:1.2:2.5:1.5:1:1:0.5:08 were weighed, the powders of each component except lactic acid bacteria were mixed according to the proportion, water was added, and yeast with a total component of 10 was added for biological cell wall breaking for 2.5 hours to obtain a cell wall breaking mixture;

[0047] (2) fermentation: adding lactic acid bacteria to the cell wall-broken mixture, and fermenting at 22° C. for 12 h to obtain a fermentation mixture;

[0048] (3) Filtration and concentration: The fermentation mixture is adjusted to pH 8 and then filtered. The filtrate is concentrated and dried to obtain nutrient powder.

[0049] Comparative Example 1

[0050] Compared with Example 1, the difference is that no biological wall breaking technology is used.

[0051] (1) Biological cell wall breaking: ginger, cinnamon, female red date wine powder, coix seed, Euryale ferox, astragalus, longan, apple powder, citrus fiber powder, lactic acid bacteria, quinquefolius, Cistanche deserticola, Poria, Imperata root, and ginseng were weighed in a ratio of 0.8:0.8:7:0.8:0.7:1:1.2:1.2:1:2.8:1.7:1.2:1.5:0.6:1, and the powders of each component except lactic acid bacteria were mixed according to the proportion, and water was added to obtain a mixture;

[0052] (2) fermentation: adding lactic acid bacteria to the mixture and fermenting at 25° C. for 18 h to obtain a fermentation mixture;

[0053] (3) Filtration and concentration: The fermentation mixture is adjusted to a pH of 6.5 and then filtered. The filtrate is concentrated and dried to obtain nutrient powder.

[0054] Comparative Example 2

[0055] Compared with Example 1, the difference is that lactic acid bacteria fermentation is not used.

[0056] (1) Biological cell wall breaking: ginger, cinnamon, female red date wine powder, coix seed, Euryale ferox, astragalus, longan, apple powder, citrus fiber powder, water, quinquefolius, Cistanche deserticola, Poria, Imperata root, and ginseng were weighed in a ratio of 0.8:0.8:7:0.8:0.7:1:1.2:1.2:1:2.8:1.7:1.2:1.5:0.6:1, and the powders of each component except lactic acid bacteria were mixed according to the proportion, and water was added, and yeast accounting for 12% of the total components was added for biological cell wall breaking for 2 hours to obtain a cell wall breaking mixture;

[0057] (2) fermentation: adding weighed water to the cell-broken mixture, and fermenting at 25° C. for 18 h to obtain a fermentation mixture;

[0058] (3) Filtration and concentration: The fermentation mixture is adjusted to a pH of 6.5 and then filtered. The filtrate is concentrated and dried to obtain nutrient powder.

[0059] Comparative Example 3

[0060] Compared with Example 1, the difference lies in that Aspergillus niger is used.

[0061] (1) Biological cell wall breaking: ginger, cinnamon, female red date wine powder, coix seed, Euryale ferox, astragalus, longan, apple powder, citrus fiber powder, black Aspergillus niger, five-fingered peach, Cistanche deserticola, Poria, Imperata root, and ginseng in a ratio of 0.8:0.8:7:0.8:0.7:1:1.2:1.2:1:2.8:1.7:1.2:1.5:0.6:1 were weighed, the powders of each component except lactic acid bacteria were mixed according to the proportion, water was added, and yeast accounting for 12% of the total components was added for biological cell wall breaking for 2 hours to obtain a cell wall breaking mixture;

[0062] (2) fermentation: adding Aspergillus niger to the cell wall-broken mixture, and fermenting at 25° C. for 18 h to obtain a fermentation mixture;

[0063] (3) Filtration and concentration: The fermentation mixture is adjusted to a pH of 6.5 and then filtered. The filtrate is concentrated and dried to obtain nutrient powder.

[0064] Example 4

[0065] 1. Preparation of spleen deficiency model rats: The rats were fed with cabbage on odd days and gavaged with lard at a dose of 1.5 mL / 100 g on even days. At the same time, they were subjected to daily swimming to exhaustion. The mouth and nose of the rats were immersed in water, and the limbs and tail swung slowly until 5 bubbles came out of the mouth and nose at a time. The model was established for 14 consecutive days.

[0066] 2. Grouping and administration: 80 modeling rats were randomly divided into 8 groups, each with 10 rats, namely, model group, diuretic positive drug group, Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group, Comparative Example 2 group, and Comparative Example 3 group. Ten normal rats were randomly selected as the normal group, and the drug administration categories and dosages of each group were as follows: normal group and model group: purified water, 1 ml / 100 g; diuretic positive drug group: metolazone, 8 mg / kg / d; Example 1 group, Example 2 group, Example 3 group, Comparative Example 1 group, Comparative Example 2 group, and Comparative Example 3 group nutrient powder aqueous solution, 3000 mg / kg / d.

[0067] 3. Detection indicators

[0068] 3.1 Detection of urinary D-xylose excretion: After fasting for 12 hours, gavage with 3% D-xylose, 2mL / 100g, collect urine for 5 hours, and test according to the instructions of the D-xylose kit.

[0069] 3.2 Feces moisture content test: After collecting rat feces, dry them in an oven at 80℃ for 24 hours, take them out and let them cool, and measure the dry weight. After the measurement, put them in an oven at 80℃ and dry them for 1 hour, take them out and weigh them. If the difference between the two weights is no more than 3mg, the weight is considered constant. Feces moisture content (%) = (wet weight - dry weight) / wet weight × 100%.

[0070] 3.3 Blood biochemical index detection The rats in each group were fasted for 12 hours without water deprivation. Blood was collected from the ocular venous plexus of the animals and centrifuged at 3000r / min for 15min. The supernatant was aspirated and tested according to the instructions of the kit to determine total protein and albumin.

[0071] 3.4 Detection of Aquaporin 1 (AQP1) Prepare tissue homogenate from renal cord, add PBS at a ratio of 1:9, make homogenate in a tissue homogenizer, centrifuge at 3000 r / min for 20 min, aspirate the supernatant, store at -20°C, and determine AQP1 according to the operating steps in the kit manual.

[0072] 3.5 Serum gastrin (GAS) determination The rats in each group were fasted for 12 hours but not water, blood was collected from the ophthalmic venous plexus of the animals, centrifuged at 3000r / min for 15 minutes, and the supernatant was aspirated to determine serum GAS. The determination was carried out according to the operating steps in the kit instructions.

[0073] 3.6 Statistical methods SPSS17.0 software was used for statistics, and one-way analysis of variance was used for comparison. Normality and homogeneity of variance tests were performed first, and LSD test was used to detect whether both normality and homogeneity of variance were met.

[0074] 4. Test results:

[0075]

[0076] Note: Compared with the blank group, △△P<0.01; Compared with the model group, *P<0.05; Compared with the model group, **P<0.01.

[0077] From the above results, it can be seen that the water content in the feces of the mice in Example 1-3 groups increased significantly, while the change in the control group 1-3 was not much compared with the model group, indicating that the examples have a significant dehumidifying effect.

[0078] The above-mentioned embodiments only express the implementation methods of the present application, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the present application. It should be pointed out that, for a person of ordinary skill in the art, several modifications and improvements can be made without departing from the concept of the present application, and these all belong to the protection scope of the present application. Therefore, the protection scope of the present application shall be subject to the attached claims.

Claims

1. The composite dehumidifying nutrient powder prepared by biological wall breaking technology is characterized in that: The preparation method of the composite dehumidifying nutrient powder comprises the following steps: (1) Biological cell wall breaking: ginger, cinnamon, red date wine powder, coix seed, Euryale ferox, astragalus, longan, apple powder, citrus fiber powder, quinquefolius, cistanche deserticola, poria, Imperata root, and ginseng powder are mixed in proportion, water is added, and a microbial preparation is added for biological cell wall breaking to obtain a cell wall breaking mixture; (2) fermentation: adding lactic acid bacteria to the cell wall-broken mixture and fermenting to obtain a fermentation mixture; (3) Filtration and concentration: After adjusting the pH of the fermentation mixture, the mixture is filtered, and the filtrate is concentrated and dried to obtain nutrient powder; The components of the composite dehumidifying nutrient powder include: ginger, cinnamon, girls' red date wine powder, coix seed, euryale ferox, astragalus, longan, apple powder, citrus fiber powder, lactic acid bacteria, quinquefolius, cistanche, poria, white grass root, ginseng, and the mass ratio of each component is: 0.5-1:1-2:5-8:0.4-1:0.5-0.8:0.5-1.2:1-1.5:1-1.5:0.7-1.2:2.5-3:1.5-2:1-1.5:1-2:0.5-0.7:08-1.

3.

2. The composite dehumidifying nutrient powder prepared by the biological wall breaking technology according to claim 1, characterized in that: The biological cell wall breaking time in step (1) is 2-3 hours.

3. The composite dehumidifying nutrient powder prepared by the biological wall breaking technology according to claim 1, characterized in that: The microbial agent in step (1) is one or more of yeast, mold, and actinomycetes.

4. The composite dehumidifying nutrient powder prepared by the biological wall breaking technology according to claim 1, characterized in that: The amount of microbial agent used in step (1) is 10-15% of the total components.

5. The composite dehumidifying nutrient powder prepared by the biological wall breaking technology according to claim 1, characterized in that: In step (2), the fermentation temperature is 22-28° C. and the fermentation time is 12-24 hours.

6. The composite dehumidifying nutrient powder prepared by biological wall breaking technology according to claim 1, characterized in that: The pH value in the step (3) is 6-8.

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