Cold-resistant bacillus pumila and application thereof
CN119331751APending Publication Date: 2025-01-21NORTHEAST FORESTRY UNIV
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Patent Information
- Application Number
- CN202411156931.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-22
- Publication Date
- 2025-01-21
AI Technical Summary
Technical Problem
The prior art is difficult to effectively degrade lignin in low temperature environments, resulting in low bioenergy utilization efficiency.
Method used
A cold-tolerant Bacillus strain Pseudomonas umsongensisJ5 was screened out, which can degrade lignin in a low temperature (15°C) environment and produce highly active lignin peroxidase, manganese peroxidase and laccase.
Benefits of technology
The degradation rate of lignin reaches 18.34% at 15°C, which provides a new bacterial strain resource for low-temperature degradation of lignin and is suitable for low-temperature straw composting.
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Abstract
The invention discloses a strain of cold-resistant bacillus pumila and application thereof, and relates to the technical field of microbial degradation, and the technical scheme is as follows: the method comprises the following steps: putting a soil sample into sterilized distilled water, and carrying out shake culture under preset conditions to obtain a sample bacterial liquid; taking supernate in the sample bacterial liquid and diluting to obtain diluted bacterial liquid; adding the diluted bacterial liquid into an inorganic salt culture medium taking dealkalized lignin as a unique carbon source for coating culture to obtain bacterial colonies with different forms; and selecting bacterial colonies with different forms for streak culture, and separating to obtain the cold-resistant bacillus pumila. According to the invention, a strain of cold-resistant bacillus pumila is screened out in a low-temperature (15 DEG C) environment, a new strain resource is provided for low-temperature lignin degradation microorganisms, and the cold-resistant bacillus pumila has a good application prospect in the aspect of low-temperature straw composting.
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