Anti-dandruff composition as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of daily chemical products, and particularly relates to an anti-dandruff composition as well as a preparation method and application thereof. The anti-dandruff composition comprises a plant composition, piroctone olamine and a conditioner, the plant composition comprises at least one of aster, garden burnet, Chinese pulsatilla root, cockscomb and myrobalan and sarcandra glabra, and the conditioner comprises PCA zinc or myristicamidopropyl PG-dimethyl ammonium chloride phosphate. According to the plant composition disclosed by the invention, the plant composition and the chemical reagent are matched with each other while the plant composition plays a synergistic antibacterial and antioxidant role, so that the use amount of the chemical reagent can be effectively reduced through the synergistic effect, the antibacterial effect equivalent to that of the chemical reagent can be achieved, and the antioxidant capability of the chemical reagent is improved; the anti-dandruff composition can realize scientific anti-dandruff starting from an integral balance idea of'micro-ecology-barrier function-sebum secretion '.

Description

Technical Field

[0001] The invention belongs to the technical field of daily chemical products, and in particular relates to an anti-dandruff composition and a preparation method and application thereof. Background Art

[0002] Pityriasis capitis, commonly known as dandruff, is one of the most common skin diseases. More than half of adults worldwide are troubled by dandruff. Dandruff can cause itching and flaking of the skin, accompanied by mild inflammatory reactions. A normal scalp is like a balanced ecosystem. It is also a sensitive system that responds to any subtle changes. Therefore, the most important way to deal with scalp problems is to "find a balance". Dandruff is closely related to the imbalance of microecology, sebum secretion, and scalp barrier function. The physiological state of the human body, such as mental stress, eating habits, and hormonal changes during adolescence, can affect the secretion of sebum, and the secretion of scalp oil provides a rich source of nutrition for scalp microorganisms, especially Malassezia, which affect the colonization of microbial populations by changing the microenvironment of the scalp. The imbalance of scalp microorganisms, physiological state, and external stimuli can lead to oxidative stress, barrier dysfunction, and inflammatory reactions. Changes in the scalp barrier will directly affect the colonization of microorganisms, leading to changes in local microbial diversity. Therefore, if you want to remove dandruff scientifically and fundamentally and protect the overall health of your scalp, you must start from the overall balance of "microecology-barrier function-sebum secretion".

[0003] Commonly used chemical anti-dandruff agents on the market include salicylic acid, selenium disulfide, zinc pyrithione (ZPT), piroctone olamine (OCT), climbazole, and climbazole. Salicylic acid is a stratum corneum exfoliant, which does not have obvious antifungal effects, has poor anti-dandruff effects, is highly irritating, and can easily peel off the skin and cause damage; selenium disulfide is a cell growth inhibitor, which is highly irritating and will feel dry after use. Long-term residence will cause hair loss and odor; zinc pyrithione (ZPT, which has been banned in the European Union), piroctone olamine (OCT), climbazole, ketoconazole, etc. are all powerful fungicides, which have the disadvantages of being highly irritating and unfriendly to the environment. In addition, long-term use of chemical reagents will cause drug resistance and harm the immune system.

[0004] In recent years, with the popularity of environmental protection, low carbon and green trends, as well as the characteristics of plant raw materials with few side effects and low drug resistance, plant-derived anti-dandruff products have become a hot topic of research and are increasingly attracting people's attention and love. However, the efficacy of a single plant extract is often limited and it is difficult to meet the complex and changeable needs of the scalp. Therefore, compounding multiple plant extracts to achieve better anti-dandruff effects through the synergistic effect between the components, or compounding plant extracts with chemical reagents to achieve an effect equivalent to that of chemical reagents, can reduce the use of chemical reagents, improve the antioxidant effect of chemical reagents, and repair the scalp barrier.

[0005] For example, Chinese patent application CN202410536821.0 discloses a plant anti-dandruff composition, which is composed of the following components: lotus (NELUMBO NUCIFERA) extract, soybean extract, and licorice extract. The plant anti-dandruff composition can quickly remove dandruff, maintain the scalp in a healthy state for a long time, and is mild and low in irritation. However, the plant composition cannot achieve the same antibacterial effect as chemical reagents.

[0006] For another example, Chinese patent application CN202410172831.0 discloses a botanical antibacterial hair care nanoemulsion and its preparation method. The antibacterial components include: bergamot essential oil, cinnamon extract, lemon essential oil or Litsea cubeba essential oil, clove extract; cinnamon extract is cinnamon essential oil or cinnamaldehyde, and clove extract is clove essential oil or eugenol, which can effectively inhibit bacteria and remove dandruff, control water and oil balance, and nourish hair. However, cinnamaldehyde, Ceylon cinnamon bark oil, cinnamon leaf oil, citral, eugenol, clove (leaf, flower, stem, bud) oil and other related ingredients are all in the EU Cosmetics Regulation (EC) flavor and fragrance allergen catalog, which is more irritating and less mild, and may have a certain impact on the skin barrier, and cannot achieve a long-term anti-dandruff effect. Summary of the invention

[0007] The purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide an anti-dandruff composition based on the idea of ​​overall balance, and its preparation method and application. The composition contains chemical anti-dandruff agents, conditioners and plant compositions at the same time. Through synergistic effects, the amount of chemical reagents is reduced to achieve the purpose of all-round anti-dandruff of "microecology-barrier function-sebum secretion", and the components of the plant composition can also achieve synergistic antibacterial and antioxidant effects.

[0008] To achieve the above objectives, the technical solutions adopted by the present invention include:

[0009] In a first aspect, the present invention provides a synergistic antibacterial and antioxidant plant composition, the plant composition comprising a) and b):

[0010] a) at least one of Aster strychnifolia, Sanguisorba officinalis, Pulsatilla chinensis, Celosia cristata, and Terminalia chebula;

[0011] b) Coral grass;

[0012] The weight ratio of a):b) is (1-8):(1-2).

[0013] Coral contains isofraquinone and rosmarinic acid. Isofraquinone can inhibit the protein expression of COX-2 and reduce the levels of TNF-a, IL-6 and PGE2, thereby exerting its anti-inflammatory activity; rosmarinic acid is a natural antioxidant with strong antioxidant activity, which helps prevent cell damage caused by free radicals. Aster contains kaempferol, quercetin, umbelliferone, ferulic acid, etc. Quercetin treatment can enhance the communication between endothelial cells and hair follicle stem cells, while promoting the proliferation and migration of endothelial cells, thereby helping to activate hair follicle stem cells; in addition to its anti-inflammatory and antioxidant effects, ferulic acid also has the function of absorbing ultraviolet rays, which can prevent or reduce ultraviolet damage to the scalp barrier and hair. Sanguisorba officinalis mainly contains tannins, gallic acid, sanguisorba officinalis saponins and other ingredients. Tannins have good antibacterial and anti-allergic effects; gallic acid has the function of inhibiting COX-2 free radical scavenging. Pulsatilla mainly contains triterpenoid saponins and triterpenoid acids. Pulsatilla saponin B4 can significantly reduce the levels of inflammatory factors TNF-α, IL-1β, and IL-6. The main components of Celosia cristata are flavonoids such as quercetin, kaempferol, and luteolin, which have good antioxidant and anti-inflammatory properties. Terminalia chebula contains rich polyphenol compounds, such as flavonoids and tannins, which have antioxidant and anti-inflammatory effects, can help reduce scalp inflammation, and maintain scalp health; in addition, Terminalia chebula contains a certain amount of oil and amino acids, which can moisturize the scalp and reduce dry scalp and dandruff.

[0014] Through experimental research, it was found that when the present invention adopts b) coral grass and a) other plants in a) in a specific ratio, the components cooperate with each other, can play a good role in inhibiting the proliferation of Malassezia furfur and anti-oxidation, and achieve a strong synergistic effect, so that when it is applied to scalp care products, it can achieve a better anti-dandruff effect.

[0015] Preferably, the botanical composition comprises at least one of the following:

[0016] (1) The plant composition is composed of Sarcandra glabra and Aster strychnos nucifera, and the weight ratio of Sarcandra glabra and Aster strychnos nucifera is (1-2):(1-2);

[0017] (2) The plant composition comprises Sarcandra glabra, Aster truncatula and Sanguisorba officinalis, and the weight ratio of Sarcandra glabra, Aster truncatula and Sanguisorba officinalis is (1-2):1:(1-2);

[0018] (3) The plant composition is composed of Sarcandra glabra, Aster truncatula and Pulsatilla chinensis, and the weight ratio of Sarcandra glabra, Aster truncatula and Pulsatilla chinensis is (1-2):1:(1-2);

[0019] (4) The plant composition is composed of Sarcandra glabra, Aster truncatum and Celosia cristata, and the weight ratio of Sarcandra glabra, Aster truncatum and Celosia cristata is (1-2):1:(1-2);

[0020] (5) The plant composition is composed of Sarcandra glabra, Aster truncatula, Sanguisorba officinalis and Terminalia chebula, and the weight ratio of Sarcandra glabra, Aster truncatula, Sanguisorba officinalis and Terminalia chebula is (1-2):1:(1-2):(2-8).

[0021] Preferably, the plant composition is Sarcandra glabra, Aster truncatula and Sanguisorba officinalis, and the weight ratio of Sarcandra glabra, Aster truncatula and Sanguisorba officinalis is 1:1:1.

[0022] Experimental research found that when the three parties are compounded and Coral Grass, Aster and Sanguisorba officinalis are compounded in a ratio of 1:1:1, the synergistic antibacterial and antioxidant effects of the plant composition are best.

[0023] Preferably, the preparation method of the plant composition comprises the following steps: adding 8-12 times the amount of 70-80% ethanol or water to the plant raw material and soaking for 0.5-1.5h, then ultrasonic treatment, taking the filtrate and concentrating it to 0.8-1.2g / mL, centrifuging and taking the supernatant to obtain the plant composition.

[0024] In a second aspect, the present invention provides an anti-dandruff composition, which comprises the plant composition, piroctone olamine salt and a conditioner.

[0025] Preferably, the conditioning agent comprises zinc PCA or myristamidopropyl PG-dimonium chloride phosphate.

[0026] PCA zinc has the three-in-one effects of moisturizing, oil control, and photoaging resistance. It can reduce the activity of skin 5-α reductase, thereby reducing the excessive secretion of androgens, regulating the secretion of oil by sebaceous glands, inhibiting UVA-induced activation of activating protein-1 (AP-1), and significantly reducing the production of matrix metalloproteinase-1 (this mechanism is closely related to the slowing down of collagen degradation in photoaged skin). Myristamidopropyl PG-dimethylammonium chloride phosphate (PTM) as a surfactant can give hair a soft and shiny property and give the skin a smooth skin feel.

[0027] The present invention adopts the plant composition, the chemical anti-dandruff agent piroctone ethanolamine (OCT) and a conditioner to compound and prepare an anti-dandruff composition. While the plant composition exerts a synergistic antibacterial and antioxidant effect, the plant composition and the chemical reagent cooperate with each other, and the synergistic enhancement effect can effectively reduce the usage of the chemical reagent, and can achieve an antibacterial effect equivalent to that of the chemical reagent, and improve the antioxidant capacity of the chemical reagent, so that the anti-dandruff composition can achieve scientific dandruff removal based on the overall balance concept of "microecology-barrier function-sebum secretion".

[0028] Preferably, the conditioning agent is myristamidopropyl PG-dimethylammonium chloride phosphate, and the mass ratio of the plant composition, piroctone olamine salt and myristamidopropyl PG-dimethylammonium chloride phosphate is 2:2:1 or 10:2:1.

[0029] More preferably, the mass ratio of the plant composition, piroctone olamine salt and myristamidopropyl PG-dimethylammonium chloride phosphate is 2:2:1.

[0030] Preferably, the conditioning agent is PCA zinc, and the mass ratio of the plant composition, piroctone olamine salt and PCA zinc is (1-2):2:1.

[0031] More preferably, the mass ratio of the plant composition, piroctone olamine salt and PCA zinc is 1:2:1.

[0032] Preferably, the plant composition is Sarcandra glabra, Aster truncatula and Sanguisorba officinalis, and the mass ratio of Sarcandra glabra, Aster truncatula and Sanguisorba officinalis is 1:1:1.

[0033] After a large number of experiments and investigations, it was found that when the present invention uses the above-mentioned plant composition and OCT, PCA zinc or myristamidopropyl PG-dimethylammonium chloride phosphate to be scientifically compounded under a limited ratio, each component acts on the dandruff problem through multiple targets and dimensions, which can significantly enhance the antibacterial and antioxidant effects of the anti-dandruff composition, thereby achieving a scientific and long-lasting anti-dandruff effect based on the idea of ​​overall balance, and protecting the overall health of the scalp.

[0034] In a third aspect, the present invention provides a method for preparing the anti-dandruff composition, comprising the following steps:

[0035] S1. Add 8-12 times the amount of 70-80% ethanol or water to the plant raw material and soak for 0.5-1.5 hours, then ultrasonicate, concentrate the filtrate to 0.8-1.2 g / mL, centrifuge and take the supernatant to obtain the plant composition;

[0036] S2, dissolving piroctone olamine salt and PCA zinc with ethanol respectively, adding Tween to dissolve, to prepare piroctone olamine salt stock solution and PCA zinc stock solution; diluting myristyl amide propyl PG-dimethyl ammonium chloride phosphate with sterile enzyme-free water, to prepare myristyl amide propyl PG-dimethyl ammonium chloride phosphate stock solution;

[0037] S3, mixing the plant composition, piroctone olamine salt stock solution, PCA zinc stock solution or myristamidopropyl PG-dimethylammonium chloride phosphate stock solution to obtain the anti-dandruff composition.

[0038] In a fourth aspect, the present invention provides use of the plant composition or the anti-dandruff composition in preparing a scalp care product.

[0039] Compared with the prior art, the present invention has the following beneficial effects:

[0040] (1) When the present invention uses Coral Grass and at least one of the Aster scabra, Sanguisorba officinalis, Pulsatilla chinensis, Celosia cristata, and Terminalia chebula in a specific ratio for compound use, the components cooperate with each other to exert a good inhibitory effect on the proliferation of Malassezia furfur and an antioxidant effect, thereby achieving a strong synergistic effect, so that when it is used in a scalp care product, a better anti-dandruff effect can be achieved;

[0041] (2) The present invention uses the plant composition to compound with OCT and PCA zinc or PTM specific conditioning agents to prepare an anti-dandruff composition. While the plant composition exerts a synergistic antibacterial and antioxidant effect, the plant composition and the chemical reagents cooperate with each other. The synergistic enhancement effect can effectively reduce the amount of chemical reagents used, and can achieve an antibacterial effect equivalent to that of chemical reagents, and improve the antioxidant capacity of chemical reagents, so that the anti-dandruff composition can achieve scientific dandruff removal based on the overall balance concept of "microecology-barrier function-sebum secretion". BRIEF DESCRIPTION OF THE DRAWINGS

[0042] Figure 1 The figure is a test result of the scavenging ability of the plant composition representative obtained in Effect Example 2 (described in Example 1), OCT, PCA zinc, and PTM alone on DPPH free radicals;

[0043] Figure 2 The figure is a test result of the scavenging ability of the composition 4 (CZD) obtained in Effect Example 2 and its related formulas on DPPH free radicals;

[0044] Figure 3 The figure is a test result of the scavenging ability of the composition 1 (CZ) obtained in Effect Example 2 and its related formulas on DPPH free radicals;

[0045] Figure 4 The figure is a test result of the scavenging ability of the composition 7 (CZB) obtained in Effect Example 2 and its related formulas on DPPH free radicals;

[0046] Figure 5 This is a graph showing the test results of the scavenging ability of the composition 10 (CZJ) obtained in Effect Example 2 and its related formulations on DPPH free radicals.

[0047] Figure 6 This is a graph showing the test results of the scavenging ability of the composition 14 (CZDH) obtained in Effect Example 2 and its related formulations on DPPH free radicals. DETAILED DESCRIPTION

[0048] The following will be combined with the embodiments to clearly and completely describe the concept of the present invention and the technical effects produced, so as to fully understand the purpose, characteristics and effects of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of them. Based on the embodiments of the present invention, other embodiments obtained by technicians in this field without creative work are all within the scope of protection of the present invention. The test methods used in the embodiments are conventional methods unless otherwise specified; the materials, reagents, etc. used, unless otherwise specified, can be reagents and materials obtained from commercial channels. Unless otherwise specified, the same parameter in each embodiment has the same value. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limitations on the present invention.

[0049] Example 1

[0050] This embodiment provides a synergistic antibacterial and antioxidant plant composition, which comprises a) and b):

[0051] a) at least one of Aster strychnifolia, Sanguisorba officinalis, Pulsatilla chinensis, Celosia cristata, and Terminalia chebula;

[0052] b) Coral grass.

[0053] The synergistic compound composition is shown in Table 1.

[0054] Table 1 Plant synergy test formula

[0055]

[0056]

[0057] The preparation method of the plant composition comprises the following steps:

[0058] S1. Weigh 15g of each plant medicinal material, add 10 times the amount of 70% ethanol or water, soak for 1h, ultrasonically extract twice, each time for 20min, combine the two filtrates and concentrate to 1g / mL, centrifuge to obtain the plant supernatant, and store at 4℃ for later use;

[0059] S2. Dilute the total concentration of the plant supernatant obtained above to 200 mg / mL, without changing the total concentration, and adjust the proportion of each component according to Table 1 to obtain the plant composition 1-18.

[0060] Example 2

[0061] This embodiment provides a synergistic antibacterial and antioxidant anti-dandruff composition, including composition 4 (CZD) in Example 1, OCT, PCA zinc or myristamidopropyl PG-dimethylammonium chloride phosphate (PTM), and the formula of the anti-dandruff composition is shown in Table 2.

[0062] Table 2 Composition 4 (CZD), OCT, PCA zinc or PTM synergistic test formula

[0063]

[0064] The preparation method of the synergistic antibacterial and antioxidant anti-dandruff composition 19-26 comprises the following steps:

[0065] S1. Selecting the plant antibacterial synergistic composition with the best antibacterial effect among the three-party combination, composition 4, to screen the proportions of the plant composition, OCT, PCA zinc or PTM. Composition 4 (CZD) is prepared according to the method described in Example 1;

[0066] S2. Dissolve OCT and PCA zinc in ethanol (0.2% Tween as solubilizer) to prepare a stock solution with a concentration of 50 mg / mL; dilute myristic amidopropyl PG-dimethylammonium chloride phosphate (PTM) with sterile enzyme-free water to prepare a stock solution with a concentration of 200 mg / mL; seal the stock solution tube with a sealing film, and store it in a 4°C refrigerator away from light for future use;

[0067] S3. Mix composition 4 and the above stock solution to make the total concentration of composition 4 (CZD), OCT, PCA zinc or PTM synergistic test formula 50 mg / mL. Do not change the total concentration, adjust the proportion of each component according to Table 2, and prepare the anti-dandruff composition 19-26.

[0068] Example 3

[0069] This embodiment provides a synergistic antibacterial and antioxidant anti-dandruff composition, including other plant composition representatives in Example 1, OCT, PCA zinc or myristamidopropyl PG-dimethylammonium chloride phosphate (PTM), and the formula of the anti-dandruff composition is shown in Tables 3-5.

[0070] Table 3 Composition 1 (CZ), OCT, PCA zinc or PTM synergistic test formula

[0071]

[0072] Table 4 Composition 7 (CZB), OCT, PCA zinc or PTM synergistic test formula

[0073]

[0074] Table 5 Composition 10 (CZJ), OCT, PCA zinc or PTM synergistic test formula

[0075]

[0076] Table 6 Composition 14 (CZDH), OCT, PCA zinc or PTM synergistic test formula

[0077]

[0078] The preparation method of the above-mentioned synergistic antibacterial and antioxidant anti-dandruff composition 27-33 is the same as that of Example 2.

[0079] Application Example 1

[0080] In order to further verify the use effect of the anti-dandruff composition, composition 5 was added to shampoo products to conduct consumer research. The shampoo test formula is shown in Table 7.

[0081] Table 7 Shampoo formulation containing composition 5

[0082]

[0083]

[0084] The preparation process of the shampoo formula comprises the following steps: ① dispersing JR-400 with a certain amount of deionized water, stirring and heating until transparent, and setting aside; ② dispersing EDTA, ALS70, and ALES70 in the remaining deionized water, stirring until completely dissolved, adding item ①, and stirring until uniform; ③ heating to 75° C., adding CMEA, cetyl alcohol, and EGDS, stirring at the temperature until completely dissolved, and cooling; ④ cooling to 50° C., adding CHS; ⑤ cooling to 45° C., adding the remaining materials, stirring continuously for more than 30 minutes, observing the pearlescent condition of the material body, and appropriately extending the insulation time; ⑥ cooling to 38° C., sampling and inspection, and discharging the material after passing the test.

[0085] Subject screening: The subject information is obtained by asking about the situation in the past week, and the examiner will score the subject accordingly based on the subject's answer. Eligible subjects should meet the following conditions: have dandruff or are troubled by dandruff.

[0086] A total of 30 people participated in the consumer survey. The subjects used the test samples once a day or once every two days (depending on personal preference), and each shampooing time was no less than 2 minutes. After 28 days of use, the subjects were asked about their subjective feelings.

[0087] Regarding the degree of agreement about dandruff reduction, select: strongly agree, agree, disagree, and strongly disagree.

[0088] The degree of agreement on comfortable scalp: strongly agree, agree, disagree, strongly disagree.

[0089] The levels of agreement on overall satisfaction were: strongly agree, agree, disagree, and strongly disagree.

[0090] Effect Example 1

[0091] This test uses the microbroth dilution method to determine the minimum inhibitory concentration (MIC) of the positive drugs OCT, plant composition, PCA zinc, and PTM to evaluate the inhibitory effect of each test drug on Malassezia; the synergistic antibacterial effect of the plant composition, as well as the synergistic antibacterial effect between the plant composition and OCT, PCA zinc or PTM is evaluated by exploring the synergistic effect index (CI). The specific methods are as follows.

[0092] 1. Test Materials

[0093] Malassezia furfur (M. furfur, ATCC44344) was purchased from Shanghai Microorganism Collection Center, piroctone ethanolamine (OCT) was purchased from Tongmeiyuan (Guangdong) Biotechnology Co., Ltd., myristamidopropyl PG-dimethylammonium chloride phosphate (PTM for short) was purchased from Heda Chemicals (Shanghai) Co., Ltd., PCA zinc was purchased from Guangzhou Aichuang Biotechnology Co., Ltd., and coral grass, aster, sanguisorba officinalis, whitehead, cockscomb, and terminalia chebula were purchased from Qingping Market.

[0094] 2. Test Method

[0095] (1) Preparation of modified Dxion (mDixon) medium and modified Dxion (mDixon) agar medium: According to the instructions, accurately weigh the medium granules, agar powder and deionized water into a beaker, stir with a glass rod until the granules are completely dissolved, adjust the pH value to 6.1±0.1, dispense into conical bottles, seal, autoclave at 121°C for 30 min, cool and store in a refrigerator at 4°C for later use;

[0096] (2) Preparation of bacterial suspension: Take out the glycerol tube of the test bacteria stored in the -20°C refrigerator, use an inoculation loop to take 20 μL of the bacterial solution, inoculate it on the agar slant by streaking, place it in a 32°C constant temperature incubator and culture it for 2 days. Rinse the slant with physiological saline to obtain a high-concentration bacterial suspension; take another test tube, pour in an appropriate amount of raw salt, take an appropriate amount of high-concentration bacterial suspension so that its turbidity is consistent with that of a No. 5 McFarland turbidimetric tube, and obtain a final concentration of 1×10 6 CFU / mL bacterial suspension;

[0097] (3) Determination of MIC value:

[0098] ① Culture method: add 100 μL of modified Dixon (mDixon) liquid medium to the 2nd to 11th columns of the 96-well plate, add 200 μL of modified Dixon (mDixon) liquid medium to the 12th column, add 200 μL of each test drug to the 1st column, take 100 μL and dilute it horizontally to the 10th column (discard the excess 100 μL of drug solution), then take the bacterial suspension prepared in step (2) and add it to the 1st to 11th columns, 100 μL in each well; at this time, the final volume of each well is 200 μL, the 11th column is the growth control well, and the 12th column is the blank control well. Place the 96-well plate at 32°C and incubate for 1-2 days;

[0099] ②Evaluation criteria: The concentration of the drug solution in the sterile growth hole is the minimum inhibitory concentration (MIC) when observed with the naked eye;

[0100] (4) Synergistic antibacterial test and evaluation:

[0101] ① The Chou-Talalay model is a commonly used pharmacological model for evaluating the synergistic and antagonistic effects of a compound combination during the onset of action. The MIC of each example was measured according to the method described in (3), and the synergistic effect index (CI) was calculated using the Chou-Talalay formula to determine whether the compound combination was synergistic or not. The formula is as follows:

[0102]

[0103] ②Evaluation criteria: C a , C b , C c , C d are the concentrations corresponding to the antibacterial effects of components A, B, C, and D when acting alone; C A , C B , C C , C D It indicates the inhibitory effect observed at the corresponding concentration when each component is compounded into a composition, C A-D Result = Composition MIC experimental data × C A-D The proportion of a component in the composition; CI < 1 indicates a synergistic effect; CI = 1 indicates no interaction; CI > 1 indicates an antagonistic effect.

[0104] 3. Test results

[0105] (1) Table 8 shows the antibacterial results of a single drug:

[0106] Table 8 Single drug antibacterial results

[0107]

[0108] The antibacterial results of each single drug are shown in Table 8. All the above 9 single drugs have good antibacterial effects, and the MIC is ≤25 mg / mL, but none of them is equivalent to OCT. Therefore, based on the above results, the formula test was carried out, including the plant formula and the formula with OCT, to find out whether there is a synergistic plant formula or a synergistic formula with OCT to reduce the dosage of OCT.

[0109] (2) Table 9-26 shows the results of the synergistic antibacterial test of the plant composition described in Example 1:

[0110] Table 9 Synergistic test results of composition 1 (CZ) (mg / mL)

[0111]

[0112] Table 10 Synergistic test results of composition 2 (mg / mL)

[0113]

[0114] Table 11 Synergistic test results of composition 3 (mg / mL)

[0115]

[0116] Table 12 Composition 4 (CZD) synergistic test results (mg / mL)

[0117]

[0118] Table 13 Synergistic test results of composition 5 (mg / mL)

[0119]

[0120]

[0121] Table 14 Synergistic test results of composition 6 (mg / mL)

[0122]

[0123] Table 15 Synergistic test results of composition 7 (CZB) (mg / mL)

[0124]

[0125] Table 16 Synergistic test results of composition 8 (mg / mL)

[0126]

[0127] Table 17 Synergistic test results of composition 9 (mg / mL)

[0128]

[0129] Table 18 Synergistic test results of composition 10 (CZJ) (mg / mL)

[0130]

[0131] Table 19 Synergistic test results of composition 11 (mg / mL)

[0132]

[0133] Table 20 Synergistic test results of composition 12 (mg / mL)

[0134]

[0135]

[0136] Table 21 Synergistic test results of composition 13 (mg / mL)

[0137]

[0138] Table 22 Synergistic test results of composition 14 (mg / mL)

[0139]

[0140] Table 23 Synergistic test results of composition 15 (mg / mL)

[0141]

[0142] Table 24 Synergistic test results of composition 16 (mg / mL)

[0143]

[0144] Table 25 Synergistic test results of composition 17 (mg / mL)

[0145]

[0146] Table 26 Synergistic test results of composition 18 (mg / mL)

[0147]

[0148] Table 9-26 shows the results of the synergistic antibacterial test of the plant composition described in Example 1: ① In the two-way compound, when Coralliana: Aster = (1-2): 1, CI < 1, which has a synergistic antibacterial effect. ② In the three-way compound, when Coralliana: Aster: Sanguisorba officinalis = (1-2): 1: (1-2) and Coralliana: Aster: Pulsatilla striata / Celosia cristata = 1: 1: 1, CI < 1, which has a good synergistic antibacterial effect, among which Composition 4 (CZD, Coralliana: Aster: Sanguisorba officinalis = 1: 1: 1) is the best, with a MIC of 6.25 mg / mL; the MIC of Composition 4 is 1 / 2, 1 / 4, and 1 / 2 of the MIC of Coralliana, Aster, and Sanguisorba officinalis when used alone, respectively. In addition, the experiment also found that the plant combination and OCT have a synergistic antibacterial effect. When the composition 1 (CZ): OCT = 1: 1, CI = 0.5013, when the composition 4 (CZD): OCT = 1: 1, CI = 0.5025, the measured MIC is 31.25μg / mL, which is equivalent to OCT, and the amount of OCT is only half of the original to achieve the same effect. ③ In the four-way compound, when Coral Grass: Aster: Sanguisorba officinalis: Terminalia chebula = (1-2): 1: (1-2): (2-8), CI < 1, which has a synergistic antibacterial effect. The MIC of composition 14 is 2.604mg / mL, which is about 5 / 6, 5 / 24, 5 / 192, and <5 / 192 of the MIC of Terminalia chebula, Sanguisorba officinalis, Coral Grass, and Aster, respectively.

[0149] (3) Tables 27-34 show the synergistic antibacterial test results of the anti-dandruff composition prepared by composition 4 (CZD), OCT, PCA zinc or PTM described in Example 2:

[0150] Table 27 Synergistic test results of composition 19 (μg / mL)

[0151]

[0152] Table 28 Synergistic test results of composition 20 (μg / mL)

[0153]

[0154] Table 29 Synergistic test results of composition 21 (μg / mL)

[0155]

[0156]

[0157] Table 30 Synergistic test results of composition 22 (μg / mL)

[0158]

[0159] Table 31 Synergistic test results of composition 23 (μg / mL)

[0160]

[0161] Table 32 Synergistic test results of composition 24 (μg / mL)

[0162]

[0163]

[0164] Table 33 Synergistic test results of composition 25 (μg / mL)

[0165]

[0166] Table 34 Synergistic test results of composition 26 (μg / mL)

[0167]

[0168] The plant composition 4 (CZD) with the best antibacterial effect among the three-party combination was selected for synergistic testing with OCT, PCA zinc or PTM to screen the appropriate combination ratio. Tables 27-30 show the antibacterial results of different combinations of composition 4, OCT and PTM. The results show that composition 20 (composition 4: OCT: PTM = 2: 2: 1, CI = 0.8045, the amount of OCT is reduced to 80% of the original) and composition 22 (composition 4: OCT: PTM = 10: 2: 1, CI = 0.6312) have synergistic antibacterial effects. Tables 31-34 show the antibacterial results of different formulations of composition 4, OCT, and PCA zinc. The results show that composition 23 (composition 4: OCT: PCA zinc = 1: 2: 1, CI = 0.5169, MIC = 31.25 μg / mL, equivalent to the minimum inhibitory concentration of OCT, and the amount of OCT is only half of the original) and composition 24 (composition 4: OCT: PCA zinc = 2: 2: 1, CI = 0.829) have synergistic antibacterial effects. The synergistic antibacterial MIC ranking of the compositions is composition 23 < composition 20 (CI = 0.8045) = composition 24 (CI = 0.829) < composition 22.

[0169] According to the above results, the synergistic effect is best when the composition 4: OCT: PCA zinc = 1: 2: 1 and the plant composition 4: OCT: PTM = 2: 2: 1 in Example 2, and this ratio is used for subsequent synergistic formula testing.

[0170] (4) Tables 35-41 show the synergistic antibacterial test results of the anti-dandruff compositions prepared by other plant composition representatives, OCT, PCA zinc or PTM described in Example 3:

[0171] Table 35 Synergistic test results of composition 27 (μg / mL)

[0172]

[0173] Table 36 Synergistic test results of composition 28 (μg / mL)

[0174]

[0175] Table 37 Synergistic test results of composition 29 (μg / mL)

[0176]

[0177] Table 38 Synergistic test results of composition 30 (μg / mL)

[0178]

[0179] Table 39 Synergistic test results of composition 31 (μg / mL)

[0180]

[0181] Table 40 Synergistic test results of composition 32 (μg / mL)

[0182]

[0183] Table 41 Synergistic test results of composition 33 (μg / mL)

[0184]

[0185]

[0186] Tables 35-41 are the results of the synergistic antibacterial test of other plant compositions described in Example 3 (composition 1CZ, composition 7CZB, composition 10CZJ, composition 14CZDH), OCT, PCA zinc or PTM. The results show that the CI of the seven compositions are all <1, and all have good synergistic antibacterial effects.

[0187] Effect Example 2

[0188] This test evaluates the ability of single drugs and combination of drugs to resist oxidative damage by measuring their ability to scavenge DPPH free radicals.

[0189] 1. Test materials: 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) was purchased from Shanghai MacLean Biochemical Technology Co., Ltd.

[0190] 2. Test Method

[0191] The samples and VC were prepared into 20mg / ml, 2mg / ml, 200μg / mL, 20μg / mL, and 2μg / mL. 500μL of sample solutions of different concentrations were taken into plastic centrifuge tubes with a pipette, and 500μL of DPPH (0.2mmol / L, prepared in ethanol) solution was added. After shaking and avoiding light for 30 minutes, ethanol was used as a blank control and VC as a positive control. The absorbance was measured at 517nm, and three parallel experiments were performed; since it was found in the experiment that 2mg / ml OCT showed antioxidant activity, the antioxidant activity of each single drug and combination was compared at a concentration of 2mg / ml. The specific calculation formula is as follows:

[0192] DPPH clearance rate = (1-D1 / D2) × 100%

[0193] Note: D1 is the absorbance of the test sample or positive control; D2 is the absorbance of the blank control.

[0194] 3. Test results

[0195] (1) Table 42 and Figure 1 The test results of the scavenging ability of the plant composition described in Example 1 and OCT, PCA zinc, and PTM alone on DPPH free radicals are as follows:

[0196] Table 42 Test results of scavenging ability of DPPH free radicals alone

[0197]

[0198]

[0199] Note: CZDH means Sarcocorina: Aster: Sanguisorba officinalis: Terminalia chebula = 1:1:1:4, CZD means Sarcocorina: Aster: Sanguisorba officinalis = 1:1:1, CZB means Sarcocorina: Aster: Pulsatilla scabra = 1:1:1, CZ means Sarcocorina: Aster = 1:1, CZJ means Sarcocorina: Aster: Celosia cristata = 1:1:1. * indicates P < 0.05 compared with OCT, ** indicates P < 0.01, *** indicates P < 0.001, **** indicates P < 0.0001; # indicates P < 0.05 compared with VC, ## indicates P < 0.01, ### indicates P < 0.001, #### indicates P < 0.0001, ns indicates P > 0.05 with no significant difference.

[0200] Table 42 and Figure 1The antioxidant activity of the plant composition (described in Example 1), OCT, PCA zinc, and PTM alone. The results show that at a concentration of 2 mg / mL, the antioxidant activity of VC, composition 14 (CZDH), composition 4 (CZD), composition 7 (CZB), composition 1 (CZ), and composition 10 (CZJ) are all superior to that of OCT, and there are extremely significant differences (P < 0.0001, ****), which are 2.44 times (51.99%), 2.46 times (52.60%), 2.42 times (51.15%), 2.34 times (48.27%), 1.91 times (32.76%), and 1.63 times (22.76%) of the scavenging ability of OCT, respectively. Among them, the scavenging ability of composition 14 (CZDH) and composition 4 (CZD) on DPPH free radicals is equivalent to the antioxidant activity of VC at the same concentration, and there is no significant difference (P > 0.05). However, PCA zinc and PTM had almost no antioxidant activity.

[0201] (2) Table 43 and Figure 2 The test results of the scavenging ability of CZD-related prescriptions and other comparative prescriptions on DPPH free radicals shown in Example 2 are as follows:

[0202] Table 43 Scavenging rate of composition 4 and its related prescriptions on DPPH free radicals and comparison results

[0203]

[0204]

[0205] Note: * indicates P < 0.05 compared with OCT, ** indicates P < 0.01, *** indicates P < 0.001, **** indicates P < 0.0001. # indicates P < 0.05 compared with plant combination, OCT, PCA zinc or PTM formulation and plant combination, OCT formulation (without PCA zinc or PTM), ## indicates P < 0.01, ### indicates P < 0.001, #### indicates P < 0.0001, ns indicates P > 0.05 with no significant difference (the same below).

[0206] Table 43 and Figure 2The above are the antioxidant activity results of composition 4 and its related formulations. At a concentration of 2 mg / mL, CZD and the formulation described in Example 2 showed strong antioxidant effects, and were significantly superior to OCT (P < 0.0001). The antioxidant capacity was from large to small in the order of composition 4 (CZD) > composition 23 (CZD: OCT: PCA zinc (1: 2: 1)) > composition 20 (CZD: OCT: PTM (2: 2: 1)) > OCT. Composition 4 and composition 23 showed a scavenging effect similar to that of VC at the same concentration, with a scavenging rate of 87.18% and 83.51% for DPPH free radicals, which were 2.42 times (51.15% more) and 2.32 times (47.48% more) the scavenging capacity of OCT.

[0207] At a concentration of 2 mg / mL, PCA zinc and PTM had almost no antioxidant effect, but PCA zinc combined with CZD and OCT could significantly improve the antioxidant capacity. The scavenging ability of the combination 23 (CZD: OCT: PCA zinc (1:2:1)) and CZ: OCT (1:2) on DPPH free radicals was significantly different (P < 0.0001), and the scavenging rate was nearly twice that of CZ: OCT (1:2) (41.07% more). However, PTM had poor antibacterial and antioxidant effects on the CZD and OCT combinations.

[0208] (3) Tables 44-47 and Figure 3-6 The test results of the scavenging ability of other plant composition-related formulas and other comparative formulas on DPPH free radicals shown in Example 2 are as follows:

[0209] Table 44 Scavenging rate of composition 1 and its related prescriptions on DPPH free radicals and comparison results

[0210]

[0211]

[0212] Table 44 and Figure 3 The above are the antioxidant activity results of composition 1 and its related formulations. At a concentration of 2 mg / mL, composition 1 (CZ) and its related formulations described in Example 3 showed a strong antioxidant effect, composition 1 (CZ) > composition 27 (CZ: OCT: PTM (2:2:1)) > composition 28 (CZ: OCT: PCA zinc (1:2:1)) > OCT, and CZ and related formulations were significantly better than OCT (P < 0.0001).

[0213] At a concentration of 2 mg / mL, PCA zinc and PTM had almost no antioxidant effect. However, the addition of PCA zinc and PTM enhanced the antioxidant capacity, and there was a significant difference (P < 0.01). The scavenging capacity of CZ alone for DPPH reached 68.79, and the combination ratio of CZ and OCT was 1:1. The addition of PTM had the best effect on the antioxidant capacity of OCT, with an antioxidant capacity increase of 8.12%, and the scavenging of DPPH free radicals was nearly 50% (11.20% more than OCT).

[0214] Table 45 Scavenging rate of composition 7 and its related prescriptions on DPPH free radicals and comparison results

[0215]

[0216]

[0217] Table 45 and Figure 4 The above are the antioxidant activity results of composition 7 and its related formulations. At a concentration of 2 mg / mL, composition 7 (CZB) and its related formulation described in Example 3 showed a strong antioxidant effect, and their antioxidant capacity from large to small were composition 7 (CZB) > composition 30 (CZB: OCT: PCA zinc (1:2:1)) > composition 29 (CZB: OCT: PTM (2:2:1)) > OCT, and the antioxidant effect of CZ and related formulations was significantly different from that of OCT (P < 0.001); among them, CZB had the best antioxidant effect, and the scavenging rate of DPPH free radicals was similar to that of VC at the same concentration, reaching 84.30%.

[0218] At a concentration of 2 mg / mL, PCA zinc and PTM had almost no antioxidant effect. The antioxidant activity of adding PCA zinc and PTM was greater than that of the combination of CZB and OCT alone, and there was a significant difference (P < 0.0001). The scavenging rates of composition 30 (CZB:OCT:PCA zinc (1:2:1)) and composition 29 (CZB:OCT:PTM (2:2:1)) on DPPH radicals reached 56.92% and 49.24%, which were 1.56 times (20.49% more) and 1.35 times (12.81% more) of the scavenging rate of OCT, respectively, and 1.55 times (20.10% more) and 1.23 times (9.05% more) of CZB:OCT (1:1) and CZB:OCT (1:2), respectively.

[0219] Table 46 Scavenging rate of composition 10 and its related prescriptions on DPPH free radicals and comparison results

[0220]

[0221]

[0222] Table 46 and Figure 5 The above are the antioxidant activity results of composition 10 and its related formulations. At a concentration of 2 mg / mL, the antioxidant activity of CZJ is not as good as that of CZ, CZD, CZB, and CZDH, but CZJ and its related formulations also show antioxidant effects that are superior to OCT. The antioxidant capacity is from large to small, composition 10 (CZJ) > composition 31 (CZJ: OCT: PTM (2:2:1)) > composition 32 (CZJ: OCT: PCA zinc (1:2:1)), and is significantly superior to OCT (P < 0.001). However, the addition of PCA zinc and PTM has poor antioxidant synergistic effect on the CZJ and OCT formulations. Only PCA zinc has a significant improvement, but the scavenging rate of DPPH free radicals is only increased by 3.78%.

[0223] Table 47 Scavenging rate of composition 14 and its related prescriptions on DPPH free radicals and comparison results

[0224]

[0225] Table 47 and Figure 6 The above are the antioxidant activity results of composition 14 and its related formulas. At a concentration of 2 mg / mL, OCT has poor antioxidant activity and PCA zinc has almost no antioxidant activity. However, CZDH can significantly improve the antioxidant activity of OCT and PCA zinc after compounding with OCT and PCA zinc. The antioxidant effect of the combination is equivalent to that of VC, and there is a very significant difference with OCT and PCA zinc (P < 0.0001). The scavenging ability after the combination is 2.43-2.46 times that of OCT (51.52%-52.60% more) and 110.82-112.19 times that of PCA zinc (86.76%-87.84% more).

[0226] Effect Example 3

[0227] The consumer survey results of the shampoo formula containing composition 5 are shown in Table 48.

[0228] Table 48 Consumer survey results of shampoo formula containing composition 5

[0229]

[0230] According to the data in Table 48, the shampoo containing composition 5 has reached more than 80% in the indexes of recognition of reducing dandruff, recognition of comfortable scalp, and recognition of overall satisfaction. The base of the shampoo does not contain anti-dandruff ingredients, and the only anti-dandruff active ingredient is composition 5. It can be seen that composition 5 can reduce dandruff according to consumer research.

[0231] Finally, it should be noted that the above embodiments are intended to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A synergistic antibacterial and antioxidant plant composition, characterized in that: The botanical composition comprises a) and b): a) at least one of Aster strychnifolia, Sanguisorba officinalis, Pulsatilla chinensis, Celosia cristata, and Terminalia chebula; b) Coral grass; The weight ratio of a):b) is (1-8):(1-2).

2. The plant composition according to claim 1, characterized in that The plant composition comprises at least one of the following: (1) The plant composition is composed of Sarcandra glabra and Aster strychnos nucifera, and the weight ratio of Sarcandra glabra and Aster strychnos nucifera is (1-2):(1-2); (2) The plant composition comprises Sarcandra glabra, Aster truncatula and Sanguisorba officinalis, and the weight ratio of Sarcandra glabra, Aster truncatula and Sanguisorba officinalis is (1-2):1:(1-2); (3) The plant composition is composed of Sarcandra glabra, Aster truncatula and Pulsatilla chinensis, and the weight ratio of Sarcandra glabra, Aster truncatula and Pulsatilla chinensis is (1-2):1:(1-2); (4) The plant composition is composed of Sarcandra glabra, Aster truncatum and Celosia cristata, and the weight ratio of Sarcandra glabra, Aster truncatum and Celosia cristata is (1-2):1:(1-2); (5) The plant composition is composed of Sarcandra glabra, Aster truncatula, Sanguisorba officinalis and Terminalia chebula, and the weight ratio of Sarcandra glabra, Aster truncatula, Sanguisorba officinalis and Terminalia chebula is (1-2):1:(1-2):(2-8).

3. The plant composition according to claim 2, characterized in that The plant composition comprises Sarcandra glabra, Aster truncatula and Sanguisorba officinalis, and the weight ratio of Sarcandra glabra, Aster truncatula and Sanguisorba officinalis is 1:1:

1.

4. The plant composition according to any one of claims 1 to 3, characterized in that The preparation method of the plant composition comprises the following steps: Add 8-12 times the amount of 70-80% ethanol or water to the plant raw material and soak for 0.5-1.5 hours, then perform ultrasonic treatment, concentrate the filtrate to 0.8-1.2 g / mL, centrifuge and take the supernatant to obtain the plant composition.

5. An anti-dandruff composition, characterized in that: The anti-dandruff composition comprises the plant composition according to any one of claims 1 to 4; the anti-dandruff composition further comprises piroctone olamine salt and a conditioner.

6. The anti-dandruff composition according to claim 5, characterized in that Such conditioning agents include zinc PCA or myristamidopropyl PG-dimonium chloride phosphate.

7. The anti-dandruff composition according to claim 6, characterized in that The conditioning agent is myristamidopropyl PG-dimethylammonium chloride phosphate, and the mass ratio of the plant composition, piroctone olamine salt and myristamidopropyl PG-dimethylammonium chloride phosphate is 2:2:1 or 10:2:

1.

8. The anti-dandruff composition according to claim 6, characterized in that The conditioning agent is PCA zinc, and the mass ratio of the plant composition, piroctone olamine salt and PCA zinc is (1-2):2:

1.

9. The method for preparing the anti-dandruff composition according to any one of claims 5 to 8, characterized in that: The following steps are involved: S1. Add 8-12 times the amount of 70-80% ethanol or water to the plant raw material and soak for 0.5-1.5 hours, then ultrasonicate, concentrate the filtrate to 0.8-1.2 g / mL, centrifuge and take the supernatant to obtain the plant composition; S2, dissolving piroctone olamine salt and PCA zinc with ethanol respectively, adding Tween to dissolve, to prepare piroctone olamine salt stock solution and PCA zinc stock solution; diluting myristyl amide propyl PG-dimethyl ammonium chloride phosphate with sterile enzyme-free water, to prepare myristyl amide propyl PG-dimethyl ammonium chloride phosphate stock solution; S3, mixing the plant composition, piroctone olamine salt stock solution, PCA zinc stock solution or myristamidopropyl PG-dimethylammonium chloride phosphate stock solution to obtain the anti-dandruff composition.

10. Use of the plant composition according to any one of claims 1 to 4 or the anti-dandruff composition according to any one of claims 5 to 8 in the preparation of scalp care products.

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