Plant growth regulator for reducing deformity degree of China rose as well as application and use method of plant growth regulator

Abstract

The invention relates to the technical field of China rose cultivation, and provides a plant growth regulator for reducing the degree of deformity of China rose and application and a use method thereof, the plant growth regulator for reducing the degree of deformity of China rose comprises 5-azacytidine and polysorbate-20; c-type gene RhAG promoter region methylation caused by low temperature in the Chinese rose is inhibited through 5-azacytidine, inhibition of methylation on AG genes is relieved, the expression quantity of the AG genes is increased, the double petals degree is reduced, and the flower malformation rate is reduced; the 5-azacytidine is helped to be better dissolved in a solution; the stability and the bioavailability of the 5-azacytidine are improved. According to the plant growth regulator for reducing the deformity degree of the China rose, the problems that in the prior art, petals of the China rose in winter are excessively doubled, and the quality is seriously reduced are effectively solved, and the quality and commodity value of the China rose are effectively improved.

Description

Technical Field

[0001] The invention relates to the technical field of rose cultivation, and in particular to a plant growth regulator for reducing the degree of rose deformity, and an application and use method thereof. Background Art

[0002] Rose (Rosa hybrida), as an evergreen or semi-evergreen low shrub of the Rosaceae family, has attracted much attention because it can bloom flowers in all seasons. As an ornamental plant, its flower shape is an important factor affecting the beauty of the flower. However, the peak sales of rose cut flowers are concentrated in the period of low temperature throughout the year. During this period, due to the constraints of the low temperature environment (<10℃), the development of flower organs often becomes abnormal, such as excessive petal doubling, which in turn causes flower deformities, seriously restricting the improvement of production efficiency.

[0003] The study found that due to the high methylation of the promoter region of the C-class gene AG that regulates the development of floral organs in roses under low temperatures, the gene expression is reduced, resulting in the stamens becoming deformed petals. Therefore, how to relieve the inhibition of methylation on the AG gene is crucial to solving the problems of excessive double petals and severe quality reduction in winter roses. Summary of the invention

[0004] In order to solve the above technical problems, the purpose of the present invention is to provide a plant growth regulator for reducing the degree of rose deformity and a method of using the same. By applying the plant growth regulator, the degree of double petals can be reduced and the flower deformity rate can be reduced.

[0005] The technical solution of the present invention to solve the above technical problems is as follows:

[0006] A plant growth regulator for reducing the deformity degree of roses comprises: 5-azacytidine and polysorbate-20.

[0007] Further, from 0.1-0.8mmol·L -1 The 5-azacytidine solution was prepared by adding 0.2% polysorbate-20 to the 5-azacytidine solution.

[0008] A method for preparing a plant growth regulator for reducing the degree of rose deformity comprises the following steps:

[0009] Use water as solvent to prepare 5mmol·L -1 5-azacytidine mother solution is added to water, and then polysorbate-20 is added, and the mixture is fully shaken and mixed to obtain a plant growth regulator for reducing the degree of deformity of rose flowers.

[0010] A plant growth regulator for reducing the degree of rose deformity is used in reducing the rose deformity.

[0011] Furthermore, 5-azacytidine was used to reduce the methylation level of the promoter region of the C-class gene RhAG, thereby increasing its expression level, allowing the stamens to develop normally, reducing the degree of double petals, and reducing the rate of flower deformity.

[0012] A method for using a plant growth regulator for reducing the degree of rose deformity, wherein the plant growth regulator is sprayed on plants in the stamen primordium differentiation period to reduce the number of petalized stamens of the rose.

[0013] Furthermore, plant growth regulators are sprayed only on the tops of the flower buds.

[0014] The present invention has the following beneficial effects:

[0015] The present invention inhibits the methylation of the promoter region of the C-class gene RhAG in roses caused by low temperature by 5-azacytidine, relieves the inhibition of methylation on the AG gene, increases its expression level, normally develops stamens, reduces the degree of double petals, and reduces the flower deformity rate, thereby allowing the flower organs to develop normally, reducing the degree of rose flower deformity, and ensuring the quality of cut roses under low temperature conditions.

[0016] Among them, polysorbate-20 acts as a surfactant to help 5-azacytidine dissolve better in the solution. In addition, this ingredient can absorb moisture, keep it moist, and prevent 5-azacytidine from evaporating quickly. It can adhere to the flower buds for a long time and prolong the duration of action. Polysorbate-20 improves the stability and bioavailability of 5-azacytidine.

[0017] The rose deformity reduction plant growth regulator of the present invention has simple formula, convenient configuration, low cost and is environmentally friendly and safe.

[0018] The beneficial effects of adopting the above further scheme are: 0.1, 0.25, 0.5, 0.8mmol·L -1 5-Azacytidine solutions of 0.1mmol·L -1 μg / L 5-azacytidine solution was selected as the optimal concentration because of its best effect and lowest cost.

[0019] The plant growth regulator composition developed by the present invention has a simple preparation process, rapid effect, low cost, environmental protection and safety, and obvious effects, and can effectively reduce the petal deformity rate and improve the quality of rose cut flowers. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is a comparison diagram of the floral organ phenotypes of the present invention;

[0021] Figure 2 This is a comparative analysis diagram of the number of petalized stamens of the present invention. DETAILED DESCRIPTION

[0022] The principles and features of the present invention are described below, and the examples are only used to explain the present invention and are not used to limit the scope of the present invention. If no specific technology or conditions are specified in the embodiments, the technology or conditions described in the literature in this field or the product instructions are used. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased through regular channels.

[0023] The invention provides a plant growth regulator for reducing the deformity degree of rose. The raw materials of the plant growth regulator include 5-azacytidine and polysorbate-20, and the volume ratio of the two is 500:1.

[0024] In a specific embodiment of the present invention, the 5-azacytidine used was purchased from MedChemExpress.

[0025] Example 1: Preparation of plant growth regulator composition

[0026] (1) Add 81.9 mL of water to 100 mg of 5-azacytidine and shake thoroughly to mix well to prepare 5 mmol / L -1 A stock solution of 5-azacytidine;

[0027] (2) Add 0.2 mL of 5-azacytidine stock solution (final concentration 0.1 mmol / L) to 9.8 mL of water. -1 ), add 0.2% polysorbate-20 by solution volume, shake and mix thoroughly, and thus obtain a plant growth regulator for reducing the degree of rose deformity.

[0028] Example 2: Preparation of plant growth regulator composition II

[0029] (1) Add 81.9 mL of water to 100 mg of 5-azacytidine and shake thoroughly to mix well to prepare 5 mmol / L -1 A stock solution of 5-azacytidine;

[0030] (2) Add 0.5 mL of 5-azacytidine stock solution (final concentration 0.25 mmol / L) to 9.5 mL of water. -1 ), add 0.2% polysorbate-20 by solution volume, shake and mix thoroughly, and thus obtain a plant growth regulator for reducing the degree of rose deformity.

[0031] Example 3: Preparation of plant growth regulator composition

[0032] (1) Add 81.9 mL of water to 100 mg of 5-azacytidine and shake thoroughly to mix well to prepare 5 mmol / L -1 A stock solution of 5-azacytidine;

[0033] (2) Add 1 mL of 5-azacytidine stock solution (final concentration 0.5 mmol / L) to 9 mL of water. -1 ), add 0.2% polysorbate-20 by solution volume, shake and mix thoroughly, and thus obtain a plant growth regulator for reducing the degree of rose deformity.

[0034] Example 4: Preparation of plant growth regulator composition

[0035] (3) Add 81.9 mL of water to 100 mg of 5-azacytidine and shake thoroughly to mix well to prepare 5 mmol / L -1 A stock solution of 5-azacytidine;

[0036] (2) Add 1.6 mL of 5-azacytidine stock solution (final concentration 0.8 mmol / L) to 8.4 mL of water. -1 ), add 0.2% polysorbate-20 by solution volume, shake and mix thoroughly, and thus obtain a plant growth regulator for reducing the degree of rose deformity.

[0037] Example 5: Use of plant growth regulator composition

[0038] At 17:00 on a sunny afternoon when the plants were dry, the flower buds in the stamen primordium differentiation stage were sprayed with the plant growth regulator composition, and then placed in a cold storage for low-temperature treatment for 7 days, treated every 2 days, and continued to be placed in an incubator for culture at 25°C after the treatment. The rose plants are planted in a matrix of nutrient soil and vermiculite with a mass ratio of 3:1, and water and fertilizer management is carried out (the soil EC is kept at 1.5, the matrix pH is kept at 5.8-6.0, the watering frequency is 8-12 times, the matrix humidity is kept at 60%, and the number of fertilization and watering is adjusted according to weather conditions), and according to the growth of different pests and diseases (red spider, aphids, thrips, powdery mildew, gray mold), pesticides are applied once every 7 days (red spider medicine: avermectin, active ingredient 1.8%, spraying concentration 800 times; aphid medicine: acetamiprid, active ingredient 70%, spraying concentration 600 times; thrips medicine: bromofenac, active ingredient 5%, spraying concentration 1000 times; powdery mildew medicine: ethidium, active ingredient content 25%, spraying concentration 800 times; gray mold medicine: pyrimethanil, active ingredient content 70%, spraying concentration 1500 times) for prevention and control.

[0039] Example 6: No use of plant growth regulator composition

[0040] Choose a sunny afternoon at 17:00, when the plants are dry, and spray the flower buds in the stamen primordium differentiation stage with clean water. Do this once every 2 days. The tissue culture seedlings should be cultured in an incubator at 25°C. The rose plants are planted in a matrix of nutrient soil and vermiculite with a mass ratio of 3:1, and water and fertilizer management is carried out (the soil EC is kept at 1.5, the matrix pH is kept at 5.8-6.0, the watering frequency is 8-12 times, the matrix humidity is kept at 60%, and the number of fertilization and watering is adjusted according to weather conditions), and according to the growth of different pests and diseases (red spider, aphids, thrips, powdery mildew, gray mold), pesticides are applied once every 7 days (red spider medicine: avermectin, active ingredient 1.8%, spraying concentration 800 times; aphid medicine: acetamiprid, active ingredient 70%, spraying concentration 600 times; thrips medicine: bromofenac, active ingredient 5%, spraying concentration 1000 times; powdery mildew medicine: ethidium, active ingredient content 25%, spraying concentration 800 times; gray mold medicine: pyrimethanil, active ingredient content 70%, spraying concentration 1500 times) for prevention and control.

[0041] test:

[0042] 'Samantha' rose rooted tissue culture seedlings were used as experimental materials. 18 'Samantha' rose rooted tissue culture seedlings with basically the same size and quality were selected and divided into 6 groups, 3 in each group, and marked. The groups are as follows:

[0043] Treatment group 1: the plant growth regulator composition prepared in Example 1 was selected; the method of Example 5 was selected as the spraying method.

[0044] Treatment group 2: the plant growth regulator composition prepared in Example 2 was selected; the method of Example 5 was selected as the spraying method.

[0045] Treatment group 3: the plant growth regulator composition prepared in Example 3 was selected; the method of Example 5 was selected as the spraying method.

[0046] Treatment group 4: the plant growth regulator composition prepared in Example 4 was selected; the method of Example 5 was selected as the spraying method.

[0047] Control group 1: Select clear water as the control; select the method of Example 6 for spraying.

[0048] Control group 2: Select clear water as the control; select the method of Example 5 as the spraying method.

[0049] The first spraying time of each group was April 11, 2024, and the final treatment was completed on April 18, 2024, for a total of 3 sprayings. Take photos and record the morphological changes of the whole plant and flower organs from the time the rose buds reveal their color to the time they open. After opening, record the number of each flower organ (petals, stamens, pistils, and sepals), scan and archive. The results are as follows Figure 1 , Figure 2 , as shown in Table 1.

[0050] Table 1

[0051] Treatment Group 1 Treatment Group 2 Treatment Group 3 Treatment Group 4 Control group 1 Control group 2 Petalized stamens 9.33 13.00 16.00 16.00 13.00 27.33

[0052] like Figure 1 , Figure 2 As shown in Table 1: The plants in the treatment group were treated on April 11, 2024, and the whole plants reached the full flowering period on May 4-5, 2024; the plants in the control group were treated at the same time and reached the full flowering period on May 10-12, 2024. The number of petalized stamens in the control group 2 was significantly greater than that in the control group 1, indicating that flower deformities can be caused under such low temperature conditions. The number of petalized stamens in treatment groups 1, 2, 3 and 4 was much smaller than that in the control group 2, and was close to that in the control group 1. Among them, treatment groups 1 and 2 had significant effects. It can be seen that the plant growth regulator composition of the treatment group of the present invention can significantly reduce the number of petalized stamens, reduce the deformity rate of petals, and significantly improve the quality of roses.

[0053] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations of the present invention. A person skilled in the art may change, modify, replace and vary the above embodiments within the scope of the present invention.

Claims

1. A plant growth regulator for reducing the degree of deformity of roses, characterized in that: include: 5-Azacytidine, polysorbate 20.

2. The plant growth regulator for reducing the degree of deformity of rose according to claim 1, characterized in that From 0.1-0.8mmol·L -1 The 5-azacytidine solution is prepared by adding 0.2% polysorbate-20 to the 5-azacytidine solution.

3. The method for preparing the plant growth regulator for reducing the degree of deformity of rose according to any one of claims 1 to 2, characterized in that: The steps include: Use water as solvent to prepare 5mmol·L -1 5-azacytidine mother solution is added to water, followed by polysorbate-20, and the mixture is thoroughly shaken and mixed to obtain a plant growth regulator for reducing the degree of rose deformity.

4. Use of the plant growth regulator for reducing the degree of rose deformity as claimed in any one of claims 1 to 3 in reducing rose deformity.

5. The use according to claim 4, characterized in that By reducing the methylation level of the promoter region of the C-class gene RhAG through 5-azacytidine, its expression level was increased, the stamens developed normally, the degree of double petals was reduced, and the flower deformity rate was reduced.

6. A method for using the plant growth regulator for reducing the degree of rose deformity according to any one of claims 1 to 3, characterized in that: Spraying plant growth regulators on plants that are in the stage of stamen primordium differentiation can reduce the number of petalized stamens in roses.

7. The method of use according to claim 6, characterized in that: Spray plant growth regulators only on the tops of flower buds.

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