Preparation method of traditional Chinese medicine plant extraction fermentation liquor

Abstract

The invention relates to the technical field of traditional Chinese medicine fermentation liquor, and particularly discloses a preparation method of traditional Chinese medicine plant extraction fermentation liquor, which comprises the following steps: carrying out enzymolysis on traditional Chinese medicinal materials with cellulase and other enzymes, adding water, decocting, and carrying out secondary fermentation on the obtained liquid medicine, so that active ingredients in the medicinal materials are fully released; after the fermentation liquor is sterilized and inactivated, sodium benzoate and propyl p-hydroxybenzoate are added for corrosion prevention, and the traditional Chinese medicine plant extraction fermentation liquor with the effects of maintaining beauty and keeping young is obtained.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicine fermentation liquid, and in particular to a method for preparing traditional Chinese medicine plant extract fermentation liquid. Background Art

[0002] Having a beautiful face has been the pursuit of many people since ancient times. In ancient my country, people have begun to use acupuncture, massage, and taking Chinese medicine to beautify themselves. Among them, Chinese medicine beauty methods are the most abundant, and many prescriptions have the effects of promoting blood circulation, moisturizing the skin, and beautifying the skin. Chinese medicine fermentation refers to the use of microorganisms to process Chinese medicinal materials and Chinese medicinal material extracts. Through fermentation technology, the plant cell walls of Chinese medicinal materials can be destroyed, so that their effective ingredients can be fully released, the utilization rate of Chinese medicine can be improved, and toxic substances can be decomposed into low-toxic or non-toxic substances to reduce their side effects. By applying Chinese medicine fermentation technology, people have achieved good results in medical care, animal husbandry, cosmetics and other industries. However, the current beauty Chinese medicine plant extract fermentation liquid still has problems such as insufficient fermentation, a lot of impurities precipitation, and easy deterioration of fermented Chinese medicine liquid. Summary of the invention

[0003] The purpose of the present invention is to provide a method for preparing a Chinese medicinal plant extract fermentation liquid, so as to solve the problems that the Chinese medicinal plant extract fermentation liquid is not fully fermented, there are many impurities precipitated, and the fermented Chinese medicinal liquid is easy to deteriorate.

[0004] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0005] A method for preparing a Chinese medicinal plant extract fermentation liquid, the preparation method comprising:

[0006] Step 1: Mix the Chinese medicine composition and deionized water, adjust the pH to 4.5-5.5 with food-grade acetic acid, add the medicinal complex enzyme, stir evenly, and perform enzymolysis at 35-40° C. for 10-14 hours. During the enzymolysis process, stir once every 3-5 hours, and stir for 8-12 minutes each time. After the enzymolysis is completed, boil the mixed system over high heat, and then simmer over low heat. After simmering for 80-100 minutes, filter out the filtrate, add deionized water to the filter residue, boil it over high heat again, simmer over low heat for 50-70 minutes, and filter out the filtrate. Mix the filtrates from the two filtrations, and vacuum concentrate at 55-65° C. to obtain a Chinese medicine enzymolysis extract;

[0007] Step 2: sterilize the Chinese medicine enzymatic hydrolysis extract with steam at a steam temperature of 125 to 130° C. and a steam pressure of 0.1 to 0.15 MPa for 20 to 40 minutes to obtain a sterilized Chinese medicine extract;

[0008] Step 3: inoculating the sterilized Chinese medicine extract with mixed yeast, aerobically fermenting at 28-32° C. for 6-8 days, and performing steam sterilization after the aerobic fermentation, with the steam temperature being 125-130° C. and the steam pressure being 0.1-0.15 MPa for 20-40 minutes to obtain a primary fermentation Chinese medicine extract;

[0009] Step 4: inoculating the primary fermented Chinese medicine extract with secondary anaerobic fermentation bacteria, anaerobically fermenting at 35-40° C. for 13-17 days, and steam sterilizing after the anaerobic fermentation, with the steam temperature at 125-130° C. and the steam pressure at 0.1-0.15 MPa for 20-40 minutes to obtain a secondary fermented Chinese medicine liquid;

[0010] Step 5: Mix the secondary fermented Chinese medicine liquid, sodium benzoate and propyl parahydroxybenzoate, stir evenly, filter out the filtrate, fill and seal for storage, and obtain the Chinese medicine plant extract fermentation liquid.

[0011] As a limitation of the present invention, the Chinese medicine composition includes, by mass, 13-17 parts of Bletilla striata, 28-32 parts of Lilium, 16-20 parts of Clove, 8-12 parts of Apricot, 23-27 parts of Taraxacum, 23-27 parts of Salvia miltiorrhiza, 17-21 parts of Tribulus terrestris, 11-15 parts of Bambusa obtusifolia, 13-17 parts of White Peony Root, 13-17 parts of Lemon, 3-7 parts of Gleditsia sinensis, 13-17 parts of White Poria, 4-8 parts of Mung Bean, 1-5 parts of Ginkgo, 17-21 parts of Perilla frutescens, 9-13 parts of Leonurus japonicus, and 4-8 parts of Tangerine Peel.

[0012] As a limitation of the present invention, the medicinal complex enzyme includes 5 to 7 parts of cellulase, 1 to 3 parts of hemicellulase, and 1 to 3 parts of pectinase by weight.

[0013] As a limitation of the present invention, the mass ratio of the Chinese medicine composition, deionized water and complex enzyme is (95-105):(290-310):(0.18-0.22), and the mass of deionized water added during the two decoctions is equal.

[0014] As a limitation of the present invention, the mixed yeast comprises 0.8 to 1.2 parts of saccharomyces cerevisiae, 0.8 to 1.2 parts of baker's yeast, and 1.8 to 2.2 parts of Candida.

[0015] As a limitation of the present invention, the mass of the inoculated mixed yeast is 1-3% of the mass of the sterilized traditional Chinese medicine extract.

[0016] As a limitation of the present invention, the secondary anaerobic fermentation bacteria include 0.8 to 1.2 parts of Bifidobacterium lactis, 1.8 to 2.2 parts of Streptococcus lactis, 0.8 to 1.2 parts of Bifidobacterium longum, and 0.8 to 1.2 parts of Lactobacillus plantarum.

[0017] As a limitation of the present invention, the mass of the inoculated secondary anaerobic fermentation bacteria is 4-5% of the mass of the primary fermentation Chinese medicine extract.

[0018] As a limitation of the present invention, the mass ratio of the secondary Chinese medicine fermentation broth, sodium benzoate and propyl paraben is (97-99):(1.4-1.6):(0.4-0.6).

[0019] Pharmacological analysis:

[0020] Bletilla striata is cool in nature, bitter, sweet and astringent in taste, and enters the lung, liver and stomach meridians. It is effective in treating chapped hands and feet, burning pain from ulcers, burns from hot water or fire, and carbuncles and swelling. "Materia Medica" records: Bletilla striata can treat facial blisters and make the skin smooth.

[0021] Lily is mild in nature and sweet in taste. It has the effects of suppressing cough, removing phlegm, calming the nerves and moistening the lungs. "Compendium of Materia Medica" records that applying raw lily to the affected area can cure pemphigus.

[0022] Clove is warm in nature and spicy in taste. It enters the spleen and stomach meridians and is effective in treating abdominal distension, nausea, diarrhea, and toothache.

[0023] Almonds are warm in nature and sweet in taste. They belong to the lung and large intestine meridians. They have the effects of digesting food and detoxifying, dispersing wind and cold, and relieving cough and asthma.

[0024] Dandelion is mild in nature and sweet in taste. It has the effects of relieving bloating and detoxifying, clearing away heat and dampness, and relieving swelling and dispersing nodules. "Compendium of Materia Medica" records: Mashing dandelion for application, and decocting the juice with wine can eliminate malnutrition and carbuncle.

[0025] Salvia miltiorrhiza is slightly cold in nature, bitter in taste, and enters the heart and liver meridians. It has the effects of promoting blood circulation and removing blood stasis, calming the mind, clearing the heart and relieving restlessness.

[0026] Tribulus terrestris is warm in nature and bitter in taste. It enters the lung, liver, and kidney meridians. It is effective in treating itching caused by urticaria, red and tearful eyes, and hyperactivity of liver fire.

[0027] Bamboo leaves are cold in nature and sweet in taste. They have the effects of clearing away heat and relieving restlessness, promoting fluid production and promoting urination, and eliminating phlegm and relieving cough.

[0028] White peony root is slightly cold in nature, bitter and sour in taste, and enters the liver and spleen meridians. It has the effects of antibacterial and analgesic, calming liver yang, nourishing blood and nourishing yin.

[0029] Lemon is mild in nature and sour in taste. It has the effects of quenching thirst, promoting salivation, relieving stagnation and strengthening the stomach, and relieving pain.

[0030] Soap beans are warm in nature, spicy and salty in taste, and have the effects of dispelling wind and resolving phlegm, reducing swelling, opening the orifices, breaking up tumors and killing insects.

[0031] White Poria cocos is mild in nature and sweet in taste. It has the effects of diuresis and dampness elimination, invigorating the spleen and calming the mind, removing spots and whitening the skin.

[0032] Mung beans are cold in nature and sweet in taste. They have the effects of detoxification, reducing swelling and stopping diarrhea. "Compendium of Materia Medica" records: Use two taels of mung bean powder and one tael of talcum powder, mix well and apply to the affected area to relieve summer heat rash.

[0033] Ginkgo nuts are mild in nature, sweet, bitter and astringent in taste, and belong to the lung meridian. They have the effects of resolving phlegm and relieving cough, replenishing Qi and nourishing the heart, promoting muscle growth, eliminating sores and detoxifying. "Compendium of Materia Medica" records: Ginkgo nuts can be crushed and applied to the nose, face, hands and feet to remove chapped skin, dark circles, wrinkles, scabies and malnutrition.

[0034] White perilla is warm in nature and pungent in taste. It has the effects of dispelling cold, relieving exterior symptoms, regulating qi and relieving fullness.

[0035] Motherwort is mild in nature and sweet in taste. It can be applied externally on the face to treat dark skin, remove facial spots and wrinkles. The "Motherwort Zemian Recipe" is made with motherwort as the main ingredient.

[0036] Tangerine peel is warm in nature and spicy in taste. It belongs to the lung meridian and has the effects of lowering qi, relieving cough, stopping diarrhea and promoting diuresis.

[0037] Compared with the prior art, the present invention has the following beneficial effects:

[0038] The invention adopts a variety of plant Chinese medicinal materials as raw materials, combines their medicinal properties to make it, and has the effects of whitening, moisturizing, removing spots and acne, and removing wrinkles.

[0039] The invention uses enzyme to treat Chinese medicine, so that the effective ingredients in the Chinese medicine can be fully released into the medicinal liquid, thereby improving the utilization rate of the Chinese medicine, using a secondary fermentation method to make the effective ingredients in the decocted medicinal liquid easier to be absorbed by the human body, and reducing the toxicity of some medicinal materials and reducing side effects. After fermentation, high-temperature steam sterilization is used and sodium benzoate and propyl paraben are added for preservation, thereby reducing the possibility of deterioration of the fermentation liquid, extending the shelf life of the fermentation liquid, and improving the quality of the fermentation liquid. DETAILED DESCRIPTION

[0040] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0041] Chinese medicinal materials such as Bletilla striata, lily, and clove were all from Beijing Tong Ren Tang, cellulase (≥0.3 units / mg), hemicellulase (0.3-3.0 units / mg), and pectinase (≥5 units / mg) were all from Shanghai Merck, and fermentation bacteria such as brewer's yeast and baker's yeast were all from Shaanxi Institute of Biological Agriculture.

[0042] Example 1: A method for preparing a Chinese medicinal plant extract fermentation liquid, specifically:

[0043] Step 1: Preparation of Chinese herbal medicine enzymatic extract

[0044] By weight, 15 parts of Bletilla striata, 30 parts of Lilium, 18 parts of Clove, 10 parts of Apricot, 25 parts of Dandelion, 25 parts of Salvia miltiorrhiza, 19 parts of Tribulus terrestris, 13 parts of Bamboo Leaf, 15 parts of White Peony Root, 15 parts of Lemon, 5 parts of Gleditsia sinensis, 15 parts of White Poria, 6 parts of Mung Bean, 3 parts of Ginkgo, 19 parts of Perilla frutescens, 11 parts of Leonurus japonicus, 6 parts of Orange Peel and 750 parts of deionized water were mixed, the pH was adjusted to 5 with food grade acetic acid, and 0.3 parts of cellulase, 0.1 parts of hemicellulase, 0.1 parts of cellulose and 0.1 parts of cellulose were added. parts of pectinase, stir evenly, and enzymolyze at 37°C for 12 hours. During the enzymolysis process, stir once every 4 hours, and stir for 10 minutes each time. After the enzymolysis is completed, boil the mixed system over high heat, and then simmer over low heat. After simmering for 90 minutes, filter out the filtrate. Add 750 parts of ionized water to the residue and boil it again over high heat. Simmer over low heat for 60 minutes and filter out the filtrate. Mix the filtrates from the two filtrations and concentrate in vacuum at 60°C until 500 parts of the filtrate remain to obtain the Chinese medicine enzymatic extract.

[0045] Step 2: Preparation of sterilized Chinese herbal medicine extract

[0046] The Chinese medicine enzymatic hydrolysis extract was sterilized by steam at a steam temperature of 127° C. and a steam pressure of 0.125 MPa for 30 minutes to obtain a sterilized Chinese medicine extract.

[0047] Step 3: Prepare primary fermentation Chinese herbal medicine extract

[0048] By mass, 1 part of brewer's yeast, 1 part of baker's yeast, and 2 parts of Candida were mixed to obtain a mixed yeast. 100 parts of sterilized traditional Chinese medicine extract were inoculated with 2 parts of the mixed yeast and aerobically fermented at 30°C for 7 days. After the aerobic fermentation, steam sterilization was carried out with a steam temperature of 127°C and a steam pressure of 0.125MPa for 30 minutes to obtain a once-fermented traditional Chinese medicine extract.

[0049] Step 4: Prepare the secondary fermentation Chinese medicine solution

[0050] By mass, 1 part of Bifidobacterium lactis, 2 parts of Streptococcus lactis, 1 part of Bifidobacterium longum, and 2 parts of Lactobacillus plantarum were mixed to obtain secondary anaerobic fermentation bacteria, 100 parts of primary fermentation Chinese medicine extract were inoculated with 4.5 parts of secondary anaerobic fermentation bacteria, and anaerobically fermented at 37°C for 15 days. After the anaerobic fermentation, steam sterilization was performed with a steam temperature of 127°C and a steam pressure of 0.125 MPa for 30 minutes to obtain a secondary fermentation Chinese medicine liquid.

[0051] Step 5: Prepare the fermented liquid of Chinese herbal medicine extract

[0052] 98 parts of secondary fermented Chinese medicine liquid, 1.5 parts of sodium benzoate and 0.5 parts of propyl p-hydroxybenzoate were mixed by mass, stirred evenly, the filtrate was filtered out, and the liquid was sealed and stored to obtain the Chinese medicine plant extract fermentation liquid.

[0053] Embodiment 2: A method for preparing a Chinese medicinal plant extract fermentation liquid, specifically comprising:

[0054] Step 1: Preparation of Chinese herbal medicine enzymatic extract

[0055] By weight, 15 parts of Bletilla striata, 30 parts of Lilium, 18 parts of Clove, 10 parts of Apricot, 25 parts of Dandelion, 25 parts of Salvia miltiorrhiza, 19 parts of Tribulus terrestris, 13 parts of Bamboo Leaf, 15 parts of White Peony Root, 15 parts of Lemon, 5 parts of Gleditsia sinensis, 15 parts of White Poria, 6 parts of Mung Bean, 3 parts of Ginkgo, 19 parts of Perilla frutescens, 11 parts of Leonurus japonicus, 6 parts of Orange Peel and 750 parts of deionized water were mixed, the pH was adjusted to 5 with food grade acetic acid, and 0.3 parts of cellulase, 0.1 parts of hemicellulase, 0.1 parts of cellulose and 0.1 parts of cellulose were added. parts of pectinase, stir evenly, and enzymolyze at 37°C for 12 hours. During the enzymolysis process, stir once every 4 hours, and stir for 10 minutes each time. After the enzymolysis is completed, boil the mixed system over high heat, and then simmer over low heat. After simmering for 90 minutes, filter out the filtrate. Add 750 parts of ionized water to the residue and boil it again over high heat. Simmer over low heat for 60 minutes and filter out the filtrate. Mix the filtrates from the two filtrations and concentrate in vacuum at 60°C until 500 parts of the filtrate remain to obtain the Chinese medicine enzymatic extract.

[0056] Step 2: Preparation of sterilized Chinese herbal medicine extract

[0057] The Chinese medicine enzymatic hydrolysis extract was sterilized by steam at a steam temperature of 127° C. and a steam pressure of 0.125 MPa for 30 minutes to obtain a sterilized Chinese medicine extract.

[0058] Step 3: Prepare primary fermentation Chinese herbal medicine extract

[0059] By mass, 1 part of brewer's yeast, 1 part of baker's yeast, and 2 parts of Candida were mixed to obtain a mixed yeast. 100 parts of sterilized traditional Chinese medicine extract were inoculated with 1 part of the mixed yeast and aerobically fermented at 30°C for 7 days. After the aerobic fermentation, steam sterilization was carried out with a steam temperature of 127°C and a steam pressure of 0.125MPa for 30 minutes to obtain a once-fermented traditional Chinese medicine extract.

[0060] Step 4: Prepare the secondary fermentation Chinese medicine solution

[0061] By mass, 1 part of Bifidobacterium lactis, 2 parts of Streptococcus lactis, 1 part of Bifidobacterium longum, and 2 parts of Lactobacillus plantarum were mixed to obtain secondary anaerobic fermentation bacteria, 100 parts of primary fermentation Chinese medicine extract were inoculated with 4 parts of secondary anaerobic fermentation bacteria, and anaerobically fermented at 37°C for 15 days. After the anaerobic fermentation, steam sterilization was performed with a steam temperature of 127°C and a steam pressure of 0.125 MPa for 30 minutes to obtain a secondary fermentation Chinese medicine liquid.

[0062] Step 5: Prepare the fermented liquid of Chinese herbal medicine extract

[0063] 98 parts of secondary fermented Chinese medicine liquid, 1.5 parts of sodium benzoate and 0.5 parts of propyl p-hydroxybenzoate were mixed by mass, stirred evenly, the filtrate was filtered out, and the liquid was sealed and stored to obtain the Chinese medicine plant extract fermentation liquid.

[0064] Example 3: A method for preparing a Chinese medicinal plant extract fermentation liquid, specifically comprising:

[0065] Step 1: Preparation of Chinese herbal medicine enzymatic extract

[0066] By weight, 15 parts of Bletilla striata, 30 parts of Lilium, 18 parts of Clove, 10 parts of Apricot, 25 parts of Dandelion, 25 parts of Salvia miltiorrhiza, 19 parts of Tribulus terrestris, 13 parts of Bamboo Leaf, 15 parts of White Peony Root, 15 parts of Lemon, 5 parts of Gleditsia sinensis, 15 parts of White Poria, 6 parts of Mung Bean, 3 parts of Ginkgo, 19 parts of Perilla frutescens, 11 parts of Leonurus japonicus, 6 parts of Orange Peel and 750 parts of deionized water were mixed, the pH was adjusted to 5 with food grade acetic acid, and 0.3 parts of cellulase, 0.1 parts of hemicellulase, 0.1 parts of cellulose and 0.1 parts of cellulose were added. parts of pectinase, stir evenly, and enzymolyze at 37°C for 12 hours. During the enzymolysis process, stir once every 4 hours, and stir for 10 minutes each time. After the enzymolysis is completed, boil the mixed system over high heat, and then simmer over low heat. After simmering for 90 minutes, filter out the filtrate. Add 750 parts of ionized water to the residue and boil it again over high heat. Simmer over low heat for 60 minutes and filter out the filtrate. Mix the filtrates from the two filtrations and concentrate in vacuum at 60°C until 500 parts of the filtrate remain to obtain the Chinese medicine enzymatic extract.

[0067] Step 2: Preparation of sterilized Chinese herbal medicine extract

[0068] The Chinese medicine enzymatic hydrolysis extract was sterilized by steam at a steam temperature of 127° C. and a steam pressure of 0.125 MPa for 30 minutes to obtain a sterilized Chinese medicine extract.

[0069] Step 3: Prepare primary fermentation Chinese herbal medicine extract

[0070] By mass, 1 part of brewer's yeast, 1 part of baker's yeast, and 2 parts of Candida were mixed to obtain a mixed yeast. 100 parts of sterilized traditional Chinese medicine extract were inoculated with 3 parts of the mixed yeast and aerobically fermented at 30°C for 7 days. After the aerobic fermentation, steam sterilization was carried out with a steam temperature of 127°C and a steam pressure of 0.125MPa for 30 minutes to obtain a once-fermented traditional Chinese medicine extract.

[0071] Step 4: Prepare the secondary fermentation Chinese medicine solution

[0072] By mass, 1 part of Bifidobacterium lactis, 2 parts of Streptococcus lactis, 1 part of Bifidobacterium longum, and 2 parts of Lactobacillus plantarum were mixed to obtain secondary anaerobic fermentation bacteria, and 100 parts of primary fermentation Chinese medicine extracts were inoculated with 5 parts of secondary anaerobic fermentation bacteria for treatment. The extracts were anaerobically fermented at 37°C for 15 days. After the anaerobic fermentation, steam sterilization was performed at a steam temperature of 127°C and a steam pressure of 0.125 MPa for 30 minutes to obtain secondary fermentation Chinese medicine liquid.

[0073] Step 5: Prepare the fermented liquid of Chinese herbal medicine extract

[0074] 98 parts of secondary fermented Chinese medicine liquid, 1.5 parts of sodium benzoate and 0.5 parts of propyl p-hydroxybenzoate were mixed by mass, stirred evenly, the filtrate was filtered out, and the liquid was sealed and stored to obtain the Chinese medicine plant extract fermentation liquid.

[0075] Based on Example 1, control experiments were carried out, specifically Comparative Example 1, Comparative Example 2 and Comparative Example 3, as described below:

[0076] Comparative Example 1: The Chinese medicine was not treated with enzymes, and the other conditions were the same as those in Example 1.

[0077] A method for preparing a Chinese medicinal plant extract fermentation liquid, specifically comprising:

[0078] Step 1: Prepare Chinese herbal medicine extract

[0079] By mass, 15 parts of Bletilla striata, 30 parts of Lilium, 18 parts of Clove, 10 parts of Apricot, 25 parts of Taraxacum, 25 parts of Salvia miltiorrhiza, 19 parts of Tribulus terrestris, 13 parts of Bambusa obtusifolia, 15 parts of White Peony Root, 15 parts of Lemon, 5 parts of Gleditsia sinensis, 15 parts of White Poria, 6 parts of Mung Bean, 3 parts of Ginkgo, 19 parts of Perilla frutescens, 11 parts of Leonurus japonicus, 6 parts of Tangerine Peel and 750 parts of deionized water were mixed, boiled over high heat, simmered over low heat, and the filtrate was filtered out after simmering for 90 minutes. After adding 750 parts of deionized water to the residue, the mixture was boiled again over high heat, simmered over low heat for 60 minutes, and the filtrate was filtered out. The filtrates filtered twice were mixed and concentrated in vacuo at 60°C until 500 parts of the filtrate remained to obtain a Chinese medicine extract.

[0080] Step 2: Preparation of sterilized Chinese herbal medicine extract

[0081] The Chinese medicine extract was sterilized by steam at a steam temperature of 127° C. and a steam pressure of 0.125 MPa for 30 minutes to obtain a sterilized Chinese medicine extract.

[0082] Step 3: Prepare primary fermentation Chinese herbal medicine extract

[0083] By mass, 1 part of brewer's yeast, 1 part of baker's yeast, and 2 parts of Candida were mixed to obtain a mixed yeast. 100 parts of sterilized traditional Chinese medicine extract were inoculated with 2 parts of the mixed yeast and aerobically fermented at 30°C for 7 days. After the aerobic fermentation, steam sterilization was carried out with a steam temperature of 127°C and a steam pressure of 0.125MPa for 30 minutes to obtain a once-fermented traditional Chinese medicine extract.

[0084] Step 4: Prepare the secondary fermentation Chinese medicine solution

[0085] By mass, 1 part of Bifidobacterium lactis, 2 parts of Streptococcus lactis, 1 part of Bifidobacterium longum, and 2 parts of Lactobacillus plantarum were mixed to obtain secondary anaerobic fermentation bacteria, 100 parts of primary fermentation Chinese medicine extract were inoculated with 4.5 parts of secondary anaerobic fermentation bacteria, and anaerobically fermented at 37°C for 15 days. After the anaerobic fermentation, steam sterilization was performed with a steam temperature of 127°C and a steam pressure of 0.125 MPa for 30 minutes to obtain a secondary fermentation Chinese medicine liquid.

[0086] Step 5: Prepare the fermented liquid of Chinese herbal medicine extract

[0087] 98 parts of secondary fermented Chinese medicine liquid, 1.5 parts of sodium benzoate and 0.5 parts of propyl p-hydroxybenzoate were mixed by mass, stirred evenly, the filtrate was filtered out, and the liquid was sealed and stored to obtain the Chinese medicine plant extract fermentation liquid.

[0088] Comparative Example 2: Only one aerobic fermentation was performed using mixed yeast, and the other conditions were the same as those in Example 1.

[0089] A method for preparing a Chinese medicinal plant extract fermentation liquid, specifically comprising:

[0090] Step 1: Preparation of Chinese herbal medicine enzymatic extract

[0091] By weight, 15 parts of Bletilla striata, 30 parts of Lilium, 18 parts of Clove, 10 parts of Apricot, 25 parts of Dandelion, 25 parts of Salvia miltiorrhiza, 19 parts of Tribulus terrestris, 13 parts of Bamboo Leaf, 15 parts of White Peony Root, 15 parts of Lemon, 5 parts of Gleditsia sinensis, 15 parts of White Poria, 6 parts of Mung Bean, 3 parts of Ginkgo, 19 parts of Perilla frutescens, 11 parts of Leonurus japonicus, 6 parts of Orange Peel and 750 parts of deionized water were mixed, the pH was adjusted to 5 with food grade acetic acid, and 0.3 parts of cellulase, 0.1 parts of hemicellulase, 0.1 parts of cellulose and 0.1 parts of cellulose were added. parts of pectinase, stir evenly, and enzymolyze at 37°C for 12 hours. During the enzymolysis process, stir once every 4 hours, and stir for 10 minutes each time. After the enzymolysis is completed, boil the mixed system over high heat, and then simmer over low heat. After simmering for 90 minutes, filter out the filtrate. Add 750 parts of ionized water to the residue and boil it again over high heat. Simmer over low heat for 60 minutes and filter out the filtrate. Mix the filtrates from the two filtrations and concentrate in vacuum at 60°C until 500 parts of the filtrate remain to obtain the Chinese medicine enzymatic extract.

[0092] Step 2: Preparation of sterilized Chinese herbal medicine extract

[0093] The Chinese medicine enzymatic hydrolysis extract was sterilized by steam at a steam temperature of 127° C. and a steam pressure of 0.125 MPa for 30 minutes to obtain a sterilized Chinese medicine extract.

[0094] Step 3: Prepare primary fermentation Chinese herbal medicine extract

[0095] By mass, 1 part of brewer's yeast, 1 part of baker's yeast, and 2 parts of Candida were mixed to obtain a mixed yeast. 100 parts of sterilized traditional Chinese medicine extract were inoculated with 2 parts of the mixed yeast and aerobically fermented at 30°C for 7 days. After the aerobic fermentation, steam sterilization was carried out with a steam temperature of 127°C and a steam pressure of 0.125MPa for 30 minutes to obtain a once-fermented traditional Chinese medicine extract.

[0096] Step 4: Prepare the fermented liquid of Chinese herbal medicine

[0097] 98 parts of primary fermented Chinese medicinal liquid, 1.5 parts of sodium benzoate and 0.5 parts of propyl p-hydroxybenzoate were mixed by mass, stirred evenly, the filtrate was filtered out, and the mixture was sealed and stored to obtain Chinese medicinal plant extract fermented liquid.

[0098] Comparative Example 3: Anaerobic fermentation was performed only once using anaerobic fermentation bacteria, and the other conditions were the same as those in Example 1.

[0099] A method for preparing a Chinese medicinal plant extract fermentation liquid, specifically comprising:

[0100] Step 1: Preparation of Chinese herbal medicine enzymatic extract

[0101] By weight, 15 parts of Bletilla striata, 30 parts of Lilium, 18 parts of Clove, 10 parts of Apricot, 25 parts of Dandelion, 25 parts of Salvia miltiorrhiza, 19 parts of Tribulus terrestris, 13 parts of Bamboo Leaf, 15 parts of White Peony Root, 15 parts of Lemon, 5 parts of Gleditsia sinensis, 15 parts of White Poria, 6 parts of Mung Bean, 3 parts of Ginkgo, 19 parts of Perilla frutescens, 11 parts of Leonurus japonicus, 6 parts of Orange Peel and 750 parts of deionized water were mixed, the pH was adjusted to 5 with food grade acetic acid, and 0.3 parts of cellulase, 0.1 parts of hemicellulase, 0.1 parts of cellulose and 0.1 parts of cellulose were added. parts of pectinase, stir evenly, and enzymolyze at 37°C for 12 hours. During the enzymolysis process, stir once every 4 hours, and stir for 10 minutes each time. After the enzymolysis is completed, boil the mixed system over high heat, and then simmer over low heat. After simmering for 90 minutes, filter out the filtrate. Add 750 parts of ionized water to the residue and boil it again over high heat. Simmer over low heat for 60 minutes and filter out the filtrate. Mix the filtrates from the two filtrations and concentrate in vacuum at 60°C until 500 parts of the filtrate remain to obtain the Chinese medicine enzymatic extract.

[0102] Step 2: Preparation of sterilized Chinese herbal medicine extract

[0103] The Chinese medicine enzymatic hydrolysis extract was sterilized by steam at a steam temperature of 127° C. and a steam pressure of 0.125 MPa for 30 minutes to obtain a sterilized Chinese medicine extract.

[0104] Step 3: Prepare the primary fermented Chinese medicine solution

[0105] By mass, 1 part of Bifidobacterium lactis, 2 parts of Streptococcus lactis, 1 part of Bifidobacterium longum, and 2 parts of Lactobacillus plantarum are mixed to obtain anaerobic fermentation bacteria, 100 parts of sterilized Chinese medicine extracts are inoculated with 4.5 parts of anaerobic fermentation bacteria, and anaerobically fermented at 37°C for 15 days. After the anaerobic fermentation, steam sterilization is performed at a steam temperature of 127°C and a steam pressure of 0.125 MPa for 30 minutes to obtain a once-fermented Chinese medicine liquid.

[0106] Step 4: Prepare the fermented liquid of Chinese herbal medicine

[0107] 98 parts of primary fermented Chinese medicinal liquid, 1.5 parts of sodium benzoate and 0.5 parts of propyl p-hydroxybenzoate were mixed by mass, stirred evenly, the filtrate was filtered out, and the mixture was sealed and stored to obtain Chinese medicinal plant extract fermented liquid.

[0108] Detection experiment:

[0109] Chinese medicinal plant extract fermentation broth was prepared according to the preparation methods in Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, and Comparative Example 3, respectively, and diluted with glycerol in an amount of 9 times the mass of the fermentation broth to obtain a fermentation broth sample.

[0110] Physiological toxicity test: 60 healthy adult male rats were purchased from the Experimental Animal Center of Southern Medical University, about 6 weeks old, with a single weight of 200-220 g, and were randomly divided into 6 groups, 10 rats in each group. The fermentation liquid samples were fed to the rats with food every day, and the rats were observed after 4 weeks.

[0111] Skin toxicity test: 60 healthy adult male rats were purchased from the Experimental Animal Center of Southern Medical University. They were about 6 weeks old and weighed 200-220 g. They were randomly divided into 6 groups, 10 rats in each group. The hair on both sides of the rats were shaved, and the fermentation liquid sample was applied to the skin on the same side of the rats every day, and glycerol was applied to the other side. The rats were observed after 4 weeks.

[0112] Effect test: 30 women aged 20-30, 30 women aged 30-40, and 30 women aged 40-50 from a certain region were randomly selected and divided into 6 groups, with 5 people in each age range in each group. The average ITA° value of the skin of each group was measured using an ITA° skin colorimeter, and the fermentation liquid samples were distributed and the use method was informed. After one month of use, the average ITA° value of the skin of each group was measured again, and the change rate of the average ITA° value of the skin was calculated to evaluate the whitening effect of the samples.

[0113]

[0114] Conclusion: From the test results, the fermentation liquid has no physiological toxicity and skin toxicity, and has a good cosmetic effect. The users feel good and it has a certain effect on users of different age groups.

[0115] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be considered exemplary and non-restrictive in all respects, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention.

Claims

1. A method for preparing a Chinese medicinal plant extract fermentation liquid, characterized in that: The preparation method is: Step 1: Mix the Chinese medicine composition and deionized water, adjust the pH to 4.5-5.5 with food-grade acetic acid, add the medicinal complex enzyme, stir evenly, and perform enzymolysis at 35-40° C. for 10-14 hours. During the enzymolysis process, stir once every 3-5 hours, and stir for 8-12 minutes each time. After the enzymolysis is completed, boil the mixed system over high heat, and then simmer over low heat. After simmering for 80-100 minutes, filter out the filtrate, add deionized water to the filter residue, boil it over high heat again, simmer over low heat for 50-70 minutes, and filter out the filtrate. Mix the filtrates from the two filtrations, and vacuum concentrate at 55-65° C. to obtain a Chinese medicine enzymolysis extract; Step 2: sterilize the Chinese medicine enzymatic hydrolysis extract with steam at a steam temperature of 125 to 130° C. and a steam pressure of 0.1 to 0.15 MPa for 20 to 40 minutes to obtain a sterilized Chinese medicine extract; Step 3: inoculating the sterilized Chinese medicine extract with mixed yeast, aerobically fermenting at 28-32° C. for 6-8 days, and performing steam sterilization after the aerobic fermentation, with the steam temperature being 125-130° C. and the steam pressure being 0.1-0.15 MPa for 20-40 minutes to obtain a primary fermentation Chinese medicine extract; Step 4: inoculating the primary fermented Chinese medicine extract with secondary anaerobic fermentation bacteria, anaerobically fermenting at 35-40° C. for 13-17 days, and steam sterilizing after the anaerobic fermentation, with the steam temperature at 125-130° C. and the steam pressure at 0.1-0.15 MPa for 20-40 minutes to obtain a secondary fermented Chinese medicine liquid; Step 5: Mix the secondary fermented Chinese medicine liquid, sodium benzoate and propyl parahydroxybenzoate, stir evenly, filter out the filtrate, fill and seal for storage, and obtain the Chinese medicine plant extract fermentation liquid.

2. The method for preparing a Chinese medicinal plant extract fermentation liquid according to claim 1, characterized in that: The Chinese medicine composition includes 13-17 parts of Bletilla striata, 28-32 parts of Lilium bulbs, 16-20 parts of Cloves, 8-12 parts of Apricot kernels, 23-27 parts of Taraxacum officinale, 23-27 parts of Salvia miltiorrhiza, 17-21 parts of Tribulus terrestris, 11-15 parts of Bambusa obtusifolia, 13-17 parts of White Peony Root, 13-17 parts of Lemon, 3-7 parts of Gleditsia sinensis, 13-17 parts of White Poria, 4-8 parts of Mung bean, 1-5 parts of Ginkgo, 17-21 parts of Perilla frutescens, 9-13 parts of Leonurus japonicus, and 4-8 parts of Tangerine peel.

3. The method for preparing a Chinese medicinal plant extract fermentation liquid according to claim 1, characterized in that: Calculated by weight, the medicinal complex enzyme includes 5 to 7 parts of cellulase, 1 to 3 parts of hemicellulase, and 1 to 3 parts of pectinase.

4. The method for preparing a Chinese medicinal plant extract fermentation liquid according to claim 1, characterized in that: The mass ratio of the Chinese medicine composition, deionized water and complex enzyme is (95-105):(290-310):(0.18-0.22), and the mass of deionized water added during the two decoctions is equal.

5. The method for preparing a Chinese medicinal plant extract fermentation liquid according to claim 1, characterized in that: The mixed yeast includes 0.8 to 1.2 parts of brewer's yeast, 0.8 to 1.2 parts of baker's yeast, and 1.8 to 2.2 parts of Candida.

6. The method for preparing a Chinese medicinal plant extract fermentation liquid according to claim 1, characterized in that: The mass of the inoculated mixed yeast is 1-3% of the mass of the sterilized traditional Chinese medicine extract.

7. The method for preparing a Chinese medicinal plant extract fermentation liquid according to claim 1, characterized in that: The secondary anaerobic fermentation bacteria include 0.8-1.2 parts of Bifidobacterium lactis, 1.8-2.2 parts of Streptococcus lactis, 0.8-1.2 parts of Bifidobacterium longum, and 0.8-1.2 parts of Lactobacillus plantarum.

8. The method for preparing a Chinese medicinal plant extract fermentation liquid according to claim 1, characterized in that: The mass of the inoculated secondary anaerobic fermentation bacteria is 4-5% of the mass of the primary fermentation Chinese medicine extract.

9. The method for preparing a Chinese medicinal plant extract fermentation liquid according to claim 1, characterized in that: The mass ratio of the secondary Chinese medicine fermentation liquid, sodium benzoate and propyl parahydroxybenzoate is (97-99):(1.4-1.6):(0.4-0.6).

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